4 results on '"Rivera-Kweh M"'
Search Results
2. Global TLR2 and 4 deficiency in mice impacts bone resorption, inflammatory markers and atherosclerosis to polymicrobial infection.
- Author
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Chukkapalli SS, Velsko IM, Rivera-Kweh MF, Larjava H, Lucas AR, and Kesavalu L
- Subjects
- Animals, Atherosclerosis etiology, Atherosclerosis immunology, Bone Resorption etiology, Bone Resorption immunology, Coinfection microbiology, Cytokines blood, Fusobacterium nucleatum immunology, Heat-Shock Proteins blood, Inflammation etiology, Inflammation immunology, Lipoproteins, LDL blood, Mice, Nitric Oxide blood, Periodontal Diseases microbiology, Porphyromonas gingivalis immunology, Tannerella forsythia immunology, Th2 Cells immunology, Toll-Like Receptor 2 genetics, Toll-Like Receptor 2 immunology, Toll-Like Receptor 4 genetics, Toll-Like Receptor 4 immunology, Treponema denticola immunology, Atherosclerosis physiopathology, Bone Resorption physiopathology, Coinfection immunology, Inflammation physiopathology, Toll-Like Receptor 2 deficiency, Toll-Like Receptor 4 deficiency
- Abstract
Toll-like-receptors (TLRs) play a significant role in the generation of a specific innate immune response against invading pathogens. TLR2 and TLR4 signaling contributes to infection-induced inflammation in periodontal disease (PD) and atherosclerosis. Observational studies point towards a relationship between PD and atherosclerosis, but the role of TLR2 and TLR4 in the recognition of multiple oral pathogens and their modulation of host response leading to atherosclerosis are not clear. We evaluated the role of TLR2 and TLR4 signaling in the induction of both PD and atherosclerosis in TLR2
-/- and TLR4-/- mice to polymicrobial infection with periodontal pathogens Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Polybacterial infections have established gingival colonization in TLR2-/- and TLR4-/- mice and induction of a pathogen-specific immunoglobulin G immune response. But TLR deficiency dampened accelerated alveolar bone resorption and intrabony defects, indicating a central role in infection-induced PD. Periodontal bacteria disseminated from gingival tissue to the heart and aorta through intravascular dissemination; however, there was no increase in atherosclerosis progression in the aortic arch. Polybacterial infection does not alter levels of serum risk factors such as oxidized low-density lipoprotein, nitric oxide, and lipid fractions in both mice. Polymicrobial-infected TLR2-/- mice demonstrated significant levels (P < 0.05 to P < 0.01) of T helper type 2 [transforming growth factor-β1 , macrophage inflammatory protein-3α, interleukin-13 (IL-13)] and T helper type 17 (IL-17, IL-21, IL-22, IL-23) splenic T-cell cytokine responses. Increased heat-shock protein expression, hspa1a for Hsp 70, was observed for both TLR2-/- and TLR4-/- mice. This study supports a role for TLR2 and TLR4 in PD and atherosclerosis, corroborating an intricate association between two inflammatory diseases., (© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)- Published
- 2017
- Full Text
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3. Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms.
- Author
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Venkatesh P, Phillippi J, Chukkapalli S, Rivera-Kweh M, Velsko I, Gleason T, VanRyzin P, Aalaei-Andabili SH, Ghanta RK, Beaver T, Chan EKL, and Kesavalu L
- Subjects
- Aged, Aortic Aneurysm, Abdominal diagnosis, Aortic Aneurysm, Thoracic diagnosis, Case-Control Studies, Cluster Analysis, Computational Biology methods, Female, Gene Expression Profiling, Genetic Association Studies, Humans, Male, Middle Aged, Risk Factors, Transcriptome, Aortic Aneurysm, Abdominal genetics, Aortic Aneurysm, Thoracic genetics, Genetic Predisposition to Disease, MicroRNAs genetics
- Abstract
Altered microRNA expression is implicated in cardiovascular diseases. Our objective was to determine microRNA signatures in thoracic aortic aneurysms (TAAs) and abdominal aortic aneurysms (AAAs) compared with control non-aneurysmal aortic specimens. We evaluated the expression of fifteen selected microRNA in human TAA and AAA operative specimens compared to controls. We observed significant upregulation of miR-221 and downregulation of miR-1 and -133 in TAA specimens. In contrast, upregulation of miR-146a and downregulation of miR-145 and -331-3p were found only for AAA specimens. Upregulation of miR-126 and -486-5p and downregulation of miR-30c-2*, -155, and -204 were observed in specimens of TAAs and AAAs. The data reveal microRNA expression signatures unique to aneurysm location and common to both thoracic and abdominal pathologies. Thus, changes in miR-1, -29a, -133a, and -221 are involved in TAAs and miR-145, -146, and -331-3p impact AAAs. This work validates prior studies on microRNA expression in aneurysmal diseases.
- Published
- 2017
- Full Text
- View/download PDF
4. Periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in B10.RIII mice.
- Author
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Chukkapalli S, Rivera-Kweh M, Gehlot P, Velsko I, Bhattacharyya I, Calise SJ, Satoh M, Chan EK, Holoshitz J, and Kesavalu L
- Subjects
- Animals, Mice, Periodontitis microbiology, Periodontium microbiology, Arthritis, Experimental microbiology, Arthritis, Experimental pathology, Periodontitis complications, Synovial Membrane microbiology
- Abstract
Background: It has been previously hypothesized that oral microbes may be an etiological link between rheumatoid arthritis (RA) and periodontal disease. However, the mechanistic basis of this association is incompletely understood. Here, we investigated the role of periodontal bacteria in induction of joint inflammation in collagen-induced arthritis (CIA) in B10.RIII mice., Methods: CIA-prone B10.RIII mice were infected orally with a polybacterial mixture of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia for 24 weeks before induction of CIA. The ability of polybacterial mixture to colonize the periodontium and induce systemic response, horizontal alveolar bone resorption in infected B10.RIII mice was investigated. Arthritis incidence, severity of joint inflammation, pannus formation, skeletal damage, hematogenous dissemination of the infection, matrix metalloproteinase 3 (MMP3) levels, and interleukin-17 expression levels were evaluated., Results: B10.RIII mice had gingival colonization with all three bacteria, higher levels of anti-bacterial immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies, significant alveolar bone resorption, and hematogenous dissemination of P. gingivalis to synovial joints. Infected B10.RIII mice had more severe arthritis, and higher serum matrix metalloproteinase 3 levels and activity. Histopathological analysis showed increased inflammatory cell infiltration, destruction of articular cartilage, erosions, and pannus formation. Additionally, involved joints showed had expression levels of interleukin-17., Conclusion: These findings demonstrate that physical presence of periodontal bacteria in synovial joints of B10.RIII mice with collagen-induced arthritis is associated with arthritis exacerbation, and support the hypothesis that oral bacteria, specifically P. gingivalis, play a significant role in augmenting autoimmune arthritis due to their intravascular dissemination to the joints.
- Published
- 2016
- Full Text
- View/download PDF
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