1. Regarding Bioanalysis Lasting a Few Minutes: Automated Cooling-SPME and Fast-GC for Urinary 2-Phenyl-2-Propanol Monitoring.
- Author
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Dugheri, Stefano, Fanfani, Niccolò, Cappelli, Giovanni, Marigliano, Antonio, Bucaletti, Elisabetta, Squillaci, Donato, Rapi, Ilaria, Venturini, Lorenzo, Pizzella, Giulia, Manetta, Sara, Pavone, Alfonso, Secchi, Michele, Rainaldi, Iacopo, and Mucci, Nicola more...
- Subjects
ACETIC anhydride ,DERIVATIZATION ,CUMENE ,EXPERIMENTAL design ,POLYDIMETHYLSILOXANE - Abstract
An innovative SPME head space GC–MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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