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28 results on '"Rasmussen, Akiko"'

1. Mechanosensitive channel gating by delipidation

Author

Flegler, Vanessa Judith, Rasmussen, Akiko, Borbil, Karina, Boten, Lea, Chen, Hsuan-Ai, Deinlein, Hanna, Halang, Julia, Hellmanzik, Kristin, Löffler, Jessica, Schmidt, Vanessa, Makbul, Cihan, Kraft, Christian, Hedrich, Rainer, Rasmussen, Tim, and Böttcher, Bettina

Published
2021

5. The role of lipids in mechanosensation

Author

Pliotas, Christos, Dahl, A Caroline E, Rasmussen, Tim, Mahendran, Kozhinjampara R, Smith, Terry K, Marius, Phedra, Gault, Joseph, Banda, Thandiwe, Rasmussen, Akiko, Miller, Samantha, Robinson, Carol V, Bayley, Hagan, Sansom, Mark S P, Booth, Ian R, and Naismith, James H

Published
2015
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13. The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli

Author

Rasmussen, Akiko, Rasmussen, Tim, Edwards, Michelle D., Schauer, Daniela, Schumann, Ulrike, Miller, Samantha, and Booth, Ian R.

Subjects
Escherichia coli -- Genetic aspects, Mutagenesis -- Usage, Biological sciences, Chemistry
Abstract

Site-directed mutagenesis was used for examining the role of tryptophan (Trp) residues in MscS function. Results suggest that Trp residue W16 plays a significant role in the gating of MscS, whereas Trp residue W240 plays an essential role in MscS oligomer stability.

Published
2007

17. Probing the Structure of the Mechanosensitive Channel of Small Conductance in Lipid Bilayers with Pulsed Electron-Electron Double Resonance

Author

Ward, Richard, primary, Pliotas, Christos, additional, Branigan, Emma, additional, Hacker, Christian, additional, Rasmussen, Akiko, additional, Hagelueken, Gregor, additional, Booth, Ian R., additional, Miller, Samantha, additional, Lucocq, John, additional, Naismith, James H., additional, and Schiemann, Olav, additional

Published
2014
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25. Mechanosensitive Channels: Their Mechanisms and Roles in Preserving Bacterial Ultrastructure During Adaptation to Environmental Changes.

Author

El-Sharoud, Walid, Booth, Ian R., Miller, Samantha, Rasmussen, Akiko, Rasmussen, Tim, and Edwards, Michelle D.

Abstract

The integrity of bacterial cells has long been identified with the possession of the peptidoglycan cell wall. However, the presence of "natural" disruptive forces has been recognized for almost 60 years. Mitchell determined that bacteria possess an outwardly directed turgor pressure of greater than 4 atmospheres. Other early experiments indicated that the substantial pools of amino acids that are retained by bacteria could be released very rapidly by hypoosmotic shock. More recently, two new elements have been added to the equation, one of which is universal and the other may have more limited distribution. The first is the discovery of mechanosensitive channels that open rapidly, producing large holes in the cytoplasmic membrane, in response to increases in membrane tension. The second is that the cell wall is a dynamic structure, in which changes are required during adaptation to stress. The interplay between the two phenomena is examined in this chapter, with much emphasis being placed on the structure and function of mechanosensitive channels. [ABSTRACT FROM AUTHOR]

Published
2008
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26. The Structure of YnaI Implies Structural and Mechanistic Conservation in the MscS Family of Mechanosensitive Channels

Author

Böttcher, Bettina, Prazak, Vojtech, Rasmussen, Akiko, Black, Susan S., and Rasmussen, Tim

Subjects
Structural Biology, protein-lipid interaction, cryo-electron microscopy, fluorescence, osmotic stress, bacteria, Molecular Biology
Abstract

Mechanosensitive channels protect bacteria against lysis caused by a sudden drop in osmolarity in their surroundings. Besides the channel of large conductance (MscL) and small conductance (MscS), Escherichia coli has five additional paralogs of MscS that are functional and widespread in the bacterial kingdom. Here, we present the structure of YnaI by cryo-electron microscopy to a resolution of 13 Å. While the cytosolic vestibule is structurally similar to that in MscS, additional density is seen in the transmembrane (TM) region consistent with the presence of two additional TM helices predicted for YnaI. The location of this density suggests that the extra TM helices are tilted, which could induce local membrane curvature extending the tension-sensing paddles seen in MscS. Off-center lipid-accessible cavities are seen that resemble gaps between the sensor paddles in MscS. The conservation of the tapered shape and the cavities in YnaI suggest a mechanism similar to that of MscS.

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27. Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy.

Author

Pilotas, Christos, Ward, Richard, Branigan, Emma, Rasmussen, Akiko, Hagelueken, Gregor, Huang, Hexian, Black, Susan S., Booth, Ian R., Schiemann, Olav, and Naismith, James H.

Subjects
SPECTRUM analysis, BACTERIAL cells, ION channel gating mechanisms, RESONANCE, OSMOSIS
Abstract

The article presents information on the conformational state of mechanosensitive channels (MscS) in solution identified by pulsed electron-electron double resonance (PELDOR) spectroscopy. MscS gating behaves as safety valves to protect bacterial cells against hypo-osmotic shock by gating in response to intracellular pressure.

Published
2012
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28. Physiological analysis of bacterial mechanosensitive channels.

Author

Booth IR, Edwards MD, Black S, Schumann U, Bartlett W, Rasmussen T, Rasmussen A, and Miller S

Subjects
Ion Channel Gating, Ion Channels genetics, Osmotic Pressure, Bacterial Physiological Phenomena, Ion Channels physiology, Mechanotransduction, Cellular physiology
Abstract

Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.

Published
2007
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