16 results on '"R. Guru Raj Rao"'
Search Results
2. Contributors
- Author
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Muhammad Altaf, Muhammad Amin, T. Angelin Swetha, A. Arun, Raja Shahid Ashraf, Hasan Ay, Humaira Ayub, P. Balaji, S. Bandehali, Douglas Barbieri, Prashant Baredar, Aruna K. Behura, Muhammed Bekmezci, Priyanjali Bhattacharya, Daniela Alonso Bocchini, Abhispa Bora, Mayank Chhabra, Woon Chan Chong, Verónica Leticia Colin, Classius Ferreira da Silva, Roberto da Silva, Josiani de Cassia Pereira, Luciana Melisa Del Gobbo, Raísa Déli de Oliveira Sanches, G. Dharani, Gaurav Dwivedi, Carlos Eduardo de Oliveira do Nascimento, José Erick Galindo Gomes, Gitashree Gogoi, Eleni Gomes, Eksha Guliani, R. Guru Raj Rao, S.M. Hosseini, Christine Jeyaseelan, Ayesha Khalid, Taous Khan, Anwesha Khanra, Mohanrasu Kulanthaisamy, Soon Onn Lai, Steven Lim, Ahmed Madni, Tarun K. Maji, Shadpour Mallakpour, Moon Mandal, Sayak Mitra, A.R. Moghadassi, Gulzar Muhammad, Mina Naghdi, S. Nalini, Deise Ochi, Gabriela Okamura da Silva, Yean Ling Pang, Logeshwaran Panneerselvan, F. Parvizian, Trupti N. Patel, Cristiana Maria Pedroso Yoshida, Monika Prakash Rai, Rajiv Prakash, Muhammad Arshad Raza, Andresa Ferreira Reis, Macarena María Rulli, B.S. Saini, Sumaira Saleem, S. Sathiyamurthi, Fatih Sen, Shampa Sen, Patrícia Severino, Munazza Shahid, Waldir Eduardo Simioni Pereira, N.B. Singh, G. Sivaprakash, Eliana B. Souto, David Spressão de Lima Junior, M.P. Sudhakar, Shrasti Vasistha, S. Venkatnarayanan, Anna Cecília Venturini, and Fazli Wahid
- Published
- 2023
3. Bioremediation Process by Marine Microorganisms
- Author
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A. Arun, R. Guru Raj Rao, Rathinam Raja, and K. Mohanrasu
- Subjects
Marine pollution ,Biogeochemical cycle ,Marine bacteriophage ,Bioremediation ,Scientific method ,Microorganism ,Environmental chemistry ,Biofilm ,Environmental science - Published
- 2020
4. List of contributors
- Author
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Amitabha Acharya, Katya M. Aguilar-Pérez, Bhavna Alke, V. Ananthi, T. Angelin Swetha, A. Arun, Govindan Balasubramani, Monisha Banerjee, Hamed Barabadi, Maitree Bhattacharyya, Thulasinathan Boobalan, Abhispa Bora, Ariel P. Brown, Abdullah Çağlar, Zafer Ceylan, David Medina Cruz, G.H. Dinesh, Debjani Dutta, Ramakrishnan Geethalakshmi, Muthusamy Govarthanan, R. Guru Raj Rao, Habsah Hasan, Selcuk Hazir, Omid Hosseini, Chaudhery Mustansar Hussain, Hafiz M.N. Iqbal, J. Jeyakanthan, Kamyar Jounaki, Stefan Jurga, N.S. Kumar, P. Kumar, Nazan Kutlu, Ali Kılıçer, Lalita Ledwani, GS Lekshmi, Barbara M. Maciejewska, Mohammad Ali Mahjoub, Jennifer R. McCall, Samuel H. McCall, Dora I. Medina, Raciye Meral, Dasmawati Mohamad, K. Mohanrasu, Hamed Morad, Ebrahim Mostafavi, Sudip Nag, Anamika Nayak, J. Nirgund, SR Nivaz, Ebenezer Samuel James Obeth, Roberto Parra-Saldivar, Shama Parveen, Elahe Pishgahzadeh, Arnab Pramanik, Arivalagan Pugazhendhi, K.N. Purana, R Hari Krishna Raj, R. Karthik Raja, Shiwani Randhawa, Gustavo Ruiz-Pulido, Salar Sadeghian-Abadi, Himanshi Saini, Muthupandian Saravanan, Kathryn T. Sausman, D. Selvakumar, Chandni Sharma, S. Sil, N.B. Singh, Abdul Razack Sirajunnisa, G. Sivaprakash, Duraiarasan Surendhiran, Usman Taqui Syed, Syed Noeman Taqui, Oktay Tomar, Linh B. Truong, Shareefraza J. Ukkund, Hossein Vahidi, Mohini Verma, Karolina Wieszczycka, and Marta Woźniak-Budych
- Published
- 2022
5. Microbial bio-based polymer nanocomposite for food industry applications
- Author
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K. Mohanrasu, R. Guru Raj Rao, V. Ananthi, G. Sivaprakash, G.H. Dinesh, T. Angelin Swetha, J. Jeyakanthan, and A. Arun
- Published
- 2022
6. Production and characterization of biodegradable polyhydroxybutyrate by Micrococcus luteus isolated from marine environment
- Author
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G.H. Dinesh, Muthusamy Govarthanan, Jeyaraman Jeyakanthan, Kunyu Zhang, K. Mohanrasu, Arivalagan Pugazhendhi, A. Arun, R. Guru Raj Rao, Kumar Ponnuchamy, and Muniyasamy Sudhakar
- Subjects
Geologic Sediments ,Time Factors ,Microorganism ,Hydroxybutyrates ,macromolecular substances ,Biochemistry ,Polyhydroxybutyrate ,chemistry.chemical_compound ,Industrial Microbiology ,Marine bacteriophage ,Structural Biology ,Bioreactor ,Ammonium ,Food science ,Molecular Biology ,biology ,Molecular Structure ,Chemistry ,technology, industry, and agriculture ,Temperature ,General Medicine ,Biodegradation ,Hydrogen-Ion Concentration ,biology.organism_classification ,Butyrates ,Micrococcus luteus ,Fermentation ,lipids (amino acids, peptides, and proteins) - Abstract
Marine microorganisms are reported to produce polyhydroxybutyrate (PHB) that has wide range of medical and industrial applications with the advantage of biodegradability. PHBs are synthesized as an energy and carbon storage element under metabolic pressure. The scope of this work is enhancing PHB production using marine microbial isolate, Micrococcus luteus by selectively optimizing various growth conditions such as different media components and growth parameters that influence the cell growth and PHB production were sampled. Micrococcus luteus produced 7.54 g/L of PHB utilizing glucose as a carbon source and ammonium sulphate as a nitrogen source with maximum efficiency. The same optimized operational conditions were further employed in batch fermentation over a time span of 72 h. Interestingly higher cell dry weight of 21.52 g/L with PHB yield of 12.18 g/L and 56.59% polymer content was observed in batch fermentation studies at 64 h. The chemical nature of the extracted polymer was validated with physio-chemical experiments and was at par with the commercially available PHB. This study will spotlight M. luteus as a potential source for large-scale industrial production of PHB with reducing environmental pollutions.
- Published
- 2021
7. Marine Microbial Pharmacognosy: Prospects and Perspectives
- Author
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A. Arun, K. Mohanrasu, Jeyaraman Jeyakanthan, R. Guru Raj Rao, Rathinam Raja, and Muniyasamy Sudhakar
- Subjects
Marine conservation ,Ecology ,Genetic resources ,Marine habitats ,Biology - Abstract
Modern scientific advancements and research on marine microbes has revealed their significance as producers of therapeutic products useful in treating various human diseases. Microbes in marine habitat have evolved to adapt to the harsh condition that prevails in the ocean. Their struggle to compete for space and nutrients has paved way for the synthesis of different novel enzymes possessing distinctive characteristics. Thus, marine habitat hosts many remarkable microorganisms that offer unique biologically active compounds, enzymes endowed with astonishing properties, and mechanism to survive in extreme environmental conditions. The utilization of marine biotic resources grows at an extraordinary growth rate of 12% per annum and is evident from about 4900 patents filed connected with marine genetic resources and 18,000 natural compounds. This concern has boosted research all over the world to explore the untapped potential hidden in marine microbes, which has lot of biotechnological applications that includes bioactive compounds (metabolites) for therapeutics, novel enzymes, cosmetics, and nutraceuticals. This book chapter will meticulously deliberate the utilization of marine resources by biotechnological applications for therapeutics like antibiotics, chemical compounds, biopolymer, enzymes, and various microbial biomedical purposes such as drug delivery and tissue engineering from marine biota (bacteria, fungi, and algae).
- Published
- 2020
8. A crucial review on polycyclic aromatic Hydrocarbons - Environmental occurrence and strategies for microbial degradation
- Author
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G. Siva Prakash, N. Premnath, G.H. Dinesh, R. Guru Raj Rao, Kumar Ponnuchamy, A. Arun, V. Ananthi, Govarthanan Muthusamy, and K. Mohanrasu
- Subjects
Bioaugmentation ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,Population ,Chlorella ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Biostimulation ,Bioremediation ,Fusarium ,Pseudomonas ,polycyclic compounds ,Humans ,Soil Pollutants ,Environmental Chemistry ,Polycyclic Aromatic Hydrocarbons ,Microbial biodegradation ,education ,Ecosystem ,Soil Microbiology ,0105 earth and related environmental sciences ,Pollutant ,education.field_of_study ,Acinetobacter ,biology ,Chemistry ,Cupriavidus ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Biodegradation ,biology.organism_classification ,Pollution ,020801 environmental engineering ,Sphingomonadaceae ,Biodegradation, Environmental ,Environmental chemistry ,Rhodococcus - Abstract
Over the last century, contamination of polycyclic aromatic hydrocarbons (PAHs) has risen tremendously due to the intensified industrial activities like petrochemical, pharmaceutical, insecticides and fertilizers applications. PAHs are a group of organic pollutants with adverse effects on both humans and the environment. These PAHs are widely distributed in various ecosystems including air, soil, marine water and sediments. Degradation of PAHs generally occurs through processes like photolysis, adsorption, volatilization, chemical degradation and microbial degradation. Microbial degradation of PAHs is done by the utilization of diverse microorganisms like algae, bacteria, fungi which are readily compatible with biodegrading/bio transforming PAHs into H2O, CO2 under aerobic, or CH4 under anaerobic environment. The rate of PAHs degradation using microbes is mainly governed by various cultivation conditions like temperature, pH, nutrients availability, microbial population, chemical nature of PAHs, oxygen and degree of acclimation. Several microbial species including Selenastrum capricornutum, Ralstonia basilensis, Acinetobacter haemolyticus, Pseudomonas migulae, Sphingomonas yanoikuyae and Chlorella sorokiniana are known to degrade PAHs via biosorption and enzyme-mediated degradation. Numerous bacterial mediated PAHs degradation methods are studied globally. Among them, PAHs degradation by bacterial species like Pseudomonas fluorescence, Pseudomonas aeruginosa, Rhodococcus spp., Paenibacillus spp., Mycobacterium spp., and Haemophilus spp., by various degradation modes like biosurfactant, bioaugmentation, biostimulation and biofilms mediated are also investigated. In contrarily, PAHs degradation by fungal species such as Pleurotus ostreatus, Polyporus sulphureus, Fusarium oxysporum occurs using the activity of its ligninolytic enzymes such as lignin peroxidase, laccase, and manganese peroxidase. The present review highlighted on the PAHs degradation activity by the algal, fungal, bacterial species and also focused on their mode of degradation.
- Published
- 2021
9. Identification of potential inhibitors for AIRS from de novo purine biosynthesis pathway through molecular modeling studies – a computational approach
- Author
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Jeyakanthan Jeyaraman, Jayashree Biswal, Prabhu Dhamodharan, Surekha Kanagarajan, and R. Guru Raj Rao
- Subjects
Models, Molecular ,0301 basic medicine ,Purine ,030103 biophysics ,Ribonucleotide ,Molecular Conformation ,Plasma protein binding ,Molecular Dynamics Simulation ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Structural Biology ,Catalytic Domain ,Carbon-Nitrogen Ligases ,Nucleotide ,Amino Acid Sequence ,Enzyme Inhibitors ,Binding site ,Purine metabolism ,Molecular Biology ,Peptide sequence ,chemistry.chemical_classification ,Binding Sites ,General Medicine ,Biosynthetic Pathways ,Molecular Docking Simulation ,De novo synthesis ,030104 developmental biology ,chemistry ,Biochemistry ,Purines ,Drug Design ,Protein Binding - Abstract
In cancer, de novo pathway plays an important role in cell proliferation by supplying huge demand of purine nucleotides. Aminoimidazole ribonucleotide synthetase (AIRS) catalyzes the fifth step of de novo purine biosynthesis facilitating in the conversion of Formylglycinamidine Ribonucleotide (FGAM) to Aminoimidazole Ribonucleotide (AIR). Hence, inhibiting AIRS is crucial due to its involvement in the regulation of uncontrollable cancer cell proliferation. In this study, the three-dimensional structure of AIRS from P. horikoshii OT3 was constructed based on the crystal structure from E.coli and the modeled protein is verified for stability using molecular dynamics for a time frame of 100 ns. Virtual screening and Induced Fit Docking was performed to identify the best antagonists based on their binding mode and affinity. Through mutational studies, the residues necessary for catalytic activity of AIRS were identified and among which the following residues Lys35, Asp103, Glu137 and Thr138 are important in determination of AIRS function. The mutational studies help to understand the structural and energetic characteristics of the specified residues. In addition to, Molecular Dynamics, ADME properties, Binding free-energy and Density Function Theory (DFT) calculations of the compounds were carried out to find the best lead molecule. Based on these analyses, the compound from the NCI database, NCI_121957 was adjudged as the best molecule and could be suggested as the suitable inhibitor of AIRS. In future studies, experimental validation of these ligands as AIRS inhibitors will be carried out.
- Published
- 2016
10. Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium
- Author
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R. Guru Raj Rao, K. Mohanrasu, Dong-Po Song, G.H. Dinesh, Kunyu Zhang, Jeyaraman Jeyakanthan, G. Siva Prakash, Sudhakar Muniyasamy, Arivalagan Pugazhendhi, and A. Arun
- Subjects
Chromatography ,biology ,020209 energy ,General Chemical Engineering ,Organic Chemistry ,technology, industry, and agriculture ,Energy Engineering and Power Technology ,Potassium nitrate ,macromolecular substances ,02 engineering and technology ,biology.organism_classification ,Polyhydroxyalkanoates ,Lactic acid ,Polyhydroxybutyrate ,chemistry.chemical_compound ,Fuel Technology ,020401 chemical engineering ,chemistry ,0202 electrical engineering, electronic engineering, information engineering ,Urea ,lipids (amino acids, peptides, and proteins) ,Ammonium ,Ammonium chloride ,0204 chemical engineering ,Bacillus megaterium - Abstract
Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, 1H NMR, DSC and TGA analysis, respectively.
- Published
- 2020
11. Identification of potential inhibitors for AIRS from de novo purine biosynthesis pathway through molecular modeling studies – a computational approach
- Author
-
R. Guru Raj Rao, Jayashree Biswal, Prabhu Dhamodharan, Surekha Kanagarajan, Jeyakanthan Jeyaraman, R. Guru Raj Rao, Jayashree Biswal, Prabhu Dhamodharan, Surekha Kanagarajan, and Jeyakanthan Jeyaraman
- Abstract
In cancer, de novo pathway plays an important role in cell proliferation by supplying huge demand of purine nucleotides. Aminoimidazole ribonucleotide synthetase (AIRS) catalyzes the fifth step of de novo purine biosynthesis facilitating in the conversion of formylglycinamidine ribonucleotide to aminoimidazole ribonucleotide. Hence, inhibiting AIRS is crucial due to its involvement in the regulation of uncontrollable cancer cell proliferation. In this study, the three-dimensional structure of AIRS from P. horikoshii OT3 was constructed based on the crystal structure from E. coli and the modeled protein is verified for stability using molecular dynamics for a time frame of 100 ns. Virtual screening and induced fit docking were performed to identify the best antagonists based on their binding mode and affinity. Through mutational studies, the residues necessary for catalytic activity of AIRS were identified and among which the following residues Lys35, Asp103, Glu137, and Thr138 are important in determination of AIRS function. The mutational studies help to understand the structural and energetic characteristics of the specified residues. In addition to Molecular Dynamics, ADME properties, binding free-energy, and density functional theory calculations of the compounds were carried out to find the best lead molecule. Based on these analyses, the compound from the NCI database, NCI_121957 was adjudged as the best molecule and could be suggested as the suitable inhibitor of AIRS. In future studies, experimental validation of these ligands as AIRS inhibitors will be carried out.
- Published
- 2016
- Full Text
- View/download PDF
12. Identification of Potential Inhibitors for AIRS from de novo purine biosynthesis pathway through Molecular modeling Studies - A Computational approach
- Author
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Jeyaraman Jeyakanthan, R. Guru Raj Rao, Jayashree Biswal, D. Prabhu, K. Sureka, Jeyaraman Jeyakanthan, R. Guru Raj Rao, Jayashree Biswal, D. Prabhu, and K. Sureka
- Abstract
In cancer, de novo pathway plays an important role in cell proliferation by supplying huge demand of purine nucleotides. Aminoimidazole ribonucleotide synthetase (AIRS) catalyzes the fifth step of de novo purine biosynthesis facilitating in the conversion of Formylglycinamidine Ribonucleotide (FGAM) to Aminoimidazole Ribonucleotide (AIR). Hence, inhibiting AIRS is crucial due to its involvement in the regulation of uncontrollable cancer cell proliferation. In this study, the three-dimensional structure of AIRS from P. horikoshii OT3 was constructed based on the crystal structure from E.coli and the modeled protein is verified for stability using molecular dynamics for a time frame of 100 ns. Virtual screening and Induced Fit Docking was performed to identify the best antagonists based on their binding mode and affinity. Through mutational studies, the residues necessary for catalytic activity of AIRS were identified and among which the following residues Lys35, Asp103, Glu137 and Thr138 are important in determination of AIRS function. The mutational studies help to understand the structural and energetic characteristics of the specified residues. In addition to, Molecular Dynamics, ADME properties, Binding free-energy and Density Function Theory (DFT) calculations of the compounds were carried out to find the best lead molecule. Based on these analyses, the compound from the NCI database, NCI_121957 was adjudged as the best molecule and could be suggested as the suitable inhibitor of AIRS. In future studies, experimental validation of these ligands as AIRS inhibitors will be carried out.
- Published
- 2015
- Full Text
- View/download PDF
13. A crucial review on polycyclic aromatic Hydrocarbons - Environmental occurrence and strategies for microbial degradation.
- Author
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Premnath N, Mohanrasu K, Guru Raj Rao R, Dinesh GH, Prakash GS, Ananthi V, Ponnuchamy K, Muthusamy G, and Arun A
- Subjects
- Acinetobacter, Biodegradation, Environmental, Cupriavidus, Ecosystem, Fusarium, Humans, Pseudomonas, Soil Microbiology, Sphingomonadaceae, Chlorella, Polycyclic Aromatic Hydrocarbons, Soil Pollutants
- Abstract
Over the last century, contamination of polycyclic aromatic hydrocarbons (PAHs) has risen tremendously due to the intensified industrial activities like petrochemical, pharmaceutical, insecticides and fertilizers applications. PAHs are a group of organic pollutants with adverse effects on both humans and the environment. These PAHs are widely distributed in various ecosystems including air, soil, marine water and sediments. Degradation of PAHs generally occurs through processes like photolysis, adsorption, volatilization, chemical degradation and microbial degradation. Microbial degradation of PAHs is done by the utilization of diverse microorganisms like algae, bacteria, fungi which are readily compatible with biodegrading/bio transforming PAHs into H
2 O, CO2 under aerobic, or CH4 under anaerobic environment. The rate of PAHs degradation using microbes is mainly governed by various cultivation conditions like temperature, pH, nutrients availability, microbial population, chemical nature of PAHs, oxygen and degree of acclimation. Several microbial species including Selenastrum capricornutum, Ralstonia basilensis, Acinetobacter haemolyticus, Pseudomonas migulae, Sphingomonas yanoikuyae and Chlorella sorokiniana are known to degrade PAHs via biosorption and enzyme-mediated degradation. Numerous bacterial mediated PAHs degradation methods are studied globally. Among them, PAHs degradation by bacterial species like Pseudomonas fluorescence, Pseudomonas aeruginosa, Rhodococcus spp., Paenibacillus spp., Mycobacterium spp., and Haemophilus spp., by various degradation modes like biosurfactant, bioaugmentation, biostimulation and biofilms mediated are also investigated. In contrarily, PAHs degradation by fungal species such as Pleurotus ostreatus, Polyporus sulphureus, Fusarium oxysporum occurs using the activity of its ligninolytic enzymes such as lignin peroxidase, laccase, and manganese peroxidase. The present review highlighted on the PAHs degradation activity by the algal, fungal, bacterial species and also focused on their mode of degradation., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
14. Production and characterization of biodegradable polyhydroxybutyrate by Micrococcus luteus isolated from marine environment.
- Author
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Mohanrasu K, Guru Raj Rao R, Dinesh GH, Zhang K, Sudhakar M, Pugazhendhi A, Jeyakanthan J, Ponnuchamy K, Govarthanan M, and Arun A
- Subjects
- Butyrates chemistry, Fermentation, Hydrogen-Ion Concentration, Micrococcus luteus growth & development, Molecular Structure, Temperature, Time Factors, Butyrates metabolism, Geologic Sediments microbiology, Hydroxybutyrates isolation & purification, Industrial Microbiology, Micrococcus luteus metabolism
- Abstract
Marine microorganisms are reported to produce polyhydroxybutyrate (PHB) that has wide range of medical and industrial applications with the advantage of biodegradability. PHBs are synthesized as an energy and carbon storage element under metabolic pressure. The scope of this work is enhancing PHB production using marine microbial isolate, Micrococcus luteus by selectively optimizing various growth conditions such as different media components and growth parameters that influence the cell growth and PHB production were sampled. Micrococcus luteus produced 7.54 g/L of PHB utilizing glucose as a carbon source and ammonium sulphate as a nitrogen source with maximum efficiency. The same optimized operational conditions were further employed in batch fermentation over a time span of 72 h. Interestingly higher cell dry weight of 21.52 g/L with PHB yield of 12.18 g/L and 56.59% polymer content was observed in batch fermentation studies at 64 h. The chemical nature of the extracted polymer was validated with physio-chemical experiments and was at par with the commercially available PHB. This study will spotlight M. luteus as a potential source for large-scale industrial production of PHB with reducing environmental pollutions., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
15. Effect of C/N substrates for enhanced extracellular polymeric substances (EPS) production and Poly Cyclic Aromatic Hydrocarbons (PAHs) degradation.
- Author
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Premnath N, Mohanrasu K, Guru Raj Rao R, Dinesh GH, Siva Prakash G, Pugazhendhi A, Jeyakanthan J, Govarthanan M, Kumar P, and Arun A
- Subjects
- Biodegradation, Environmental, Extracellular Polymeric Substance Matrix, Nitrogen, Hydrocarbons, Aromatic, Polycyclic Aromatic Hydrocarbons
- Abstract
Extracellular Polymeric Substances (EPS) influenced Poly Cyclic Aromatic Hydrocarbons (PAHs) degrading Klebsiella pneumoniae was isolated from the marine environment. To increase the EPS production by Klebsiella pneumoniae, several physicochemical parameters were tweaked such as different carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch, and sucrose at 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at 2 g/L), different pH, carbon/nitrogen ratio, temperature, and salt concentration were examined. Maximum EPS growth and biodegradation of Anthracene (74.31%), Acenaphthene (67.28%), Fluorene (62.48%), Naphthalene (57.84%), and mixed PAHs (55.85%) were obtained using optimized conditions such as glucose (10 g/L) as carbon source, potassium nitrate (2 g/L) as the nitrogen source at pH 8, growth temperature of 37 °C, 3% NaCl concentration and 72 h incubation period. The Klebsiella pneumoniae biofilm architecture was studied by confocal laser scanning microscopy (CLSM) and scanning electron microscope (SEM). The present study demonstrates the EPS influenced PAHs degradation of Klebsiella pneumoniae., Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
16. Identification of potential drug target in malarial disease using molecular docking analysis.
- Author
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Joseph Sahayarayan J, Soundar Rajan K, Nachiappan M, Prabhu D, Guru Raj Rao R, Jeyakanthan J, Hossam Mahmoud A, Mohammed OB, and Morgan AMA
- Abstract
Malaria caused by genus Plasmodium, is a parasite which is the main health issue for humans and about half of the population were suffered. An every year, approximately 1.2-2.7 million people died due to malaria globally. Therefore to prevent the spreading of malaria from the glob novel active drugs with specific activities are necessary. The present study aimed to identify novel drug molecule together with the bioinformatic tools for the development of active malarial drugs. As the search for latest anti malarial compound was developed, this work determined six active blends from various drug databases which possess drug-like characteristics and presents a significant anti malarial actions in in-silico level. Compound ID 300238, 889, 76569, 87324, 45678, and Z185397112are a few of the ligands were got from the Toss lab, Maybridge, Cambridge, Life chem, Bitter, and Examine drug databases and docked against hexokinase 1 protein (PDB: 1CZA) with high throughput practical screening (HTVS) using Glide v6.6. Amid the 6 compounds, compound no: 300238 from Toss lab has the greatest docking score of -9.889 kcal/mol targeting 1CZA protein. The active sites of Hexokinase I of protein were determine by using superimposition of the destination and template structure showed similar structural folds and active sites which were decidedly conserved. The quality of hexokinase I protein was considered to be sterically stable where the protein was prepared by utilizing the software protein preparation execute in the Schrodinger suite. Prepared proteins were evaluated using SAVES and the studies of molecular dynamics of the hexokinase, and the GROMACS were performed for protein-ligand complex. The low HOMO-LUMO energy gaps of the compound verified the greater stability of the molecule. Here, the tested drug candidates have good absorption, distribution, metabolism, and excretion (ADME) properties which were established by using QikProp, version 3.4 of Schrodinger., Competing Interests: None declared., (© 2020 The Authors.)
- Published
- 2020
- Full Text
- View/download PDF
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