76 results on '"Ruijuan Hao"'
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2. Identification and characterization of circRNAs from different body color leopard coral grouper (Plectropomus leopardus)
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Ruijuan Hao, Xiaowen Zhu, Changxu Tian, Yang Huang, Guangli Li, and Chunhua Zhu
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Plectropomus leopardus ,circRNA ,ceRNA ,body color ,pigmentation ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Circular RNAs (circRNAs) play key roles in several biological processes in animals and their regulatory mechanism in body color formation or pigmentation in fish remains unclear. Here, circRNAs from black and red individuals of Plectropomus leopardus were identified to clarify the mechanism of circRNAs and the competing endogenous RNA (ceRNA) network (circRNA-microRNA (miRNA)-messenger RNA (mRNA)) in body color formation. We detected a total of 1,424 novel circRNAs. Expression analysis of circRNAs in black vs. red P. leopardus revealed 24 differentially expressed circRNAs (DECs), and 11 and 13 of these DECs were up-regulated and down-regulated in red individuals relative to black individuals, respectively (P1). We identified a total of 19 significant miRNA-circRNA-mRNA ceRNA networks through the analysis of DECs, differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs). Pathway enrichment analyses of the DEGs involved in the ceRNA network revealed that they were mainly involved in melanin metabolism and immune response. Our findings showed the possibility of the regulatory functions of circRNAs and the corresponding ceRNA network in the body color formation process and will aid the breeding selection process of P. leopardus.
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- 2023
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3. LncRNA–miRNA–mRNA ceRNA network of different body colors in Plectropomus leopardus
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Ruijuan Hao, Xiaowen Zhu, Changxu Tian, Mouyan Jiang, Yang Huang, Guangli Li, and Chunhua Zhu
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Plectropomus leopardus ,lncRNA ,ceRNA network ,body color ,miRNA ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Long non-coding RNAs (lncRNAs) play a multifaceted role in transcriptional regulation, and the potential molecular regulatory mechanisms of lncRNAs and lncRNA–miRNA–mRNA networks in body color formation are of great significance for its selective breeding. Therefore, lncRNAs and lncRNA-miRNA-mRNA ceRNA network of red- and black-colored Plectropomus leopardus were identified and analyzed. Sequencing analyses identified 167 differentially expressed lncRNAs (DELs) between red- and black-colored P. leopardus, including 89 upregulated and 78 downregulated DELs in the red-colored group (false discovery rate (FDR) < 0.05 and |log2FC| > 1). Differentially expressed miRNA (DEM), genes (DEG), and DEL analyses found 605 and 125 negatively co-expressed miRNA–mRNA pairs and lncRNA–miRNA pairs, respectively. Further correlation analysis with Spearman’s correlation coefficient >0.9 as the threshold identified 3,721 lncRNA–mRNA pairs. Then, a competitive endogenous RNA (ceRNA) network of 325 pairs (p < 0.05) was obtained. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of network DEGs showed that melanin metabolic process, lipid metabolism, and immune-related pathway were enriched. The ceRNA network provided interactions among lncRNAs, miRNAs, and mRNAs and extended the molecular foundation of body color formation.
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- 2023
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4. RNA-seq of hypo- and hyper-salinity stress-response transcriptome in the liver of greater amberjack (Seriola dumerili) juveniles
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Xiaoying Ru, Yang Huang, Hongjuan Shi, Yuhao Peng, Ruijuan Hao, Tonglin Yang, Kunfeng Zhu, Guangli Li, and Chunhua Zhu
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Seriola dumerili ,Salinity stress ,Liver ,Differentially expressed genes ,Transcriptome ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Salinity is an important abiotic stress that has significant effects on the physiology and metabolism of marine fish. The greater amberjack (Seriola dumerili) is a large, fast-growing species with high commercial value in global aquaculture. To describe the molecular response of the greater amberjack liver to different salinity stresses, RNA-seq analysis was performed to identify the important genes and signaling pathways activated in response to salt stress. Greater amberjack juveniles were reared under different salinity stresses (20, 30, and 40 ppt) for 30 days to evaluate their tolerance, adaptability, and molecular responses. A total of 657 (426 up-regulated and 231 down-regulated) and 65 (17 up-regulated and 48 down-regulated) differentially expressed genes (DEGs) were identified in the group at 30 vs. 20 ppt and 30 vs. 40 ppt salinity, respectively. qPCR and transcriptomic analysis showed that salinity stress affected the expression of genes involved in lipid metabolism (pld2, pla2g7, acacb, and acsl4a), vitamin metabolism (cyp24a1 and cyp2r1), ion transporters (slc4a1a, slc4a4b, rhag, and rh50), and signal transduction (itpkcb, fgf19, and fgfr4). KEGG pathway enrichment analysis showed that the DEGs were primarily involved in metabolism, ribosome biogenesis in eukaryotes, and insulin signaling pathway. The identified candidate genes involved in metabolism pathways, ion transporters, and signal transduction, provide a basis for further study of the molecular mechanisms involved in salinity adaptation and transcriptional plasticity in the livers of marine fish.
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- 2023
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5. Lipidomic insights into the immune response and pearl formation in transplanted pearl oyster Pinctada fucata martensii
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Hailing Wu, Chuangye Yang, Ruijuan Hao, Yongshan Liao, Qingheng Wang, and Yuewen Deng
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Pinctada fucata martensii ,lipidomics ,transplantation ,immune response ,pearl formation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
During pearl culture, the excess immune responses may induce nucleus rejection and death of pearl oysters after transplantation. To better understand the immune response and pearl formation, lipidomic analysis was applied to investigate changes in the serum lipid profile of pearl oyster Pinctada fucata martensii following transplantation. In total, 296 lipid species were identified by absolute quantitation. During wound healing, the content of TG and DG initially increased and then decreased after 3 days of transplantation with no significant differences, while the level of C22:6 decreased significantly on days 1 and 3. In the early stages of transplantation, sphingosine was upregulated, whereas PC and PUFAs were downregulated in transplanted pearl oyster. PI was upregulated during pearl sac development stages. GP and LC-PUFA levels were upregulated during pearl formation stage. In order to identify enriched metabolic pathways, pathway enrichment analysis was conducted. Five metabolic pathways were found significantly enriched, namely glycosylphosphatidylinositol-anchor biosynthesis, glycerophospholipid metabolism, alpha-linolenic acid metabolism, linoleic acid metabolism and arachidonic acid metabolism. Herein, results suggested that the lipids involved in immune response, pearl sac maturation, and pearl formation in the host pearl oyster after transplantation, which might lead to an improvement in the survival rate and pearl quality of transplanted pearl oyster.
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- 2022
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6. Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii
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Shaojie Zhu, Yubo He, Qiongyu Xu, Jiabin Zhang, Chuangye Yang, Ruijuan Hao, Junhui Li, and Yuewen Deng
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Pinctada fucata martensii ,Pm5-HTR2 ,Pm5-HTR4 ,larval metamorphosis ,molecular cloning ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii (Pm5-HTR2 and Pm5-HTR4) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2, and a 1185 bp open reading frame was detected in Pm5-HTR4; these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis.
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- 2022
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7. Analysis of body color formation of leopard coral grouper Plectropomus leopardus
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Ruijuan Hao, Xiaowen Zhu, Changxu Tian, Chunhua Zhu, and Guangli Li
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body color ,Plectropomus leopardus ,mRNA ,metabolite ,miRNA ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Body color is an essential ecological phenotypic trait determining the ability of aquatic animals to adapt to or survive in a treacherous environment. However, researches regarding its underlying molecular mechanism in leopard coral groupers has been limited. Therefore, mRNAs, metabolites, and miRNAs of different-colored leopard coral grouper (Plectropomus leopardus) were sequenced to investigate the body color formation mechanism. The transcriptome analysis identified 1236 genes as differentially expressed genes (DEGs), of which 579 were up-regulated and 657 were down-regulated in the red-colored group compared with the brown-colored group (FDR < 0.01 and |log2FC| > 1). Metabolome analysis revealed 439 and 84 SDMs in POS and NEG of liquid chromatography-mass spectrometry, respectively (VIP > 1 and P < 0.05). The miRNA analysis exhibited 149 (120 up-regulated and 29 down-regulated) differentially expressed miRNAs (DEMs) in the analysis of the brown-colored vs. red-colored groups (P < 0.05 and |log2FC| > 1), including miR-144, miR-185, miR-192, miR-2188, miR-6316, novel-m0015, and novel-m0040. Integrated analysis of multiple omics exhibited that carotenoid metabolism, immune response, lipid metabolism, and amino acid metabolism were involved in the body color formation and adaptation to the environment. Therefore, the present multiomics research prepared a foundation for bright body color formation and promoted the process of preferred body-color breeding.
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- 2022
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8. Improved growth performance, digestive ability, antioxidant capacity, immunity and Vibrio harveyi resistance in coral trout (Plectropomus leopardus) with dietary vitamin C
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Xiaowen Zhu, Ruijuan Hao, Junpeng Zhang, Changxu Tian, Yucong Hong, Chunhua Zhu, and Guangli Li
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Growth performance ,Immunity ,Oxidation resistance ,Vibrio harveyi ,Vitamin C ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
An 8-week growth trial was conducted to assess the effects of dietary vitamin C (VC) on the growth performance, digestive enzyme activity, antioxidant ability, immunity, and resistance to Vibrio harveyi infection of coral trout (Plectropomus leopardus, initial weight 17.42 ± 0.06 g). Four kinds of iso-nitrogenous and iso-lipidic experimental feeds were formulated with covering VC levels of 0 (C0), 50 (C1), 100 (C2), and 200 (C3) mg/kg, respectively. Supplementation of an appropriate amount of VC in diet (a) increased specific growth rate (SGR) and weight gain rate (WGR); (b) increased amylase, lipase, and trypsin activities in the liver; (c) enhanced glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and reduced malonic dialdehyde (MDA) level in serum and liver; (d) increased lysozyme (LZ) and acid phosphatase (ACP) activities in serum and liver, as well as complement (C3 and C4) and immunoglobulin M (IgM) contents in the liver; (e) upregulated the relative expression of copper-zinc superoxide dismutase (SOD-1), manganese superoxide dismutase (SOD-2), CAT, GSH-Px1a, ACP6, AKP, LZ-c, IgM, C3, and C4-b in the liver; (f) improved the survival rate of V. harveyi challenge. In summary, an appropriate amount of VC could improve growth, digestive ability, antioxidant capacity, immunity, and the ability to resist the challenge of V. harveyi in coral trout. In order to ensure that coral trout has good growth performance, digestive and antioxidant capacity, it is recommended that the levels of VC in the feed is between 255.25 and 273.84 mg/kg. The immunity of coral trout is stronger when the VC level in the diet is between 251.95 and 314.03 mg/kg.
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- 2022
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9. Sex-Inclined Piwi-Interacting RNAs in Serum Exosomes for Sex Determination in the Greater Amberjack (Seriola dumerili)
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Qiuxia Deng, Na Zhao, Xiaoying Ru, Ruijuan Hao, Bo Zhang, and Chunhua Zhu
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piwi-interacting RNAs ,serum exosome ,Seriola dumerili ,sex inclination ,molecular markers ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The greater amberjack (Seriola dumerili) is a gonochoristic fish with no sexual dimorphism in appearance, making sex identification difficult. Piwi-interacting RNAs (piRNAs) function in transposon silencing and gametogenesis and are involved in various physiological processes, including sex development and differentiation. Exosomal piRNAs can be indicators for the determination of sex and physiological status. In this study, four piRNAs were differentially expressed in both serum exosomes and gonads between male and female greater amberjack. Three piRNAs (piR-dre-32793, piR-dre-5797, and piR-dre-73318) were significantly up-regulated and piR-dre-332 was significantly down-regulated in serum exosomes and gonads of male fish, compared to female fish, consistent with the serum exosomal results. According to the relative expression of four marker piRNAs derived from the serum exosomes of greater amberjack, the highest relative expression of piR-dre-32793, piR-dre-5797, and piR-dre-73318 in seven female fish and that of piR-dre-332 in seven male fish can be used as the standard for sex determination. The method of sex identification can ascertain the sex of greater amberjack by blood collection from the living body, without sacrificing fish. The four piRNAs did not show sex-inclined expression in the hypothalamus, pituitary, heart, liver, intestine, and muscle tissue. A piRNA–target interaction network involving 32 piRNA-mRNA pairs was generated. Sex-related target genes were enriched in sex-related pathways, including oocyte meiosis, transforming growth factor-beta signaling pathway, progesterone-mediated oocyte maturation, and gonadotropin releasing hormone signaling pathway. These results provide a basis for sex determination in greater amberjack and improve our understanding of the mechanisms underlying sex development and differentiation in the species.
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- 2023
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10. Cloning and functional characterization of PmΔ5FAD in pearl oyster Pinctada fucata martensii
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Chuangye Yang, Ruijuan Hao, Chengzhang He, Yuewen Deng, and Qingheng Wang
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PmΔ5FAD ,PUFA biosynthesis ,Pearl oyster ,Pinctada fucata martensii ,Grafting operation ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Pinctada fucata martensii produces high-quality pearls. However, excess immune and inflammatory response after grafting operation will lead to nucleus rejection, pearl sac formation failure, and death of the host pearl oyster. Polyunsaturated fatty acids (PUFAs) are critically important for its regulation function in inflammation and their synthesis process was limited by the related enzymes including fatty acid desaturase (FAD). In this study, FAD gene in the pearl oyster was characterized in P. f. martensii (PmΔ5FAD). We established that the full-length sequence of PmΔ5FAD contained a 1302-bp open reading frame that encoded a sequence of 433 amino acids. PmΔ5FAD domain analysis revealed the presence of a distinctive Cyt-b5 domain and a FA-desaturase domain that were highly similar to the Δ5FAD protein sequences identified in Haliotis discus hannai (69.25%) and Mimachlamys nobilis (62.96%). PmΔ5FAD expressed in all detected tissues. Functional analysis of PmΔ5FAD in recombinant Saccharomyces cerevisiae yeast showed the existence of Δ5-desaturation activity, which towards PUFA substrates and it efficiently desaturated exogenous PUFA C20:3n-6 to C20:4n-6 (ARA) with a desaturation conversion rate of 19.49%. Furthermore, PmΔ5FAD showed a significantly higher expression level on day 1 after the grafting operation, which may upregulate ARA synthesis in response to inflammatory and immune. These results provide important information concerning the function of FADs during pearl oyster PUFA biosynthesis and grafting operation.
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- 2022
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11. Integrative Transcriptomics and Metabolomics Analysis of Body Color Formation in the Leopard Coral Grouper (Plectropomus leopardus)
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Xiaowen Zhu, Ruijuan Hao, Changxu Tian, Junpeng Zhang, Chunhua Zhu, and Guangli Li
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body color ,transcriptomics ,metabolomics ,pigment ,Plectropomus leopardus ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Body color is an important economic and ecological trait in aquatic animals, which influence their economic values and determine the animal ability to survive in the marine environment. Red-colored Plectropomus leopardus is in high demand owing to its bright color and potential value for artificial breeding. High-throughput transcriptome sequencing and liquid chromatography tandem-mass spectrometry (LC-MS/MS) approaches were used to identify transcript and metabolic differences between black-colored and red-colored P. leopardus. Compared with the black-colored group, 218 and 181 differentially expressed genes were up- and down- regulated, respectively, in the red-colored group. 425 and 56 significantly different metabolites were identified in LC-MS positive and LC-MS negative ion models, respectively, between two colored groups. Based on the integrative analysis, the red-colored group exhibited greater carotenoid uptake, transport, and accumulation activity potential than those in the black-colored group and may consume more arachidonic acid for body color formation. The black-colored group showed greater melanin synthesis activity compared with the red-colored group. These results substantially improve the understanding of molecular and metabolic mechanisms underlying body color formation in P. leopardus and the potential of body color for the environment adaptation.
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- 2021
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12. Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zhe Zheng, Ruijuan Hao, Xinwei Xiong, Yu Jiao, Yuewen Deng, and Xiaodong Du
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Shell formation ,Settlement ,Metamorphosis ,Molecular events ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Marine bivalves undergo complex development processes, such as shell morphology conversion and changes of anatomy and life habits. In this study, the transcriptomes of pearl oyster Pinctada fucata martensii and Pacific oyster Crassostrea gigas at different development stages were analyzed to determine the key molecular events related to shell formation, settlement and metamorphosis. Result According to the shell matrix proteome, biomineralization-related genes exhibited a consensus expression model with the critical stages of shell formation. Differential expression analysis of P. f. martensii, revealed the negative regulation and feedback of extracellular matrixs as well as growth factor pathways involved in shell formation of larvae, similar to that in C. gigas. Furthermore, neuroendocrine pathways in hormone receptors, neurotransmitters and neuropeptide receptors were involved in shell formation, settlement and metamorphosis. Conclusion Our research demonstrated the main clusters of regulation elements related to shell formation, settlement and metamorphosis. The regulation of shell formation and metamorphosis could be coupled forming the neuroendocrine-biomineralization crosstalk in metamorphosis. These findings could provide new insights into the regulation in bivalve development.
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- 2019
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13. Cloning and characterization of O-xylosyltransferase gene from Pinctada fucata martensii
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Ruijuan Hao, Zhe Zheng, Xiaodong Du, Yu Jiao, and Yuewen Deng
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pinctada fucata martensii ,xylosyltransferase oxt ,glycosaminoglycan ,proteoglycans ,biomineralization ,Veterinary medicine ,SF600-1100 - Abstract
Glycosaminoglycan (GAG), an important component of proteoglycan (PG), was biosynthesized with the initiation by peptide O-xylosyltransferase. O-xylosyltransferase activity was presented as a marker for increased PG synthesis. In the present study, a novel O-xylosyltransferase (OXT) gene was identified from Pinctada fucata martensii (PmOXT). The PmOXT-deduced protein sequence carried a typical water-soluble carbohydrate domain, branch domain, and xylosyltransferase domain. Homologous analysis of PmOXT presented the conserved DXD motif and catalytic structure characteristics. Phylogenetic tree analysis showed the traditional taxonomy and PmOXT clustered with Crassostrea gigas. PmOXT was expressed in all the detected tissues and developmental stages. PmOXT had a significantly higher expression level in the shell formation associated tissues and developmental stages. PmOXT expressed significantly decreased in the central zone of the mantle and marginal zone of the mantle after RNA interference; additionally, OXT activity and GAG content in the extrapallial fluid were significantly reduced compared with the control. Furthermore, the crystal tablets of prismatic layer displayed obvious holes and disordered crystals with obviously rough surface and irregular crystal tablets were observed in the nacre after RNAi. Results suggested that PmOXT affected the shell formation by influencing the formation of GAGs in the process of addition to the core proteins.
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- 2019
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14. Identification and Allelic Variants Associated With Cold Tolerance of PmPIAS in Pinctada fucata martensii
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Zhuoxin Lai, Linda Adzigbli, Qingyue Chen, Ruijuan Hao, Yongshan Liao, Yuewen Deng, and Qingheng Wang
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Pinctada fucata martensii ,PIAS ,cold tolerance ,SNPs ,expression pattern ,Physiology ,QP1-981 - Abstract
The protein inhibitor of activated STAT (PIAS) functions in diverse aspects, including immune response, cell apoptosis, cell differentiation, and proliferation. In the present study, the PIAS in the pearl oyster Pinctada fucata martensii was characterized. The sequence features of PmPIAS were similar to that of other PIAS sequences with PIAS typical domains, including SAP, Pro-Ile-Asn-Ile-Thr (PINIT), RLD domain, AD, and S/T-rich region. Homologous analysis showed that PmPIAS protein sequence showed the conserved primary structure compared with other species. Ribbon representation of PIAS protein sequences also showed a conserved structure among species, and the PINIT domain and RLD domain showed the conserved structure compared with the sequence of Homo sapiens. The expression pattern of PmPIAS in different tissues showed significant high expression in the gonad. PmPIAS also exhibited a significantly higher expression in the 1 and 2 days after cold tolerance stress (17°C) and showed its potential in the cold tolerance. The SNP analysis of the exon region of PmPIAS obtained 18 SNPs, and among them, 11 SNPs showed significance among different genotypes and alleles between cold tolerance selection line and base stock, which showed their potential in the breeding for cold tolerance traits.
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- 2021
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15. Transcriptomic analysis of differentially expressed genes in the larval settlement and metamorphosis of peanut worm Sipunculus nudus
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Fujun Cao, Ruzhuo Zhong, Chuangye Yang, Ruijuan Hao, Qingheng Wang, Yongshan Liao, and Yuewen Deng
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Sipunculus nudus ,transcriptomic ,settlement and metamorphosis ,larval ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Larval development, especially settlement and metamorphosis, has a vital influence on commercial culture during the life cycle of marine invertebrates. Sipunculus nudus, a peanut worm, is an important economic aquaculture species. However, information about the mechanism of its larval settlement and metamorphosis is still limited. Transcriptomic analysis was performed to determine the DEGs between the pelagospheric larva and creeping larva of S. nudus. A total of 96,455 unique unigenes were obtained and 46,355 were annotated. Transcriptome analysis revealed 19,352 DEGs, including 9,923 that were upregulated and 9,429 downregulated after settlement and metamorphosis. KEGG pathway enrichment analyses revealed that these DEGs were mainly involved in the Notch signaling pathway, TNF signaling pathway, protein digestion and absorption, thyroid hormone signaling pathway, and ECM–receptor interaction, and so on. Furthermore, the changes in genes related to cytoskeleton and cell adhesion indicated that protein degradation and apoptosis during metamorphosis mediate the loss of larval cilia and ECM remodeling functions in S. nudus metamorphosis. Changes in genes related to signal transduction and nervous system suggested the cessation of larval swimming, which is involved in settlement and metamorphosis. Changes in genes related to ingestion and digestion indicated that dietary shift occurred with metamorphic transition. Changes in some genes related to stress response and immunity indicated that conditioning induced the immune response of peanut worm. The results improve understanding of the physiological traits controlling S. nudus metamorphosis and provide a solid basis for further study.
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- 2020
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16. Characterization and development of SSR markers of Pinctada maxima by RNA-Seq approach
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Ziman Wang, Junhui Li, Ruijuan Hao, Linda Adzigbli, and Yuewen Deng
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Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
In this study, we developed microsatellite markers, analyzed their distribution and frequency and verified polymorphism based on the transcriptome data of pearl oyster Pinctada maxima. Totally, 58,121 potential EST-SSRs from 145,877 sequences were identified. We randomly selected 100 of these primer pairs for further validation in the stock, 76 primer pairs were successfully amplified and 25 primer pairs were found to be polymorphic. The number of alleles at each locus ranged from 2 to 5, with an average allele number of 2.96. The observed heterozygosity ranged from 0.185 to 0.931 with an average of 0.656. The expected heterozygosity ranged from 0.337 to 0.754 with an average of 0.552. The polymorphic information content ranged from 0.263 to 0.690 with an average of 0.462. These markers will provide an effective tool for genetic diversity, genetic map construction and molecular-assisted breeding in the species of P. maxima. Keywords: Pinctada maxima, SSR markers, Polymorphism, Genetic diversity
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- 2019
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17. Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii
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Chao Lei, Ruijuan Hao, Zhe Zheng, Yuewen Deng, Qingheng Wang, and Junhui Li
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Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 - Abstract
Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P
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- 2017
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18. Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii
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Qingheng Wang, Chuangye Yang, Ruijuan Hao, Zhe Zheng, Yu Jiao, Xiaodong Du, Yuewen Deng, and Ronglian Huang
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Biomineral ,Biomineralization ,Carbohydrate sulfotransferase 11 ,Chondroitin sulfate ,Chondroitin-4-sulfotransferase-1 (C4ST-1) ,Mantle tissue ,Molluscs ,Proteoglycans ,Real-time PCR ,RNA interference ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 - Abstract
Background: C4ST-1 catalyzes the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes (PmCHST11a and PmCHST11b) which encoded the C4ST-1 and explored the functions of these genes in the synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells. Results: Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a transmembrane domain. These properties indicated that these genes localize in the Golgi apparatus. Real-time PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in extrapallial fluid. Moreover, shell nacre crystallized irregularly with a rough surface after RNA interference. Conclusions: This study indicated that PmCHST11a and PmCHST11b are involved in the nacre formation of Pinctada fucata martensii through participating in the synthesis of chondroitin sulfate.
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- 2017
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19. Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii
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Ruijuan Hao, Chuchu Mo, Linda Adzigbli, Chuangye Yang, Yuewen Deng, and Qingheng Wang
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FGF18 ,SNP ,growth traits ,Pinctada fucata martensii ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii (PmFGF18) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A > C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits.
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- 2020
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20. Molecular cloning and characteristics analysis of Pmtgfbr1 from Pinctada fucata martensii
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Ruijuan Hao, Zhe Zheng, Xiaodong Du, Qingheng Wang, Junhui Li, Yuewen Deng, and Weiyao Chen
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Biotechnology ,TP248.13-248.65 - Abstract
Pinctada fucata martensii is cultured for pearl production. Growth improvement has received considerable research interest. Transforming growth factor β type Ⅰ receptor (TβR-I), which is involved in signals transmission of transforming growth factor beta (TGF-β), participates in cell proliferation and growth. In this study, we characterized a Tgfbr1 gene which encoded TβR-I from P. fucata martensii (Pmtgfbr1). Pmtgfbr1 cDNA contains an open reading frame of 1569 bp and encodes a polypeptide of 522 amino acids (aa). Pmtgfbr1 possesses a typical TβR-I structure (extracellular receptor ligand domain, transmembrane domain, and cytoplasmic tyrosine kinase catalytic domain). Pmtgfbr1 is expressed in all the studied tissues and exhibited the highest expression level in the adductor muscle. Moreover, Pmtgfbr1 exhibited the lower expression level in the larger group (L) than that in the smaller group (S) and is negatively correlated with growth traits (P
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- 2018
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21. Metabolomics Responses of Pearl Oysters (Pinctada fucata martensii) Fed a Formulated Diet Indoors and Cultured With Natural Diet Outdoors
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Chuangye Yang, Ruijuan Hao, Xiaodong Du, Yuewen Deng, Ruijiao Sun, and Qingheng Wang
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metabolomics ,formulated diet ,nutritional requirements ,GC–MS ,Pinctada fucata martensii ,Physiology ,QP1-981 - Abstract
Natural disasters and environmental pollution are the main problems in traditional offshore cultivation. While culturing pearl oysters through industrial farming can avoid these problems, food availability in this case is limited. This study compares the metabolomics responses of pearl oysters, Pinctada fucata martensii, fed a formulated diet indoors with those of oysters cultured with natural diet outdoors by using a gas chromatography time-of-flight mass spectrometry (GC-TOF/MS)-based metabolomics approach. The animals were divided into two groups as follows: the experimental group (EG) was fed a formulated diet indoors and the control group (CG) was cultured with natural diet outdoors. After 45 days of feeding, the survival rate of EG was significantly higher than that of CG. The absolute growth rate (AGR) of the total weight of EG did not significantly differ from that of CG, but the AGRs of the shell length, shell height, and shell width of CG were significantly higher than those of EG. EG showed significantly higher amylase activities than CG, and the hexokinase and glucose-6-phosphate isomerase concentrations of the former were significantly lower than those of the latter. Metabolomics revealed 125 metabolites via mass spectrum matching with a spectral similarity value > 700 in the hepatopancreas, and 48 metabolites were considered to be significantly different between groups (VIP > 1 and P < 0.05). Pathway analysis results indicated that these significantly different metabolites were involved in 34 pathways. Further integrated key metabolic pathway analysis showed that, compared with CG, EG had lower capabilities for cysteine and methionine metabolism, sulfur metabolism, and starch and sucrose metabolism. This study demonstrated that the formulated diet could be an excellent substitute for natural diet; however, its nutrients were insufficient. Effective strategies should be developed to enhance the utilization of formulated diets.
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- 2018
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22. Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii
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Qingyue Chen, Wanmei Yu, Ruijuan Hao, Jingmiao Yang, Chuangye Yang, Yuewen Deng, and Yongshan Liao
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Aquatic Science - Published
- 2022
23. Identification of Genes and SNPs Related to Body Colors by Transcriptome Profiling in Leopard Coral Grouper (Plectropomus leopardus Lacépède)
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Yucong Hong, Kaihui Sun, Xiaowen Zhu, Xufeng Zhu, Qiuxia Deng, Yang Huang, Ruijuan Hao, and Chunhua Zhu
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Aquatic Science - Abstract
Body colors are an important trait for the survival and reproduction of animals. In this study, transcriptomes and related single nucleotide polymorphisms (SNPs) of black- and red-colored Plectropomus leopardus (Lacépède) were identified to investigate important molecules and SNP markers associated with body color formation. In the comparison of red- and black-colored groups, 489 differential expressed genes (DEGs) were detected and among them, 236 up- and 253 down-regulated genes were found in the red-colored group. Gene ontology (GO) analysis of DEGs showed that pigmentation associated terms including pigment granule, pigmentation, melanin metabolic process, tyrosine metabolic process, pigment metabolic process, and pigmentation cell differentiation were enriched. Kyoto encyclopedia of genes and genomes (KEGG) analysis of DEGs presented that melanin related pathways (tyrosine metabolism and melanogenesis) were enriched. Furthermore, lipid metabolism and immune-related metabolism were also enriched. A total of 1,048,575 SNPs were detected from the transcriptome, and among them, 627,172 SNPs were located in the gene region. A total of 1323 SNPs were identified as differential SNPs in the comparison of black- and red-colored groups and were located in 1127 genes. Among the 1127 genes, 20 genes were identified as DEGs and were comprised of 9 SNPs with a potential role in body color selective breeding in fish farming.
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- 2023
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24. Genome-wide association study analysis to resolve the key regulatory mechanism of biomineralization in Pinctada fucata martensii
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Zhe Zheng, Ruijuan Hao, Chuangye Yang, Yu Jiao, Qingheng Wang, Ronglian Huang, Yongshan Liao, Jianbo Jian, Yao Ming, Lixin Yin, Weiming He, Ziman Wang, Chuyi Li, Qi He, Kun Chen, Yuewen Deng, and Xiaodong Du
- Subjects
Genetics ,Ecology, Evolution, Behavior and Systematics ,Biotechnology - Abstract
Biomineralization-controlled exo-/endoskeleton growth contributes to body growth and body size diversity. Molluscan shells undergo ectopic biomineralization to form the exoskeleton and biocalcified "pearl" involved in invading defence. Notably, exo-/endoskeletons have a common ancestral origin, but their regulation and body growth are largely unknown. This study employed the pearl oyster, Pinctada fucata marntensii, a widely used experimental model for biomineralization in invertebrates, to perform whole-genome resequencing of 878 individuals from wild and breeding populations. This study characterized the genetic architecture of biomineralization-controlled growth and ectopic biomineralization. The insulin-like growth factor (IGF) endocrine signal interacted with ancient single-copy transcription factors to form the regulatory network. Moreover, the "cross-phylum" regulation of key long noncoding RNA (lncRNA) in bivalves and mammals indicated the conserved genetic and epigenetic regulation in exo-/endoskeleton growth. Thyroid hormone signal and apoptosis regulation in pearl oysters affected ectopic biomineralization in pearl oyster. These findings provide insights into the mechanism underlying the evolution and regulation of biomineralization in exo-/endoskeleton animals and ectopic biomineralization.
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- 2022
25. Effect of vitamin D3 on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii after transplantation: Insights from LC–MS-based metabolomics analysis
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Xiaodong Du, Ruijiao Sun, Chuangye Yang, Yuewen Deng, Qingheng Wang, and Ruijuan Hao
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0301 basic medicine ,Vitamin ,Pinctada fucata martensii ,Aquaculture ,Aquatic Science ,engineering.material ,Biology ,Antioxidants ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,Metabolomics ,Animal science ,Liquid chromatography–mass spectrometry ,Immunity ,Animals ,Environmental Chemistry ,Pinctada ,Cholecalciferol ,Dose-Response Relationship, Drug ,Pearl oyster ,04 agricultural and veterinary sciences ,General Medicine ,Immunity, Innate ,Transplantation ,030104 developmental biology ,chemistry ,Metabolome ,040102 fisheries ,engineering ,0401 agriculture, forestry, and fisheries ,Pearl ,Chromatography, Liquid - Abstract
Postoperative care is a critical step of pearl culture that ultimately determines culture success. To determine the effect of dietary vitamin D3 (VD3) levels on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii during postoperative care and explore the mechanisms behind this phenomenon, five isonitrogenous and isolipidic experimental diets were formulated by adding different levels of dietary VD3 (0, 500, 1000, 3000, and 10000 IU/kg), and the diets were fed to five experimental groups (EG1, EG2, EG3, EG4, and EG5) in turn and cultured indoors. The control group (CG) was cultured in the natural sea. Pearl oysters that were 1.5 years old were subjected to nucleus insertion. After culturing for 30 days, EG3 exhibited significantly higher survival rates than those in CG and EG5 (P 0.05). Moreover, EG3 exhibited the highest activities of alkaline phosphatase, acid phosphatase, catalase, superoxide dismutase, and lysozyme. However, EG5 achieved the highest activities of glutathione peroxidase. Metabolomics-based profiling of pearl oysters fed with high levels of dietary VD3 (EG5) and optimum levels of dietary VD3 (EG3) revealed 76 significantly differential metabolites (SDMs) (VIP 1 and P 0.05). Pathway analysis indicated that SDMs were involved in 21 pathways. Furthermore, integrated key metabolic pathway analysis suggested that pearl oysters in EG5 regulated the pentose phosphate pathway, glutathione metabolism, sphingolipid metabolism, and arachidonic acid metabolism in response to stress generated from excessive VD3. These findings had significant implications on strengthening the future development and application of VD3 in aquaculture of pearl oyster P. f. martensii.
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- 2019
26. Dietary astaxanthin improves the antioxidant capacity, immunity and disease resistance of coral trout (Plectropomus leopardus)
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Xiaowen Zhu, Ruijuan Hao, Junpeng Zhang, Changxu Tian, Yucong Hong, Chunhua Zhu, and Guangli Li
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Trout ,Dietary Supplements ,Environmental Chemistry ,Animals ,General Medicine ,Aquatic Science ,Xanthophylls ,Anthozoa ,Animal Feed ,Antioxidants ,Immunity, Innate ,Diet ,Disease Resistance - Abstract
The effects of astaxanthin on growth performance, digestive enzyme activity, antioxidant capacity, immune ability, resistance to Vibrio harveyi infection of coral trout (Plectropomus leopardus, initial weight 17.44 ± 0.05 g) were studied by 8-week feeding trial. Four iso-nitrogenous and iso-lipidic experimental diets containing astaxanthin 0 (A0), 0.05 (A1), 0.1 (A2) and 0.2 (A3) g/kg were formulated with the addition of Haematococcus pluvialis powder (astaxanthin content accounts for 100 g/kg) of 0, 0.5, 1.0 and 2.0 g/kg, separately. The feeding experiment lasted for 56 days, and it was found that supplementing the diet with astaxanthin-rich H. pluvialis powder had no significant impact on the growth performance about coral trout (P 0.05). Compared with the A0 group, the activities of amylase, lipase, and trypsin in the liver of the A2 group was dramatically increased (P 0.05); catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities and total antioxidant capacity (T-AOC) level in serum and liver were dramatically higher in the A2 group before as well as after the challenge (P 0.05); after the challenge, the acid phosphatase (ACP) and lysozyme (LZ) activities, and complement (C3 and C4) contents in serum and liver were significantly raised for the A2 group (P 0.05); the liver relative expressions of copper-zinc superoxide dismutase (sod-1), manganese superoxide dismutase (sod-2), cat, acp6, akp, lz-c, immunoglobulin M (igm), c3, and c4-b in the A2 group were significantly up-regulated before and after the challenge (P 0.05); the rate of survival follow V. harveyi challenge in the group A2 was dramatically higher (P 0.05). In summary, this study indicated that adding 1.0 g/kg astaxanthin-rich H. pluvialis powder (the content of astaxanthin is 0.091 g/kg) could improve the digestive enzyme activity, antioxidant capacity, immunity, and the ability to resist the challenge of V. harveyi in coral trout.
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- 2021
27. Multi-omics analysis reveals the influence of tetracycline on the growth of ryegrass root
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Tao, Han, Zhaorong, Mi, Zhe, Chen, Jinjin, Zhao, Haiguang, Zhang, Yang, Lv, Shouyang, Du, Ruifang, Bu, Junguo, Zhou, Xinzheng, Li, Zhiqiang, Sun, Zhuo, Chen, Yiting, Song, Jun, Zhang, Ruijuan, Hao, and Shidong, Ge
- Subjects
Environmental Engineering ,Health, Toxicology and Mutagenesis ,Lolium ,Metabolome ,Environmental Chemistry ,Tetracycline ,Transcriptome ,Pollution ,Waste Management and Disposal ,Anti-Bacterial Agents ,Biological Phenomena - Abstract
Environmental factors, including antibiotics such as tetracycline, can alter biological processes in plants. To ascertain how cell/tissue response to tetracycline, a multi-omic analysis was implemented to explore the molecular mechanism of tetracycline influencing the growth of ryegrass root. Tetracycline induced extensive changes in the root metabolome in plants, particularly impacting metabolites of flavonoid metabolic pathways, which were supported through consistent differences between transcriptome and proteome. Cross-comparison between mRNA and protein contents considered the authentication of congruence with related metabolites and revealed changes of several biological processes under tetracycline stress. Overall, we present an undemanding multi-omic strategy to survey the significant influence on the root under tetracycline stress.
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- 2022
28. Immune response of pearl oysters to stress and diseases
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Xiaodong Du, Ruijuan Hao, Linda Adzigbli, Yuewen Deng, Yu Jiao, Ronglian Huang, and Qingheng Wang
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Innate immune system ,Ecology ,business.industry ,Pearl oyster ,Zoology ,Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,engineering.material ,Immune system ,Aquaculture ,engineering ,business ,Pearl - Published
- 2019
29. Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Xiaodong Du, Ruijuan Hao, Xinwei Xiong, Yu Jiao, Zhe Zheng, and Yuewen Deng
- Subjects
0106 biological sciences ,lcsh:QH426-470 ,media_common.quotation_subject ,lcsh:Biotechnology ,Pinctada fucata martensii ,Shell (structure) ,Biology ,Shell formation ,01 natural sciences ,Transcriptome ,03 medical and health sciences ,Animal Shells ,lcsh:TP248.13-248.65 ,Genetics ,Animals ,Pinctada ,Metamorphosis ,030304 developmental biology ,media_common ,0303 health sciences ,Larva ,Settlement ,Molecular events ,Pearl oyster ,Gene Expression Profiling ,Metamorphosis, Biological ,Genomics ,Pacific oyster ,biology.organism_classification ,Neurosecretory Systems ,Cell biology ,Extracellular Matrix ,lcsh:Genetics ,Gene Ontology ,Crassostrea ,010606 plant biology & botany ,Biotechnology ,Research Article - Abstract
Background Marine bivalves undergo complex development processes, such as shell morphology conversion and changes of anatomy and life habits. In this study, the transcriptomes of pearl oyster Pinctada fucata martensii and Pacific oyster Crassostrea gigas at different development stages were analyzed to determine the key molecular events related to shell formation, settlement and metamorphosis. Result According to the shell matrix proteome, biomineralization-related genes exhibited a consensus expression model with the critical stages of shell formation. Differential expression analysis of P. f. martensii, revealed the negative regulation and feedback of extracellular matrixs as well as growth factor pathways involved in shell formation of larvae, similar to that in C. gigas. Furthermore, neuroendocrine pathways in hormone receptors, neurotransmitters and neuropeptide receptors were involved in shell formation, settlement and metamorphosis. Conclusion Our research demonstrated the main clusters of regulation elements related to shell formation, settlement and metamorphosis. The regulation of shell formation and metamorphosis could be coupled forming the neuroendocrine-biomineralization crosstalk in metamorphosis. These findings could provide new insights into the regulation in bivalve development. Electronic supplementary material The online version of this article (10.1186/s12864-019-5505-8) contains supplementary material, which is available to authorized users.
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- 2019
30. Molecular and functional analysis of PmCHST1b in nacre formation of Pinctada fucata martensii
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Ruijuan Hao, Xiaodong Du, Ronglian Huang, Zhe Zheng, Qingheng Wang, and Yuewen Deng
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Models, Molecular ,0301 basic medicine ,Sulfotransferase ,Physiology ,Keratan sulfate ,Biology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Protein Domains ,RNA interference ,Animals ,Amino Acid Sequence ,Pinctada ,Sulfate ,Nacre ,Molecular Biology ,Gene ,Peptide sequence ,Phylogeny ,Minerals ,Base Sequence ,030102 biochemistry & molecular biology ,biology.organism_classification ,030104 developmental biology ,chemistry ,Keratan Sulfate ,Trochophore ,Sulfotransferases ,Biomineralization - Abstract
Keratan sulfate possesses considerable amounts of negatively charged sulfonic acid groups and participates in biomineralization. In the present study, we investigated characteristics and functions of a CHST1 gene identified from the pearl oyster Pinctada fucata martensii (PmCHST1b) which participated in the synthesis of keratan sulfate. PmCHST1b amino acid sequence carried a typical sulfotransferase-3 domain (sulfotransfer-3 domain) and belonged to membrane-associated sulfotransferases. Homologous analysis of CHST1 from different species showed the conserved motif (5' PSB motif and 3' PB motif) which interacted with 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Structure analysis of sulfotransferase domain indicted that PmCHST1b showed the conserved catalytic structure character and the relationships presented in the phylogenetic tree conformed to that of traditional taxonomy. Expression pattern of PmCHST1b in different tissues and development stages showed that PmCHST1b widely expressed in all the detected tissues and development stages and showed the highest expression level in the central zone of mantle (MC). PmCHST1b expressed highly in the trochophore, D-stage larvae and spat which corresponded to prodissoconch and dissoconch shell formation, respectively. RNA interference (RNAi) successfully inhibited expression level of PmCHST1b in MC (P
- Published
- 2018
31. The differences in the nitrogen isotope composition between a maize hybrid and its parents
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Ruijuan Hao, Zhoufeng Wang, and Xiangzhong Li
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China ,010504 meteorology & atmospheric sciences ,Heterosis ,0207 environmental engineering ,chemistry.chemical_element ,02 engineering and technology ,Biology ,01 natural sciences ,Crossbreed ,Plant Roots ,Zea mays ,Inorganic Chemistry ,Hybrid Vigor ,Environmental Chemistry ,020701 environmental engineering ,0105 earth and related environmental sciences ,General Environmental Science ,Hybrid ,Isotope ,Nitrogen Isotopes ,Plant Stems ,Stable isotope ratio ,Chimera ,Nitrogen ,Isotopes of nitrogen ,Plant Leaves ,Agronomy ,chemistry ,Composition (visual arts) - Abstract
Understanding of nitrogen stable isotope variation in maize hybrids might help obtaining information on nitrogen absorption and distribution in different maize hybrids. In this study, we examined the nitrogen isotopic composition of different parts of maize hybrids under a laboratory culture experiment. The results showed that the δ15N values of different parts of the maize hybrid and its parents were ordered as follows: δ15Nstem>δ15Nleaf>δ15Nroot. The variation pattern of δ15N between the roots and leaves(Δδ15Nroot-leaf) of the maize hybrid was the same as that of δ15N between the roots and stems (Δδ15Nroot-stem). Therefore, the order of Δδ15Nroot-leaf as well as Δδ15Nroot-stem was as follows: Δδ15Nroot-leaf of the maize hybrid>Δδ15Nroot-leaf of the female parent (T4)>Δδ15Nroot-leaf of the male parent (803) and Δδ15Nroot-stem of the maize hybrid>Δδ15Nroot-stem of the female parent (T4)>Δδ15Nroot-stem of the male parent (803). This order is consistent with heterosis, indicating that differences in δ15N reflect the phenomenon of heterosis. The present study provides data in support of using the isotope technique to determine nitrogen distributions inside a plant and guide crossbreeding.
- Published
- 2021
32. Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii
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Chuchu Mo, Chuangye Yang, Ruijuan Hao, Yuewen Deng, Qingheng Wang, and Linda Adzigbli
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Candidate gene ,SNP ,Ocean Engineering ,Single-nucleotide polymorphism ,FGF18 ,Molecular cloning ,Biology ,03 medical and health sciences ,lcsh:Oceanography ,lcsh:VM1-989 ,lcsh:GC1-1581 ,Gene ,030304 developmental biology ,Water Science and Technology ,Civil and Structural Engineering ,Genetic association ,Genetics ,0303 health sciences ,lcsh:Naval architecture. Shipbuilding. Marine engineering ,Promoter ,04 agricultural and veterinary sciences ,Pinctada fucata martensii ,Open reading frame ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,growth traits - Abstract
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii (PmFGF18) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A >, C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits.
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- 2020
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33. Integrated analysis of the role of miRNA-mRNA in determining different body colors of leopard coral grouper (Plectropomus leopardus)
- Author
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Ruijuan Hao, Yang Huang, Mouyan Jiang, Chunhua Zhu, Xiaowen Zhu, and Changxu Tian
- Subjects
Genetics ,Messenger RNA ,biology ,Plectropomus ,Coral ,Gene expression ,microRNA ,Grouper ,Epigenetics ,Aquatic Science ,biology.organism_classification ,Function (biology) - Abstract
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate gene expression at the post-transcriptional level and play important roles in many biological processes, including controlling body color differentiation in many different animals. However, the mechanism by which specific miRNAs regulate body color formation in leopard coral groupers has remained largely elusive. In the present study, we sequenced the miRNAs of red and black skin samples from leopard coral grouper (Plectropomus leopardus) with the Illumina HiSeq X_Ten platform to explore the function of miRNAs in the body color formation. A total of 15.21 M and 13.69 M high quality reads were obtained from the black and red skin samples, respectively. Within these sequences, 626 known and 354 novel miRNAs were identified. When comparing across the two groups, we found 60 differentially expressed miRNAs, with red-colored group having 44 up-regulated miRNAs including miR-215, miR-2188, miR-194, miR-122, novel-m0118, and 16 down-regulated miRNAs including miR-187, novel-m0189, novel-m0145, compared with black-colored group (p 1). Integrated analysis of differentially expressed miRNAs and mRNAs identified 846 miRNA-mRNA pairs with opposite expression patterns. Go terms and KEGG pathway enrichment analyses showed that these putative target mRNAs were enriched for functions involving in endocrine systems, immune response, and lipid metabolism. The present study shows the potential role of miRNAs in the body color formation of fish and provides a molecular foundation to explain the role of epigenetics in fish body color selections.
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- 2022
34. Characterization of cyclin dependent kinase-7 and its differential response to grafting challenge in the black shell colored selected line of pearl oyster Pinctada fucata martensii
- Author
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Linda Adzigbli, Zihan Zhao, Chuangye Yang, Yuewen Deng, Ruijuan Hao, and Ziman Wang
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0301 basic medicine ,DNA, Complementary ,Cellular differentiation ,Aquaculture ,Aquatic Science ,Biology ,03 medical and health sciences ,Complementary DNA ,Environmental Chemistry ,Animals ,Amino Acid Sequence ,Pinctada ,Peptide sequence ,Base Sequence ,Kinase ,DNA replication ,04 agricultural and veterinary sciences ,General Medicine ,Cell cycle ,Cyclin-Dependent Kinases ,Cell biology ,Open reading frame ,030104 developmental biology ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Cyclin-dependent kinase 7 ,Sequence Alignment - Abstract
Cyclin dependent kinase-7 (Cdk-7) is a protein kinase associated with regulating the cell cycle, cell differentiation and proliferation, apoptosis and inflammatory response. This study characterized the full cDNA sequence of Cdk-7 in Pinctada fucata martensii (PmCdk-7). A full length sequence of 1473bp with an open reading frame (ORF) of 915bp and encodes a 304aa, 5′-UTR of 58bp and a 3′-UTR of 500bp was obtained. The construed amino acid sequence of PmCdk-7 comprised of a Serine/Threonine protein kinases, catalytic (S_TKc) domain with a protein kinases ATP-binding region signature (14-38aa) and the serine/Threonine protein kinases active-site signature (129-141aa) within the domain. Tissue distribution analysis revealed a high relative mRNA expression of PmCdk-7 within haemocytes. Following the insertion operation (grafting), the relative expression levels of PmCdk-7 in the haemocyte was expressed differentially among the studied groups; the black shell colored selected line (BS) and the control group (CG). High expression was recorded between 12 h and 5d with a peak at 3d suggesting a heightened level of DNA replication and inflammatory response during the pearl-sac formation and this expression was higher in BS than CS showcasing, the heightened immune capacity of BS to grafting operation. Immune stimulation experiment with bacterial endotoxin and a viral mimic revealed PmCdk-7 response to pathogenic stress. The results from our study showed that PmCdk-7 performs a vital function during the cell cycle by aiding DNA replication and also aid response to inflammations generated due to the incision from the grafting operation and long exposure to immune-stimulants (pathogens).
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- 2020
35. Molecular and functional analysis of PmC1qDC in nacre formation of Pinctada fucata martensii
- Author
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Xiaodong Du, Ruijuan Hao, Zhe Zheng, Qi He, and Bingyi Xie
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0301 basic medicine ,Pinctada fucata martensii ,Aquatic Science ,Biology ,03 medical and health sciences ,Protein sequencing ,Protein Domains ,RNA interference ,Animal Shells ,Environmental Chemistry ,Animals ,Amino Acid Sequence ,Pinctada ,Mantle (mollusc) ,Nacre ,Protein secondary structure ,Base Sequence ,Pearl oyster ,Complement C1q ,04 agricultural and veterinary sciences ,General Medicine ,Cell biology ,030104 developmental biology ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,RNA Interference ,High homology ,Biomineralization - Abstract
The C1q-domain-containing (C1qDC) proteins are a family of proteins characterized by a globular C1q (gC1q) domain in their C-terminus which hold the potential function in the shell formation as shell matrix proteins. In this study, a C1qDC protein was identified and characterized in pearl oyster (Pinctada fucata martensii) (PmC1qDC) to explore its function in nacre formation. The PmC1qDC-deduced protein sequence carried a typical globular C1q (gC1q) domain that possessed the typical 10-stranded β-sandwich fold with a jelly-roll topology common to all C1qDC family members and shared high homology with other gC1q domains. Homologous analysis of PmC1qDC presented it contained conserved secondary structure and Phe135, Phe155, Tyr166, Phe173, Tyr181, Phe183, and Phe256 amino acid residues. Expression pattern analysis showed that PmC1qDC expressed in all the detected tissues and exhibited a significantly higher expression level in nacre formation-associated tissues. After the shell notching, the expression level of PmC1qDC showed significantly up-regulation after 12 h in the central zone of mantle (MC). PmC1qDC expression significantly decreased in the MC after RNA interference (RNAi). Furthermore, disordered crystals with evident rough surface and irregular crystal tablets were observed in the nacre after RNAi. Results suggested that PmC1qDC affects the shell nacre formation, which is significant to improve the pearl production of pearl oyster.
- Published
- 2020
36. Characterization and development of SSR markers of Pinctada maxima by RNA-Seq approach
- Author
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Yuewen Deng, Ruijuan Hao, Linda Adzigbli, Ziman Wang, and Junhui Li
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Genetics ,lcsh:SH1-691 ,0303 health sciences ,Genetic diversity ,biology ,Locus (genetics) ,RNA-Seq ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,lcsh:Aquaculture. Fisheries. Angling ,Loss of heterozygosity ,03 medical and health sciences ,Pinctada maxima ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Microsatellite ,Animal Science and Zoology ,Allele ,Maxima ,030304 developmental biology - Abstract
In this study, we developed microsatellite markers, analyzed their distribution and frequency and verified polymorphism based on the transcriptome data of pearl oyster Pinctada maxima. Totally, 58,121 potential EST-SSRs from 145,877 sequences were identified. We randomly selected 100 of these primer pairs for further validation in the stock, 76 primer pairs were successfully amplified and 25 primer pairs were found to be polymorphic. The number of alleles at each locus ranged from 2 to 5, with an average allele number of 2.96. The observed heterozygosity ranged from 0.185 to 0.931 with an average of 0.656. The expected heterozygosity ranged from 0.337 to 0.754 with an average of 0.552. The polymorphic information content ranged from 0.263 to 0.690 with an average of 0.462. These markers will provide an effective tool for genetic diversity, genetic map construction and molecular-assisted breeding in the species of P. maxima. Keywords: Pinctada maxima, SSR markers, Polymorphism, Genetic diversity
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- 2019
37. Metabolomic responses of juvenile pearl oyster Pinctada maxima to different growth performances
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Qingheng Wang, Xiaodong Du, Chuangye Yang, Zhe Zheng, Ruijuan Hao, Yuewen Deng, and Ziman Wang
- Subjects
0301 basic medicine ,biology ,Metabolite ,Pearl oyster ,Zoology ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,Spectral similarity ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Metabolomics ,chemistry ,Pinctada maxima ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Juvenile ,Maxima - Abstract
Similar to other marine bivalves, Pinctada maxima shows unsynchronized growth, but the underlying metabolic mechanisms have not been studied. In this study, gold-lipped pearl oyster P. maxima from cultured stocks were selected to produce progeny stock. At 180 days, the stock was sorted by size, and fast-growing individuals and slow-growing individuals were separately sampled. Then, a metabolomic approach based on gas chromatography–mass spectroscopy was applied to assess the metabolite changes between the fast-growing and slow-growing groups of P. maxima and to understand the mechanism of their unsynchronized growth. In the metabolomics assay, among the 896 peaks isolated, 111 metabolites were revealed a spectral similarity value of >700 by using mass spectrum matching, and 48 were considered as significantly different metabolites (SDMs; VIP > 1 and P
- Published
- 2018
38. GC–TOF/MS-based metabolomics studies on the effect of protein sources in formulated diet for pearl oyster Pinctada fucata martensii
- Author
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Ruijiao Sun, Qingheng Wang, Xiaodong Du, Zhe Zheng, Ruijuan Hao, Chuangye Yang, and Yuewen Deng
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,biology ,Sulfur metabolism ,Environmental pollution ,Phenylalanine ,04 agricultural and veterinary sciences ,Metabolism ,Aquatic Science ,biology.organism_classification ,Gluten ,03 medical and health sciences ,030104 developmental biology ,Metabolomics ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Hepatopancreas ,Food science ,Tyrosine - Abstract
Natural disasters and environmental pollution are the main problems for traditional offshore culture. Pearl oyster culture through industrial farming can prevent these difficulties, but food availability is limited. In this study, we formulated two diets, P3 and P5, with yeast powder and corn gluten, respectively, as major protein sources. Two experimental groups (EG1 and EG2) were separately fed with P3 and P5 diets indoors. After 45 days of feeding, GC–TOF/MS-based metabolomics of the hepatopancreas was performed to assess the effect of different formulated diets on pearl oyster (Pinctada fucata martensii) combined with growth performance. EG1 displayed significantly faster absolute growth rate and higher survival rate than EG2. In the metabolomic assay, 125 metabolites were identified by mass spectrum matching with a spectral similarity value (SV) > 700, and 35 were considered significantly different metabolites (SDMs) (VIP > 1 and P 0.1). The impact values of phenylalanine, tyrosine, and tryptophan biosynthesis, ascorbate and aldarate metabolism, phenylalanine metabolism, inositol phosphate metabolism, tyrosine metabolism, sulfur metabolism, and cysteine and methionine metabolism were 0.500, 0.500, 0.308, 0.219, 0.207, 0.167, and 0.161, respectively. Further integrated key metabolic pathway analysis showed that EG2 exhibited lower capability for inositol phosphate metabolism, ascorbate and aldarate metabolism, phenylalanine metabolism, and tyrosine metabolism. This study suggested that yeast powder is a better protein source of formulated diet for pearl oysters than corn gluten.
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- 2018
39. Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii
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Yuewen Deng, Qingheng Wang, Zhe Zheng, Ruijuan Hao, Junhui Li, and Chao Lei
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0301 basic medicine ,lcsh:Biotechnology ,food and beverages ,04 agricultural and veterinary sciences ,Biology ,Molecular cloning ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Reverse transcription polymerase chain reaction ,03 medical and health sciences ,030104 developmental biology ,Biochemistry ,lcsh:Biology (General) ,Complementary DNA ,lcsh:TP248.13-248.65 ,Botany ,Gene expression ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Carotenoid transport ,Hepatopancreas ,Peptide sequence ,lcsh:QH301-705.5 ,Biotechnology ,Pinctada - Abstract
Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P
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- 2017
40. Effects of protein sources on growth, immunity and antioxidant capacity of juvenile pearl oyster Pinctada fucata martensii
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Yongshan Liao, Yuewen Deng, Xiaodong Du, Qingheng Wang, Ruijuan Hao, Yu Jiao, Chuangye Yang, and Ruijiao Sun
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0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Soybean meal ,Aquaculture ,Aquatic Science ,Antioxidants ,Superoxide dismutase ,Random Allocation ,03 medical and health sciences ,Botany ,medicine ,Animals ,Environmental Chemistry ,Pinctada ,Food science ,Amylase ,Shellfish ,chemistry.chemical_classification ,biology ,Glutathione peroxidase ,04 agricultural and veterinary sciences ,General Medicine ,Gluten ,030104 developmental biology ,chemistry ,Catalase ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Dietary Proteins - Abstract
In this study, we formulated five diets, namely, P1, P2, P3, P4 and P5, with Chlorella sp. powder, Spirulina platensis powder, yeast powder, soybean meal and corn gluten, respectively, as major protein sources. A feeding experiment was designed to evaluate the effects of formulated diets on the growth performance, immunity and antioxidant and biomineralization capacity of juvenile pearl oyster (Pinctada fucata martensii). In the experiments, the five groups were separately fed with P1, P2, P3, P4 and P5 diets. After 45 days of feeding, pearl oysters fed on P1, P2, P3 and P4 diets showed significantly higher absolute growth rate and protease and amylase activities than those fed on P5 diet (P < 0.05). Moreover, pearl oysters fed on P1, P2, P3 and P4 diets exhibited significantly higher activities of alkaline phosphatase (AKP), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) (P < 0.05). Significantly higher expression levels of SOD, GPx, CAT, heat shock protein (HSP) 70, HSP90, nacrein, pif177 and pearlin mRNA were observed in pearl oysters fed on P1, P2, P3 and P4 diets relative to those fed on P5 (P < 0.05). Results suggested the suitability of Chlorella sp. powder, S. platensis powder, yeast powder and soybean meal as protein sources for development of formulated diets for pearl oyster P. f. martensii.
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- 2017
41. Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii
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Xiaodong Du, Yu Jiao, Qingheng Wang, Yuewen Deng, Zhe Zheng, Ruijuan Hao, Ronglian Huang, and Chuangye Yang
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0301 basic medicine ,Signal peptide ,Biomineralization ,Chondroitin sulfate ,lcsh:Biotechnology ,Biology ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,chemistry.chemical_compound ,symbols.namesake ,0302 clinical medicine ,RNA interference ,lcsh:TP248.13-248.65 ,Carbohydrate sulfotransferase 11 ,Chondroitin ,Sulfate ,Biomineral ,Gene ,lcsh:QH301-705.5 ,Mantle tissue ,Molluscs ,Golgi apparatus ,Transmembrane domain ,030104 developmental biology ,chemistry ,Biochemistry ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,symbols ,Proteoglycans ,Chondroitin-4-sulfotransferase-1 (C4ST-1) ,Biotechnology ,Real-time PCR - Abstract
Background : C4ST-1 catalyses the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes ( PmCHST11a and PmCHST11b ) which encoded the C4ST-1 and explored the functions of these genes in the synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells. Results: Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a transmembrane domain. These properties indicated that these genes localise in the Golgi apparatus. Real-time PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in extrapallial fluid. Moreover, shell nacre crystallised irregularly with a rough surface after RNA interference. Conclusions: This study indicated that PmCHST11a and PmCHST11b are involved in the nacre formation of P. fucata martensii through participating in the synthesis of chondroitin sulfate. Normal 0 false false false EN-US ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.5pt; mso-bidi-font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-font-kerning:1.0pt; mso-fareast-language:ZH-CN;}
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- 2017
42. Response of pearl oyster Pinctada fucata martensii to allograft-induced stress from lipid metabolism
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Yuewen Deng, Ruijuan Hao, Chuangye Yang, Chengzhang He, and Xiaodong Du
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0301 basic medicine ,Hemocytes ,Linoleic acid ,Vaccenic acid ,Aquatic Science ,Biology ,Palmitic acid ,03 medical and health sciences ,chemistry.chemical_compound ,Stress, Physiological ,Environmental Chemistry ,Palmitoleic acid ,Animals ,Pinctada ,chemistry.chemical_classification ,Fatty Acids ,food and beverages ,Fatty acid ,04 agricultural and veterinary sciences ,General Medicine ,Allografts ,Lipid Metabolism ,eye diseases ,Muscle, Striated ,Transplantation ,Oleic acid ,030104 developmental biology ,chemistry ,Biochemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,lipids (amino acids, peptides, and proteins) ,Heptadecanoic acid ,Transcriptome - Abstract
The pearl oyster, Pinctada fucata martensii, produces high-quality pearls. During pearl production, excess immune and inflammatory response after transplantation will lead to nucleus rejection, pearl sac formation failure, and death of the host pearl oyster. The hemocyte transcriptome and fatty acid (FA) contents in the adductor muscle before and after transplantation were analyzed to investigate the response of pearl oyster P. f. martensii to allograft-induced stress from lipid metabolism. The hemocyte transcriptome analysis detected 193 lipid metabolism-related genes, such as the elongation of very long-chain FA protein 5, acyl-CoA 6-desaturase, cytochrome P450, phospholipase A2, glycerol-3-phosphate O-acyltransferase, and prostaglandin-H2 d-isomerase. Pathway enrichment analyses revealed that these genes were mainly involved in the "biosynthesis of unsaturated FAs," "FA biosynthesis," "ARA metabolism," and "glycerolipid metabolism." An analysis of FA contents in the adductor muscle indicated no significant difference in the contents of lauric acid, myristic acid, pentadecanoic acid, palmitic acid, palmitoleic acid, heptadecanoic acid, stearic acid, oleic acid, vaccenic acid, linoleic acid, arachidic acid, α-linolenic acid, eicosadienoic acid, docosadienoic acid, and 11,14,17-eicosatrienoic acid. However, ARA, DHA, and EPA in the adductor muscle after transplantation were significantly greater than those processed without grafting surgery. These results suggest that pearl oysters require more polyunsaturated FAs (PUFAs) to regulate their inflammatory and immune response after transplantation. However, their ability to biosynthesize unsaturated FAs is limited. Given these results, the addition of PUFA-containing diets or selection of a line with strong ability to biosynthesize unsaturated FAs may be valuable for pearl oyster recovery after transplantation.
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- 2019
43. Additional file 7: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
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The ecdysone receptors and the Nvds in bivalves. a. The ecdysone receptors in mollusk species. b, the expression pattern of Ecr at different development stages in C. gigas. E, egg; TC, two cells; FC, four cells; EM, early morula; M, morula; B, blastula; RM, rotary movement; FS, free swimming;EG, early gastrula stage; G, gastrula; T1, trochophore 1; T2, trochophore 2; T3,trochophore 3; T4, trochophore 4; T5, trochophore 5; ED1, early D-larva 1; ED2, early D-larva 2; D1, D-larva 1; D2, D-larva 2; D3, D-larva 3; D4, D-larva 4; D5, D-larva 5; D6, D-larva 6; D7, D-larva 7; EU1, early umbo larva 1; EU2, early umbo larva 2; U1, umbo larva 1; U2, umbo larva 2; U3, umbo larva 3; U4, umbo larva 4; U5, umbo larva 5; U6, umbo larva 6; LU1, later umbo larva 1; LU2, later umbo larva 2; P1, pediveliger 1; P2, pediveliger 2; S, spat; and J, juvenile. c. the expression pattern of Nvd at different development stages in P. f. martensii and C. gigas. Egg, egg; Fe, fertilized egg; B, blastula; G, gastrula; ET, early trochophore; T, trochophore; D, D-stage larvae; DF, D-stage larvae before feeding; EU, early umbo larvae; EL, eyed larvae; S, spat; J, juveniles. (PDF 772 kb)
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- 2019
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44. Additional file 4: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
- Abstract
Mantle tissue specific genes of P. f. martensii (DOCX 15 kb)
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- 2019
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45. Additional file 5: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
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genetic structures - Abstract
Differentially expressed genes between early trochophore and trochophore stage involved in osteoclast differentiation in P. f. martensii. The red color represented the upregulation at trochophore stage, the green color represented the downregulation at trochophore stage. The yellow color represented the absence genes in P. f. martensii. (PDF 135 kb)
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- 2019
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46. Additional file 8: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
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animal structures ,genetic structures ,parasitic diseases ,embryonic structures ,fungi - Abstract
Expression pattern of acetylcholine receptors at different development stage in P. f. martensii. The y-axis is the normalized RPKM value. Egg, egg; Fe, fertilized egg; B, blastula; G, gastrula; ET, early trochophore; T, trochophore; D, D-stage larvae; DF, D-stage larvae before feeding; EU, early umbo larvae; EL, eyed larvae; S, spat; J, juveniles. (PDF 509 kb)
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- 2019
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47. Additional file 3: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
- Abstract
The expression pattern of shell protein coding genes at different development stage in C. gigas. The y-axis is the normalized RPKM value. E, egg; TC, two cells; FC, four cells; EM, early morula; M, morula; B, blastula; RM, rotary movement; FS, free swimming;EG, early gastrula stage; G, gastrula; T1, trochophore 1; T2, trochophore 2; T3,trochophore 3; T4, trochophore 4; T5, trochophore 5; ED1, early D-larva 1; ED2, early D-larva 2; D1, D-larva 1; D2, D-larva 2; D3, D-larva 3; D4, D-larva 4; D5, D-larva 5; D6, D-larva 6; D7, D-larva 7; EU1, early umbo larva 1; EU2, early umbo larva 2; U1, umbo larva 1; U2, umbo larva 2; U3, umbo larva 3; U4, umbo larva 4; U5, umbo larva 5; U6, umbo larva 6; LU1, later umbo larva 1; LU2, later umbo larva 2; P1, pediveliger 1; P2, pediveliger 2; S, spat; and J, juvenile. (PDF 155 kb)
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- 2019
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48. Additional file 6: of Developmental characteristics of pearl oyster Pinctada fucata martensii: insight into key molecular events related to shell formation, settlement and metamorphosis
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Zheng, Zhe, Ruijuan Hao, Xinwei Xiong, Jiao, Yu, Yuewen Deng, and Xiaodong Du
- Abstract
Expression pattern of ECMs in shell matrix at different development stages in C. gigas. The y-axis is the normalized RPKM value. E, egg; TC, two cells; FC, four cells; EM, early morula; M, morula; B, blastula; RM, rotary movement; FS, free swimming;EG, early gastrula stage; G, gastrula; T1, trochophore 1; T2, trochophore 2; T3,trochophore 3; T4, trochophore 4; T5, trochophore 5; ED1, early D-larva 1; ED2, early D-larva 2; D1, D-larva 1; D2, D-larva 2; D3, D-larva 3; D4, D-larva 4; D5, D-larva 5; D6, D-larva 6; D7, D-larva 7; EU1, early umbo larva 1; EU2, early umbo larva 2; U1, umbo larva 1; U2, umbo larva 2; U3, umbo larva 3; U4, umbo larva 4; U5, umbo larva 5; U6, umbo larva 6; LU1, later umbo larva 1; LU2, later umbo larva 2; P1, pediveliger 1; P2, pediveliger 2; S, spat; and J, juvenile. (PDF 243 kb)
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- 2019
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49. Transcriptomic analysis of differentially expressed genes in the larval settlement and metamorphosis of peanut worm Sipunculus nudus
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Yuewen Deng, Yongshan Liao, Ruzhuo Zhong, Fujun Cao, Ruijuan Hao, Chuangye Yang, and Qingheng Wang
- Subjects
larval ,Protein digestion ,media_common.quotation_subject ,Notch signaling pathway ,Aquatic Science ,Protein degradation ,lcsh:Aquaculture. Fisheries. Angling ,Transcriptome ,03 medical and health sciences ,Sipunculus nudus ,Metamorphosis ,030304 developmental biology ,media_common ,lcsh:SH1-691 ,0303 health sciences ,Larva ,biology ,fungi ,transcriptomic ,04 agricultural and veterinary sciences ,Marine invertebrates ,settlement and metamorphosis ,biology.organism_classification ,Cell biology ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Animal Science and Zoology - Abstract
Larval development, especially settlement and metamorphosis, has a vital influence on commercial culture during the life cycle of marine invertebrates. Sipunculus nudus, a peanut worm, is an important economic aquaculture species. However, information about the mechanism of its larval settlement and metamorphosis is still limited. Transcriptomic analysis was performed to determine the DEGs between the pelagospheric larva and creeping larva of S. nudus. A total of 96,455 unique unigenes were obtained and 46,355 were annotated. Transcriptome analysis revealed 19,352 DEGs, including 9,923 that were upregulated and 9,429 downregulated after settlement and metamorphosis. KEGG pathway enrichment analyses revealed that these DEGs were mainly involved in the Notch signaling pathway, TNF signaling pathway, protein digestion and absorption, thyroid hormone signaling pathway, and ECM–receptor interaction, and so on. Furthermore, the changes in genes related to cytoskeleton and cell adhesion indicated that protein degradation and apoptosis during metamorphosis mediate the loss of larval cilia and ECM remodeling functions in S. nudus metamorphosis. Changes in genes related to signal transduction and nervous system suggested the cessation of larval swimming, which is involved in settlement and metamorphosis. Changes in genes related to ingestion and digestion indicated that dietary shift occurred with metamorphic transition. Changes in some genes related to stress response and immunity indicated that conditioning induced the immune response of peanut worm. The results improve understanding of the physiological traits controlling S. nudus metamorphosis and provide a solid basis for further study.
- Published
- 2020
50. Molecular characterization of OSR1 in Pinctada fucata martensii and association of allelic variants with growth traits
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Ruijuan Hao, Chuangye Yang, Yuewen Deng, Yang Jingmiao, and Xiaodong Du
- Subjects
Genetics ,0303 health sciences ,Pinctada fucata martensii ,Single-nucleotide polymorphism ,04 agricultural and veterinary sciences ,Aquatic Science ,Biology ,biology.organism_classification ,03 medical and health sciences ,Open reading frame ,Protein sequencing ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Crassostrea ,Allele ,030304 developmental biology ,Sequence (medicine) ,Genetic association - Abstract
Odd-skipped related 1 (OSR1) from Pinctada fucata martensii (PmOSR1) was cloned in this study. PmOSR1 contained an open reading frame measuring 951 bp that encoded a polypeptide of 316 residues. Domain analysis also revealed that PmOSR1 had a typical ZnF_C2H2 domain, indicating high identity with OSR1 protein sequence from Crassostrea gigas (55.25%) and Mizuhopecten yessoensis (65.48%). The expression pattern analysis of PmOSR1 revealed its wide expression in the tissues and developmental stages. PmOSR1 also presented a higher expression in the fast-growing group and hybrid families than in the slow-growing group and inbred families. The polymorphism analysis of PmOSR1 revealed 45 single nucleotide polymorphisms (SNPs) in the genomic sequence, and association analysis presented four SNPs significantly associated with growth traits (namely, shell length, width, height, and shell weight; P
- Published
- 2020
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