Search

The effects of clinoptilolite on milk copper (Cu), lead (Pb), zinc (Zn), cadmium (Cd) and iron (Fe) concentrations, proinflammatory cytokine responses, oxidative stress status, whole blood cell counts and liver and kidney functions were investigated in dairy cows exhibiting no signs of any kind of toxicity. Clinoptilolite was added to the feed at a dose of 200 mg kg−1 body weight in the clinoptilolite-treated group (n = 14), but was not added to the feed in the control group (n = 7). In the milk samples (n = 21) collected before the experiment, the Cu, Pb, Zn, Cd and Fe values were 0.021 ± 0.020, 0.104 ± 0.01, 3.42 ± 0.32

Dilated cardiomyopathy (DCM) is defined as a cardiac disease characterized by dilatation of the cardiac chamber, decreased myocardial contractility, and decreased left ventricular ejection fraction. In this study, we aimed to determine whether there is a tendency to age-related dilated cardiomyopathy in Kangal Dogs, a breed of Anatolia, echocardiographically. The experimental groups of the study consisted of a total of 20 dogs, 10 of which were 1-3 years old, young group (Group I) and the other 10 were 4-7 years old, middle-aged group (Group II), who had no clinical problems at rest. By echocardiographic examination, the end-diastolic thickness of interventricular septum, the end-systolic thickness of interventricular septum, left atrial diastolic diameter, aortic root diastolic inner diameter, the ratio of left atrium diameter to aortic diameter, left ventricular end-diastolic inner diameter, left ventricular end-systolic inner diameter end-systolic thickness of the posterior wall, the end-diastolic thickness of the posterior wall of the left ventricle, heart rate, left ventricular ejection fraction, left ventricular contraction force, stroke volume, cardiac output, end-diastolic volume and end-systolic volume parameters were measured. The data collected from the two groups were analysed using independent samples T-test. In conclusion, considering that the age of the Kangal dogs constituting the middle-aged group (G II) of the study was within the age limits specified in the studies (6-7 years), a myocardial defect indicating echocardiographic DCM was not detected. Thus, it was concluded that there is no tendency for DCM among the age groups included in the study. However, it can be said that echocardiographic evaluation of more coiled dogs in the given age group may give a more reliable result considering that the rate of DCM is 6% in different breed dogs.

Aim: This study aimed to investigate the therapeutic efficacy of thyme extract in puppies naturally infected with ascarids. Methodology: The study consisted of 20 puppies of different sexes (males and females), 2-4 months old, naturally infected with ascarid. The experimental units were given an oral 20% concentration of thyme extract for 3 days, and faecal oocyst counts were conducted on the 0th, 1st, 2nd, 3rd, and 7th days starting from the day the treatment was started (day 0). Also, urea, creatinine, AST, ALT values in the blood were monitored on the 0th and 3rd days, together with daily clinical examination, to monitor possible toxic effects. Results: While the number of faecal egg counts in 2 puppies (10%) varied considerably, egg shedding in 8 (40%) of the treated puppies was zero. It was observed that in 10 of the puppies (60%), egg shedding was not completely zero. However, the oocyst counts decreased by 25% to 98.3%. It was also observed that the values of the measured blood biochemical values were within reference range and the puppies did no not show any clinical sign of toxicity during treatment. Conclusion and Recommendation: It was concluded that the thyme extract did not have any toxic effect in the puppies at the concentration studied, and it could be effective in the treatment of ascariasis.

This study aimed to determine the presence of Mycoplasma haemofelis (Mhf), which is a member of the hemotropic mycoplasmas called infectious anemia of cats, known to be zoonotic, usually progresses with hemolytic anemia in cats, and can lead to the death of the cat if not treated, in the Kırıkkale province by the Polymerase Chain Reaction (PCR) method. The experimental units of the study consisted of 50 male and 50 female cats of different breeds, aged between 2-months and 10-years, presented to the Kırıkkale University, Faculty of Veterinary Medicine, Internal Medicine, Surgery and Obstetrics Clinics with different complaints during January-August 2021. Smears prepared from blood samples were examined cytologically by the Giemsa. In addition, total blood count was done and the increased blood samples were analysed by PCR for the 16S ribosomal RNA gene. According to the PCR analysis findings of the study, it was concluded that the incidence of Mycoplasma haemofelis in Kırıkkale is 13 %.

In this study, the prophylactic and therapeutic activities of thyme extract at different concentrations against experimental Cryptosporidium parvum infection in immunosuppressed rats were investigated. Thyme extract was prepared at four different concentrations (10%, 30%, 50%, and 100%) and administered as a single oral dose of 1 mL for evaluation of its prophylactic efficacy. Five consecutive days after infection was detected in all rats, therapeutic evaluations were also performed. According to the results obtained by daily counting of oocysts in stools, the prophylactic and therapeutic effects of thyme extract administration were significant in comparison to the control group (P˂0.01). Oocyst shedding continued in the control group at high numbers from the beginning to the end of the study, while oocyst counts in the prophylaxis groups remained low throughout the study. On the other hand, oocyst excretion rates were high in the therapeutic groups and decreased rapidly after thyme extract administration. At the end of the study, oocyst excretion had completely stopped for some rats administered thyme extract. There was no group in which oocyst shedding ceased for all rats. No significant differences were observed in the therapeutic or prophylaxis groups regarding the doses administered (P 0.01). Renal and hepatic functions were monitored by measuring urea, creatinine, alanine transaminase, and aspartate transaminase levels ​​before and after thyme extract administration. As a result, it was concluded that oral thyme extract administration at the doses applied in this study is effective and safe in the prophylactic and therapeutic treatment of experimental cryptosporidiosis in rats.

Feng, Yaoyu/0000-0002-8380-1246; Ozkan, Aysegul Taylan/0000-0001-8421-3625; Feng, Yaoyu/0000-0002-8380-1246; Xiao, Lihua/0000-0001-8532-2727; TAYLAN-OZKAN, Aysegul/0000-0001-8421-3625 WOS: 000388059400003 PubMed: 27373430 Molecular characterizations of Cryptosporidium spp. in ruminants reared under traditional animal management systems are scarce and studies conducted thus far have revealed largely an absence of the pathogenic and zoonotic species Cryptosporidium parvum in pre-weaned animals. In this study, we examined Cryptosporidium species and subtype distribution in free-range pre-weaned dairy calves and goat kids with diarrhea. Cryptosporidium-positive specimens from pre-weaned calves on 10 farms and goat kids on 4 farms in Ankara, Balikesir, Corum, Kirikkale, and Kirsehir Provinces, Turkey were genotyped by PCR-restriction length polymorphism analysis of the small subunit rRNA gene, which identified C. parvum in 27 calves and 9 goat kids and Cryptosporidium ryanae in 1 calf. Among the C. parvum isolates successfully subtyped by DNA sequence analysis of the 60 kDa glycoprotein gene, three subtypes were detected in calves, including IlaA13G2R1 (20/23), IldA18G1 (2/23), and IldA20G1b (1/23), and four subtypes were detected in goat kids, including IlaA13G2R1 (3/8), IlaA15G1R1 (2/8), IldA22G1 (2/8), and IldA18G1 (1/8). Data of the study suggest that dairy calves reared in a traditional cow-calf system in Turkey are mainly infected with a C. parvum subtype rarely seen elsewhere, whereas goat kids are infected with diverse subtypes. As all five C. parvum subtypes found in this study are known human pathogens, pre-weaned farm animals could play a potential role in the transmission of human cryptosporidiosis. (C) 2016 Elsevier Inc. All rights reserved. National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31229005, 31110103901]; Centers for Disease Control and PreventionUnited States Department of Health & Human ServicesCenters for Disease Control & Prevention - USA This research was supported by the National Natural Science Foundation of China (Project No. 31229005 and 31110103901) and Centers for Disease Control and Prevention.

YILDIZ, Kader/0000-0001-5802-6156 WOS: 000292339200008 PubMed: 21726229 Four cats were presented with respiratory signs and first-stage larvae of Aelurostrongylus abstrusus were found in faecal samples. Anthelmintic treatment was given to the infected cats and venous blood gases were analysed during the treatment period. Blood gas analysis suggested hypoventilation and respiratory acidosis in infected cats. Hypoventilation may be the result of airway obstruction by adults and larvae in respiratory bronchioles and the alveolar canals. The blood gas values had returned close to the physiological range by two months after treatment. Assessment of respiratory acidosis may aid development of additional treatment methods in cats infected with A. abstrusus.

Kopeklerde alerjik kontak dermatitis (AKD) vakalarina pratikte rastlanmakla birlikte, gercek insidansinin belirlenmesi oldukca zordur. Yama testi, kontakt alerjenlere bagli olarak gelisen ekzematoz tipte deri rahatsizliklarinin tanisina yonelik, fakat henuz kopeklerde standardizasyonu tamamlanmamis, invaziv olmayan bir testtir. Bu arastirmada, insan hekimligi icin standardize edilmis yama testi yontemi kullanilarak dermatitisli kopeklerde gorulen alerjenlerin cesitleri ve bunlarin klinik tablo ile iliskisi arastirilmistir. Besledigi hayvana karsi duyarlilik, atopik veya astimli insanlarda sik gorulen alerji semptomlaridir. Bu nedenle, calismanin ikinci asamasinda kopek sahiplerinde kopeklerine karsi bir alerjik duyarlilik gelisip gelismedigi de arastirilmistir. Calismada 22 adet alerjik dermatitisli kopege, standart “Avrupa yama testi” uygulandi. Yama testi 9 (%41) kopekte pozitif sonuc verirken, pozitif alerjenler kapsaminda nikel sulfat, potasyum dikromat, cinko-diethydithiokarbamat, p-t-butylph.-formaldesid, “fragrance“ karisimi, IPPD, benzokain, civa (II)-amid-klorid, merkapto karisimi, setylstearylalkohol saptandi. Kopek sahiplerinin (n = 12) yanisira kontrol olarak kopek sahibi olmayan kisilerin (n = 10), kan numunelerinde spesifik IgE (kopek epiteli ve kepegi) floroenzimimmunoassay calisildi. Kopek sahiplerinde herhangi bir alerji belirtisi gozlenmemis olmasina ragmen, hepsi spesifik IgE’ye karsi pozitif bulundu. Diger yanda kontrol grubunda, yalnizca 4 kisinin (40%) spesifik IgE acisindan pozitif oldugu anlasildi. Bu calismanin verileri, kopek antijeninden tamamen ari olmanin mumkun olmayabilecegini gostermektedir. Ayrica, bu bulgular atopik dermatitli kopeklerde, kontakt alerjenlerin potansiyel varligini desteklemektedir

Bu raporda Bolu yöresindeki bir sığır sürüsündetespit edilen Botulismus vakalarında klinik, hematolojik ve biyokimyasaldeğişikliklerin belirlenmesi amaçlanmıştır.Bu amaçla çalışmanın materyalini salgın sonucunda sağ kalan 10 sığıroluşturdu ve bu sığırların klinik, hematolojik ve biyokimyasal muayeneleriyapıldı. Anamnezde yetersiz mera koşulları nedeni ile tavuk gübresi ve tavukleşleri ile kontamine olmuş arazide otlayan sığırlarda 3 gün sonra ilksemptomların ortaya çıktığı belirlendi. Klinik semptom olarak; sallantılıyürüyüş, kas tremoru, dilin dışarı çıkması, kuyrukta paraliz, costo-sternalyatma, ön ve arka bacaklarda paraliz belirlendi. Hematolojik muayenede bütünparametrelerin referans değerler arasında olduğu tespit edildi. Biyokimyasalincelemelerde serum kolesterol ve aspartat aminotransferaz (AST) değerleriyüksek bulunurken bakır (Cu), demir (Fe), fosfor (P), mangan (Mn) ve selenyum(Se) değerlerinin referans değerlerden düşük olduğu saptandı. Klinik olarakBotulismus tanısı konulan sığırlarda hematolojik parametrelerde değişiklikolmadığı belirlendi. Botulismustankorunmak ve kontrol etmek için; toksin kaynağının engellenmesi ve yok edilmesi,mera ıslahı, mineral takviyesi ve kadavraların uygun şekilde yok edilmesi ileaşılama gibi tedbirlerin uygulanması gerektiği kanısına varıldı.

Çalışmada dışkılarında Cryptosporidium parvum ookistleri tespit edilen 23 (18 buzağı tedavi grubu, 5 buzağı kontrol grubu) ishalli buzağıda tylosinenin (Tylan 200, Elanco) terapötik etkinliği incelenmiştir. Tedavi grubundaki buzağılara günde bir kere 20 mg/kg dozunda tylosin 5 gün süreyle intramuskuler olarak uygulandı. Dört buzağıda tedavinin dördüncü, 8 buzağıda altıncı, 6 buzağıda yedinci gününden itibaren dışkıda ookiste rastlanmadı. Kontrol grubunda ise 10 gün süresince incelenen dışkılarında ookist sayısında önemli bir azalma görülmedi. Çalışmada dışkıdaki ookist sayılarına bakılarak tylosinenin günde bir kere 20 mg/kg dozunda 5 gün süreyle intramuskuler olarak uygulanması buzağı kriptosporidiozunun tedavisinde etkili bulundu. In der vorliegenden Arbeit wurde auf 23 (18 Kälber Therapiegruppe, 5 Kälber Kontrollgruppe) durchfällige, in deren Kot Cryptosporidium parvum oozysten nachgewiesene Kälbern die therapeutische Wirksamkeit des Tylosins (Tylan® 200, Elanco) geprüft. Die Kälber der Therapiegruppe wurden einmal täglich, 5 Tage lang, in einer Dosierung von 20 mg/kg/Tag, Intramuskulär, mit Tylosin behandelt. Im Kot von 4 Kälbern konnten ab 4. Tag, bei 8 Kälbern ab 6. Tag und bei 6 Kälbern ab 7. Tag nach Behandlungsbeginn keine Oozysten mehr nachgewiesen werden. Bei der Kontrollgruppe wurde die Oozysten im Kot bis 10 Tagen untersucht und wurde keine wichtige verminderung bei der Oozystenzahlen beobachtet. In dieser Arbeit unter Berücksichtigung der Oozystzahlen im Kot wurde Tylosin in einer Dosierung von 20 mg/kg, einmal täglich, für die Dauer von 5 Tagen Intramuskulär beim Bekämpfung der Kälberkryptosporidiose wirkungsvoll gefunden.

This study was carried out in order to compare the seroprevalence of visceral leishmaniasis in dogs from different areas of Kirikkale between 2006-2008 using the microculture method (MCM) which is a new method, and the indirect fluorescent antigen test (IFAT). All of the blood collected from total of 50 dogs was found to be negative by MCM. Only one male Beagle strain dog (3 years old) was found to be seropositive at 1/128 titers (2%) for anti-Leishmania infantum IgG antibodies by IFAT.

Heartworm infection is one of the most important parasitic diseases in dogs. The aim of the present study was to determine the prevalence of heartworm in dogs in Kirikkale. Blood samples of 172 dogs were collected. Microfilariae were detected in 10 (5.8%). dogs with the Knott technique. The anal and excretory pores stained with acid phosphates showed that the microfilariae belonged to Dirofilaria immitis. Microfilariae were found in dogs aged between 2-10 years, and especially in Siberian huskies, setters, pointers and crossbred sheepdogs. The serum samples of 142 dogs were analyzed using the commercial ELISA test kit for detection of anti-D. immitis antibody. The occult infection rate was found to be 27.46% in dogs. According to these results, Kirikkale region has a high risk for heartworm infection.

WOS: 000258776500014 This communication aims to report cholelithiasis diagnosed by radiography, ultrasonography and necropsy in a 6 month old Anatolian Shepherd dog. Clinical signs and laboratory findings also revealed distemper besides cholelithiasis and therefore the case had to be euthanized. The diagnosis was confirmed by necropsy. Cholelithiasis is commonly reported in adult and old dogs but not in young therefore the case was found to be worth publishing.

Sunulan çalışmanın amacı kedilerin enfeksiyöz anemisi olarak adlandırılan, zoonoz olduğu bilinen, kedilerde genellikle hemolitik anemi ile seyreden doğru tedavi yapılmazsa kedinin ölümüne yol açabilen hemotropik mikoplazmaların bir üyesi olan Mycoplasma haemofelis'in Kırıkkale ilinde varlığının PCR metodu ile belirlenmesidir. Çalışmanın materyalini Kırıkkale Üniversitesi Veteriner Fakültesi Dahiliye, Cerrahi ve Doğum Kliniklerine 2021 Ocak – Ağustos aylarında farklı şikayetlerle getirilen, yaşları 2 ay-10 yaş arasında değişen, farklı ırklardan, 50 erkek, 50 dişi kedi oluşturdu. Ayrıntılı anamnezleri alınıp, klinik muayeneleri yapılan kedilerden alınan kan örneklerinden hazırlanan frotiler Giemsa boyama yöntemi ile sitolojik olarak incelendi. Ayrıca kanlardan tam kan sayımı yapılarak, artan kan örnekleri 16S ribozomal RNA geninin PCR analizine tabi tutularak incelendi. Sonuç olarak; PCR analiz bulgularına göre Kırıkkale'de Mycoplasma haemofelis insidensi %13 olarak belirlendi. This study aimed to determine the presence of Mycoplasma haemofelis, which is a member of the hemotropic mycoplasmas called infectious anemia of cats, known to be zoonotic, usually progresses with hemolytic anemia in cats, and can lead to the death of the cat if it is not treated, in the Kırıkkale province by the Polymerase Chain Reaction (PCR) method. The experimental units of the study consisted of 50 male and 50 female cats of different breeds, aged between 2-months and 10-years, brought to the Kırıkkale University Faculty of Veterinary Medicine, Internal Medicine, Surgery and Obstetrics Clinics with different complaints during January-August 2021. Scratches (Smears) prepared from blood samples taken from cats whose detailed anamnesis were taken and clinically examined and cytologically examination were performed by the Giemsa staining method. In addition, total blood count was done and the increased blood samples (blood samples with higher counts) were analysed by PCR for the 16S ribosomal RNA gene. According to the PCR analysis findings of the study, it was concluded that the incidence of Mycoplasma haemofelis in Kırıkkale is 13 %.