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Limited information is available on antimicrobial resistance and biofilm formation of Yersinia pseudotuberculosis. Therefore, the goal of present study was to examine the antimicrobial resistance and biofilm formation of 27 Y. pseudotuberculosis 2/O:3 strains isolated from pork production chain. Antimicrobial resistance was performed with four antimicrobials by the detection of the minimum inhibitory concentrations (MIC). All Y. pseudotuberculosis strains were resistant to erythromycin and sensitive to ciprofloxacin, meanwhile, 37% and 11% of tested bacteria were resistant to tetracycline and streptomycin, respectively. Obtained data on antimicrobial resistance revealed association between Y. pseudotuberculosis isolated from different pig farms and assigned to different genotypes (p < 0.05). All Y. pseudotuberculosis were able to form biofilms. However, no significant differences in biofilm formation of Y. pseudotuberculosis and antimicrobial resistance profile was observed. Additionally, no significant differences in biofilm formation and different bacteria sources, genotypes and farms were observed. Considering the importance of this foodborne pathogen, the data presented is relevant for characterizing Y. pseudotuberculosis as one of human yersiniosis agents. [ABSTRACT FROM AUTHOR]

Copyright of Agricultural Sciences / Zemès ukio Mokslai is the property of Lithuanian Academy of Sciences Publishers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to evaluate the effects of thymol and lactic acid on Campylobacter jejuni in association with the amount of biogenic amines of broiler breast meat during 4 days of storage at +4°C temperature. Treatment with 1% thymol had a significant impact on Campylobacter jejuni counts in broiler meat. The counts of Campylobacter jejuni were reduced by 0.41 log CFU (P ≥ 0.05) and 0.66 log CFU (P ≤ 0.05) on broiler breast meat treated with 0.5% and 1% thymol solution, respectively, compared with an untreated sample contaminated with Campylobacter jejuni. In addition, treatment of broiler meat with 0.5% thymol solution reduced the levels of cadaverine, tyramine and spermine, compared with the control sample (P ≤ 0.05) during 96 hours of storage at +4°C temperature. Besides, the amount of cadaverine and spermine was similarly reduced by 1% thymol solution and 3% lactic acid solution, respectively (P ≤ 0.05). There was no D-lactic acid isomer formed in the samples treated with 0.5% and 1% thymol solution and 3% lactic acid solution (P ≤ 0.05). Therefore, 1% thymol solution could be used not only as the decontamination measure against Campylobacter jejuni but may also reduce the amount of biogenic amines and inhibit the production of D-lactic acid isomer on broiler breast meat. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Science (John Wiley & Sons, Inc.) is the property of John Wiley & Sons, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of the present work was to compare the biofilm formation and resistance of Y. enterocolitica 4/O:3 strains isolated from pig production chain and patients clinical specimens to five antimicrobials (minimum inhibitory concentration). Bacteria with different PFGE genotypes were selected for this study. Eleven Y. enterocolitica 4/0:3 strains from pig production chain and eight strains from patients stool clinical specimens were studied. The results of our study showed that Y. enterocolitica 4/O:3 strains isolated from pig production chain and patients stool clinical specimens shared similar antimicrobial resistance. All Y. enterocolitica strains tested were resistant to ampicillin and erythromycin, yet susceptible to ciprofloxacin. One and two of 19 tested strains were resistant to streptomycin and tetracycline, respectively. No statistically significant differences (P<0.05) between bacteria genotypes and antimicrobial minimum inhibitory concentration were observed. All tested Y. enterocolitica strains formed biofilms, however, no significant difference in biofilm formation was detected between different bacteria genotypes and no correlation between biofilm formation and antimicrobial resistance of different genotypes was observed. [ABSTRACT FROM AUTHOR]

The present study was designed to investigate the occurrence and numbers of Campylobacter spp. on broiler chicken wings and drumsticks at the retails level of the three main poultry meat producers in Lithuania. Samples of chicken wings and drumsticks were collected of each poultry meat producer by visiting randomly selected retail shops once a week from March till October, in 2009. During each visit to a shop, samples of wings and drumsticks were bought without giving a warning. A total of 87 chicken wings and 87 drumsticks samples were collected and tested for Campylobacter spp. Thermophilic Campylobacter spp. were isolated by both direct inoculation on mCCDA selective medium and by selective enrichment in Bolton enrichment broth. Multiplex- PCR method was used for detection and identification of thermophilic Campylobacter species. Our findings showed that overall 46.55% (81 out of 174) of the collected samples were contaminated with campylobacters. Twenty nine samples out of 81 were confirmed as positive only after enrichment procedure. C. jejuni has been found in 69.12% of the tested samples and C. coli in 13.23%, and both species together in 17.65%, respectively. The mean number of Campylobacter bacteria detected on wings at the retail was 1.99 log10 CFU/ml and on drumsticks 2.11 log10 CFU/ml. This study shows high occurrence of broiler meat contamination with Campylobacter spp. at a retail level in Lithuania. Therefore the risk for consumers should be evaluated and an improvement of control measures at poultry production and retail level should be considered to reduce the risk for consumer's infection with Campylobacter spp. [ABSTRACT FROM AUTHOR]

The present study was designed to investigate the prevalence of virulence genes in C. jejuni from different sources. A total of 98 C. jejuni strains from different sources were selected for the study: 34 strains isolated from broiler products (B), of which 17 from fresh poultry and 17 from marinated poultry products; 17 strains from children clinical samples (V); 31 strains from wild birds' faeces (LK), of which 16 from pigeons and 15 from crows; and 16 from dairy cattle faeces (G). Nine virulence genes (cadF, virB11, ceuE, gltA, hcp, cdtA, cdtB, cdtC, flaA) were selected for the study. The identification of the virulence gene was performed by polymerase chain reaction, the visualization of DNA amplicons was done by electrophoresis, and photos were taken in ultraviolet light. The study revealed that the virB11 gene was found only in 6% of C. jejuni strains isolated from dairy cows, while this gene was not detected in Campylobacter strains isolated from other sources. The gltA gene was found in all tested C. jejuni strains. The cdtA virulence gene was prevalent in examined Campylobacter strains as only 3% of wild bird strains lack this gene. The CdtC gene was detected in all C. jejuni strains isolated from the children clinical samples, wild birds and chicken, whereas campylobacters isolated from the cattle did not harbour this gene (19% of all tested strains). The study results revealed that occurrence of virulence genes was differently distributed among strains of C. jejuni and occurrence of certain virulence genes (hcp, cdtC, flaA) depended on the origin of strain isolation. [ABSTRACT FROM AUTHOR]