Search

This perspective explores the transformative potential of data-driven insights to understand and address women's reproductive health conditions. Historically, clinical studies often excluded women, hindering comprehensive research into conditions such as adverse pregnancy outcomes and endometriosis. Recent advances in technology (e.g., next-generation sequencing techniques, electronic medical records (EMRs), computational power) provide unprecedented opportunities for research in women's reproductive health. Studies of molecular data, including large-scale meta-analyses, provide valuable insights into conditions like preterm birth and preeclampsia. Moreover, EMRs and other clinical data sources enable researchers to study populations of individuals, uncovering trends and associations in women's reproductive health conditions. Despite these advancements, challenges such as data completeness, accuracy, and representation persist. We emphasize the importance of holistic approaches, greater inclusion, and refining and expanding on how we leverage data and computational integrative approaches for discoveries so that we can benefit not only women's reproductive health but overall human health.

Case history: In 2023, the New Zealand Department of Conservation seized 63 endemic reptiles that were being held without a permit. This group included three adult female West Coast green geckos (Naultinus tuberculatus) that had been illegally removed from the wild 2 years earlier. They had been held in an outdoor enclosure with a pair of goldstripe geckos (Woodworthia chrysosiretica). Clinical findings: On physical examination, all three geckos had at least two soft palpable masses in the coelom. Repeated ultrasonographic examination over several months confirmed the diagnosis of pre-ovulatory follicular stasis (POFS) in each gecko, and in subsequent weeks, more ovarian follicles developed in each animal. Laboratory findings: All three geckos were negative on culture of cloacal swabs for Salmonella spp., and negative on PCR assay of a cloacal flush for Cryptosporidium spp., despite other reptiles in the seized group showing positive results for multiple Salmonella spp., and one other gecko being positive for Cryptosporidium parvum, subtype IIcA5G3. Treatment and outcome: For all three geckos, para-midline ventral coeliotomy was performed under general anaesthesia, and folliculectomy of degenerate ovarian follicles was performed. Post-operative complications were seen in all three animals, which developed suture-line infections following disruption of normal skin shedding and entrapment of shed keratin in the surgical sites. A second surgery was undertaken to remove impacted keratin and caseous inflammatory material from the surgical wounds of all three animals and buried sutures were placed to close the coelomic wounds. The geckos were treated with 20 mg/kg ceftazidime IM every second day for 2 weeks post-operatively. Subsequent ecdysis (skin shedding) occurred without complication and the geckos were released back to the wild 10 months after admission. Clinical relevance: The recommended treatment for POFS in reptiles is ovariectomy, which is not appropriate for wild animals. The use of folliculectomy to resolve preovulatory follicular stasis should be considered for animals where retaining reproductive ability is essential. [ABSTRACT FROM AUTHOR]

Macrobrachium acanthurus is an amphidromous palaemonid shrimp exploited by artisanal fishing in Brazil that presents limited published information on its population biology. Therefore, this study aimed to assess the spatial distribution and fecundity of M. acanthurus in the Jequitinhonha River (BA). Collections occurred at four points in the river downstream of the Itapebi Hydroelectric Powerplant (P1–P4). The abundance and size of the demographic categories analysed (male morphotypes, non-ovigerous females, and ovigerous females) and fecundity were compared among points. We also examined the relationship between female body size and fecundity. A total of 938 individuals were collected during the sampling period. The highest abundances were observed downstream (P3 and P4). The sizes of males and ovigerous females were greater at points farthest upstream. Three male morphotypes were identified, and their proportions varied among points. Fecundity was higher upstream, and there was a positive correlation between female size and number of eggs. All population parameters indicate a longitudinal gradient towards the estuary, with strong evidence of amphidromy. This is the first study with a polymorphic species to consider morphotypes as a population structure component. The results represent an important contribution to a better understanding of the amphidromous life cycle of palaemonid shrimp. [ABSTRACT FROM AUTHOR]

Heterostyly is a specialized reproductive system associated with self‐ and intramorph incompatibility. It reinforces the intermorph breeding system in heterostylous plant species. However, distyly has not been comprehensively studied in Nyctanthes arbor‐tristis. The present study demonstrates the floral traits, breeding system, fruit and seed set, and morph ratio in distylous N. arbor‐tristis. The length of style and filament showed reciprocal herkogamy between long style (L‐morph) and short style (S‐morph). Flower size, corolla tube size, stamen and anther length, ovary length, and pollen diameter were larger in S‐morph. However, the style was longer in L‐morph. Our results demonstrated strongly incompatible self‐ and intramorph pollination combinations. The intermorph pollination was compatible, showing normal pollen tube growth throughout the style and ovary. The embryo formation was also higher in intermorph interaction. Moreover, the fruit setting was higher when the intermorph plants grew in the vicinity of natural conditions. Collectively, all data indicate that N. arbor‐tristis is a distylous species with a self‐incompatible breeding system, resulting in less seed formation in nature. [ABSTRACT FROM AUTHOR]

For the live-bearing and egg-laying class of chondrichthyan fishes a three parameter logistic 'maternity' function with a variable upper asymptote, PMax, can be used to predict the average probability of a female giving birth or laying eggs in a season. Although fundamental to calculating the reproductive capacity of a population, few studies report maternity functions, with maturity functions often used as a proxy. Applying logistic models to simulated and empirical data showed that it was feasible to estimate PMax from maternal data and that accuracy, bias, and confidence interval coverage often improved compared to when a fixed value was used. However, sample sizes of 100–200 maternal females were typically required for accurate estimation of PMax. While maturity parameters could be estimated with greater accuracy, substituting them for maternity parameters overestimated lifetime reproductive output. Greater use of maternity functions has the potential to improve calculation of reproductive output in quantitative populations models. In addition to improvements in parameter estimation, this method involves fewer assumptions and enables statistical inferences to be made on frequency of reproduction. [ABSTRACT FROM AUTHOR]

Small-sized fishes play a crucial ecological role in the dynamics of riverine systems, but their low dispersal capacity and high endemism make them highly vulnerable to environmental change. In the Neotropical region, each watershed typically supports unique populations of small-sized fishes, and in this study, we used Psalidodon paranae (Characidae) as a model to understand the relationships between its population patterns (including age, growth, and reproductive biology) and environmental characteristics. Standardised monthly sampling of P. paranae was carried out at ten sampling sites from June 2010 to May 2011, and environmental variables (including water quality and river characteristics) were measured to characterise these sites. Multivariate techniques and generalised linear models were used to analyse the longitudinal distribution of P. paranae, which was significantly influenced by river depth and width, and suspended solid concentrations. Linear and non-linear models were used to describe the length–weight relationship (resulting in an isometric pattern, b = 3.0256) and the growth patterns (L∞ = 21.8 cm, k = 0.51 year−1, and Φ′ = 2.3989). The analysis of gonadal maturity was combined with somatic and reproductive indices, allowing the identification of spawning periods (occurring mainly during the austral summer) and areas (sites with headwater characteristics). Our results indicate that P. paranae has a good adaptability to the different environmental conditions, and this first assessment of age and growth parameters constitutes a reference for monitoring and further evaluation of long-term changes. [ABSTRACT FROM AUTHOR]

This pioneering study aimed to evaluate the cryopreservation of semen from P. falkneri (n = 4) and P. motoro (n = 4), maintained ex situ at the Sao Paulo Aquarium, Brazil. For this purpose, the animals were physically restrained, biometric data of the disc and clasper were obtained, and semen was collected through manual massage. Total motility and progressive motility parameters were evaluated using Computer-Assisted Sperm Analysis (CASA) with IVOS II equipment and Animal Breeders II software. The semen extenders INRA 96 and OptiXcell were used to assess their efficacy in sperm cryopreservation. INRA required the addition of 5% dimethyl sulfoxide (DMSO) as a cryoprotectant. The results indicated that there was no difference in semen motility values before and after freezing with INRA + DMSO (p = 0.6226). On the other hand, samples cryopreserved with OptiXcell showed a difference in semen motility post-thaw (p = 0.0156). These findings contribute to a broader study on optimizing cryopreservation protocols to ensure long-term viability and fertility of semen, enhancing genetic diversity and supporting wild population restoration. A multidisciplinary approach integrating reproductive biology, ecology, physiology, and assisted reproduction technologies, aligned with the One Conservation concept, is essential for advancing conservation and management strategies for these threatened species.

The floral microenvironment impacts gametophyte viability and plant–pollinator interactions. Plants employ mechanisms to modify floral temperature, including thermogenesis, absorption of solar radiation, and evaporative cooling. Whether floral thermoregulation impacts reproductive fitness, and how floral morphological variation mediates thermoregulatory capacity are poorly understood.We measured temperature of the floral microenvironment in the field and tested for thermogenesis in the lab in early spring flowering Hexastylis arifolia (Aristolochiaceae). We evaluated whether thermoregulatory capacity was associated with floral morphological variation. Finally, we experimentally determined the thermal optimum and tolerance of pollen to assess whether thermoregulation may ameliorate thermal stress to pollen.Pollen germination was optimal near 21 °C, with a 50% tolerance breadth of ~18 °C. In laboratory conditions, flowers exhibited thermogenesis of 1.5–4.8 °C for short intervals within a conserved timeframe (08:00–09:00 h). In the field, temperature inside the floral tube often deviated from ambient – floral interiors were up to 4 °C above ambient when it was cold, but some fell nearly 10 °C below ambient during peak heat. Flowers with smaller openings were cooler and more thermally stable than those with larger openings during peak heat. Thermoregulation maintained a floral microenvironment within the thermal tolerance breadth of pollen.Results suggest that H. arifolia flowers have a stronger capacity to cool than to warm, and that narrower floral openings create a distinct floral microenvironment, enhancing floral cooling effects. While deviation of floral temperature from ambient conditions maintains a suitable environment for pollen and suggests an adaptive role of thermoregulation, we discuss adaptive and nonadaptive mechanisms underlying floral warming and cooling. [ABSTRACT FROM AUTHOR]

Background Objectives Materials and methods Results Discussion and conclusion Severe acute respiratory syndrome coronavirus 2, the virus responsible for coronavirus disease 2019, affects multiple organs. The virus enters cells through angiotensin‐converting enzyme‐2 and host factors present in genital organs, leading to concern over virus shedding in semen and reproductive function.To investigate severe acute respiratory syndrome coronavirus 2 in semen from patients with a mild infection, identify the seminal infected cells, and explore the effect of the infection on sex hormones and semen parameters.Prospective study of 54 men with mild severe acute respiratory syndrome coronavirus 2 infection. Semen was collected at 7, 15, 30, 60, 90, 180, and 365 days after symptom onset, and severe acute respiratory syndrome coronavirus 2 RNA was measured in serum, saliva, urine, and semen. The presence of infectious severe acute respiratory syndrome coronavirus 2 in semen was assessed using Vero cell culture. Infected semen cells were identified using immunofluorescence against severe acute respiratory syndrome coronavirus 2 nucleoprotein antigen and cell markers. Semen characteristics as well as testosterone, inhibin B, luteinizing hormone, and follicle‐stimulating hormone levels were determined.11% of patients had at least one severe acute respiratory syndrome coronavirus 2 RNA‐positive semen. One patient had viral semen shedding up to day 90 after infection onset, with replication‐competent virus isolated from semen and 40% cell fraction at day 7. After sperm preparation, 90% fraction was severe acute respiratory syndrome coronavirus 2 RNA‐positive at days 7 and 15. The swim‐up fraction was positive only on day 7. In semen, nucleoprotein antigen was detected mainly in exfoliated epithelial cells and less frequently in Sertoli cells. Sperm count and motile sperm count were lower at day 30 than at day 7. Round cells in semen were increased during the acute phase. At days 7 and 15, sperm count and motile sperm count were lower in severe acute respiratory syndrome coronavirus 2 RNA‐positive semen compared with negative semen, while semen volume and follicle‐stimulating hormone levels were increased. Long‐term follow‐up shows no evidence of a detrimental effect on hormonal or semen characteristics.11% of patients with mild coronavirus disease 2019 who were not hospitalized had severe acute respiratory syndrome coronavirus 2 excretions in semen, which persisted for up to 90 days in one patient. No germ cells appeared infected by the virus, but the detection of nucleoprotein antigen‐positive epithelial semen cells and Sertoli cells suggests genital tract infection. Albeit infrequent, semen may contain the replication‐competent virus during the acute phase with potential risk of severe acute respiratory syndrome coronavirus 2 transmissions during sexual contact and assisted reproduction procedures. The effect of mild coronavirus disease 2019 on spermatogenesis and reproductive hormones was moderate and reversible. [ABSTRACT FROM AUTHOR]

This account presents information on all aspects of the biology of Ceratonia siliqua L. (Carob) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the International Biological Flora: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation.Ceratonia siliqua is a lowland evergreen shrub or tree, native around the Mediterranean basin and widely cultivated in areas with a warm temperate and subtropical climate, with more than 100 named cultivars. Carob grows on a wide range of soils including nutrient‐poor and strongly calcareous, dry soils.Carob is dioecious or rarely hermaphrodite although females dominate in cultivation due to their fruit. Pollination is primarily by insects and the relatively large seeds are spread mainly by ingestion of fruits by mammals. Seed dormancy is imposed by a hard seed coat and seeds germinate readily after natural or artificial scarification.Drought tolerance is very high, acting as a drought avoiding water spender. Carob is also very tolerant of saline conditions and tolerant of fire and can act to restrict wildfire spread by generating fuel discontinuity. It shows high post‐fire regrowth. Old individuals house a diversity of associated fauna and are comparatively unaffected by serious pests and diseases.Carob has a long cultural history around the Mediterranean as a food source for humans and domesticated animals, in herbal and modern medicine and, more recently, for the carob bean gum used in a variety of domestic products and foods. Commercial production of fruits has declined over past decades but the low cultural care needed in growing orchards, potential new markets for fruit and seeds, and growing use of the tree to prevent soil erosion and sequester carbon gives carob a brighter future. [ABSTRACT FROM AUTHOR]

Studies about reproductive aspects of angiosperms rarely refer to herbaceous and anemophilous groups. Cyperaceae are cosmopolitan and diverse in terms of sexuality, inflorescence architecture, and pollination modes, such as anemophily, entomophily, and ambophily. Therefore, the evolution of reproductive traits can clarify some questions about the reproductive biology of angiosperms, especially regarding the transitions between biotic and abiotic pollination. This study was designed to investigate the evolution of reproductive traits in Cyperaceae through comparative phylogenetic methods, such as reconstruction of ancestral states and evolutionary correlation. If spicoids in Mapanioideae are indeed inflorescences, the bisexual flower in Cyperaceae would be derived, a rare case in angiosperms. In Cyperoideae, a decrease in the number of flowers and an increase in stamen loss events were observed. This result contradicts the hypothesized increase in reproductive structures in anemophilous species, in which a greater amount of pollen is produced to compensate for the waste generated by wind. Only one correlation was found: between the number of inflorescence branches and the number of reproductive units per inflorescence. Finally, the analyses showed the great diversity of reproductive biology in Cyperaceae and made evident the need to expand the theoretical studies to refine the discovery of correlated traits. [ABSTRACT FROM AUTHOR]

Background and Aims The deceptive strategies by which orchids are pollinated and how these are capable of attracting pollinators remain understudied with regard to their implications for plant fitness. Despite their ecological importance, limited investigations have been conducted on sexual deception and shelter mimicry in orchid species, making this a compelling avenue in orchid biology research. To expand the knowledge of these reproductive mechanisms, we studied the pollination of Serapias lingua and S. parviflora in co-occurring and isolated sites in the Balearic Islands (Spain), further accentuated by the presence of a hybrid, indicating shared pollinators. Methods We employed bagging and hand pollination experiments to examine the reproductive biology of the two species. Furthermore, we evaluated the influence of phenotypical and ecological factors on reproductive success, including biometric measurements, reproductive performance and neighbourhood diversity (co-flowering and pollinator communities). Key Results Reproductive mechanisms between these two orchid species exhibit substantial disparities. Serapias lingua relies primarily on insect-mediated pollination, while S. parviflora demonstrates self-reproduction capacity. Although events of open pollination are rare, hybridization occurs predominantly when S. lingua is the pollen donor. Fruit set in S. parviflora was positively correlated with plant height, while in S. lingua it was negatively associated with flower size. The coexistence of the two species positively affected pollinium removal in S. parviflora , but did not exert an influence on reproductive traits in S. lingua. Overall, biometric parameters were diminished in isolated compared with co-occurring sites. At the community level, the increased diversity of co-flowering species in the vicinity exhibited an inhibitory effect on pollinium removal in S. parviflora. Conclusions Under a context of pollinator loss or phenological mismatch between pollinator presence and flowering, the selfing capacity of S. parviflora would guarantee reproduction whereas S. lingua survival would be compromised. Furthers studies are needed to assess the effects of phenotypical and ecological factors on reproductive success of S. lingua in pollinator-decline scenarios. [ABSTRACT FROM AUTHOR]

Context: Published evidence suggests recruitment of floodplain trees is declining in the northern Murray–Darling Basin (MDB) and that this is linked to river flow. Aims: We investigated the reproductive phenology of Eucalyptus camaldulensis and Eucalyptus coolabah to better understand tree reproductive cycles and the influence of rainfall and flow on seed production. Methods: We surveyed phenological events (bud, flower and fruit production) over 6 years, at nine sites along the Condamine–Balonne River and examined correlations between these events and meteorological parameters. Key results: For both species, event timing was consistent across sites and years and event relative abundance scores were different between years. Bud abundance was correlated with rainfall, flooding and warming temperatures and E. coolabah produced additional bud crops after floods. Conclusions: The timing of events did not differ significantly from published results for the southern MDB. Rainfall, river flows and flooding in spring resulted in more abundant bud crops in E. camaldulensis and E. coolabah , and subsequently more abundant fruit crops. Fruit dehiscence coincided with summer–autumn flooding, which provides seed for germination. Genetic and physiological factors may also influence the observed pattern. Implications: These results will inform water planning in Queensland, Commonwealth Environmental Water Holder watering actions and long-term watering plans in the northern MDB. Eucalyptus camaldulensis and Eucalyptus coolabah are floodplain trees found in the Murray–Darling Basin (MDB). This long-term study observed the timing and abundance of buds, flowers and fruits at sites in the northern MDB and looked for patterns that would indicate the influence of river flows. Bud abundance, canopy seed storage and seed fall were influenced by moisture availability in both species. Image credit: J. L. Kerr. This article belongs to the collection Environmental flows in northern Murray–Darling Basin: what we know about the science and management after a decade of practice. [ABSTRACT FROM AUTHOR]

Data on the reproductive biology of elasmobranchs are essential for understanding their life history. Published studies on batoid ray reproductive biology are comparatively scarce, leading to limited understanding and data gaps. The Oceania fantail ray, Taeniura lessoni, is a good example. This Data Deficient nearshore stingray is restricted to Melanesia, with lacking biological and ecological data, including reproduction. To expand upon the limited life-history data for this species, this short paper provides observational data on the reproductive condition in female T. lessoni, at Drawaqa Island, Fiji. Field work involved direct observations and ocean temperature measurements. Over 40 days spanning three months, 105 surveys were conducted across five sites, resulting in 71 sightings of the species. Based on spot patterns and body markings, four female individuals were identified. Between January and March 2024, these females exhibited convex dorsa indicating advanced gestation, transitioning to concave dorsa suggesting parturition. The presence of neonates from early March onwards coincided with the estimated parturition period inferred from the rays' condition. Furthermore, a female previously pregnant was photographed with a dermal abrasion around her pectoral fin, possibly indicating pre-copulatory biting, suggesting a continuous reproductive cycle. The average monthly water temperature at the surveyed sites remained relatively stable throughout the study. Collectively, our findings suggest that Drawaqa Island provides suitable habitat niches for reproductive activities in female T. lessoni. Repeated and long-term data is certainly needed to confirm either a continuous reproductive cycle or seasonal peaks. While preliminary, our observational data represents the first documentation on female reproductive condition in a stingray in Fiji. [ABSTRACT FROM AUTHOR]

The objective of this study was to characterize the reproductive compatibility among cultivars and selections of Japanese plum through controlled pollination, both in vivo and in situ, to obtain a profile of reproductively compatible cultivars. In situ and in vivo self-pollinations were carried out in an F1 population of Japanese plum, and in vivo pollinations were conducted on the pollen-grain receptor cultivars: Laetitia, Zafira and Fortune, and the pollen-grain donor cultivars: Piamontesa, Selection Plum 86-13, Harry Pickstone, Black Amber, Bruce, SC-17, Laetitia, SC-15, SC 7, Zafira, Amarelinha, América, Reubennel and Fortune, to verify the percentage of germination of pollen grains and growth of pollen tubes in the pistils of the recipient plants. The in situ and in vivo assays for the F1 population confirm the self-compatibility of genotypes 13 and 35. The Laetitia cultivar is self-incompatible; however, it is compatible with the Selection 'Plum 86-13'. 'Zafira' is self-incompatible. 'Fortune' is compatible with 'Amarelinha', 'SC-7', 'America', 'Harry Pickstone' and 'Reubennel', but it is self-incompatible. [ABSTRACT FROM AUTHOR]

Ex vivo follicle growth is an essential tool, enabling interrogation of folliculogenesis, ovulation, and luteinization. Though significant advancements have been made, existing follicle culture strategies can be technically challenging and laborious. In this study, we advanced the field through development of a custom agarose micromold, which enables scaffold-free follicle culture. We established an accessible and economical manufacturing method using 3D printing and silicone molding that generates biocompatible hydrogel molds without the risk of cytotoxicity from leachates. Each mold supports simultaneous culture of multiple multilayer secondary follicles in a single focal plane, allowing for constant timelapse monitoring and automated analysis. Mouse follicles cultured using this novel system exhibit significantly improved growth and ovulation outcomes with comparable survival, oocyte maturation, and hormone production profiles as established three-dimensional encapsulated in vitro follicle growth (eIVFG) systems. Additionally, follicles recapitulated aspects of in vivo ovulation physiology with respect to their architecture and spatial polarization, which has not been observed in eIVFG systems. This system offers simplicity, scalability, integration with morphokinetic analyses of follicle growth and ovulation, and compatibility with existing microphysiological platforms. This culture strategy has implications for fundamental follicle biology, fertility preservation strategies, reproductive toxicology, and contraceptive drug discovery. [ABSTRACT FROM AUTHOR]

Miscanthus ×giganteus (giant miscanthus), first found as a naturally occurring hybrid, has shown promise as a bioenergy/biomass crop throughout much of the temperate world. This allotriploid (2n = 3x = 57) hybrid resulted from a cross between tetraploid Miscanthus sacchariflorus (2n = 4x = 76) and diploid Miscanthus sinensis (2n = 2x = 38) and is particularly desirable due to its low fertility that minimizes reseeding and potential invasiveness. However, there is limited genetic diversity in commonly grown cultivars of triploid M. ×giganteus and breeding and development efforts to improve and domesticate this crop have been minimal. Here, we report on newly developed M. ×giganteus hybrids compared with the industry standard M. ×giganteus '1993‐1780'. Dry biomass yields of new hybrids ranged from 19.5 to 32.4 Mg/ha/year for the fourth growing season, compared with 21.0 Mg/ha/year for M. ×giganteus '1993‐1780'. Plant reproductive fertility remained low for all accessions with overall fertility [(seed set × seed germination)/100] ranging from 0.3% to 4.5% for new hybrids compared to 0.4% for M. ×giganteus '1993‐1780'. Culm density and height varied among accessions and were positively correlated with increased biomass. Based on compositional analyses, theoretical ethanol yields ranged from 9, 740 to 16,278 L/ha/year for new hybrids compared to 10,406 L/ha/year for M. ×giganteus '1993‐1780'. Relative feed value indices were low overall and ranged between 66.0 and 72.8 for new hybrids compared to M. ×giganteus '1993‐1780' with 71.3. The genetic diversity of new hybrids, compared with existing cultivars, was characterized using whole genome sequences. Based on pair‐wise distances, cluster analysis clearly showed increased diversity of new hybrids compared with earlier selections. These results document new triploid hybrids of M. ×giganteus with enhanced biomass and theoretical ethanol yields in combination with broader genetic diversity and lowreproductive fertility. [ABSTRACT FROM AUTHOR]

In this work, we introduce a compartmental model of ovarian follicle development all along lifespan, based on ordinary differential equations. The model predicts the changes in the follicle numbers in different maturation stages with aging. Ovarian follicles may either move forward to the next compartment (unidirectional migration) or degenerate and disappear (death). The migration from the first follicle compartment corresponds to the activation of quiescent follicles, which is responsible for the progressive exhaustion of the follicle reserve (ovarian aging) until cessation of reproductive activity. The model consists of a data-driven layer embedded into a more comprehensive, knowledge-driven layer encompassing the earliest events in follicle development. The data-driven layer is designed according to the most densely sampled experimental dataset available on follicle numbers in the mouse. Its salient feature is the nonlinear formulation of the activation rate, whose formulation includes a feedback term from growing follicles. The knowledge-based, coating layer accounts for cutting-edge studies on the initiation of follicle development around birth. Its salient feature is the co-existence of two follicle subpopulations of different embryonic origins. We then setup a complete estimation strategy, including the study of structural identifiability, the elaboration of a relevant optimization criterion combining different sources of data (the initial dataset on follicle numbers, together with data in conditions of perturbed activation, and data discriminating the subpopulations) with appropriate error models, and a model selection step. We finally illustrate the model potential for experimental design (suggestion of targeted new data acquisition) and in silico experiments. [ABSTRACT FROM AUTHOR]

Plants that bloom in early spring often face constraints on reproduction, which are influenced by low temperatures and unpredictable weather conditions that affect pollinator activity. We aimed to elucidate the pollination ecology and the mating system of Ranzania japonica (Berberidaceae), a perennial plant of a monotypic genus endemic to deep‐snow regions in Japan. We documented the flowering phenology and visiting insects in the field, and conducted pollination experiments by using artificial treatments. R. japonica was suggested to be self‐incompatible but not pollen‐limited. Fruit set varied between years, and florivory seemed to have a negative influence on fruit set. Insects from various taxa were observed to visit the flowers of R. japonica, with Eusphalerum sp. (rove beetle) being the most abundant, followed by Bombylius major and hymenopteran species. The stamen movement in response to insect visits was observed. The results of the observation and the pollination treatments suggested that small‐ and medium‐sized Hymenoptera were the main pollinators and rove beetles may also contribute to pollination in R. japonica. Because early spring weather can be unsuitable for activity of bees, rove beetles may act as supplementary pollinators of R. japonica. The role of the supplementary pollinators may be an overlooked but important aspect for understanding the pollination biology of early‐spring blooming plants. [ABSTRACT FROM AUTHOR]

Mud crabs are common edible crustaceans, which are fished along the coasts of India. Scylla tranquebarica (Fabricius 1798), commonly called green crab is one of the most economically important portunid crab in India. The study was carried out to determine the sex ratio, maturation and spawning, gonadosomatic index, fecundity and egg diameter. Sex ratio of 1 female to 1.3 male gave good results. Size at first maturity was observed at 11 cm carapace width (CW) in males and 10 cm (CW) in females. The highest GSI values in males (6.17) and females (5.12) were observed in the month of October. The maximum number of eggs produced by the females was up to 5 million. The egg diameter was recorded in the range of 0.69 to 0.76 mm. The incubation period was 11-13 days. The hatching success average percentage was recorded as 80%. For the incubation and larval rearing of mud crab, Scylla tranquibarica, the optimum requirement of water temperature and salinity were recorded as 28°C and 30 ppt, respectively. [ABSTRACT FROM AUTHOR]

BACKGROUND: Ambrosia grayi is a perennial weed native to northern Mexico, which can also be found in the Great Plains of the US. Outside the Americas, A. grayi has only been documented in Israel, where it is currently categorized as a casual species at advanced eradication stages. Here, we studied the plant biology and chemical weed management options of A. grayi. RESULTS: Only large achenes of A. grayi (~5% of all achenes) contain seeds; moreover, the viability of seeds extracted from large achenes was ~25%. Examination of plant anatomy revealed that underground vegetative segments show an anatomical structure of stems (rhizomes) with anomalous secondary growth. The optimal (night/day) temperature for the emergence of A. grayi rhizomes was 20/30 °C, and the emergence rate increased under elevated temperatures. Emergence may occur at different soil moisture content (25–60%); rhizomes were able to emerge even after 1 month of drought conditions (20%, 25% and 30%). Herbicide combinations, such as fluroxypyr + glufosinate, fluroxypyr + glyphosate, and glyphosate + saflufenacil + surfactant, were tested under quarantine conditions and showed high efficacy for the control of A. grayi. However, the efficiency of these treatments was highly correlated with plant growth stage. CONCLUSION: In Israel, the spread of A. grayi occurs mainly via rhizomes that can emerge under a wide range of temperatures and soil moisture conditions. Data regarding herbicide efficacy will aid in improving the eradication efforts taken by Israel's Plant Protection and Inspection Services. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Copyright of Biotropica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anatolian Environmental & Animal Science is the property of Bulent Verep and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This pioneering study aimed to evaluate the cryopreservation of semen from P. falkneri (n = 4) and P. motoro (n = 4), maintained ex situ at the Sao Paulo Aquarium, Brazil. For this purpose, the animals were physically restrained, biometric data of the disc and clasper were obtained, and semen was collected through manual massage. Total motility and progressive motility parameters were evaluated using Computer-Assisted Sperm Analysis (CASA) with IVOS II equipment and Animal Breeders II software. The semen extenders INRA 96 and OptiXcell were used to assess their efficacy in sperm cryopreservation. INRA required the addition of 5% dimethyl sulfoxide (DMSO) as a cryoprotectant. The results indicated that there was no difference in semen motility values before and after freezing with INRA + DMSO (p = 0.6226). On the other hand, samples cryopreserved with OptiXcell showed a difference in semen motility post-thaw (p = 0.0156). These findings contribute to a broader study on optimizing cryopreservation protocols to ensure long-term viability and fertility of semen, enhancing genetic diversity and supporting wild population restoration. A multidisciplinary approach integrating reproductive biology, ecology, physiology, and assisted reproduction technologies, aligned with the One Conservation concept, is essential for advancing conservation and management strategies for these threatened species. [ABSTRACT FROM AUTHOR]

Though a previous study in Sudan confirmed the existence of a living hybrid of an oceanic manta ray (Mobula birostris) and a reef manta ray (M. alfredi), courtship behaviors between the two closely related species have never been documented. Here we report the first observation of courtship behaviors between two male M. birostris and a female M. alfredi at a manta ray cleaning station on a shallow coral reef seamount in Misool, Raja Ampat, Indonesia. The observed courtship event lasted for approximately 45 min and involved one male oceanic manta actively chasing the mature reef manta female around the cleaning station and repeatedly bumping her on the left wing tip with his right cephalic lobe, while the second male continuously flanked the female to seemingly prevent her retreat from the cleaning station and potential evasion of the pursuing male. Notably, the female exhibited a rapid coloration change, likely indicative of courtship-related stress, transitioning from the striking pale hue she exhibited throughout the courtship activity to a normal chevron pattern once the males departed. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Dalian Ocean University is the property of Journal of Dalian Ocean University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

It has been assumed that functional maturity and terminal molt occur simultaneously in majoid crabs. Although this assumption seems to be accurate in females, males of some majoids can mate with females even before their terminal molt. Here we describe the morphometric changes at terminal molt and test the reproductive capacity of pre-terminal molt males in the kelp crab Pugettia ferox Ohtsuchi & Kawamura, 2019 (Majoidea, Epialtidae, Epialtinae). We measured the post-pseudorostral carapace length (PCL), claw length (CL), claw height (CH), and pleon (abdomen) width (AW) of crabs to estimate the morphological features distinguishing pre- and post-terminal molt individuals, namely relative claw length (CL/PCL) ≥ 0.660 in males and relative abdominal width (AW/PCL) ≥ 0.487 in females. Males with pre-terminal molt morphological features were observed for behavioral maturity through encounters with mature virgin females under laboratory conditions. More than half of the males copulated with a female and then underwent terminal molt. These females bred clutches, and larvae hatched. These results indicate that pre-terminal molt males of P. ferox can be functionally mature. [ABSTRACT FROM AUTHOR]

Frogs of the Allophrynidae are an enigmatic family from South America. To date, published information is lacking regarding this group's reproductive biology and larval morphology. Here, we provide the first detailed description of the reproductive mode, developmental mode, and tadpole morphology for Allophryne ruthveni. We developed a captive breeding and rearing protocol for this species and then conducted a series of observations to describe aspects of its reproductive biology. In captivity, this species exhibits aquatic oviposition, where single eggs are laid ungrouped within a simple jelly capsule and are scattered free in the water column before sinking to develop on benthic substrates. We did not observe parental care nor any parental interactions with eggs post-fertilization. Tadpoles are characterized by an oval body, anteroventral oral disc, a labial tooth row formula of 2(2)/3, and a dextral vent tube. The buccopharyngeal cavity is marked by the presence of two pairs of infralabial papilla and four lingual papillae. Cranial morphology is characterized by the presence of the commissura quadratoorbital. This species possesses an additional slip of the m. rectus cervicis and of the m. levator arcuum branchialium III. We discuss our results in comparison with glassfrogs (Centrolenidae). [ABSTRACT FROM AUTHOR]

Snakes represent a wide and diverse group of species and have anatomical particularities, such as the renal sexual segment (RSS), a structure located in the kidneys and formed from the hypertrophy of the urinary ducts and nephrons. This study aims at describing the histological aspects of the RSS of Boa constrictor, Epicrates cenchria and Corallus hortulanus, all of which are Brazilian snake species from the Boidae family. The reproductive system and kidneys of five male specimens of E. cenchria, three male specimens of C. hortulanus and two male specimens of B. constrictor were obtained. Tissue samples were processed histologically and different stains used (Toluidine Blue, Alcian Blue and Periodic Acid Schiff). The histological evaluation of the RSS of E. cenchria, C. hortulanus and B. constrictor shows that the RSS in these species varies when comparing individuals in the reproductive period with those which are not. It also allows for the observation of the segment's secretory activity in animals in the reproductive stage (mature sperm in the lumen of the seminiferous tubules) as well as in those which are not. Finally, the histological evaluation also reveals the variation of the secretion product in individuals in the reproductive period, in those which are not, and also among individuals within the same reproductive stage. [ABSTRACT FROM AUTHOR]

The insect ion transport peptide (ITP) and its alternatively spliced variant, ITP-like peptide (ITP-L), belong to the crustacean hyperglycemic hormone family of peptides and are widely conserved among insect species. While limited, studies have characterized the ITP/ITP-L signaling system within insects, and putative functions including regulation of ion and fluid transport, ovarian maturation, and thirst/excretion have been proposed. Herein, we aimed to molecularly investigate Itp and Itp-l expression profiles in the mosquito, Aedes aegypti, examine peptide immunolocalization and distribution within the adult central nervous system, and elucidate physiological roles for these neuropeptides. Transcript expression profiles of both AedaeItp and AedaeItp-l revealed distinct enrichment patterns in adults, with AedaeItp expressed in the brain and AedaeItp-l expression predominantly within the abdominal ganglia. Immunohistochemical analysis within the central nervous system revealed expression of AedaeITP peptide in a number of cells in the brain and in the terminal ganglion. Comparatively, AedaeITP-L peptide was localized solely within the pre-terminal abdominal ganglia of the central nervous system. Interestingly, prolonged desiccation stress caused upregulation of AedaeItp and AedaeItp-l levels in adult mosquitoes, suggesting possible functional roles in water conservation and feeding-related activities. RNAi-mediated knockdown of AedaeItp caused an increase in urine excretion, while knockdown of both AedaeItp and AedaeItp-l reduced blood feeding and egg-laying in females as well as hindered egg viability, suggesting roles in reproductive physiology and behavior. Altogether, this study identifies AedaeITP and AedaeITP-L as key pleiotropic hormones, regulating various critical physiological processes in the disease vector, A. aegypti. [ABSTRACT FROM AUTHOR]

Infraspecific floral trait variations may appear in response to elevational differences in alpine plant species. There is enormous information on the selection of such morphs mediated by biotic and/or abiotic variables. Whether such differences contribute to differences in reproductive strategy and mating outcomes is rarely investigated. We investigated these aspects in two distinct elevational floral morphs (Red and Pink) of Rhododendron arboreum Sm. in Western Himalaya. The red morphs occupy the lower elevations while pink morphs the higher elevations. The two morphs differ in floral traits like phenology, dimension, display, quality of floral rewards, and pollinators that happen to influence interaction with available pollinator pool at each elevation. The pink morph exhibits entomophily, while the red ones show ornithophily. Although experimental pollinations established that both the morphs are selfcompatible, selfing results in significantly lower fruit-set than either cross-or open-pollinations. The outcrossing rate in the red morph, as determined by using simple sequence repeat (SSR) markers, was higher (tm=0.82) than that in the pink morph (tm=0.76), with a tendency of the latter to be shifting towards mixedmating strategy. However, the extent of biparental inbreeding was comparable among the two morphs. It is inferred that the differences in the mating outcomes among the morphs in the tree species are linked to those emerging from floral traits and the pollination by different functional groups of floral visitors. [ABSTRACT FROM AUTHOR]

The history and reproductive biology of the Eupomatiaceae family are reviewed, and the flowering of Eupomatia bennettii is described to highlight the significance of floral parts and their movements for cross-pollination. The highly specialised relationships between Eupomatia spp. and weevil pollinators (Elleschodes spp.) are discussed, and gaps in knowledge of the reproductive biology of Eupomatiaceae are identified. [ABSTRACT FROM AUTHOR]

Orchids are notable for the variety of rewards produced for their pollinators. Nevertheless, many species are rewardless and pollinated by food‐deceptive strategies. This is the case for Cattleya, a Neotropical genus of more than 100 species supposedly pollinated by food‐deception.Here, I studied a member of the clade Sophronitis (Cattleya cernua) in forest areas of southeastern Brazil. The study included analyses of floral morphology, light microscopy of secretory tissues, histochemistry of flower resources, and measurements of nectar volume and concentration. Data on pollinators and mechanisms of pollination were collected in the field by means of film records, while breeding systems were studied using experimental treatments applied to cultivated specimens.The flower traits of Cattleya cernua show strong adaptation to avian pollination. Flowers produce 1–7 μl of a moderately concentrated nectar (17–50%). A secretory tissue at the distal portion of the cuniculus is responsible for nectar secretion. Secreted nectar is stored in the nectar chamber. Pollinaria deposition on the apex of bird beaks allows both short‐ and long‐billed birds to pollinate the flowers. Plants from the studied population were fully self‐compatible but pollinator‐dependent. Pollinator frequency was higher than in food‐deceptive Cattleya.As far as is known, this is the first record of nectar production in Cattleya. The discovery of a reward‐producing species (C. cernua) within a genus entirely pollinated by food‐deception strategies (Cattleya) provides clues and new insights into understanding the diversification of Laeliinae across the Neotropics. [ABSTRACT FROM AUTHOR]

Chelonus inanitus L. (Hymenoptera: Braconidae) is an egg-larval parasitoid of Lepidoptera, with the potential for biological control of noctuids. The lack of information merits research on its reproductive and developmental biology. We studied the reproduction of mated parasitoid pairs during female lifetime and the development of the progeny on two hosts, the factitious Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) and the natural Spodoptera littoralis Boisduval (Lepidoptera: Noctuidae). The longevity of pairs subjected or not to parasitism was compared. Development of the progeny after parasitization and growth rates were studied. Reproduction decreased the longevity of both sexes in every host. The similar survival patterns proved that sex was not a factor influencing longevity. Females parasitized immediately after emergence and offspring production peaked at 2–2.5 days. Low parasitization rates (3–12%) were generally observed. Reproduction on S. littoralis was fourfold less efficient, probably due to failure of immature stages leading to poor progeny. Sex ratio of the progeny was mostly male-biased. Males developed faster in both hosts. Development was 20 days shorter in the natural host. Strikingly, the parasitoid performed successfully better on the factitious host. Inbreeding, poor understanding of host preference, and inadequate rearing and handling practices are discussed as possible causes of the worse parasitoid performance on S. littoralis. [ABSTRACT FROM AUTHOR]

Asteraceae are among the most abundant angiosperm families on oceanic islands. The reproductive biology of Asteraceae is reviewed and the attributes of the family contributing to their success on islands are discussed. Asteraceae are effective dispersers (the small, single-seeded fruits are moved great distances by wind and birds), and colonization is most likely limited by establishment. The pollinators of the colonizing ancestors rarely disperse with them to islands. Divorced from the pollinators of their ancestral habitats, the capitulum of aggregated small, shallow flowers typical of Asteraceae facilitates pollination of colonizers by various novel biotic visitors and by wind. Self-compatible (SC) colonizing ancestors are common, permitting establishment of sexual populations from one or few propagules. However, several large insular lineages of Asteraceae originated from functionally self-incompatible (SI) colonizers that may have also possessed the capacity to set some self-seed. Establishment may also be facilitated by dominance relationships among S-alleles in the sporophytic SI (SSI) system of Asteraceae, increasing cross-compatibility within small populations. Factors potentially promoting outcrossing in SC Asteraceae are: gynomonoecy, with temporal separation of receptive pistillate ray florets and pollen presentation in the hermaphroditic disc florets of a capitulum (interfloral protogyny); and intrafloral protandry where pollen presentation occurs before stigmata become receptive. Dioecy, gynodioecy, and monoecy are infrequent sexual systems in insular Asteraceae. Multiple paternity is similar in island composites to other Asteraceae, suggesting that neither compatible mates nor pollinators are typically limiting. Additional studies, particularly with genomic markers, are needed for more refined insights into mating systems and paternity in island Asteraceae as these results have important conservation implications. [ABSTRACT FROM AUTHOR]

Data on the reproductive biology of elasmobranchs are essential for understanding their life history. Published studies on batoid ray reproductive biology are comparatively scarce, leading to limited understanding and data gaps. The Oceania fantail ray, Taeniura lessoni, is a good example. This Data Deficient nearshore stingray is restricted to Melanesia, with lacking biological and ecological data, including reproduction. To expand upon the limited life-history data for this species, this short paper provides observational data on the reproductive condition in female T. lessoni, at Drawaqa Island, Fiji. Field work involved direct observations and ocean temperature measurements. Over 40 days spanning three months, 105 surveys were conducted across five sites, resulting in 71 sightings of the species. Based on spot patterns and body markings, four female individuals were identified. Between January and March 2024, these females exhibited convex dorsa indicating advanced gestation, transitioning to concave dorsa suggesting parturition. The presence of neonates from early March onwards coincided with the estimated parturition period inferred from the rays' condition. Furthermore, a female previously pregnant was photographed with a dermal abrasion around her pectoral fin, possibly indicating pre-copulatory biting, suggesting a continuous reproductive cycle. The average monthly water temperature at the surveyed sites remained relatively stable throughout the study. Collectively, our findings suggest that Drawaqa Island provides suitable habitat niches for reproductive activities in female T. lessoni. Repeated and long-term data is certainly needed to confirm either a continuous reproductive cycle or seasonal peaks. While preliminary, our observational data represents the first documentation on female reproductive condition in a stingray in Fiji.

Accurate estimate of fetal maturity could provide individualized guidance for delivery of complicated pregnancies. However, current methods are invasive, have low accuracy, and are limited to fetal lung maturation. To identify diagnostic gestational biomarkers, we performed transcriptomic profiling of lung and brain, as well as cell-free RNA from amniotic fluid of preterm and term rhesus macaque fetuses. These data identify potentially new and prior-associated gestational age differences in distinct lung and neuronal cell populations when compared with existing single-cell and bulk RNA-Seq data. Comparative analyses found hundreds of genes coincidently induced in lung and amniotic fluid, along with dozens in brain and amniotic fluid. These data enable creation of computational models that accurately predict lung compliance from amniotic fluid and lung transcriptome of preterm fetuses treated with antenatal corticosteroids. Importantly, antenatal steroids induced off-target gene expression changes in the brain, impinging upon synaptic transmission and neuronal and glial maturation, as this could have long-term consequences on brain development. Cell-free RNA in amniotic fluid may provide a substrate of global fetal maturation markers for personalized management of at-risk pregnancies.

Abstract Tecoma stans is a widely distributed tall ornamental shrub in the plains of Indian subcontinent and is considered an invasive species across Argentina, Australia, South Africa, Pacific Islands and tropical regions of Asia. Besides having an ornamental significance, T. stans has been extensively investigated for its pharmaceutical applications as a source of bioactive compounds. In addition, the shrub is cultivated commercially as a potted flowering plant. We believe that T. stans, being a hardy, invasive and aggressively growing species, holds a considerable potential and a promising solution for re-greening waste and degraded lands outside its invasive range, due to its wider adaptability and drought tolerant characteristics. The shrub is an excellent source of pollen and nectar, that attracts diverse insect-pollinators and several species of birds. The prudent plantation of this shrub has the potential to restore the ecology of barren landscapes, that can change its perspective of ‘being invasive’ to ‘being ecologically healthy’ across the tropical, semi-arid and subtropical regions worldwide. This paper reviews the current updates on ecology, life cycle including morphology, plant growth characteristics, flowering phenology, reproductive biology, breeding system and fruiting of T. stans. In addition, details on insect-pollinator diversity and natural regeneration potential have also been discussed, besides highlighting its therapeutic and landscape use.

[Objective] The torch flower (Colquhounia coccinea) exhibits a typical avian syndrome, and thus has the potential to be developed as a bird-attracting landscape plant. [Methods] With the C. coccinea populations in natural and artificial habitats as the research object, the flowering phenology, flower traits, flower visitor and flower visiting behavior, breeding system, and seed germination traits were studied through observation and experiment, to understand the reproductive traits of C. coccinea and the attraction potential of different flower visitors. [Results] The overall flowering period of C. coccinea lasted for about three months, and the single flowering period was (9.6±0.6) d. Nectar was relatively abundant and dilute, with short corolla tubes and hexose-dominated nectar sugar composition; flower visitors were mainly Apis cerana and a variety of nectarivorous birds, especially short-billed generalist birds, and C. coccinea still attracted bird visitors in artificial habitats. It was completely self-compatble, but pollinators were necessary for pollination, and pollen limitation did not exist. There was no significant difference in seed germination ability among the pollination treatments. A . cerana could pollinate it effectively, and the pollination effectiveness of birds needs to be further verified. [Conclusion] C. coccinea showed a long display time and had an ability to attract birds to visit the flowers. A large number of seedlings can be produced via sexual reproduction.

ABSTRACTApomixis is a mode of asexual reproduction through seed. Apomixis can be classified into two types – gametophytic and sporophytic apomixis. The phenomenon of apomixis in higher plants is limited; however, it seems to play a critical role in the genetic diversity of Garcinia species. Many species in Garcinia undergo apomixis and this review highlights reproductive pathways of various Garcinia species documented over the years. Most of the Garcinia species are endemic. Garcinia species are known for their edible fruits. For the conservation of species and maintenance of genetic diversity, it is necessary to understand the processes by which Garcinia species reproduce.

Tecoma stans is a widely distributed tall ornamental shrub in the plains of Indian subcontinent and is considered an invasive species across Argentina, Australia, South Africa, Pacific Islands and tropical regions of Asia. Besides having an ornamental significance, T. stans has been extensively investigated for its pharmaceutical applications as a source of bioactive compounds. In addition, the shrub is cultivated commercially as a potted flowering plant. We believe that T. stans, being a hardy, invasive and aggressively growing species, holds a considerable potential and a promising solution for re-greening waste and degraded lands outside its invasive range, due to its wider adaptability and drought tolerant characteristics. The shrub is an excellent source of pollen and nectar, that attracts diverse insect-pollinators and several species of birds. The prudent plantation of this shrub has the potential to restore the ecology of barren landscapes, that can change its perspective of 'being invasive' to 'being ecologically healthy' across the tropical, semi-arid and subtropical regions worldwide. This paper reviews the current updates on ecology, life cycle including morphology, plant growth characteristics, flowering phenology, reproductive biology, breeding system and fruiting of T. stans. In addition, details on insect-pollinator diversity and natural regeneration potential have also been discussed, besides highlighting its therapeutic and landscape use. [ABSTRACT FROM AUTHOR]

Background: Most Rubiaceae species are hermaphroditic with outcrossing often promoted by distyly and self-incompatibility, as well as by andromonoecy and dioecy in some species. However, cleistogamy has been reported in three genera, including Richardia. Aims: To characterise the reproductive biology of Richardia brasiliensis (Rubiaceae), a ruderal herb native to southern USA, Central America and large parts of South America, and determine if it exhibits preanthesis cleistogamy. Methods: In three natural populations of R. brasiliensis in Argentina, floral processes were monitored in the flower-bud stage and during anthesis until senescence. In addition, stigma receptivity and pollen viability were determined at these different floral stages in two populations, and experimental controlled pollinations were performed to check for spontaneous self-pollination, natural pollination and apomixis. Floral visiting insects were observed and collected for identification. Results: Autonomous self-pollination occurred when flowers were in bud and stigmas were receptive, with ovules fertilised prior to anthesis. These flowers were protogynous and functionally cleistogamous, though later passed through a chasmogamous phase when flowers opened and were visited by diverse insects. Conclusions: Our study demonstrates that preanthesis cleistogamy occurs in flower-buds of R. brasiliensis, indicating that this mode of self-pollination and self-fertilisation may be more common in Rubiaceae than previously realised. KEY HIGHLIGHTS: Flowers of Richardia brasiliensis exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur. Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds. During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation. [ABSTRACT FROM AUTHOR]

This work aimed to study the reproductive traits of the southernmost landlocked population of Galaxias maculatus living under extreme environmental conditions (54.5° S). Individuals were measured, weighed, sexed, and dissected. The reproductive cycle was analyzed based on the monthly variation of the gonadosomatic indices (GSIs) and the monthly proportion of gonadal stages (established through histology). Fecundity was evaluated by counting macroscopically hydrated oocytes. The reproductive season of G. maculatus in the population assessed extended from March through November (the more protracted for the species described so far). The GSI was higher in males than females, with maximum individual values occurring at the final maturation stage. The simultaneous presence of postovulatory follicles and developing oocytes indicates iteroparity. Our results suggest lower instantaneous investment in reproduction at the individual level but a more prolonged throughout the year than other populations of the species, even under more extreme winter conditions. Considering the increased reproduction costs under extreme habitats and based on our findings, we suggest how populations from the same geographic area can deploy different strategies to cope with energy trade-offs. These results highlight the importance of integrated physiological and ecological approaches to elucidate the basis for variation in life-history traits. [ABSTRACT FROM AUTHOR]

Premise: Understanding the factors that limit reproductive success is a key component of plant biology. Carnivorous plants rely on insects as both nutrient sources and pollinators, providing a unique system for studying the effects of both resource and pollen limitation on plant reproduction. Methods: We conducted a field experiment using wild‐growing Dionaea muscipula J. Ellis (Droseraceae) in which we manipulated prey and pollen in a factorial design and measured flower production, number of fruits, and number of seeds. Because understanding reproduction requires knowledge of a plant species' reproductive and pollination biology, we also examined the pollination system, per‐visit pollinator effectiveness, and pollen‐ovule (P/O) ratio of D. muscipula. Results: Plants that received supplemental prey produced more flowers than control plants. They also had a higher overall fitness estimate (number of flowers × fruit set (total fruits/total flowers) × seeds per fruit), although this benefit was significant only when prey supplementation occurred in the previous growing season. Neither pollen supplementation nor the interaction between pollen and prey supplementation significantly affected overall plant fitness. Conclusions: This study reinforces the reliance of D. muscipula on adequate prey capture for flower, fruit, and seed production and a mobile pollen vector for reproduction, indicating the importance of considering insects as part of an effective conservation management plan for this species. [ABSTRACT FROM AUTHOR]

The results of a study of the ovaries and testes of the Pacific ocean perch Sebastesalutus are presented based on the materials collected in the spring of 2019 and 2021 in the Bering Sea. Histological methods were used to accurately determine the phases of maturity and to visually identify the scale of gonad maturity. The maturity scale includes IX maturity phases for ovaries, and VI phases for testes. Females are characterized by synchronous development of larvae and their hatching; males have multiple-batch spawning. After larvae hatching, the gonads of females are transformed to the developing phase IX–III. According to the physiological state of the gonads, three groups of individuals were identified which differ in size: immature, maturing and mature, taking part in the current year spawning. The maturation of the gonads in the Pacific ocean perch begins at a fork length of 25 cm (FL), 50% of individuals became mature at a fork length of ∼30 cm, and the maturation of all fish occurs at 35 cm or more. [ABSTRACT FROM AUTHOR]

Ovulation is essential for reproductive success, yet the underlying cellular and molecular mechanisms are far from clear. Here, we applied high-resolution spatiotemporal transcriptomics to map out cell type- and ovulation stage-specific molecular programs as function of time during follicle maturation and ovulation in mice. Our analysis revealed dynamic molecular transitions within granulosa cell types that occur in tight coordination with mesenchymal cell proliferation. We identified molecular markers for the emerging cumulus cell fate during the preantral-to- antral transition. We describe transcriptional programs that respond rapidly to ovulation stimulation and those associated with follicle rupture, highlighting the prominent roles of apoptotic and metabolic pathways during the final stages of follicle maturation. We further report stage-specific oocyte-cumulus cell interactions and diverging molecular differentiation in follicles approaching ovulation. Collectively, this study provides insights into the cellular and molecular processes that regulate mouse ovarian follicle maturation and ovulation with important implications for advancing therapeutic strategies in reproductive medicine. [ABSTRACT FROM AUTHOR]