1. Enzymatically cross-linked arabinoxylan microspheres as oral insulin delivery system
- Author
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Norberto Sotelo-Cruz, Yolanda L. López-Franco, R. Canett-Romero, Valérie Micard, Elizabeth Carvajal-Millan, Ana L. Martínez-López, Agustín Rascón-Chu, Jaime Lizardi-Mendoza, Centro de Investigación en Alimentación y Desarrollo (CIAD), Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT), Universidad de Sonora (USON), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Fondo Institucional CONACyT - Investigación en Fronteras de la Ciencia, Mexico (Grant FON.INST./31/2016), CIAD, Centro de Investigación en Alimentación y Desarrollo A.C, and Consejo Nacional de Ciencia y Tecnologia
- Subjects
Blood Glucose ,Male ,medicine.medical_treatment ,Administration, Oral ,02 engineering and technology ,Biochemistry ,arabinoxylane ,Ferulic acid ,chemistry.chemical_compound ,Drug Delivery Systems ,Structural Biology ,Spectroscopy, Fourier Transform Infrared ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Arabinoxylan ,Tissue Distribution ,0303 health sciences ,Diabetes ,Hydrogels ,General Medicine ,021001 nanoscience & nanotechnology ,Microspheres ,Cross-Linking Reagents ,Self-healing hydrogels ,Xylans ,Rheology ,0210 nano-technology ,insulin ,microsphère ,03 medical and health sciences ,Cross-linked arabinoxylans ,In vivo ,medicine ,Animals ,Humans ,Rats, Wistar ,Molecular Biology ,insuline ,030304 developmental biology ,Chromatography ,Insulin stability ,Insulin ,Laccase ,Phenolic acid ,In vitro ,arabinoxylan ,Bioavailability ,Drug Liberation ,chemistry ,Oral drug delivery ,microbeads - Abstract
Arabinoxylans (AX) microspheres with different insulin/AX mass ratio were prepared by formation of phenoxy radical issued from the ferulic acid by enzymatic oxidation (entrapped in situ of insulin). Phenolic acid content and FT-IR spectrum of unloaded and insulin-loaded AX microspheres revealed that the phenoxy radical issued from the ferulic acid by enzymatic oxidation did not interact covalently with insulin. The microspheres showed a spherical shape, smooth surface and an average diameter of particles of 320 μm. In vitro control release found that AX microspheres minimized the insulin loss in the upper GI tract, retaining high percentage (~75%) of insulin in its matrix. The stability of the secondary structure of insulin was studied by dichroism circular (CD). The CD spectra of insulin released from AX microspheres did not change according to the insulin/AX mass ratio of the microsphere. Significant hypoglycemic effects with improved insulin-relative bioavailability tested on an in vivo murine model revealed the efficacy of these enzymatically cross-linked arabinoxylans microspheres as a new oral insulin carrier.
- Published
- 2019