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Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii
- Source :
- Electronic Journal of Biotechnology, Vol 28, Iss C, Pp 113-119 (2017)
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- Background : C4ST-1 catalyses the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes ( PmCHST11a and PmCHST11b ) which encoded the C4ST-1 and explored the functions of these genes in the synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells. Results: Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a transmembrane domain. These properties indicated that these genes localise in the Golgi apparatus. Real-time PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in extrapallial fluid. Moreover, shell nacre crystallised irregularly with a rough surface after RNA interference. Conclusions: This study indicated that PmCHST11a and PmCHST11b are involved in the nacre formation of P. fucata martensii through participating in the synthesis of chondroitin sulfate. Normal 0 false false false EN-US ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.5pt; mso-bidi-font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-font-kerning:1.0pt; mso-fareast-language:ZH-CN;}
- Subjects :
- 0301 basic medicine
Signal peptide
Biomineralization
Chondroitin sulfate
lcsh:Biotechnology
Biology
Applied Microbiology and Biotechnology
03 medical and health sciences
chemistry.chemical_compound
symbols.namesake
0302 clinical medicine
RNA interference
lcsh:TP248.13-248.65
Carbohydrate sulfotransferase 11
Chondroitin
Sulfate
Biomineral
Gene
lcsh:QH301-705.5
Mantle tissue
Molluscs
Golgi apparatus
Transmembrane domain
030104 developmental biology
chemistry
Biochemistry
lcsh:Biology (General)
030220 oncology & carcinogenesis
symbols
Proteoglycans
Chondroitin-4-sulfotransferase-1 (C4ST-1)
Biotechnology
Real-time PCR
Subjects
Details
- Language :
- English
- ISSN :
- 07173458
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Electronic Journal of Biotechnology
- Accession number :
- edsair.doi.dedup.....888876b884ea7918d5e1d19513a55cd1