1. Genetic analysis of skull shape variation and morphological integration in the mouse using interspecific recombinant congenic strains between C57BL/6 and mice of the mus spretus species
- Author
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Michel Baylac, Evelyne Heyer, Gaetan Burgio, Xavier Montagutelli, Eco-Anthropologie et Ethnobiologie (EAE), Muséum national d'Histoire naturelle (MNHN)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Génétique Fonctionnelle de la Souris, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Origine, structure et évolution de la biodiversité (OSEB), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), We are very grateful to Isabelle Lanctin for careful breeding of the IRCS, crosses, genotyping, and technical help, Marek Szatanik for genotyping and breeding help, Raphael Cornette and morphometrics platform of the Museum National d'Histoire Naturelle, Paris for skull preparation and technical help, Brendan McMorran for critical reading of the manuscript, and Nicolas Navarro, Christian Klingenberg, and the editor for helpful comments and excellent suggestions on this manuscript to improve its quality., and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,epistasis ,Mus spretus ,Cephalometry ,Congenic ,Quantitative trait locus ,010603 evolutionary biology ,01 natural sciences ,Genetic analysis ,03 medical and health sciences ,Mice ,Pleiotropy ,pleiotropy ,Genetics ,Animals ,Allele ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Recombination, Genetic ,0303 health sciences ,[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] ,biology ,Strain (biology) ,Skull ,fluctuating asymmetry ,biology.organism_classification ,Mice, Inbred C57BL ,Covariation between traits ,quantitative trait loci ,Epistasis ,Hybridization, Genetic ,Procrustes superimposition ,General Agricultural and Biological Sciences - Abstract
International audience; To assess the genetic basis of the skull shape variation and morphological integration in mice, we have used a tool based on the cross between the distantly related mouse species Mus spretus (SEG/Pas strain) and the laboratory strain C57BL/6 called interspecific recombinant congenic strains (IRCSs). The genome of each IRCS consists on average of 1.3% of SEG/Pas derived sequences, located on multiple chromosomes as small-sized, DNA segments. Quantitative trait loci (QTL) on the skull shape, separated into dorsal and ventral sides, were analyzed in 17 IRCSs by a Procrustes superimposition method using 3D landmarks. The shapes of 16 strains differed significantly from C57BL/6. Discrepancy in the QTLs effects was found between the dorsal side and the anterior region of the ventral side due to a differential effect of the SEG/Pas alleles on the skull shape. A comprehensive analysis of all allelic combinations of the BCG-66H strain showed strong epistatic interactions between SEG/Pas segment acting on both skull sides. Epistatic pleiotropy and covariation between sides were dependent in SEG/Pas alleles direction and contributed to the strong morphological integration between sides. Introduction of Mus spretus alleles in a C57BL/6 background induced strong morphological changes mostly in SEG/Pas alleles direction and provided evidence for high level of morphological integration.
- Published
- 2009