1. 6-Dimethyl amino purine and 2-amino purine inhibit the induction of expresion of milk protein genes by prolactin
- Author
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Rachel Malienou-Ngassa, Mahasti Bayat-Sarmadi, Louis-Marie Houdebine, Claudine Puissant, ProdInra, Migration, Unité de biologie cellulaire et moléculaire, and Institut National de la Recherche Agronomique (INRA)
- Subjects
Purine ,[SDV]Life Sciences [q-bio] ,Biophysics ,Gene Expression ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Mammary Glands, Animal ,0302 clinical medicine ,Animals ,RNA, Messenger ,2-Aminopurine ,Protein kinase A ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,Cells, Cultured ,Protein kinase C ,030304 developmental biology ,0303 health sciences ,Messenger RNA ,biology ,Kinase ,Adenine ,Caseins ,Cell Biology ,Milk Proteins ,Molecular biology ,Prolactin ,3. Good health ,[SDV] Life Sciences [q-bio] ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Female ,Rabbits ,Whey Acidic Protein ,Signal transduction - Abstract
Two protein kinase-inhibitors, 6-dimethyl amino purine and 2-amino purine inhibited induction of β-casein synthesis by prolactin when added to the culture medium of rabbit mammary explant and cells. The accumulation of the mRNA for αs1- and β-caseins and for whey acidic protein did not take place in the presence of the inhibitors whereas β-actin mRNA concentration was not altered. In the same experimental conditions, H7, an inhibitor of protein kinase C and, to a lower extent, of protein kinase A did not prevent prolactin from acting. These data suggest for the first time that specific protein kinases are involved in the transduction of the prolactin signal to milk protein genes.
- Published
- 1992