Search

Abstract AMoRE-II aims to search for neutrinoless double beta decay ( $$0\nu \beta \beta $$ 0 ν β β ) with an array of 423 $$\hbox {Li}_2^{100}\hbox {MoO}_4$$ Li 2 100 MoO 4 crystals operating in the cryogenic system as the main phase of the Advanced Molybdenum-based Rare process Experiment (AMoRE). AMoRE has been planned to operate in three phases: AMoRE-pilot, AMoRE-I, and AMoRE-II. AMoRE-II is currently being installed at the Yemi Underground Laboratory, located approximately 1000 m deep in Jeongseon, Korea. The goal of the experiment is to reach an exclusion half-life sensitivity to the $$0\nu \beta \beta $$ 0 ν β β of $$^{100}$$ 100 Mo on the level of $$T^{0\nu \beta \beta }_{1/2} > 6 \times 10^{26}$$ T 1 / 2 0 ν β β > 6 × 10 26 year that covers completely the inverted Majorana neutrino mass hierarchy region of (15–46) meV. To achieve this, the background level of the experimental configurations and possible background sources of gamma and beta events should be well understood. We have intensively performed Monte Carlo simulations using the GEANT4 toolkit in all the experimental configurations with potential sources. We report the estimated background level that meets the $$10^{-4}$$ 10 - 4 counts/(keV $$\cdot $$ · kg $$\cdot $$ · year) requirement for AMoRE-II in the Region Of Interest (ROI) and show the projected half-life sensitivity based on the simulation study.

Abstract The wisent (Bison bonasus) is Europe’s largest land mammal. We produced a HiFi read-based wisent assembly with a contig N50 value of 91 Mb containing 99.7% of the highly conserved single copy mammalian genes which improves contiguity a thousand-fold over an existing assembly. Extended runs of homozygosity in the wisent genome compromised the separation of the HiFi reads into parental-specific read sets, which resulted in inferior haplotype assemblies. A bovine super-pangenome built with assemblies from wisent, bison, gaur, yak, taurine and indicine cattle identified a 1580 bp deletion removing the protein-coding sequence of THRSP encoding thyroid hormone-responsive protein from the wisent and bison genomes. Analysis of 725 sequenced samples across the Bovinae subfamily showed that the deletion is fixed in both Bison species but absent in Bos and Bubalus. The THRSP transcript is abundant in adipose, fat, liver, muscle, and mammary gland tissue of Bos and Bubalus, but absent in bison. This indicates that the deletion likely inactivates THRSP in bison. We show that super-pangenomes can reveal potentially trait-associated variation across phylogenies, but also demonstrate that haplotype assemblies from species that went through population bottlenecks warrant scrutiny, as they may have accumulated long runs of homozygosity that complicate phasing.

Abstract Background Association testing between molecular phenotypes and genomic variants can help to understand how genotype affects phenotype. RNA sequencing provides access to molecular phenotypes such as gene expression and alternative splicing while DNA sequencing or microarray genotyping are the prevailing options to obtain genomic variants. Results We genotype variants for 74 male Braunvieh cattle from both DNA (~ 13-fold coverage) and deep total RNA sequencing from testis, vas deferens, and epididymis tissue (~ 250 million reads per tissue). We show that RNA sequencing can be used to identify approximately 40% of variants (7–10 million) called from DNA sequencing, with over 80% precision. Within highly expressed coding regions, over 92% of expected variants were called with nearly 98% precision. Allele-specific expression and putative post-transcriptional modifications negatively impact variant genotyping accuracy from RNA sequencing and contribute to RNA-DNA differences. Variants called from RNA sequencing detect roughly 75% of eGenes identified using variants called from DNA sequencing, demonstrating a nearly 2-fold enrichment of eQTL variants. We observe a moderate-to-strong correlation in nominal association p-values (Spearman ρ2 ~ 0.6), although only 9% of eGenes have the same top associated variant. Conclusions We find hundreds of thousands of RNA-DNA differences in variants called from RNA and DNA sequencing on the same individuals. We identify several highly significant eQTL when using RNA sequencing variant genotypes which are not found with DNA sequencing variant genotypes, suggesting that using RNA sequencing variant genotypes for association testing results in an increased number of false positives. Our findings demonstrate that caution must be exercised beyond filtering for variant quality or imputation accuracy when analysing or imputing variants called from RNA sequencing.

Accurate volcanic hazard assessments rely on a detailed understanding of the timing of past eruptions. While radiometric methods like 40Ar/39Ar or K/Ar are by far the most conventional lava flow dating tools, their low resolution for young ( ka) deposits interferes with the development of precise chronologies of recent effusive activity on most volcanoes. Mt Ruapehu (Aotearoa / New Zealand) has produced many lava flows throughout its history, but the precise timing of many recent eruptions remains largely unknown. In this study, we use cosmogenic 3He exposure dating to provide 23 eruption ages of young lava flows at Ruapehu. We then compare our results with existing 40Ar/39Ar and paleomagnetic constraints, highlighting the value of cosmogenic nuclide exposure dating in refining recent eruptive chronologies. Of the 23 sampled flows, 16 provided robust eruption ages (5 %–20 % internal 2σ; n≥3) between ca. 20 and 8 ka, except for one lava flow that erupted at around 43 ka, and their age distribution indicates that, during the last 20 kyr, effusive activity at Ruapehu peaked at 17–12 ka and at 9–7.5 ka. Nearly identical eruption ages of lavas located in different flanks of the volcanic edifice suggest concurrent activity from multiple vents during relatively short time intervals (0–2 kyr) at around 13, 10, and 8 ka. We analysed four individual lava flows previously dated by 40Ar/39Ar, two of which yield eruption ages older than the older limit of the 2σ interval of the radiometric dates, but the good clustering of individual samples from our sites suggests that our results better represent the real eruption age of these flows. Our 3He-based chronology shows excellent agreement with paleomagnetic constraints, suggesting that production rate uncertainties are unlikely to impact the accuracy of our eruption ages. This study demonstrates how cosmogenic nuclide dating can provide greater detail on the recent effusive chronology of stratovolcanoes, helping to resolve the low resolution of and difficulty in applying radiometric dating methods to young lava flows.

Abstract We present a comprehensive study of the nonproportionality of NaI(Tl) scintillation detectors within the context of dark matter search experiments. Our investigation, which integrates COSINE-100 data with supplementary $$\gamma $$ γ spectroscopy, measures light yields across diverse energy levels from full-energy $$\gamma $$ γ peaks produced by the decays of various isotopes. These $$\gamma $$ γ peaks of interest were produced by decays supported by both long and short-lived isotopes. Analyzing peaks from decays supported only by short-lived isotopes presented a unique challenge due to their limited statistics and overlapping energies, which was overcome by long-term data collection and a time-dependent analysis. A key achievement is the direct measurement of the 0.87 keV light yield, resulting from the cascade following electron capture decay of $$\mathrm {^{22}Na}$$ 22 Na from internal contamination. This measurement, previously accessible only indirectly, deepens our understanding of NaI(Tl) scintillator behavior in the region of interest for dark matter searches. This study holds substantial implications for background modeling and the interpretation of dark matter signals in NaI(Tl) experiments.

Abstract Dating young lava flows is essential for understanding volcano's eruption frequency, yet challenging due to methodological limitations of commonly used dating techniques. Ruapehu (Aotearoa New Zealand) produced many lava flows during the Holocene, but constraints on the timing of these eruptions are scarce. Here, we use paleomagnetic dating to deliver new eruption ages of 18 lava flows with uncertainties ranging between 500 and 2,700 years (at the 95% confidence level). Comparison between lava flows' paleomagnetic directions and a local paleosecular variation record indicates that the large lava flow field located on the Whakapapa area was emplaced during at least three distinct eruptive episodes between 10600 and 7400 BP. Two of these episodes closely followed a large collapse event that affected Ruapehu's northern area and generated large volumes of lava between 10600 and 8800 BP, with the third episode producing less voluminous lava flows between 8100 and 7400 BP. Following a smaller collapse of the southeastern sector of the edifice at ca. 5300 BP, several low‐volume lava flows were emplaced during at least two distinct eruptive episodes prior to ca. 1000 BP, which supplied the Whangaehu valley with lava. The youngest age inferred from our data represents the youngest eruption age provided for a lava flow outside Ruapehu's summit region. This research provides greater detail to the Holocene effusive chronology at Ruapehu, shedding light on partial cone reconstructions after edifice collapses during the Holocene, and the time relationships between trends observed in its effusive and explosive activity.

Neutrinoless double beta decay is one of the most sensitive probes for new physics beyond the Standard Model of particle physics. One of the isotopes under investigation is ^{136}Xe, which would double beta decay into ^{136}Ba. Detecting the single ^{136}Ba daughter provides a sort of ultimate tool in the discrimination against backgrounds. Previous work demonstrated the ability to perform single atom imaging of Ba atoms in a single-vacancy site of a solid xenon matrix. In this paper, the effort to identify signal from individual barium atoms is extended to Ba atoms in a hexa-vacancy site in the matrix and is achieved despite increased photobleaching in this site. Abrupt fluorescence turn-off of a single Ba atom is also observed. Significant recovery of fluorescence signal lost through photobleaching is demonstrated upon annealing of Ba deposits in the Xe ice. Following annealing, it is observed that Ba atoms in the hexa-vacancy site exhibit antibleaching while Ba atoms in the tetra-vacancy site exhibit bleaching. This may be evidence for a matrix site transfer upon laser excitation. Our findings offer a path of continued research toward tagging of Ba daughters in all significant sites in solid xenon.

The AMoRE-II experiment will search for the 0νββ decay of 100Mo nuclei using molybdate crystal scintillators, operating at milli-Kelvin (mK) temperatures, with a total of 80 kg of 100Mo. The background goal for the experiment is 10–4 counts/keV/kg/year in the region of interest around the 0νββ decay Q-value of 3,034 keV. To achieve this level, the rate of background signals arising from emissions produced by decays of radioactive impurities in the detector and shielding materials must be strictly controlled. To do this, concentrations of such impurities are measured and are controlled through materials selection and purification. In this paper, we describe the design and the construction materials used to build the AMoRE-II detector and shielding system, including active and passive shielding, the cryostat, and the detector holders and instrumentation, and we report on measurements of radioactive impurities within candidate and selected materials.

Abstract Breeding bulls are well suited to investigate inherited variation in male fertility because they are genotyped and their reproductive success is monitored through semen analyses and thousands of artificial inseminations. However, functional data from relevant tissues are lacking in cattle, which prevents fine-mapping fertility-associated genomic regions. Here, we characterize gene expression and splicing variation in testis, epididymis, and vas deferens transcriptomes of 118 mature bulls and conduct association tests between 414,667 molecular phenotypes and 21,501,032 genome-wide variants to identify 41,156 regulatory loci. We show broad consensus in tissue-specific and tissue-enriched gene expression between the three bovine tissues and their human and murine counterparts. Expression- and splicing-mediating variants are more than three times as frequent in testis than epididymis and vas deferens, highlighting the transcriptional complexity of testis. Finally, we identify genes (WDR19, SPATA16, KCTD19, ZDHHC1) and molecular phenotypes that are associated with quantitative variation in male fertility through transcriptome-wide association and colocalization analyses.

Advancements in sequencing and screening technology have made monoclonal antibodies more accessible, cost-effective, and precise. These drugs effectively target pathogens and cancer cells and even regulate metabolic pathways by focusing on specific intermediates. Monoclonal antibodies play a key role in mitigating a rise in occupation-related cancers, neurodegenerative disorders, and multidrug-resistant organisms. Here, we review the origins, mechanisms, and applications of this important drug class and explore future avenues for research.

Abstract Background Structural variations (SVs) in individual genomes are major determinants of complex traits, including adaptability to environmental variables. The Mongolian and Hainan cattle breeds in East Asia are of taurine and indicine origins that have evolved to adapt to cold and hot environments, respectively. However, few studies have investigated SVs in East Asian cattle genomes and their roles in environmental adaptation, and little is known about adaptively introgressed SVs in East Asian cattle. Results In this study, we examine the roles of SVs in the climate adaptation of these two cattle lineages by generating highly contiguous chromosome-scale genome assemblies. Comparison of the two assemblies along with 18 Mongolian and Hainan cattle genomes obtained by long-read sequencing data provides a catalog of 123,898 nonredundant SVs. Several SVs detected from long reads are in exons of genes associated with epidermal differentiation, skin barrier, and bovine tuberculosis resistance. Functional investigations show that a 108-bp exonic insertion in SPN may affect the uptake of Mycobacterium tuberculosis by macrophages, which might contribute to the low susceptibility of Hainan cattle to bovine tuberculosis. Genotyping of 373 whole genomes from 39 breeds identifies 2610 SVs that are differentiated along a “north–south” gradient in China and overlap with 862 related genes that are enriched in pathways related to environmental adaptation. We identify 1457 Chinese indicine-stratified SVs that possibly originate from banteng and are frequent in Chinese indicine cattle. Conclusions Our findings highlight the unique contribution of SVs in East Asian cattle to environmental adaptation and disease resistance.

Abstract Neonicotinoid pesticides are well-known for their sublethal effects on insect behavior and physiology. Recent work suggests neonicotinoids can impair insect olfactory processing, with potential downstream effects on behavior and possibly survival. However, it is unclear whether impairment occurs during peripheral olfactory detection, during information processing in central brain regions, or in both contexts. We used Drosophila melanogaster to explore the potential for neonicotinoids to disrupt olfaction by conducting electrophysiological analyses of single neurons and whole antennae of flies exposed to varying concentrations of the neonicotinoid imidacloprid (IMD) that were shown to cause relative differences in fly survival. Our results demonstrated that IMD exposure significantly reduced the activity of a single focal olfactory neuron and delayed the return to baseline activity of the whole antenna. To determine if IMD also impacts olfactory-guided behavior, we compared flies’ relative preference for odor sources varying in ethanol content. Flies exposed to IMD had a greater relative preference for ethanol-laced pineapple juice than control flies, demonstrating that neuronal shifts induced by IMD that we observed are associated with changes in relative preference. Given the interest in the sensory impacts of agrochemical exposure on wild insect behavior and physiology, we highlight the potential of Drosophila as a tractable model for investigating the effects of pesticides at scales ranging from single-neuron physiology to olfactory-guided behavior.

Abstract Background Low-pass sequencing followed by sequence variant genotype imputation is an alternative to the routine microarray-based genotyping in cattle. However, the impact of haplotype reference panels and their interplay with the coverage of low-pass whole-genome sequencing data have not been sufficiently explored in typical livestock settings where only a small number of reference samples is available. Methods Sequence variant genotyping accuracy was compared between two variant callers, GATK and DeepVariant, in 50 Brown Swiss cattle with sequencing coverages ranging from 4- to 63-fold. Haplotype reference panels of varying sizes and composition were built with DeepVariant based on 501 individuals from nine breeds. High-coverage sequence data for 24 Brown Swiss cattle were downsampled to between 0.01- and 4-fold to mimic low-pass sequencing. GLIMPSE was used to infer sequence variant genotypes from the low-pass sequencing data using different haplotype reference panels. The accuracy of the sequence variant genotypes that were inferred from low-pass sequencing data was compared with sequence variant genotypes called from high-coverage data. Results DeepVariant was used to establish bovine haplotype reference panels because it outperformed GATK in all evaluations. Within-breed haplotype reference panels were more accurate and efficient to impute sequence variant genotypes from low-pass sequencing than equally-sized multibreed haplotype reference panels for all target sample coverages and allele frequencies. F1 scores greater than 0.9, which indicate high harmonic means of recall and precision of called genotypes, were achieved with 0.25-fold sequencing coverage when large breed-specific haplotype reference panels (n = 150) were used. In absence of such large within-breed haplotype panels, variant genotyping accuracy from low-pass sequencing could be increased either by adding non-related samples to the haplotype reference panel or by increasing the coverage of the low-pass sequencing data. Sequence variant genotyping from low-pass sequencing was substantially less accurate when the reference panel lacked individuals from the target breed. Conclusions Variant genotyping is more accurate with DeepVariant than GATK. DeepVariant is therefore suitable to establish bovine haplotype reference panels. Medium-sized breed-specific haplotype reference panels and large multibreed haplotype reference panels enable accurate imputation of low-pass sequencing data in a typical cattle breed.

Abstract Background Several models and algorithms have been proposed to build pangenomes from multiple input assemblies, but their impact on variant representation, and consequently downstream analyses, is largely unknown. Results We create multi-species super-pangenomes using pggb, cactus, and minigraph with the Bos taurus taurus reference sequence and eleven haplotype-resolved assemblies from taurine and indicine cattle, bison, yak, and gaur. We recover 221 k nonredundant structural variations (SVs) from the pangenomes, of which 135 k (61%) are common to all three. SVs derived from assembly-based calling show high agreement with the consensus calls from the pangenomes (96%), but validate only a small proportion of variations private to each graph. Pggb and cactus, which also incorporate base-level variation, have approximately 95% exact matches with assembly-derived small variant calls, which significantly improves the edit rate when realigning assemblies compared to minigraph. We use the three pangenomes to investigate 9566 variable number tandem repeats (VNTRs), finding 63% have identical predicted repeat counts in the three graphs, while minigraph can over or underestimate the count given its approximate coordinate system. We examine a highly variable VNTR locus and show that repeat unit copy number impacts the expression of proximal genes and non-coding RNA. Conclusions Our findings indicate good consensus between the three pangenome methods but also show their individual strengths and weaknesses that need to be considered when analysing different types of variants from multiple input assemblies.

Volcanogenic tsunami and wave hazard remains less understood than that of other tsunami sources. Volcanoes can generate waves in a multitude of ways, including subaqueous explosions. Recent events, including a highly explosive eruption at Hunga Tonga-Hunga Ha'apai and subsequent tsunami in January 2022, have reinforced the necessity to explore and quantify volcanic tsunami sources. We utilise a non-hydrostatic multilayer numerical method to simulate 20 scenarios of sublacustrine explosive eruptions under Lake Taupō, New Zealand, across five locations and four eruption sizes. Waves propagate around the entire lake within 15 min, and there is a minimum explosive size required to generate significant waves (positive amplitudes incident on foreshore of > 1 m) from the impulsive displacement of water from the eruption itself. This minimum size corresponds to a mass eruption rate of 5.8×107 kg s−1, or VEI 5 equivalent. Inundation is mapped across five built areas and becomes significant near shore when considering only the two largest sizes, above VEI 5, which preferentially impact areas of low-gradient slope. In addition, novel hydrographic output is produced showing the impact of incident waves on the Waikato River inlet draining the lake and is potentially useful for future structural impact analysis. Waves generated from these explosive source types are highly dispersive, resulting in hazard rapidly diminishing with distance from the source. With improved computational efficiency, a probabilistic study could be formulated and other, potentially more significant, volcanic source mechanisms should be investigated.

Abstract Liquid xenon time projection chambers are promising detectors to search for neutrinoless double beta decay (0 $$\nu \beta \beta $$ ν β β ), due to their response uniformity, monolithic sensitive volume, scalability to large target masses, and suitability for extremely low background operations. The nEXO collaboration has designed a tonne-scale time projection chamber that aims to search for 0 $$\nu \beta \beta $$ ν β β of $$^{136}$$ 136 Xe with projected half-life sensitivity of $$1.35\times 10^{28}$$ 1.35 × 10 28 yr. To reach this sensitivity, the design goal for nEXO is $$\le $$ ≤ 1% energy resolution at the decay Q-value ( $$2458.07\pm 0.31$$ 2458.07 ± 0.31 keV). Reaching this resolution requires the efficient collection of both the ionization and scintillation produced in the detector. The nEXO design employs Silicon Photo-Multipliers (SiPMs) to detect the vacuum ultra-violet, 175 nm scintillation light of liquid xenon. This paper reports on the characterization of the newest vacuum ultra-violet sensitive Fondazione Bruno Kessler VUVHD3 SiPMs specifically designed for nEXO, as well as new measurements on new test samples of previously characterised Hamamatsu VUV4 Multi Pixel Photon Counters (MPPCs). Various SiPM and MPPC parameters, such as dark noise, gain, direct crosstalk, correlated avalanches and photon detection efficiency were measured as a function of the applied over voltage and wavelength at liquid xenon temperature (163 K). The results from this study are used to provide updated estimates of the achievable energy resolution at the decay Q-value for the nEXO design.

Abstract The Advanced Mo-based Rare process Experiment (AMoRE)-Pilot experiment is an initial phase of the AMoRE search for neutrinoless double beta decay of $$^{100}$$ 100 Mo, with the purpose of investigating the level and sources of backgrounds. Searches for neutrinoless double beta decay generally require ultimately low backgrounds. Surface $$\alpha $$ α decays on the crystals themselves or nearby materials can deposit a continuum of energies that can be as high as the Q-value of the decay itself and may fall in the region of interest (ROI). To understand these background events, we studied backgrounds from radioactive contaminations internal to and on the surface of the crystals or nearby materials with Geant4-based Monte Carlo simulations. In this study, we report on the measured $$\alpha $$ α energy spectra fitted with the corresponding simulated spectra for six crystal detectors, where sources of background contributions could be identified through high energy $$\alpha $$ α peaks and continuum parts in the energy spectrum for both internal and surface contaminations. We determine the low-energy contributions from internal and surface $$\alpha $$ α contaminations by extrapolating from the $$\alpha $$ α background fitting model.

Pangenomes have a number of advantages over linear reference assemblies. Here the authors use bovine haplotype-resolved assemblies to show that structural variant-based pangenomes are consistent regardless of sequence platform, assembler, or coverage, suggesting that rigid protocols may not be required.

Abstract Background Stand and Move at Work was a 12-month, multicomponent, peer-led (intervention delivery personnel) worksite intervention to reduce sedentary time. Although successful, the magnitude of reduced sedentary time varied by intervention worksite. The purpose of this study was to use a qualitative comparative analysis approach to examine potential explanatory factors that could distinguish higher from lower performing worksites based on reduced sedentary time. Methods We assessed 12-month changes in employee sedentary time objectively using accelerometers at 12 worksites. We ranked worksites based on the magnitude of change in sedentary time and categorized sites as higher vs. lower performing. Guided by the integrated-Promoting Action on Research Implementation in Health Services framework, we created an indicator of intervention fidelity related to adherence to the protocol and competence of intervention delivery personnel (i.e., implementer). We then gathered information from employee interviews and surveys as well as delivery personnel surveys. These data were aggregated, entered into a truth table (i.e., a table containing implementation construct presence or absence), and used to examine differences between higher and lower performing worksites. Results There were substantive differences in the magnitude of change in sedentary time between higher (-75.2 min/8 h workday, CI95: -93.7, -56.7) and lower (-30.3 min/8 h workday, CI95: -38.3, -22.7) performing worksites. Conditions that were present in all higher performing sites included implementation of indoor/outdoor walking route accessibility, completion of delivery personnel surveys, and worksite culture supporting breaks (i.e., adherence to protocol). A similar pattern was found for implementer willingness to continue role and employees using face-to-face interaction/stair strategies (i.e., delivery personnel competence). However, each of these factors were also present in some of the lower performing sites suggesting we were unable to identify sufficient conditions to predict program success. Conclusions Higher intervention adherence and implementer competence is necessary for greater program success. These findings illustrate the need for future research to identify what factors may influence intervention fidelity, and in turn, effectiveness. Trial registration ClinicalTrials.gov Identifier: NCT02566317 . Registered 2 October 2015, first participant enrolled 11 January 2016.

Many youth experiencing homelessness (YEH) are surprisingly hopeful. We propose a typology of the hopes for the future (HFTF) of YEH, based on a qualitative study employing grounded theory. YEH fell into four categories: (a) nonmainstream HFTF youth who wished to escape the mainstream, (b) mainstream HFTF youth who wished to integrate into the mainstream, (c) marijuana-economy HFTF youth who aspired to escape homelessness by entering the marijuana economy, and (d) no HFTF youth who did not or could not envision the future. Our finding that some YEH transition between different HFTF suggests a modifiable point of intervention. We share novel findings regarding how the perceived trajectories of some YEH may have been influenced by the evolving legal status of marijuana. Our emic approach to constructing a typology may inform the design of more acceptable/effective interventions. We suggest implications, including ethical quandaries, raised by our findings.

ObjectiveTo examine the association between self-reported racial discrimination and allostatic load, and whether the association differs by socioeconomic position.MethodsWe recruited a purposive cross-section of midlife (ages 30-50) African American women residing in four San Francisco Bay area counties (n = 208). Racial discrimination was measured using the Experience of Discrimination scale. Allostatic load was measured as a composite of 15 biomarkers assessing cardiometabolic, neuroendocrine, and inflammatory activity. We calculated four composite measures of allostatic load and three system-specific measures of biological dysregulation. Multivariable regression was used to examine associations, while adjusting for relevant confounders.ResultsIn the high education group, reporting low (b = -1.09, P = .02, 95% CI = -1.99, -0.18) and very high (b = -1.88, P = .003, 95% CI = -3.11, -0.65) discrimination was associated with lower allostatic load (reference=moderate). Among those with lower education, reporting low (b = 2.05, P = .008, 95% CI = 0.55,3.56) discrimination was associated with higher allostatic load. Similar but less consistent associations were found for poverty status. Associations were similar for cardiometabolic functioning, but not for neuroendocrine or inflammatory activity.ConclusionsRacial discrimination may be an important predictor of cumulative physiologic dysregulation. Factors associated with educational attainment may mitigate this association for African American women and other groups experiencing chronic social stress.

This paper describes preparing radiopure molybdenum trioxide powder enriched with Mo-100 isotope for the AMoRE-II experiment. AMoRE-II, the second phase of the AMoRE experiments, will search for the neutrinoless double-beta decay (0νDBD) of the 100Mo isotope using over 100 kg of 100Mo embedded in 200 kg of ultra-pure Li2100MoO4 bolometric crystals. Efficient purification technology was developed and adapted to purify 100MoO3 powder with a 5 kg per month production capacity. Based on the ICP-MS analysis of purified powder, the 232Th and 238U were reduced to

Abstract We present a background model for dark matter searches using an array of NaI(Tl) crystals in the COSINE-100 experiment that is located in the Yangyang underground laboratory. The model includes background contributions from both internal and external sources, including cosmogenic radionuclides and surface $$^{210}$$ 210 Pb contamination. To build the model in the low energy region, with a threshold of 1 keV, we used a depth profile of $$^{210}$$ 210 Pb contamination in the surface of the NaI(Tl) crystals determined in a comparison between measured and simulated spectra. We also considered the effect of the energy scale errors propagated from the statistical uncertainties and the nonlinear detector response at low energies. The 1.7 years COSINE-100 data taken between October 21, 2016 and July 18, 2018 were used for this analysis. Our Monte Carlo simulation provides a non-Gaussian peak around 50 keV originating from beta decays of bulk $$^{210}$$ 210 Pb in a good agreement with the measured background. This model estimates that the activities of bulk $$^{210}$$ 210 Pb and $$^{3}$$ 3 H are dominating the background rate that amounts to an average level of $$2.85\pm 0.15$$ 2.85 ± 0.15 counts/day/keV/kg in the energy region of (1–6) keV, using COSINE-100 data with a total exposure of 97.7 kg $$\cdot $$ · years.

Abstract We report the identification of metastable isomeric states of $$^{228}$$ 228 Ac at 6.28 keV, 6.67 keV and 20.19 keV, with lifetimes of an order of 100 ns. These states are produced by the $$\beta $$ β -decay of $$^{228}$$ 228 Ra, a component of the $$^{232}$$ 232 Th decay chain, with $$\beta $$ β Q-values of 39.52 keV, 39.13 keV and 25.61 keV, respectively. Due to the low Q-value of $$^{228}$$ 228 Ra as well as the relative abundance of $$^{232}$$ 232 Th and their progeny in low background experiments, these observations potentially impact the low-energy background modeling of dark matter search experiments.

Control efforts to reduce infection from the parasitic flatworm Opisthorchis viverrini have progressed through understanding the epidemiology of Opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. Yet the problem persists. The case study method was used to examine the fundamental cause of the liver fluke infection problem. Evidence shows that the liver fluke–infected population does not care about living a long life. For them, suffering and death are simply a part of life, and expected. Thus, the cause(s) leading to death is not important. They believe morally bad actions, and predetermined fate associated with kamma in Buddhism, play a big role whether or not one is infected with the liver fluke. Health interventions may be made more effective if they take into account the liver fluke–infected population’s worldviews about ethics, morality, life, and death. We researchers should not feel concerned only about medically determined causes of death.

Summary: Nectar chemistry can influence the behavior of pollinators in ways that affect pollen transfer, yet basic questions about how nectar chemical diversity impacts plant-pollinator relationships remain unexplored. For example, plants’ capacity to produce neurotransmitters and endocrine disruptors may offer a means to manipulate pollinator behavior. We surveyed 15 plant species and discovered that two insect neurotransmitters, octopamine and tyramine, were widely distributed in floral nectar. We detected the highest concentration of these chemicals in Citrus, alongside the well-studied alkaloid caffeine. We explored the separate and interactive effects of these chemicals on insect pollinators in a series of behavioral experiments on bumblebees (Bombus impatiens). We found that octopamine and tyramine interacted with caffeine to alter key aspects of bee behavior relevant to plant fitness (sucrose responsiveness, long-term memory, and floral preferences). These results provide evidence for a means by which synergistic or antagonistic nectar chemistry might influence pollinators.

This paper shows that railroad building in Russia, as in Europe and the US in the nineteenth century, improved the value of land, a classic benefit of transportation investment in largely agrarian countries. From a database constructed for this paper, we use cross-sectional data for the fifty European Russian regions to show the association of the length of the railroad (measured in 1894), land prices (measured in 1900) and annual growth of land prices (in rubles) for 1885–1910.

We are living in a time of rapid biodiversity loss. Numerous studies have shown that modern extinction rates are higher than pre-human background rates. However, these studies of biodiversity decline almost exclusively focus on large vertebrates. The scientific community lacks the sufficient long-term records necessary to track biodiversity loss for many invertebrate taxa. However, aquatic, benthic, and skeletonized invertebrates have the advantage of leaving a long-term record that can readily be sampled in conjunction with living communities because the mineralized skeletons accumulate in the very same sediments in which the animals that produced them once lived. These not-quite-fossil “death assemblages” contain an underutilized record for long-term monitoring. Here, we leverage three case studies of calcareous micro- and macro-faunal remains from three aquatic environments spanning two gradients: freshwater to fully marine and polluted to pristine and remediated. We compared the death assemblages to living assemblages in these case studies using Spearman’s rho and the Jaccard–Chao agreement to determine the degree of fidelity. Death assemblages of lacustrine, calcareous microcrustaceans (Ostracoda), collected from lakes in The Bahamas and Wisconsin, USA, faithfully record human impacts, both for degradation and remediation, as determined by a mismatch in the live–dead comparisons. Likewise, the live–dead comparisons of calcareous marine macrofauna (Bivalvia) from the southern California shelf also indicate human impact, including pollution and remediation. These case studies demonstrate how death assemblages can be used to gauge the changes in community assembly and population structures at local and regional scales, even in the absence of a systemic monitoring program. Conservation, restoration, and biomonitoring efforts would benefit from the inclusion of live–dead comparisons of taxa with easily fossilized, identifiable parts. Live–dead studies, such as those presented in these case studies, can be used as tools for recognizing targets and establishing baselines for conservation, tracking community responses to remediation efforts, and identifying local species extinctions.

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An understanding of factors influencing health in socioeconomic groups is required to reduce health inequalities. This study investigated combinations of health determinants associated with self-rated health (SRH), and their relative importance, in income-based groups. Cross-sectional data from year 15 (2000 - 2001) of the CARDIA study (Coronary Artery Risk Development in Young Adults, USA) - 3648 men and women (mean 40 years) - were split into 5 income-based groups. SRH responses were categorized as 'higher'/'lower'. Health determinants (medical, lifestyle, and social factors, living conditions) associated with SRH in each group were analyzed using classification tree analysis (CTA). Income and SRH were positively associated (p

Abstract LUX-ZEPLIN (LZ) is a second-generation direct dark matter experiment with spin-independent WIMP-nucleon scattering sensitivity above $${1.4 \times 10^{-48}}\, {\hbox {cm}}^{2}$$ 1.4 × 10 - 48 cm 2 for a WIMP mass of $${40}\, \hbox {GeV}/{\hbox {c}}^{2}$$ 40 GeV / c 2 and a $${1000}\, \hbox {days}$$ 1000 days exposure. LZ achieves this sensitivity through a combination of a large $${5.6}\, \hbox {t}$$ 5.6 t fiducial volume, active inner and outer veto systems, and radio-pure construction using materials with inherently low radioactivity content. The LZ collaboration performed an extensive radioassay campaign over a period of six years to inform material selection for construction and provide an input to the experimental background model against which any possible signal excess may be evaluated. The campaign and its results are described in this paper. We present assays of dust and radon daughters depositing on the surface of components as well as cleanliness controls necessary to maintain background expectations through detector construction and assembly. Finally, examples from the campaign to highlight fixed contaminant radioassays for the LZ photomultiplier tubes, quality control and quality assurance procedures through fabrication, radon emanation measurements of major sub-systems, and bespoke detector systems to assay scintillator are presented.

Pregnant women are at a high risk for experiencing sleep disturbances, excess energy intake, low physical activity, and excessive gestational weight gain (GWG). Scant research has examined how sleep behaviors influence energy intake, physical activity, and GWG over the course of pregnancy. This study conducted secondary analyses from the Healthy Mom Zone Study to examine between- and within-person effects of weekly sleep behaviors on energy intake, physical activity, and GWG in pregnant women with overweight/obesity (PW-OW/OB) participating in an adaptive intervention to manage GWG. The overall sample of N = 24 (M age = 30.6 years, SD = 3.2) had an average nighttime sleep duration of 7.2 h/night. In the total sample, there was a significant between-person effect of nighttime awakenings on physical activity; women with >1 weekly nighttime awakening expended 167.56 less physical activity kcals than women with

Volcanic ballistics are the main hazard to life and infrastructure from Strombolian eruptions, which are a tourist drawcard, exposing people to this hazard. Most research to date has been to understand this style of eruption and how ballistics form and travel. However, little focus has been placed on how ballistics are distributed within ballistic fields or the inclusion of this data into hazard and risk assessments. In this study we used a UAV to image the ballistic field, and cameras to record eruptions at Yasur Volcano, Vanuatu from 28 July – 2 August and 17 – 19 October 2016. We present the mapped distributions from the two trips, how the field changes with distance and direction from the vent, and how eruption dynamics influence these changes. Our evidence for directionality results in considerable variation in summit ballistic hazard and is an important consideration for ballistic hazard and risk assessments.

Silicic caldera volcanoes are frequently situated in regions of tectonic extension, such as continental rifts, and are subject to periods of unrest and/or eruption that can be triggered by the interplay between magmatic and tectonic processes. Modern (instrumental) observations of deformation patterns associated with magmatic and tectonic unrest in the lead up to eruptive events at silicic calderas are sparse. Therefore, our understanding of the magmatic-tectonic processes associated with volcanic unrest at silicic calderas is largely dependent on historical and geological observations. Here we utilize existing instrumental, historical and geological data to provide an overview of the magmatic-tectonic deformation patterns operating over annual to 104 year timescales at Taupō volcano, now largely submerged beneath Lake Taupō, in the rifted-arc of the Taupō Volcanic Zone. Short-term deformation patterns observed from seismicity, lake level recordings and historical records are characterized by decadal-scale uplift and subsidence with accompanying seismic swarms, ground shaking and surface ruptures, many of which may reflect magma injections into and around the magma reservoir. The decadal-scale frequency at which intense seismic events occur shows that ground shaking, rather than volcanic eruptions, is the primary short-term local hazard in the Taupō District. Deformation trends near and in the caldera on 101–104 yr timescales are atypical of the longer-term behavior of a continental rift, with magma influx within the crust suppressing axial subsidence of the rift basin within ∼10 km of the caldera margin. Examination of exposed faults and fissures reveals that silicic volcanic eruptions from Taupō volcano are characterized by intense syn-eruptive deformation that can occasionally extend up to 50 km outside the caldera structure, including ground shaking, fissuring and triggered fault movements. We conclude that eruption and unrest scenarios at Taupō volcano depend on the three-way coupling between the mafic-silicic-tectonic systems, with eruption and/or unrest events leading to six possible outcomes initially triggered by mafic injection either into or outside the magma mush system, or by changes to the tectonic stress state.

Utilization of frontal balloon sinuplasty in pediatric complicated acute rhinosinusitis (ARS) is demonstrated to be a safe and expedient alternative to other procedures such as trephination or functional endoscopic sinus surgery (FESS) in this case series. We performed a retrospective review of six pediatric cases of frontal balloon sinuplasty for ARS with intracranial complications at a tertiary academic center. Patients underwent unilateral (n = 5) or bilateral dilation (n = 1) in addition to functional endoscopic sinus surgery (FESS) including anterior ethmoidectomy (n = 5) and maxillary antrostomy (n = 6). This technique effectively addressed frontal sinus obstruction and served as an alternative to procedures such as trephination or functional endoscopic sinus surgery. No immediate or short-term complications of balloon dilation were observed in these cases. A larger cohort and extended follow-up are necessary to determine the use and long-term impact of this technique.

Social class gradients have been explored in adults and children, but not extensively during adolescence. The first objective of this study was to examine the association between adolescent risk behaviors and a new indicator of adolescent relative social position, adolescent “perceived social mobility.” Second, it investigated potential underlying demographic, socioeconomic, and psychosocial determinants of this indicator. Data were taken from the 2004 urban adolescent module of Oportunidades, a cross-sectional study of Mexican adolescents living in poverty. Perceived social mobility was calculated for each subject by taking the difference between their rankings on two 10-rung ladder scales that measured (1) projected future social status and (2) current subjective social status within Mexican society. Adolescents with higher perceived social mobility were significantly less likely to report alcohol consumption, drinking with repercussions, compensated sex, police detainment, physical fighting, consumption of junk food or soda, or watching ≥4 h of television during the last viewing. They were significantly more likely to report exercising during the past week and using a condom during last sexual intercourse. These associations remained significant with the inclusion of covariates, including parental education and household expenditures. Multiple logistic regression analyses show higher perceived social mobility to be associated with staying in school longer and having higher perceived control. The present study provides evidence for the usefulness of perceived social mobility as an indicator for understanding the social gradient in health during adolescence. This research suggests the possibility of implementing policies and interventions that provide adolescents with real reasons to be hopeful about their trajectories.

Abstract Reliable methods for volcanic impact and risk assessments are essential. They provide constructive information to emergency and disaster managers, critical infrastructure providers, the insurance industry, and wider society. Post-eruption clean-up of tephra deposits is a prevalent and expensive (time and resource) activity which is often not planned for. Here, we present an overview of the clean-up efforts undertaken in four communities after the VEI 4 eruption of Calbuco volcano in 2015. We narratively reconstruct clean-up efforts in Ensenada (Chile), Junín de los Andes (Argentina), San Martín de los Andes (Argentina), and Villa La Angostura (Argentina) using semi-structured interviews, syn- and post-deposition photographs, pre- and post-event visual spectrum satellite imagery, and media reports. We compare these reconstructions with estimates based on a geospatial modelling approach adapted from Hayes et al. (Journal of Applied Volcanology 6:1; 2017). Specifically, we compare reported and geospatially derived estimates for volume of tephra removed, and clean-up operation duration. Our modelling approach performed well for Junín de los Andes but did not adequately capture volume and clean-up operation duration for the three remaining case study locations. We discuss several sources of uncertainty (including observational errors and natural variance of tephra deposit thickness), reported tephra removal volume estimates, clean-up methods, land use, and temporal evolution of clean-up operation demand. Our work demonstrates the utility of using simple geospatial data to develop assessments for tephra clean-up for use in response and recovery planning, and quantitative volcanic impact and risk assessments.

Abstract Limits on the cross section for weakly interacting massive particles (WIMPs) elastic scattering on nuclei in NaI(Tl) detectors at the Yangyang Underground Laboratory are obtained from a 2967.4 kg·day data exposure. The nuclei recoiling from the scattering process are identified by the pulse shape of the scintillation light signals that they produce. The data are consistent with a no nuclear-recoil hypothesis, and WIMP-mass-dependent 90% confidence-level upper-limits are set on WIMP-nuclei elastic scattering cross sections. These limits partially exclude the DAMA/LIBRA allowed region for WIMP-sodium interactions with the same NaI(Tl) target material. The 90% confidence level upper limit on the WIMP-nucleon spin-independent cross section is 3.26×10−4 pb for a WIMP mass of 10 GeV/c2.

We study the effects of non-determinism and gene duplication on the structure of genotype–phenotype (GP) maps by introducing a non-deterministic version of the Polyomino self-assembly model. This model has previously been used in a variety of contexts to model the assembly and evolution of protein quaternary structure. Firstly, we show the limit of the current deterministic paradigm which leads to built-in anti-correlation between evolvability and robustness at the genotypic level. We develop a set of metrics to measure structural properties of GP maps in a non-deterministic setting and use them to evaluate the effects of gene duplication and subsequent diversification. Our generalized versions of evolvability and robustness exhibit positive correlation for a subset of genotypes. This positive correlation is only possible because non-deterministic phenotypes can contribute to both robustness and evolvability. Secondly, we show that duplication increases robustness and reduces evolvability initially, but that the subsequent diversification that duplication enables has a stronger, inverse effect, greatly increasing evolvability and reducing robustness relative to their original values.