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Tri Rahayu,1,* Yeni Dwi Lestari,1,* Asti Ayudianingrum,1,* Lutfah Rif’ati2 1Ophthalmology Department, Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia; 2National Research and Innovation Agency, Jakarta, Indonesia*These authors contributed equally to this workCorrespondence: Yeni Dwi Lestari, Jl. Kimia No. 8, RT.10/RW.1, Pegangsaan, Kec. Menteng, Jakarta Pusat, Daerah Khusus Ibukota, Jakarta, 10320, Indonesia, Tel +62 2131902885, Email yeni2lestari@yahoo.com; yeni.lestari@ui.ac.idPurpose: Uncorrected refractive errors after cataract surgery contribute to visual impairments. The aim of this study was to investigate the spectacle coverage rate (SCR) following cataract surgery and its relationship with socioeconomic factors in an urban city in Indonesia.Patients and Methods: This population-based cross-sectional study was conducted in 2015 in Jakarta. The former participants of the Rapid Assessment of Avoidable Blindness (RAAB) survey had a history of cataract surgery and met either of the following criteria: (1) wore spectacles with presenting visual acuity (PVA) 6/12 or (2) had PVA less than 6/12 regardless of spectacle use but achieved the best visual acuity (BVA) 6/12 with pinhole correction.Results: Of the 2998 participants of the RAAB survey, 173 (5.6%) (252 eyes) had a history of cataract surgery, among whom 53 (86 eyes) met our inclusion criteria. The SCR was 69.8% and was associated with age group, household income level, education level, and physicians’ recommendation of spectacle wear. Participants who were of nonproductive age (80%), had the highest household income level (88.2%), the highest level of education (87.5%), and had been recommended for spectacle use by their physicians (80.9%) demonstrated higher SCR. Participants with the highest household income had the highest SCR. Patients who had received a physician’s recommendation showed a higher SCR and were 26 times more likely to wear spectacles (odds ratio [OR] 25.99, 95% CI 2.59– 260.10).Conclusion: There is an unmet need for refractive errors after cataract surgery. Factors such as household income levels and physician recommendations were predictive of spectacle wear.Keywords: cataract surgery, RAAB, socioeconomic factors, spectacle coverage rate

Nina Asrini Noor,1 Tri Rahayu,1,2 Tjahjono D Gondhowiardjo1,2 1JEC Eye Hospitals and Clinics, Jakarta, Indonesia; 2Ophthalmology Department, Cipto Mangunkusumo Kirana Hospital, Jakarta, IndonesiaCorrespondence: Nina Asrini NoorJEC Eye Hospital Kedoya, Jalan Terusan Arjuna Utara Nomor 1, Jakarta Barat, DKI, Jakarta 11520, IndonesiaTel +62 813 9520 9670Email nina.noor@jec.co.idPurpose: To describe the prevalence of dry eye disease (DED) and its subtypes in elderly population with cataracts prior to surgery in Jakarta, Indonesia.Patients and Methods: Forty-nine eyes of 49 patients (16 males, 33 females; mean age: 66.6 years) planned for phacoemulsification surgery were consecutively recruited from two tertiary eye hospitals in this hospital-based cross-sectional study. Symptoms were assessed using the Ocular Surface Disease Index (OSDI) questionnaire, and the patient was defined as symptomatic if their OSDI score was ≥ 13. Objective examinations included the noninvasive tear film break up time (NIBUT) and Schirmer tests. The results were deemed abnormal if they were < 10 seconds and < 10 mm for NIBUT and Schirmer, respectively.Results: Participants were classified into four groups: DED (positive symptoms and short NIBUT), preclinical dry eye (positive symptoms without short NIBUT), predisposition to dry eye (short NIBUT without symptoms), and normal group. Median OSDI score was 12.5 (0– 45.0), NIBUT was 10.4 (5.1– 28.9) seconds, and Schirmer score was 8.0 (2.0– 28.0) mm. The prevalence of DED, preclinical dry eye, and predisposition to dry eye were 22.5%, 22.5%, and 26.5%, respectively, while the other 28.5% subjects were normal. The DED group had significantly lower Schirmer test results (4.0 [2– 17] mm) compared to all other groups. Older age (> 70 years) was associated with poorer NIBUT and Schirmer test results and a significant difference between age groups was observed, especially in males.Conclusion: Dry eye is a common problem among the elderly population with cataracts in Indonesia. Almost half of the subjects presented with dry eye symptoms and either categorized as DED or preclinical dry eye, while more than one quarter were predisposed to dry eye. Care must be taken prior to cataract surgery to rule out existing dry eye with additional attention on those presenting without any symptoms as dry eye may develop after surgery.Keywords: dry eye, elderly population, tear film, cataract, cross-sectional study

Three bacterial isolates selected as plant growth-promoting endophytic bacteria (PGPEB) from "Klutuk" banana (Musa balbisiana L.) gained scrutiny for first-time testing on black rice. The presented study pursued an investigation on the impact of endophytic bacteria inoculation obtained from "Klutuk" banana plants on the growth and production traits of black rice. For this research, the three bacterial isolates (K10, K324, and K111) served as inoculums for black rice seeds, testing for synergism. The three isolates inoculation into black rice plants used both single and consortium inoculation methods. With agar media, black rice growth observation began 14 days after plantation (DAP) while on soil, at 30 and 140 DAP with inoculation. Three isolates did not show any antagonistic reactions. Overall, isolate K10 showed less significant improvement in growth and yield traits of black rice compared with two other isolates, i.e., K324 and K111. With agar media, the endophytic bacteria inoculations did not show a significant effect on the growth of black rice, and even isolate K324 was inhibiting in action. However, on soil media, isolate K324 significantly enhanced the number of roots and shoot length in black rice compared with the control at 30 DAP. The single inoculation with isolate K111 has caused increased productivity based on the weight of the root, dry grain weight per clum, plant height, root length, leaf length, and panicle length in black rice. Isolate K324 promotes the growth of black rice on the soil media at 30 DAP, while isolate K111 improves the production traits on the soil media at 140 DAP. The study findings provide a significant basis for the positive impact of endophytic bacterial inoculation on black rice growth and yield traits. [ABSTRACT FROM AUTHOR]

Copyright of Nutrition & Feed Technology Journal / Jurnal Ilmu Nutrisi dan Teknologi Pakan is the property of IPB University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Identification of Globodera rostochiensis was done based on the information given by the Direktorat Perlindungan Hortikultura, that the potato plants in the sub district of Bumiaji, Batu, East Java, were attacked by potato cyst nematode (Globodera). Plants and soil samples were taken from the potato's areas in the sub-district of Bumiaji, especially in the villages of Brakseng (± 1,700-1,800 m a.s.l), Tunggangan (± 1,600-1,700 m a.s.l), Kembangan (± 1,600-1.700 m a.s.l), and Junggo (± 1,200 m a.s.l). Based on morphological observations on eggs, larvae, females, and cysts, the nematode was identified as Globodera rostochiensis with special morphological characters: 1) second stage larvae vermifonn with total body length 531 - 563μ (x 548,4 μ), body width 22 - 26 μ (x 23,6u), stylet well developed with stylet knobs rounded, and part of the posterior portion hyaline in appearance; 2) cyst globular in shape with protruding neck, cyst's length 470 - 1,008 μ (x 638,08 μ), cyst's width 357 - 744 μ (x 490,33μ), and when the vulval basin is lost forming a single circular fenestra. Cuticle surface between anus and vulva basin have more than 12 paralel ridges.

In the past tea plantation at Pagilaran estate is attacked by leaf roller pests. These are Homona coffearia, Cydia leucostoma, and Gracilaria theifora. At present a slug Parmarion pupillaris is found and considered as a new leaf roller pest, because they live inside of the rolled leaf. This symptom is not known before. The population of Parmarion slug is high 3-4 slugs per bush of tea at the sub-division of Pagilaran estate of Pagilaran, Binorong and Garjito. This condition maybe supported by the weather that Pagilaran estate have a high rainfall of 4,842-6,067 mm per year with only 1-2 months of dry season.

The purpose of this research is to analyze resident's adaptation and housing adjustments in accordance with the implementation of the stay at home policy by the Indonesian government during the pandemic. This is a quantitative research with data obtained from respondents using a questionnaire. Data were collected to determine public opinion using a survey method, such as a questionnaire due to the inability to carry out direct and face-to-face interviews during the pandemic. Respondents' opinions were discussed with contingency coefficient analysis and explained descriptively. The result showed that residents adjusted to working and studying from home via online. Most of the adjustments took place in self-owned houses. A significant number agreed to receiving guests and holding community meetings at home by adjusting the terrace and implementing social distancing. Most respondents have adequate awareness of implementing health protocols to limit the spread of COVID19 in their homes, such as by creating space. Residents make adaptations in response to threats to health, welfare, and comfort during the pandemic. Adjustment of housing space is carried out for awareness and the existence of a health protocol that needs to be met. The more complex the requirements, the more varied the adjustments. [ABSTRACT FROM AUTHOR]

The International Classification of Function, Disability, and Health (ICF-WHO, 2001) recognizes several dimensions of disability, such as body structure and function (and impairment thereof), activity (and activity restrictions) and participation (and participation restriction) and their interactions with contextual factor (personal and environmental). In this study, we map and analyse the relationship between the components of ICF in leprosy patients from two rural areas in Indonesia: Lewoleba (East Nusa Tenggara) and Likupang (North Minahasa). This study was part of a community outreach program by the KATAMATAKU team from Universitas Indonesia. The body structure was graded using the WHO hand and feet disability grade and the number of enlarged nerves, while the body function was measured by the Jebsen Taylor Hand Function Test (JTT) and Timed-up and Go (TUG). Activity limitation and participation restriction were measured using the Screening Activity Limitation Safety Awareness (SALSA) Scale and Participation Scale (P-scale), respectively. There were 177 leprosy patients from the two regions and 150 patients with complete data were included in the analysis. We found 82% (95% CI: 75.08%-87.32%) of subjects with multibacillary leprosy, 10.67% (95% CI: 6.67%-16.62%) of subjects with grade 2 WHO hand disability, and 9.33% (95% CI: 5.64%-15.06%) of subjects with grade 2 WHO foot disability. Assessment using the SALSA Scale showed 29.33% of subjects with limitation activity and 11.33% with participation restriction. Age was shown to have positive correlations with SALSA, JTT, and TUG. Inter-dimensional analysis showed that the SALSA scale had significant positive correlations with the number of nerve enlargements, P-scale, JTT, and TUG. SALSA scores of grade 2 WHO hand and foot disability were also significantly higher than grades 1 and 0. The participation scale also had a positive correlation with JTT but not TUG. Hand disability seemed to affect societal participation while foot did not. We used the ICF to describe and analyse dimensions of leprosy-related disability in Indonesia., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Wahyuni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Cemetery soils most likely contain degradative bacteria which possibly have beneficial potencies. However, the bacterial exploration in these potencies is still limitedly conducted in Indonesia. The raw sequence data of total bacteria in the cemetery soils through metagenomic analysis have been revealed. The data were obtained by collecting soil samples from six spots of two major Cemetery areas, which were Pracimaloyo (P) and Bonoloyo (B), in Surakarta City, Central Java, Indonesia. The six sample spots consisted of two samples from P area with respectively 20 cm and 140 cm depths and four samples of each two samples from B area with 20 and 40 cm depths. The total DNA was subsequently extracted from the collected soils using ZymoBIOMICS DNA Miniprep Kit. The total DNA then was amplified using a couple of 16S rRNA primers through Illumina HiSeq 2500 PE250 (Novogen, Korea) environment system. The raw sequence data has been submitted to the National Center for Biotechnology Information (NCBI) with project ID PRJNA997385. The archived sequence can be accessed in the NCBI website with the following URLs https://www.ncbi.nlm.nih.gov/sra/PRJNA997385. A brief analysis of the sequence data showed that the most common phyla in 20 cm-depths were Proteobacteria (29.5%), Actinobacteria (21.6%), and Firmicutes (19.2%), while Actinobacteria were the most found in 140 cm-depths with 34.2% followed by Proteobacteria (21.9%) and Firmicutes (16.6%). This data would be the first report of total bacterial sequence from cemetery soils in Indonesia., (© 2023 The Author(s).)

Genetically modified MON 89034 corn ( Zea mays L.) expressing Bacillus thuringiensis ( Bt ) insecticidal proteins, viz. Cry1A.105 and Cry2Ab2, is a biotechnological option being considered for the management of the major corn pest in Indonesia, the Asian corn borer ( Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)). As a part of a proactive resistance-management program for MON 89034 corn in Indonesia, we assessed the baseline susceptibility of field-collected populations of O. furnacalis to Cry1A.105 and Cry2Ab2 proteins. Dose-response bioassays using the diet-dipping method indicated that the lethal concentration (LC 50 ) values of Cry1A.105 and Cry2Ab2 in 24 different field populations of O. furnacalis ranged from 0.006 to 0.401 µg/mL and from 0.044 to 4.490 µg/mL, respectively, while the LC 95 values ranged from 0.069 to 15.233 µg/mL for Cry1A.105 and from 3.320 to 277.584 µg/mL for Cry2Ab2. The relative resistance ratios comparing the most tolerant field populations and an unselected laboratory population were 6.0 for Cry1A.105 and 2.0 for Cry2Ab2 based on their LC 50 values. Some field populations were more susceptible to both proteins than the unselected laboratory population. The LC 99 and its 95% fiducial limits across the field populations were calculated and proposed as candidate diagnostic concentrations. These data provide a basis for resistance monitoring in Bt Corn and further support building resistance-management strategies in Indonesia.

Background: People with leprosy who have been declared Release From Treatment (RFT) are often not aware of the leprosy sequelae possibility which can decrease their quality of life. This could be because they have been adapting for a long time hence they do not feel the need to see physicians. This study seeks to compare the results of Vision-Related Quality of Life (VR-QoL) among RFT persons based on the National Eye Institute Visual Functioning Questionnaire-25 (NEI-VFQ-25) and WHO grading disability based on physical examination., Methods: A cross-sectional study of 325 RFT subjects from leprosy communities (Singkawang, West Kalimantan and Tangerang, Banten) was conducted between 2018 and 2019. We used the NEI-VFQ-25 questionnaire that had been validated and translated into Indonesian and distributed to the leprosy population. Relationships and comparisons among variables were evaluated using Kruskal-Wallis and Mann-Whitney tests., Results: There were three main results: The median composite score of VR-QoL for WHO grade 0, 1, and 2 disabilities has decreased by 13%, 25.5%, and 30% of the maximum value, respectively. Of the total, eleven subscales were statistically significant between WHO grading disability and VR-QoL based on the NEI-VFQ-25 (p < 0.05). The comparison between grade 0 and grade 2 disability in all subscales was statistically significant (p < 0.05)., Conclusions: The grade of disability is related to their VR-QoL assessment using the NEI-VFQ-25 questionnaire. Thus, it can be used as an initial screening in primary healthcare settings to increase awareness of disability before a thorough physical examination., (© 2023. BioMed Central Ltd., part of Springer Nature.)

Global food production is anticipated to rise along with the growth of the global population. As a result, creative solutions must be devised to ensure that everyone has access to nutritious, affordable, and safe food. Consequently, including insects in diets has the potential to improve global food and nutrition security. This paper aims to share recent findings by covering edible termites as the main aspect, from their consumption record until consumer acceptance. A total of 53 termite species are reported as edible ones and distributed in 6 biogeographic realms. Generally, termites have a nutrient composition that is suitable for human consumption, and cooked termites are a better dietary choice than their raw counterparts. Besides, increasing customer interest in eating termite-based food can be achieved by making it more palatable and tastier through various cooking processes, that is, boiling, frying, grilling, roasting, smoking, and sun-drying. Moreover, edible termites can also be used as a new source of medication by exhibiting antimicrobial activity. Regarding their advantages, it is strongly encouraged to implement a seminatural rearing system to sustain the supply of edible termites. Overall, this paper makes it evident that termites are an important natural resource for food or medicine. Hence, the long-term objective is to stimulate scientific inquiry into the potential of edible insects as an answer to the problem of global food security., (© 2023 Institute of Food Technologists®.)

Black soldier fly (BSF), Hermetia illucens (L.) (Diptera: Stratiomyidae), is predominantly reared on organic wastes and other unused complementary substrates. However, BSF may have a buildup of undesired substances in their body. The contamination of undesired substance, e.g., heavy metals, mycotoxins, and pesticides, in BSF mainly occurred during the feeding process in the larval stage. Yet, the pattern of accumulated contaminants in the bodies of BSF larvae (BSFL) is varied distinctively depending on the diets as well as the contaminant types and concentrations. Heavy metals, including cadmium, copper, arsenic, and lead, were reported to have accumulated in BSFL. In most cases, the cadmium, arsenic, and lead concentration in BSFL exceeded the recommended standard for heavy metals occurring in feed and food. Following the results concerning the accumulation of the undesired substance in BSFL's body, they did not affect the biological parameters of BSFL, unless the amounts of heavy metals in their diets are highly exceeding their thresholds. Meanwhile, a study on the fate of pesticides and mycotoxins in BSFL indicates that no bioaccumulation was detected for any of the target substances. In addition, dioxins, PCBs, PAHs, and pharmaceuticals did not accumulate in BSFL in the few existing studies. However, future studies are needed to assess the long-term effects of the aforementioned undesired substances on the demographic traits of BSF and to develop appropriate waste management technology. Since the end products of BSFL that are contaminated pose a threat to both human and animal health, their nutrition and production process must be well managed to create end products with a low contamination level to achieve a closed food cycle of BSF as animal feed., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)