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Hormone responsive elements within the upstream sequences of the rabbit whey acidic protein (WAP) gene direct chloramphenicol acetyl transferase (CAT) reporter gene expression in transfected rabbit mammary cells
- Source :
- Molecular and Cellular Endocrinology, Molecular and Cellular Endocrinology, Elsevier, 1991, 81 (1-3), pp.185-193
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- Whey acidic protein gene transcription is induced in the mammary gland under the influence of lactogenic hormones: prolactin, insulin and cortisol. The rabbit WAP gene has already been isolated and sequenced in a previous work. In the present study, we have evaluated the role of the 5' flanking region of the rabbit WAP gene in the transcriptional regulation of the WAP gene by using a reporter CAT gene. Chimeric genes containing the upstream region of the WAP gene have been linked to the bacterial CAT gene and transfected into rabbit primary mammary cells. The results reported here show that two regions carrying important regulatory elements of the rabbit WAP gene are located between -6300 and -3000 bp, and between -3000 and -1800 bp upstream from the WAP transcription start point, respectively. The contribute to the high level of expression of the rabbit WAP gene in the mammary cell.
- Subjects :
- Chloramphenicol O-Acetyltransferase
Hydrocortisone
[SDV]Life Sciences [q-bio]
Response element
Chimeric gene
Regulatory Sequences, Nucleic Acid
Transfection
Biochemistry
LOCALISATION
03 medical and health sciences
Mammary Glands, Animal
0302 clinical medicine
Endocrinology
Gene expression
Animals
Insulin
Lactation
Molecular Biology
Gene
Cells, Cultured
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Regulation of gene expression
0303 health sciences
Reporter gene
biology
Milk Proteins
beta-Galactosidase
Molecular biology
Recombinant Proteins
Prolactin
Gene Expression Regulation
CULTURE DE CELLULE
Regulatory sequence
030220 oncology & carcinogenesis
biology.protein
Female
Rabbits
Whey Acidic Protein
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Endocrinology
- Accession number :
- edsair.doi.dedup.....3dde63202778804ae0bd9e4ce410cd5d