10 results on '"Renner AM"'
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2. Correction: Click functionalized biocompatible gadolinium oxide core-shell nanocarriers for imaging of breast cancer cells.
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Siribbal SM, Ilyas S, Renner AM, Iqbal S, Vázquez SM, Moawia A, Valldor M, Hussain MS, Schomäcker K, and Mathur S
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[This corrects the article DOI: 10.1039/D2RA00347C.]., (This journal is © The Royal Society of Chemistry.)
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- 2022
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3. Click functionalized biocompatible gadolinium oxide core-shell nanocarriers for imaging of breast cancer cells.
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Siribbal SM, Ilyas S, Renner AM, Iqbal S, Muñoz Vázquez S, Moawia A, Valldor M, Hussain MS, Schomäcker K, and Mathur S
- Abstract
Site-specific delivery using functionalized nanocarriers is in high demand in imaging applications of modern clinical research. To improve the imaging capabilities of conventionally used contrast agents and expand the targeting accuracy, functional gadolinium oxide based nanocarriers originated from homogeneous core shells structures (Gd
2 O3 @SiO2 @Fe3 O4 ) were developed using a multilayer formation approach. The synthesis and chemical configuration for the covalent binding of macrocyclic chelating agents and estrogen targeting molecules on these nanocarriers were designed by a two-step chemical synthesis method. Initially, SiO2 @Fe3 O4 structures were prepared and encapsulated with a homogenous thin Gd2 O3 overlayer. The exterior surface of the as-prepared carriers offered chemical binding with a breast cancer specific estrogen molecule, covalently grafted through a Click-Chemistry protocol. In the next step, to enhance the diagnostic imaging capabilities of these carriers, thiocyanate-linked chelator molecule, DOTA, was attached to the surface of estrogen bound Gd2 O3 @SiO2 @Fe3 O4 using basic reaction conditions. The active amino groups before and after conjugation of estrogen molecules on the surface were quantified using a fluorescamine based approach. Due to the covalent binding of the macrocyclic chelator to the Gd2 O3 @SiO2 @Fe3 O4 surface, core shell carriers showed potential radiolabeling efficiency using positron emitter radionuclide, gallium-68 (68 Ga). Intracellular uptake of estrogen-conjugated carriers was evaluated with MCF7 breast cancer cell lines using confocal laser scanning microscopy and fluorescent flow cytometry. In addition, in vitro cytotoxicity studies of functional nanocarriers as compared to bare nanoparticles showed reduced toxicity to HEK-293 cells demonstrating the role of surface attached molecules in preventing direct exposure of the Gd2 O3 surface to the cells. The as-developed gadolinium based nanocarriers presented excellent capabilities as biocompatible target-specific imaging probes which indicates great potential in the field of dual-mode contrast agents., Competing Interests: The authors declare no competing financial conflict of interest., (This journal is © The Royal Society of Chemistry.)- Published
- 2022
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4. High efficiency capture of biomarker miRNA15a for noninvasive diagnosis of malignant kidney tumors.
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Renner AM, Derichsweiler C, Ilyas S, Gessner I, Fries JWU, and Mathur S
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- Biomarkers, Tumor genetics, Humans, Carcinoma, Renal Cell diagnosis, Carcinoma, Renal Cell genetics, Kidney Neoplasms diagnosis, MicroRNAs genetics
- Abstract
To date, there are no preoperative and quantitative dynamics in clinical practice that can reliably differentiate between a benign and malignant renal cell carcinoma (RCC). For monitoring different analytes in body fluids, more than 40 different molecular biomarkers have been identified, however, they are associated with limited clinical sensitivity and/or non-optimal specificity due to their leaky nature. Previous work on RCC demonstrated the miRNA15a to be reliable and novel biomarker with 98.1% specificity and 100% sensitivity. Despite the high potential of miRNA15a biomarker, its clinical application is considerably hampered by the insensitive nature of the detection methods and low concentration of biomarker in samples that is aggravated by the high level of contamination due to other solutes present in body fluids. In this work, a non-invasive quantitative approach is demonstrated to overcome such diagnostics issues through biotin-streptavidin binding and fluorescence active magnetic nanocarriers that ensured prompt isolation, enrichment and purification of the biomarker miRNA15a from urine. The study demonstrates that detectable low levels of these miRNAs through miRNA capturing nanocarriers can potentially function as advanced diagnostic markers for the non-invasive investigation and early detection of renal cancer.
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- 2022
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5. Using an employee resource group to develop GRIT in female healthcare leaders: a case study.
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Seegmiller Renner AM, Borgwardt HL, Coyle M, Moeschler S, and Bhagra A
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- Communication, Female, Health Facilities, Humans, Retrospective Studies, Delivery of Health Care, Leadership
- Abstract
Purpose: This case study aims to demonstrate how the Greater Leadership Opportunities for Women (GLOW) Mayo Clinic Employee Resource Groups (MERG) has positively impacted leadership development focusing on growth, resilience, inspiration and tenacity (GRIT) and increased advancement for female leaders at Mayo Clinic. It will also establish how the innovative utilization of employee resource groups can positively impact the development of leaders within an institution in general and specially can enhance behaviors related to GRIT., Design/methodology/approach: This case study design was used to measure the impact of the GLOW MERG's interventions through qualitative and quantitative approaches that highlight both process and outcome to increase study validity through complementarity, which "seeks elaboration, enhancement, illustration, clarification of the results from one method with the results from another" (Greene, et al. , 1989, p. 259) as well as completeness and context (Onghena et al. , 2019; Schoonenboom and Johnson, 2017; Bryman, 2006). Learning outcomes (knowledge), skill accomplishments and attitude development were evaluated within two weeks after each session and annually through standardized surveys sent to participants via email. The surveys were designed to capture key information about the sessions, including the impact of the session content, the willingness and ability of attendees to apply the learning and identification of opportunities for improvement in session design and delivery, as well as measure satisfaction with the activities offered, the frequency and method(s) of communication, barriers to session attendance and particular topics or speakers of interest to members (Appendix 1). Response options included dichotomous scales, Likert-type scales, multi-select and free text. This provided a voluntary response sampling, as post-session surveys were sent to all session attendees and annual surveys were sent to all GLOW MERG members, which allowed individuals to choose if they would respond to the surveys (Creswell and Creswell, 2018). To foster an environment of continuous improvement, plan-do-study-act (PDSA) cycles (Langley et al. , 2009) were conducted after every survey by the event planning team and the GLOW MERG Board. Interventions were tested, reviewed and discussed during monthly board meetings and event planning. Improvements were made and results were shared with key stakeholders through regular communication channels. Additionally, 30 past and present GLOW MERG leaders were surveyed to measure their perceived impact of participation in the GLOW MERG interventions using dichotomous scales, multi-select and free text responses (Appendix 2). This targeted purposive sample was selected because of their high level of engagement with the MERG to provide a retrospective evaluation of the success of the GLOW MERG, and its interventions for career advancement related to the development of GRIT attributes, knowledge and skills resulting in career advancement for those who are/have been highly engaged with the MERG., Findings: The results spanning the past few years of GLOW MERG interventions has shown that the GLOW MERG has been successful in providing targeted educational events that address the GRIT knowledge, skills and attributes, needed for female health-care leaders to be successful in developing GRIT capabilities. By staying true to its mission and vision, the GLOW MERG has been able to promote, educate and empower female leaders at Mayo Clinic while actively breaking down the barriers that can prevent women from obtaining leadership positions., Research Limitations/implications: There are several limitations with this case study's data collection and sampling methods. First, the post-session and annual survey sampling was based mainly on ease of access, with responses obtained from respondents who are more likely to volunteer or those with the strongest opinions. This allowed for potential bias as responses may not be representative of all GLOW MERG member opinions. Furthermore, the purposive sample of present and past GLOW MERG leaders was also subject to volunteer bias and may not have be representative of the GLOW MERG population. Additionally, the case study examined the practices of only one site and MERG group and may not be representative of all sites or employee resources groups., Practical Implications: The interventions implemented by the GLOW MERG to assist women with developing GRIT knowledge, skills and attributes - barriers women often face in leadership roles - were tested, reviewed and discussed during monthly board meetings and event planning. PDSA cycles were conducted, improvements were made and results were shared with key stakeholders through regular communication channels (Langley et al. , 2009). Key lessons learned from these assessments include: One size does not fit all for leadership development. GLOW members have a wide variety of backgrounds, skills and experiences. Repetition is important in the development of GRIT knowledge, skills and attributes associated with GRIT. A one-time event provides attendees with an information overview and the steps to start developing a new skill but no dedicated time to practice and implement that skill., Originality/value: The innovative utilization of employee resource groups can positively impact the development of leaders within an institution in general and specially can enhance behaviors related to GRIT., (© Emerald Publishing Limited.)
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- 2022
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6. 18 F-Labeled magnetic nanovectors for bimodal cellular imaging.
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Schütz MB, Renner AM, Ilyas S, Lê K, Guliyev M, Krapf P, Neumaier B, and Mathur S
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- Folic Acid, Humans, MCF-7 Cells, Magnetic Resonance Imaging, Magnetics, Spectroscopy, Fourier Transform Infrared, Magnetite Nanoparticles, Nanoparticles
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Surface modification of nanocarriers enables selective attachment to specific molecular targets within a complex biological environment. Besides the enhanced uptake due to specific interactions, the surface ligands can be utilized for radiolabeling applications for bimodal imaging ensured by positron emission topography (PET) and magnetic resonance imaging (MRI) functions in one source. Herein, we describe the surface functionalization of magnetite (Fe
3 O4 ) with folic acid as a target vector. Additionally, the magnetic nanocarriers were conjugated with appropriate ligands for subsequent copper-catalyzed azide-alkyne cycloaddition or carbodiimide coupling reactions to successfully achieve radiolabeling with the PET-emitter18 F. The phase composition (XRD) and size analysis (TEM) confirmed the formation of Fe3 O4 nanoparticles (6.82 nm ± 0.52 nm). The quantification of various surface functionalities was performed by Fourier-transform infrared spectroscopy (FT-IR) and ultraviolet-visible microscopy (UV-Vis). An innovative magnetic-HPLC method was developed in this work for the determination of the radiochemical yield of the18 F-labeled NPs. The as-prepared Fe3 O4 particles demonstrated high radiochemical yields and showed high cellular uptake in a folate receptor overexpressing MCF-7 cell line, validating bimodal imaging chemical design and a magnetic HPLC system. This novel approach, combining folic acid-capped Fe3 O4 nanocarriers as a targeting vector with18 F labeling, is promising to apply this probe for bimodal PET/MR-studies.- Published
- 2021
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7. Receptor-Mediated In Vivo Targeting of Breast Cancer Cells with 17α-Ethynylestradiol-Conjugated Silica-Coated Gold Nanoparticles.
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Renner AM, Ilyas S, Schlößer HA, Szymura A, Roitsch S, Wennhold K, and Mathur S
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- Animals, Cell Line, Tumor, Doxorubicin, Drug Carriers, Drug Liberation, Female, Gold, Humans, Mice, Silicon Dioxide, Breast Neoplasms drug therapy, Metal Nanoparticles, Nanoparticles
- Abstract
Efficient therapies for breast cancer remain elusive because of the lack of strategies for targeted transport and receptor-mediated uptake of synthetic drug molecules by cancer cells. Conjugation of nanoparticles (NPs) with active targeting ligands enabling selective molecular recognition of antigens expressed on the surface of cancer cells is promising for localization and treatment of malignant cells. In this study, covalent attachment of synthetic estrogen 17α-ethynylestradiol on the silica (SiO
2 ) shell of silica-gold NPs (SiO2 @Au) was undertaken to improve the cancer-targeting ability of the nano-biotags. Chemical and structural analysis of the bioconjugates examined in solution (UV-vis and ξ-potential) and solid state (Fourier transform infrared spectroscopy, X-ray diffractometry, and transmission electron microscopy) confirmed the identity of the carrier particles and surface-bound ligands. The mesoporous silica shell served as a reservoir for anticancer drugs (doxorubicin and quercetin) and to facilitate covalent attachment of receptor molecules by click chemistry protocols. The chemoselective recognition between the nanoconjugates and cell membranes was successfully demonstrated by the accumulation of nanoprobes in the tumor tissue of mice with subcutaneous breast cancer, whereas healthy cells were unaffected. The drug release studies showed sustained release kinetics over several weeks. These findings elaborate the exceptional selectivity and potential of estrogen-coated nano-biolabels in efficient diagnosis and detection of breast cancer cells.- Published
- 2020
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8. Executive functions and clinical symptoms in children exposed to maltreatment.
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Carvalho JN, Renner AM, Donat JC, de Moura TC, Fonseca RP, and Kristensen CH
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- Child, Female, Humans, Male, Behavioral Symptoms physiopathology, Child Abuse, Cognitive Dysfunction physiopathology, Executive Function physiology, Inhibition, Psychological
- Abstract
There is evidence of an association between childhood maltreatment and lifelong cognitive impairment. Few studies investigate cognitive functioning in maltreated children. The objective of this study was to investigate whether there are differences in executive processing between maltreated and nonmaltreated children. Additionally, clinical symptoms were compared between groups and possible associations between clinical symptoms, and deficits in executive functions were investigated. The sample consisted of 55 children (8-12 years), 30 with a history of maltreatment and 25 with no history of maltreatment. An interview was conducted with the child's legal guardian and instruments were administered: Juvenile Victimization Questionnaire (JVQ), Child Behavior Checklist (CBCL), and Spence Children's Anxiety Scale (SCAS). The following clinical instruments were administered to the children: JVQ, Trauma Symptom Checklist for Children (TSCC), and Children's Depression Inventory (CDI). Children underwent neuropsychological assessment. Data were analyzed by comparing the groups for clinical and cognitive variables. Differences were found between the groups in all executive functions. Most clinical symptomatology scales showed differences between the groups. Few associations were found between clinical and executive impairment profiles. Cognitive stimulation interventions, focused on inhibitory control, should be proposed in combination with psychotherapy.
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- 2020
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9. Identifying topic sentencehood.
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McCarthy PM, Renner AM, Duncan MG, Duran ND, Lightman EJ, and McNamara DS
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- Humans, Linguistics, Memory, Narration, Cognition, Semantics
- Abstract
Four experiments were conducted to assess two models of topic sentencehood identification: the derived model and the free model. According to the derived model, topic sentences are identified in the context of the paragraph and in terms of how well each sentence in the paragraph captures the paragraph's theme. In contrast, according to the free model, topic sentences can be identified on the basis of sentential features without reference to other sentences in the paragraph (i.e., without context). The results of the experiments suggest that human raters can identify topic sentences both with and without the context of the other sentences in the paragraph. Another goal of this study was to develop computational measures that approximated each of these models. When computational versions were assessed, the results for the free model were promising; however, the derived model results were poor. These results collectively imply that humans' identification of topic sentences in context may rely more heavily on sentential features than on the relationships between sentences in a paragraph.
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- 2008
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10. Fabrication of implant overdentures that are passive and biocompatible.
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Renner AM
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- Biocompatible Materials chemistry, Corrosion, Dental Abutments, Dental Casting Technique instrumentation, Dental Implantation, Endosseous, Dental Soldering, Denture Retention, Electrochemistry, Electrogalvanism, Intraoral, Humans, Male, Metal Ceramic Alloys chemistry, Middle Aged, Patient Care Planning, Stress, Mechanical, Surface Properties, Titanium chemistry, Dental Implants, Dental Prosthesis, Implant-Supported, Denture Design, Denture, Overlay
- Abstract
Passive-fitting mesostructures and superstructures are required to be superimposed on osseointegrated endosseous implants. The tightening of an inaccurate framework to the abutments will transmit stresses to the bone-implant interface. Precision, until now, has been inconsistent and unpredictable with conventional methods of casting, soldering, and welding techniques. By machining the framework with the Spark Erosion method using electrodischarge machining, a substantial improvement in the passive seat of the prosthetic elements is achieved. Chemical reactions caused by galvanic corrosion are also important factors in the precise approach to the treatment planning of implant-supported prostheses. The application of a pure 11.8% grade 1 titanium casting system allows the restorative team to fabricate porcelain-fused-to-metal crowns and bridges, conventional fixed-removable restorations and implant prostheses in pure titanium within completely biocompatible parameters that are absolutely mercury free. This article explains why the spark erosion machining and the unique titanium casting system have a significant impact on today's implant dentistry.
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- 2000
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