235 results on '"S. Andreola"'
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2. AB1018 RELATIONSHIP BETWEEN AORTIC CALCIFICATIONS AND DXA AND RADIOFREQUENCY ECHOGRAPHIC MULTI-SPECTROMETRY (REMS) ACQUISITIONS
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A. Fassio, S. Andreola, D. Gatti, M. Gatti, G. Gambaro, O. Viapiana, V. Messina, G. Zanetti, F. Pistillo, M. Rossini, and G. Adami
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundData on the relationship between peritoneal dialysis (PD), BMD and aortic calcifications (AOC) are lacking.Objectivesto study the relationship between the degree of AOC and DXA and Radiofrequency Echographic Multi-Spectrometry (REMS) acquisitions.MethodsConsecutive patients referring to the PD our clinic were enrolled. Lumbar spine (LS) and proximal femur REMS scans were performed, and LS (anteroposterior and laterolateral) and proximal femur DXA scans were performed as well. The degree of AOC was assessed through the semiquantitative score described by Kauppila et al, and applied to the laterolateral LS DXA scans. To test for correlations between different variables, we used the Pearson’s correlation for continuous variables and Spearman’s rho for discrete variables. Multiple regression analysis was performed to adjust for age and body mass index (BMI) the correlation between BMD and the CKD duration. Written informed consent was obtained from all participants (protocol 1483CESC).Results41 total patients were enrolled (29 males, 19 females). Median disease duration of CKD [IQR]: 132 months [48-140]. 15% had vertebral fractures at the DXA Vertebral Fracture Assessment (VFA). The median calcification score [IQR] was 2 [0-6] (min-max 0-20).We found a statistically significant positive correlation of moderate strength between the total calcification score and the difference between the DXA AP T-score and the DXA LL T-score at the LS (pFigure 1.scatter plot reporting the relationship between the difference between the AP and LL T-scores and the aortic calcifications score.After adjustment for confounders, we found a significant negative between the LS and femoral neck BMD measured through REMS and CKD duration (Table 1). The same correlation was not significant then BMD was assessed with DXA.Table 1.multiple linear regression analysis for REMS T-score at each site after adjustment for age and BMI. BMI, body mass index; β, standardised coefficient.REMS lumbar spine T-scoreβpCKD duration (months)-0.2280.035Age (years)-0.511BMI (kg/m2)0.566REMS femoral neck T-scoreβpCKD duration (months)-0.2100.04Age (years)-0.612BMI (kg/m2)0.484REMS total hip T-scoreβpCKD duration (months)-0.1530.219Age (years)-0.523BMI (kg/m2)0.560ConclusionOur study confirmed that the overestimation of DXA BMD assessed with the anteroposterior scan is indeed influenced by AOC. Furthermore, our data suggest that REMS might be an interesting tool for the investigation of bone changes in CKD.Disclosure of InterestsAngelo Fassio: None declared, Stefano Andreola: None declared, Davide Gatti Speakers bureau: Amgen, Celgene Eli-Lilly, MSD-Italia, Organon, UCB, Paid instructor for: Amgen, Celgene Eli-Lilly, MSD-Italia, Organon, UCB, Matteo Gatti: None declared, Giovanni Gambaro Speakers bureau: Vitor Pharma, Ombretta Viapiana: None declared, Valeri Messina: None declared, Giulia Zanetti: None declared, Francesca Pistillo: None declared, Maurizio Rossini Speakers bureau: Abiogen, Amgen, Abbvie, BMS, Celgene, Eli-Lilly, Galapagos, Grunenthal, MSD, Novartis, Pfizer, Sanofi, Sandoz, Theramex, UCB., Giovanni Adami: None declared more...
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- 2022
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Catalog
3. AB1017 RADIOFREQUENCY ECHOGRAPHIC MULTI-SPECTROMETRY (REMS) AND DUAL-ENERGY X-RAYS ABSORPTIOMETRY FOR THE EVALUATION OF BONE MINERAL DENSITY IN A PERITONEAL DIALYSIS SETTING
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A. Fassio, S. Andreola, D. Gatti, M. Gatti, G. Gambaro, M. Rossini, O. Viapiana, G. Zanetti, F. Pistillo, V. Messina, and G. Adami
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Rheumatology ,Immunology ,Immunology and Allergy ,General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundRadiofrequency echographic multi-spectrometry (REMS) is a novel ultrasound-based technique that has shown good reliability in the assessment of bone mineral density (BMD).ObjectivesThe aim of this study was to compare the performance of the REMS BMD assessment with dual-energy X-rays absorptiometry (DXA) in a cohort of patients affected by end-stage renal disease undergoing peritoneal dialysis (PD).MethodsConsecutive patients referring to the PD clinic of our hospital were enrolled. Lumbar spine and proximal femur REMS scans were performed, and lumbar spine (anteroposterior and laterolateral) and proximal femur DXA scans were performed as well. Clinical data were extracted from medical records. The risk assessment outputs of two fracture risk algorithms (FRAX and DeFRA), calculated upon the worst BMD obtained from either technique were compared as well. Analysis of variance (ANOVA) with post hoc analysis (Bonferroni) and a two-sided Student’s t-test were used to estimate the absolute differences between groups.Written informed consent was obtained from all participants included (protocol 1483 CESC).Results41 total patients were enrolled (Table 1). No significant differences were documented between the BMD T-scores measured through DXA or REMS at the proximal femur. At the lumbar spine, the DXA laterolateral T-score was not significantly different from that of REMS, while the DXA anteroposterior T-score was significantly higher than both the anteroposterior DXA and the REMS measurements (Figure 1, panel A and B). When either DXA or REMS was adopted, no significant difference in the fracture risk estimate was found for both algorithms (Figure 1, panel C and D).Table 1.anthropometrics, clinical and biochemical characteristics of the enrolled sample. CKD, chronic kidney disease; PTH, parathyroid hormone; ALP, alkaline phosphatase; IQR, interquartile range; VFA, vertebral fracture assessment.Sample size (M)41 (29)Age (y)Median [IQR]62 [52-73]Height (cm)Median [IQR]170 [165-176]Body weight (Kg)Median [IQR]74 [61-83]Body mass index (Kg/m2)Median [IQR]25 [22-27.8]Disease duration – CKD (months)Median [IQR]132 [48-140]Dialysis duration (months)Median [IQR]10 [3-24]S-calcium (mg/dL)Median [IQR]9.1 [8.6-9.4]S-phosphorous (mg/dL)Median [IQR]5.4 [4.6-6.4]PTH (pg/mL)Median [IQR]31.4 [22.8-46.8]25OH Vitamin D (nmol/L)Median [IQR]53 [36-72]Patients with morphometric fractures (VFA)15%Patients with femoral fractures2.4%Total n° of morphometric fractures12Figure 1.comparison of the mean T-scores (error bars represent 95%CI) measured with DXA and REMS at the lumbar spine (panel A), and at the femur (panel B). Comparison between the DeFRA DXA and REMS-derived outputs (panel C) and FRAX DXA and REMS-derived outputs (panel D) raw and after correction for TBS. DeFRA, FRAX-derived risk assessment tool; FRAX, Fracture Risk Assessment tool; AP, anteroposterior; LL, latero-lateral; TH, total hip; FN, femoral neck; TBS, trabecular bone score.ConclusionOur data showed a good agreement, in a real-life PD setting, between the DXA and REMS-derived BMDs and in the consequent fracture risk assessment obtained with the FRAX or DeFRA tools.Disclosure of InterestsAngelo Fassio: None declared, Stefano Andreola: None declared, Davide Gatti Paid instructor for: Amgen, Celgene Eli-Lilly, MSD-Italia, Organon, UCB., Consultant of: Amgen, Celgene Eli-Lilly, MSD-Italia, Organon, UCB., Matteo Gatti: None declared, Giovanni Gambaro Speakers bureau: Vifor Pharma, Maurizio Rossini Speakers bureau: Abiogen, Amgen, Abbvie, BMS, Celgene, Eli-Lilly, Galapagos, Grunenthal, MSD, Novartis, Pfizer, Sanofi, Sandoz, Theramex, UCB., Ombretta Viapiana: None declared, Giulia Zanetti: None declared, Francesca Pistillo: None declared, Valeri Messina: None declared, Giovanni Adami: None declared more...
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- 2022
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4. Reoperation for locally recurrent breast cancer in patients previously treated with conservative surgery
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B Salvadori, F. Cusumano, S. Andreola, Rosalba Miceli, R. Zucali, Umberto Veronesi, A. R. Conti, and Ettore Marubini
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Reoperation ,medicine.medical_specialty ,medicine.medical_treatment ,Breast Neoplasms ,Disease-Free Survival ,Breast cancer ,Breast-conserving surgery ,Humans ,Medicine ,Total Mastectomy ,Mastectomy ,business.industry ,Axillary Lymph Node Dissection ,medicine.disease ,Combined Modality Therapy ,Survival Analysis ,Surgery ,Radiation therapy ,Female ,Neoplasm Recurrence, Local ,business ,Complication ,Quadrantectomy ,Follow-Up Studies - Abstract
Background This study aimed to analyse the possibility of surgical rescue of intrabreast tumour recurrence (IBTR) following conservative operation for breast cancer, i.e. quadrantectomy, axillary dissection and radiotherapy. Methods Of 2544 patients treated with this approach, 209 presented with an IBTR as the first and only sign of relapse. Some 197 patients were considered suitable for further surgery; 12 were inoperable. Six patients declined operation. Results Reoperative surgery was total mastectomy in 134 patients (70 per cent) and further local resection in 57 (30 per cent). Median follow-up after second surgery was 73 (range 1–192) months. The overall survival probability at 60 months was 70 per cent after mastectomy and 85 per cent following further local excision. There was no difference in disease-free survival between the two operative groups. Second IBTR was more common at 5 years in the re-excision group (19 versus 4 per cent). Conclusion Since the type of surgery did not seem to affect survival, breast conservation can be considered in selected patients with IBTR. more...
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- 1999
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5. Percutaneous treatment of small hepatic tumors by an expandable RF needle electrode
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Elisabetta Buscarini, M. Di Stasi, M. Rago, A. Zangrandi, Francesco Garbagnati, S. Andreola, Pietro Quaretti, D E Silverman, Sandro Rossi, and Luigi Buscarini
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Male ,Surgical resection ,Needle electrode ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Percutaneous ,Thermal ablation ,Punctures ,Electrocoagulation ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Aged ,Ultrasonography ,Aged, 80 and over ,Mean diameter ,business.industry ,Liver Neoplasms ,Nodule (medicine) ,General Medicine ,Middle Aged ,medicine.disease ,Needles ,Hepatocellular carcinoma ,Female ,Dynamic ct ,Radiology ,medicine.symptom ,Tomography, X-Ray Computed ,business - Abstract
The aim of this study was to evaluate the usefulness of expandable RF needle electrodes in the treatment of hepatic cancer.Thirty-seven patients, 23 of whom had 26 hepatocellular carcinoma nodules and 14 of whom had 19 hepatic metastatic nodules, underwent treatment by RF interstitial thermal ablation with expandable needle electrodes. Forty-five tumor nodules were treated in 64 RF interstitial thermal ablation sessions with 83 needle electrode insertions. The mean diameter of the tumor nodules was 2.5 cm (range, 1.1-3.5 cm). Immediate posttreatment tumor necrosis was evaluated by dynamic CT in all cases. Two patients with hepatocellular carcinoma and three patients with metastases underwent surgical resection 20-60 days after RF treatment. The remaining 32 patients were followed up clinically.The mean number of RF interstitial thermal ablation sessions to complete tumor nodule treatment was 1.4. Mean number of needle electrode insertions was 1.8. No complications were observed. Posttreatment dynamic CT showed a completely nonenhancing area in the site of the treated tumor in 44 of 45 cases. The remaining patient with metastatic disease had persistent enhancing tissue. Histology showed complete necrosis in four treated tumor nodules and residual viable cancer in one. Twenty-one patients with hepatocellular carcinoma were followed up for 6-19 months (mean, 10 months). Of these patients, six showed recurrences and 15 remained apparently disease-free. Two patients died, one from advanced cancer and one from other causes. Eleven patients with hepatic metastases were followed up for 7-20 months (mean, 12 months). Of these patients, nine showed recurrent disease and only two remained apparently disease-free. Two patients died from disseminated disease.RF interstitial thermal ablation of hepatic tumor by expandable needle electrodes is a safe and effective technique. Local ablation of tumors not exceeding 3.5 cm in diameter is achieved in a short time without complications. more...
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- 1998
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6. Sudden death from systemic sarcoidosis: a case of legal medicine
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R, Zoja, S, Andreola, G, Gentile, and A, Rancati
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Central Nervous System ,Death, Sudden ,Young Adult ,Fatal Outcome ,Sarcoidosis ,Central Nervous System Diseases ,Cause of Death ,Humans ,Female ,Autopsy ,Forensic Pathology - Abstract
The sarcoid condition of vital organs such as heart, lungs, liver and brain, may constitute, though rarely, a dangerous situation for survival. In forensic pathology, sudden death related to such disease represents an unusual event occurring in subjects who die unexpectedly in spite of their previous good health, and whose autopsy reveals, mainly, the involvement of heart or the central nervous system (CNS).The Authors describe a case of sudden death due to systemic sarcoidosis with atypical presentation in a young South American nulliparous woman showing, as the only symptom, occasional episodes of spotting during the last two months of her life.The only noteworthy finding from the autopsy resulted in secondary obstructive hydrocephalus. The subsequent forensic toxicological examination was negative, whereas the histopathological examination, conducted by means of post-fixation techniques and standard coloring methods on the viscera removed during autopsy, revealed useful data to determine the cause of death, consisting of a diffuse inflammatory reaction with giant cells and epithelioid cells of sarcoid type localized in the CNS and in the genitourinary apparatus.The case presented, ranking among deaths occurred unexpectedly, is a useful opportunity both for clinical remarks, given its inherent diagnostic difficulties, especially in the presence of atypical symptoms, and for legal medicine ones, given the inclusion of sarcoidosis in cases of sudden death that can give rise to criminal investigations. more...
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- 2013
7. A series of 11 patients with angiosarcoma of the breast
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R. Saccozzi, F. Cusumano, B. Salvadori, S. Andreola, and R. Del Bo
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medicine.medical_specialty ,Series (stratigraphy) ,medicine.diagnostic_test ,business.industry ,Adjuvant chemotherapy ,Variable size ,Postoperative radiotherapy ,Disease free ,General Medicine ,Surgery ,Tumor grade ,Biopsy ,Medicine ,Angiosarcoma ,business - Abstract
11 patients with angiosarcoma of the breast have been reviewed. All patients were females, age range 18–45 years and all presented with a palpable breast mass of variable size ranging from 2–20 cm in diameter. Distant metastases were present at the time of diagnosis in one patient. All patients underwent surgery including seven total mastectomies, three wide local excisions and one biopsy. Three patients received postoperative radiotherapy and three received adjuvant chemotherapy. The median duration of follow-up was 60 months. Five patients were alive and disease free at 18, 44, 47 and 124 months. There was no evident correlation between tumour grade and survival but there was a relationship between tumour size and outcome, the four patients who presented with the largest tumours (> 6 cm) all dying from their disease. more...
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- 1993
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8. Primary anorectal melanomas: an istitutional experience
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C, Rossetti, D, Koukouras, M, Eboli, S, Andreola, and L, Bertario
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Adult ,Aged, 80 and over ,Male ,Humans ,Female ,Middle Aged ,Anus Neoplasms ,Melanoma ,Aged ,Neoplasm Staging - Abstract
Primary melanomas (M) of the rectum and anal canal are a rare pathological event, constituting approximately 1% of all invasive tumors in this site. From January 1973 to December 1990 at the Istituto Nazionale per lo Studio e 1a Cura dei Tumori of Milan, 11 patients were treated for M (5 males and 6 females), with a mean age of 60 years (range 40-80). The site of origin of the M was rectal in four patients, anal in five patients and in the anorectal joint in two patients. The lesion was prevalently polypoid and the average size was 4 cm (1-7.5 cm). Symptoms referred by the patients were rectal bleeding and tenesmus. In one patient the diagnosis was made after biopsy of an inguinal metastatic lymphnode. Of the 11 patients, six underwent curative resection (four Miles' resections and two local excisions). One patient is still alive with no evidence of disease after 120 months. The remaining five patients were submitted to palliative treatment, due to the presence of metastases in four of them and to age and general conditions in one. All of these patients died at 1, 2, 4, 5, and 6 months (median: 4 months). Overall median survival was eight months: 20 months in the radically treated group and four months in the palliatively treated group. Our data are in agreement with those reported in literature and confirm the prognostic severity of anorectal M due both to late diagnosis and the biological aggressiveness of the neoplasm. more...
- Published
- 1997
9. Acute confusional state with fatal outcome in a cancer patient
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Luigi Celio, Augusto Caraceni, S. Andreola, and Fabio Simonetti
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Pediatrics ,medicine.medical_specialty ,Fatal outcome ,Neurology ,business.industry ,Cancer ,Dermatology ,General Medicine ,medicine.disease ,Surgery ,Psychiatry and Mental health ,medicine ,Neurology (clinical) ,Mesothelioma ,Neurosurgery ,medicine.symptom ,business ,Confusion ,Neuroradiology - Published
- 2004
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10. Treatment of malignant polyps
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P, Spinelli, P, Pizzetti, P, Sala, S, Andreola, and L, Bertario
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Adult ,Aged, 80 and over ,Male ,Cell Transformation, Neoplastic ,Treatment Outcome ,Adenomatous Polyposis Coli ,Predictive Value of Tests ,Humans ,Female ,Colonoscopy ,Middle Aged ,Aged ,Retrospective Studies - Abstract
From 1975 to 1993, at the Division of Diagnostics and Endoscopic Surgery of the Istituto Nazionale Tumori in Milan, 191 malignant adenomas of colon-rectum have been endoscopically removed. On the basis of histopathologic criteria, endoscopic treatment has been judged adequate in 102 cases (53%), not adequate in 44 (23%), doubtfully adequate in 45 (24%). In 84 patients (44%) endoscopic polypectomy has been followed by surgical resection of the involved intestinal tract, 107 patients (56%) have been treated only by endoscopy. Results have been evaluated on the basis of surgical specimen, clinical follow-up and survival, showing that criteria we have adopted for the adequacy of the endoscopic treatment have a high negative predictive value (96%) and a low positive predictive value (32%). Actuarial survival of patients treated only endoscopically is 97% at 5 years and 95% at 10 years. more...
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- 1995
11. PRELIMINARY-RESULTS OF PREOPERATIVE AXILLARY RADIOIMMUNOSCINTIGRAPHY WITH IN-111 B72.3 IN BREAST-CANCER
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Arturo Chiti, Roberto Agresti, Flavio Crippa, Marco Greco, Umberto Veronesi, Emilio Bombardieri, Ettore Seregni, S. Andreola, and G.L. Buraggi
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Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,Cancer ,medicine.disease ,Molecular medicine ,Tumor site ,Axillary Metastasis ,Axilla ,medicine.anatomical_structure ,Breast cancer ,Internal medicine ,medicine ,Axillary nodes ,In patient ,Nuclear medicine ,business - Abstract
Nineteen patients with clinical and mammographic diagnosis of breast cancer and 1 patient already mastectomized underwent radioimmunoscintigraphy (RIS) in order to evaluate the axillary node status before surgery. After histologic examination, ductal breast carcinomas were found in 17/19 patients and axillary metastases were found in 11 patients. RIS was performed with planar scintigraphies and SPET (Single Photon Emission Tomography) of mammary and axillary regions after intravenous injection of In-111 B72.3. Overall RIS sensitivity for breast tumors was 71% (12/17); according to the tumor site different RIS sensitivity was demonstrated (90% for lesions of external quadrants versus 43% for lesions of internal quadrants). RIS was negative in 2 patients with post-surgery diagnosis of mammary dysplasia. As regards axillary metastasis RIS sensitivity and specificity were 91% (10/11) and 78% (7/9), respectively. Human Anti-Murine Antibody (HAMA) production was shown in 16.7% (2/12) of the patients. The quantitation of In-111 B72.3 uptake (%I.D./g; mean+/-S.D.) was 0.0054+/-0.0021 in breast tumors, 0.0021+/-0.0011 in normal mammary tissue, 0.0053+/-0.0027 in axillary metastasis and 0.0032+/-0.008 in normal axillary nodes. Our data demonstrated that RIS can detect tumor spread to the axilla in patients with breast cancer. Larger pre-operative study is required to evaluate if RIS can alter the management of this disease. more...
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- 1995
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12. Antibody response against the c-erbB-2 oncoprotein in breast carcinoma patients
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S M, Pupa, S, Ménard, S, Andreola, and M I, Colnaghi
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Receptor, ErbB-2 ,Carcinoma, Ductal, Breast ,Plasma Cells ,Breast Neoplasms ,Cell Transformation, Viral ,Antibodies ,ErbB Receptors ,Molecular Weight ,Proto-Oncogene Proteins ,Antibody Formation ,Tumor Cells, Cultured ,Humans ,Female ,Lymphocytes - Abstract
Indirect immunofluorescence analysis of sera from breast carcinoma patients whose tumors were characterized for overexpression of the c-erbB-2 oncoprotein (p185HER2) and for lympho-plasma cell infiltration, revealed no circulating antibodies specifically directed against the p185HER2 molecule in the 20 samples tested, whereas supernatants of B-cell clones, derived from Epstein-Barr virus-transformed peripheral blood lymphocytes from 10 of these patients, contained such antibodies in 6 of the 7 c-erbB-2- and lympho-plasma cell infiltration-positive cases. The antibodies contained in two of the positive supernatants immunoprecipitated a M(r) 185,000 molecule from oncoprotein-positive cell extracts that was identified as the oncoprotein in sequential immunoprecipitation experiments with anti-p185HER2 monoclonal antibodies. No cells producing antibodies with a similar reactivity were obtained from Epstein-Barr virus-transformed peripheral blood lymphocytes from breast carcinoma patients with p185HER2-negative tumors or from healthy donors. These data prove the existence of an antibody response specifically directed against the p185HER2 oncoprotein in breast carcinoma patients that may represent an important effector mechanism in the control of c-erbB-2-overexpressing tumors. more...
- Published
- 1993
13. Total rectal resection, colo-endoanal anastomosis and colic reservoir for cancer of the lower third of the rectum
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E, Leo, F, Belli, M T, Baldini, M, Vitellaro, N, Santoro, L, Mascheroni, S, Andreola, M, Bellomi, G, Rebuffoni, and R, Zucali
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Adult ,Male ,Reoperation ,Colon ,Rectal Neoplasms ,Anastomosis, Surgical ,Anal Canal ,Middle Aged ,Sexual Dysfunction, Physiological ,Postoperative Complications ,Treatment Outcome ,Colostomy ,Humans ,Female ,Aged - Abstract
The treatment of low rectal cancer is still a widely debated topic in surgical oncology. From March 1990 to August 1991, 18 patients with tumors sited in the lower third of the rectum underwent a total rectal resection extended to the ano-rectal junction. As restorative procedure, a colic J-shaped pouch and a handsewn pouch-endoanal anastomosis was adopted. All the lesions were less than 8 cm from the anal verge; in 94.5% the distal tumor margin was located within 6.5 cm of the cutaneous edge. Histological clearance of the rectum cut edge was documented in all cases. Only one patient (Dukes C) relapsed four months later at the para-anastomotic level. No mortality or major complications related to surgical procedure were found. In 13 patients perfect continence was achieved and in 12 cases less than two bowel movements a day were recorded. No one complained of severe sexual dysfunction. All patients are still alive. The follow up ranged from 6 to 22 months (median: 12). This experience together with data obtained from last years' literature indicate that a conservative surgical procedure, as total rectal resection and colo-anal anastomosis, can be considered a feasible and radical option for treatment of low rectal cancer. more...
- Published
- 1993
14. Confirmation of graft-versus-host disease after liver transplantation by PCR HLA-typing
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V, Mazzaferro, S, Andreola, E, Regalia, F, Poli, R, Doci, F, Bozzetti, and L, Gennari
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Male ,Base Sequence ,HLA Antigens ,Molecular Sequence Data ,Graft vs Host Disease ,Humans ,HLA-DR Antigens ,Middle Aged ,Polymerase Chain Reaction ,Liver Transplantation - Published
- 1993
15. How long is a 6 cm margin of resection in the stomach?
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F, Bozzetti, G, Bonfanti, E, Regalia, S, Andreola, R, Doci, G, La Malfa, and L, Gennari
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Adult ,Male ,Gastrectomy ,Stomach Neoplasms ,Stomach ,Humans ,Female ,Prospective Studies ,Middle Aged ,Aged - Abstract
In order to assess whether the gastric wall undergoes some change in length during gastrectomy operation for cancer, we measured the variation in length of the anterior gastric wall in 25 patients. The first measurement was made at the beginning of laparotomy by placing two stitches on the anterior gastric wall and registering the distance between them. A second and a third measurement were recorded when the stomach was fully isolated just before its transection and subsequently on the anatomic table. The results indicate that the usual recommendations made by pathologists to maintain a 6 cm margin of tissue clearance proximally to the cephalic edge of the tumour, can be safely followed by the surgeons who can correctly assess, during operation, the distance between tumour and the desirable line of transection since no misleading reduction in size of the resected specimen takes place. more...
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- 1992
16. Evaluation of resection margins after breast conservative surgery with monoclonal antibodies
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U, Veronesi, G, Farante, V, Galimberti, M, Greco, A, Luini, V, Sacchini, S, Andreola, F, Leoni, S, Ménard, and M, Ronco
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Predictive Value of Tests ,Antibodies, Monoclonal ,Fluorescent Antibody Technique ,Humans ,Breast Neoplasms ,Female ,Mastectomy, Segmental - Abstract
An immunocytochemical method for the detection of cancer cells, in the cell suspension obtained by scraping the surface of the surgical resection margins is described and its sensitivity compared to the conventional histology performed on random biopsies from the same margins. The reactivity of the cells with a pool of monoclonal antibodies (Mab) directed against epithelial markers indicated that in 80% of the 42 cases tested, the scraping method was adequate for the gathering of cells from the margins. The analysis of the samples using B72.3 Mab specific for tumor cells revealed that, among B72.3-positive tumor cases, 31% of breast margins contained tumor cells, whereas only 12% were histologically positive. Our results indicate that the immunocytological methodology is therefore more sensitive and should be used alongside histological examination to detect the tumor contamination in the surgical resection margins. more...
- Published
- 1991
17. Experience with radiofrequency ablation of hepatocellular carcinoma in patients awaiting liver transplantation
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Andrea Pulvirenti, Vincenzo Mazzaferro, D. Sarli, S. Andreola, Francesco Garbagnati, S. Perrone, Jorgelina Coppa, Enrico Regalia, M. Schiavo, R. Romito, T. Camerini, and Alfonso Marchianò
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medicine.medical_specialty ,Hepatology ,business.industry ,Radiofrequency ablation ,medicine.medical_treatment ,Liver transplantation ,medicine.disease ,law.invention ,law ,Hepatocellular carcinoma ,medicine ,In patient ,Radiology ,business - Published
- 2003
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18. 8Experience with liver transplantation for hepatocellular carcinoma in cirrhosis
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Vincenzo Mazzaferro, S. Andreola, M Deipoli, Jorgelina Coppa, Mario Ammatuna, Enrico Regalia, Andrea Pulvirenti, M. Schiavo, and R. Romito
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Transplantation ,medicine.medical_specialty ,Cirrhosis ,Hepatology ,business.industry ,medicine.medical_treatment ,Liver transplantation ,medicine.disease ,Gastroenterology ,Hepatocellular carcinoma ,Internal medicine ,medicine ,Surgery ,business - Published
- 2000
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19. 697 Local recurrence after total rectal resection, mesorectum excision and coloendoanal anastomosis for treatment of low rectal cancer
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E. Leo, F. Belli, S. Andreola, M.T. Baldini, G. Gallina, T. Redaelli, and R. Giovanazzi
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Cancer Research ,Oncology - Published
- 1995
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20. The outpatient-procedure effectiveness of laser treatments in oncology
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S. Andreola, H. Emanuelli, L. Luciani, G. Bandieramonte, Fausto Chiesa, R. Marchesini, S. Di Pietro, and Alberto Azzarelli
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Laser surgery ,medicine.medical_specialty ,Co2 laser ,Outpatient procedure ,Genital Neoplasms, Female ,business.industry ,medicine.medical_treatment ,Postoperative pain ,Soft tissue ,Breast Neoplasms ,Dermatology ,Ambulatory Surgical Procedure ,Oral cavity ,Surgery ,Laser treatments ,Ambulatory Surgical Procedures ,Head and Neck Neoplasms ,Neoplasms ,medicine ,Humans ,Female ,Laser Therapy ,business - Abstract
The outpatient procedure of CO2 laser surgery has been recently integrated into the routine activity of the National Cancer Institute, Milan. Specific operative protocols that are codified for laser outpatient selection after adequate staging by pathology are presented. Specific indications for gynecologic disease (preneoplastic disorders, CIN, VAIN, and VIN), and general-surgery neoplastic disorders (soft tissue sarcomas, lip and oral cavity lesions, breast lesions) are described. Details of the safety procedures, rules of application, laser instrumentation accessories and wattages, both with vaporization and excisional technique, are reported. An overall rate of 30% out of the 4,000 cases treated yearly are selected for laser treatment procedures on the basis of the evaluation of real advantages. The general advantages of laser surgical applications such as reduction of bleeding and postoperative pain, early and late complications; no-touch method; and patient acceptability--have been emphasized in the routine outpatient procedures. Reduction of the total costs and of the waiting list for hospitalization by the new procedures are finally considered. more...
- Published
- 1983
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21. Arbuscular mycorrhizal fungi on the development and copper content in corn and sorghum plants
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S. Barros, R. Turchetto, J. B. Magalhães, E. Canepelle, D. S. Andreola, C. O. Ros, C. J. Basso, V. R. Silva, and R. F. Silva
- Subjects
Sorghum bicolor L. ,phytostabilization ,heavy metal ,mycorrhiza ,polluted soil ,Zea mays L ,Science ,Biology (General) ,QH301-705.5 ,Zoology ,QL1-991 ,Botany ,QK1-989 - Abstract
Abstract The concentration of copper in the soil increased with the intensification of agricultural activities, mainly in grape production areas and orchards as a result of the application of pesticides. Arbuscular mycorrhizal fungi make up the microbial biomass of the soil and appear as an alternative to be researched for the development of plants in an environment contaminated with copper. The purpose of this pot study was to analyze the influence of arbuscular mycorrhizal fungi on the development and content of copper in corn and sorghum plants. Soil treatments were: without inoculum (control) and two arbuscular mycorrhizal (Acaulospora scrobiculata and Rhizoglomus clarum) and five doses of copper (0, 100, 200, 300, and 400 mg Cu kg-1 soil); with seven repetitions. Plant height, stem diameter, number of tillers, root volume, shoot and root dry weight yields, shoot, root and grain Cu concentrations, pseudo-total soil Cu, percentage of mycorrhizal colonization and relative mycorrhizal efficiency index in reducing Cu concentration in root and shoot of corn and sorgum were evaluated. Morphological parameters of sorghum and corn were reduced with at high Cu doses in the soil, and the inoculation with Acaulospora scrobiculata and Rhizoglomus clarum resulted in greater development and lower Cu concentration in the dry mass of the shoot and root parts sorghum and corn plants. more...
- Published
- 2024
- Full Text
- View/download PDF
22. Prognosis of patients after palliative surgical procedures for carcinoma of the stomach
- Author
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F, Bozzetti, G, Bonfanti, R A, Audisio, R, Doci, G, Dossena, L, Gennari, and S, Andreola
- Subjects
Stomach Neoplasms ,Carcinoma ,Liver Neoplasms ,Palliative Care ,Methods ,Humans ,Neoplasm Recurrence, Local ,Prognosis - Abstract
From 1965 to 1980, 294 (33 per cent) of 885 patients admitted to the Istituto Nazionale Tumori of Milan with carcinoma of the stomach did not undergo a radical operation. Forty-eight patients were not considered suitable for operation due to their poor general status and spread of disease. One hundred and five patients underwent simple exploratory laparotomy, 80 underwent bypass procedure and 61, noncurative resection, and the operative mortality rate was 4.7, 10.0 and 11.5 per cent, respectively. Median survival time was 2.4 months for patients who did not undergo an operation, 2.8 months after exploratory laparotomy and 3.5 months after bypass procedures. Median survival time after nonradical resections was 8.0 months and 6 per cent of these patients survived for more than five years. To perform the analysis within relatively homogeneous groups, patients with different treatments were further stratified into three groups according to the spread of disease: local, distant and local plus distant spread. It is noteworthy that the benefit after bypass procedures in comparison to exploratory laparotomy was limited to those patients with local spread of disease; the advantage of nonradical resection was apparent in all groups. Survival time was finally analyzed with reference to the extent of liver involvement in patients with metastatic disease confined to the liver. The data suggest that a limited sporadic survival time is possible only after gastric resection in patients with liver involvement of less than 50 per cent. more...
- Published
- 1987
23. DNA ploidy of human breast cancer
- Author
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G, Del Bino, R, Silvestrini, M R, Zucconi, G, Marchese, B, Valentinis, G, Di Fronzo, and S, Andreola
- Subjects
Adult ,Aged, 80 and over ,Ploidies ,Breast Neoplasms ,DNA, Neoplasm ,Middle Aged ,Cell Fractionation ,Receptors, Estrogen ,Lymphatic Metastasis ,Freezing ,Humans ,Female ,Receptors, Progesterone ,Aged - Abstract
Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement or tumor size, although the highest frequencies were observed in large tumors (88%) or with more than 10 positive nodes (77%). Aneuploidy was more frequently observed in ductal infiltrating (81%) than in lobular histology and in tumors lacking both progesterone and estrogen receptors (85%). Analysis of ploidy in primary and synchronous lymph node metastases from the same patient showed a high agreement rate (90%) of DNA patterns simply defined as diploid or aneuploid. However, differences in DNA stemlines and DNA indices between the two synchronous lesions from the same patient were a rather frequent event. more...
- Published
- 1989
24. Gastric carcinoid with unusual ultrastructural features
- Author
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L, Lombardi and S, Andreola
- Subjects
Adult ,Microscopy, Electron ,Histocytochemistry ,Stomach Neoplasms ,Humans ,Female ,Carcinoid Tumor ,Immunohistochemistry - Published
- 1988
25. The importance of pathology in prognosis and management of breast cancer
- Author
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F, Rilke, S, Andreola, A, Carbone, C, Clemente, and S, Pilotti
- Subjects
Adult ,Hyperplasia ,Biopsy, Needle ,Breast Neoplasms ,Middle Aged ,Prognosis ,Epithelium ,Neoplasms, Multiple Primary ,Carcinoma, Intraductal, Noninfiltrating ,Receptors, Estrogen ,Sex Chromatin ,Lymphatic Metastasis ,Humans ,Female ,Carcinoma in Situ ,Aged - Published
- 1978
26. Hormone steroid receptor variation after tamoxifen administration in endometrial adenocarcinoma from postmenopausal patients
- Author
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G, Di Fronzo, E, Ronchi, V, Cappelletti, L, Luciani, D, Coradini, S, Oriana, P, Miodini, and S, Andreola
- Subjects
Tamoxifen ,Neoplasms, Hormone-Dependent ,Receptors, Estrogen ,Uterine Neoplasms ,Humans ,Female ,Adenocarcinoma ,Menopause ,Middle Aged ,Receptors, Progesterone - Abstract
Cytoplasmic and nuclear variations of estrogen (ER) and progesterone receptors (PgR) induced by tamoxifen (TAM) treatment were investigated in 38 postmenopausal women with endometrial carcinoma. The treatment consisted of a daily oral administration of 40 mg for 7 days. Tumor samples from each patient were withdrawn before TAM administration under hysteroscopy and at the time of hysterectomy. Cytoplasmic and nuclear receptors were respectively determined by the dextran-coated charcoal assay and the hydroxylapatite technique. In the cytoplasmic fraction no significant change in mean ER value was observed, but a statistically significant increase in PgR was found (P less than 0.001). Conversely, in the nuclear fraction PgR did not vary, but a significant increase in ER content (P less than 0.001) was observed. PgR, analyzed by sucrose density gradient after TAM, showed the same sedimentation property (9 S) as the preexisting receptor, but with a single peak profile, indicating a lower heterogeneity. Our data support the hypothesis that the mechanism of PgR synthesis is induced by the ER-TAM complex and suggest the possibility of increasing the PgR content in these patients for sequential endocrine therapy. more...
- Published
- 1986
27. Selective atrophy of the type IIb muscle fibers in rheumatoid arthritis and progressive systemic sclerosis (scleroderma). A biopsy histochemical study
- Author
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M G, Fiori, S, Andreola, G, Ladelli, and M R, Scirea
- Subjects
Adult ,Arthritis, Rheumatoid ,Male ,Muscular Atrophy ,Scleroderma, Systemic ,Biopsy ,Muscles ,Humans ,Female ,Middle Aged ,Aged - Abstract
We have examined biopsy material from the left m. vastus lateralis of eight patients suffering from rheumatoid arthritis and four patients with progressive systemic sclerosis (scleroderma). All were chosen according to the duration and the severity of disease, so that the broadest possible spectrum of signs and symptoms could be considered. Muscular specimens showed a selective and constant atrophy of the type IIB fibers, as revealed by the myofibrillar ATPase histochemical reaction (both at a pH of 9.4 and with pre-incubation at pH 4.35 and 4.63). Atrophy of the type I fibers was seen only occasionally. Neither structural abnormalities, such as 'motheaten' fibers, nor inflammatory reactions were observed. We think that (1) changes in skeletal muscles of patients with rheumatoid arthritis and progressive systemic sclerosis may be quite selective, and (2) the myopathy associated with rheumatoid arthritis can be differentiated from inflammatory myopathies, even in muscle biopsy specimens, on the basis of histoenzymologic investigations. more...
- Published
- 1983
28. [Use of the CO2 laser in preneoplastic and neoplastic lesions of the vulva]
- Author
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G, Bandieramonte, M, Regazzoni, S, Andreola, L, Luciani, F, Bianchi, and G, Fava
- Subjects
Adult ,Postoperative Care ,Wound Healing ,Ambulatory Surgical Procedures ,Vulvar Neoplasms ,Humans ,Female ,Laser Therapy ,Carbon Dioxide ,Middle Aged ,Neoplasm Recurrence, Local ,Precancerous Conditions ,Aged - Published
- 1985
29. A multifactorial approach for the prognosis of patients with carcinoma of the stomach after curative resection
- Author
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F, Bozzetti, G, Bonfanti, A, Morabito, R, Bufalino, V, Menotti, S, Andreola, R, Doci, and L, Gennari
- Subjects
Adult ,Male ,Carcinoma ,Middle Aged ,Prognosis ,Gastrectomy ,Stomach Neoplasms ,Humans ,Regression Analysis ,Female ,Neoplasm Invasiveness ,Lymph Nodes ,Aged ,Retrospective Studies - Abstract
This study was done to define the prognostic role of some clinical and pathologic variables in patients with carcinoma of the stomach who underwent a curative subtotal gastrectomy for cancer located at the lower two-thirds of the stomach. An univariate and multivariate analysis, according to Cox's regression model, was retrospectively performed upon 361 patients operated upon at the Istituto Nazionale Tumori of Milan from 1965 to 1979 by a curative subtotal gastrectomy. Data were stored by an IBM 4331 computer. Several factors were taken into consideration: age, sex, site and size of tumor, gross appearance, histologic type, invasion of the gastric wall, nodal status and symptoms. Of six variables selected by the univariate analysis, only four (sex, age, lymph node status and degree of invasion in the gastric wall) were validated by the multivariate evaluation, whereas tumor size and symptoms lost their prognostic relevance. The most important variables were nodal status and the degree of invasion in the gastric wall. The influence of age had a different impact on survival time, depending upon nodal status. In fact, patients with positive nodes who were less than 60 years old had the worst prognosis; the same age group with negative nodes had the best prognosis. Multifactorial analysis, according to the automatic interaction detection procedure, showed that prognosis worsened progressively beginning with female patients with negative nodes at pT1 or pT2 (91.6 per cent five year survival rate), male patients with negative nodes at pT1 or pT2 (76.3 per cent five year survival rate), female patients with negative nodes at pT3 or pT4 (62.4 per cent), male patients with negative nodes at pT3 or pT4 (40.0 per cent), patients more than 60 years old with negative nodes (36.8 per cent) and patients less than 60 years old with positive nodes (20.8 per cent). In our opinion, these parameters should be taken into consideration when stratification of patients as candidates to undergo adjuvant treatment after surgical treatment is planned. more...
- Published
- 1986
30. Endoscopic HpD-Laser Photoradiation Therapy (PRT) of Cancer
- Author
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R. Marchesini, P. Pizzetti, F. Zunino, Elsa Melloni, S. Andreola, Pasquale Spinelli, and V. Mirabile
- Subjects
Hematoporphyrin ,Oncology ,medicine.medical_specialty ,business.industry ,Complete remission ,Cancer ,Cancer cachexia ,medicine.disease ,chemistry.chemical_compound ,chemistry ,Internal medicine ,Fiberoptic endoscope ,Medicine ,Photoradiation Therapy ,business ,Survival rate - Abstract
Efforts are being made all over the world to improve the survival rate and the quality of life of cancer patients. These are the main reasons why new methods of treatment are developed and find their place in the management of the disease. It has been demonstrated for many years that malignant tissue as well as embryonic and traumatized tissues possess selective affinity for porphyrins 1,2 and particularly for an acetic-acid-sulfuric-acid derivative of Hematoporphyrin (HpD) 3. At the same time, endoscopic application possibilities have been identified . More recently, after a large experimental study, Dougherty et al. 5 reported to succeed in the treatment of malignant tumors in humans, and Cortese and Kinsey 6 and Hayata et al. 7 reported clinical endoscopic applications. more...
- Published
- 1984
- Full Text
- View/download PDF
31. Temperature rise in biological tissue during Nd:YAG laser irradiation
- Author
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R. Marchesini, G. Fava, A. Schiroli, Elsa Melloni, S. Andreola, Pasquale Spinelli, and H. Emanuelli
- Subjects
Materials science ,Hot Temperature ,Gas laser ,business.industry ,Stomach ,chemistry.chemical_element ,Dermatology ,Thermographic camera ,Laser ,Neodymium ,law.invention ,Body Temperature ,Optics ,chemistry ,law ,Thermography ,Nd:YAG laser ,Humans ,Surgery ,Irradiation ,Laser Therapy ,business ,Penetration depth - Abstract
Few data are available about temperature distribution in tissue during Nd:YAG laser irradiation. To study the heat distribution that produces tissue coagulation, we used a thermographic camera aimed orthogonally to the laser beam axis to obtain thermal maps. Immediately after surgical resection, specimens of human stomach were irradiated near the resected edge, and the heat emitted sideways was detected by an infrared image system. A magnifying lens mounted on the camera enabled us to obtain 0.1 mm spatial resolution of the isothermic curves. The thermal analysis showed that the maximum depth where the increase in temperature reached 25 degrees C (corresponding to a coagulation temperature of about 60 degrees C) was never greater than 3 mm, irrespective of the power and exposure time used. Moreover, the bidimensional thermal maps showed that the temperature did not decrease in a purely exponential fashion along the beam axis, but reached a maximum at about 1 mm beneath the surface. This fact, which confirms the decrepitation theorem, could explain the explosion inside the tissues observed in surgical application of the Nd:YAG laser. more...
- Published
- 1985
32. Endometrial carcinoma: stage I. A retrospective analysis of 262 patients
- Author
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G, De Palo, R, Kenda, S, Andreola, L, Luciani, R, Musumeci, and F, Rilke
- Subjects
Adult ,Adenocarcinoma ,Middle Aged ,Hysterectomy ,Prognosis ,Uterine Neoplasms ,Carcinoma, Squamous Cell ,Humans ,Female ,Castration ,Neoplasm Recurrence, Local ,Aged ,Neoplasm Staging ,Retrospective Studies - Abstract
From 1969 to 1977, 420 patients with endometrial carcinoma were observed and treated at the National Tumor Institute of Milan. Total abdominal hysterectomy and bilateral salpingo-oophorectomy were performed in 351. After careful clinical and pathologic review, 262 patients were classified as having stage I disease. Further treatment included post-operative radium therapy to the vaginal vault. There were 247 cases with adenocarcinoma, 10 with adenoacanthoma, and 5 with adenosquamous or clear cell carcinoma. Of 257 cases with adenocarcinoma or adenoacanthoma, 63 were grade 1, 161 grade 2, and 33 grade 3. Of the total series, only 41 cases had disease limited to the mucosal surface. The 5-year actuarial survival was 91.4% and the recurrence-free survival was 93.4%. The case material was evaluated according to the risk factors, and results were 1) premenopausal patients had a better prognosis (100% recurrence-free survival versus 92.8% for postmenopausal women, P = .003); 2) length of the uterine cavity was not a significant prognostic factor; 3) myometrial invasion alone was not prognostic but correlated with grade of tumor; 4) the grade of the tumor was an important determinant of recurrence (grade 1 98% recurrence-free survival, grade 2 95%, grade 3 79%). With the described therapy, vaginal recurrences were absent. The recurrences were distant in 20% and local with or without distant metastases in 80%. more...
- Published
- 1982
33. Hematoporphyrin-Derivative and Phototherapy in Extensive Basal-Cell Carcinoma of the Dorsal Skin
- Author
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E. Melloni, S. Di Pietro, G. Fava, H. Emanuelli, C. Andreoli, G. Bandieramonte, S. Andreola, Pasquale Spinelli, F. Zunino, and R. Marchesini
- Subjects
Hematoporphyrin ,Dorsum ,medicine.medical_specialty ,Dye laser ,Energy dose ,business.industry ,medicine.medical_treatment ,medicine.disease ,Laser ,Dermatology ,law.invention ,Radiation therapy ,chemistry.chemical_compound ,chemistry ,law ,medicine ,Basal cell carcinoma ,Irradiation ,Nuclear medicine ,business - Abstract
A 75 year old male patient was diagnosed of multiple “basal cell carcinoma in a surface area of l8 × 21 cm2 of the dorsal skin. Contraindications for surgery and radiation therapy made the patient eligible for phototherapy. After hematoporphyrln-derivative (HpD) administration at the dose of 3 mg/kg, body weight, the entire area of lesions was treated 2 times and in 12 fractionated areas, by using Argon or Dye lasers and different exposure times. Five of the 12 fractions were treated with Argon-ion laser at 100 mW/cm2 average irradiance for 20 min, whereas 7 fractions were treated at the same irradiance with Dye laser for 10 min in one, 15 min in 3 and 20 min for the remaining 3. Energy dose of 60 J/cm2 with Dye laser irradiation and 120 J/cm2 with Argon-ion laser irradiation resulted in similar effectiveness from clinical and histologic standpoints for the studied surface epitheliomas. more...
- Published
- 1984
- Full Text
- View/download PDF
34. [Immunohistological aspects of nephropathy with minimal lesions]
- Author
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G, Barbiano di Belgioioso, G, Busnach, R, Galato, G, Torti, S, Andreola, and M, Surian
- Subjects
Immunoglobulin M ,Kidney Glomerulus ,Humans ,Kidney Diseases ,Complement C3 ,Capillaries - Abstract
Renal biopsies from 32 patients, whose light microscopy showed a pattern of minimal changes nephropathy, have been submitted to immunohistological examination. On the basis of immunofluorescence pattern we separated the cases into three groups: the first group (12 cases) was characterized by absence of any glomerular deposit. The second group (9 cases) showed focal and segmental deposits of IgM and sometimes C3 on capillary walls. In the third group (11 cases) we observed slight deposits of IgM and sometimes C3 "comma like" over glomerular structures in all glomeruli. The site of these last deposits was sometimes mesangium and sometimes the capillary walls. The results of immunohistological examinations gave no information about the pathogenetic mechanisms of minimal changes nephropathy. We observed that the patients enclosed in the second group had a worse clinical prognosis, compared with the other two groups. more...
- Published
- 1977
35. Phenotypic heterogeneity of HLA products on human melanoma cells
- Author
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Donatella Taramelli, S. Andreola, Natale Cascinelli, Giuseppe Fossati, Giorgio Parmiani, and Andrea Anichini
- Subjects
medicine.drug_class ,Immunology ,Cell ,Human leukocyte antigen ,Biology ,Monoclonal antibody ,Antigen ,HLA Antigens ,HLA-DQ Antigens ,Genetics ,medicine ,Humans ,Melanoma ,Genetic heterogeneity ,Histocompatibility Antigens Class II ,Antibodies, Monoclonal ,HLA-DR Antigens ,medicine.disease ,Phenotype ,Clone Cells ,medicine.anatomical_structure ,Cancer research ,Human melanoma - Abstract
SUMMARY The expression of class I and class II HLA antigens has been studied on primary and metastatic human melanomas, and on cell clones derived from the latter. A panel of monoclonal antibodies and flow cytofluorometric analysis were used to evaluate the presence of HLA-A, B, C and -DR, DQ antigens on freshly isolated tumour cells. HLA class I antigens were present on 91% and 93% of primary and metastatic tumours, respectively. Sixty per cent of primary and 50% of metastatic melanomas expressed HLA-DR antigens, whereas 38% and 21% of cases were positive for HLA-DQ. A marked heterogeneity was evident among primary and metastatic lesions for expression of class I and II antigens. Similiar findings were obtained by analysing the phenotype of melanoma clones which indicates that a marked antigenic heterogeneity for class I and II HLA antigens occurs even among clones isolated from short-term cultures of metastatic melanomas. more...
- Published
- 1986
36. Monoclonal Antibodies as Prognostic Indicators of Tumor Progression in Breast Cancer
- Author
-
M. I. Colnaghi, R. Agresti, S. Ménard, M. G. Da Dalt, G. Cattoretti, S. Andreola, G. Di Fronzo, M. Del Vecchio, L. Verderio, N. Cascinelli, M. Greco, and F. Rilke
- Subjects
CA15-3 ,Oncology ,medicine.medical_specialty ,Breast cancer ,Tumor progression ,medicine.drug_class ,business.industry ,Internal medicine ,medicine ,Monoclonal antibody ,medicine.disease ,business - Published
- 1988
- Full Text
- View/download PDF
37. Intra-arterial induction chemotherapy for soft tissue sarcomas
- Author
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A. Garbuglia, S. Fissi, Vittorio Quagliuolo, L. Gennari, Paolo G. Casali, P. Bignami, A. Azzarelli, S. Andreola, and Armando Santoro
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Soft Tissue Neoplasms ,Preoperative care ,Limb Salvage Procedure ,Necrosis ,Preoperative Care ,medicine ,Humans ,Infusions, Intra-Arterial ,Aged ,Chemotherapy ,Ifosfamide ,business.industry ,Induction chemotherapy ,Extremities ,Sarcoma ,Hematology ,Middle Aged ,medicine.disease ,Chemotherapy regimen ,Surgery ,Regimen ,Oncology ,Chemotherapy, Adjuvant ,Doxorubicin ,Female ,business ,medicine.drug - Abstract
Summary One hundred-one patients were treated in our institution with intra-arterial preoperative adriamycin (i.a. ADR) for large soft tissue sarcomas of the extremities, 45% recurrent at entry. Of the 78 high grade evaluable patients 38% had clinical and 45% had pathological responses to chemotherapy. Limb conservative surgery was performed in 80% of cases, with about 10% improvement made possible by the preoperative treatment. The local recurrence rate was 29%, and 50% five-year actuarial survival with a median follow-up of 68 months. These poor results are related to the severe selection of high risk cases. The clinical parameter of response was highly predictive of survival: 63% versus 36% (p=0.01) 5-year survival in the group of responders and non-responders, respectively, whereas the pathological parameter of response was not so predictive. The improved effect of delivering the drug intra-arterially is theoretically expected but not proved in our experience. The major practical effect of this induction chemotherapy is the selection of patients with better prognosis and the possiblity of perform, in some cases, easier limb salvage procedures. The last regimen combining i.a. ADR+i.v. Ifosfamide seems to provide little better but not yet significant results. more...
38. PI3KCA/PTEN deregulation contributes to impaired responses to cetuximab in metastatic colorectal cancer patients.
- Author
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F. Perrone, A. Lampis, M. Orsenigo, M. Di Bartolomeo, A. Gevorgyan, M. Losa, M. Frattini, C. Riva, S. Andreola, E. Bajetta, L. Bertario, E. Leo, M. A. Pierotti, and S. Pilotti
- Subjects
- *
CETUXIMAB , *COLON cancer patients , *METASTASIS , *GENETIC mutation , *EPIDERMAL growth factor , *IMMUNOHISTOCHEMISTRY , *NUCLEOTIDE sequence - Abstract
Background: It has been reported that KRAS mutations (and to a lesser extent KRAS mutations with the BRAF V600E mutation) negatively affect response to anti-epidermal growth factor receptor (EGFR) mAbs in metastatic colorectal cancer (mCRC) patients, while the biological impact of the EGFR pathway represented by PI3K/PTEN/AKT on anti-EGFR treatment is still not clear. Patients and methods: We analysed formalin-fixed samples from a cohort of 32 mCRC patients treated with cetuximab by means of EGFR immunohistochemistry, EGFR and PTEN FISH analysis, and KRAS, BRAF, PI3KCA, and PTEN genomic sequencing. Results: Ten (31%) of 32 patients showed a partial response to cetuximab and 22 (69%) did not [nonresponder (NR)]. EGFR immunophenotype and FISH-based gene status did not predict an anti-EGFR mAb response, whereas KRAS mutations (24%) and PI3K pathway activation, by means of PI3KCA mutations (13%) or PTEN mutation (10%)/loss (13%), were significantly restricted to, respectively, 41% and 37% of NRs. Conclusion: These findings suggested that KRAS mutations and PI3KCA/PTEN deregulation significantly correlate with resistance to cetuximab. In line with this, patients carrying KRAS mutations or with activated PI3K profiles can benefit from targeted treatments only by switching off molecules belonging to the downstream signalling of activated EGFR, such as mammalian target of rapamycin. [ABSTRACT FROM AUTHOR] more...
- Published
- 2009
- Full Text
- View/download PDF
39. Radiofrequency Echographic Multi Spectrometry (REMS) Technology for Bone Health Status Evaluation in Kidney Transplant Recipients.
- Author
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Fassio A, Adami G, Andreola S, Ferraro PM, Pisani P, Lombardi FA, Viapiana O, Rossini M, Caletti C, Gambaro G, Gatti M, and Gatti D
- Abstract
Background : A significant loss in bone density and strength occurs during the post-renal-transplant period with higher susceptibility to fracture. The study aims to compare the performance of the Radiofrequency Echographic Multi Spectrometry (REMS) in the bone mineral density assessment with the conventional dual-energy X-ray absorptiometry (DXA) in a cohort of kidney transplant recipients (KTR). Methods : A cohort of 40 patients underwent both DXA and REMS examinations on the lumbar spine and/or proximal femur. The paired t -test was used to compare DXA and REMS measurements; the chi-square test was used to compare the prevalence of osteoporosis/osteopenia. The agreement between the two techniques was assessed through Spearman's correlation. Results : As expected, most KTR patients were osteopenic or osteoporotic with both REMS and DXA (86.5% and 81% for the femur; 88% and 65% for the lumbar spine p < 0.05). A modest correlation (r = 0.4, p < 0.01) was observed at the lumbar spine between the T-score measured by REMS and DXA. A strong correlation was defined between REMS and DXA in the femoral region (r = 0.7, p < 0.0001). Conclusions : The study demonstrates the exchangeability of the two techniques on the proximal femur in KTR and a higher diagnostic accuracy of REMS at the spine level than DXA. more...
- Published
- 2024
- Full Text
- View/download PDF
40. Could synovial fluid be a useful substrate for the forensic analysis of diatoms?
- Author
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Tambuzzi S, Gentile G, Bailo P, Primavera R, Andreola S, and Zoja R
- Subjects
- Humans, Male, Diatoms isolation & purification, Synovial Fluid chemistry, Drowning diagnosis, Forensic Pathology methods
- Abstract
To date, synovial fluid has not been the subject of targeted analysis as a possible substrate to search for the presence of diatoms in the forensic context of drowning. However, its unique characteristics of production and isolation from the external environment could make it suitable for this purpose, similar to what has already been demonstrated in the literature for vitreous humor. By considering this, synovial fluid was analyzed in a specific case that came to our attention, where the coexisting signs of polytrauma and drowning were documented during autopsy, demonstrating a period of vitality during immersion. After a thin smear of the supernatant was obtained from the centrifugation of the synovial fluid sample, diatoms were successfully detected, consistent with those found in other organs and the water of the canal. The detection of diatoms in the synovial fluid was an objective finding, but its generalizability is limited because this was a pilot application. However, in cases where death by drowning is suspected and the body has multiple areas breached by trauma, the technique of analyzing diatoms in the synovial fluid could have great potential. Therefore, it is appropriate to further explore this technique in order to obtain more forensic evidence in such a setting., (© 2024 American Academy of Forensic Sciences.) more...
- Published
- 2024
- Full Text
- View/download PDF
41. Arterial stiffness and cardiovascular risk in patients with nephrolithiasis: a 10-year prospective study.
- Author
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Comellato G, Caletti C, Giani A, Macchi F, Mazzali G, Faccioli S, Zoico E, Andreola S, Gambaro G, Zamboni M, and Fantin F
- Subjects
- Humans, Male, Middle Aged, Female, Prospective Studies, Adult, Pulse Wave Analysis, Risk Factors, Follow-Up Studies, Heart Disease Risk Factors, Vascular Stiffness physiology, Nephrolithiasis physiopathology, Nephrolithiasis complications, Cardiovascular Diseases physiopathology, Cardiovascular Diseases etiology
- Abstract
Background: Nephrolithiasis is frequently associated with cardiovascular diseases. These conditions present common risk factors: systemic inflammation that promotes oxidative stress leading to arterial wall stiffening may also play a role in plaque formation predisposing to nephrolithiasis., Objectives: The aim of this study was to evaluate arterial stiffness indices at baseline and after a 10-year follow-up, in patients with nephrolithiasis compared with patients without., Methods: A total of 82 patients (37 men; mean age 45 ± 13 years) were enrolled at the Geriatrics and Nephrology Outpatient Clinic: 66 were diagnosed with nephrolithiasis, whereas the control group consisted of 16 individuals. At baseline and after 10 years, they underwent clinical evaluation and arterial stiffness measurement, such as carotid-femoral pulse wave velocity (CF-PWV), by arterial applanation tonometry., Results: At baseline, when compared with the control group, patients with nephrolithiasis showed higher SBP and CF-PWV. After 10 years, patients with nephrolithiasis, but not those without, showed a significant raise in CF-PWV, even after adjustment for age and sex. In a stepwise regression model, with CF-PWV changes during the follow-up as the dependent variable, and age, sex, follow-up years, Δ mean arterial pressure, BMI, hypertension and nephrolithiasis as independent variables, nephrolithiasis was proved to be the only significant predictor of ΔCF-PWV, accounting for 6% of the variance., Conclusion: Our study shows higher baseline CF-PWV and greater increase in ΔCF-PWV within 10 years in individuals with nephrolithiasis than in those without, demonstrating an increased cardiovascular risk for nephrolithiasis patients., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
42. Long-Term Bone Mineral Density Changes in Kidney Transplant Recipients Treated with Denosumab: A Retrospective Study with Nonequivalent Control Group.
- Author
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Fassio A, Andreola S, Gatti D, Pollastri F, Gatti M, Fabbrini P, Gambaro G, Ferraro PM, Caletti C, Rossini M, Viapiana O, Bixio R, and Adami G
- Subjects
- Humans, Female, Male, Middle Aged, Retrospective Studies, Adult, Aged, Osteoporosis drug therapy, Absorptiometry, Photon, Denosumab therapeutic use, Bone Density drug effects, Kidney Transplantation, Bone Density Conservation Agents therapeutic use
- Abstract
Data on the effectiveness of denosumab on osteoporosis after kidney transplantation are limited. We investigated the long-term bone mineral density (BMD) changes in kidney transplant recipients (KTRs) treated with denosumab compared to untreated KTRs. We enrolled KTRs treated with denosumab 60 mg/6 months for 4 years. An untreated group of sex and age-matched KTRs with a 1:1 ratio was included. The primary outcome was BMD changes assessed by Dual-energy X-ray Absorptiometry over 4 years. Data on serum creatinine, alkaline phosphatase (ALP), parathyroid hormone, and 25-hydroxyvitamin D were collected. All patients received oral cholecalciferol and calcium supplementation. 23 denosumab-treated KTRs were enrolled, and 23 untreated KTRs. The median time from transplant to the start of denosumab was 4 years (range 0:24). The denosumab group showed a significant increase from baseline in BMD at the lumbar spine (LS) (9.0 ± 10.7%, p < 0.001), and total hip (TH) (3.8 ± 7.9%, p = 0.041). The untreated group showed a significant decrease at all sites (- 3.0 ± 7%, p = 0.041 at the LS; - 6.3 ± 9.2%, p = 0.003 at the TH; - 6.7 ± 9.3%, p = 0.003 at the FN). The between-group differences in percent BMD changes were statistically significant at all sites. Similar results were found for the respective Z-scores. The ALP serum levels significantly decreased from baseline only in the denosumab group, with a significant between-group difference (p = 0.032). No significant differences in serum creatinine, hypocalcaemic events or acute graft rejection rates were observed. Four years of denosumab therapy were associated with increased BMD in KTRs, while untreated KTRs showed significant BMD losses at all sites., (© 2024. The Author(s).) more...
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- 2024
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43. Correction to: Forensic pilot application of rehydrating solutions on human cadaveric skin: what are the effects on hemorrhagic infiltrates?
- Author
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Tambuzzi S, Gentile G, Raud L, Andreola S, Primavera R, and Zoja R
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- 2024
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44. Forensic pilot application of rehydrating solutions on human cadaveric skin: what are the effects on hemorrhagic infiltrates?
- Author
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Tambuzzi S, Gentile G, Raud L, Andreola S, Primavera R, and Zoja R
- Subjects
- Humans, Pilot Projects, Cadaver, Hemorrhage pathology, Organic Chemicals, Skin pathology, Formaldehyde
- Abstract
The microscopic evaluation of hemorrhagic infiltrates is crucial in forensic diagnostics, but it proves challenging in corificated and mummified cadavers. In these cases, pre-treatment with rehydrating solutions is recommended, although their effects on the hemorrhagic infiltrate are not well understood. In this pilot study, we microscopically investigated the effect of two different rehydrating solutions-Sandison's solution and fabric softener-on well-preserved human cadaveric skin samples taken from areas affected by an ecchymotic lesion, comparing them with direct fixation in formalin. Specifically, we examined the topographic distribution of the hemorrhagic infiltrate in each layer of the skin by assigning a semi-quantitative score, conducted mutual comparisons, and performed statistical analysis. Histologically, compared to direct fixation in formalin, a slight and statistically non-significant reduction in the hemorrhagic infiltrate was observed in samples pre-treated with fabric softener. On the other hand, a more pronounced and statistically significant decrease in scores was observed in samples pre-treated with Sandison's solution. This effect is likely due to the fact that Sandison's solution, due to its components, exerts an osmotic effect, partially inducing osmotic lysis of red blood cells. Overall, extensive areas of hemorrhagic infiltrates were preserved, although to a lesser extent, while smaller foci were markedly reduced, sometimes even disappearing. The findings suggest that Sandison's solution has a detrimental effect on cutaneous hemorrhagic infiltrates, emphasizing the importance of being cautious and conducting dual sampling, using both formalin and a rehydrating solution, for forensic examination of mummified or corificated skin samples., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) more...
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- 2024
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45. Colorimetric Barium Detection of Gunshot Residues on Cadaveric Human Skin: A Pilot Application for Forensic Purposes.
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Tambuzzi S, Gentile G, Boracchi M, Andreola S, and Zoja R
- Abstract
Introduction: In this study we microscopically investigated, for the first time ever, the colorimetric detectability of barium of gunshot residues (GSR) on cadaveric human skin with gunshot wounds., Methods: For this purpose we used two different colorimetric techniques known in the literature, namely 0.2% sodium rhodizonate (Na-R-Ba 0.2%) and sodium rhodizonate in alcoholic environment (Na-R-Ba OH 0.2%). At the same time, we have also coupled it with scanning electron microscopy with energy dispersive X-ray (SEM/EDX) analysis and the colorimetric study for the detection of lead of GSR. These techniques were applied to 16 victims who died from gunshot injuries, as well as to a control group., Results: SEM/EDX demonstrated the presence of lead in all cases and barium in 11 of the 16 cases. The subsequent colorimetric technique with Na-R-Ba 0.2% did not show the barium of GSR in any case, unlike the Na-R-Ba OH 0.2% technique. This latter, in fact, has demonstrated the presence of this metal in 2 cases (18%). No microscopic case of false positive was recorded., Conclusion: The evidence obtained with Na-R-Ba OH 0.2% makes this method, applied here for the first time ever, worthy of further study. Meanwhile, although this technique can certainly be applied, it cannot be separated from the contextual colorimetric investigation for lead and the use of more sophisticated techniques., Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2024.) more...
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- 2024
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46. Post-mortem diagnosis of amniotic fluid embolism.
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Gentilomo A, Tambuzzi S, Gentile G, Boracchi M, Andreola S, and Zoia R
- Abstract
Competing Interests: Conflict of interest: None.
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- 2024
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47. Fatal pulmonary thromboembolism associated with uterine carcinosarcoma.
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Lodetti G, Tambuzzi S, Andreola S, Gentile G, and Zoja R
- Abstract
Competing Interests: Conflict of interest: None.
- Published
- 2023
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48. Elastic fiber degradation as a possible indicator for PMI estimation on mummified and corified skin: a pilot study.
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Tambuzzi S, Gentile G, Andreola S, Bilardo G, Collini F, Bailo P, and Zoja R
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- Humans, Pilot Projects, Autopsy, Staining and Labeling, Postmortem Changes, Elastic Tissue, Burial
- Abstract
Mummified and corified bodies are particularly complex scenarios to investigate, starting from identifying the post-mortem interval (PMI), even more so in indoor environments. In these bodies, the skin has the peculiar feature to resist for a long time. Among its components, there are elastic fibers, which are characterized by intrinsic resistance to post-mortem degenerative phenomena. Starting from these considerations, we investigated microscopically the persistence, detectability, and changes of elastic fibers in the skin of mummified and corified bodies with different known PMI. The aim was to evaluate whether they could provide an additional tool to aid in PMI estimation in these cases. Therefore, we collected skin samples from mummified or corified bodies found in a domestic environment with different known PMI, as well as from corified bodies that had been exhumed after 11 years of burial. Histochemical staining specific for elastic fibers, namely, Weigert's resorcin fuchsin, showed their prolonged persistence and a progressive and different degradation between mummified and corified skin as a function of PMI. Moreover, on the whole, we observed greater preservation of elastic fibers in mummified skin than in corified one at the same PMI. Therefore, histological analysis of elastic fibers in mummified and corified skin may help to provide valuable aid in estimating PMI, especially in those particular cases where more reliable alternatives are lacking., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) more...
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- 2023
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49. Association analysis of 10 candidate genes causing Mendelian calcium nephrolithiasis in the INCIPE study: a South European general population cohort.
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Santoro G, Lombardi G, Andreola S, Salvagno GL, Treccani M, Locatelli E, Ferraro PM, Lippi G, Malerba G, and Gambaro G
- Abstract
Background: Idiopathic calcium nephrolithiasis (ICN) is a common condition with a complex phenotype influenced by both environmental and genetic factors. In our study we investigated the association of allelic variants with the history of nephrolithiasis., Methods: We genotyped and selected 10 candidate genes potentially related to ICN from 3046 subjects participating in the INCIPE survey cohort (Initiative on Nephropathy, of relevance to public health, which is Chronic, possibly in its Initial stages, and carries a Potential risk of major clinical End-points), a study enrolling subjects from the general population in the Veneto region in Italy., Results: Overall, 66 224 variants mapping on the 10 candidate genes were studied. A total of 69 and 18 variants in INCIPE-1 and INCIPE-2, respectively, were significantly associated with stone history (SH). Only two variants, rs36106327 (chr20:54 171 755, intron variant) and rs35792925 (chr20:54 173 157, intron variant) of the CYP24A1 gene were observed to be consistently associated with ICN. Neither variant has been previously reported in association with renal stones or other conditions. Carriers of CYP24A1 variants showed a significant increase in the ratio of 1,25 (OH)
2 vitamin D to 25 (OH) vitamin D compared with controls ( P = .043). Although not associated with ICN in this study, the rs4811494 CYP24A1 variant that was reported to be causative of nephrolithiasis was very prevalent in heterozygosity (20%)., Conclusion: Our data suggest a possible role for CYP24A1 variants in the risk of nephrolithiasis. Genetic validation studies in larger sample sets will be necessary to confirm our findings., (© The Author(s) 2023. Published by Oxford University Press on behalf of the ERA.) more...- Published
- 2023
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50. Utility and diagnostic value of postmortem microbiology associated with histology for forensic purposes.
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Tambuzzi S, Maciocco F, Gentile G, Boracchi M, Faraone C, Andreola S, and Zoja R
- Subjects
- Humans, Forensic Medicine, Autopsy methods, Cadaver, Forensic Pathology, Forensic Microbiology, Infections
- Abstract
Nowadays, the diagnostic value of postmortem microbiological investigations is still a debated topic, but postmortem microbiology (PMM) remains a discipline with great forensic potential. To evaluate the usefulness and diagnostic-forensic value of postmortem microbiological cultures, it has been conducted a study on cadaveric material sampled during autopsy aiming to identify the correct cause of death. The study analyzed 45 cadavers subjected to judicial autopsy, divided into two groups based on the presence or absence of external or internal macroscopic autopsy signs suggesting infectious pathology. In the same cases, both the microbiological and conventional histological investigations have been simultaneously carried out. From the investigations, mono-bacterial, mono-fungal, mixed and negative cultures were observed. In mono-species microbiological growth, the histological epicrisis confirmed an infectious cause of death due to the presence of signs of acute infection with an aggressive infectious agent. In cases where growth was mixed, it was possible to distinguish between simple postmortal contamination and perimortem colonization. Finally, in some cases where the microbiology was negative, this has been essential in highlighting signs of a vital reaction to viral or parasitic infection. The joint and integrated evaluation of the laboratory results made it possible to correctly understand even those peculiar situations in which the PMM results alone would not have been significant. These methods, when combined, constitute an optimal forensic approach for the identification of the real cause of death and thus reduce the number of unsolved cases., Competing Interests: Conflicts of interest/Competing interests The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (Copyright © 2022 Elsevier B.V. All rights reserved.) more...
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- 2023
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