Effects of clinoptilolite on heavy metal levels in milk, proinflammatory cytokine responses (IL-1β and IL-6) and oxidative stress in dairy cows

The effects of clinoptilolite on milk copper (Cu), lead (Pb), zinc (Zn), cadmium (Cd) and iron (Fe) concentrations, proinflammatory cytokine responses, oxidative stress status, whole blood cell counts and liver and kidney functions were investigated in dairy cows exhibiting no signs of any kind of toxicity. Clinoptilolite was added to the feed at a dose of 200 mg kg−1 body weight in the clinoptilolite-treated group (n = 14), but was not added to the feed in the control group (n = 7). In the milk samples (n = 21) collected before the experiment, the Cu, Pb, Zn, Cd and Fe values were 0.021 ± 0.020, 0.104 ± 0.01, 3.42 ± 0.32