69 results on '"Romanov YA"'
Search Results
2. The mechanisms for linear polarization loss of in-plane photoluminescence of InGaAs/GaAs quantum well and quantum dot structures
- Author
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Aleshkin, VY, Zvonkov, BN, Malkina, IG, Chernov, AL, Romanov, YA, and University of Groningen
- Subjects
Condensed Matter::Materials Science ,Condensed Matter::Other ,GAAS ,Physics::Optics ,GROWTH ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,LAYERS - Abstract
The mechanisms for linear polarization loss of photoluminescence from quantum wells and quantum dots in InGaAs/GaAs structures observed from cleavages have been investigated. It is found that the mechanisms for polarization loss of in-plane photoluminescence are different for OD and 2D hole states. It is shown that the potential energy asymmetry in the InGaAs layer plays an important role in the loss of the photoluminescence polarization from quantum well.
- Published
- 1999
3. Vladimir Sergeevich Imshennik (in honor of his 70th birthday)
- Author
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Nadezda Bobrova, Blinnikov, Si, Brushlinskii, Kv, Bulanov, Sv, Varshalovich, Da, Gershtein, Ss, Danilov, Mv, Domogatskii, Gv, Zabrodin, Av, Kazhdan, Ym, Nadezhin, Dk, Romanov, Ya, Sasorov, Pv, Smirnov, Vp, Shafranov, Vd, Cherepashchuk, Am, and Churazov, Md
4. [Corneal regeneration: is there a place for tissues of perinatal origin?]
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Romanov YA
- Subjects
- Pregnancy, Female, Humans, Regeneration, Cornea, Mesenchymal Stem Cells
- Abstract
The article reviews the main properties of the cornea and the mechanisms of its physiological regeneration and repair in response to damage and describes the most promising methods of treatment aimed at stimulating limbal stem cells and based on the use of native tissues of perinatal origin, umbilical cord mesenchymal stromal cells, and cell-free therapeutic products.
- Published
- 2023
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5. Mild or Moderate COVID-19 during Pregnancy Does Not Affect the Content of CD34 + Hematopoietic Stem Cells in Umbilical Cord Blood of Newborns.
- Author
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Romanov YA, Kosolapova YA, Zubkov VV, Degtyarev DN, Romanov AY, Dugina TN, and Sukhikh GT
- Subjects
- Antigens, CD34, Female, Hematopoietic Stem Cells, Humans, Pregnancy, COVID-19, Fetal Blood chemistry
- Abstract
The study included umbilical cord blood samples (n=64) intended for cryogenic storage of hematopoietic stem cells and obtained from patients with a history of mild and moderate forms of COVID-19 during pregnancy. The control group was composed of samples (n=746) obtained from healthy women in labor. A comparative analysis of the volume of cord blood collected, the total leukocyte count, the relative and absolute content of cells with the CD34
+ /CD45+ phenotype revealed no significant differences between the groups., (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2022
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6. Human Umbilical Cord Tissue-Derived Multipotent Mesenchymal Stromal Cells Exhibit Maximum Secretory Activity in the Presence of Umbilical Cord Blood Serum.
- Author
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Romanov YA, Vtorushina VV, Dugina TN, Romanov AY, Petrova NV, and Sukhikh GT
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- Animals, Cattle, Culture Media chemistry, Fetus, Gene Expression, Granulocyte Colony-Stimulating Factor genetics, Granulocyte Colony-Stimulating Factor metabolism, Granulocyte-Macrophage Colony-Stimulating Factor genetics, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Hepatocyte Growth Factor genetics, Hepatocyte Growth Factor metabolism, Humans, Interleukins genetics, Interleukins metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Primary Cell Culture, Serum Albumin, Bovine chemistry, Umbilical Cord cytology, Umbilical Cord metabolism, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Culture Media pharmacology, Fetal Blood chemistry, Mesenchymal Stem Cells drug effects, Serum Albumin, Bovine pharmacology, Umbilical Cord chemistry
- Abstract
Using multiplex analysis, we performed a comparative study of cytokine and growth factor production by human umbilical cord tissue-derived multipotent mesenchymal stromal cells (UC-MSC) cultured under standard conditions and in the presence of human umbilical cord blood serum (UCBS). It was found that the secretion of most studied molecules, including well-known inductors of regeneration HGF, G-CSF, GM-CSF, and VEGF by UCMSC considerably increased in the presence of 5% UCBS. The use of UCBS allows not only obtaining xenogenic-free cellular and cell-free therapeutic products, but also increasing the secretion of most biologically active molecules capable of stimulating repair processes.
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- 2020
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7. Human Umbilical Cord Blood Serum/Plasma: Cytokine Profile and Prospective Application in Regenerative Medicine.
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Romanov YA, Vtorushina VV, Dugina TN, Romanov AY, and Petrova NV
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- Becaplermin blood, Epidermal Growth Factor blood, Granulocyte Colony-Stimulating Factor blood, Hepatocyte Growth Factor blood, Humans, Interleukin-10 blood, Interleukin-15 blood, Interleukin-4 blood, Interleukin-5 blood, Interleukin-6 blood, Interleukin-7 blood, Cytokines blood, Fetal Blood metabolism, Intercellular Signaling Peptides and Proteins blood, Regenerative Medicine methods
- Abstract
The concentrations of cytokines and growth factors in human umbilical cord blood serum and plasma samples were measured by multiplex analysis. It was found that in comparison with peripheral blood serum of adult donors, umbilical cord blood serum and plasma contain significantly higher concentrations of the most studied molecules including IL-4, 5, 6, 7, 10 and 15, MCP-1, SCF, and SDF, as well as growth factors directly involved in the processes of regeneration (G-CSF, HGF, PDGF-BB, and VEGF). Thus, umbilical cord blood plasma and especially serum are a rich source of cytokines and growth factors with anti-inflammatory, anti-apoptotic, and angiogenic effects and can be used in various fields of regenerative medicine.
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- 2019
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8. Effect of Storage Conditions on the Integrity of Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles.
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Romanov YA, Volgina NE, Dugina TN, Kabaeva NV, and Sukhikh GT
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- Cell Differentiation physiology, Cell Proliferation physiology, Cell-Derived Microparticles metabolism, Cells, Cultured, Cryoprotective Agents, Culture Media, Conditioned, Freezing, Humans, Mesenchymal Stem Cells cytology, Umbilical Cord cytology
- Abstract
We studied the effect of storage conditions on the safety of microvesicles produced by human multipotent umbilical cord mesenchymal stromal cells into the conditioned medium. It was found that microvesicles can be stored without serious degradation for up to 1 week at 4°С, but were almost completely destroyed during freezing and thawing cycles irrespective of the storage temperatures (-20°С, -70°С, or -196°С). Similar results were obtained for lyophilized medium conditioned by human multipotent umbilical cord mesenchymal stromal cells. Addition of a cryoprotectant (5-10% DMSO) followed by freezing and/or lyophilization preserved microvesicles at a nearly initial level. These findings indicate that during storage, microvesicles, being membrane structures, behave similar to living cells and require appropriate conditions for prolonged storage.
- Published
- 2019
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9. Comparative Analysis of Secretome of Human Umbilical Cord- and Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells.
- Author
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Romanov YA, Volgina NE, Vtorushina VV, Romanov AY, Dugina TN, Kabaeva NV, and Sukhikh GT
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- Cell Differentiation physiology, Cell Proliferation physiology, Cells, Cultured, Female, Humans, Pregnancy, Bone Marrow Cells cytology, Mesenchymal Stem Cells cytology, Umbilical Cord cytology
- Abstract
Production of cytokines and growth factors by cultured human umbilical cord tissue- and bone marrow-derived multipotent mesenchymal stromal cells was measured by multiplex analysis. In most cases, the concentrations of bioactive factors in the culture medium conditioned by umbilical cord-derived cells was ten- to hundred-times higher than in the medium conditioned by bone marrow-derived cells. These results suggest that both multipotent mesenchymal stromal cells from the umbilical cord and cell-free products can have more pronounced therapeutic effect in comparison with mesenchymal stromal cells obtained from "adult" sources.
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- 2019
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10. Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles Express Surface Markers Identical to the Phenotype of Parental Cells.
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Romanov YA, Volgina NE, Dugina TN, Kabaeva NV, and Sukhikh GT
- Subjects
- Cell Differentiation physiology, Cell-Derived Microparticles chemistry, Cells, Cultured, Culture Media, Conditioned, Female, Flow Cytometry, Humans, Cell-Derived Microparticles metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Umbilical Cord cytology
- Abstract
Production of microvesicles in culture of human umbilical cord multipotent mesenchymal stromal cells was studied and comparative analysis of the expression of some surface molecules (clusters of differentiation, CD) was performed. It was found that the mesenchymal stromal cells produce microvesicles in the amount sufficient for their detection by flow cytometry. Parallel analysis of the phenotypes of maternal mesenchymal stromal cells and secreted microvesicles revealed identical expression of surface molecules CD13, CD29, CD44, CD54, CD71, CD73, CD90, CD105, CD106, and HLA-I. The concentration of microvesicles in the conditioned medium was 17.9±4.6×10
6 /ml; i.e. one cell produced ~40-50 (44.7±11.5) microvesicles over 2 days in culture.- Published
- 2018
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11. Human Umbilical Cord Mesenchymal Stromal Cells Support Viability of Umbilical Cord Blood Hematopoietic Stem Cells but not the "Stemness" of Their Progeny in Co-Culture.
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Romanov YA, Volgina NE, Balashova EE, Kabaeva NV, Dugina TN, and Sukhikh GT
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- AC133 Antigen metabolism, Antigens, CD34 metabolism, Cell Proliferation physiology, Cells, Cultured, Hematopoietic Stem Cells physiology, Humans, Mesenchymal Stem Cells physiology, Coculture Techniques methods, Fetal Blood cytology, Hematopoietic Stem Cells cytology, Mesenchymal Stem Cells cytology, Umbilical Cord cytology
- Abstract
Cell-cell interactions and the ability of mesenchymal stromal cells to support the expansion of hematopoietic progenitor cells were studied in co-culture of human umbilical cord tissue-derived mesenchymal stromal cells and nucleated umbilical cord blood cells. It was found that hematopoietic stem cells from the umbilical cord blood are capable to adhere to mesenchymal stromal cells and proliferate during 3-4 weeks in co-culture. However, despite the formation of hematopoietic foci and accumulation of CD34
+ and CD133+ cells in the adherent cell fraction, the ability of newly generated blood cells to form colonies in semi-solid culture medium was appreciably reduced. These findings suggest that human umbilical cord tissue-derived mesenchymal stromal cells display a weak capability to support the "stemness" of hematopoietic stem cell progeny despite long-term maintenance of their viability and proliferation.- Published
- 2017
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12. Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells.
- Author
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Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, and Sukhikh GT
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- Adipocytes cytology, Adipocytes metabolism, Animals, Antigens, CD genetics, Antigens, CD metabolism, Biomarkers metabolism, Cattle, Cell Differentiation drug effects, Cell Proliferation drug effects, Cells, Cultured, Culture Media chemistry, Fetal Blood chemistry, Flow Cytometry, Humans, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Osteoblasts cytology, Osteoblasts metabolism, Adipocytes drug effects, Cell Culture Techniques, Culture Media pharmacology, Mesenchymal Stem Cells drug effects, Osteoblasts drug effects, Serum chemistry
- Abstract
Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.
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- 2017
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13. Expression of Surface Molecules in Human Mesenchymal Stromal Cells Co-Cultured with Nucleated Umbilical Cord Blood Cells.
- Author
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Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, and Sukhikh GT
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- Antigens, CD genetics, Antigens, CD metabolism, Blood Cells cytology, Bone Marrow Cells cytology, Cell Adhesion, Cell Differentiation, Cell Nucleus ultrastructure, Cell Separation methods, Coculture Techniques, Fetal Blood cytology, Flow Cytometry, HLA-DR Antigens genetics, HLA-DR Antigens metabolism, Humans, Immunophenotyping, Intercellular Adhesion Molecule-1 genetics, Intercellular Adhesion Molecule-1 metabolism, Mesenchymal Stem Cells cytology, Primary Cell Culture, Vascular Cell Adhesion Molecule-1 genetics, Vascular Cell Adhesion Molecule-1 metabolism, Blood Cells metabolism, Bone Marrow Cells metabolism, Fetal Blood metabolism, Gene Expression, Mesenchymal Stem Cells metabolism
- Abstract
We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell-cell contacts with leukocytes and only for bone marrow derived cells.
- Published
- 2017
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14. Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue.
- Author
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Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, and Sukhikh GT
- Subjects
- Cell Differentiation, Cell Proliferation, Cells, Cultured, Female, Humans, Mesenchymal Stem Cell Transplantation, Cell Culture Techniques, Cryopreservation methods, Mesenchymal Stem Cells cytology, Umbilical Cord cytology
- Abstract
Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies.
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- 2016
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15. Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles.
- Author
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Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, and Sukhikh GT
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- Humans, Umbilical Cord cytology, Cell Survival, Cryopreservation, Fetal Blood cytology, Fetal Blood physiology, Hematopoietic Stem Cells cytology, Hematopoietic Stem Cells physiology
- Abstract
We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
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- 2016
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16. Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord.
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Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, and Sukhikh GT
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- Adipogenesis, Cell Division, Cells, Cultured, Collagenases, Humans, Infant, Newborn, Osteogenesis, Plastics, Stainless Steel, Cell Separation methods, Mesenchymal Stem Cells cytology, Pluripotent Stem Cells cytology, Stromal Cells cytology, Umbilical Cord cytology
- Abstract
Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity.
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- 2015
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17. Human allogeneic AB0/Rh-identical umbilical cord blood cells in the treatment of juvenile patients with cerebral palsy.
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Romanov YA, Tarakanov OP, Radaev SM, Dugina TN, Ryaskina SS, Darevskaya AN, Morozova YV, Khachatryan WA, Lebedev KE, Zotova NS, Burkova AS, Sukhikh GT, and Smirnov VN
- Subjects
- ABO Blood-Group System immunology, Child, Child, Preschool, Female, HLA Antigens immunology, Humans, Infant, Infusions, Intravenous, Male, Rh-Hr Blood-Group System immunology, Transplantation, Homologous, Cell- and Tissue-Based Therapy methods, Cerebral Palsy therapy, Cord Blood Stem Cell Transplantation methods, Fetal Blood transplantation
- Abstract
Background Aims: The term "cerebral palsy" (CP) encompasses many syndromes that emerge from brain damage at early stages of ontogenesis and manifest as the inability to retain a normal body position or perform controlled movements. Existing methods of CP treatment, including various rehabilitation strategies and surgical and pharmacological interventions, are mostly palliative, and there is no specific therapy focused on restoring injured brain function., Methods: During a post-registration clinical investigation, the safety and efficacy of intravenous infusion of allogeneic human leukocyte antigen (HLA)-unmatched umbilical cord blood (UCB) cells were studied in 80 pediatric patients with cerebral palsy and associated neurological complications. Patients received up to 6 intravenous infusions of AB0/Rh-identical, red blood cell-depleted UCB cells at an average dose of 250 × 10(6) viable cells per infusion., Results: Patients were followed for 3-36 months, and multiple cell infusions did not cause any adverse effects. In contrast, in most patients who received four or more UCB cell infusions, positive dynamics related to significant improvements in neurological status and/or cognitive functions were observed., Conclusions: The results confirm that multiple intravenous infusions of allogeneic AB0/Rh-identical UCB cells may be a safe and effective procedure and could be included in treatment and rehabilitation programs for juvenile patients with cerebral palsy., (Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
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18. Human adipose-tissue derived stromal cells in combination with hypoxia effectively support ex vivo expansion of cord blood haematopoietic progenitors.
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Andreeva ER, Andrianova IV, Sotnezova EV, Buravkov SV, Bobyleva PI, Romanov YA, and Buravkova LB
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- Adipose Tissue cytology, Cell Adhesion, Cell Hypoxia, Cell Proliferation, Coculture Techniques, Hematopoietic Stem Cells cytology, Humans, Mesenchymal Stem Cells cytology, Fetal Blood cytology, Hematopoietic Stem Cells metabolism, Mesenchymal Stem Cells metabolism, Oxygen metabolism
- Abstract
The optimisation of haematopoietic stem and progenitor cell expansion is on demand in modern cell therapy. In this work, haematopoietic stem/progenitor cells (HSPCs) have been selected from unmanipulated cord blood mononuclear cells (cbMNCs) due to adhesion to human adipose-tissue derived stromal cells (ASCs) under standard (20%) and tissue-related (5%) oxygen. ASCs efficiently maintained viability and supported further HSPC expansion at 20% and 5% O2. During co-culture with ASCs, a new floating population of differently committed HSPCs (HSPCs-1) grew. This suspension was enriched with СD34+ cells up to 6 (20% O2) and 8 (5% O2) times. Functional analysis of HSPCs-1 revealed cobble-stone area forming cells (CAFCs) and lineage-restricted colony-forming cells (CFCs). The number of CFCs was 1.6 times higher at tissue-related O2, than in standard cultivation (20% O2). This increase was related to a rise in the number of multipotent precursors - BFU-E, CFU-GEMM and CFU-GM. These changes were at least partly ensured by the increased concentration of MCP-1 and IL-8 at 5% O2. In summary, our data demonstrated that human ASCs enables the selection of functionally active HSPCs from unfractionated cbMNCs, the further expansion of which without exogenous cytokines provides enrichment with CD34+ cells. ASCs efficiently support the viability and proliferation of cord blood haematopoietic progenitors of different commitment at standard and tissue-related O2 levels at the expense of direct and paracrine cell-to-cell interactions.
- Published
- 2015
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19. Erratum to: umbilical cord blood for autologous transfusion in the early postnatal ontogeny: analysis of cell composition and viability during long-term culturing.
- Author
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Romanov YA, Balashova EE, Bystrykh OA, Titkov KV, Dugina TN, Kabaeva NV, Fedorova TA, Rogachevskii OV, Degtyarev DN, and Sukhikh GT
- Published
- 2015
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20. Umbilical cord blood for autologous transfusion in the early postnatal ontogeny: analysis of cell composition and viability during long-term culturing.
- Author
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Romanov YA, Balashova EE, Bystrykh OA, Titkov KV, Dugina TN, Kabaeva NV, Fedorova TA, Rogachevskii OV, Degtyarev DN, and Sukhikh GT
- Subjects
- Flow Cytometry, Humans, Infant, Newborn, Blood Cells physiology, Blood Specimen Collection methods, Cell Culture Techniques methods, Cell Survival physiology, Fetal Blood cytology, Fetal Blood transplantation, Hematopoietic Stem Cells physiology
- Abstract
Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of storage [corrected] and inability of cells to colony formation after 2 weeks.
- Published
- 2015
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21. [EFFECT OF STROMAL CELLS AND OXYGEN CONCENTRATION ON THE MAINTENANCE OF CORD BLOOD HEMATOPOIETIC PRECURSORS].
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Sotnezova EV, Gornostaeva AN, Andreeva ER, Romanov YA, Balashova EE, and Buravkova LB
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- Coculture Techniques, Fetal Blood chemistry, Hematopoietic Stem Cells cytology, Humans, Mesenchymal Stem Cells cytology, Oxygen metabolism, Fetal Blood metabolism, Hematopoietic Stem Cells metabolism, Mesenchymal Stem Cells metabolism, Oxygen pharmacology
- Abstract
The paper analyses the morphological and functional features of cord blood cells (CBCs) in the co-culture with multipotent mesenchymal stromal cells (MMSCs) from human adipose tissue under tissue-related oxygen. We have established that MMSCs effectively maintained viability of CBCs at different oxygen concentrations (1, 5 and 20%). According to the data obtained, the oxygen concentration affected the number of colony-forming units (CFUs) formed by CBCs in selective medium. In particular, not co-cultured CBCs the 1 and 5% O2 than at 20% O2. After co-culture with MMSCs, the CFUs numbers were similar at 1 and 20% O2 and increased by 30 at 5% O2. Tissue related O2 concentrations had an impact on the proportion of lineage-resctricted CFCs among CBCs: the number of more committed progenitors--CFU-G and CFU-M, increased, and multi and bipotent--CFU-GEMM and CFU-GM, decreased at low oxygen concentrations. This effect was more pronounced after co-culture compared to that of initial CBCs. Thus, the presence of stromal cells and tissue-related oxygen jointly and severally influenced CBCs in vitro.
- Published
- 2015
22. Enrichment of umbilical cord blood mononuclears with hemopoietic precursors in co-culture with mesenchymal stromal cells from human adipose tissue.
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Maslova EV, Andreeva ER, Andrianova IV, Bobyleva PI, Romanov YA, Kabaeva NV, Balashova EE, Ryaskina SS, Dugina TN, and Buravkova LB
- Subjects
- Adipose Tissue cytology, Cell Proliferation, Cell Separation, Cell Survival, Cells, Cultured, Coculture Techniques, Feeder Cells physiology, Female, Humans, Fetal Blood cytology, Hematopoietic Stem Cells physiology, Mesenchymal Stem Cells physiology
- Abstract
We demonstrated the possibility of enrichment of umbilical cord blood mononuclear fraction with early non-differentiated precursors under conditions of co-culturing with mesenchymal stromal cells from the human adipose tissue. It was established that umbilical cord blood mononuclear cells adhered to mesenchymal stromal cell feeder and then proliferate and differentiate into hemopoietic cells. In comparison with the initial umbilical cord blood mononuclear fraction, the cell population obtained after 7-day expansion contained 2-fold more CFU and 33.4 ± 9.5 and 24.2 ± 11.2% CD34(+) and CD133(+) cells, respectively, which corresponds to enrichment of precursor cell population by 148 ± 60. The proposed scheme of expansion of hemopoietic cells from umbilical cord blood is economically expedient and can widely used in biology and medicine.
- Published
- 2014
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23. Rhythms of cell division of different periodicity in small intestinal cryptic epithelium and their contribution to circadian rhythm formation.
- Author
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Antokhin AI, Zharkova NA, Zakharchenko AV, and Romanov YA
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- Animals, Epithelial Cells cytology, Fourier Analysis, Intestinal Mucosa cytology, Intestine, Small cytology, Male, Mice, Mitosis physiology, Mitotic Index, Time Factors, Cell Division physiology, Circadian Rhythm physiology, Epithelial Cells physiology, Intestinal Mucosa physiology, Intestine, Small physiology
- Abstract
We used an improved method of chronobiological information processing enabling not only to detect oscillations with different frequencies, but also to determine the significance of each harmonic. This has made it possible to identify significant high-power harmonics present in the majority of cell positions in the crypt. These harmonics make the major contribution to the formation of diurnal rhythm of cell division in the crypt and hence determine spatial and temporal organization of the proliferative system in the crypt.
- Published
- 2012
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24. Zener tunneling in semiconductor superlattices.
- Author
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Romanova JY, Demidov EV, Mourokh LG, and Romanov YA
- Abstract
Characteristics of miniband tunneling and Wannier-Stark levels in semiconductor superlattices are studied as regards their dependence on the symmetry of the unit cells and the type of miniband structure. We modify the k ⋅ p method into a k ⋅ v form and on this basis generalize the Zener formula for the inter-band tunneling in homogeneous semiconductors to the case of inter-miniband tunneling in superlattices, account being taken of the inhomogeneity of the electron effective mass. The corresponding sum rule for the effective masses in such structures is obtained. We develop a unified matrix approach for the calculation of the inter-miniband tunneling and Wannier-Stark levels in the case of an arbitrary number of minibands. We study the electric field dependence of the probability of inter-miniband tunneling for an electron transferred through the Brillouin minizone only once. The peculiarities of the inter-miniband transitions for the case where this transfer is repeated are also examined for various unit cells and miniband structures of the superlattice. In addition, we discuss mechanisms and specific features of the resonant Zener tunneling and its manifestations in electron transport.
- Published
- 2011
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25. Relationship between proliferation of Ehrlich ascitic tumor cells and status of the chalone system under conditions of modified photoregimen.
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Mashanova OG, Romanov YA, Antohin AI, Evstaf'ev VV, and Filippovich SS
- Subjects
- Animals, Humans, Carcinoma, Ehrlich Tumor pathology, Cell Proliferation, Light
- Abstract
The effects of permanent darkness on proliferation of Ehrlich ascitic tumor cells and status of the chalone system in the tumor were studied. Chalone-containing preparations from animals exposed to different light conditions exhibited different biological effects on cell proliferation in this tumor. A relationship between biological activity of chalone-containing preparations and sensitivity of tumor cells to these preparations under conditions of modified photoregimen was revealed.
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- 2010
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26. Cell phenotypes in human amniotic fluid.
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Davydova DA, Vorotelyak EA, Smirnova YA, Zinovieva RD, Romanov YA, Kabaeva NV, Terskikh VV, and Vasiliev AV
- Abstract
Stem cells capable of long-term proliferation and differentiation into different cell types may be a promising source of cells for regenerative medicine. Recently, much attention has been paid to fetal stem cells, among which are cells from amniotic fluid (AF). We have isolated amniotic stem cells from 3 AF samples. Flow cytometry, RT -PCR and immunohistochemistry have shown that these cells express mesenchymal (CD90, CD73, CD105, CD13, CD29, CD44, and CD146), neural (≤3-tubulin, Nestin, and Pax6), epithelial (keratin 19 and p63) markers and also markers of pluripotency (Oct4, Nanog, and Rex-1). Transplantation of the cells to nude mice does not lead to tumor formation. Thus, putative stem/progenitor cells from AF are capable of long-term proliferation in vitro and the profile of gene expression led us to speculate that they have greater differentiation potential than mesenchymal stem cells and may be useful for cell therapy.
- Published
- 2009
27. Study of the dynamics of Blastocystis hominis reproduction in vitro.
- Author
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Irikov OA, Antokhin AI, and Romanov YA
- Subjects
- Animals, Culture Media, Blastocystis hominis growth & development
- Abstract
The dynamics of Blastocystis hominis reproduction in vitro in Pavlova's and Nelson-Jones media was studied. The time of generation in these media was 21.5 and 16.7 h, respectively. The duration of the lag phase was 1 day, of log phase 2 days, and of the stationary phase 6 days in both cases. The cell count in the logarithmic phase increased at the expense of the vacuolar forms proliferation. During the stationary phase, the granular forms quantitatively predominated over vacuolar forms, the share of the granular forms reaching almost 100% at late stages of the subculture development.
- Published
- 2009
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28. Activation and damage of endothelial cells upon hypoxia/reoxygenation. Effect of extracellular pH.
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Antonova OA, Loktionova SA, Romanov YA, Shustova ON, Khachikian MV, and Mazurov AV
- Subjects
- Analysis of Variance, Arterial Occlusive Diseases, Caspase 3 metabolism, Cell Adhesion Molecules chemistry, Cells, Cultured, Culture Media, Conditioned, E-Selectin chemistry, E-Selectin metabolism, Endothelium, Vascular cytology, Flow Cytometry, Humans, Hydrogen-Ion Concentration, Intercellular Adhesion Molecule-1 chemistry, Intercellular Adhesion Molecule-1 metabolism, Statistics, Nonparametric, Umbilical Veins, Vascular Cell Adhesion Molecule-1 chemistry, Vascular Cell Adhesion Molecule-1 metabolism, Apoptosis physiology, Cell Adhesion Molecules metabolism, Cell Hypoxia, Endothelial Cells physiology, Oxygen physiology, von Willebrand Factor metabolism
- Abstract
Disturbances of blood flow upon vascular occlusions and spasms result in hypoxia and acidosis, while its subsequent restoration leads to reoxygenation and pH normalization (re-alkalization) in ischemic sites of the vascular bed. The effect of hypoxia/reoxygenation on activation and stimulation of apoptosis in cultured human endothelial cells was studied. The cells were subjected to hypoxia (2% O2, 5% CO2, 93% N(2)) for 24 h followed by reoxygenation (21% O2, 5% CO2, 74% N(2)) for 5 h. Reoxygenation was carried out at different pH-6.4 (preservation of acidosis after hypoxia), 7.0, and 7.4 (partial and complete re-alkalization, respectively). Hypoxia only slightly (by approximately 30%) increased the cell adhesion molecule ICAM-1 content on the cell surface, whereas reoxygenation more than doubled its expression. The reoxygenation effect depended on the medium acidity, and ICAM-1 increase was more pronounced at pH 7.0 compared to that at pH 6.4 and 7.4. Neither hypoxia nor reoxygenation induced expression of two other cell adhesion molecules, VCAM and E-selectin. Incubation of cells under hypoxic conditions but not reoxygenation stimulated secretion of von Willebrand factor and increased its concentration in the culture medium by more than 4 times. The percentage of cells containing apoptosis marker, activated caspase-3, was increased by approximately 1.5 times upon hypoxia as well as hypoxia/reoxygenation. Maximal values were achieved when reoxygenation was performed at pH 7.0. These data show that hypoxia/reoxygenation stimulate pro-inflammatory activation (ICAM-1 expression) and apoptosis (caspase-3 activation) of endothelial cells, and the extracellular pH influences both processes.
- Published
- 2009
- Full Text
- View/download PDF
29. Study of biological rhythms of small intestinal cryptic epithelial mitosis of different periodicity by fourier analysis.
- Author
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Romanov YA, Zharkova NA, Antochin AI, and Zakharchenko AV
- Subjects
- Animals, Fourier Analysis, Intestinal Mucosa, Male, Mice, Epithelial Cells cytology, Intestine, Small cytology, Mitosis, Periodicity
- Abstract
Rhythms of cell division with different periods in the mouse small intestinal cryptic epithelium were studied using Fourier analysis. It was found that the proliferative system of the crypt is characterized by an intricate spatial and temporal organization. The amplitude of low-frequency rhythms increases, while the amplitude of high-frequency rhythms decreased in the direction from the crypt bottom to the neck.
- Published
- 2009
- Full Text
- View/download PDF
30. Specific interaction of cultured human mesenchymal and hemopoietic stem cells under conditions of reduced oxygen content.
- Author
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Zhambalova AP, Darevskaya AN, Kabaeva NV, Romanov YA, and Buravkova LB
- Subjects
- Antigens, Surface metabolism, Cell Hypoxia, Cells, Cultured, Coculture Techniques, Colony-Forming Units Assay, Cytokines metabolism, Hematopoietic Stem Cells cytology, Humans, Mesenchymal Stem Cells cytology, Phenotype, Time Factors, Hematopoietic Stem Cells physiology, Mesenchymal Stem Cells physiology, Oxygen metabolism
- Abstract
We studied the effect of reduced oxygen content (5%) on the phenotype and functional activity of cultured human mesenchymal stem cells. The expression of main immunophenotypic markers for mesenchymal stem cells (CD13, CD29, CD44, CD73, CD90, CD105, and HLA-I) remained practically unchanged under conditions of hypoxia. The expression of cell adhesion molecules (CD54 and CD106) increased during coculturing of mesenchymal stem cells and hemopoietic stem cells. These changes were accompanied by increased production of hemopoietins (interleukin-6 and interleukin-8) and enhanced colony-forming capacity of hemopoietic stem cells. Coculturing of mesenchymal stem cells and hemopoietic stem cells during hypoxia was followed by increased formation of hemopoietic islets and intensive production of interleukin-6, interleukin-8, and vascular endothelial growth factor (compared to cultures under normoxic conditions).
- Published
- 2009
- Full Text
- View/download PDF
31. Damage and activation of endothelial cells during in vitro hypoxia.
- Author
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Antonova OA, Loktionova SA, Golubeva NV, Romanov YA, and Mazurov AV
- Subjects
- Cell Hypoxia, Cells, Cultured, Endothelial Cells cytology, Flow Cytometry, Humans, Intercellular Adhesion Molecule-1 metabolism, Lipopolysaccharides pharmacology, Tumor Necrosis Factor-alpha pharmacology, Umbilical Veins cytology, von Willebrand Factor metabolism, Apoptosis drug effects, Endothelial Cells drug effects, Endothelial Cells metabolism
- Abstract
We studied the effect of hypoxia on activation and stimulation of apoptosis in cultured endothelial cells. The effect of hypoxia was compared to that of apoptosis-inducing agents (tumor necrosis factor and bacterial lipopolysaccharide). Incubation of endothelial cells for 24 h under hypoxic conditions (2% O2, 5% CO2, and 93% N2) increased secretion of von Willebrand factor, but had no effect on the expression of cell adhesion molecule ICAM-1. Tumor necrosis factor and lipopolysaccharide did not stimulate secretion of von Willebrand factor, but significantly increased the expression of ICAM-1. These data attest to significant differences in the mechanisms of endothelium activation under hypoxic conditions and during treatment with tumor necrosis factor or lipopolysaccharide. Hypoxia stimulated apoptosis in endothelial cells, which was seen from the increase in the number of annexin V-binding cells and activation of caspase-3. Similar changes were revealed in the presence of tumor necrosis factor and lipopolysaccharide. Hence, damage to endothelial cells caused by hypoxia and these compounds is mediated by similar mechanisms.
- Published
- 2007
- Full Text
- View/download PDF
32. Vascular endothelium: target or victim of cytostatic therapy?
- Author
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Romanov YA, Chervontseva AM, Savchenko VG, and Smirnov VN
- Subjects
- Cell Adhesion drug effects, Cell Adhesion Molecules metabolism, Cell Line, Cell Proliferation drug effects, Cell Survival drug effects, Endothelial Cells cytology, Endothelial Cells metabolism, Endothelium, Vascular cytology, Endothelium, Vascular drug effects, Endothelium, Vascular metabolism, Humans, Leukocytes cytology, Antineoplastic Agents pharmacology, Cytarabine pharmacology, Daunorubicin pharmacology, Endothelial Cells drug effects
- Abstract
Chemotherapy continues to be the main therapeutic approach in the treatment of hematological malignancies including acute leukemia. Generally, chemotherapy is used to eliminate cancer cells and to restore normal bone marrow function. Simultaneous action of cytostatic drugs on bone marrow angiogenesis decreases the formation of new capillaries and improves therapeutic effect. However, chemotherapeutic agents may also be cytodestructive for cellular elements of other tissues, particularly the vascular endothelium, which can lead to various cardiovascular complications. In this work, we studied the effects of 2 cytostatic drugs, cytosine arabinoside (ara-C) and daunorubicin (DNR), on cultured human vascular (i.e., umbilical) endothelial cells (ECs). Ara-C and DNR were added to cultured cells at concentrations ranging from 1 ng/mL to 100 microg/mL. Drug effects were studied using phase-contrast microscopy, cell viability tests, BRDU incorporation, immunohistochemistry, flow cytometry, and cell cloning. At various concentrations, ara-C and DNR are able to induce morphological and functional changes in cultured cells related to either cytostatic or cytotoxic action. Moreover, ara-C-treated cultured cells displayed significant disturbances in cell adhesion molecule expression and interaction with blood leukocytes. Preliminary data obtained on acute leukemia patients undergoing standard cytostatic therapy ("7+3" regimen) have shown that concentration of the circulating ECs was significantly increased compared with the control group and could be as high as 500-1500 cells/mL of blood. Results obtained suggest that anticancer chemotherapy may induce systemic damage of vascular endothelium related to massive cell loss and (or) alterations of endothelial function.
- Published
- 2007
- Full Text
- View/download PDF
33. Optimum conditions for culturing of human bone marrow and adipose tissue mesenchymal precursor cells.
- Author
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Romanov YA, Darevskaya AN, Kabaeva NV, and Antonova OA
- Subjects
- Biomarkers analysis, Cell Differentiation, Cell Division, Culture Media, Fetal Blood cytology, Humans, Immunohistochemistry, Kinetics, Neurons cytology, Phenotype, Adipose Tissue cytology, Bone Marrow Cells cytology, Cell Culture Techniques methods, Mesenchymal Stem Cells cytology
- Abstract
We present a technology of culturing of human mesenchymal stem cells under conditions excluding the presence of animal sera or additional growth factors, but preserving high proliferative potential and the capacity of these cells to multilineage differentiation. Human umbilical serum was used as the alternative material. We found that in the presence of human umbilical serum mesenchymal stem cells more effectively proliferate and retain their differentiation capacity. The proposed technology yields 109-1010 morphologically and functionally identical cells.
- Published
- 2006
- Full Text
- View/download PDF
34. Oxidized forms of fibrinogen induce expression of cell adhesion molecules by cultured endothelial cells from human blood vessels.
- Author
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Shcheglovitova ON, Azizova OA, Romanov YA, Aseichev AV, Litvina MM, Polosukhina ER, and Mironchenkova EV
- Subjects
- Cells, Cultured, Endothelial Cells metabolism, Endothelium, Vascular cytology, Humans, Oxidation-Reduction, Time Factors, Endothelial Cells drug effects, Fibrinogen pharmacology, Intercellular Adhesion Molecule-1 metabolism, P-Selectin metabolism, Umbilical Veins cytology
- Abstract
Oxidized forms of fibrinogen similarly to initial non-oxidized fibrinogen induced expression of P-selectin and ICAM-1 cell adhesion molecules in the cultured endothelial cells derived from human umbilical vein. The effect of oxidized fibrinogen on the expression of adhesion molecules was more pronounced. These data attest to more active participation of oxidized forms of fibrinogen into inflammation in the vascular wall, the first stage of atherogenesis.
- Published
- 2006
- Full Text
- View/download PDF
35. Effect of chalone system on proliferation of Ehrlich ascitic tumor cells under conditions of modified photoregimen.
- Author
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Mashanova OG, Romanov YA, Evstafyev VV, and Semyonova MV
- Subjects
- Animals, Cell Cycle, Cell Proliferation, Kinetics, Light, Male, Mice, Mitosis, Sodium Chloride pharmacology, Time Factors, Carcinoma, Ehrlich Tumor drug therapy, Carcinoma, Ehrlich Tumor pathology, Chalones pharmacology
- Abstract
We studied the influence of different photoregimens (light/darkness alternation and constant darkness) on the effect of saline and chalone regulation of cell proliferation in Ehrlich ascitic tumor was studied. Saline slightly inhibited proliferation of tumor cells. Chalone-containing preparations from animals exposed to light/darkness alternation and to constant darkness were characterized by different effects on cell proliferation in this tumor. Cell kinetics attested to possible mechanisms of the effect of the chalone system on the status of Ehrlich ascitic tumor; these mechanisms were independent on each other.
- Published
- 2006
- Full Text
- View/download PDF
36. Regularities of spatial and time organization of the proliferative system of small intestinal cryptic epithelium in intact mice in the circahoralian biological rhythm of its cells multiplication.
- Author
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Romanov YA, Zharkova NA, and Filippovich SS
- Subjects
- Animals, Male, Mice, Mice, Inbred Strains, Mitosis, Cell Proliferation, Circadian Rhythm, Intestinal Mucosa physiology, Intestine, Small cytology, Intestine, Small physiology
- Abstract
The proliferative system of the small intestinal cryptic epithelium in intact mice in circahoralian rhythm of mitotic activity of its cells (with a 80-140-min period) is characterized by spatial and time organization. The characteristics of the studied proliferative system do not dub it in individual parts, but present new properties intrinsic of this system in general. The features are greatly similar to the features of spatial and time organization of this proliferative system in circadian rhythm of cell division.
- Published
- 2006
- Full Text
- View/download PDF
37. Circulating bone marrow stem/progenitor cells in vascular atherogenesis and in the noninvasive diagnosis of coronary stenosis.
- Author
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Soboleva EL, Gabbasov ZA, Agapov AA, Akchurin RS, Saburova OS, Romanov YA, and Smirnov VN
- Abstract
Using autopsy specimens and clonal technique, the authors showed that hematopoietic and stromal stem colony-forming units are present in human atheromatous vascular intima. Stromal colony-forming units were also detected in the mononuclear fraction of the blood of patients with hyperlipidemia and coronary stenosis, and were not found in the peripheral blood of normolipidemic volunteers. Using flow cytometry, the absence of stromal circulating colony-forming units in healthy volunteers and their presence in coronary patients was confirmed. It was thought that the presence of circulating stromal precursors with a certain phenotype and variations in their level in blood could serve as an informative noninvasive indicator of coronary stenosis.
- Published
- 2005
38. Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities.
- Author
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Romanov YA, Darevskaya AN, Merzlikina NV, and Buravkova LB
- Subjects
- Biomarkers analysis, Flow Cytometry, Humans, Immunohistochemistry, Mesenchymal Stem Cells physiology, Tubulin, Adipose Tissue cytology, Bone Marrow Cells cytology, Cell Differentiation physiology, Mesenchymal Stem Cells cytology
- Abstract
Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
- Published
- 2005
- Full Text
- View/download PDF
39. Relationship between changes in the photoperiod and proliferation of Ehrlich ascitic tumor cells.
- Author
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Mashanova OG, Romanov YA, Evstafev VV, and Semenova MV
- Subjects
- Animals, Darkness, Homeostasis, Male, Mice, Carcinoma, Ehrlich Tumor, Cell Proliferation, Circadian Rhythm physiology, Photoperiod, Tumor Cells, Cultured
- Abstract
The effect of changed photoperiod (constant darkness) on the proliferation of Ehrlich ascitic tumor cells was demonstrated. Some mechanisms maintaining the proliferative homeostasis in Ehrlich ascitic tumor under conditions of constant darkness were studied, explaining more leveled pattern of circadian rhythms of cell proliferation in animals kept under conditions of constant darkness and indicating the existence of circadian rhythm in sensitivity of Ehrlich ascitic tumor cell to permanent darkness.
- Published
- 2005
- Full Text
- View/download PDF
40. Human chronotope status during short-term visual deprivation.
- Author
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Romanov YA and Irikov OA
- Subjects
- Humans, Biological Clocks, Vision, Ocular
- Abstract
Measuring of an individual minute and individual decimeter by humans with open eyes and eyes closed for 1 min was evaluated. All chronotope parameters were highly individual, but the distribution of time and space measuring values was different. The individual decimeter and, to a lesser extent, chronotope individual decimeter were less in humans subjected to short-term visual deprivation than in subjects with open eyes. Variability of individual minute measuring by humans was less pronounced than the variability of individual decimeter measuring. These differences in variability were less pronounced in the chronotope measured units in subjects with open eyes and were not detected in subjects with short-term visual deprivation.
- Published
- 2005
- Full Text
- View/download PDF
41. The primary effects of clinorotation on cultured human mesenchymal stem cells.
- Author
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Merzlikina NV, Buravkova LB, and Romanov YA
- Subjects
- Actins metabolism, Antigens, CD metabolism, Bone Marrow metabolism, Cells, Cultured, Gravity, Altered, HLA Antigens metabolism, Humans, Cell Proliferation, Gravity Sensing physiology, Mesenchymal Stem Cells physiology, Rotation
- Abstract
Mesenchymal stem cells (MSCs) are specific cells capable of long-term proliferation and differentiation into various stromal tissue cell types. The state of MSCs depends on the cellular microenvironment and several soluble factors. We proposed that gravity could, in addition, influence MSCs features. To prove this hypothesis, we studied the effects of prolonged clinorotation on cultured human MSC morphology, proliferation rate and expression of specific cellular markers. Human bone marrow-derived MSCs were isolated by Histopaque-1.077 density centrifugation and cultured in DMEM-LG with 10% FBS. MSC cultures were composed of fibroblastoid cells negative for hemopoietic cell markers and positive for ASMA, collagen-1, fibronectin, CD54, CD105 and CD106. Cells were exposed to clinorotation from 1 hour to 10 days. It was shown that the proliferative rate was decreased in experimental cultures as compared to cells growing in normal conditions. Clinorotated MSCs appeared more flattened and reached confluence at a lower cell density. The obtained results suggest that cultured human mesenchymal stem cells sense the changes in gravity vector and may respond to microgravity by altered functional activity.
- Published
- 2004
42. Fibrinogen and its oxidized form induce interleukin-8 [correction of interleukin-2] production in cultured endothelial cells of human vessels.
- Author
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Azizova OA, Maksyanina EV, Romanov YA, Aseichev AV, and Scheglovitova ON
- Subjects
- Cells, Cultured, Culture Media, Serum-Free, Endothelial Cells cytology, Endothelium, Vascular metabolism, Humans, Oxidation-Reduction, Endothelial Cells metabolism, Endothelium, Vascular cytology, Fibrinogen metabolism, Interleukin-8 metabolism
- Abstract
Oxidized fibrinogen was more potent than native fibrinogen in inducing interleukin-8 production in primary culture of human endothelial cells. The optimal concentration of oxidized fibrinogen was 3 mg/ml. The optimal time of UV irradiation was 17 min. Secretion of interleukin-8 was maximum during culturing of endothelial cells in a serum-free medium.
- Published
- 2004
- Full Text
- View/download PDF
43. Spatiotemporal organization of the proliferative system in small intestinal crypt epithelium of intact mice.
- Author
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Romanov YA, Antokhin AI, Kozlova AY, Zharkova NA, Malysheva IN, and Filippovich SS
- Subjects
- Animals, Animals, Outbred Strains, Circadian Rhythm, Male, Mice, Mitotic Index, Photoperiod, Cell Division physiology, Epithelial Cells cytology, Epithelial Cells physiology, Intestinal Mucosa cytology, Intestine, Small cytology, Microvilli physiology
- Abstract
We studied spatiotemporal organization of the proliferative system in small intestinal crypt epithelium of normal mice. Close relationships were found between circadian rhythms of cell proliferation and their position in the crypt. These peculiarities reflected spatiotemporal organization of the crypt epithelium. The hierarchic structure of spatiotemporal organization suggests the existence of several interrelated levels (individual cells, cell subpopulations, and cells with basal and maximum levels of proliferation within subpopulation). Each level has its proper temporal and spatial characteristics. Their interaction determines spatiotemporal organization of the proliferative system in the small intestinal crypt epithelium.
- Published
- 2003
- Full Text
- View/download PDF
44. Spatiotemporal characteristics of glycogen content in rat liver lobule.
- Author
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Markina VV and Romanov YA
- Subjects
- Animals, Hepatocytes cytology, Hepatocytes metabolism, Liver cytology, Liver Glycogen metabolism, Male, Photometry methods, Rats, Circadian Rhythm physiology, Liver metabolism, Liver Glycogen analysis
- Abstract
We evaluated spatiotemporal characteristics of glycogen content in cells of rat liver lobule. Glycogen content in the liver lobule and its circulatory subzones underwent diurnal fluctuations with acrophase at 5.00. Spatial changes were characterized by a dependence of gradients in the lobule on the phase of diurnal rhythmic fluctuations. Total glycogen content in the peripheral subzone of the lobule 5.2-fold surpassed that in the central subzone. Our results illustrate spatiotemporal organization of glycogen content in the liver lobule.
- Published
- 2003
- Full Text
- View/download PDF
45. Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord.
- Author
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Romanov YA, Svintsitskaya VA, and Smirnov VN
- Subjects
- Endothelium, Vascular cytology, Gestational Age, Humans, Infant, Newborn, Umbilical Veins cytology, Mesoderm cytology, Stem Cells chemistry, Umbilical Cord cytology
- Abstract
Mesenchymal stem cells (MSCs) have the capability for renewal and differentiation into various lineages of mesenchymal tissues. These features of MSCs attract a lot of attention from investigators in the context of cell-based therapies of several human diseases. Despite the fact that bone marrow represents the main available source of MSCs, the use of bone marrow-derived cells is not always acceptable due to the high degree of viral infection and the significant drop in cell number and proliferative/differentiation capacity with age. Thus, the search for possible alternative MSC sources remains to be validated. Umbilical cord blood is a rich source of hematopoietic stem/progenitor cells and does not contain mesenchymal progenitors. However, MSCs circulate in the blood of preterm fetuses and may be successfully isolated and expanded. Where these cells home at the end of gestation is not clear. In this investigation, we have made an attempt to isolate MSCs from the subendothelial layer of umbilical cord vein using two standard methodological approaches: the routine isolation of human umbilical vein endothelial cell protocol and culture of isolated cells under conditions appropriate for bone-marrow-derived MSCs. Our results suggest that cord vasculature contains a high number of MSC-like elements forming colonies of fibroblastoid cells that may be successfully expanded in culture. These MSC-like cells contain no endothelium- or leukocyte-specific antigens but express alpha-smooth muscle actin and several mesenchymal cell markers. Therefore, umbilical cord/placenta stroma could be regarded as an alternative source of MSCs for experimental and clinical needs.
- Published
- 2003
- Full Text
- View/download PDF
46. Measurements of time and space as manifestation of spatiotemporal organization of reflection.
- Author
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Romanov YA and Irikov OA
- Subjects
- Adolescent, Exercise, Hearing, Heart Rate, Humans, Individuality, Male, Time Factors, Perception physiology, Space Perception, Time Perception
- Abstract
We studied measurements of the individual minute and individual decimeter. Individual time and length measures were reproduced separately or in combination (drawing 1 dm over 1 min). The measurements of time and space as coordinates of a vector for the space-and-time image are a single system of spatiotemporal organization of reflection. We revealed individual differences in the measurement of time and space. The individual minute was more variable than the individual decimeter.
- Published
- 2002
- Full Text
- View/download PDF
47. Herpes simplex type I virus infected human vascular endothelial cells induce the production of anti-viral and proinflammatory factors by peripheral blood leukocytes in vitro.
- Author
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Scheglovitova ON, Romanov YA, Maksianina EV, Svintsitskaya VA, and Pronin AG
- Subjects
- Antibodies immunology, Cell Adhesion Molecules metabolism, Cells, Cultured, Culture Media, Conditioned metabolism, Endothelium, Vascular immunology, Endothelium, Vascular pathology, Humans, Interferons immunology, Tumor Necrosis Factor-alpha immunology, Cell Communication immunology, Endothelium, Vascular metabolism, Endothelium, Vascular virology, Herpesvirus 1, Human metabolism, Leukocytes immunology
- Abstract
Viral infection of the vascular wall cellular elements is involved in development of several pathophysiological events, including vasculitis, transplant rejection, and atherosclerosis. Previously, we have shown that cultured human vascular endothelial cells (ECs) may be effectively infected with herpes simplex type I virus (HSV-1), and this cultural model could be a useful tool for the explanation of many aspects of viral disease. In this study, we investigated the effects of conditioned media (CM) of peripheral blood mononuclear cells (PBMCs) on HSV-1 reproduction and cell adhesion molecule expression in cultured ECs. PBMC-CM induced the delay of virus reproduction or inhibition of virus reproduction. Effects of CM correlated with multiplicity of infection used for EC, time of PBMC contact with infected and glutaraldehyde-fixed endothelium, and the level of IFNs and cytokine production. Passages of and CM-treated and infected cells without signs of virus reproduction were, sometimes, followed by virus reactivation. However, at a low level of infection of CM-treated ECs the virus reactivation was not observed even after 2-3 cell passages. Neutralizing antibodies against IFN-alpha, IFN-gamma, and TNF-alpha, used separately or together, significantly abrogated the delaying and/or inhibiting action of CM. Additionally, PBMC-CM significantly increased the expression of ICAM-1 and VCAM-1 on cultured ECs. The strongest cell activation was induced by CM obtained from PBMCs co-incubated with virus-infected endothelium. Obtained results suggest that primed leukocytes produce soluble factors with either anti-viral or pro-inflammatory activity, and the effect of PBMC-CM may have a bi-directional action. On the one hand, due to production of interferons and several cytokines CM sets up HSV-1 latency or virus elimination from cultured cells. On the other, the same cytokines act on infected and/or neighboring ECs and initiate the cascade of inflammatory reactions in the vascular wall.
- Published
- 2002
48. Ultradian biorhythms of cell proliferation.
- Author
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Romanov YA, Evstaf'ev VV, Rybakov VP, and Irikov OA
- Subjects
- Animals, Epithelial Cells cytology, Esophagus cytology, Intestine, Small cytology, Intestines cytology, Light, Male, Mice, Mitosis, Thymus Gland cytology, Tongue cytology, Cell Division, Circadian Rhythm
- Abstract
We studied circadian and ultradian rhythms of mitotic index of epithelial cells of the esophagus, intestine, and dorsal surface of the tongue, and thymocytes from mice maintained at normal and inverted light regimens. It was shown that in normal animals, the period of ultradian oscillations was shorter during the active circadian phase compared to the passive phase. Photoinversion changed the correlation between ultradian oscillations of the mitotic index and its circadian phases, which was differently pronounced in various tissues. Thus, changes in daily illumination serve as the time indicator for circadian rhythms of cell proliferation, but not for ultradian oscillations of mitotic activity.
- Published
- 2002
- Full Text
- View/download PDF
49. Chronobiological regularities of protein content in rat retinal ganglion cells during exposure to products of Astrakhan gas-processing plant.
- Author
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Nevalennaya LA, Romanov YA, and Bekchanov AN
- Subjects
- Animals, Embryo, Mammalian, Environmental Pollution, Female, Periodicity, Pregnancy, Rats, Retinal Ganglion Cells metabolism, Russia, Fossil Fuels, Hydrogen Sulfide adverse effects, Maternal-Fetal Exchange, Pregnancy, Animal, Proteins metabolism, Retinal Ganglion Cells drug effects
- Abstract
Biorhythms of protein content in rat retinal ganglion cells during pregnancy are sensitive to exposure to natural gas of the Astrakhan gas condensate plant. Diurnal rhythm of sensitivity to hydrogen sulfide was revealed.
- Published
- 2002
- Full Text
- View/download PDF
50. Structure of vascular plexuses of brain ventricles in ontogeny under normal conditions and in hypoxia.
- Author
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Tkacheva NV, Romanov YA, Sentyurova LG, and Belopasov VV
- Subjects
- Glycosaminoglycans metabolism, Humans, Hypoxia pathology, Infant, Newborn, Iron metabolism, Succinate Dehydrogenase metabolism, Cerebral Ventricles blood supply, Hypoxia metabolism
- Abstract
The structure of vascular plexuses of brain ventricles in newborns developed under hypoxic conditions does not correspond to gestational age. Chronic hypoxia decreases activity of succinate dehydrogenase and iron content in vascular plexuses of brain ventricles.
- Published
- 2002
- Full Text
- View/download PDF
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