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Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi
- Source :
- Randal, T A, Dwyer, R A, Huitema, E, Beyer, K, Cvitanich, C, Kelkar, H, Ah Fong, A M V, Gates, K, Roberts, S, Yatzkan, E, Gaffney, T, Law, M, Testa, A, Torto-Alalibo, T, Zhang, M, Zheng, L, Mueller, E, Windass, J, Binder, A, Birch, P R J, Gisi, U, Govers, F, Gow, N A, Mauch, F, van West, P, Waugh, M E, Yu, J, Boller, T, Kamoun, S, Lam, S T & Judelson, H S 2005, ' Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi. ', Mol. Plant-Microbe Interact., vol. 18, no. 3, pp. 229-243 . https://doi.org/10.1094/MPMI-18-0229, Molecular Plant-Microbe Interactions, 18(3), 229-243, Molecular Plant-Microbe Interactions 18 (2005) 3
- Publication Year :
- 2005
-
Abstract
- To overview the gene content of the important pathogen Phytophthora infestans, large-scale cDNA and genomic sequencing was performed. A set of 75,757 high-quality expressed sequence tags (ESTs) from P. infestans was obtained from 20 cDNA libraries representing a broad range of growth conditions, stress responses, and developmental stages. These included libraries from P. infestans-potato and -tomato interactions, from which 963 pathogen ESTs were identified. To complement the ESTs, onefold coveragethe P. infestans genome was obtained and regions of coding potential identified. A unigene set of 18,256 sequences was derived from the EST and genomic data and characterized for potential functions, stage-specific patterns of expression, and codon bias. Cluster analysis of ESTs revealed major differences between the expressed gene content of mycelial and spore-related stages, and affinities between some growth conditions. Comparisons with databases of fungal pathogenicity genes revealed conserved elements of pathogenicity, such as class III pectate lyases, despite the considerable evolutionary distance between oomycetes and fungi. Thirty-seven genes encoding components of flagella also were identified. Several genes not anticipated to occur in oomycetes were detected, including chitin synthases, phosphagen kinases, and a bacterial-type FtsZ cell-division protein. The sequence data described are available in a searchable public database.
- Subjects :
- late blight
family
Physiology
UniGene
artificial chromosome library
Genome
DNA, Algal
Cluster Analysis
DNA, Fungal
Phylogeny
Genetics
Expressed Sequence Tags
Expressed sequence tag
codon usage
Virulence
EPS-2
food and beverages
General Medicine
Codon usage bias
Phytophthora infestans
Phytophthora
DNA, Complementary
Molecular Sequence Data
Biology
fusarium-graminearum
chitin synthesis
Evolution, Molecular
Species Specificity
Complementary DNA
evolution
Botany
Amino Acid Sequence
Codon
Gene
expressed sequences
Gene Library
Plant Diseases
Polysaccharide-Lyases
Sequence Homology, Amino Acid
cDNA library
Lysine
Algal Proteins
Fungi
biology.organism_classification
Laboratorium voor Phytopathologie
Laboratory of Phytopathology
cluster-analysis
Agronomy and Crop Science
pathogen
Subjects
Details
- ISSN :
- 08940282
- Volume :
- 18
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Accession number :
- edsair.doi.dedup.....f014a5632ff517e3051b0b8f48798b7e