1. Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi
- Author
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Francine Govers, Marcus Dixon Law, Krista Gates, Cristina Cvitanich, Meng Zhang, Ulrich Gisi, Sophien Kamoun, Einat Yatzkan, Neil A. R. Gow, Thomas Deane Gaffney, Paul R. J. Birch, Edgar Huitema, Trudy Torto-Alalibo, Audrey M. V. Ah Fong, Mark E. Waugh, Pieter van West, Hemant Kelkar, Li Zheng, Rex Dwyer, Thomas A. Randall, Samuel Roberts, John David Windass, Thomas Boller, Howard S. Judelson, Andres Binder, Jun Yu, Elisabeth Mueller, Antonino Testa, Stephen Lam, Felix Mauch, Katinka Beyer, Randall, Ta, Dwyer, Ra, Huitema, E, Beyer, K, Cvitanich, C, Kelkar, H, Fong, Amva, Gates, K, Roberts, S, Yatzkan, E, Gaffney, T, Law, M, Testa, Antonino, Torto Alalibo, T, Zhang, M, Zheng, L, Mueller, E, Windass, J, Binder, A, Birch, Prj, Gisi, U, Govers, F, Gow, Na, Mauch, F, van West, P, Waugh, Me, Yu, J, Boller, T, Kamoun, S, Lam, St, and Judelson, Hs
- Subjects
late blight ,family ,Physiology ,UniGene ,artificial chromosome library ,Genome ,DNA, Algal ,Cluster Analysis ,DNA, Fungal ,Phylogeny ,Genetics ,Expressed Sequence Tags ,Expressed sequence tag ,codon usage ,Virulence ,EPS-2 ,food and beverages ,General Medicine ,Codon usage bias ,Phytophthora infestans ,Phytophthora ,DNA, Complementary ,Molecular Sequence Data ,Biology ,fusarium-graminearum ,chitin synthesis ,Evolution, Molecular ,Species Specificity ,Complementary DNA ,evolution ,Botany ,Amino Acid Sequence ,Codon ,Gene ,expressed sequences ,Gene Library ,Plant Diseases ,Polysaccharide-Lyases ,Sequence Homology, Amino Acid ,cDNA library ,Lysine ,Algal Proteins ,Fungi ,biology.organism_classification ,Laboratorium voor Phytopathologie ,Laboratory of Phytopathology ,cluster-analysis ,Agronomy and Crop Science ,pathogen - Abstract
To overview the gene content of the important pathogen Phytophthora infestans, large-scale cDNA and genomic sequencing was performed. A set of 75,757 high-quality expressed sequence tags (ESTs) from P. infestans was obtained from 20 cDNA libraries representing a broad range of growth conditions, stress responses, and developmental stages. These included libraries from P. infestans-potato and -tomato interactions, from which 963 pathogen ESTs were identified. To complement the ESTs, onefold coveragethe P. infestans genome was obtained and regions of coding potential identified. A unigene set of 18,256 sequences was derived from the EST and genomic data and characterized for potential functions, stage-specific patterns of expression, and codon bias. Cluster analysis of ESTs revealed major differences between the expressed gene content of mycelial and spore-related stages, and affinities between some growth conditions. Comparisons with databases of fungal pathogenicity genes revealed conserved elements of pathogenicity, such as class III pectate lyases, despite the considerable evolutionary distance between oomycetes and fungi. Thirty-seven genes encoding components of flagella also were identified. Several genes not anticipated to occur in oomycetes were detected, including chitin synthases, phosphagen kinases, and a bacterial-type FtsZ cell-division protein. The sequence data described are available in a searchable public database.
- Published
- 2005