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1. The breast cancer tumor microenvironment and precision medicine: immunogenicity and conditions favoring response to immunotherapy

Author: Andrea Nicolini, Paola Ferrari, Roberto Silvestri, and Federica Gemignani
Subjects: Breast cancer, Tumor microenvironment, Immunotherapy, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract: Some main recent researches that have dissected tumor microenvironment (TME) by imaging mass cytometry (IMC) in different subtypes of primary breast cancer samples were considered. The many phenotypic variants, clusters of epithelial tumor and immune cells, their structural features as well as the main genetic aberrations, sub-clonal heterogeneity and their systematic classification also have been examined. Mutational evolution has been assessed in primary and metastatic breast cancer samples. Overall, based on these findings the current concept of precision medicine is questioned and challenged by alternative therapeutic strategies. In the last two decades, immunotherapy as a powerful and harmless tool to fight cancer has received huge attention. Thus, the tumor immune microenvironment (TIME) composition, its prognostic role for clinical course as well as a novel definition of immunogenicity in breast cancer are proposed. Investigational clinical trials carried out by us and other findings suggest that G0-G1 state induced in endocrine-dependent metastatic breast cancer is more suitable for successful immune manipulation. Residual micro-metastatic disease seems to be another specific condition that can significantly favor the immune response in breast and other solid tumors.
Published: 2024
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2. Plasma Brain-Derived Neurotrophic Factor (BDNF) Levels and BDNF Promoters’ DNA Methylation in Workers Exposed to Occupational Stress and Suffering from Psychiatric Disorders

Author: Antonello Veltri, Vanessa Nicolì, Riccardo Marino, Filomena Rea, Martina Corsi, Martina Chiumiento, Marianna Giangreco, Fabrizio Caldi, Giovanni Guglielmi, Rudy Foddis, Fabio Coppedè, Roberto Silvestri, and Rodolfo Buselli
Subjects: brain-derived neurotrophic factor, BDNF exon I and IV promoters’ methylation, epigenetic changes, work-related stress, adjustment disorders, major depressive disorder, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract: Introduction: Decreased plasma BDNF (pBDNF) levels have been proposed as a biomarker in the illness phases of mood disorders. This cross-sectional study aimed to evaluate the pBDNF and BDNF promoters’ DNA methylation levels in workers exposed to occupational stress and suffering from work-related stress disorders. Methods: the pBDNF and BDNF exon I and IV promoters’ methylation levels were measured by specific immunoassays and methylation-sensitive high-resolution melting (MS-HRM) in 62 patients with adjustment disorders (AD), 79 patients with major depressive disorder (MDD) and 44 healthy controls. Occupational stress was evaluated in the patients and controls using the Job Content Questionnaire (JCQ). Results: the pBDNF levels were significantly higher in the MDD (p < 0.001) and AD (p < 0.0001) patients than in the controls. The MDD patients showed significantly lower pBDNF levels than the AD ones (p = 0.01). The BDNF exon I and IV promoters’ methylation levels were significantly higher in the MDD patients than in the AD ones (exon I promoter: p = 0.0001, exon IV promoter: p < 0.0001) and controls (exon I promoter: p = 0.0001, exon IV promoter: p < 0.0001). In the patients, but not in the controls, the BDNF promoters’ methylation levels showed significant negative correlations with occupational stress. Conclusions: BDNF could play a key role in the pathophysiology of stress-related disorders and the peripheral elevation of it observed in patients exposed to occupational stress could suggest a protective mechanism for neurons from stress-mediated damage. The elevation of the pBDNF levels, even in MDD, may characterize a “reactive” subtype of depressive episode, while the significant elevation of the BDNF promoters’ methylation levels in depressed patients could indicate a predisposition to more severe illness under stress. Further research is needed, focusing on biomarkers for stress-related disorders as a potential tool for the diagnosis and prevention of occupational diseases.
Published: 2024
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3. Comparative analysis of genetic variants in pleural fluids and solid tissue biopsies of pleural mesothelioma patients: Implications for molecular heterogeneity assessment

Author: Roberto Silvestri, Filomena Rea, Marianna Vitiello, Gabriele Moretti, Vittorio Aprile, Marco Lucchi, Paolo Aretini, Chiara Maria Mazzanti, Stefano Landi, and Federica Gemignani
Subjects: Liquid biopsies, Pleural mesothelioma, Cancer-specific mutations, Whole-exome sequencing, Tumour heterogeneity, Science (General), Q1-390, Social sciences (General), H1-99
Abstract: Objectives: This study aims to determine whether the sequencing of DNA extracted from pleural fluids (PFs) of Pleural Mesothelioma (PM) patients accurately represents the genetic information obtained from the solid tissue counterpart biopsies with particular attention to the identification of single nucleotide variants (SNVs). Materials and methods: Single pleural biopsy, PFs, and blood were collected from PM patients. DNA was extracted from these samples and then subjected to Whole-Exome Sequencing. Results: A higher number of SNVs was identified in PFs than in solid tissue biopsies (STBs). Most SNVs were detected in PFs samples but not in STBs samples, while only a few SNVs were detected in STBs samples but not in PFs samples. Conclusion: The current findings support the notion that PFs might offer a more robust depiction of cancer's molecular diversity. Nonetheless, the current outcomes challenge the assertion that liquid biopsies can encompass the entirety of intra-patient variations. Indeed, a subset of potential cancer-driver SNVs was exclusively identified in STBs. However, relying solely on STBs would have precluded the detection of significant SNVs that were exclusively present in PFs. This implies that while PFs serve as a valuable complement to STBs, they do not supplant them.
Published: 2024
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4. Polymorphism Pro64His within galectin-3 has functional consequences at proteome level in thyroid cells

Author: Roberto Silvestri, Lorenzo Zallocco, Alda Corrado, Maurizio Ronci, Romina Aceto, Benedetta Ricci, Monica Cipollini, Irene Dell’Anno, Chiara De Simone, Giuseppina De Marco, Eleonora Ferrarini, Daniela Beghelli, Maria Rosa Mazzoni, Antonio Lucacchini, Federica Gemignani, Laura Giusti, and Stefano Landi
Subjects: galectin-3 (gal3), functional polymorphism rs4644, differentiated thyroid carcinoma (DTC), CRISPR/Cas9, proteome, papillary thyroid carcinoma (PTC), Genetics, QH426-470
Abstract: IntroductionThe single nucleotide polymorphism (SNP) rs4644 at codon 64 of galectin-3 (gal-3, gene name: LGALS3), specifying the variant proline (P64) to histidine (H64), is known to affect the protein’s functions and has been associated with the risk of several types of cancer, including differentiated thyroid carcinoma (DTC).Materials and methodsTo deepen our understanding of the biological effects of this SNP, we analyzed the proteome of two isogenic cell lines (NC-P64 vs. NA-H64) derived from the immortalized non-malignant thyrocyte cell line Nthy-Ori, generated through the CRISPR-Cas9 technique to differ by rs4644 genotype. We compared the proteome of these cells to detect differentially expressed proteins and studied their proteome in relation to their transcriptome.ResultsFirstly, we found, consistently with previous studies, that gal-3-H64 could be detected as a monomer, homodimer, and heterodimer composed of one cleaved and one uncleaved monomer, whereas gal-3-P64 could be found only as a monomer or uncleaved homodimer. Moreover, results indicate that rs4644 influences the expression of several proteins, predominantly upregulated in NA-H64 cells. Overall, the differential protein expression could be attributed to the altered mRNA expression, suggesting that rs4644 shapes the function of gal-3 as a transcriptional co-regulator. However, this SNP also appeared to affect post-transcriptional regulatory mechanisms for proteins whose expression was oppositely regulated compared to mRNA expression. It is conceivable that the rs4644-dependent activities of gal-3 could be ascribed to the different modalities of self-dimerization.ConclusionOur study provided further evidence that rs4644 could affect the gal-3 functions through several routes, which could be at the base of differential susceptibility to diseases, as reported in case-control association studies.
Published: 2024
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5. Genetic signature of differentiated thyroid carcinoma susceptibility: a machine learning approach

Author: Giulia Brigante, Clara Lazzaretti, Elia Paradiso, Federico Nuzzo, Martina Sitti, Frank Tüttelmann, Gabriele Moretti, Roberto Silvestri, Federica Gemignani, Asta Försti, Kari Hemminki, Rossella Elisei, Cristina Romei, Eric Adriano Zizzi, Marco Agostino Deriu, Manuela Simoni, Stefano Landi, and Livio Casarini
Subjects: differentiated thyroid cancer, machine learning, single nucleotide polymorphism, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract: To identify a peculiar genetic combination predisposing to diffe rentiated thyroid carcinoma (DTC), we selected a set of single nucleotide polymorphisms (SNPs) associated with DTC risk, considering polygenic risk score (PRS), Bayesian statistics and a machine learning (ML) classifier to describe cases and controls in three different datasets. Dataset 1 (649 DTC, 431 controls) has been previously genotyped in a genome-wide association study (GWAS) on Italian DTC. Dataset 2 (234 DTC, 101 controls) and dataset 3 (404 DTC, 392 controls) were genotyped. Associations of 171 SNPs reported to predispose to DTC in candidate studies were extracted from the GWAS of dataset 1, followed by replication of SNPs associated with DTC risk (P < 0.05) in dataset 2. The reliability of the identified SNPs was confirmed by PRS and Bayesian statistics after merging the three datasets. SNPs were used to describe the case/control state of individual s by ML classifier. Starting from 171 SNPs associated with DTC, 15 were positive in both datasets 1 and 2. Using these markers, PRS revealed that individuals in the fif th quintile had a seven-fold increased risk of DTC than those in the first. Bayesian inf erence confirmed that the selected 15 SNPs differentiate cases from controls. Results were corroborated by ML, finding a maximum AUC of about 0.7. A restricted selection of on ly 15 DTC-associated SNPs is able to describe the inner genetic structure of Italian individuals, and ML allows a fair prediction of case or control status based solely on the individual genetic background.
Published: 2023
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6. Tissue expression of lactate transporters (MCT1 and MCT4) and prognosis of malignant pleural mesothelioma (brief report)

Author: Irene Dell’Anno, Elisa Barone, Luciano Mutti, Doris M. Rassl, Stefan J. Marciniak, Roberto Silvestri, Stefano Landi, and Federica Gemignani
Subjects: Malignant pleural mesothelioma (MPM), MCT1, MCT4, Basigin (CD147), Immunohistochemistry (IHC), Tissue microarray (TMA), Medicine
Abstract: Abstract Background Malignant pleural mesothelioma (MPM) is an aggressive neoplasm of the pleura, mainly related to asbestos exposure. As in other solid tumors, malignant cells exhibit high glucose uptake and glycolytic rates with increased lactic acid efflux into the interstitial space. Lactate transport into and out of cells, crucial to maintaining intracellular pH homeostasis and glycolysis, is carried out by monocarboxylate transporters (MCTs) and the chaperone basigin (CD147). We set out to examine the clinical significance of basigin, MCT1 and MCT4 in the context of MPM and to evaluate their expression in relation to the evolution of the disease. Methods We used immunohistochemistry to measure the expression of basigin, MCT1 and MCT4 in a cohort of 135 individuals with MPM compared to a series of 15 non-MPM pleura specimens. Moreover, by Kaplan–Meier and Cox analyses we evaluated whether an expression over the average of these markers could be associated with the patients’ overall survival (OS). Results We detected positive staining of basigin, MCT1, and MCT4 in most MPM specimens. In particular, MCT4 was always positive in malignant tissues but undetectable in the 4 normal pleural specimens incorporated within the tissue microarray. This was confirmed in the additional series of 15 normal pleural samples. Moreover, MCT4 expression was significantly associated with reduced OS. Conclusion In this study, the tissue expression of basigin did not prove to be exploitable as a diagnostic or prognostic marker for MPM patients. The expression of MCT1 was not informative either, being tightly correlated with that of basigin. However, the expression of MCT4 showed promise as a diagnostic/therapeutic and prognostic biomarker.
Published: 2020
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7. Role of Prosaposin and Extracellular Sulfatase Sulf-1 Detection in Pleural Effusions as Diagnostic Biomarkers of Malignant Mesothelioma

Author: Lorenzo Zallocco, Roberto Silvestri, Federica Ciregia, Alessandra Bonotti, Riccardo Marino, Rudy Foddis, Antonio Lucacchini, Laura Giusti, and Maria Rosa Mazzoni
Subjects: mesothelioma, pleural effusion, lung adenocarcinoma, biomarker, prosaposin, extracellular sulfatase Sulf-1, Biology (General), QH301-705.5
Abstract: Malignant pleural mesothelioma is an aggressive malignancy with poor prognosis. Unilateral pleural effusion is frequently the initial clinical sign requiring therapeutic thoracentesis, which also offers a diagnostic opportunity. Detection of soluble biomarkers can support diagnosis, but few show good diagnostic accuracy. Here, we studied the expression levels and discriminative power of two putative biomarkers, prosaposin and extracellular sulfatase SULF-1, identified by proteomic and transcriptomic analysis, respectively. Pleural effusions from a total of 44 patients (23 with mesothelioma, 8 with lung cancer, and 13 with non-malignant disease) were analyzed for prosaposin and SULF-1 by enzyme-linked immunosorbent assay. Pleural effusions from mesothelioma patients had significantly higher levels of prosaposin and SULF-1 than those from non-malignant disease patients. Receiver-operating characteristic (ROC) analysis showed that both biomarkers have good discriminating power as pointed out by an AUC value of 0.853 (p = 0.0005) and 0.898 (p < 0.0001) for prosaposin and SULF-1, respectively. Combining data ensued a model predicting improvement of the diagnostic performance (AUC = 0.916, p < 0.0001). In contrast, prosaposin couldn’t discriminate mesothelioma patients from lung cancer patients while ROC analysis of SULF-1 data produced an AUC value of 0.821 (p = 0.0077) but with low sensitivity. In conclusion, prosaposin and SULF-1 levels determined in pleural effusion may be promising biomarkers for differential diagnosis between mesothelioma and non-malignant pleural disease. Instead, more patients need to be enrolled before granting the possible usefulness of these soluble proteins in differentiating mesothelioma pleural effusions from those linked to lung cancer.
Published: 2022
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8. Evaluation of the Anti-Spike (RDB) IgG Titer among Workers Employed at the University of Pisa Vaccinated with Different Types of SARS-CoV-2 Vaccines

Author: Rudy Foddis, Riccardo Marino, Roberto Silvestri, Poupak Fallahi, Salvio Perretta, Christian Garaffa, Riccardo Morganti, Martina Corsi, Jonathan Mennucci, Francesco Porciatti, Gianluca Nerli, Rodolfo Buselli, Antonello Veltri, Fabrizio Caldi, Giovanni Guglielmi, Grazia Luchini, Silvia Briani, Donatella Talini, and Francesco Cipriani
Subjects: SARS-CoV-2 infection, COVID-19, vaccines, healthcare workers, occupational medicine, Medicine
Abstract: With the development of SARS-CoV-2 vaccines, many authors started evaluating the immunization efficacy of the available vaccines mainly through sero-positivity tests or by a quantitative assessment of the IgG against the spike protein of SARS-CoV-2 virus in vaccinated subjects. In this work, we compared the titers resulting from vaccination and tried to understand the potential factors affecting the immune response to the available SARS-CoV-2 vaccines. This study was conducted on 670 volunteers employed at the University of Pisa and undergoing a health surveillance program at the University Hospital of Pisa. For each participant, 10 mL of blood, information about contacts with confirmed cases of COVID-19, age, sex, SARS-CoV-2 vaccination status, previous SARS-CoV-2 infection and symptoms, type of vaccine and the date of administration were collected. In the multivariate analysis, the type of vaccine, the presence of symptoms in SARS-CoV-2 positive individuals, and the distance from the second dose significantly affected the antibody titer; the combined vaccination resulted in a faster decay over time compared with the other types of vaccination. No significant differences were observed between Spikevax and Comirnaty (p > 0.05), while the antibody levels remain more stable in subjects undergoing Vaxzevria vaccination (p < 0.01) compared with mRNA-based ones.
Published: 2022
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9. Variation rs2235503 C > A Within the Promoter of MSLN Affects Transcriptional Rate of Mesothelin and Plasmatic Levels of the Soluble Mesothelin-Related Peptide

Author: Roberto Silvestri, Perla Pucci, Chiara De Santi, Irene Dell’Anno, Simona Miglietta, Alda Corrado, Vanessa Nicolí, Daniela Marolda, Monica Cipollini, Enrica Pellegrino, Monica Evangelista, Alessandra Bonotti, Rudy Foddis, Alfonso Cristaudo, Stefano Landi, and Federica Gemignani
Subjects: soluble mesothelin-related peptide, malignant pleural mesothelioma, diagnostic biomarkers, single nucleotide polymorphisms, fluorescent reporter assay, eQTL, Genetics, QH426-470
Abstract: Soluble mesothelin-related peptide (SMRP) is a promising biomarker for malignant pleural mesothelioma (MPM), but several confounding factors can reduce SMRP-based test’s accuracy. The identification of these confounders could improve the diagnostic performance of SMRP. In this study, we evaluated the sequence of 1,000 base pairs encompassing the minimal promoter region of the MSLN gene to identify expression quantitative trait loci (eQTL) that can affect SMRP. We assessed the association between four MSLN promoter variants and SMRP levels in a cohort of 72 MPM and 677 non-MPM subjects, and we carried out in vitro assays to investigate their functional role. Our results show that rs2235503 is an eQTL for MSLN associated with increased levels of SMRP in non-MPM subjects. Furthermore, we show that this polymorphic site affects the accuracy of SMRP, highlighting the importance of evaluating the individual’s genetic background and giving novel insights to refine SMRP specificity as a diagnostic biomarker.
Published: 2020
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10. Big and Free Fractions of Gamma-Glutamyltransferase: New Diagnostic Biomarkers for Malignant Mesothelioma?

Author: Rudy Foddis, Maria Franzini, Alessandra Bonotti, Riccardo Marino, Roberto Silvestri, Poupak Fallahi, Dante Chiappino, Michele Emdin, Aldo Paolicchi, and Alfonso Cristaudo
Subjects: mesothelioma, biomarkers, diagnosis, gamma-glutamyltransferase, Medicine (General), R5-920
Abstract: Malignant pleural mesothelioma (MPM) is a cancer mainly caused by asbestos fiber inhalation, characterized by an extremely long latency and poor prognosis. Recently, researchers have focused on testing the diagnostic ability of several biomarkers. Gamma-Glutamyltransferase (GGT) has been demonstrated to be the sum of several GGT sub-fractions activity, classified based on their molecular weight in big-GGT, medium-GGT, small-GGT, and free-GGT. This work aims to evaluate whether specific GGT fractional enzymatic activity patterns could be helpful in MPM diagnosis. We analyzed blood samples from 175 workers previously exposed to asbestos, 157 non-exposed healthy subjects, and 37 MPM patients through a molecular exclusion chromatographic method. We found a specific profile of GGT fractions activity, significantly associated with MPM, resulting in an increase in b-, m- activity, along with an evident, yet not significant, decrease in f-activity. Receiver-operating characteristic (ROC) analysis showed that the best Area Under Curve (AUC) value resulted from the combined index b/f (0.679, 95% CI: 0.582–0.777). Combining the b-/f-GGT activity with the levels of serum mesothelin-related protein (SMRP; another promising MPM biomarker) improved the diagnostic accuracy, increasing the AUC value to 0.875 (95% CI: 0.807–0.943, p =
Published: 2022
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11. Understanding Factors Associated With Psychomotor Subtypes of Delirium in Older Inpatients With Dementia

Author: Rispoli, Vincenzo, Malara, Alba, Spadea, Fausto, Di Cello, Serena, Ceravolo, Francesco, Fabiano, Francesco, Chiaradia, Giuseppe, Gabriele, Amedeo, Lenino, Peluso, Andrea, Taristano, Settembrini, Vincenzo, Capomolla, Domenico, Citrino, Antonella, Scriva, Antonietta, Bruno, Irene, Secchi, Roberto, De Martino, Eugenio, Muccinelli, Roberto, Lupi, Gerardo, Paonessa, Patrizio, Fabbri, Andrea, Passuti, Maria Teresa, Castellari, Sofia, Po, Andrea, Gaggioli, Guido, Varesi, Massimo, Moneti, Paolo, Capurso, Sebastiano, Latini, Vincenzo, Ghidotti, Stefano, Riccardelli, Francesco, Macchi, Maurizio, Rigo, Rosaria, Claudio, Pascale, Angelo, Bosio, Flavio, Cerrato, Benedetta, Bardelli, Boffelli, Stefano, Cassinadri, Angela, Franzoni, Simone, Spazzini, Elena, Andretto, Daniela, Tonini, Gabriele, Andreani, Laura, Coralli, Mirco, Balotta, Antonio, Cancelliere, Roberto, Ballardini, Giorgio, Simoncelli, Margherita, Mancini, Annarita, Strazzacapa, Mara, Fabio, Stefano, De Filippi, Francesco, Giudice, Chiara, Dentizzi, Cosimo, Azzini, Margherita, Cazzadori, Marco, Mastroeni, Valeria, Bertassello, Paolo, Claudia Benati, Helena Santana, Nesta, Elisa, Tobaldini, Chiara, Guerini, Fabio, Elena, Tambara, Mombelloni, Paolo, Fontanini, Fulvio, Gabriella, Lassa, Pizzorni, Crosio, Oliverio, Martina, Del Grosso, Luciano Luca, Giavedoni, Cristina, Bidoli, Giuliano, Mazzei, Bruno, Corsonello, Andrea, Fusco, Sergio, Vena, Silvio, De Vuono, Tommaso, Maiuri, Giorgio, Luca, Fimognari Filippo, Andrea, Arone, Giovanni, Sgrò, Rossella, Nicolazzo, Castegnaro, Eugenio, De Rosa, Salvatore, Sechi, Rossella Bazzano, Benvenuti, Enrico, Del Lungo, Ilaria, Giardini, Sante, Giulietti, Chiara, Mauro, Di Bari, Eleonora, Barghini, Martina, Paoli, Irene, Fiordelli, Riccardo, Barucci, Federica, Sgrilli, Ilaria, Del Lungo, Bertoletti, Erik, D'Amico, Ferdinando, Caronzolo, Francesco, Grippa, Alessandro, Lombardo, Giuseppina, Pipicella, Tiziana, Antonino, Saitta, Francesco, Corica, Valeria, Prestipino Giarritta, Daniela, Larosa, Domenico, Cucinotta, Giorgio, Basile, March, Albert, Nitti, Maria Teresa, Felici, Alessandro, Pavan, Silvia, Piazzani, Fabrizio, Lunelli, Alessandra, Dimori, Sergio, Margotta, Alessandro, Soglia, Tiziano, Postacchini, Demetrio, Brunelli, Roberto, Santini, Silvia, Francavilla, Monia, Macchiati, Ilenia, Sorvillo, Francesca, Giuli, Cinzia, Mecocci, Patrizia, Longo, Annalisa, Perticone, Francesco, Addesi, Desireè, Rosa, Paola Cerra, Bencardino, Giuseppe, Falbo, Tania, Grillo, Nadia, Marco, Filice, Mirella, Flippo, Fantò, Fausto, Isaia, Gianluca, Pezzilli, Stella, Bergamo, Daniele, Furno, Elisabetta, Rrodhe, Sokol, Lucarini, Simonetta, Dijk, Babette, Dall'Acqua, Francesca, Cappelletto, Francesco, Calvani, Donatella, Becheri, Dimitri, Giuseppe, Mottino, Costanza, Mitidieri, Vito, Antonio, Francesca, Bartalucci, Magherini, Lorenza, Novella, Malin, Franca, Boni, Lucia Gambardella, Paoli Martina, Valente, Carlo, Ilaria, Bracali, Alice, Foschini, Bo, Mario, Porrino, Paola, Ceci, Giacomo, Giuliana, Bottignole, Michela, Tibaldi, Eleonora, Coppo, Ettore, Evaristo, Camellini, Cinzia, Servello, Adriana, Grassi, Alessandro, Rozzini, Renzo, Tironi, Sara, Grassi, Maria Grazia, Troisi, Elio, Carlo, Caltagirone, Simona Gabriella, Di Santo, Flaminia, Franchini, Federica, Ratto, Beatrice, Pavoni, Sofia, Toniolo, Gabutto, Anna, Quazzo, Loredana, Rosatello, Annalisa, Suraci, Domenico, Tagliabue, Benedetta, Perrone, Chiara, Ferrara, Lucia, Castagna, Alberto, Tremolada, Maria Luisa, Giuseppe, Castoldi, Stefano, Barbero, Davide, Oltramonti, Piano, Simonetta, Serviddio, Gaetano, Lo Buglio, Aurelio, Gurrera, Tiziana, Merlo, Valeria, Rovai, Carla, Cotroneo, Antonino Maria, Carlucci, Rosaria, Abbaldo, Anna, Monzani, Fabio, Qasem, Ahmad Amedeo, Bini, Giacomo, Tafuto, Silvia, Galli, Giovanni, Bruni, Amalia Cecilia, Mancuso, Giovanna, Mancuso, Gerardo, Calipari, Daniela, Giuseppe Massimiliano, De Luca, Bernardini, Bruno, Corsini, Carla, Michele, Ciccarelli, Sara, Dal Farra, Cagnin, Annachiara, Fragiacomo, Federica, Pompanin, Sara, Piero, Amodio, Marco, Cagnin, Zurlo, Amedeo, Guerra, Gianluca, Pala, Marco, Menozzi, Luca, Gatti, Chiara Delli, Magon, Stefania, Roberto, Manfredini, Alfredo, De Giorgi, Fabio, Fabbian, Ruana, Tiseo, Elisa, Misurati, Benedetta, Boari, Christian, Molino, Marco, Pala, Massimo, Gallerani, Di Francesco, Vincenzo, Faccioli, Silvia, Pellizzari, Luca, Giorgia, Fontana, Barbagallo, Giuseppe, Lunardelli, Maria Lia, Martini, Emilio, Ferrari, Eleonora, Macchiarulo, Maria, Corneli, Maria, Bacci, Monica, Battaglia, Giuseppe, Anastasio, Luigi, Lo Storto, Mario Sostene, Seresin, Chiara, Simonato, Matteo, Loreggian, Michele, Cestonaro, Fausta, Durando, Mario, Latella, Raffaele, Mazzoleni, Marta, Russo, Giuseppe, Ponte, Martino, Valchera, Alessandro, Salustri, Giuseppe, Petritola, Donatella, Costa, Alfredo, Sinforiani, Elena, Cotta, Matteo Ramusino, Pizio, Renato Nicola, Cester, Alberto, Formilan, Marino, Pietro, Bonometto, Carbone, Pasqualina, Cazzaniga, Ilaria, Appollonio, Ildebrando, Cereda, Diletta, Stabile, Andrea, Xhani, Rubjona, Acampora, Roberto, Tremolizzo, Lucio, Federico, Pieruzzi, Antonio, Ciaccio, Valerio, Pontecorvi, Cesare, Burti, Zhirajr, Mokini, Giovanni, Vitale, Maria, Amigoni, Mariaelena, Sparacino, Bottacchi, Edo, Bucciantini, Elisabetta, Di Giovanni, Marco, Franchi, Fabrizio, Lucchetti, Lucio, Mariani, Claudio, Grande, Giulia, Rapazzini, Piero, Marco, Mauri, Romanelli, Giuseppe, Marengoni, Alessandra, Franco, Nicosia, Alessio, Margola, Stefano, Bonardelli, Nicola, Latronico, Laura, Porcella, Nazario, Portolani, Carlo, Concoreggi, Chiara, Grassini, Soccorso, Pronto, Andrea, Salvi, Luca, Bianchetti, Francesca, Spagnoli, Roberto, Apuzzo, Marco, Fontanella, Anna, Ceraso, Francesco, Castelli, Fugazza, Luciano, Guerrini, Chiara, De Paduanis, Giovanna, Iallonardo, Lucia, Palumbo, Pasquale, Zuliani, Giovanni, Ortolani, Beatrice, Capatti, Eleonora, Soavi, Cecilia, Bianchi, Lara, Francesconi, Daniela, Miselli, Agata, Gloria, Brombo, Tommaso, Romagnoli, Chiara, Pazzaglini, Agata, Miselli Maria, Marco, Dall'Agata, Luca, Menozzi, Gianluca, Guerra, Suardi, Teresa, Mazzone, Andrea, Zaccarini, Cinzia, Manuela, Riva, Mirra, Gianluca, Muti, Ettore, Bottura, Renato, Gianpaolo, Moretti, Secreto, Piero, Bisio, Erika, Cecchettani, Marco, Naldi, Tamara, Pallavicino, Alessandra, Pugliese, Michela, Iozzo, Rosaria Cosima, Grassi, Guido, Michele, Bombelli, Raffaella, Dell'Oro, Fosca, Quarti Trevano, Giorgio, Giussani Carlo, Giovanni, Paternò, Ernesto, Contro, Mannironi, Antonio, Giorli, Elisa, Oberti, Sara, Fierro, Brigida, Piccoli, Tommaso, Giacalone, Fabio, Mandas, Antonella, Serchisu, Luca, Costaggiu, Diego, Pinna, Elisa, Orrù, Francesca, Mannai, Martina, Cordioli, Zeno, Pelizzari, Luca, Turcato, Emanuela, Arduini, Pietro, Cacace, Carlo, Chiloiro, Roberta, Cimino, Rosella, Ruberto, Carmen, Giovanni, Ruotolo, Pietro, Gareri, Laura, Greco, Alberto, Castagna, Carmen, Ruberto, Santo, Pierluigi Dal, Andriolli, Antonino, Burattin, Giuseppe, Rossi, Laura, Andreolli Antonino, Cervati Giovanna, Giuseppe, Catalano, Tezza, Fabiana, Maddalena, Pizzardini, Laura, Stievano, Crippa, Patrizia, Aloisio, Paola, Di Monda, Tiziana, Malighetti, Alessandro, Galbassini, Gloria, Salutis, Domus, Ivaldi, Claudio, Russo, Anna Maria, Bennati, Ettore, Pino, Elisabetta, Zavarise, Gianmaria, Pesci, Alberto, Suigo, Giulia, Faverio, Paola, Andrea, Gori, Sabrina, Perego, Zanasi, Massimo, Moniello, Giovanni, Rostagno, Carlo, Cartei, Alessandro, Polidori, Gianluca, Ungar, Andrea, Melis, Maria Ramona, Martellini, Eleonora, Enrico, Mossello, Monica, Torrini, Antonella, Giordano, Giovanna, Leone, Migliorini, Marta, Caramelli, Francesca, Battiston, Bruno, Berardino, Maurizio, Cavallo, Simona, Alessandro, Massè, Anna, Santoro, Lombardi, Bruna, D'Ippolito, Pierpaolo, Furini, Angela, Villani, Daniele, Clara, Raimondi, Guarneri, Massimo, Paolucci, Stefano, Bassi, Andrea, Coiro, Paola, De Angelis, Domenico, Morone, Giovanni, Venturiero, Vincenzo, Palleschi, Lorenzo, Raganato, Paolo, Di Niro, Giuseppina, Rosa, Casini Anna, Loredana, Bove, Imoscopi, Alessandra, Isaia, Giancarlo, Tibaldi, V., Bottignole G, G., Calvi, E., Clementi, C., Zanocchi, M., Agosta, L., Nortarelli, Antonella, Provenzano, Giuseppe, Mari, Daniela, Romano, Federica Ylenia, Rosini, Francesca, Mansi, Marta, Rossi, Silvia, Geriatria, Alex Riccardelli, Inzaghi, Lorenzo, Bonini, Giulia, Rossi, Paolo, Potena, Alfredo, Lichii, Mihaela, Candiani, Tiziana, Grimaldi, William, Bertani, Emiliano, Alessandra, Porta, Calogero, Pietro, Pinto, Daniela, Bernardi, Roberto, Nicolino, Francesco, Galetti, Caterina, Gianstefani, Alice, Giulia, Corvalli, Lorenzo, Mulazzani, Odetti, Patrizio, Monacelli, Fiammetta, Prefumo, Matteo, Fiammetta, Monacelli, Canepa, Marta, Minaglia, Cecilia, Paolisso, Giuseppe, Rizzo, Maria Rosaria, Prestano, Raffaele, Dalise, Anna Maria, Barra, Davide, Bosco, Livio Dal, Asprinio, Vincenzo, Dallape, Luciana, Perina, Elisa, Incalzi, Raffaele Antonelli, Bartoli, Isaura Rossi, Pluderi, Alice, Maina, Antonella, Pecoraro, Elisabetta, Sciarra, Michela, Prudente, Angela, Paola, Maina, Francesca, Mete, Manuel, Ventura, Luisella, Cesari, Maria, Pernigotti Luigi, Tina, Santangelo, Benini, Lucia, Levato, Francesco, Mhiuta, Victor, Alius, Florin, Davidoaia, Diana, Giardini, Vittorio, Garancini, Mattia, Bellamoli, Claudio, Terranova, Luciano, Bozzini, Claudia, Tosoni, Paolo, Provoli, Emma, Cascone, Luisa, Dioli, Andrea, Ferrarin, Gianfranco, Bucci, Adelmo, Bua, Guido, Fenu, Sara, Bianchi, Giovanna, Casella, Silvia, Romano, Valentina, Maurizio, Poli, Mascherona, Ilenia, Belotti, Gloria, Cavaliere, Sabina, Cuni, Estella, Merciuc, Nina, Oberti, Rosella, Veneziani, Silvia, Capoferri, Emanuela, De Bernardi, Elisabetta, Colombo, Katia, Bravi, Marco, Nicoletta, Negrinotti, D'Arcangelo, Paolo, Montenegro, Nicola, Montanari, Roberto, Lamanna, Pierpaolo, Gasperini, Beatrice, Isabella, Montesi, Stefania, Diotallevi, Gaia, Altobelli, Filippo, Calcinaro, Palamà, Chiara, Di Emidio, Chiara, Scarpini, Elio, Arighi, Andrea, Fumagalli, Giorgio, Basilico, Paola, De Amicis Margherita, Migone, Marta, Mancarella, Diletta, Maira, Granata, Antonino, Ranalli, Claudia, Cammilli, Alessandra, Cavallini, Maria Chiara, Tricca, Manola, Natella, Daniela, Gabbani, Luciano, Tesi, Francesca, Martella, Letizia, Imbrici, Rosalba, Guerrini, Gianbattista, Scotuzzi, Anna Maria, Sozzi, Ferdinando, Valenti, Luigi, Chiarello, Antonino, Monia, Monella, Pilotto, Alberto, Prete, Camilla, Senesi, Barbara, Meta, Anna Cristina, Pendenza, Enrico, Pasqualetti, Giuseppe, Polini, Antonio, Tognini, Sara, Ballino, Elena, Cherubini, Antonio, Dell'Aquila, Giuseppina, Gasparrini, Pina Maria, Marotti, Elisabetta, Migale, Monica, Scrimieri, Antonia, Falsetti, Lorenzo, Salvi, Aldo, Toigo, Gabriele, Ceschia, Giuliano, Rosso, Alessia, Tongiorgi, Chiara, Scarpa, Cristina, Maurizio, Pacchioni, De Dominicis, Luigino, Pucci, Eugenio, Renzi, Sara, Cartechini, Elisabetta, Tomassini, Pia Francesca, Del Gobbo, Maurizio, Ugenti, Francesca, Romeo, Pasquale, Nardelli, Anna, Lauretani, Fulvio, Visioli, Sandra, Montanari, Ilaria, Ermini, Francesca, Giordano, Antonio, Pigato, Giorgio, Simeone, Emilio, Barbujani, Mario, Giampieri, Marina, Amoruso, Raffaele, Piccinini, Maristella, Ferrari, Camilla, Gambetti, Claudio, Sfrappini, Mario, Semeraro, Letizia, Striuli, Rinaldo, Mariani, Claudia, Pelliccioni, Giuseppe, Marinelli, Donatella, Fabi, Katia, Rossi, Tommaso, Pesallaccia, Martina, Sabbatini, Debora, Gobbi, Beatrice, Cerqua, Raffaella, Tagliani, Giancarla, Schlauser, Elena, Caser, Luciano, Caramello, Elisa, Sandigliano, Franca, Rosso, Giorgio, Ferrari, Alberto, Bendini, Chiara, Luisa, Davoli Maria, Casella, Monica, Prampolini, Raffaella, Scevola, Moreno, Vitale, Enrico, Roberto, Brugiolo, Carlo, Fagherazzi, Sergio, Fusco, Alberto, Scarpa, Daniela, Zara, Giulia, Bertozzo, Serena, Granziera, Michele, Barazzuol, Maugeri, Domenico, Sorace, Rosaria, Anzaldi, Massimiliano, De Gesu, Roberto, Morrone, Giuseppe, Davolio, Federica, Fabbo, Andrea, Palmieri, Marina, Zoli, Marco, Forti, Paola, Pirazzoli, Luca, Fabbri, Elisa, Terenzi, Laura, Bergolari, Federica, Wenter, Christian, Ruffini, Ingrid, Insam, Miriam, Abraham, Elisabeth, Kirchlechner, Christine, Cucinotta, Domenico, Antonino, Lasco, Basile, Giorgio, Grazia, Arena Maria, Parise, Pasquale, Boccali, Andrea, Amici, Serena, Gambacorta, Maurizia, Lasagni, Anna, Lovati, Roberto, Giovinazzo, Francesca, Kimak, Elzbieta, Zappa, Paolo, Medici, Francesco, Lo Castro, Marika, Mauro, Flavia, De Luca, Alessandro, Sancesario, Giuseppe, Martorana, Alessandro, Scaricamazza, Beatrice, Toniolo, Sofia, Di Lorenzo, Francesco, Liguori, Claudio, Lasco, Antonino, Vita, Natale, Giomi, Mirna, Forte, Floriana, Padovani, Alessandro, Rozzini, Luca, Ceraso, Anna, Salvatore, Caratozzolo, Cottino, Maria, Vitali, Silvia, Marelli, Eleonora, Tripi, Gabriele, Miceli, Salvatore, Urso, Giovanni, Grioni, Giuseppe, Vezzadini, Giuliana, Misaggi, Giulia, Forlani, Chiara, Avanzi, Stefano, Serena, Saulle, Claudia, Casarini, Marilena, Viggiano, Alberto, Luisa, Diego, Ghianda, Alessandro, Giordano, Iemolo, Francesco, Giordano, Antonello, Sanzaro, Enzo, D'Asta, Gabriele, Proietto, Maria, Carnemolla, Anna, Razza, Grazia, Spadaro, Daniela, Bertolotti, Marco, Mussi, Chiara, Neviani, Francesca, Roberto, Chiesa, Valentina, Guerzoni, Linda, Morselli, Francesca, Venturelli, Tarozzi, Adriano, Balestri, Francesca, Mannarino, Giulio, Bigolari, Michela, Natale, Alessia, Grassi, Simona, Bottaro, Cinzia, Stefanelli, Sara, Bovone, Ugo, Tortorolo, Umberto, Quadri, Roberto, Leone, Giuseppe, Ponzetto, Maria, Frasson, Paola, Annoni, Giorgio, Bellelli, Giuseppe, Bruni, Adriana, Confalonieri, Roberto, Corsi, Maurizio, Moretti, Daniela, Teruzzi, Fabiola, Umidi, Simona, Mazzola, Paolo, Perego, Sabrina, Persico, Ilaria, Olivieri, Giulia, Bonfanti, Alessandra, Hajnalka, Szabò, Galeazzi, Marianna, Massariello, Francesca, Anzuini, Alessandra, Caffarra, Paolo, Barocco, Federica, Spallazzi, Marco, Paolo, Ceda Gian, Simonetta, Morganti, Andrea, Artoni, Chioatto, Paolo, Bortolamei, Sandra, Soattin, Lucia, Ruotolo, Giovanni, Beneamino, Borelli, Giuseppe, Barilaro, Bertazzoli, Marco, Rota, Elisabetta, Adobati, Annamaria, Scarpa, Alberto, Granziera, Serena, Zuccher, Paola, Fabbro, Angela Dal, Zara, Daniela, Lo Nigro, Ambra, Franchetti, Lorena, Toniolo, Marika, Marcuzzo, Cinzia, Rollone, Marco, Guerriero, Fabio, Sgarlata, Carmelo, Massè, Alessandro, Zatti, Giovanni, Piatti, Massimiliano, Graci, Jole, Benati, Giuseppe, Boschi, Federica, Biondi, Mario, Fiumi, Nicoletta, Erika, Tamburini, Locatelli, Sergio Mario, Mauri, Sabrina, Beretta, Mauro, Margheritis, Laura, Desideri, Giovanbattista, Liberatore, Ester, Carucci, Anna Cecilia, Bonino, Paolo, Caput, Margherita, Antonietti, Maria Paola, Polistena, Giuseppe, De la Pierre, Franz, Mari, Marcello, Massignani, Paola, Tombesi, Fabio, Selvaggio, Fabio, Verbo, Brunella, Bodoni, Paolo, Marchionni, Niccolò, Mossello, Enrico, Sabatini, Tony, Mussio, Eleonora, Magni, Eugenio, Bianchetti, Angelo, Crucitti, Andrea, Titoldini, Giulia, Cossu, Beatrice, Fascendini, Sara, Licini, Cristina, Tomasoni, Angela, Calderazzo, Massimo, Daniela, Tropiano, Valentina, Laganà, Melotti, Rita Maria, Lilli, Albina, Buda, Simona, Adversi, Marco, Noro, Gabriele, Turco, Renato, Ubezio, M Chiara, Mantovani, Anna Roberta, Viola, Maria Cristina, Serrati, Carlo, Pretta, Stefano, Infante, Maria, Gentile, Simona, Morandi, Alessandro, D'Ambrosio, Viviana, Mazzanti, Paolo, Brambilla, Cristina, Sportelli, Silvia, Platto, Caterina, Faraci, Bianca, Quattrocchi, Daniela, Pernigotti, Luigi Maria, Pisu, Cristina, Sicuro, Francesca, Zagnoni, Piergiuseppe, Ghiglia, Stefania, Mosca, Massimiliano, Corazzin, Ileana, Deola, Mariangela, Biagini, Carlo Adriano, Bencini, Francesca, Cantini, Claudia, Tonon, Elisabetta, Pierinelli, Silvia, Onofrj, Marco, Thomas, Astrid, Filomena, Borbone, Bonanni, Laura, Gabriella, Cacchiò, Comi, Giancarlo, Magnani, Giuseppe, Santangelo, Roberto, Mazzeo, Salvatore, Giuseppe, Magnani, Francesca, Caso, Giordano, Cecchetti, Roberto, Sant'Angelo, Barbieri, Cristina, Giroldi, Liviana, Bandini, Fabio, Masina, Marco, Malservisi, Simona, Cicognani, Annalena, Ricca, Laura, Piccininni, Maristella, Tassinari, Tiziana, Brogi, Davide, Sugo, Annalisa, Alessandra, Filippi, Sonia, Manfredi, Valerio, Vanni, Andrea, Usai Carlo, Enrico, Colombo, Vera, Renna Francesca, Assunta, Sauchella, Gianmaria, Zavarise, Mauro, Prete, Pietro, Bisagni, Roberto, Masini, Salvatore, Carrabetta, Barone, Antonella, Razzano, Monica, Giuseppe, Imperoli, Angela, Bini, Francesco, Serra, Valeria, D'Agostino, Federico, Gianolla, Lucia, Pietrangeli, Antonella, Velardi, Elisabetta, Di Cello, Cristina, Rosati, Nadia, Casali, Maria, Sessa, Luciano, Abruzzi, Chiara, Costanzi, Bini, Paolo, Pignata, Michele, Enrico, Buonagurio, Maria, Vollery, Giovanni, Carrieri, Giorgio, Cioni, Andrea, Toschi, Marco, Metra, Piera, Ranieri, Alberto, Zucchelli, Ceccon, Anna, Magrin, Laura, Marin, Sara, Barbara, Sofia, Marco, Masina, Laura, Ghedini, Matteo, Moroni, Marco, Paragona, Caterina, Pallotti Maria, Carla, Rota, Federica, Gottardi, Clara, Tomasoni, Melania, Cappuccio, Giampaolo, Bonini, Stefano, Guerini, Valeria, Guerini, Lucia, Merla, Giovambattista, Desideri, Ester, Liberatore, Cecilia, Carucci Anna, Maurizio, Tovaglieri, Vera, Renna Frandesca, Nadia, Bongiorni, Grillo, Antonio, Arenare, Francesca, Tonino, Mineo, David, Kanah, Giorgio, Vianello Piero, Ubaldo, Balducci, Vincenzo, Sidoti, Stefano, Montanari, Marino, Formilan, Busonera Flavio, Murelli Teodolindo, Paolo, Albanese, Monica, Maselli, Francesco, Bolzetta, Roberto, Fabris, Paolo, Bonino, DuranteMangoni, Emanuele, Testoni, Michela, Fabio, Di Stefano, Loredana, Seccia, Valeria, Sonzini, Fabiano, Maria, Annabella, Di Giorgio, Salvatore, De Cosmo, Greco, Antonio, Grazia, D'Onofrio, Daniele, Sancarlo, Gianluca, Resta, Renzo, Girardello, Sergio, Minervini, Morena, Boni, Vitali, Mariagrazia, Marina, Pizzoni, Paolo, De Colle, Cristina, Scarpa, Alessandra, Frattola, Orlandini, Francesco, La Regina, Micaela, Desirée, Addesi, Mirella, Filippo, Mario, Bo, Paola, Porrino, Padulo, Filomena, Cristina, Margheriti, Dario, Rolano, Giancarla, Moscatelli, Guido, Radaelli, Elena, Montini, Prete, Cosimo, Marileda, Novello, Igor, Bramuzzo, Nicole, Bertin, Elena, Rinaldo, Paolillo, Ciro, Riccardi, Angela, Claudia, Benedetti, Barbara, Rizzi, Francesca, Montagna, Silvia, Vitali, Chiara, Cutaia, Oliver, Baca, Mauro, Colombo, Eleonora, Marelli, Giuseppe, Procino, Rosaria, Tararà, Maria, Cottino, Davide, Dell'Acqua, Stefania, Cislaghi, Marco, Cairati, Massimo, Porta, Luca, Scaglione, Martina, De Feo, Paola, Vertolli, Lia, Salvati, Sandro, Cinotti, Valentina, De Santis, Erminia, Biferi, Paola, Cheli, Romina, Rebizzo, Minisola, Salvatore, Luciano, Colangelo, Pasquale, Abete, Ilaria, Liguori, Francesca, Curcio, Guglielmo, Spassini, Marco, Engheben, Sara, Rotunno, Paola, Arosio, Claudio, Angelini, Francesco, Reggiani, Alessandro, Cappelli, Simona, Marcheselli, Lara, Fratticci, Paola, Ranzani, Simonetta, Cesarini, Antonella, Cerini, Generoso, Uomo, Fernando, Gallucci, Giuliano, Ceschia, Emanuela, Serra, Mariolina, Sola, Alessandro, Delitala, Chiara, Pes, Giulia, Lobianco, Alessandro, Giani, Famularo, Simone, Sandini, Marta, Pinotti, Enrico, Gianotti, Luca, Antonella, Battuello, Giulia, Pintore, Sante, Giardini, Rossi, Andrea, Rubele, Sofia, Sant, Selena, Marco, Vignati, Danila, Clerici, Fabio, Rosa, Bandirali, Maria Pia, Nicoletta, Cattaneo, Laura, Boffi, Paolo, Tosoni, Luciano, Terranova, Leonello, Avalli, Margherita, Scanziani, Stefania, De Notaris, Pierluigi, Del Santo, Laura, Rossi, Fabiana, Tezza, Giovanna, Cervati, Antonino, Andreolli, Felice, Catalano, Giuseppe, Burattin, Danilo, Fogli, Giovanna, Di Bella, Francesco, Landi, Salini, Sara, Angela, Brunetti Maria, Giorgetta, Cappa, Giovanni, Galvagno, Paola, Cena, Gerardo, Bruno, Silvio, Raspo, Letizia, Semeraro, Sabrina, Pedrotti, Davide, Barra, Rosaria, Rizzo Maria, Maria, Dalise Anna, Raffaele, Prestano, Palmieri, Vincenzo Ostilio, Palasciano, Giuseppe, Belfiore, Anna, Portincasa, Piero, Carlo, Sabbà, Vincenzo, Solfrizzi, Alessia, D'introno, Valiani, Vincenzo, Carolina, Bologna, Tiziana, Ciarambino, Paola, Turelli, Ugo, Pazzaglia, Federica, Rodella, Giacomo, Piana, Castellano, Maurizio, Anna, Garelli, Elisa, Casella, Federica, Campana, Antonietta, Coschignano Maria, Luigi, Marinangeli, Fabio, Lorico, Salvatore, Bazzano, Giuseppe, Menculini, Gelosa, Giorgio, Viviana, Ambrogio Teresa, Piras, Valentina, Andrea, Ciricugno, Alessandra, Bollari, Coen, Daniele, Magliola, Renata, Milanesio, Deborah, Muzzulini, Carlo Lorenzo, Paolo, Fogliacco, Marinella, Turla, Sofia, Cotelli Maria, Marta, Bianchi, Siano, Pietro, Capo, Giuseppe, Napoletano, Rosa, Cecilia, Politi, Mancini, Concetta, Del Buono, Corrado, De Bartolomeo, Giuseppe, Addolorata, Martinelli, Carmen, Cefalogli, Roberto, Cozzi, Giovanni, Virtuani Angelo, Moschettini, Gianfilippo, Franco, Mastroianni, Daniela, Roglia, D'Amico, Gabriella, Mirella, Palella, Endrizzi, Cristina, Trotta, Lucia, Ciarambino, Tiziana, Orazio, Zanetti, Emanuela, Terazzi, Marta, Sacchetti, Thomas, Fleetwood, Giacomo, Tondo, Ignazio, Di Fazio, Andrea, Bruni, Giuseppe, Orsitto, Emanuela, Fabbro, Serena, Amici, Elena, D'Imporzano, Anna, Casanova, Serena, Bertolio, Erika, Nervo, Roberto, Silvestri, Elena, Semproni, Manuela, Pintus, Francesca, Aloe, Angelo, Tagliaccica, Zambon, Antonella, and Di Santo, Simona G.
Published: 2020
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12. Calcium signalling pathways in prostate cancer initiation and progression

Author: Roberto Silvestri, Vanessa Nicolì, Priyadarsini Gangadharannambiar, Francesco Crea, and Martin D. Bootman
Subjects: Urology
Published: 2023
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13. Emotional dysregulation in women with endometriosis with cyclical and non-cyclical chronic pelvic pain

Author: Dulce Carolina, Rodríguez-Lozano, María Del Pilar, Meza-Rodríguez, Olivier Paul, Cruz-Orozco, Brenda, Sánchez-Ramírez, Andrea, Olguin-Ortega, José Roberto, Silvestri-Tomassoni, Guillermo, Corona-Barsse, Luis Fernando, Escobar-Ponce, Juan Mario, Solis-Paredes, Benjamín, Dominguez-Trejo, and Ignacio, Camacho-Arroyo
Subjects: Reproductive Medicine, Obstetrics and Gynecology, General Medicine
Abstract: Background Endometriosis is a pathophysiological condition characterized by glands and stroma outside the uterus in regions such as the bladder, ureter, fallopian tubes, peritoneum, ovaries, and even in extra pelvic sites. One of the main clinical problems of endometriosis is chronic pelvic pain (CPP), which considerably affects the patients’ quality of life. Patients with endometriosis may, cyclically or non-cyclically (80% of cases) experience CPP. High levels of anxiety and depression have been described in patients with endometriosis related to CPP; however, this has not been evaluated in endometriosis women with different types of CPP. Therefore, the research question of this study was whether there is a difference in the emotional dysregulation due to the type of pain experienced by women with endometriosis? Methods This work was performed in the National Institute of Perinatology (INPer) in Mexico City from January 2019 to March 2020 and aimed to determine if there are differences in emotional dysregulation in patients with cyclical and non-cyclical CPP. 49 women from 18 to 52 years-old diagnosed with endometriosis presenting cyclical and non-cyclical CPP answered several batteries made up of Mini-Mental State Examination, Visual Analog Scale, Beck's Depression Inventory, State Trait-Anxiety Inventory, and Generalized Anxiety Inventory. Mann–Whitney U and Student’s t-test for independent samples to compare the difference between groups was used. Relative risk estimation was performed to determine the association between non-cyclical and cyclical CPP with probability of presenting emotional dysregulation. Results We observed that patients with non-cyclical CPP exhibited higher levels of depression and anxiety (trait-state and generalized anxiety) than patients with cyclical pain, p Conclusions Our data suggest that patients with non-cyclical (persistent) CPP present a higher emotional dysregulation than those with cyclical pain.
Published: 2022
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14. Emotional dysregulation in endometriosis women with cyclical and non-cyclical chronic pelvic pain

Author: Dulce Carolina Rodríguez-Lozano, María del Pilar Meza-Rodríguez, Olivier Paul Cruz-Orozco, Brenda Sánchez-Ramírez, Andrea Olguin-Ortega, José Roberto Silvestri-Tomassoni, Guillermo Corona-Barsse, Luis Fernando Escobar-Ponce, Juan Mario Solis-Paredes, Benjamín Dominguez-Trejo, and Ignacio Camacho-Arroyo
Abstract: Background: Endometriosis is a pathophysiological condition characterized by glands and stroma outside the uterus in regions such as the bladder, ureter, fallopian tubes, peritoneum, ovaries, and even in extra pelvic sites. One of the main clinical problems of endometriosis is chronic pelvic pain (CPP), which considerably affects the patients’ quality of life. Patients with endometriosis may, cyclically or non-cyclically (80% of cases) experience CPP. High levels of anxiety and depression have been described in patients with endometriosis related to CPP; however, this has not been evaluated in endometriosis women with different types of CPP. Methods: This work aimed to determine if there are differences in emotional dysregulation in patients with cyclical and non-cyclical CPP. 49 women diagnosed with endometriosis presenting cyclical and non-cyclical CPP answered several batteries made up of Mini-Mental State Examination, Visual Analog Scale, Beck´s Depression Inventory, State Trait-Anxiety Inventory, and Generalized Anxiety Inventory. Results: We observed that patients with non-cyclical CPP exhibited higher levels of depression and anxiety (trait-state and generalized anxiety) than patients with cyclical pain. No differences were observed in pain intensity, but there was a higher probability of developing emotional dysregulation (anxiety or depression) in patients with non-cyclical CPP. No differences were observed in cognitive impairment. Conclusions:Our data suggest that patients with non-cyclical CPP present a higher emotional dysregulation than those with cyclical pain.
Published: 2022
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15. Pro64His (rs4644) polymorphism within galectin-3 is a risk factor of differentiated thyroid carcinoma and affects the transcriptome of thyrocytes engineered via CRISPR/Cas9 system

Author: Alda Corrado, Roberto Giovannoni, Laura Poliseno, Marianna Vitiello, Irene Dell’Anno, Simona Miglietta, Romina Aceto, Federica Gemignani, Monica Evangelista, Roberto Silvestri, Monica Cipollini, Lorenzo Zallocco, Rossella Elisei, Laura Giusti, Benedetta Ricci, Cristina Romei, Sonia Garritano, and Stefano Landi
Subjects: Adult, Male, rs4644, endocrine system, Genotype, Galectin 3, Endocrinology, Diabetes and Metabolism, Biology, Polymorphism, Single Nucleotide, Malignant transformation, Thyroid carcinoma, Transcriptome, Extracellular matrix, Endocrinology, galectin-3, Humans, CRISPR, Genetic Predisposition to Disease, Thyroid Neoplasms, Cas9, differentiated thyroid carcinoma, Alleles, Genetic Association Studies, chemistry.chemical_classification, Middle Aged, respiratory system, transcriptome, RNA-seqfunctional polymorphism, Cell biology, chemistry, Thyroid Epithelial Cells, Galectin-3, Cytoplasm, Case-Control Studies, Female, CRISPR-Cas Systems, Glycoprotein
Abstract: Background: Galectin-3 (LGALS3) is an important glycoprotein involved in the malignant transformation of thyrocytes acting in the extracellular matrix, cytoplasm, and nucleus where it regulates TTF...
Published: 2021

16. Common gene variants within 3'-untranslated regions as modulators of multiple myeloma risk and survival

Author: Annette Juul Vangsted, Daria Zawirska, Vibeke Andersen, Marek Dudziński, Stefano Landi, Agnieszka Druzd-Sitek, Hervé Avet-Loiseau, Katia Beider, Malgorzata Krawczyk-Kulis, Marcin Kruszewski, Roberto Silvestri, Aleksandra Butrym, Jan Maciej Zaucha, Yang Li, Anna Stępień, Mihai G. Netea, Federica Morani, Daniele Campa, María Eugenia Sarasquete, Artur Jurczyszyn, Grzegorz Mazur, Juan Sainz, Rob ter Horst, Krzysztof Jamroziak, Giuseppe Maccari, Norbert Grząśko, Rui Manuel Reis, Malwina Rybicka, Matteo Pelosini, Charles Dumontet, Maria Sole Facioni, Małgorzata Raźny, Federica Gemignani, Marcin Rymko, Arnon Nagler, Judit Várkonyi, Elżbieta Iskierka-Jażdżewska, Gergely Szombath, Herlander Marques, Edyta Subocz, Katalin Kadar, Fabienne Lesueur, Victor Moreno, Federico Canzian, Ombretta Melaiu, Diego Calvetti, Andres Jerez, Ulla Vogel, Olga Ostrovsky, Svend Erik Hove Jacobsen, Joaquin Martinez-Lopez, Angelica Macauda, Ramón García-Sanz, and Marzena Watek
Subjects: Male, Cancer Research, Candidate gene, PROGNOSIS, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], medicine.disease_cause, Germline, susceptibility, single nucleotide polymorphisms, 0302 clinical medicine, Gene Frequency, Risk Factors, 3′-untranslated region, multiple myeloma, overall survival, risk, 3' Untranslated Regions, Multiple myeloma, Settore BIO/18, ASSOCIATION, Middle Aged, 3&#8242, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, Female, KRAS, &#8208, Adult, Genotype, Single-nucleotide polymorphism, Biology, Polymorphism, Single Nucleotide, untranslated region, 03 medical and health sciences, medicine, Humans, Genetic Predisposition to Disease, RNA, Messenger, Gene, Germ-Line Mutation, Aged, Reporter gene, IDENTIFICATION, Three prime untranslated region, medicine.disease, Survival Analysis, POLYMORPHISM, Case-Control Studies, Cancer research
Abstract: Contains fulltext : 232394.pdf (Publisher’s version ) (Closed access) We evaluated the association between germline genetic variants located within the 3'-untranlsated region (polymorphic 3'UTR, ie, p3UTR) of candidate genes involved in multiple myeloma (MM). We performed a case-control study within the International Multiple Myeloma rESEarch (IMMEnSE) consortium, consisting of 3056 MM patients and 1960 controls recruited from eight countries. We selected p3UTR of six genes known to act in different pathways relevant in MM pathogenesis, namely KRAS (rs12587 and rs7973623), VEGFA (rs10434), SPP1 (rs1126772), IRF4 (rs12211228) and IL10 (rs3024496). We found that IL10-rs3024496 was associated with increased risk of developing MM and with a worse overall survival of MM patients. The variant allele was assayed in a vector expressing eGFP chimerized with the IL10 3'-UTR and it was found functionally active following transfection in human myeloma cells. In this experiment, the A-allele caused a lower expression of the reporter gene and this was also in agreement with the in vivo expression of mRNA measured in whole blood as reported in the GTEx portal. Overall, these data are suggestive of an effect of the IL10-rs3024496 SNP on the regulation of IL10 mRNA expression and it could have clinical implications for better characterization of MM patients in terms of prognosis.
Published: 2021
Full Text: View/download PDF

17. Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma

Author: Federica Gemignani, Alda Corrado, Elisa Paolicchi, Antonio Giordano, Roberto Silvestri, Sarah A. Martin, Alessandra Melani, Maria Bottaro, Luca Luzzi, Marcella Barbarino, Ombretta Melaiu, Monica Cipollini, Stefano Landi, and Irene Dell’Anno
Subjects: 0301 basic medicine, Mesothelioma, Antimetabolite, Bisphosphonate, Drug repositioning, Fludarabine, Risedronic acid, medicine.drug_class, Cell Survival, medicine.medical_treatment, Pleural Neoplasms, Antineoplastic Agents, Settore MED/05, Cell Line, 03 medical and health sciences, NT5E, 0302 clinical medicine, medicine, Cytotoxic T cell, Humans, Pharmacology (medical), Cell Proliferation, Pharmacology, Preclinical Studies, business.industry, medicine.disease, Gene Expression Regulation, Neoplastic, 030104 developmental biology, STAT1 Transcription Factor, Oncology, 030220 oncology & carcinogenesis, Cancer research, business, Vidarabine, medicine.drug
Abstract: SummaryObjectives Malignant pleural mesothelioma (MPM) is an occupational disease mainly due to asbestos exposure. Effective therapies for MPM are lacking, making this tumour type a fatal disease. Materials and Methods In order to meet this need and in view of a future “drug repositioning” approach, here we screened five MPM (Mero-14, Mero-25, IST-Mes2, NCI-H28 and MSTO-211H) and one SV40-immortalized mesothelial cell line (MeT-5A) as a non-malignant model, with a library of 1170 FDA-approved drugs. Results Among several potential compounds, we found that fludarabine (F-araA) and, to a lesser extent, risedronic acid (RIS) were cytotoxic in MPM cells, in comparison to the non-malignant Met-5A cells. In particular, F-araA reduced the proliferation and the colony formation ability of the MPM malignant cells, in comparison to the non-malignant control cells, as demonstrated by proliferation and colony formation assays, in addition to measurement of the phospho-ERK/total-ERK ratio. We have shown that the response to F-araA was not dependent upon the expression of DCK and NT5E enzymes, nor upon their functional polymorphisms (rs11544786 and rs2295890, respectively). Conclusion This drug repositioning screening approach has identified that F-araA could be therapeutically active against MPM cells, in addition to other tumour types, by inhibiting STAT1 expression and nucleic acids synthesis. Further experiments are required to fully investigate this.
Published: 2021

18. A Drug Screening Revealed Novel Potential Agents against Malignant Pleural Mesothelioma

Author: Irene Dell’Anno, Alessandra Melani, Sarah A. Martin, Marcella Barbarino, Roberto Silvestri, Monica Cipollini, Antonio Giordano, Luciano Mutti, Andrea Nicolini, Luca Luzzi, Raffaele Aiello, Federica Gemignani, and Stefano Landi
Subjects: Cancer Research, malignant pleural mesothelioma, thonzonium bromide, cephalomannine, alexidine, ouabain, emetine, drug repositioning, Oncology
Abstract: The lack of effective therapies remains one of the main challenges for malignant pleural mesothelioma (MPM). In this perspective, drug repositioning could accelerate the identification of novel treatments. We screened 1170 FDA-approved drugs on a SV40-immortalized mesothelial (MeT-5A) and five MPM (Mero-14, Mero-25, IST-Mes2, NCI-H28 and MSTO-211H) cell lines. Biological assays were carried out for 41 drugs, showing the highest cytotoxicity and for whom there were a complete lack of published literature in MPM. Cytotoxicity and caspase activation were evaluated with commercially available kits and cell proliferation was assayed using MTT assay and by clonogenic activity with standard protocols. Moreover, the five most effective drugs were further evaluated on patient-derived primary MPM cell lines. The most active molecules were cephalomannine, ouabain, alexidine, thonzonium bromide, and emetine. Except for alexidine, these drugs inhibited the clonogenic ability and caspase activation in all cancer lines tested. The proliferation was inhibited also on an extended panel of cell lines, including primary MPM cells. Thus, we suggest that cephalomannine, ouabain, thonzonium bromide, and emetine could represent novel candidates to be repurposed for improving the arsenal of therapeutic weapons in the fight against MPM.
Published: 2022
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19. Spettri di Clint : L'America del mito nell'opera di Eastwood

Author: Mariuccia Ciotta, Roberto Silvestri, Mariuccia Ciotta, and Roberto Silvestri
Abstract: Messo all'indice per la violenza del suo personaggio nella serie dell'ispettore Callaghan, Clint Eastwood è stato celebrato dalla critica solo nel passaggio del secolo. Fenomenale davanti alla macchina da presa, nei panni del regista ha saputo misurarsi coraggiosamente con i grandi drammi della storia, senza aderire a estetiche alla moda. Il suo cinema, rivoluzionario sin dagli esordi con Sergio Leone, ha creato scompiglio nel patinato mondo di Hollywood, capovolgendo come un antieroe la leggenda del West e opponendole un altro volto, diviso tra furia selvaggia e sogno americano. Controcorrente per vocazione, ha riscritto il canone di thriller, spy-story, commedia, western e melodramma attraverso il lavoro della sua factory, la casa di produzione Malpaso, di cui protegge strenuamente l'indipendenza creativa. Nessuno più di Clint Eastwood ha indagato e sviscerato con tanto disincanto i miti e i conflitti dell'America, cantandone l'innocenza perduta e dissacrandone l'eroismo, la mascolinità, il razzismo. Parlare oggi di Eastwood è parlare di un mondo altro e sempre attuale: Mariuccia Ciotta e Roberto Silvestri ne ricostruiscono la figura a tutto tondo, tracciando una cartografia immaginaria ispirata a Mission Ranch, una sorta di Eastwoodland, dove il cineasta ha voluto ricreare un ambiente artistico perfetto, all'incrocio tra barbarie e civilizzazione. Clint Eastwood si aggira come uno spettro senza riposo sulle macerie di un'innocenza perduta. Forse l'angelo dell'Apocalisse non viene dal cielo, ma è appena uscito dal fango. Ogni spettatore di Ispettore Callaghan: il caso Scorpio è tuo! entrava in sala con uno sguardo innocente. Un'innocenza che presto avremmo perso. QUENTIN TARANTINO
Published: 2023

20. Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

Author: Claudia Bello-Alvarez, J Roberto Silvestri-Tomassoni, Christian Reyes-Mayoral, Andrea Alicia Olguín-Ortega, Luis Fernando Escobar-Ponce, Ana Lorena Hermenegildo-Molina, Mario Solis-Paredes, Mauricio Osorio-Caballero, Elizabeth García-Gómez, Ignacio Camacho-Arroyo, Edgar Ricardo Vázquez-Martínez, Sandra Parra-Hernández, Oliver Paul Cruz-Orozco, Luis A Hernández-López, Guadalupe Estrada-Gutierrez, Brenda Sánchez-Ramírez, and Marco Cerbón
Subjects: 0301 basic medicine, Adult, Article Subject, Endometriosis, Down-Regulation, Biology, Endometrium, General Biochemistry, Genetics and Molecular Biology, Andrology, 03 medical and health sciences, 0302 clinical medicine, Western blot, Progesterone receptor, Gene expression, medicine, Humans, Receptor, Messenger RNA, 030219 obstetrics & reproductive medicine, General Immunology and Microbiology, medicine.diagnostic_test, urogenital system, Cell Membrane, Membrane Proteins, General Medicine, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Medicine, Female, Receptors, Progesterone, Intracellular, Research Article
Abstract: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of mPR members in the eutopic and ectopic endometrium of women with endometriosis. Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR6 (mPRδ) at mRNA and protein levels was evaluated by RT-qPCR and Western blot, whereas PAQR5 (mPRγ) gene expression was evaluated by RT-qPCR. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey’s multiple comparisons test with a confidence interval of 95 %. The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.
Published: 2020
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21. The Unexploited Potential of Small-Scale Fisheries in Italy: Analysis and Perspectives on the Status and Resilience of a Neglected Fishery Sector

Author: Romano T. Baino, Saša Raicevich, Pietro Battaglia, M. Dubois, Mario Sbrana, Roberto Silvestri, Fabio Grati, Massimo Spagnolo, Otello Giovanardi, Paolo Sartor, Franco Andaloro, and Teresa Romeo
Subjects: 0106 biological sciences, 010604 marine biology & hydrobiology, media_common.quotation_subject, Fishing, Citizen journalism, Legislation, 04 agricultural and veterinary sciences, 01 natural sciences, Fishery, Competition (economics), Scale (social sciences), 040102 fisheries, 0401 agriculture, forestry, and fisheries, Ecosystem, 14. Life underwater, Psychological resilience, Business, Rivalry, media_common
Abstract: Small-scale fisheries represent one of the most important fishing sectors in Italy, comprising 60% of Italian fishing vessels, half the fishers and 16% of national landings. Despite their high social, economic and cultural value, they have been neglected by national and international policies, resulting in a poor outlook for the near future. In this chapter, we provide an overview of recent trends, the status of small-scale fisheries in Italy and major, current threats to the sector. These include: competition from large-scale fisheries, rivalry with illegal, unreported and unregulated fishing, recreational fisheries, ecological changes and the degradation of ecosystems. The evolution of international and national legislation affecting small-scale fisheries is also outlined, with examples of opportunities and challenges in two complementary case studies from the Tyrrhenian and Ionian Seas. The analysis is mainly focused on participatory, bottom-up management tools (local management plans and co-management schemes). The resilience of small-scale fisheries’ socio-ecological systems, mainly related to fishers’ capability of adapting their strategies to multiple sets of conditions over time and across areas, is also discussed. We conclude our analysis by investigating actions which could be implemented to allow the unexploited economic, social and cultural potential of small-scale fisheries in Italy to be developed.
Published: 2020
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22. Eif4g1 and ran as possible drivers for malignant pleural mesothelioma

Author: Stefano Landi, Alda Corrado, Alessandra Melani, Maria Bottaro, Loredana Migliore, Roberto Silvestri, Ombretta Melaiu, Elisa Barone, Silvia Agostini, Monica Cipollini, Luca Luzzi, Antonio Giordano, Irene Dell’Anno, Federica Gemignani, Calogerina Catalano, and Marcella Barbarino
Subjects: 0301 basic medicine, Carcinogenesis, MPM, medicine.disease_cause, Settore MED/05, Epithelium, lcsh:Chemistry, 0302 clinical medicine, RNA, Small Interfering, lcsh:QH301-705.5, Spectroscopy, Caspase, EIF4G1, biology, General Medicine, beta Karyopherins, Computer Science Applications, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, importazole, Pleural Neoplasms, Cancer driving gene, Importazole, RAN, SiRNA, cancer driving gene, Catalysis, Article, Inorganic Chemistry, Small Molecule Libraries, 03 medical and health sciences, Downregulation and upregulation, Cell Line, Tumor, medicine, Gene silencing, Humans, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Cell growth, Organic Chemistry, Mesothelioma, Malignant, Cancer, medicine.disease, 030104 developmental biology, ran GTP-Binding Protein, lcsh:Biology (General), lcsh:QD1-999, Cell culture, siRNA, Ran, Cancer research, biology.protein, Quinazolines, Eukaryotic Initiation Factor-4G
Abstract: For malignant pleural mesothelioma (MPM) novel therapeutic strategies are urgently needed. In a previous study, we identified 51 putative cancer genes over-expressed in MPM tissues and cell lines. Here, we deepened the study on nine of them (ASS1, EIF4G1, GALNT7, GLUT1, IGF2BP3 (IMP3), ITGA4, RAN, SOD1, and THBS2) to ascertain whether they are truly mesothelial cancer driver genes (CDGs) or genes overexpressed in an adaptive response to the tumoral progression (&ldquo, passenger genes&rdquo, ). Through a fast siRNA-based screening, we evaluated the consequences of gene depletion on migration, proliferation, colony formation capabilities, and caspase activities of four MPM (Mero-14, Mero-25, IST-Mes2, and NCI-H28) and one SV40-immortalized mesothelial cell line (MeT-5A) as a non-malignant model. The depletion of EIF4G1 and RAN significantly reduced cell proliferation and colony formation and increased caspase activity. In particular, the findings for RAN resemble those observed for other types of cancer. Thus, we evaluated the in vitro effects of importazole (IPZ), a small molecule inhibitor of the interaction between RAN and importin-&beta, We showed that IPZ could have effects similar to those observed following RAN gene silencing. We also found that primary cell lines from one out of three MPM patients were sensitive to IPZ. As EIF4G1 and RAN deserve further investigation with additional in vitro and in vivo studies, they emerged as promising CDGs, suggesting that their upregulation could play a role in mesothelial tumorigenesis and aggressiveness. Furthermore, present data propose the molecular pathways dependent on RAN as a putative pharmacological target for MPM patients in the view of a future personalized medicine.
Published: 2020

23. Understanding Factors Associated With Psychomotor Subtypes of Delirium in Older Inpatients With Dementia

Author: Morandi, Alessandro, primary, Zambon, Antonella, additional, Di Santo, Simona G., additional, Mazzone, Andrea, additional, Cherubini, Antonio, additional, Mossello, Enrico, additional, Bo, Mario, additional, Marengoni, Alessandra, additional, Bellelli, Giuseppe, additional, Rispoli, Vincenzo, additional, Malara, Alba, additional, Spadea, Fausto, additional, Di Cello, Serena, additional, Ceravolo, Francesco, additional, Fabiano, Francesco, additional, Chiaradia, Giuseppe, additional, Gabriele, Amedeo, additional, Lenino, Peluso, additional, Andrea, Taristano, additional, Settembrini, Vincenzo, additional, Capomolla, Domenico, additional, Citrino, Antonella, additional, Scriva, Antonietta, additional, Bruno, Irene, additional, Secchi, Roberto, additional, De Martino, Eugenio, additional, Muccinelli, Roberto, additional, Lupi, Gerardo, additional, Paonessa, Patrizio, additional, Fabbri, Andrea, additional, Passuti, Maria Teresa, additional, Castellari, Sofia, additional, Po, Andrea, additional, Gaggioli, Guido, additional, Varesi, Massimo, additional, Moneti, Paolo, additional, Capurso, Sebastiano, additional, Latini, Vincenzo, additional, Ghidotti, Stefano, additional, Riccardelli, Francesco, additional, Macchi, Maurizio, additional, Rigo, Rosaria, additional, Claudio, Pascale, additional, Angelo, Bosio, additional, Flavio, Cerrato, additional, Benedetta, Bardelli, additional, Boffelli, Stefano, additional, Cassinadri, Angela, additional, Franzoni, Simone, additional, Spazzini, Elena, additional, Andretto, Daniela, additional, Tonini, Gabriele, additional, Andreani, Laura, additional, Coralli, Mirco, additional, Balotta, Antonio, additional, Cancelliere, Roberto, additional, Ballardini, Giorgio, additional, Simoncelli, Margherita, additional, Mancini, Annarita, additional, Strazzacapa, Mara, additional, Fabio, Stefano, additional, De Filippi, Francesco, additional, Giudice, Chiara, additional, Dentizzi, Cosimo, additional, Azzini, Margherita, additional, Cazzadori, Marco, additional, Mastroeni, Valeria, additional, Bertassello, Paolo, additional, Claudia Benati, Helena Santana, additional, Nesta, Elisa, additional, Tobaldini, Chiara, additional, Guerini, Fabio, additional, Elena, Tambara, additional, Mombelloni, Paolo, additional, Fontanini, Fulvio, additional, Gabriella, Lassa, additional, Pizzorni, Crosio, additional, Oliverio, Martina, additional, Del Grosso, Luciano Luca, additional, Giavedoni, Cristina, additional, Bidoli, Giuliano, additional, Mazzei, Bruno, additional, Corsonello, Andrea, additional, Fusco, Sergio, additional, Vena, Silvio, additional, De Vuono, Tommaso, additional, Maiuri, Giorgio, additional, Luca, Fimognari Filippo, additional, Andrea, Arone, additional, Giovanni, Sgrò, additional, Rossella, Nicolazzo, additional, Castegnaro, Eugenio, additional, De Rosa, Salvatore, additional, Sechi, Rossella Bazzano, additional, Benvenuti, Enrico, additional, Del Lungo, Ilaria, additional, Giardini, Sante, additional, Giulietti, Chiara, additional, Mauro, Di Bari, additional, Eleonora, Barghini, additional, Martina, Paoli, additional, Irene, Fiordelli, additional, Riccardo, Barucci, additional, Federica, Sgrilli, additional, Ilaria, Del Lungo, additional, Bertoletti, Erik, additional, D'Amico, Ferdinando, additional, Caronzolo, Francesco, additional, Grippa, Alessandro, additional, Lombardo, Giuseppina, additional, Pipicella, Tiziana, additional, Antonino, Saitta, additional, Francesco, Corica, additional, Valeria, Prestipino Giarritta, additional, Daniela, Larosa, additional, Domenico, Cucinotta, additional, Giorgio, Basile, additional, March, Albert, additional, Nitti, Maria Teresa, additional, Felici, Alessandro, additional, Pavan, Silvia, additional, Piazzani, Fabrizio, additional, Lunelli, Alessandra, additional, Dimori, Sergio, additional, Margotta, Alessandro, additional, Soglia, Tiziano, additional, Postacchini, Demetrio, additional, Brunelli, Roberto, additional, Santini, Silvia, additional, Francavilla, Monia, additional, Macchiati, Ilenia, additional, Sorvillo, Francesca, additional, Giuli, Cinzia, additional, Mecocci, Patrizia, additional, Longo, Annalisa, additional, Perticone, Francesco, additional, Addesi, Desireè, additional, Rosa, Paola Cerra, additional, Bencardino, Giuseppe, additional, Falbo, Tania, additional, Grillo, Nadia, additional, Marco, Filice, additional, Mirella, Flippo, additional, Fantò, Fausto, additional, Isaia, Gianluca, additional, Pezzilli, Stella, additional, Bergamo, Daniele, additional, Furno, Elisabetta, additional, Rrodhe, Sokol, additional, Lucarini, Simonetta, additional, Dijk, Babette, additional, Dall'Acqua, Francesca, additional, Cappelletto, Francesco, additional, Calvani, Donatella, additional, Becheri, Dimitri, additional, Giuseppe, Mottino, additional, Costanza, Mitidieri, additional, Vito, Antonio, additional, Francesca, Bartalucci, additional, Magherini, Lorenza, additional, Novella, Malin, additional, Franca, Boni, additional, Lucia Gambardella, Paoli Martina, additional, Valente, Carlo, additional, Ilaria, Bracali, additional, Alice, Foschini, additional, Porrino, Paola, additional, Ceci, Giacomo, additional, Giuliana, Bottignole, additional, Michela, Tibaldi, additional, Eleonora, Coppo, additional, Ettore, Evaristo, additional, Camellini, Cinzia, additional, Servello, Adriana, additional, Grassi, Alessandro, additional, Rozzini, Renzo, additional, Tironi, Sara, additional, Grassi, Maria Grazia, additional, Troisi, Elio, additional, Carlo, Caltagirone, additional, Simona Gabriella, Di Santo, additional, Flaminia, Franchini, additional, Federica, Ratto, additional, Beatrice, Pavoni, additional, Sofia, Toniolo, additional, Gabutto, Anna, additional, Quazzo, Loredana, additional, Rosatello, Annalisa, additional, Suraci, Domenico, additional, Tagliabue, Benedetta, additional, Perrone, Chiara, additional, Ferrara, Lucia, additional, Castagna, Alberto, additional, Tremolada, Maria Luisa, additional, Giuseppe, Castoldi, additional, Stefano, Barbero, additional, Davide, Oltramonti, additional, Piano, Simonetta, additional, Serviddio, Gaetano, additional, Lo Buglio, Aurelio, additional, Gurrera, Tiziana, additional, Merlo, Valeria, additional, Rovai, Carla, additional, Cotroneo, Antonino Maria, additional, Carlucci, Rosaria, additional, Abbaldo, Anna, additional, Monzani, Fabio, additional, Qasem, Ahmad Amedeo, additional, Bini, Giacomo, additional, Tafuto, Silvia, additional, Galli, Giovanni, additional, Bruni, Amalia Cecilia, additional, Mancuso, Giovanna, additional, Mancuso, Gerardo, additional, Calipari, Daniela, additional, Giuseppe Massimiliano, De Luca, additional, Bernardini, Bruno, additional, Corsini, Carla, additional, Michele, Ciccarelli, additional, Sara, Dal Farra, additional, Cagnin, Annachiara, additional, Fragiacomo, Federica, additional, Pompanin, Sara, additional, Piero, Amodio, additional, Marco, Cagnin, additional, Zurlo, Amedeo, additional, Guerra, Gianluca, additional, Pala, Marco, additional, Menozzi, Luca, additional, Gatti, Chiara Delli, additional, Magon, Stefania, additional, Roberto, Manfredini, additional, Alfredo, De Giorgi, additional, Fabio, Fabbian, additional, Ruana, Tiseo, additional, Elisa, Misurati, additional, Benedetta, Boari, additional, Christian, Molino, additional, Marco, Pala, additional, Massimo, Gallerani, additional, Di Francesco, Vincenzo, additional, Faccioli, Silvia, additional, Pellizzari, Luca, additional, Giorgia, Fontana, additional, Barbagallo, Giuseppe, additional, Lunardelli, Maria Lia, additional, Martini, Emilio, additional, Ferrari, Eleonora, additional, Macchiarulo, Maria, additional, Corneli, Maria, additional, Bacci, Monica, additional, Battaglia, Giuseppe, additional, Anastasio, Luigi, additional, Lo Storto, Mario Sostene, additional, Seresin, Chiara, additional, Simonato, Matteo, additional, Loreggian, Michele, additional, Cestonaro, Fausta, additional, Durando, Mario, additional, Latella, Raffaele, additional, Mazzoleni, Marta, additional, Russo, Giuseppe, additional, Ponte, Martino, additional, Valchera, Alessandro, additional, Salustri, Giuseppe, additional, Petritola, Donatella, additional, Costa, Alfredo, additional, Sinforiani, Elena, additional, Cotta, Matteo Ramusino, additional, Pizio, Renato Nicola, additional, Cester, Alberto, additional, Formilan, Marino, additional, Pietro, Bonometto, additional, Carbone, Pasqualina, additional, Cazzaniga, Ilaria, additional, Appollonio, Ildebrando, additional, Cereda, Diletta, additional, Stabile, Andrea, additional, Xhani, Rubjona, additional, Acampora, Roberto, additional, Tremolizzo, Lucio, additional, Federico, Pieruzzi, additional, Antonio, Ciaccio, additional, Valerio, Pontecorvi, additional, Cesare, Burti, additional, Zhirajr, Mokini, additional, Giovanni, Vitale, additional, Maria, Amigoni, additional, Mariaelena, Sparacino, additional, Bottacchi, Edo, additional, Bucciantini, Elisabetta, additional, Di Giovanni, Marco, additional, Franchi, Fabrizio, additional, Lucchetti, Lucio, additional, Mariani, Claudio, additional, Grande, Giulia, additional, Rapazzini, Piero, additional, Marco, Mauri, additional, Romanelli, Giuseppe, additional, Franco, Nicosia, additional, Alessio, Margola, additional, Stefano, Bonardelli, additional, Nicola, Latronico, additional, Laura, Porcella, additional, Nazario, Portolani, additional, Carlo, Concoreggi, additional, Chiara, Grassini, additional, Soccorso, Pronto, additional, Andrea, Salvi, additional, Luca, Bianchetti, additional, Francesca, Spagnoli, additional, Roberto, Apuzzo, additional, Marco, Fontanella, additional, Anna, Ceraso, additional, Francesco, Castelli, additional, Fugazza, Luciano, additional, Guerrini, Chiara, additional, De Paduanis, Giovanna, additional, Iallonardo, Lucia, additional, Palumbo, Pasquale, additional, Zuliani, Giovanni, additional, Ortolani, Beatrice, additional, Capatti, Eleonora, additional, Soavi, Cecilia, additional, Bianchi, Lara, additional, Francesconi, Daniela, additional, Miselli, Agata, additional, Gloria, Brombo, additional, Tommaso, Romagnoli, additional, Chiara, Pazzaglini, additional, Agata, Miselli Maria, additional, Marco, Dall'Agata, additional, Luca, Menozzi, additional, Gianluca, Guerra, additional, Suardi, Teresa, additional, Zaccarini, Cinzia, additional, Manuela, Riva, additional, Mirra, Gianluca, additional, Muti, Ettore, additional, Bottura, Renato, additional, Gianpaolo, Moretti, additional, Secreto, Piero, additional, Bisio, Erika, additional, Cecchettani, Marco, additional, Naldi, Tamara, additional, Pallavicino, Alessandra, additional, Pugliese, Michela, additional, Iozzo, Rosaria Cosima, additional, Grassi, Guido, additional, Michele, Bombelli, additional, Raffaella, Dell'Oro, additional, Fosca, Quarti Trevano, additional, Giorgio, Giussani Carlo, additional, Giovanni, Paternò, additional, Ernesto, Contro, additional, Mannironi, Antonio, additional, Giorli, Elisa, additional, Oberti, Sara, additional, Fierro, Brigida, additional, Piccoli, Tommaso, additional, Giacalone, Fabio, additional, Mandas, Antonella, additional, Serchisu, Luca, additional, Costaggiu, Diego, additional, Pinna, Elisa, additional, Orrù, Francesca, additional, Mannai, Martina, additional, Cordioli, Zeno, additional, Pelizzari, Luca, additional, Turcato, Emanuela, additional, Arduini, Pietro, additional, Cacace, Carlo, additional, Chiloiro, Roberta, additional, Cimino, Rosella, additional, Ruberto, Carmen, additional, Giovanni, Ruotolo, additional, Pietro, Gareri, additional, Laura, Greco, additional, Alberto, Castagna, additional, Carmen, Ruberto, additional, Santo, Pierluigi Dal, additional, Andriolli, Antonino, additional, Burattin, Giuseppe, additional, Rossi, Laura, additional, Andreolli Antonino, Cervati Giovanna, additional, Giuseppe, Catalano, additional, Tezza, Fabiana, additional, Maddalena, Pizzardini, additional, Laura, Stievano, additional, Crippa, Patrizia, additional, Aloisio, Paola, additional, Di Monda, Tiziana, additional, Malighetti, Alessandro, additional, Galbassini, Gloria, additional, Salutis, Domus, additional, Ivaldi, Claudio, additional, Russo, Anna Maria, additional, Bennati, Ettore, additional, Pino, Elisabetta, additional, Zavarise, Gianmaria, additional, Pesci, Alberto, additional, Suigo, Giulia, additional, Faverio, Paola, additional, Andrea, Gori, additional, Sabrina, Perego, additional, Zanasi, Massimo, additional, Moniello, Giovanni, additional, Rostagno, Carlo, additional, Cartei, Alessandro, additional, Polidori, Gianluca, additional, Ungar, Andrea, additional, Melis, Maria Ramona, additional, Martellini, Eleonora, additional, Enrico, Mossello, additional, Monica, Torrini, additional, Antonella, Giordano, additional, Giovanna, Leone, additional, Migliorini, Marta, additional, Caramelli, Francesca, additional, Battiston, Bruno, additional, Berardino, Maurizio, additional, Cavallo, Simona, additional, Alessandro, Massè, additional, Anna, Santoro, additional, Lombardi, Bruna, additional, D'Ippolito, Pierpaolo, additional, Furini, Angela, additional, Villani, Daniele, additional, Clara, Raimondi, additional, Guarneri, Massimo, additional, Paolucci, Stefano, additional, Bassi, Andrea, additional, Coiro, Paola, additional, De Angelis, Domenico, additional, Morone, Giovanni, additional, Venturiero, Vincenzo, additional, Palleschi, Lorenzo, additional, Raganato, Paolo, additional, Di Niro, Giuseppina, additional, Rosa, Casini Anna, additional, Loredana, Bove, additional, Imoscopi, Alessandra, additional, Isaia, Giancarlo, additional, Tibaldi, V., additional, Bottignole G, G., additional, Calvi, E., additional, Clementi, C., additional, Zanocchi, M., additional, Agosta, L., additional, Nortarelli, Antonella, additional, Provenzano, Giuseppe, additional, Mari, Daniela, additional, Romano, Federica Ylenia, additional, Rosini, Francesca, additional, Mansi, Marta, additional, Rossi, Silvia, additional, Geriatria, Alex Riccardelli, additional, Inzaghi, Lorenzo, additional, Bonini, Giulia, additional, Rossi, Paolo, additional, Potena, Alfredo, additional, Lichii, Mihaela, additional, Candiani, Tiziana, additional, Grimaldi, William, additional, Bertani, Emiliano, additional, Alessandra, Porta, additional, Calogero, Pietro, additional, Pinto, Daniela, additional, Bernardi, Roberto, additional, Nicolino, Francesco, additional, Galetti, Caterina, additional, Gianstefani, Alice, additional, Giulia, Corvalli, additional, Lorenzo, Mulazzani, additional, Odetti, Patrizio, additional, Monacelli, Fiammetta, additional, Prefumo, Matteo, additional, Fiammetta, Monacelli, additional, Canepa, Marta, additional, Minaglia, Cecilia, additional, Paolisso, Giuseppe, additional, Rizzo, Maria Rosaria, additional, Prestano, Raffaele, additional, Dalise, Anna Maria, additional, Barra, Davide, additional, Bosco, Livio Dal, additional, Asprinio, Vincenzo, additional, Dallape, Luciana, additional, Perina, Elisa, additional, Incalzi, Raffaele Antonelli, additional, Bartoli, Isaura Rossi, additional, Pluderi, Alice, additional, Maina, Antonella, additional, Pecoraro, Elisabetta, additional, Sciarra, Michela, additional, Prudente, Angela, additional, Paola, Maina, additional, Francesca, Mete, additional, Manuel, Ventura, additional, Luisella, Cesari, additional, Maria, Pernigotti Luigi, additional, Tina, Santangelo, additional, Benini, Lucia, additional, Levato, Francesco, additional, Mhiuta, Victor, additional, Alius, Florin, additional, Davidoaia, Diana, additional, Giardini, Vittorio, additional, Garancini, Mattia, additional, Bellamoli, Claudio, additional, Terranova, Luciano, additional, Bozzini, Claudia, additional, Tosoni, Paolo, additional, Provoli, Emma, additional, Cascone, Luisa, additional, Dioli, Andrea, additional, Ferrarin, Gianfranco, additional, Bucci, Adelmo, additional, Bua, Guido, additional, Fenu, Sara, additional, Bianchi, Giovanna, additional, Casella, Silvia, additional, Romano, Valentina, additional, Maurizio, Poli, additional, Mascherona, Ilenia, additional, Belotti, Gloria, additional, Cavaliere, Sabina, additional, Cuni, Estella, additional, Merciuc, Nina, additional, Oberti, Rosella, additional, Veneziani, Silvia, additional, Capoferri, Emanuela, additional, De Bernardi, Elisabetta, additional, Colombo, Katia, additional, Bravi, Marco, additional, Nicoletta, Negrinotti, additional, D'Arcangelo, Paolo, additional, Montenegro, Nicola, additional, Montanari, Roberto, additional, Lamanna, Pierpaolo, additional, Gasperini, Beatrice, additional, Isabella, Montesi, additional, Stefania, Diotallevi, additional, Gaia, Altobelli, additional, Filippo, Calcinaro, additional, Palamà, Chiara, additional, Di Emidio, Chiara, additional, Scarpini, Elio, additional, Arighi, Andrea, additional, Fumagalli, Giorgio, additional, Basilico, Paola, additional, De Amicis Margherita, Migone, additional, Marta, Mancarella, additional, Diletta, Maira, additional, Granata, Antonino, additional, Ranalli, Claudia, additional, Cammilli, Alessandra, additional, Cavallini, Maria Chiara, additional, Tricca, Manola, additional, Natella, Daniela, additional, Gabbani, Luciano, additional, Tesi, Francesca, additional, Martella, Letizia, additional, Imbrici, Rosalba, additional, Guerrini, Gianbattista, additional, Scotuzzi, Anna Maria, additional, Sozzi, Ferdinando, additional, Valenti, Luigi, additional, Chiarello, Antonino, additional, Monia, Monella, additional, Pilotto, Alberto, additional, Prete, Camilla, additional, Senesi, Barbara, additional, Meta, Anna Cristina, additional, Pendenza, Enrico, additional, Pasqualetti, Giuseppe, additional, Polini, Antonio, additional, Tognini, Sara, additional, Ballino, Elena, additional, Dell'Aquila, Giuseppina, additional, Gasparrini, Pina Maria, additional, Marotti, Elisabetta, additional, Migale, Monica, additional, Scrimieri, Antonia, additional, Falsetti, Lorenzo, additional, Salvi, Aldo, additional, Toigo, Gabriele, additional, Ceschia, Giuliano, additional, Rosso, Alessia, additional, Tongiorgi, Chiara, additional, Scarpa, Cristina, additional, Maurizio, Pacchioni, additional, De Dominicis, Luigino, additional, Pucci, Eugenio, additional, Renzi, Sara, additional, Cartechini, Elisabetta, additional, Tomassini, Pia Francesca, additional, Del Gobbo, Maurizio, additional, Ugenti, Francesca, additional, Romeo, Pasquale, additional, Nardelli, Anna, additional, Lauretani, Fulvio, additional, Visioli, Sandra, additional, Montanari, Ilaria, additional, Ermini, Francesca, additional, Giordano, Antonio, additional, Pigato, Giorgio, additional, Simeone, Emilio, additional, Barbujani, Mario, additional, Giampieri, Marina, additional, Amoruso, Raffaele, additional, Piccinini, Maristella, additional, Ferrari, Camilla, additional, Gambetti, Claudio, additional, Sfrappini, Mario, additional, Semeraro, Letizia, additional, Striuli, Rinaldo, additional, Mariani, Claudia, additional, Pelliccioni, Giuseppe, additional, Marinelli, Donatella, additional, Fabi, Katia, additional, Rossi, Tommaso, additional, Pesallaccia, Martina, additional, Sabbatini, Debora, additional, Gobbi, Beatrice, additional, Cerqua, Raffaella, additional, Tagliani, Giancarla, additional, Schlauser, Elena, additional, Caser, Luciano, additional, Caramello, Elisa, additional, Sandigliano, Franca, additional, Rosso, Giorgio, additional, Ferrari, Alberto, additional, Bendini, Chiara, additional, Luisa, Davoli Maria, additional, Casella, Monica, additional, Prampolini, Raffaella, additional, Scevola, Moreno, additional, Vitale, Enrico, additional, Roberto, Brugiolo, additional, Carlo, Fagherazzi, additional, Sergio, Fusco, additional, Alberto, Scarpa, additional, Daniela, Zara, additional, Giulia, Bertozzo, additional, Serena, Granziera, additional, Michele, Barazzuol, additional, Maugeri, Domenico, additional, Sorace, Rosaria, additional, Anzaldi, Massimiliano, additional, De Gesu, Roberto, additional, Morrone, Giuseppe, additional, Davolio, Federica, additional, Fabbo, Andrea, additional, Palmieri, Marina, additional, Zoli, Marco, additional, Forti, Paola, additional, Pirazzoli, Luca, additional, Fabbri, Elisa, additional, Terenzi, Laura, additional, Bergolari, Federica, additional, Wenter, Christian, additional, Ruffini, Ingrid, additional, Insam, Miriam, additional, Abraham, Elisabeth, additional, Kirchlechner, Christine, additional, Cucinotta, Domenico, additional, Antonino, Lasco, additional, Basile, Giorgio, additional, Grazia, Arena Maria, additional, Parise, Pasquale, additional, Boccali, Andrea, additional, Amici, Serena, additional, Gambacorta, Maurizia, additional, Lasagni, Anna, additional, Lovati, Roberto, additional, Giovinazzo, Francesca, additional, Kimak, Elzbieta, additional, Zappa, Paolo, additional, Medici, Francesco, additional, Lo Castro, Marika, additional, Mauro, Flavia, additional, De Luca, Alessandro, additional, Sancesario, Giuseppe, additional, Martorana, Alessandro, additional, Scaricamazza, Beatrice, additional, Toniolo, Sofia, additional, Di Lorenzo, Francesco, additional, Liguori, Claudio, additional, Lasco, Antonino, additional, Vita, Natale, additional, Giomi, Mirna, additional, Forte, Floriana, additional, Padovani, Alessandro, additional, Rozzini, Luca, additional, Ceraso, Anna, additional, Salvatore, Caratozzolo, additional, Cottino, Maria, additional, Vitali, Silvia, additional, Marelli, Eleonora, additional, Tripi, Gabriele, additional, Miceli, Salvatore, additional, Urso, Giovanni, additional, Grioni, Giuseppe, additional, Vezzadini, Giuliana, additional, Misaggi, Giulia, additional, Forlani, Chiara, additional, Avanzi, Stefano, additional, Serena, Saulle, additional, Claudia, Casarini, additional, Marilena, Viggiano, additional, Alberto, Luisa, additional, Diego, Ghianda, additional, Alessandro, Giordano, additional, Iemolo, Francesco, additional, Giordano, Antonello, additional, Sanzaro, Enzo, additional, D'Asta, Gabriele, additional, Proietto, Maria, additional, Carnemolla, Anna, additional, Razza, Grazia, additional, Spadaro, Daniela, additional, Bertolotti, Marco, additional, Mussi, Chiara, additional, Neviani, Francesca, additional, Roberto, Chiesa, additional, Valentina, Guerzoni, additional, Linda, Morselli, additional, Francesca, Venturelli, additional, Tarozzi, Adriano, additional, Balestri, Francesca, additional, Mannarino, Giulio, additional, Bigolari, Michela, additional, Natale, Alessia, additional, Grassi, Simona, additional, Bottaro, Cinzia, additional, Stefanelli, Sara, additional, Bovone, Ugo, additional, Tortorolo, Umberto, additional, Quadri, Roberto, additional, Leone, Giuseppe, additional, Ponzetto, Maria, additional, Frasson, Paola, additional, Annoni, Giorgio, additional, Bruni, Adriana, additional, Confalonieri, Roberto, additional, Corsi, Maurizio, additional, Moretti, Daniela, additional, Teruzzi, Fabiola, additional, Umidi, Simona, additional, Mazzola, Paolo, additional, Perego, Sabrina, additional, Persico, Ilaria, additional, Olivieri, Giulia, additional, Bonfanti, Alessandra, additional, Hajnalka, Szabò, additional, Galeazzi, Marianna, additional, Massariello, Francesca, additional, Anzuini, Alessandra, additional, Caffarra, Paolo, additional, Barocco, Federica, additional, Spallazzi, Marco, additional, Paolo, Ceda Gian, additional, Simonetta, Morganti, additional, Andrea, Artoni, additional, Chioatto, Paolo, additional, Bortolamei, Sandra, additional, Soattin, Lucia, additional, Ruotolo, Giovanni, additional, Beneamino, Borelli, additional, Giuseppe, Barilaro, additional, Bertazzoli, Marco, additional, Rota, Elisabetta, additional, Adobati, Annamaria, additional, Scarpa, Alberto, additional, Granziera, Serena, additional, Zuccher, Paola, additional, Fabbro, Angela Dal, additional, Zara, Daniela, additional, Lo Nigro, Ambra, additional, Franchetti, Lorena, additional, Toniolo, Marika, additional, Marcuzzo, Cinzia, additional, Rollone, Marco, additional, Guerriero, Fabio, additional, Sgarlata, Carmelo, additional, Massè, Alessandro, additional, Zatti, Giovanni, additional, Piatti, Massimiliano, additional, Graci, Jole, additional, Benati, Giuseppe, additional, Boschi, Federica, additional, Biondi, Mario, additional, Fiumi, Nicoletta, additional, Erika, Tamburini, additional, Locatelli, Sergio Mario, additional, Mauri, Sabrina, additional, Beretta, Mauro, additional, Margheritis, Laura, additional, Desideri, Giovanbattista, additional, Liberatore, Ester, additional, Carucci, Anna Cecilia, additional, Bonino, Paolo, additional, Caput, Margherita, additional, Antonietti, Maria Paola, additional, Polistena, Giuseppe, additional, De la Pierre, Franz, additional, Mari, Marcello, additional, Massignani, Paola, additional, Tombesi, Fabio, additional, Selvaggio, Fabio, additional, Verbo, Brunella, additional, Bodoni, Paolo, additional, Marchionni, Niccolò, additional, Sabatini, Tony, additional, Mussio, Eleonora, additional, Magni, Eugenio, additional, Bianchetti, Angelo, additional, Crucitti, Andrea, additional, Titoldini, Giulia, additional, Cossu, Beatrice, additional, Fascendini, Sara, additional, Licini, Cristina, additional, Tomasoni, Angela, additional, Calderazzo, Massimo, additional, Daniela, Tropiano, additional, Valentina, Laganà, additional, Melotti, Rita Maria, additional, Lilli, Albina, additional, Buda, Simona, additional, Adversi, Marco, additional, Noro, Gabriele, additional, Turco, Renato, additional, Ubezio, M Chiara, additional, Mantovani, Anna Roberta, additional, Viola, Maria Cristina, additional, Serrati, Carlo, additional, Pretta, Stefano, additional, Infante, Maria, additional, Gentile, Simona, additional, Morandi, Alessandro, additional, D'Ambrosio, Viviana, additional, Mazzanti, Paolo, additional, Brambilla, Cristina, additional, Sportelli, Silvia, additional, Platto, Caterina, additional, Faraci, Bianca, additional, Quattrocchi, Daniela, additional, Pernigotti, Luigi Maria, additional, Pisu, Cristina, additional, Sicuro, Francesca, additional, Zagnoni, Piergiuseppe, additional, Ghiglia, Stefania, additional, Mosca, Massimiliano, additional, Corazzin, Ileana, additional, Deola, Mariangela, additional, Biagini, Carlo Adriano, additional, Bencini, Francesca, additional, Cantini, Claudia, additional, Tonon, Elisabetta, additional, Pierinelli, Silvia, additional, Onofrj, Marco, additional, Thomas, Astrid, additional, Filomena, Borbone, additional, Bonanni, Laura, additional, Gabriella, Cacchiò, additional, Comi, Giancarlo, additional, Magnani, Giuseppe, additional, Santangelo, Roberto, additional, Mazzeo, Salvatore, additional, Giuseppe, Magnani, additional, Francesca, Caso, additional, Giordano, Cecchetti, additional, Roberto, Sant'Angelo, additional, Barbieri, Cristina, additional, Giroldi, Liviana, additional, Bandini, Fabio, additional, Masina, Marco, additional, Malservisi, Simona, additional, Cicognani, Annalena, additional, Ricca, Laura, additional, Piccininni, Maristella, additional, Tassinari, Tiziana, additional, Brogi, Davide, additional, Sugo, Annalisa, additional, Alessandra, Filippi, additional, Sonia, Manfredi, additional, Valerio, Vanni, additional, Andrea, Usai Carlo, additional, Enrico, Colombo, additional, Vera, Renna Francesca, additional, Assunta, Sauchella, additional, Gianmaria, Zavarise, additional, Mauro, Prete, additional, Pietro, Bisagni, additional, Roberto, Masini, additional, Salvatore, Carrabetta, additional, Barone, Antonella, additional, Razzano, Monica, additional, Giuseppe, Imperoli, additional, Angela, Bini, additional, Francesco, Serra, additional, Valeria, D'Agostino, additional, Federico, Gianolla, additional, Lucia, Pietrangeli, additional, Antonella, Velardi, additional, Elisabetta, Di Cello, additional, Cristina, Rosati, additional, Nadia, Casali, additional, Maria, Sessa, additional, Luciano, Abruzzi, additional, Chiara, Costanzi, additional, Bini, Paolo, additional, Pignata, Michele, additional, Enrico, Buonagurio, additional, Maria, Vollery, additional, Giovanni, Carrieri, additional, Giorgio, Cioni, additional, Andrea, Toschi, additional, Marco, Metra, additional, Piera, Ranieri, additional, Alberto, Zucchelli, additional, Ceccon, Anna, additional, Magrin, Laura, additional, Marin, Sara, additional, Barbara, Sofia, additional, Marco, Masina, additional, Laura, Ghedini, additional, Matteo, Moroni, additional, Marco, Paragona, additional, Caterina, Pallotti Maria, additional, Carla, Rota, additional, Federica, Gottardi, additional, Clara, Tomasoni, additional, Melania, Cappuccio, additional, Giampaolo, Bonini, additional, Stefano, Guerini, additional, Valeria, Guerini, additional, Lucia, Merla, additional, Giovambattista, Desideri, additional, Ester, Liberatore, additional, Cecilia, Carucci Anna, additional, Maurizio, Tovaglieri, additional, Vera, Renna Frandesca, additional, Nadia, Bongiorni, additional, Grillo, Antonio, additional, Arenare, Francesca, additional, Tonino, Mineo, additional, David, Kanah, additional, Giorgio, Vianello Piero, additional, Ubaldo, Balducci, additional, Vincenzo, Sidoti, additional, Stefano, Montanari, additional, Marino, Formilan, additional, Busonera Flavio, Murelli Teodolindo, additional, Paolo, Albanese, additional, Monica, Maselli, additional, Francesco, Bolzetta, additional, Roberto, Fabris, additional, Paolo, Bonino, additional, DuranteMangoni, Emanuele, additional, Testoni, Michela, additional, Fabio, Di Stefano, additional, Loredana, Seccia, additional, Valeria, Sonzini, additional, Fabiano, Maria, additional, Annabella, Di Giorgio, additional, Salvatore, De Cosmo, additional, Greco, Antonio, additional, Grazia, D'Onofrio, additional, Daniele, Sancarlo, additional, Gianluca, Resta, additional, Renzo, Girardello, additional, Sergio, Minervini, additional, Morena, Boni, additional, Vitali, Mariagrazia, additional, Marina, Pizzoni, additional, Paolo, De Colle, additional, Cristina, Scarpa, additional, Alessandra, Frattola, additional, Orlandini, Francesco, additional, La Regina, Micaela, additional, Desirée, Addesi, additional, Mirella, Filippo, additional, Mario, Bo, additional, Paola, Porrino, additional, Padulo, Filomena, additional, Cristina, Margheriti, additional, Dario, Rolano, additional, Giancarla, Moscatelli, additional, Guido, Radaelli, additional, Elena, Montini, additional, Prete, Cosimo, additional, Marileda, Novello, additional, Igor, Bramuzzo, additional, Nicole, Bertin, additional, Elena, Rinaldo, additional, Paolillo, Ciro, additional, Riccardi, Angela, additional, Claudia, Benedetti, additional, Barbara, Rizzi, additional, Francesca, Montagna, additional, Silvia, Vitali, additional, Chiara, Cutaia, additional, Oliver, Baca, additional, Mauro, Colombo, additional, Eleonora, Marelli, additional, Giuseppe, Procino, additional, Rosaria, Tararà, additional, Maria, Cottino, additional, Davide, Dell'Acqua, additional, Stefania, Cislaghi, additional, Marco, Cairati, additional, Massimo, Porta, additional, Luca, Scaglione, additional, Martina, De Feo, additional, Paola, Vertolli, additional, Lia, Salvati, additional, Sandro, Cinotti, additional, Valentina, De Santis, additional, Erminia, Biferi, additional, Paola, Cheli, additional, Romina, Rebizzo, additional, Minisola, Salvatore, additional, Luciano, Colangelo, additional, Pasquale, Abete, additional, Ilaria, Liguori, additional, Francesca, Curcio, additional, Guglielmo, Spassini, additional, Marco, Engheben, additional, Sara, Rotunno, additional, Paola, Arosio, additional, Claudio, Angelini, additional, Francesco, Reggiani, additional, Alessandro, Cappelli, additional, Simona, Marcheselli, additional, Lara, Fratticci, additional, Paola, Ranzani, additional, Simonetta, Cesarini, additional, Antonella, Cerini, additional, Generoso, Uomo, additional, Fernando, Gallucci, additional, Giuliano, Ceschia, additional, Emanuela, Serra, additional, Mariolina, Sola, additional, Alessandro, Delitala, additional, Chiara, Pes, additional, Giulia, Lobianco, additional, Alessandro, Giani, additional, Famularo, Simone, additional, Sandini, Marta, additional, Pinotti, Enrico, additional, Gianotti, Luca, additional, Antonella, Battuello, additional, Giulia, Pintore, additional, Sante, Giardini, additional, Rossi, Andrea, additional, Rubele, Sofia, additional, Sant, Selena, additional, Marco, Vignati, additional, Danila, Clerici, additional, Fabio, Rosa, additional, Bandirali, Maria Pia, additional, Nicoletta, Cattaneo, additional, Laura, Boffi, additional, Paolo, Tosoni, additional, Luciano, Terranova, additional, Leonello, Avalli, additional, Margherita, Scanziani, additional, Stefania, De Notaris, additional, Pierluigi, Del Santo, additional, Laura, Rossi, additional, Fabiana, Tezza, additional, Giovanna, Cervati, additional, Antonino, Andreolli, additional, Felice, Catalano, additional, Giuseppe, Burattin, additional, Danilo, Fogli, additional, Giovanna, Di Bella, additional, Francesco, Landi, additional, Salini, Sara, additional, Angela, Brunetti Maria, additional, Giorgetta, Cappa, additional, Giovanni, Galvagno, additional, Paola, Cena, additional, Gerardo, Bruno, additional, Silvio, Raspo, additional, Letizia, Semeraro, additional, Sabrina, Pedrotti, additional, Davide, Barra, additional, Rosaria, Rizzo Maria, additional, Maria, Dalise Anna, additional, Raffaele, Prestano, additional, Palmieri, Vincenzo Ostilio, additional, Palasciano, Giuseppe, additional, Belfiore, Anna, additional, Portincasa, Piero, additional, Carlo, Sabbà, additional, Vincenzo, Solfrizzi, additional, Alessia, D'introno, additional, Valiani, Vincenzo, additional, Carolina, Bologna, additional, Tiziana, Ciarambino, additional, Paola, Turelli, additional, Ugo, Pazzaglia, additional, Federica, Rodella, additional, Giacomo, Piana, additional, Castellano, Maurizio, additional, Anna, Garelli, additional, Elisa, Casella, additional, Federica, Campana, additional, Antonietta, Coschignano Maria, additional, Luigi, Marinangeli, additional, Fabio, Lorico, additional, Salvatore, Bazzano, additional, Giuseppe, Menculini, additional, Gelosa, Giorgio, additional, Viviana, Ambrogio Teresa, additional, Piras, Valentina, additional, Andrea, Ciricugno, additional, Alessandra, Bollari, additional, Coen, Daniele, additional, Magliola, Renata, additional, Milanesio, Deborah, additional, Muzzulini, Carlo Lorenzo, additional, Paolo, Fogliacco, additional, Marinella, Turla, additional, Sofia, Cotelli Maria, additional, Marta, Bianchi, additional, Siano, Pietro, additional, Capo, Giuseppe, additional, Napoletano, Rosa, additional, Cecilia, Politi, additional, Mancini, Concetta, additional, Del Buono, Corrado, additional, De Bartolomeo, Giuseppe, additional, Addolorata, Martinelli, additional, Carmen, Cefalogli, additional, Roberto, Cozzi, additional, Giovanni, Virtuani Angelo, additional, Moschettini, Gianfilippo, additional, Franco, Mastroianni, additional, Daniela, Roglia, additional, D'Amico, Gabriella, additional, Mirella, Palella, additional, Endrizzi, Cristina, additional, Trotta, Lucia, additional, Ciarambino, Tiziana, additional, Orazio, Zanetti, additional, Emanuela, Terazzi, additional, Marta, Sacchetti, additional, Thomas, Fleetwood, additional, Giacomo, Tondo, additional, Ignazio, Di Fazio, additional, Andrea, Bruni, additional, Giuseppe, Orsitto, additional, Emanuela, Fabbro, additional, Serena, Amici, additional, Elena, D'Imporzano, additional, Anna, Casanova, additional, Serena, Bertolio, additional, Erika, Nervo, additional, Roberto, Silvestri, additional, Elena, Semproni, additional, Manuela, Pintus, additional, Francesca, Aloe, additional, and Angelo, Tagliaccica, additional
Published: 2020
Full Text: View/download PDF

24. Evaluation of trait and state anxiety levels in a group of peri- and postmenopausal women

Author: Margus Salinas, Mónica Flores-Ramos, José Benjamín Guerrero-López, and Roberto Silvestri Tomassoni
Subjects: Adult, medicine.medical_specialty, Peri, Anxiety, 03 medical and health sciences, 0302 clinical medicine, Surveys and Questionnaires, medicine, Humans, Aged, 030219 obstetrics & reproductive medicine, Postmenopausal women, Obstetrics, General Medicine, Middle Aged, Perimenopause, Postmenopause, Cross-Sectional Studies, Hot Flashes, Trait, Hormonal therapy, Female, medicine.symptom, Psychology, 030217 neurology & neurosurgery, Clinical psychology
Abstract: Our objective was to evaluate levels of trait and state anxiety in a group of peri- and postmenopausal women and to explore the relation of hormonal therapy to levels of anxiety. Peri- (n = 63) and postmenopausal (n = 236) women were evaluated between March and September 2013. The assessed variables were menopausal status, anxiety (using the state and trait anxiety inventory), and sociodemographic and clinical variables. Use of psychotropic medications and hormone therapy was also ascertained. The mean age of the participants was 51.9 years, ranging from 31 to 69 years. The mean state anxiety scores, as well as the mean trait anxiety scores, were higher in perimenopausal than postmenopausal women. High state anxiety (above the 75th percentile), but not high trait anxiety, was related to perimenopausal status. Anxiety levels appeared to be higher among perimenopausal than postmenopausal women, as also occurs with depressive symptoms. Anxiety state provides data about recent anxiety symptoms in women; however, anxiety trait could be present in some women before perimenopause. Our findings suggest that perimenopause is a period with increased anxiety levels in some women.
Published: 2017
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25. T‐type calcium channels drive the proliferation of androgen‐receptor negative prostate cancer cells

Author: Sushilaben H. Rigas, Romina Aceto, Katja Rietdorf, Chrysanthi Matheou, Martin D. Bootman, Francesco Crea, Yuzhuo Wang, Perla Pucci, Stephen Chandler, Erik Venalainen, Rebecca L. Mather, and Roberto Silvestri
Subjects: Male, 0301 basic medicine, Small interfering RNA, Urology, urologic and male genital diseases, Androgen deprivation therapy, Calcium Channels, T-Type, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, Downregulation and upregulation, Cell Line, Tumor, Humans, Medicine, MTT assay, Molecular Targeted Therapy, Cell Proliferation, Cell growth, business.industry, T-type calcium channel, Prostatic Neoplasms, Calcium Channel Blockers, Prognosis, medicine.disease, Up-Regulation, Androgen receptor, 030104 developmental biology, Oncology, Receptors, Androgen, Mibefradil, 030220 oncology & carcinogenesis, PC-3 Cells, Cancer research, Ethosuximide, business
Abstract: Background: Androgen deprivation therapy (ADT) is the treatment of choice for metastatic prostate cancer (PCa). After an initial response to ADT, PCa cells can generate castration resistant (CRPC) or neuroendocrine (NEPC) malignancies, which are incurable. T-type calcium channels (TTCCs) are emerging as promising therapeutic targets for several cancers, but their role in PCa progression has never been investigated. Methods: To examine the role of TTCCs in PCa, we analyzed their expression level, copy number variants (CNV) and prognostic significance using clinical datasets (Oncomine and cBioPortal). We then evaluated TTCC expression in a panel of PCa cell lines and measured the effect of their inhibition on cell proliferation and survival using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and caspase assays. Results: TTCCs were upregulated in PCas harboring androgen receptor (AR) mutations; CNV rate was positively associated with PCa progression. Higher expression of one TTCC isoform (CACNA1G) predicted poorer postoperative prognosis in early stage PCa samples. Pharmacological or small interfering RNA (siRNA)-based inhibition of TTCCs caused a decrease in PC-3 cell survival and proliferation. Conclusions: Our results show that TTCCs are overexpressed in advanced forms of PCa and correlate with a poorer prognosis. TTCC inhibition reduces cell proliferation and survival, suggesting that there may be possible value in the therapeutic targeting of TTCCs in advanced PCa.
Published: 2019
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26. DNA polymerases in the risk and prognosis of colorectal and pancreatic cancers

Author: Stefano Landi and Roberto Silvestri
Subjects: DNA Replication, DNA Repair, DNA damage, DNA polymerase, Health, Toxicology and Mutagenesis, DNA-Directed DNA Polymerase, Toxicology, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Genetics, medicine, Animals, Humans, Genetics (clinical), 030304 developmental biology, 0303 health sciences, Mutation, biology, DNA replication, Cancer, medicine.disease, Prognosis, Pancreatic Neoplasms, chemistry, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Proofreading, Carcinogenesis, Colorectal Neoplasms, DNA, DNA Damage
Abstract: Human cancers arise from the alteration of genes involved in important pathways that mainly affect cell growth and proliferation. DNA replication and DNA damages recognition and repair are among these pathways and DNA polymerases that take part in these processes are frequently involved in cancer onset and progression. For example, damaging alterations within the proofreading domain of replicative polymerases, often reported in patients affected by colorectal cancer (CRC), are considered risk factors and drivers of carcinogenesis as they can lead to the accumulation of several mutations throughout the genome. Thus, replicative polymerases can be involved in cancer when losses of their physiological functions occur. On the contrary, reparative polymerases are often involved in cancer precisely because of their physiological role. In fact, their ability to repair and bypass DNA damages, which confers genome stability, can also counteract the effect of most anticancer drugs. In addition, the altered expression can characterise some type of cancers, which exacerbates this aspect. For example, all of the DNA polymerases involved a damage bypass mechanism, known as translesion synthesis, with the only exception of polymerase theta, are downregulated in CRC. Conversely, in pancreatic ductal adenocarcinoma (PDAC), most of these polymerase result upregulated. This suggests that different types of cancer can rely on different reparative polymerases to acquire drug resistance. Here we will examine all of the aspects that link DNA polymerases with CRC and PDAC.
Published: 2019

27. The winning shares in the major football leagues

Author: Roberto Silvestri, Riccardo Izzo, Felice Di Domenico, and Tiziana D'Isanto
Subjects: Ball possession, Physical activity, Advertising, Free space, Football, Soccer, Match analysis, Video analysis, Shot, League, EPIC, Political science, Educación Física y Deportiva, Technical skills
Abstract: The new millennium was marked by the epic of the legendary Barcelona of Guardiola, the coach who revolutionized the world of football with his tiki taka. The search for this game made of fast ball exchanges, continuous changes of position and search for free space to occupy, however, has downgraded what is one of the main technical skills of the game of football, the dribbling. Starting with football schools Pedagogical value of the body and physical activity in childhood. 1vs1 has been set aside to favour other technical fundamentals, so we are in a period where fewer and fewer players take responsibility for trying a game in 1vs1, but prefer to pass the ball to their closest partner. This second study has analysed the scorer rankings of the top 4 European championships (Serie A, Premier League, Spanish League, Bundesliga) of the 2009/2010 and 2017/2018 seasons, taking into account not only the total goals, but also the average shots per game and the average dribbling per game.
Published: 2019

28. Italia e Portogallo: rencontre unique

Author: Roberto Silvestri
Published: 2016
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29. Recreational sea fishing in Europe in a global contextParticipation rates, fishing effort, expenditure, and implications for monitoring and assessment

Author: Janis Birzaks, Tomas Zolubas, Hans Jakob Olesen, Anders Kagervall, Graham G. Monkman, Alf Ring Kleiven, Anssi Ahvonen, David Turnbull, Jon Ruiz, Harry Vincent Strehlow, Ryszard Grzebielec, Roi Martinez, Jeremy M. Lyle, Bryony Townhill, Giulia Cambiè, Trude Borch, Pablo Pita, Thomas Verleye, Paul O'Reilly, Jon-Helge Vølstad, Estanis Mugerza, Arvydas Svagzdys, Tessa van der Hammen, Zachary Radford, Adam M Lejk, Roberto Silvestri, Martin de Graaf, Kieran Hyder, Ana Gordoa, Scott R. Steinback, William Roche, Delphine Rocklin, Beatriz Morales-Nin, Martin Karlsson, Lucia Zarauz, Andreas Sundelöf, Bruce Hartill, Manuel Bellanger, Kostas Kapiris, Callum Scougal, Marc Simon Weltersbach, Pedro Veiga, Łukasz Dziemian, Pentti Moilanen, Harold Levrel, Frankwin van Winsen, Mike Armstrong, Christian Skov, Robert Arlinghaus, Sabrina Lovell, Andrei Baikov, Anastasios Papadopoulos, Hugo Diogo, Krzysztof Radtke, David van Voorhees, Keno Ferter, Centre for Environment, Fisheries and Aquaculture Science [Lowestoft] (CEFAS), Institute of Baltic Sea Fishery (OSF), Institute of Marine Research [Bergen] (IMR), University of Bergen (UiB), Natural Resources Institute Finland (LUKE), Humboldt-Universität zu Berlin, Leibniz-Institut für Gewässerökologie und Binnenfischerei (IGB), Leibniz Association, University of Tartu, Aménagement des Usages des Ressources et des Espaces marins et littoraux - Centre de droit et d'économie de la mer (AMURE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Akvaplan-Niva [Tromsø], Norwegian Institute for Water Research (NIVA), Wageningen University and Research [Wageningen] (WUR), Department of Oceanography and Fisheries [Horta], University of the Azores, National Marine Fisheries Research Institute, Centre d'Estudis Avançats de Blanes (CEAB), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), National Institute of Water and Atmospheric Research [Auckland] (NIWA), Swedish University of Agricultural Sciences (SLU), Hellenic Centre for Marine Research (HCMR), centre international de recherche sur l'environnement et le développement (CIRED), Centre National de la Recherche Scientifique (CNRS)-École des Ponts ParisTech (ENPC)-École des hautes études en sciences sociales (EHESS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), NOAA National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), Institute for Marine and Antarctic Studies [Horbat] (IMAS), University of Tasmania [Hobart, Australia] (UTAS), School of Ocean Sciences [Menai Bridge], Bangor University, Institut Mediterrani d'Estudis Avancats (IMEDEA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Universidad de las Islas Baleares (UIB), AZTI - Tecnalia, Inland Fisheries Ireland, DTU Aqua, National Institute of Aquatic Resources, Technical University of Denmark [Lyngby] (DTU), Hellenic Center for Marine Research (HCMR), Universidade de Santiago de Compostela [Spain] (USC ), Memorial University of Newfoundland [St. John's], Centro Interuniversitario di Biologia Marina ed Ecologia Applicata (C.I.B.M.), Northeast Fisheries Science Center (NEFSC), National Oceanic and Atmospheric Administration (NOAA)-National Oceanic and Atmospheric Administration (NOAA), Marine Scotland, Research Institute for Agricultural, Fisheries and Food (ILVO), Vlaams Instituut voor de Zee, Centre of Marine Sciences [Faro] (CCMAR), University of Algarve [Portugal], Humboldt University Of Berlin, Institute of Food Safety, Animal Health and Environment (BIOR), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École des hautes études en sciences sociales (EHESS)-AgroParisTech-École des Ponts ParisTech (ENPC)-Centre National de la Recherche Scientifique (CNRS), Institute for Marine and Antarctic Studies [Hobart] (IMAS), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), and Memorial University of Newfoundland = Université Memorial de Terre-Neuve [St. John's, Canada] (MUN)
Subjects: 0106 biological sciences, Oceanography, Fish stock, 01 natural sciences, Gadus, Economic value, Cod Gadus-Morhua, Fisheries Management, Surveys and monitoring of marine recreational fisheries, media_common, 2. Zero hunger, biology, Participation, 04 agricultural and veterinary sciences, Geography, Social benefits, Impact, [SDE]Environmental Sciences, Industrialized Countries, Fisheries management, Fishing, Context (language use), United-States, Conservation, Management, Monitoring, Policy and Law, Aquatic Science, Onderzoeksformatie, Atlantic Cod, European marine recreational fisheries, media_common.cataloged_instance, 14. Life underwater, SDG 14 - Life Below Water, European union, Recreation, Ecology, Evolution, Behavior and Systematics, Marine, 010604 marine biology & hydrobiology, Gadidae, Semi‐quantitative bias assessment, Sustainable fisheries management, biology.organism_classification, Fish stocks, Fishery, [SDU]Sciences of the Universe [physics], Marine recreational fishing, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Fishing effort and expenditure, Fisheries assessment and management, Catch
Abstract: Este artículo contiene 19 páginas, 7 tablas, 4 figuras., Marine recreational fishing (MRF) is a high-participation activity with large economic value and social benefits globally, and it impacts on some fish stocks. Although reporting MRF catches is a European Union legislative requirement, estimates are only available for some countries. Here, data on numbers of fishers, participation rates, days fished, expenditures, and catches of two widely targeted species were synthesized to provide European estimates of MRF and placed in the global context. Uncertainty assessment was not possible due to incomplete knowledge of error distributions; instead, a semi-quantitative bias assessment was made. There were an estimated 8.7 million European recreational sea fishers corresponding to a participation rate of 1.6%. An estimated 77.6 million days were fished, and expenditure was €5.9 billion annually. There were higher participation, numbers of fishers, days fished and expenditure in the Atlantic than the Mediterranean, but the Mediterranean estimates were generally less robust. Comparisons with other regions showed that European MRF participation rates and expenditure were in the mid-range, with higher participation in Oceania and the United States, higher expenditure in the United States, and lower participation and expenditure in South America and Africa. For both northern European sea bass (Dicentrarchus labrax, Moronidae) and western Baltic cod (Gadus morhua, Gadidae) stocks, MRF represented 27% of the total removals. This study highlights the importance of MRF and the need for bespoke, regular and statistically sound data collection to underpin European fisheries management. Solutions are proposed for future MRF data collection in Europe and other regions to support sustainable fisheries management., Institut Français de Recherche pour l’Exploitation de la Mer; French Ministry of Fisheries Management; Greek National Data Collection Programme; European Commission, Data Collection Framework; Department for Environment, Food and Rural Affairs, Grant/Award Number: MF1221, MF1230 and MI001; Norges Forskningsråd, Grant/Award Number: A framework for science-based management of marine recreational fisheries in Norway (267808); State Department of Agriculture, Food Security and Fisheries Mecklenburg- Western Pomerania; Interreg IVa 2 Seas, Grant/Award Number: GIFS project; Dutch Ministry of Economic Affairs, Grant/Award Number: WOT (Wettelijke Onderzoekstaken) programme; European Fishery Fund - Axis 4, approved by FLAG; Department of Environment, Planning, Agriculture and Fisheries from the Basque Government; Government of Galicia, Grant/Award Number: RECREGES project (ED481B2014/034-0)
Published: 2018
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30. Bambole perverse

Author: Mariuccia Ciotta, Roberto Silvestri, Mariuccia Ciotta, and Roberto Silvestri
Abstract: Che Diva sei? È la domanda per ‘indossare'la propria maschera, e in ogni sembianza, sotto ogni copertura, ridisegnare i generi o fluttuarvi delicatamente da un lato all'altro. Dive e Divine insegnano a smantellare i ‘tipi'di donne (e di uomini), divise in categorie, classificate a seconda del fascino e della bellezza, ma come scrive Francis Scott Fitzgerald: ‘Lui voleva fare di me una Dea, invece io voglio essere Mickey Mouse'.” Un “catalogo delle dive” cinematografiche che racconta le donne del grande schermo, dalle icone intramontabili del passato alle loro eredi di oggi, facendo saltare convenzioni e generi, sgretolando gli stereotipi. Da Mary Pickford a Emma Watson, da Clara Bow a Meg Ryan, da Katharine Hepburn a Kristen Stewart, da Glenn Close a Tilda Swinton: donne libere ed eversive, che frantumano le mode imponendone di nuove, scompigliano la scena e il bon ton borghese creando categorie inedite e rivoluzionarie. Donne inimitabili, bellissime, che siano “maschiette”, icone del femminismo o dive dal fascino noir, tutte rifiutano le etichette più banali per imporre la loro identità.
Published: 2018

31. 2. International Film Criticism Today: A Critical Symposium

Author: Jorge Jellinek, Adrian Martin, Meenakshi Shedde, Christoph Huber, Li Cheuk-to, Leonardo García Tsao, Roberto Silvestri, Eduardo Antin, Tadao Sato, Pedro Butcher, Jonathan Romney, Leon van Nierop, Jean-Michel Frodon, Olaf Möller, Michel Ciment, Noel Vera, Tullio Kezich, Li Hongyu, Tahar Chikhaoui, Angelike Contis, Lev Karakhan, and Kong Rithdee
Subjects: Political science, Media studies, Criticism
Published: 2017
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32. Mesothelin promoter variants are associated with increased soluble mesothelin-related peptide levels in asbestos-exposed individuals

Author: Roberto Silvestri, Stefano Landi, Ombretta Melaiu, Monica Cipollini, Irene Lepori, Irene Dell’Anno, Chiara De Santi, Elisa Paolicchi, Alfonso Cristaudo, Perla Pucci, Alessandra Bonotti, Lucia Pellè, Federica Gemignani, Pavel Vodicka, Veronika Vymetalkova, Luciano Mutti, Elisa Barone, Alda Corrado, and Rudy Foddis
Subjects: 0301 basic medicine, Male, Mesothelioma, Linkage disequilibrium, health surveillance, Lung Neoplasms, Genotype, In silico, Single-nucleotide polymorphism, GPI-Linked Proteins, Settore MED/05, Polymorphism, Single Nucleotide, Sensitivity and Specificity, mesothelioma, polymorphisms, Aged, Alleles, Analysis of Variance, Asbestos, Biomarkers, Tumor, Female, Humans, Italy, Luciferases, Middle Aged, Public Health, Environmental and Occupational Health, 03 medical and health sciences, 0302 clinical medicine, SNP, Mesothelin, Allele, Polymorphism, Genetics, Tumor, biology, Haplotype, Mesothelioma, Malignant, Environmental and Occupational Health, Single Nucleotide, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, Public Health, Biomarkers
Abstract: BACKGROUND:\ud Soluble mesothelin-related peptide (SMRP) is a promising diagnostic biomarker for malignant pleural mesothelioma (MPM), but various confounders hinder its usefulness in surveillance programmes. We previously showed that a single nucleotide polymorphism (SNP) within the 3'untranslated region (3'UTR) of the mesothelin (MSLN) gene could affect the levels of SMRP.\ud OBJECTIVES:\ud To focus on SNPs located within MSLN promoter as possible critical genetic variables in determining SMRP levels.\ud METHODS:\ud The association between SMRP and SNPs was tested in 689 non-MPM subjects and 70 patients with MPM. Reporter plasmids carrying the four most common haplotypes were compared in a dual luciferase assay, and in silico analyses were performed to investigate the putative biological role of the SNPs.\ud RESULTS:\ud We found a strong association between serum SMRP and variant alleles of rs3764247, rs3764246 (in strong linkage disequilibrium with rs2235504) and rs2235503 in non-MPM subjects. Inclusion of the genotype information led to an increase in SMRP specificity from 79.9% to 85.5%. Although not statistically significant, the group with MPM showed the same trend of association. According to the in vitro luciferase study, rs3764247 itself had a functional role. In silico approaches showed that the binding sites for transcription factors such as Staf and ZNF143 could be affected by this SNP. The other SNPs were shown to interact with each other in a more complex way.\ud CONCLUSIONS:\ud These data support the suggestion that SMRP performance is affected by individual (ie, genetic) variables and that MSLN expression is influenced by SNPs located within the promoter regulatory region.
Published: 2017

33. A Common Polymorphism Within MSLN Affects miR-611 Binding Site and Soluble Mesothelin Levels in Healthy People

Author: Sonia Garritano, Marco Lucchi, Federica Gemignani, Luciano Mutti, Alfonso Cristaudo, Rudy Foddis, Ombretta Melaiu, Roberto Silvestri, Alessandra Bonotti, Roberto Barale, Stefano Landi, Chiara De Santi, Monica Cipollini, and Elisa Barone
Subjects: Mesothelioma, Untranslated region, Pulmonary and Respiratory Medicine, Lung Neoplasms, mesothelin, microRNA, Malignant pleural mesothelioma, Biology, GPI-Linked Proteins, Polymorphism, Single Nucleotide, Settore MED/05, Soluble mesothelin, Western blot, Genotype, medicine, Humans, Mesothelin, Binding site, Allele, Gene, related peptide, Binding Sites, Polymorphism, Genetic, medicine.diagnostic_test, Mesothelioma, Malignant, MicroRNA, Transfection, Middle Aged, Molecular biology, miRSNP, Single nucleotide polymorphism, MicroRNAs, Oncology, Case-Control Studies, biology.protein
Abstract: Introduction Soluble mesothelin related peptide (SMRP) was proposed as a promising diagnostic marker for malignant pleural mesothelioma (MPM). In a previous study, we found that rs1057147 within the 3′ untranslated region of MSLN gene was associated with SMRP levels. Thus, we aimed to (1) confirm the previous association on a large series of volunteers and (2) test the hypothesis that the SNP could affect microRNA binding sites. Methods The association analysis was verified in 759 subjects. Then, in silico predictions highlighted miR-611 and miR-887 as candidate miRNAs binding to the polymorphic site. Thus, chimeric constructs bearing the alternative alleles (G > A) were assayed alone or in cotransfection with the miRNA mimics, with dual luciferase reporter assay in non-MPM Met-5A cells. The miRNAs were also assayed by western blot analysis for their ability to down-regulate endogenous mesothelin in the MPM Mero-14 cell line. Results We confirmed that, among non-MPM volunteers, GG homozygotes have the lowest SMRP levels. When the genotype is taken into account, the specificity of SMRP as biomarker improves from 79.7% to 85.3%. Dual-luciferase assays showed a significantly lower reporter activity when the vector harbored the G allele as compared to A allele. miR-887 mimic caused a reduced reporter activity of vectors harboring A or G alleles, while miR-611 was effective only on the vector harboring the G allele. Transfection of these miRNAs into Mero-14 cells significantly reduced endogenous MSLN protein. Conclusion SMRP performance as diagnostic biomarker improved by considering the genotype rs1057147. This polymorphism most likely affects a binding site for miR-611.
Published: 2014
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34. Pesca professionale artigianale e pesca ricreativa e sportiva nella costa toscana; possibile una coesistenza pacifica?

Author: Roberto Silvestri, Sabrina Colella, Stefano De Ranieri, and Piero Mannini
Subjects: Conflittualità, Pesca, Costa Toscana
Abstract: Lungo la costa toscana l'attività di pesca professionale più diffusa è quella artigianale, definita anche piccola pesca: circa 470 imbarcazioni con 800 addetti che catturano 1300 tonn annue per un ricavo di oltre 13 milioni di euro, esercitata da imbarcazioni di LFT
Published: 2016

35. Association between depressive symptoms and reproductive variables in a group of perimenopausal women attending a menopause clinic in México City

Author: Roberto Silvestri-Tomassoni, Gerhard Heinze, and Mónica Flores-Ramos
Subjects: Postpartum depression, medicine.medical_specialty, Depression, business.industry, Hormonal replacement therapy, Obstetrics and Gynecology, Middle Aged, medicine.disease, Ambulatory Care Facilities, Menopause, Psychiatry and Mental health, Premenopause, Mexico city, medicine, Humans, Female, Psychiatry, business, Association (psychology), Mexico, Premenstrual dysphoric disorder, Depressive symptoms, Depression (differential diagnoses)
Abstract: The aim of this study was to explore the association between depressive symptoms and some variables related to the reproductive life, such as history of premenstrual dysphoric disorder, antecedent of postpartum depression, previous use of hormonal contraceptives, and current hot flushes, in a group of perimenopausal women attending a menopause clinic. Perimenopausal women, 45 to 55 years old, who had not received hormonal replacement therapy and/or psychotropic medication, were invited to participate in this study. 141 perimenopausal women were included; we obtained their psychiatric and gynecological data, and we evaluated their depressive symptomatology using the CES-D scale. There were a significantly higher number of cases of previous depressive episodes, PMDD and PPD history in depressed patients compared with non-depressed women; current hot flushes prevalence was similar between depressed and non-depressed women. Patients with a PMDD history were more likely to have experienced previous depressive episodes, a PPD history, and high levels of depression. Variables associated with the level of depression were a previous history of PMDD, current hot flushes, and previous depressive episodes. The occurrence of perimenopausal depression is related to a previous history of PMDD, PPD, and depressive episodes; hot flushes only increase the severity of the depressive episode.
Published: 2009
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36. Multiple measurement techniques in a complex satellite antenna integration scenario

Author: Sebastian Schwarz, Gabriele Cosentino, Marco Cotogni, Fabio Di Giorgio, Mathias von Alberti, Stephen Diel, Roberto Silvestri, and Maurizio Di Serio
Subjects: Beam waveguide antenna, Reconfigurable antenna, Engineering, Ground support equipment, business.industry, Interface (computing), Process (computing), Coordinate-measuring machine, law.invention, law, Laser tracker, Electronic engineering, Antenna (radio), business
Abstract: The integration of a large and deployable radar antenna of an earth observation satellite (Copernicus Sentinel-1A of ESA) is the opportunity to apply different measurement techniques at different stages, to ensure precise, unstressed fixation and proper alignment. This paper proposes an example of the synergic and successful exploitation of techniques like 3D Virtual, to simulate all integration phases and specially evaluate accessibility, DEA coordinate measuring machine, to establish structural parts accurate interface and finally laser tracker and theodolites tools during the integration process, to allow alignment of different antenna elements and the implementation of precise shimming at the satellite interfaces. In addition special mechanical ground support equipment (MGSE) were manufactured and equipped with load cells and dial gauges, used during antenna integration and deployment, to monitor any flight hardware over-stress or set-up distortions.
Published: 2015
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37. Incidence of electrocardiographic abnormalities during treatment with human leukocyte interferon-alfa in patients with chronic hepatitis C but without preexisting cardiovascular disease

Author: Franco Sebastiani, Stefania Magnanimi, Roberto Magnanimi, Furio Colivicchi, and Roberto Silvestri
Subjects: Pharmacology, medicine.medical_specialty, Heart disease, business.industry, Incidence (epidemiology), Alpha interferon, Right bundle branch block, medicine.disease, Gastroenterology, Surgery, Tolerability, Internal medicine, Cohort, medicine, Pharmacology (medical), business, Adverse effect, Interferon alfa, medicine.drug
Abstract: In several clinical settings, interferons (IFNs) have been associated with an increased incidence of cardiovascular side effects and adverse reactions. Accordingly, the aim of the present study was to assess the incidence of electrocardiographic abnormalities during long-term therapy with human leukocyte IFN-alfa in a cohort of 89 consecutive patients (51 men and 38 women; mean age, 43.1 ± 11.4 years) affected by chronic hepatitis C but without preexisting cardiovascular disease. All patients included in the study were followed up for 12 months during treatment with 3 million international units of human leukocyte IFN-alfa administered parenterally three times a week. During therapy, no significant side effects or adverse reactions were noted. However, 3 patients developed an intraventricular conduction defect (right bundle branch block in 2 patients and left anterior hemiblock in 1 patient), and 2 patients showed unifocal premature ventricular contractions in repeated electrocardiographic examinations. The low incidence (5 [5.6%] of 89 patients) of nonspecific electrocardiographic abnormalities in our study population during IFN-alfa treatment supports the tolerability and clinical effectiveness of this drug for long-term treatment of patients with chronic hepatitis C.
Published: 1998
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38. Ticlopidine-induced chronic cholestatic hepatitis: A case report

Author: Franco Sebastiani, Stefania Magnanimi, Roberto Silvestri, Roberto Magnanimi, and Furio Colivicchi
Subjects: Pharmacology, medicine.medical_specialty, Endoscopic retrograde cholangiopancreatography, medicine.diagnostic_test, business.industry, medicine.disease, Asymptomatic, Surgery, Discontinuation, Cholestasis, Cholestatic hepatitis, Liver biopsy, medicine, Itching, Pharmacology (medical), medicine.symptom, Ticlopidine, business, medicine.drug
Abstract: We report a case study of a patient with cholestatic hepatitis undergoing treatment with oral ticlopidine. The patient was admitted to our hospital for a transient ischemic attack, and after 40 days of ticlopidine therapy developed cholestatic jaundice, fever, itching, and arthralgias. Results from an ultrasound, computed tomography scan, and endoscopic retrograde cholangiopancreatography eliminated the presence of biliary obstruction; results from a liver biopsy showed a histopathologic picture consistent with drug-induced cholestatic hepatitis. One year after discontinuation of ticlopidine, the patient was still asymptomatic, but laboratory data showed relevant abnormalities, indicating persistence of cholestasis and hepatic injury. This is the first reported instance of a patient with cholestatic hepatitis that developed during treatment with ticlopidine and persisted despite interruption of drug administration.
Published: 1994
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39. Eficacia de la intervención terapéutica en la composición corporal (grasa corporal e índice de masa corporal) en las pacientes con síndrome climatérico, medido por DEXA.

Author: Tomassoni, José Roberto Silvestri and Luna, Guillermo Federico Ortíz
Subjects: *BODY composition, *BODY mass index, *ESTROGEN, *CLIMACTERIC, *WOMEN
Abstract: Objective: To evaluate if therapeutic intervention changes body composition of women with climacteric syndrome. Patients and method: A prospective, longitudinal study was done, with measurement of 206 patients who received estrogenic treatment. In the Climacteric Clinic of the Instituto Nacional de Perinatologia two measurements were done of body composition, with DEXA kit, with one-year interval between them. The change in body mass index, weight and body fat of 206 patients was analyzed. The information was collected from April 2004 to June 2005. Results: Body mass index, weight, and total fat expressed in grams and the percentage of fat did not have significant changes in the evaluated patients who remained with the same ranges after receiving estrogenic treatment during one year before and after the study with DEXA. Conclusions: The evaluated patients did not experience changes in the body composition after a year among measurements with DEXA, during which they received estrogenic treatment. It is possible that estrogenic treatment maintains patients in their biophysical variables of body composition and avoids the natural tendency of increasing body fat caused by climacteric syndrome. [ABSTRACT FROM AUTHOR]
Published: 2006

40. Effect of Shoulder Movement Routine on Postoperative Shoulder Pain in Total Laparoscopic Hysterectomy: A Randomized Clinical Trial

Author: Andrea Olguín-Ortega, Lino Palacios-Cruz, Alejandro Rendón-Molina, Oliver Cruz-Orozco, Brenda Sánchez-Ramírez, Silvia Fabiola Estrada-Rivera, José Roberto Silvestri-Tomassoni, Ana Cristina Arteaga-Gómez, and Enrique Reyes-Muñoz
Subjects: laparoscopy hysterectomy, shoulder pain, postoperative pain, shoulder movement, Medicine (General), R5-920
Abstract: Background and Objectives: Postoperative shoulder pain is a common issue after total laparoscopic hysterectomy (TLH). This study evaluated the impact of a shoulder movement routine on postoperative shoulder pain in women undergoing uncomplicated TLH. Materials and Methods: An open-label randomized clinical trial included women without prior shoulder pain undergoing TLH between 20 January and 20 March 2024. Participants were randomized into two groups: Group 1 (n = 36) received a shoulder movement routine, while Group 2 (control, n = 39) performed a hand movement routine. Shoulder pain was assessed using the visual analog scale (VAS) at 6 h, 24 h, and 7 days postoperatively. Results: Seventy-five women participated. No significant differences were found between the groups regarding demographic variables, surgery duration, or hospital stay. Shoulder pain scores (VAS) at three time points (6 h, 24 h, and 7 days) showed no significant differences between groups (p = 0.57, p = 0.69, and p = 0.91, respectively). Similarly, there were no significant differences in incisional or abdominal pain. Conclusions: The shoulder movement routine did not significantly reduce postoperative shoulder pain in women undergoing uncomplicated TLH.
Published: 2024
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