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More aggressive reservoir conditions, such as higher concentrations of CO2 corrosive gases, have a massive effect on corrosion. Moreover, additional aqueous species, such as organic acids, have complicated the conditions and enhanced corrosion to the maximum rate. This study investigates and screens three commercial corrosion inhibitors on their performance in mitigating corrosion at welded areas of carbon steel in high CO2 conditions with the presence of organic acids. These investigations were conducted via chemical elucidations to study the functional groups present in the compounds that affect the inhibition performance and the electrochemical measurements to evaluate the inhibition activities and efficiency of these commercial inhibitors under extreme conditions of high CO2 and organic acids. The results indicated that only CT5727 and CT17906 gave a high inhibition efficiency of 90% in the absence and presence of organic acid, with CT5727 giving the lowest corrosion rate of 0.038 mmpy in the absence of organic acid, while CT17906 gave the lowest corrosion rate of 1.00 mmpy in organic acid condition. On the other hand, D4305 gave the worst inhibition performance under both conditions. The elucidation of these inhibitors also found that specific functional groups such as hydroxy, carboxylic acid, alkyl and alkene presence in the structure were believed to affect the inhibition performance of these commercial inhibitors in mitigating corrosion. These could be used as a future reference for the new development of corrosion inhibitors in similar conditions.

This study reports the outcomes of a survey on the use of additives in 120 Egyptian grow-out farms carried out between 2018 and 2019. The survey focused on farms rearing Nile tilapia (Oreochromis niloticus) in the biggest tilapia farming region in Egypt (Kafr El-Sheikh). Results were analysed to explore whether any type of additive was used, whether they were feed- or water-based and the frequency of use. A range of farm characteristics and farm management practices were used as independent variables to explain observed additive use patterns reported by farmers. The survey also gathered production data to explore a potential relationship between the use of additives and total marketable yield or mortality. The results of this survey display very low use of any sort of additive in this tilapia farming region (

Nuestro estudio se concentra sobre una comparación práctica entre un grupo de oraciones en español y su traducción a nuestra lengua árabe para facilitar en entendimiento del modo subjuntivo en español y al mismo tiempo explicar lo que equivale en árabe. Tanto el subjuntivo en español como el aoristo nasbado en árabe requieren unas ciertas condiciones y reglas para que sean en este modo verbal, por lo tanto, hemos tratado explicar estos motivos en cada oración con una explicación si sucede la correspondencia modal o no entre cada par de verbos comparados de las oraciones de dichas lenguas. Al mismo tiempo en muchas ocasiones no sucede esta correspondencia por un motivo u otro, ya que no cada verbo en subjuntivo al traducirlo al árabe dentro de la oración tiene que ser en imperfectivo de subjuntivo árabe. Palabras clave : Subjuntivo, indicativo, yusivo, correspondencia

This study was aimed to investigate the synergistic effects of selenium (Se-NP) and zinc oxide (ZnO-NP) nanoparticles on growth performance, hemato-biochemical profile, immune and oxidative stress responses, and intestinal morphometry of Nile tilapia (Oreochromis niloticus). Monosex Nile tilapia (12.50 ± 1.03 g, N= 180) were randomly allocated into 4 groups in triplicates. Fish were fed diet supplemented with 0 Se-NP and Zn-NP (control group, CG), while fish in the other experimental groups were fed diet supplemented with 1 mg/kg diet Se-NP (Se-NP group), 10 mg/kg diet ZnO-NP (Zn-NP group), and a mixture of 1 and 10 mg/kg diet Se-NP and Zn-NP, respectively (Se/Zn-NP group) for 60 days. Fish fed diet containing Se-NP, Zn-NP, and Se/Zn-NP showed higher final body weight, weight gain, weight gain rate, specific growth rate, and lower feed conversion ratio with respect to CG (P

This study contributes data on haematological and biochemical parameters of African catfish, Clarias gariepinus. It employed a 3 ? 2 factorial design with three ponds of different water depth (0.5, 1 and 1.5 m) and two types of feed (floating and sinking). Twelve earthen ponds (1 m x 2 m) were stocked with 16 fingerlings catfish each (mean weight ~100g) and their blood parameters were monitored over 12 weeks. Differences in hematological parameters related to water depth were mostly significant, and better results were recorded in fish reared in shallower water ponds. Feed type showed improved hematological parameters with using of sinking diet. Most biochemical parameters showed significant differences in pond waters depth and feed type with better results coincided with rearing fish in shallower water depth and with sinking feed. Conclusively, culturing Catfish in shallow ponds (0.5 m) and use of sinking feed improve physiological response and health condition.

Isolates of Streptococcus iniae were recovered from the diseased fish during summer season, identified and characterized using different laboratory techniques. Three hundred and sixty Nile tilapia (100 ± 5.0 g average weight) were divided into two subgroups in triplicates with 20 fish per replicate and received either sterile PBS (B0, B1 and B2, respectively) or S. iniae (B0+ S. iniae, B1+ S. iniae and B2 S. iniae, respectively). At 15 days post‐challenge, results showed low mortality rates in probiotic‐fed groups. The mortalities ranged from 6.67% in B2 to 81.67% in B0 following S. iniae challenge. The haematological parameters showed a significant increase (p < 0.05) in haemoglobin, red blood cells and white blood cells in B1, B1+S. iniae, B2 and B2+ S. iniae groups compared to B0+ S. iniae group. The alanine aminotransferase (ALT) level was elevated (p < 0.05) in tilapia challenged with S. iniae compared to the control groups. Glucose and creatinine levels were elevated (p < 0.05) in all groups compared with B0. Both albumin and globulin levels were significantly low (p < 0.05) in B0+ S. iniae compared to other groups. The total protein level, phagocytosis and phagocytic index were significantly lower (p < 0.05) in B0+ S. iniae than other groups. Histological analysis showed that the hepatopancreas of B0, B1 and B2 groups represents normal hepatocyte architecture, while the infected tilapia showed severe diffused necrosis, mononuclear cell infiltration and loss of the normal architecture. Spleen of PBS control groups did not show any pathological changes, while that of infected tilapia revealed several necrotic areas in addition to marked depletion of lymphoid cells. A loss of skin layers was observed in infected tilapia with marked interstitial oedema and leucocytic cell infiltration which was not observed in the probiotic‐fed fish. The current study highlights the immunomodulatory effect of Bacillus probiotic mixture against S. iniae infection in tilapia that can be further applied to control the disease in farms.

This study was aimed to evaluate the impact of different monochromatic LED light colour (white ‘WL’, green ‘GL’, blue ‘BL’ and mixed green and blue ‘GL × BL’) and two levels of light intensity (5 lx and 20 lx) on growth performance, physiological responses (haematological and biochemical), behaviour and fear reactions in broiler. A 4 × 2 factorial treatment structure was used to evaluate the impact of 4 LED light colours (WL, GL, BL and GL × BL) and 2 levels of light intensity (5 lx and 20 lx) in triplicates. One-day-old Cobb Avian-48 chicks (N = 384, 36.29 ± 0.092 g) were used. Results revealed a significant effect of light colour on performance (final weight, weight gain and feed conversion ratio [FCR]), haematological parameters (haemoglobin, RBCs, PCV, TLC and H/L ratio), biochemical parameters (TP, albumin (AL), and AL/globulin (GL) ratio), tonic immobility (TI) duration and open field (OF) test (latency to first step, mobility duration, immobility duration, immobility sitting, immobility standing, pecking and dropping) with the best results observed in GL × BL. Light intensity had no effects on studied parameters except for H/L ratio, TI duration, latency to first step, immobility sitting, immobility standing and pecking with better findings detected in 5 lx. There was a significant light colour × light intensity interaction on final weight, weight gain, AL, TI duration, mobility duration, immobility duration, immobility sitting, immobility standing and pecking. Conclusively, monochromatic LED light (GL × BL, BL and GL) particularly of low intensity could be a source of light in broiler houses that might have the potential to improve broiler welfare.Highlights LED light is an important factor in the broiler production. Mixed blue and green, blue or green light of 5 lx improves broiler performance and health status. Mixed blue and green, blue or green light of low intensity reduced fear reaction in broilers.

Effects of dietary supplementation of Tribulus terrestris (TT) and 17α‐methyl testosterone (MT) on growth performance and reproductive efficiency of male Nile tilapia, Oreochromis niloticus (N = 75, initial weight 60 ± 3.251 g), were evaluated. Five experimental treatments in triplicate were fed on basal diet (30% crude protein) (control group, CG), basal diet containing 60 mg MT (MT group) and basal diet containing 250, 500 and 750 mg/kg diet TT (TT250, TT500 and TT750 groups), respectively, for consecutive 45 days. Results revealed that the fish received TT in diet showed better growth performance (final body weight, weight gain, feed conversion ratio, specific growth rate, testes weight and gonadosomatic index) compared with MT and CG (p

Effects of dietary supplementation of Bifidobacterium longhum, Bifidobacterium thermophilum, Bacillus subtilis, and Lactobacillus acidophilus on growth performance, health condition, body chemical ...

Zinc is an essential element for metabolism of Nile tilapia (Oreochromis niloticus). Nanomaterials have important benefits in aquaculture. The present study evaluated the effects of green-synthesized zinc oxide nanoparticles (ZnO-NPs) using Ulva fasciata extract as an anti-fungal agent against Candida albicans (C. albicans) in vitro and in vivo in O. niloticus. A total of 252 apparent healthy O. niloticus (20 ± 0.457 g/fish) were randomly allocated into six groups: The 1

This study was aimed to evaluate the efficiency of Sargassumpolycystum and nucleotides- supplemented diets to improve immune response and cold-tolerance of juvenile Litopenaeus vannamei. Four treatments were evaluated: T1, the control, shrimp received only a basal diet, T2, a basal diet with 500 ppm nucleotides, T3, a basal diet with 500 ppm S.polycystum powdered, T4, a basal diet with 500 ppm nucleotides and 500 ppm S.polycystum powdered. Shrimp were fed experimental diets for 56 days. Results revealed shrimp fed T4 diet exhibited the best significant improvement in water quality, survival, growth, and feed utilization indices followed by T2, and T3, while T1 showed the worst values. Additionally, nonspecific immune responses (phagocytosis (%), lysozyme, phenoloxidase, super oxide dismutase (SOD) activity, total nitric oxide) were improved with 1.7–3.2-fold in T4 higher than T1. Histomorphology of hepatopancreas in T4 showed the most increased activation of the hepatic glandular duct system compared with the other treatments. Moreover, nucleotides/seaweed-supplemented diets upregulated relative expression of cMnSOD, Penaeidin4, and heat shock protein70 (HSP70) genes, while translationally controlled tumor protein (TCTP) was downregulated. In conclusion, the synergistic effects of both S. polycystum and nucleotides have many advantages as a growth promoter, immunostimulant, antimicrobial, and cold-tolerant stimulant to L. vannamei.

This study was aimed to investigate the synergistic effects of selenium (Se-NP) and zinc oxide (ZnO-NP) nanoparticles on growth performance, hemato-biochemical profile, immune and oxidative stress responses, and intestinal morphometry of Nile tilapia (Oreochromis niloticus). Monosex Nile tilapia (12.50 ± 1.03 g, N= 180) were randomly allocated into 4 groups in triplicates. Fish were fed diet supplemented with 0 Se-NP and Zn-NP (control group, CG), while fish in the other experimental groups were fed diet supplemented with 1 mg/kg diet Se-NP (Se-NP group), 10 mg/kg diet ZnO-NP (Zn-NP group), and a mixture of 1 and 10 mg/kg diet Se-NP and Zn-NP, respectively (Se/Zn-NP group) for 60 days. Fish fed diet containing Se-NP, Zn-NP, and Se/Zn-NP showed higher final body weight, weight gain, weight gain rate, specific growth rate, and lower feed conversion ratio with respect to CG (P0.05) with the highest being in fish fed with Se/Zn-NP. Fish fed with Se/Zn-NP showed higher hemoglobin, red blood cells, and globulin (P0.05). The highest phagocytic activity, phagocytic index, lysozyme activity, and immunoglobulin M was recorded in fish that received Se/Zn-NP followed by Se-NP, Zn-NP, and the lowest in CG (P0.05). Fish that received diet supplemented with Se-NP, Zn-NP, and Se/Zn-NP significantly (P0.05) increased superoxide dismutase and catalase while reduced malonaldehyde activity compared to CG. Intestinal morphometry revealed significantly (P0.05) increased villi length and goblet cells number in fish fed with Se-NP and/or Zn-NP. In conclusion, dietary supplementation of Nile tilapia with Se-NP and Zn-NP induces synergistic effects that improve growth performance, blood health, and intestinal histomorphology.

The remediation of wastewater (WW) is a promising solution for limited water sources. This study aimed to evaluate rice straw (RS) and zeolite (Z) as bioadsorbents for the removal of pollutants, including heavy metals (HMs) (cadmium [Cd], nickel [Ni], and lead [Pb]) and malathion (PC), from WW and to assess the suitability of reusing remediated WW in fish rearing units. A total of 11 treatment groups with 3 replicates each were designed with different combinations of RS and/or Z for the treatment of real WW contaminated with HMs and malathion, where the WW remained in contact with the adsorbents for 24 h. Different remediated WWs were used for rearing Nile tilapia (Oreochromis niloticus), which were randomly allocated into 33 glass aquaria representing 11 treatments with 3 replicates each for 30 days. The best remediation efficiency was achieved using a mixture of whole RS (WRS), chopped RS (CRS), and Z (HM-PC-WRS-CRS-Z group), with removal percentages of 92%, 95%, 96%, and 99% for Cd, Ni, Pb, and malathion, respectively. The health status of the aquatic ecosystems was assessed through blood tests to characterize biochemical parameters and through pathological changes of cultured O. niloticus reared in treated WW. A significant (P ˂ 0.05) effect on the blood biochemistry of fish reared in treated WW was found and better biochemical and histologic architecture was observed than that of fish reared in untreated WW. A novel mixture of WRS, CRS, and Z could possibly be a promising low-cost adsorbent for wastewater treatment. Graphical abstract.

The current study aimed to evaluate whether clenbuterol, a β2-adrenergic agonist, supplementation in Nile tilapia (Oreochromis niloticus) diets can influence growth and blood parameters. Besides, assessment of adipogenic genes as fatty acid synthase (FAS) and lipoprotein lipase (LPL) which is a key enzyme in the regulation of the flux of fatty acids in liver, muscle, and adipose tissue as well as muscle growth-regulating genes as myostatin (MYO) in muscle and insulin-like growth factor-1 (IGF-1) in liver. The fish were allocated into three equal groups; control group that fed basal diet only and the other two groups fed a basal diet containing clenbuterol at two doses 5 ppm and 10 ppm/kg diet for 30 consecutive days. Results revealed that clenbuterol supplementation significantly increased body weight, decreased liver, spleen and abdominal fat weights, and decreased total circulatory cholesterol and triacylglycerol levels. Moreover, clenbuterol inhibits lipogenesis by downregulation of FAS gene expression by dose and time-dependent manner in the liver while enhanced lipolysis in both the liver and in the adipose tissue. Moreover, lipolysis was reduced in muscle by dose 10 ppm on day 30. Furthermore, clenbuterol presented higher gene expression of MYO and IGF-1 in muscle and liver respectively by dose 5 ppm at day 15 on the other hand, these findings were reversed by day 30 compared with control. In conclusion, clenbuterol efficacy was apparent in a dose and time response pattern to boost growth and reduce fat deposition rates, indicating for the first time that clenbuterol has a profitable growth impact on Nile tilapia.

African catfish (Clarias gariepinus) has been growing in popularity as a culture species globally. Although considerable research has been carried out on this species’ feeding preferences, no research has been carried out on optimum water level for growth. This is particularly surprising given this species' benthic nature. This study assessed the effect of different combinations of water levels and types of feed on the growth performance of African catfish. The study employed a 3 × 2 factorial design with three ponds of different water levels (0.5, 1 and 1.5 m) and two types of feeds (floating and sinking). Twelve earthen ponds (1 × 2 m) were each stocked with 16 catfish fingerlings (mean weight ~100 g), and their growth was monitored for 12 weeks. The fish cultured in the shallowest water grew significantly faster than those cultured in the deepest ponds. At the same time, fish cultured in the shallowest ponds had the lowest feed intake rates and consequently the lowest feed conversion ratios. Catfish fed sinking pellets grew faster than those fed floating pellets although the difference was not significant. There was no significant interaction between pond water depth and feed type. The results of this study suggest that the growth and feeding efficiency of C. gariepinus can be optimized by culturing in fairly shallow ponds (0.5 m).

The present investigation aimed to evaluate the influence of selenium nanoparticles (Se-NPs) or/and spirulina (SP) on the growth, immunity, and oxidation resistance of Nile tilapia. Four groups of fish fed diets with Se-NPs or/and SP at 0 g (control), 1 g SP/kg diet (SP), 1 mg Se-NPs/kg diet (Se-NPs), and 1 g SP + 1 mg Se-NPs/kg diet (SP/Se-NPs) for 60 days. Fish fed Se-NPs or/and SP displayed significantly improved weight gain (WG) and decreased feed conversion ratio (P 0.05). The highest WG has observed in fish fed both Se-NPs and SP, while the specific growth rate was improved by feeding Se-NPs only or both Se-NPs and SP (P 0.05). Blood albumin was increased significantly with Se-NPs with regard to the control (P 0.05), while there were no significant differences between fish fed Se-NPs or/and SP. Blood total protein also was improved by feeding Se-NPs only or both Se-NPs and SP (P 0.05). Further, blood immunoglobulin M was increased by feeding both Se-NPs and SP (P 0.05), while the differences were insignificantly differing with fish fed only Se-NPs (P 0.05). The transcription of liver superoxide dismutase (SOD) and tumor necrosis factor-alpha (TNF-α) genes was upregulated significantly by Se-NPs or/and SP (P 0.05). Interestingly, TNF-α was significantly upregulated by SP when compared to those fed Se-NPs only or both Se-NPs and SP. However, heat shock protein 70 (HSP70) gene transcription was downregulated by Se-NPs or/and SP (P 0.05). Based on the measured parameters, the mixture of both Se-NPs and SP is highly recommended for the welfare of Nile tilapia.

This experiment was carried out to determine the effect of blindfold and tail bend during restraint of Egyptian buffaloes on behavioural reactivity and physiological responses to stressful handling procedures. Twenty-four buffalo bulls, naïve to the testing situation, were arbitrarily assigned to either blindfold (visual restriction) (BF), tail bending (physical control) (TB) or control (CT) (no visual restriction or physical control) treatment during restraint. For three minutes each, during veterinary procedures animals entered the squeeze chute and were subjected to testing trials. Heart rate (HR), respiratory rate (RR) and various behaviours of reactivity were recorded. Average HR and RR decreased in both TB and BF bulls but the reduction was greater in BF bulls. Use of the blindfold and tail bend decreased behavioural indicators of reactivity including: chest chute forcing, head move, kicking and struggling. The reduction was greater in BF bulls in the case of use of the chest chute and struggling. Both tail bend and blindfolded buffaloes decreased behavioural and physiological indicators of stress but BF appeared more beneficial, and may therefore be recommended to reduce stress accompanying routine veterinary examination of buffaloes. Keywords: Buffalo, Blindfold, Tail bend, Restraint, Welfare

This study was aimed to explore differences between day and night feeding of farmed European eels ( Anguilla anguilla ) on: (1) the growth and feeding behaviour of eels; and (2) the subsequent impact on the quality of the culture water. A 30 day field experiment was conducted in which 40 European eels (186.4 ± 9.26 g) were arbitrarily allocated into two replicate concrete ponds (200 x 100 x 125cm) that were divided into two treatment groups, one receiving feed by day (DF) and the other receiving feed at night (NF). Results revealed a significant improvement in eel's growth performance (larger final weight and faster growth) of the fish that were fed by night (NF). Moreover, feeding behaviour was also improved in the NF group with a significantly shorter latency to start feeding and shorter feeding duration. As a consequence of improved feeding behaviour, more favourable water quality conditions (higher dissolved oxygen concentrations and lower ammonia concentrations) were observed in the NF group. In conclusion, feeding eels by night could considerably enhance the culturing of eels by promoting enhanced growth and feeding behavior, and simultaneously improving water quality. Key words : feeding regime; European eel; behaviour; nocturnal feeding; growth; performance Â

This experiment aimed to evaluate the toxic influence of ochratoxin- A (OTA) feed contamination and the effectiveness of some feed-additives (Humic acids, Bio-Plus 2B, Bio-Mos) in detoxifying ochratoxicosis. Thirty five day old weaned Animal Production Research Institute (APRI) rabbits were selected and were allocated to four groups. Group 1 (control, OTA group): rabbits were fed basal diet (ochratoxin- A level: 19 I¼g /kg diet). Group 2: rabbits were fed a basal diet containing 0.2 % humic acid. Group 3: rabbits were fed a basal diet containing 0.4 mg/kg diet Bacillus subtilis and Bacillus licheniformis (Bio-Plus 2B). Group 4: rabbits were fed a basal diet containing 0.1% Manna oligosaccharides (Bio-Mos). The control group showed significantly reduced feed intake, daily weight gain and growth performance index. Furthermore, there was a significant increase in AST, ALT, malondialdehyde, creatinine, and urea. Moreover, Bio-Mos group revealed significantly increased serum Hemoglobin, PCV, RBCs, MCV and RBCs/ lymphocyte ratio, and significantly decreased serum SOD and CAT activities. Additionally, internal organs (liver, kidney and intestine) of groups received feed additives revealed less deteriorative change in histopathological investigations in comparison with the control group. In conclusion, supplementation of contaminated feed with ochratoxin-A by feed-additives improves feed intake and final body weight and reduces mortality rate of rabbits. In addition, it improves liver and kidney functions and reduces its pathological changes, and restores antioxidant to its normal level. Key words : rabbits; ochratoxin-A; growth performance; anti-oxidant

Offering the food to Japanese quails may help to reorganize their behavioral needs. However, limited data was reported on the effect of feeder shape on birds’ behaviors, welfare, and productivity. Herein, the main objective was to assess the effects of the most available feeder shapes in the Egyptian market on the birds’ behavior s. The shape of feeders were longitudinal, vertical-narrow, vertical-wide and round. All the used feeders were made from plastic, marked with a grey color and enriched with a net. A total of 180 one-day old Japanese quail ( Coturnix japonica ) were divided into four experimental groups (three replicates of each). Behavioral patterns of birds were investigated by direct observation and video recording for four days per week using scan sampling technique. The results indicated that there were feeder shape-dependent-differences in quail behaviors demonstrating the highest significant ( P

Studying the behavioural patterns of animals in their house may help to understand their needs, but there has been a little investigation of home-cage behaviours in commonly used inbred strains of mice such as C57BL/6 and DBA/2. Therefore, understanding behavioural patterns in these mice is important for neuroscience research. For the first time, this experiment was carried out to investigate the long-term effects of housing conditions (single vs. group) on home cage behaviour of C57BL/6 and DBA/2 mice, in order to reveal differences between C57BL/6 and DBA/2 in home-cage behaviours and in response to single-housing. Sixty-four mice (32 mice/strain) were housed either singly (SH) (n= 32) or in four groups, each group contained 8 animals. Home cage behavioural patterns were recorded weekly using ethogram-based instantaneous sampling for 5 consecutive weeks. Regardless of strain, single housed (SH) mice displayed higher levels of grooming and bedding directed-behaviours and were more frequently seen in-the-crawl ball, and had lower levels of feeding behaviour compared to their group housed (GH) conspecifics. There were significant strain differences in anxiety-related behaviours with the DBA/2 strain demonstrating higher levels of sleep, feeding and grooming behaviour and frequent presence in-the-crawl ball, and lower levels of exploration, locomotion and bedding-directed behaviour compared to the C57BL/6 strain. The results therefore suggest that different housing systems influence home cage behaviours of laboratory mice with the mice of the DBA/2 strain appearing more anxious. These findings may also have great implications for researchers to decide the most appropriate phenotype to use in measuring neural response–relevant behaviours in novel animal/human models. Key words: anxiety; behaviour; C57BL/6; DBA/2; ethogram; home-cage

Multiple factors can affect the risk of bruising and mortality of broilers during loading, transport, and lairage. The risk factors affecting the percentages of broilers in each load that were “dead-on-arrival” (DOA) or bruised were studied from records provided by a processing plant, by undertaking direct observations during on-farm loading and then carrying out multivariable analyses. Selected loads between 2014 and 2015 from seven producers were included in the study. The median DOA per load was 0.13% (Q1 = 0.06, Q3 = 0.25, n = 212), the median total duration from loading to unloading was 8.6 h, and the external temperature ranged from −22 to 22 °C. Although it was not possible to adequately characterise thermal conditions within each load, the analysis indicated that the main risk factors for increased mortality were in spring and winter, an increased duration between loading and end of lairage, and a period of feed withdrawal before loading longer than 6 h. The risk of mortality increased with the weight of the birds and with an increase in rearing mortality. No relationships were found between the manner in which the broilers were handled and the percentages of DOAs or bruised birds.

This study aimed to evaluate the effects of different monochromatic LED light colors (blue “BL”, red “RL”, green “GL” and white “WL”) in addition to natural day light (NL) on the growth performance, behavior and immune-physiological responses of goldfish (Carassius auratus). Fish (N = 75, 4.483 ± 0.061 g) were arbitrarily allocated to five groups (BL, RL, GL, WL and NL) in triplicates and fed a commercial diet (400 g/kg crude protein, 80 g/kg crude lipid) for 2 months. There was a significant improvement in final body weight, weight gain, weight gain rate, feed conversion ratio, specific growth rate, length, hepato-somatic and viscerosomatic index of fish reared under BL in respect to the other tested groups (p ≤ 0.05). Fish reared in BL showed better behavior response in form of lower ventilation frequency, longer reaction time and swimming duration in the novel object test, as well as shorter latency to first movement in duration of appetite inhibition test compared to the other experimental groups (p ≤ 0.05). Immune-physiological responses were improved in fish reared under BL followed by GL in respect to the other groups (p ≤ 0.05). This was manifested by higher red blood cells, hemoglobin, total protein, albumin (A), globulin (G), A/G ratio, lysozyme activity, as well as lower neutrophils / lymphocytes ratio, glucose, and cortisol. Despite of absence of statistical significance effect of light color on the relative expression of transforming growth factor beta 1, tumor necrosis factor alpha and interleukin-1 beta 2 genes, slight up-regulation of immune-related genes was observed in the fish reared in BL. In conclusion, rearing of goldfish under monochromatic BL improves their performance, behavior, and immune-physiological responses to stress without any adverse effects on water quality.

The present study evaluated the possible synergistic impacts of sodium butyrate and Spirulina platensis as a feed additive on growth performance, carcass composition, blood health, and intestinal histomorphology of Nile tilapia (Oreochromis niloticus). A control and three treated diets were supplemented with sodium butyrate (SB) 350 mg, Spirulina platensis (SP) 1g and a mixture of 350 mg SB and 1g SP/kg diet (SB/SP) respectively, were fed for monosex Nile tilapia (12.5± 0.50 g, n=120) for two months. The results revealed higher growth performance (final weight, weight gain, weight gain rate, SGR and PER) compared to control group (CG) (P

The aim of this study was to evaluate the impact of reduced water column in different periods on health and welfare of the Nile tilapia during harvesting. As a common practice for gathering and harvesting of tilapia in Egypt, the water column was reduced gradually until reach the lowest level (30 cm). The water column was reduced from 150 to 30 cm in different periods as follow; Group1 (G1, control group), at which the water column was reduced from 150 to 30 cm in 72 hour (h). Group 2 (G2), at which the water column was reduced from 150 to 30 cm in 48 h. Group 3(G3), at which the water column was reduced from 150 to 30 cm in 24 h. The results showed that, there was a significant (P˂0.05) decrease in muscle total bacterial count (TBC), fish injuries, heterophil /lymphocyte (L/H) ratio, serumglucose, Lactate dehydrogenase, alkaline phosphatase and an increase in water, skin TBC, dissolved oxygen (DO), unionized ammonia (UIA), and total leucocyte count (TLC) in G3 compared to G2 and G1.In conclusion, the process of reduced water column before gathering and harvesting needs to be carried out within 24 h to reduce injuries and bacterial load of fish. In addition, it improves the immune system and health of fish that reducing therisk of infection. Therefore, slow reduction of water column height during harvesting produces crowding stress altering health status and welfare of Nile tilapia (Oreochromis niloticus).

Probiotics and herbals are highly recommended in aquaculture; however, little is known about their combined effects. Therefore, the present study evaluated the possible synergistic impacts of Spirulina platensis (SP) and Bacillus amyloliquefaciens (BA) on the growth performance, histomorphology, and immune response of Nile tilapia (Oreochromis niloticus). A control and three test diets were enriched with SP, BA, or both SP and BA and fed for tilapia for 60 days. The obtained results displayed higher final body weight, weight gain, and specific growth rate in fish fed SP or/and BA with respect to the control with the highest being in fish fed both SP and BA (P

Burullus lagoon is the second largest lake in Egypt. However, there has never been a comprehensive survey which studied nineteen potentially toxic elements in sediments and plants and evaluated the associated potential risk. Thus, we aimed to study the total and potentially available content of As, Al, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Sb, Se, Sn, Tl, V, and Zn in the sediments and common reed (Phragmites australis) at thirty two sites along the entire lagoon and connected drains. Contamination Factor (CF), Pollution Load Index (PLI), Geo-accumulation Index (Igeo), and Enrichment Factor (EF) were calculated to assess the grade of contamination. Element accumulation factor (AF) and bio-concentration ratio (BCR) were also calculated. Aluminum showed the highest median (mg kg−1) total content (41,200), followed by Fe (30,300), Mn (704.7), V (82.0), Zn (75.5), Cr (51.2), Cu (47.8), Ni (44.3), As (31.9), Tl (24.6), Co (21.4), Se (20.3), Sb (17.6), Sn (15.6), Mo (11.3), and Hg (16.6 μg kg−1). Values of the EF, CF, and Igeo showed that the sediments were heavily contaminated with As, Sb, Se, Tl, Mo, Sn, Co, Ni, and Cu. The drained sediment had significantly higher values of total and potentially available element content than the lagoon sediments. Sediments of the middle and western area showed significantly higher contents of total and available elements than the eastern section. The BCR and AF values indicate that the studied plant is efficient in taking up high amounts of Zn, Fe, As, Sn, Tl, Ni, Mo, Mn; then Co, Cu, and V. The results exhibit a dramatic contamination at certain sites of the lagoon, and the studied PTEs have a predominant role in contamination-related ecological risk. Further investigations concerning redox-induced mobilization of PTEs in sediments, the risk of fish contamination and the potential health hazards are highly recommended.