579 results on '"R A, North"'
Search Results
2. Characterization of physical properties of a coastal upwelling filament with evidence of enhanced submesoscale activity and transition from balanced to unbalanced motions in the Benguela upwelling region
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R. P. North, J. Dräger-Dietel, and A. Griesel
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Geography. Anthropology. Recreation ,Environmental sciences ,GE1-350 - Abstract
We combine high-resolution in situ data (acoustic Doppler current profiler (ADCP), Scanfish, and surface drifters) and remote sensing to investigate the physical characteristics of a major filament observed in the Benguela upwelling region. The 30–50 km wide and about 400 km long filament persisted for at least 40 d. Mixed-layer depths were less than 40 m in the filament and over 60 m outside of it. Observations of the Rossby number Ro from the various platforms provide the spatial distribution of Ro for different resolutions. Remote sensing focuses on geostrophic motions of the region related to the mesoscale eddies that drive the filament formation and thereby reveals |Ro|<0.1. Ship-based measurements in the surface mixed layer reveal 0.5<|Ro|<1, indicating the presence of unbalanced, ageostrophic motions. Time series of Ro from triplets of surface drifters trapped within the filament confirm these relatively large Ro values and show a high variability along the filament. A scale-dependent analysis of Ro, which relies on the second-order velocity structure function, was applied to the latter drifter group and to another drifter group released in the upwelling zone. The two releases explored the area nearly distinctly and simultaneously and reveal that at small scales ( km) Ro values are twice as large in the filament in comparison to its environment with Ro>1 for scales smaller than ∼500 m. This suggests that filaments are hotspots of ageostrophic dynamics, pointing to the presence of a forward energy cascade. The different dynamics indicated by our Ro analysis are confirmed by horizontal kinetic energy wavenumber spectra, which exhibit a power law k−α with α∼5/3 for wavelengths 2π/k smaller than a transition scale of 15 km, supporting significant submesoscale energy at scales smaller than the first baroclinic Rossby radius (Ro1∼30 km). The detected transition scale is smaller than those found in regions with less mesoscale eddy energy, consistent with previous studies. We found evidence for the processes which drive the energy transfer to turbulent scales. Positive Rossby numbers 𝒪(1) associated with cyclonic motion inhibit the occurrence of positive Ertel potential vorticity (EPV) and stabilize the water column. However, where the baroclinic component of EPV dominates, submesoscale instability analysis suggests that mostly gravitational instabilities occur and that symmetric instabilities may be important at the filament edges.
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- 2024
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3. Quantifying biomarkers of axonal degeneration in early glaucoma to find the disc at risk
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R. L. Bartlett, B. E. Frost, K. E. Mortlock, J. R. Fergusson, N. White, J. E. Morgan, R. V. North, and J. Albon
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Medicine ,Science - Abstract
Abstract To evaluate regional axonal-related parameters as a function of disease stage in primary open angle glaucoma (POAG) and visual field (VF) sensitivity. Spectral domain optical coherence tomography was used to acquire 20° scans of POAG (n = 117) or healthy control (n = 52) human optic nerve heads (ONHs). Region specific and mean nerve fibre layer (NFL) thicknesses, border NFL and peripapillary NFL, minimum rim width (MRW)/ area (MRA) and prelamina thickness; and volume were compared across POAG disease stages and with visual field sensitivity. Differences identified between early glaucoma (EG), preperimetric glaucoma (PG) and control (C) ONHs included thinner PG prelamina regions than in controls (p
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- 2022
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4. Simulation and Signal Detection of Photon Counting Lidar Data in Forested Area.
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Bowei Chen 0002, Yong Pang, Zengyuan Li, Peter R. J. North, Jacqueline A. B. Rosette, I. J. Bye, Hao Lu 0004, Liuxia Liu, and Zhenyu Ma
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- 2018
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5. OLCI/SLSTR SYN L2 Algorithm and Products Overview.
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Claire Henocq, Peter R. J. North, Andreas Heckel, Stéphane Ferron, Nicolas Lamquin, Steffen Dransfeld, Ludovic Bourg, Carolien Toté, and Didier Ramon
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- 2018
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6. Monitoring Forest Health with Sun-Induced Chlorophyll Fluorescence Observations and 3-D Radiative Transfer Modeling.
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Rocío Hernández-Clemente, Peter R. J. North, Alberto Hornero, and Pablo J. Zarco-Tejada
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- 2018
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7. Ground and Top of Canopy Extraction From Photon-Counting LiDAR Data Using Local Outlier Factor With Ellipse Searching Area.
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Bowei Chen 0002, Yong Pang, Zengyuan Li, Hao Lu 0004, Luxia Liu, Peter R. J. North, and Jacqueline Rosette
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- 2019
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8. Quantitative global mapping of terrestrial vegetation photosynthesis: The Fluorescence Explorer (FLEX) mission.
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José F. Moreno, Roberto Colombo, Alexander Damm, Yves Goulas, Elizabeth M. Middleton, Franco Miglietta, Gina Mohammed, Matti Mottus, Peter R. J. North, Uwe Rascher, Christiaan van der Tol, and Matthias Drusch
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- 2017
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9. Particulate emissions from large North American wildfires estimated using a new top-down method
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T. Nikonovas, P. R. J. North, and S. H. Doerr
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Particulate matter emissions from wildfires affect climate, weather and air quality. However, existing global and regional aerosol emission estimates differ by a factor of up to 4 between different methods. Using a novel approach, we estimate daily total particulate matter (TPM) emissions from large wildfires in North American boreal and temperate regions. Moderate Resolution Imaging Spectroradiometer (MODIS) fire location and aerosol optical thickness (AOT) data sets are coupled with HYSPLIT (Hybrid Single-Particle Lagrangian Integrated Trajectory) atmospheric dispersion simulations, attributing identified smoke plumes to sources. Unlike previous approaches, the method (i) combines information from both satellite and AERONET (AErosol RObotic NETwork) observations to take into account aerosol water uptake and plume specific mass extinction efficiency when converting smoke AOT to TPM, and (ii) does not depend on instantaneous emission rates observed during individual satellite overpasses, which do not sample night-time emissions. The method also allows multiple independent estimates for the same emission period from imagery taken on consecutive days. Repeated fire-emitted AOT estimates for the same emission period over 2 to 3 days of plume evolution show increases in plume optical thickness by approximately 10 % for boreal events and by 40 % for temperate emissions. Inferred median water volume fractions for aged boreal and temperate smoke observations are 0.15 and 0.47 respectively, indicating that the increased AOT is partly explained by aerosol water uptake. TPM emission estimates for boreal events, which predominantly burn during daytime, agree closely with bottom-up Global Fire Emission Database (GFEDv4) and Global Fire Assimilation System (GFASv1.0) inventories, but are lower by approximately 30 % compared to Quick Fire Emission Dataset (QFEDv2) PM2. 5, and are higher by approximately a factor of 2 compared to Fire Energetics and Emissions Research (FEERv1) TPM estimates. The discrepancies are larger for temperate fires, which are characterized by lower median fire radiative power values and more significant night-time combustion. The TPM estimates for this study for the biome are lower than QFED PM2. 5 by 35 %, and are larger by factors of 2.4, 3.2 and 4 compared with FEER, GFED and GFAS inventories respectively. A large underestimation of TPM emission by bottom-up GFED and GFAS indicates low biases in emission factors or consumed biomass estimates for temperate fires.
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- 2017
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10. Geoffrey Burnstock 1929–2020
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R. Alan North and Marcello Costa
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Arts and Humanities (miscellaneous) ,History and Philosophy of Science ,Human Factors and Ergonomics ,Social Sciences (miscellaneous) ,Demography - Abstract
Geoffrey Burnstock was a biomedical scientist who gained renown for his discovery that adenosine 5′-triphosphate (ATP) functions as an extracellular signalling molecule. Born in London and educated at King’s and University Colleges, he did postdoctoral work at Mill Hill and Oxford. He moved in 1959 to the Department of Zoology at the University of Melbourne because he sensed there a greater freedom to challenge established thinking in physiology. His group found that transmission from sympathetic and parasympathetic autonomic nerves to smooth muscle was in some places not mediated by the accepted chemical messengers (noradrenaline and acetylcholine). He amassed evidence that ATP was this non-adrenergic, non-cholinergic (NANC) transmitter, using biochemical, histological and electrophysiological approaches: heretically, he styled this ‘purinergic transmission’. Geoff further upset dogma in the 1970s by proposing ‘co-transmission’ in which some nerves released ATP in addition to either noradrenaline or acetylcholine. He distinguished pharmacologically P1 receptors (activated best by adenosine and blocked by xanthines) and P2 receptors (activated best by purine nucleotides such as ATP) and he proposed in 1985 that the latter embraced P2X (ion channel) and P2Y (G protein-coupled) subtypes: about ten years later these categories were substantiated by cDNA cloning. From 1975 until his retirement in 1997, Geoff was head of Anatomy and Embryology at University College London (UCL), which he developed energetically into a large and strong research department. Later, as head of the Autonomic Research Institute at the Royal Free (part of UCL), he continued to collaborate extensively, and founded several journals and international professional societies. He widely sought clinical benefit for his discoveries, and both P2X and P2Y receptors have been developed as the targets of useful therapeutics (gefapixant, clopidogrel). Geoff was proud of his modest, rather humble, background and eschewed formality. He may have smiled when his early discoveries were met with cynicism, even ridicule (‘pure-imagine’ transmission noted one amusing critic), but this just reinforced his resolve and encouraged his encyclopaedic oeuvre.
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- 2022
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11. The ESA globAlbedo project: Algorithm.
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Philip Lewis, Luis Guanter, Gerardo López Saldaña, Jan-Peter Muller, Gill Watson, Neville Shane, Tom Kennedy, Jürgen Fischer, Carlos Domenech, Rene Preusker, Peter R. J. North, Andreas Heckel, Olaf Danne, Uwe Krämer, Marco Zühlke, Norman Fomferra, Carsten Brockmann, and Crystal Schaaf
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- 2012
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12. Plagiarism Reimagined
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R A North
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General Medicine - Published
- 2023
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13. Smoke aerosol properties and ageing effects for northern temperate and boreal regions derived from AERONET source and age attribution
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T. Nikonovas, P. R. J. North, and S. H. Doerr
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Particulate emissions from wildfires impact human health and have a large but uncertain effect on climate. Modelling schemes depend on information about emission factors, emitted particle microphysical and optical properties and ageing effects, while satellite retrieval algorithms make use of characteristic aerosol models to improve retrieval. Ground-based remote sensing provides detailed aerosol characterisation, but does not contain information on source. Here, a method is presented to estimate plume origin land cover type and age for AERONET aerosol observations, employing trajectory modelling using the HYSPLIT model, and satellite active fire and aerosol optical thickness (AOT) observations from Moderate Resolution Imaging Spectroradiometer (MODIS) and Along Track Scanning Radiometer (AATSR). It is applied to AERONET stations located in or near northern temperate and boreal forests for the period 2002–2013. The results from 629 fire attributions indicate significant differences in size distributions and particle optical properties between different land cover types and plume age. Smallest fine mode median radius (Rfv) are attributed to plumes from cropland and/or natural vegetation mosaic (0.143 μm) and grassland (0.157 μm) fires. North American evergreen needleleaf forest emissions show a significantly smaller Rfv (0.164 μm) than plumes from Eurasian mixed forests (0.193 μm) and plumes attributed to the land cover types with sparse tree cover – open shrubland (0.185 μm) and woody savannas (0.184 μm). The differences in size distributions are related to inferred variability in plume concentrations between the land cover types. Significant differences are observed between day and night emissions, with daytime emissions showing larger particle sizes. Smoke is predominantly scattering for all of the classes with median single scattering albedo at 440 nm (SSA(440)) values close to 0.95 except the cropland emissions which have an SSA(440) value of 0.9. Plumes aged for 4 days or older have median Rfv larger by ~0.02 μm compared to young smoke. Differences in size were consistent with a decrease in the Ångström Exponent and increase in the asymmetry parameter. Only an insignificant increase in SSA(λ) with ageing was found.
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- 2015
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14. Synergistic angular and spectral estimation of aerosol properties using CHRIS/PROBA-1 and simulated Sentinel-3 data
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W. H. Davies and P. R. J. North
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Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
We develop a method to derive aerosol properties over land surfaces using combined spectral and angular information, such as available from ESA Sentinel-3 mission, to be launched in 2015. A method of estimating aerosol optical depth (AOD) using only angular retrieval has previously been demonstrated on data from the ENVISAT and PROBA-1 satellite instruments, and is extended here to the synergistic spectral and angular sampling of Sentinel-3. The method aims to improve the estimation of AOD, and to explore the estimation of fine mode fraction (FMF) and single scattering albedo (SSA) over land surfaces by inversion of a coupled surface/atmosphere radiative transfer model. The surface model includes a general physical model of angular and spectral surface reflectance. An iterative process is used to determine the optimum value of the aerosol properties providing the best fit of the corrected reflectance values to the physical model. The method is tested using hyperspectral, multi-angle Compact High Resolution Imaging Spectrometer (CHRIS) images. The values obtained from these CHRIS observations are validated using ground-based sun photometer measurements. Results from 22 image sets using the synergistic retrieval and improved aerosol models show an RMSE of 0.06 in AOD, reduced to 0.03 over vegetated targets.
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- 2015
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15. Serendipity in senescence
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R A North
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- 2022
16. Global Atmospheric Aerosol Optical Depth Retrievals over Land and Ocean from AATSR.
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Suzanne Bevan, Peter R. J. North, William M. F. Grey, and Sietse O. Los
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- 2009
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17. Slope Estimation from ICESat/GLAS
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Craig Mahoney, Natascha Kljun, Sietse O. Los, Laura Chasmer, Jorg M. Hacker, Christopher Hopkinson, Peter R. J. North, Jacqueline A. B. Rosette, and Eva van Gorsel
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LiDAR ,slope ,terrain ,waveform ,SRTM ,biophysical parameter retrieval ,Science - Abstract
We present a novel technique to infer ground slope angle from waveform LiDAR, known as the independent slope method (ISM). The technique is applied to large footprint waveforms (\(\sim\) mean diameter) from the Ice, Cloud and Land Elevation Satellite (ICESat) Geoscience Laser Altimeter System (GLAS) to produce a slope dataset of near-global coverage at \(0.5^{\circ} \times 0.5^{\circ}\) resolution. ISM slope estimates are compared against high resolution airborne LiDAR slope measurements for nine sites across three continents. ISM slope estimates compare better with the aircraft data (R\(^{2}=0.87\) and RMSE\(=5.16^{\circ}\)) than the Shuttle Radar Topography Mission Digital Elevation Model (SRTM DEM) inferred slopes (R\(^{2}=0.71\) and RMSE\(=8.69^{\circ}\)) ISM slope estimates are concurrent with GLAS waveforms and can be used to correct biophysical parameters, such as tree height and biomass. They can also be fused with other DEMs, such as SRTM, to improve slope estimates.
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- 2014
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18. Response of vegetation to the 2003 European drought was mitigated by height
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S. L. Bevan, S. O. Los, and P. R. J. North
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Ecology ,QH540-549.5 ,Life ,QH501-531 ,Geology ,QE1-996.5 - Abstract
The effects on climate of land-cover change, predominantly from the conversion of forests to crops or grassland, are reasonably well understood for low and high latitudes but are largely unknown for temperate latitudes. The main reason for this gap in our knowledge is that there are compensating effects on the energy and water balance that are related to changes in land-surface albedo, soil evaporation and plant transpiration. We analyse how vegetation height affected the response of vegetation during the 2003 European drought using precipitation data, temperature data, normalized difference vegetation index data and a new vegetation height data set obtained from the Geoscience Laser Altimeter System (GLAS) on the Ice, Cloud and land Elevation Satellite (ICESat). At the height of the 2003 drought we find for tall vegetation a significantly smaller decrease in vegetation index and a smaller diurnal temperature (DTR) range, indicating less water stress and drought impacts on tall vegetation. Over Germany for example, 98% of significant correlations showed a smaller anomaly in vegetation index anomaly with greater height, and 95% of significant correlations showed a smaller DTR with greater vegetation height. Over France the equivalent percentages were 94 and 88%, respectively. Vegetation height is likely associated with greater rooting depth, canopy heat capacity or both. Our results suggest that land-surface models can be improved by better estimates of vegetation height and associated with this a more realistic response to drought.
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- 2014
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19. Evaluating Prospects for Improved Forest Parameter Retrieval From Satellite LiDAR Using a Physically-Based Radiative Transfer Model.
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Jacqueline Rosette, Peter R. J. North, Jérémy Rubio-Gil, Bruce D. Cook, Sietse O. Los, Juan Suárez, Guoqing Sun, K. Jon Ranson, and J. Bryan Blair
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- 2013
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20. Statistical Distances and Their Applications to Biophysical Parameter Estimation: Information Measures, M-Estimates, and Minimum Contrast Methods.
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Ganna Leonenko, Sietse O. Los, and Peter R. J. North
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- 2013
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21. Investigating hypoxia in aquatic environments: diverse approaches to addressing a complex phenomenon
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J. Friedrich, F. Janssen, D. Aleynik, H. W. Bange, N. Boltacheva, M. N. Çagatay, A. W. Dale, G. Etiope, Z. Erdem, M. Geraga, A. Gilli, M. T. Gomoiu, P. O. J. Hall, D. Hansson, Y. He, M. Holtappels, M. K. Kirf, M. Kononets, S. Konovalov, A. Lichtschlag, D. M. Livingstone, G. Marinaro, S. Mazlumyan, S. Naeher, R. P. North, G. Papatheodorou, O. Pfannkuche, R. Prien, G. Rehder, C. J. Schubert, T. Soltwedel, S. Sommer, H. Stahl, E. V. Stanev, A. Teaca, A. Tengberg, C. Waldmann, B. Wehrli, and F. Wenzhöfer
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Ecology ,QH540-549.5 ,Life ,QH501-531 ,Geology ,QE1-996.5 - Abstract
In this paper we provide an overview of new knowledge on oxygen depletion (hypoxia) and related phenomena in aquatic systems resulting from the EU-FP7 project HYPOX ("In situ monitoring of oxygen depletion in hypoxic ecosystems of coastal and open seas, and landlocked water bodies", http://www.hypox.net). In view of the anticipated oxygen loss in aquatic systems due to eutrophication and climate change, HYPOX was set up to improve capacities to monitor hypoxia as well as to understand its causes and consequences. Temporal dynamics and spatial patterns of hypoxia were analyzed in field studies in various aquatic environments, including the Baltic Sea, the Black Sea, Scottish and Scandinavian fjords, Ionian Sea lagoons and embayments, and Swiss lakes. Examples of episodic and rapid (hours) occurrences of hypoxia, as well as seasonal changes in bottom-water oxygenation in stratified systems, are discussed. Geologically driven hypoxia caused by gas seepage is demonstrated. Using novel technologies, temporal and spatial patterns of water-column oxygenation, from basin-scale seasonal patterns to meter-scale sub-micromolar oxygen distributions, were resolved. Existing multidecadal monitoring data were used to demonstrate the imprint of climate change and eutrophication on long-term oxygen distributions. Organic and inorganic proxies were used to extend investigations on past oxygen conditions to centennial and even longer timescales that cannot be resolved by monitoring. The effects of hypoxia on faunal communities and biogeochemical processes were also addressed in the project. An investigation of benthic fauna is presented as an example of hypoxia-devastated benthic communities that slowly recover upon a reduction in eutrophication in a system where naturally occurring hypoxia overlaps with anthropogenic hypoxia. Biogeochemical investigations reveal that oxygen intrusions have a strong effect on the microbially mediated redox cycling of elements. Observations and modeling studies of the sediments demonstrate the effect of seasonally changing oxygen conditions on benthic mineralization pathways and fluxes. Data quality and access are crucial in hypoxia research. Technical issues are therefore also addressed, including the availability of suitable sensor technology to resolve the gradual changes in bottom-water oxygen in marine systems that can be expected as a result of climate change. Using cabled observatories as examples, we show how the benefit of continuous oxygen monitoring can be maximized by adopting proper quality control. Finally, we discuss strategies for state-of-the-art data archiving and dissemination in compliance with global standards, and how ocean observations can contribute to global earth observation attempts.
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- 2014
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22. Correction: Yahui, Che et al. Validation of Aerosol Products from AATSR and MERIS/AATSR Synergy Algorithms - Part 1: Global Evaluation. Remote Sensing 2018, 10, 1414.
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Yahui Che, Linlu Mei, Yong Xue, Jie Guang, Lu She, Ying Li 0035, Andreas Heckel, and Peter R. J. North
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- 2019
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23. Potential of Forest Parameter Estimation Using Metrics from Photon Counting LiDAR Data in Howland Research Forest.
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Bowei Chen 0002, Yong Pang, Zengyuan Li, Peter R. J. North, Jacqueline Rosette, Guoqing Sun, Juan Suárez, Iain Bye, and Hao Lu 0004
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- 2019
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24. Improvements in Aerosol Optical Depth Estimation Using Multiangle CHRIS/PROBA Images.
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William H. Davies, Peter R. J. North, William M. F. Grey, and Michael J. Barnsley
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- 2010
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25. Supplementary material to 'Extended validation and evaluation of the OLCI-SLSTR Synergy aerosol product (SY_2_AOD) on Sentinel-3'
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Larisa Sogacheva, Matthieu Denisselle, Pekka Kolmonen, Timo H. Virtanen, Peter R. J. North, Claire Henocq, Silvia Scifoni, and Steffen Dransfeld
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- 2022
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26. Intercomparison of desert dust optical depth from satellite measurements
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E. Carboni, G. E. Thomas, A. M. Sayer, R. Siddans, C. A. Poulsen, R. G. Grainger, C. Ahn, D. Antoine, S. Bevan, R. Braak, H. Brindley, S. DeSouza-Machado, J. L. Deuzé, D. Diner, F. Ducos, W. Grey, C. Hsu, O. V. Kalashnikova, R. Kahn, P. R. J. North, C. Salustro, A. Smith, D. Tanré, O. Torres, and B. Veihelmann
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Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
This work provides a comparison of satellite retrievals of Saharan desert dust aerosol optical depth (AOD) during a strong dust event through March 2006. In this event, a large dust plume was transported over desert, vegetated, and ocean surfaces. The aim is to identify the differences between current datasets. The satellite instruments considered are AATSR, AIRS, MERIS, MISR, MODIS, OMI, POLDER, and SEVIRI. An interesting aspect is that the different algorithms make use of different instrument characteristics to obtain retrievals over bright surfaces. These include multi-angle approaches (MISR, AATSR), polarisation measurements (POLDER), single-view approaches using solar wavelengths (OMI, MODIS), and the thermal infrared spectral region (SEVIRI, AIRS). Differences between instruments, together with the comparison of different retrieval algorithms applied to measurements from the same instrument, provide a unique insight into the performance and characteristics of the various techniques employed. As well as the intercomparison between different satellite products, the AODs have also been compared to co-located AERONET data. Despite the fact that the agreement between satellite and AERONET AODs is reasonably good for all of the datasets, there are significant differences between them when compared to each other, especially over land. These differences are partially due to differences in the algorithms, such as assumptions about aerosol model and surface properties. However, in this comparison of spatially and temporally averaged data, it is important to note that differences in sampling, related to the actual footprint of each instrument on the heterogeneous aerosol field, cloud identification and the quality control flags of each dataset can be an important issue.
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- 2012
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27. Vegetation height and cover fraction between 60° S and 60° N from ICESat GLAS data
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S. O. Los, J. A. B. Rosette, N. Kljun, P. R. J. North, L. Chasmer, J. C. Suárez, C. Hopkinson, R. A. Hill, E. van Gorsel, C. Mahoney, and J. A. J. Berni
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Geology ,QE1-996.5 - Abstract
We present new coarse resolution (0.5° × 0.5°) vegetation height and vegetation-cover fraction data sets between 60° S and 60° N for use in climate models and ecological models. The data sets are derived from 2003–2009 measurements collected by the Geoscience Laser Altimeter System (GLAS) on the Ice, Cloud and land Elevation Satellite (ICESat), the only LiDAR instrument that provides close to global coverage. Initial vegetation height is calculated from GLAS data using a development of the model of Rosette et al. (2008) with with further calibration on desert sites. Filters are developed to identify and eliminate spurious observations in the GLAS data, e.g. data that are affected by clouds, atmosphere and terrain and as such result in erroneous estimates of vegetation height or vegetation cover. Filtered GLAS vegetation height estimates are aggregated in histograms from 0 to 70 m in 0.5 m intervals for each 0.5° × 0.5°. The GLAS vegetation height product is evaluated in four ways. Firstly, the Vegetation height data and data filters are evaluated using aircraft LiDAR measurements of the same for ten sites in the Americas, Europe, and Australia. Application of filters to the GLAS vegetation height estimates increases the correlation with aircraft data from r = 0.33 to r = 0.78, decreases the root-mean-square error by a factor 3 to about 6 m (RMSE) or 4.5 m (68% error distribution) and decreases the bias from 5.7 m to −1.3 m. Secondly, the global aggregated GLAS vegetation height product is tested for sensitivity towards the choice of data quality filters; areas with frequent cloud cover and areas with steep terrain are the most sensitive to the choice of thresholds for the filters. The changes in height estimates by applying different filters are, for the main part, smaller than the overall uncertainty of 4.5–6 m established from the site measurements. Thirdly, the GLAS global vegetation height product is compared with a global vegetation height product typically used in a climate model, a recent global tree height product, and a vegetation greenness product and is shown to produce realistic estimates of vegetation height. Finally, the GLAS bare soil cover fraction is compared globally with the MODIS bare soil fraction (r = 0.65) and with bare soil cover fraction estimates derived from AVHRR NDVI data (r = 0.67); the GLAS tree-cover fraction is compared with the MODIS tree-cover fraction (r = 0.79). The evaluation indicates that filters applied to the GLAS data are conservative and eliminate a large proportion of spurious data, while only in a minority of cases at the cost of removing reliable data as well. The new GLAS vegetation height product appears more realistic than previous data sets used in climate models and ecological models and hence should significantly improve simulations that involve the land surface.
- Published
- 2012
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28. The inter-comparison of major satellite aerosol retrieval algorithms using simulated intensity and polarization characteristics of reflected light
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A. A. Kokhanovsky, J. L. Deuzé, D. J. Diner, O. Dubovik, F. Ducos, C. Emde, M. J. Garay, R. G. Grainger, A. Heckel, M. Herman, I. L. Katsev, J. Keller, R. Levy, P. R. J. North, A. S. Prikhach, V. V. Rozanov, A. M. Sayer, Y. Ota, D. Tanré, G. E. Thomas, and E. P. Zege
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Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
Remote sensing of aerosol from space is a challenging and typically underdetermined retrieval task, requiring many assumptions to be made with respect to the aerosol and surface models. Therefore, the quality of a priori information plays a central role in any retrieval process (apart from the cloud screening procedure and the forward radiative transfer model, which to be most accurate should include the treatment of light polarization and molecular-aerosol coupling). In this paper the performance of various algorithms with respect to the of spectral aerosol optical thickness determination from optical spaceborne measurements is studied. The algorithms are based on various types of measurements (spectral, angular, polarization, or some combination of these). It is confirmed that multiangular spectropolarimetric measurements provide more powerful constraints compared to spectral intensity measurements alone, particularly those acquired at a single view angle and which rely on a priori assumptions regarding the particle phase function in the retrieval process.
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- 2010
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29. Aerosol optical depth and land surface reflectance from multiangle AATSR measurements: global validation and intersensor comparisons.
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William M. F. Grey, Peter R. J. North, Sietse O. Los, and Ross M. Mitchell
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- 2006
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30. Improving the Performance of 3-D Radiative Transfer Model FLIGHT to Simulate Optical Properties of a Tree-Grass Ecosystem.
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José Ramón Melendo-Vega, María Pilar Martín, Javier Pacheco-Labrador, Rosario González-Cascón, Gerardo Moreno, Fernando Pérez, Mirco Migliavacca, Mariano García, Peter R. J. North, and David Riaño 0002
- Published
- 2018
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31. Optimal Surface Fusion.
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Peter R. J. North
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- 1991
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32. Quantifying biomarkers of axonal degeneration in early glaucoma to find the disc at risk
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R. L. Bartlett, B. E. Frost, K. E. Mortlock, J. R. Fergusson, N. White, J. E. Morgan, R. V. North, and J. Albon
- Subjects
Retinal Ganglion Cells ,Multidisciplinary ,Humans ,Glaucoma ,Biomarkers ,Glaucoma, Open-Angle ,Intraocular Pressure ,Tomography, Optical Coherence - Abstract
To evaluate regional axonal-related parameters as a function of disease stage in primary open angle glaucoma (POAG) and visual field (VF) sensitivity. Spectral domain optical coherence tomography was used to acquire 20° scans of POAG (n = 117) or healthy control (n = 52) human optic nerve heads (ONHs). Region specific and mean nerve fibre layer (NFL) thicknesses, border NFL and peripapillary NFL, minimum rim width (MRW)/ area (MRA) and prelamina thickness; and volume were compared across POAG disease stages and with visual field sensitivity. Differences identified between early glaucoma (EG), preperimetric glaucoma (PG) and control (C) ONHs included thinner PG prelamina regions than in controls (p p p = 0.049) compared to control eyes; and EG mean, and inferior and ST, border NFL was thinner than in PG (p p p = 0.023). MRW differences included: PG SN and inferior less than in controls (p p p = 0.127) and prelamina volume (p
- Published
- 2021
33. Reconstruction of visual appearance.
- Author
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Peter R. J. North
- Published
- 1990
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34. Retrieval of land surface bidirectional reflectance and aerosol opacity from ATSR-2 multiangle imagery.
- Author
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Peter R. J. North, Stephen A. Briggs, Stephen E. Plummer, and Jeffery J. Settle
- Published
- 1999
- Full Text
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35. Three-dimensional forest light interaction model using a Monte Carlo method.
- Author
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Peter R. J. North
- Published
- 1996
- Full Text
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36. Development, Production and Evaluation of Aerosol Climate Data Records from European Satellite Observations (Aerosol_cci).
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Thomas Popp, Gerrit de Leeuw, Christine Bingen, Christoph Brühl, Virginie Capelle, Alain Chédin, Lieven Clarisse, Oleg Dubovik, Roy G. Grainger, Jan Griesfeller, Andreas Heckel, Stefan Kinne, Lars Klüser, Miriam Kosmale, Pekka Kolmonen, Luca Lelli, Pavel Litvinov, Linlu Mei, Peter R. J. North, Simon Pinnock, Adam C. Povey, Charles Robert, Michael Schulz, Larisa Sogacheva, Kerstin Stebel, Deborah Stein Zweers, Gareth Thomas, Lieuwe Gijsbert Tilstra, Sophie Vandenbussche, J. Pepijn Veefkind, Marco Vountas, and Yong Xue
- Published
- 2016
- Full Text
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37. Transmitter Regulation of Mesencephalic Dopamine Cells
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Vincent Seutin, R. Alan North, and Steven W. Johnson
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Dopamine ,Chemistry ,Transmitter ,medicine ,Neuroscience ,medicine.drug - Published
- 2019
- Full Text
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38. Canadian plans for participation in GSETT 3
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R. N. North
- Subjects
Canada ,seismograph network ,broad band ,array ,Meteorology. Climatology ,QC851-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The Geological Survey of Canada (GSC) is making preparations for Canadian participation in GSETT 3 but will be unable to make a formal commitment until the necessary resources have been secured. As Canada is expected to provide at least four alpha stations, and a significant number of beta stations, the financial resources that will be needed are substantial, even though in many respccts the GSC is, with the recent modernization of the Yellowknife array and the ongoing installation of the Canadian National Seismograph Network (CNSN), well positioned to make a significant contribution to GSETT 3. The CNSN currently (October 1993) consists of 17 broad band stations and will grow to 23 and 33 such stations by December 1993 and December 1994 respectively. Some 40 50 short period stations will complete the network. Data from all sites are continuously telemetered in real time to network acquisition centres in Ottawa and Sidney, British Columbia, archived to optical disk, and kept on line in a 72 h ring buffer. Most of the broadband sites could serve as either alpha or beta stations once the necessary software for continuous data transfer, or on request provision, of data from the selected sites has been completed. This software wili be configured so that changes in station selection are easy to implement, and this will provide considerable flexibility to the GSETT 3 planning and operations working groups in selecting the optimum network. Backup stations can be designated in the case of station failures, and the network centre in British Columbia will serve, at least for beta stations, as a backup NDC to that in Ottawa. Data from. the Yellowknife array are collected in Yellowknife and forwarded in ten minute files to Ottawa, where processing is completed and the results archived. This arrangement would not meet the deadlines for receipt of alpha station data at the IDC and new hardware and software will be needed to forward the data more immediately from Yellowknife to Ottawa. Although the procedures and formats for both alpha and beta station data have not yet been agreed upon, or even discussed, by the GSE, it is apparent that new facilities will be re quired in Ottawa to multiplex and reformat data for transmission to the IDC. We anticipate that a dedicated 56 kbaud link will be needed between Ottawa. and Washington.
- Published
- 1994
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39. The Canadian National Seismograph Network
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R. G. North
- Subjects
Meteorology. Climatology ,QC851-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The Canadian National Seismograph Network currently consists of 5 very-broadband (VBB) and 15 broadband (BB) stations across Canada, supplemented by 6 short period (SP) stations. When it is completed by the end of 1995, a further 1 VBB, 12 BB and over 40 SP stations will have been added. Data from all sites are telemetered in real time to twin network acquisition, processing and archiving centres in Eastern and Western Canada. All data are continuously archived in SEED format on optical disk and access to the most recent three days of data is provided through a mail-based AutoDRM system. Continuous data from the VBB sites are sent to the FDSN Data Management Centre approximately one month after being recorded.
- Published
- 1994
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- View/download PDF
40. Automated detection and association of surface waves
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C. R. D. Woodgold and R. G. North
- Subjects
surface wave ,detection ,association ,automated detection ,azimuth determination ,Meteorology. Climatology ,QC851-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
An algorithm for the automatic detection and association of surface waves has been developed and tested over an 18 month interval on broad band data from the Yellowknife array (YKA). The detection algorithm uses a conventional STA/LTA scheme on data that have been narrow band filtered at 20 s periods and a test is then applied to identify dispersion. An average of 9 surface waves are detected daily using this technique. Beamforming is applied to determine the arrival azimuth; at a nonarray station this could be provided by poIarization analysis. The detected surface waves are associated daily with the events located by the short period array at Yellowknife, and later with the events listed in the USGS NEIC Monthly Summaries. Association requires matching both arrival time and azimuth of the Rayleigh waves. Regional calibration of group velocity and azimuth is required. . Large variations in both group velocity and azimuth corrections were found, as an example, signals from events in Fiji Tonga arrive with apparent group velocities of 2.9 3.5 krn/s and azimuths from 5 to + 40 degrees clockwise from true (great circle) azimuth, whereas signals from Kuriles Kamchatka have velocities of 2.4 2.9 km/s and azimuths off by 35 to 0 degrees. After applying the regional corrections, surface waves are considered associated if the arrival time matches to within 0.25 km/s in apparent group velocity and the azimuth is within 30 degrees of the median expected. Over the 18 month period studied, 32% of the automatically detected surface waves were associated with events located by the Yellowknife short period array, and 34% (1591) with NEIC events; there is about 70% overlap between the two sets of events. Had the automatic detections been reported to the USGS, YKA would have ranked second (after LZH) in terms of numbers of associated surface waves for the study period of April 1991 to September 1992.
- Published
- 1994
- Full Text
- View/download PDF
41. Ectodomain Movements of an ATP-gated Ion Channel (P2X2 Receptor) Probed by Disulfide Locking
- Author
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Liam E. Browne, Vincent Compan, Olga Stelmashenko, and R. Alan North
- Subjects
Mutation, Missense ,Biochemistry ,Protein Structure, Secondary ,Ion Channels ,Dithiothreitol ,Cell membrane ,Disulfide ,chemistry.chemical_compound ,Adenosine Triphosphate ,Protein structure ,Membrane Biology ,medicine ,Animals ,Humans ,Disulfides ,Molecular Biology ,Ion channel ,Chemistry ,Bilayer ,Cell Membrane ,Gated Ion Channel ,Cell Biology ,Protein Structure, Tertiary ,Rats ,ATP ,Transmembrane domain ,Crystallography ,medicine.anatomical_structure ,Amino Acid Substitution ,Ectodomain ,Receptor Structure-function ,Purinergic Receptor ,Receptors, Purinergic P2X2 - Abstract
Background: The ATP-gated channel (P2X2) receptor ectodomain is formed by β-strands from three subunits. Results: ATP-induced channel opening was prevented by disulfide locking between pairs of substituted cysteines in different subunits. Conclusion: The turret region, the lower body wall, and the outer ends of transmembrane domains move significantly in channel opening. Significance: Large ectodomain movements couple ATP binding to channel opening in P2X receptors., The ectodomain of the P2X receptor is formed mainly from two- or three-stranded β-sheets provided symmetrically by each of the three subunits. These enclose a central cavity that is closed off furthest from the plasma membrane (the turret) and that joins with the transmembrane helices to form the ion permeation pathway. Comparison of closed and open crystal structures indicates that ATP binds in a pocket positioned between strands provided by different subunits and that this flexes the β-sheets of the lower body and enlarges the central cavity: this pulls apart the outer ends of the transmembrane helices and thereby opens an aperture, or gate, where they intersect within the membrane bilayer. In the present work, we examined this opening model by introducing pairs of cysteines into the rat P2X2 receptor that might form disulfide bonds within or between subunits. Receptors were expressed in human embryonic kidney cells, and disulfide formation was assessed by observing the effect of dithiothreitol on currents evoked by ATP. Substitutions in the turret (P90C, P89C/S97C), body wall (S65C/S190C, S65C/D315C) and the transmembrane domains (V48C/I328C, V51C/I328C, S54C/I328C) strongly inhibited ATP-evoked currents prior to reduction with dithiothreitol. Western blotting showed that these channels also formed predominately as dimers and/or trimers rather than monomers. The results strongly support the channel opening mechanism proposed on the basis of available crystal structures.
- Published
- 2014
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- View/download PDF
42. Hans Walter Kosterlitz. 27 April 1903 — 26 October 1996
- Author
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R. Alan North and John Hughes
- Subjects
Gerontology ,medicine.medical_specialty ,Enthusiasm ,business.industry ,media_common.quotation_subject ,Medicine ,General Medicine ,business ,Psychiatry ,media_common ,Endogenous opioid - Abstract
Hans Walter Kosterlitz will be remembered as the winner of an international race to identify the first endogenous opioid, enkephalin. He came to the UK from Berlin in 1934, one of the many scientific émigrés of that period who later so enriched biochemistry, physiology and pharmacology. His entire career was spent at the University of Aberdeen, where he developed a reputation first in carbohydrate metabolism and later in the pharmacology of opiates. His strong experimental skills, and particularly the use of bioassays on tissues from laboratory animals, allowed him to predict the effectiveness and abuse potential of opiate drugs. Most notably, this led to the isolation of the peptide hormone enkephalin, the first of a group of such peptides now often known as endorphins. This work was done in his seventies, after his official retirement, with an energy and enthusiasm that could leave younger colleagues physically tired but intellectually stimulated.
- Published
- 2013
- Full Text
- View/download PDF
43. Neuromodulation by Extracellular ATP and P2X Receptors in the CNS
- Author
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R. Alan North and Baljit S. Khakh
- Subjects
P2Y receptor ,Neuroscience(all) ,Biology ,Neurotransmission ,Synaptic Transmission ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Adenosine Triphosphate ,0302 clinical medicine ,Neuromodulation ,Extracellular ,medicine ,Animals ,Humans ,Receptor ,Ion channel ,030304 developmental biology ,Neurotransmitter Agents ,0303 health sciences ,General Neuroscience ,Brain ,Purinergic signalling ,Cell biology ,medicine.anatomical_structure ,chemistry ,Receptors, Purinergic P2X ,Neuroscience ,Adenosine triphosphate ,030217 neurology & neurosurgery - Abstract
Extracellular adenosine 5′ triphosphate (ATP) is a widespread cell-to-cell signaling molecule in the brain, where it activates cell surface P2X and P2Y receptors. P2X receptors define a protein family unlike other neurotransmitter-gated ion channels in terms of sequence, subunit topology, assembly, and architecture. Within milliseconds of binding ATP, they catalyze the opening of a cation-selective pore. However, recent data show that P2X receptors often underlie neuromodulatory responses on slower time scales of seconds or longer. Herein, we review these findings at molecular, cellular and systems levels. We propose that, while P2X receptors are fast ligand-gated cation channels, they are most adept at mediating slow neuromodulatory functions that are more widespread and more physiologically utilized than fast ATP synaptic transmission in the CNS.
- Published
- 2012
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44. Activation of Trimeric P2X2 Receptors by Fewer than Three ATP Molecules
- Author
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R. Alan North, Olga Stelmashenko, Yue Yang, Vincent Compan, Ulyana Lalo, and Laricia Bragg
- Subjects
DNA, Complementary ,Patch-Clamp Techniques ,Protein subunit ,Biology ,Cell membrane ,chemistry.chemical_compound ,Adenosine Triphosphate ,medicine ,Animals ,Humans ,Biotinylation ,Patch clamp ,Receptor ,Ion channel ,Pharmacology ,Alanine ,Binding Sites ,Lysine ,Cell Membrane ,Articles ,Transmembrane protein ,Rats ,Cell biology ,Protein Subunits ,HEK293 Cells ,medicine.anatomical_structure ,Amino Acid Substitution ,chemistry ,Membrane protein ,Mutation ,Molecular Medicine ,Protein Multimerization ,Adenosine triphosphate ,Receptors, Purinergic P2X2 - Abstract
P2X receptors are trimeric membrane proteins. When they bind extracellular ATP, a conformational change occurs that opens a transmembrane ion channel. The ATP-binding pocket is formed in a cleft between two subunits, and a critical amino acid residue for ATP contact is Lys⁶⁹ (P2X2 numbering). In the present work, we sought to determine whether the binding of fewer than three ATP molecules could open the ion channel. We expressed eight concatenated cDNAs in human embryonic kidney cells, which encoded three serially joined, epitope-tagged, subunits with either Lys or Ala at position 69 (denoted as KKK, KKA, KAK, AKK, KAA, AKA, AAK, and AAA). Western blotting of surface-biotinylated proteins indicated that breakdown of concatemers to individual subunits was minimal. Recording of membrane currents in response to ATP (whole cell and excised outside-out patch) showed that all formed functional channels except AAK, AKA, and AAA. There was no difference in the kinetics of activation and deactivation among KKK, KKA, KAK, and AKK channels, and amplitude of the unitary conductances was in all cases not different from that found after expression of a single wild-type subunit. Currents through KKA and KAK receptors were larger than those observed for AKK receptors. The results indicate that trimeric P2X receptors containing only two intact binding sites can be readily activated by ATP.
- Published
- 2012
- Full Text
- View/download PDF
45. Pannexin 1 forms an anion-selective channel
- Author
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Elizabeth Martin, Alexei Verkhratsky, R. Alan North, Wenxuan Zheng, Vincent Compan, Annmarie Surprenant, and Weihong Ma
- Subjects
Anions ,Cell Membrane Permeability ,Physiology ,Unitary conductance ,Clinical Biochemistry ,Glutamic Acid ,Nerve Tissue Proteins ,Connexins ,Ion Channels ,Cell Line ,Ion ,Chlorides ,Physiology (medical) ,Humans ,Voltage-Dependent Anion Channels ,Receptor ,Aspartic Acid ,Chemistry ,HEK 293 cells ,Conductance ,Human physiology ,Anatomy ,Pannexin ,HEK293 Cells ,Membrane ,Biophysics - Abstract
Pannexin 1 (Panx1) is expressed in various mammalian tissues including the brain and immune cells. Here, we present evidence that Panx1 when expressed in mammalian cells, forms anion-selective channels, with a rank order of permeabilities: NO 3 − > I− > Br− > Cl− > F− ≫ aspartate− ≈ glutamate− ≈ gluconate−. Single-channel Panx1-mediated currents have a unitary conductance around 68 pS. Our results show that Panx1 assembles into a membrane anion channel with a relatively low single-channel conductance.
- Published
- 2012
- Full Text
- View/download PDF
46. Role of P2X4 receptors in synaptic strengthening in mouse CA1 hippocampal neurons
- Author
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R. Alan North, Andrew W. Baxter, Joan A. Sim, and Se Joon Choi
- Subjects
0303 health sciences ,General Neuroscience ,Depolarization ,Long-term potentiation ,Hyperpolarization (biology) ,Neurotransmission ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,BAPTA ,Postsynaptic potential ,Excitatory postsynaptic potential ,Ifenprodil ,Biophysics ,Neuroscience ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
P2X4 receptors are calcium-permeable cation channels gated by extracellular ATP. They are found close to subsynaptic sites on hippocampal CA1 neurons. We compared features of synaptic strengthening between wild-type and P2X4 knockout mice (21–26 days old). Potentiation evoked by a tetanic presynaptic stimulus (100 Hz, 1 s) paired with postsynaptic depolarization was less in P2X4−/− mice than in wild-type mice (230 vs. 50% potentiation). Paired-pulse ratios and the amplitude and frequency of spontaneous excitatory postsynaptic currents (EPSCs) were not different between wild-type and knockout mice. Prior hyperpolarization (ten 3 s pulses to −120 mV at 0.17 Hz) potentiated the amplitude of spontaneous EPSCs in wild-type mice, but not in P2X4−/− mice; this potentiation was not affected by nifedipine, but was abolished by 10 mm 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetra-acetic acid (BAPTA) in the recording pipette. The amplitude of N-methyl-d-aspartate EPSCs (in 6-cyano-7-nitroquinoxaline-2,3-dione, 10 or 30 μm, at −100 mV) facilitated during 20 min recording in magnesium-free solution. In wild-type mice, this facilitation of the N-methyl-d-aspartate EPSC was reduced by about 50% by intracellular BAPTA (10 mm), ifenprodil (3 μm) or 4-(4-fluorophenyl)-2-(4-methylsulphinylphenyl)-5-(4-pyridyl)1H-imidazole (5 μm). In P2X4−/− mice, the facilitation was much less, and was unaffected by intracellular BAPTA, ifenprodil (3 μm) or mitogen-activated protein (MAP) kinase inhibitor 4-(4-fluorophenyl)-2-(4-methylsulphinylphenyl)-5-(4-pyridyl)1H-imidazole (5 μm). This suggests that the absence of P2X4 receptors limits the incorporation of NR2B subunits into synaptic N-methyl-d-aspartate receptors.
- Published
- 2011
- Full Text
- View/download PDF
47. New structure enlivens interest in P2X receptors
- Author
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Liam E. Browne, R. Alan North, and Lin-Hua Jiang
- Subjects
Models, Molecular ,Pharmacology ,Binding Sites ,Protein Conformation ,Receptors, Purinergic P2 ,Glutamate receptor ,Review ,Plasma protein binding ,Biology ,Toxicology ,Permeability ,Cell biology ,Adenosine Triphosphate ,Metabotropic receptor ,Nicotinic agonist ,Receptors, Purinergic P2X ,Mutagenesis, Site-Directed ,Biophysics ,Animals ,Humans ,Receptor ,Ion channel ,Protein Binding ,Acetylcholine receptor ,Ionotropic effect - Abstract
P2X receptors are ATP-gated membrane ion channels with multifarious roles, including afferent sensation, autocrine feedback loops, and inflammation. Their molecular operation has been less well elucidated compared with other ligand-gated channels (nicotinic acetylcholine receptors, ionotropic glutamate receptors). This will change with the recent publication of the crystal structure of a closed P2X receptor. Here we re-interpret results from 15 years of experiments using site-directed mutagenesis with a model based on the new structure. Previous predictions of receptor stoichiometry, the extracellular ATP binding site, inter-subunit contacts, and many details of the permeation pathway fall into place in three dimensions. We can therefore quickly understand how the channel operates at the molecular level. This is important not only for ion- channel aficionados, but also those engaged in developing effective antagonists at P2X receptors for potential therapeutic use.
- Published
- 2010
- Full Text
- View/download PDF
48. The birth and postnatal development of purinergic signalling
- Author
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Alexei Verkhratsky, R. A. North, Bo Fredholm, and Geoffrey Burnstock
- Subjects
Co transmission ,Physiology ,Extramural ,Immunology ,Signalling system ,Purinergic receptor ,Biology ,Signal transduction ,Purinergic signalling ,Guinea pig ileum ,Neuroscience - Abstract
The purinergic signalling system is one of the most ancient and arguably the most widespread intercellular signalling system in living tissues. In this review we present a detailed account of the early developments and current status of purinergic signalling. We summarize the current knowledge on purinoceptors, their distribution and role in signal transduction in various tissues in physiological and pathophysiological conditions.
- Published
- 2010
- Full Text
- View/download PDF
49. Ectodomain Lysines and Suramin Block of P2X1 Receptors*
- Author
-
R. Alan North, Helen Broomhead, and Joan A. Sim
- Subjects
Patch-Clamp Techniques ,Suramin ,Lysine ,Molecular Sequence Data ,Biology ,Kidney ,Biochemistry ,Cell Line ,Mice ,Molecular Basis of Cell and Developmental Biology ,Extracellular ,medicine ,Animals ,Humans ,heterocyclic compounds ,Patch clamp ,Amino Acid Sequence ,Receptor ,Molecular Biology ,Peptide sequence ,Sequence Homology, Amino Acid ,Receptors, Purinergic P2 ,Benzenesulfonates ,Antagonist ,Muscle, Smooth ,Cell Biology ,Molecular biology ,Protein Structure, Tertiary ,Ectodomain ,Receptors, Purinergic P2X ,medicine.drug - Abstract
P2X(1) receptors belong to a family of cation channels gated by extracellular ATP; they are found inter alia in smooth muscle, platelets, and immune cells. Suramin has been widely used as an antagonist at P2X receptors, and its analog 4,4',4'',4'''-[carbonylbis(imino-5,1,3-benzenetriylbis(carbonylimino))] tetrakis-benzene-1,3-disulfonic acid (NF449) is selective for the P2X(1) subtype. Human and mouse P2X(1) receptors were expressed in human embryonic kidney cells, and membrane currents evoked by ATP were recorded. ATP (10 nm to 100 microm) was applied only once to each cell, to avoid the profound desensitization exhibited by P2X(1) receptors. Suramin (10 microm) and NF449 (3-300 nM) effectively blocked the human receptor. Suramin had little effect on the mouse receptor. Suramin and NF449 are polysulfonates, with six and eight negative charges, respectively. We hypothesized that species differences might result from differences in positive residues presented by the large receptor ectodomain. Four lysines in the human sequence (Lys(111), Lys(127), Lys(138), and Lys(148)) were changed individually and together to their counterparts in the mouse sequence. The substitution K138E, either alone or together with K111Q, K127Q, and K148N, reduced the sensitivity to block by both suramin and NF449. Conversely, when lysine was introduced into the mouse receptor, the sensitivity to block by suramin and NF449 was much increased for E138K, but not for Q111K, Q127K, or N148K. The results explain the marked species difference in antagonist sensitivity and identify an ectodomain lysine residue that plays a key role in the binding of both suramin and NF449 to P2X(1) receptors.
- Published
- 2008
50. Molecular Shape, Architecture, and Size of P2X4 Receptors Determined Using Fluorescence Resonance Energy Transfer and Electron Microscopy*
- Author
-
James A Fisher, Robert C. Ford, Baljit S. Khakh, Samuel J. Fountain, R. Alan North, and Mark T. Young
- Subjects
Models, Molecular ,Time Factors ,Protein Conformation ,Green Fluorescent Proteins ,Lipid Bilayers ,Single particle analysis ,Q1 ,Biochemistry ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Protein structure ,Lectins ,Microscopy ,Fluorescence Resonance Energy Transfer ,Humans ,Lipid bilayer ,Molecular Biology ,Ion channel ,030304 developmental biology ,Ions ,0303 health sciences ,Chemistry ,Receptors, Purinergic P2 ,Resolution (electron density) ,Cell Biology ,Protein Structure, Tertiary ,Crystallography ,Membrane Transport, Structure, Function, and Biogenesis ,Microscopy, Electron ,Förster resonance energy transfer ,Ectodomain ,Carrier Proteins ,Receptors, Purinergic P2X4 ,030217 neurology & neurosurgery ,Protein Binding - Abstract
P2X receptors are ATP-gated nonselective cation channels with important physiological roles. However, their structures are poorly understood. Here, we analyzed the architecture of P2X receptors using fluorescence resonance energy transfer (FRET) microscopy and direct structure determination using electron microscopy. FRET efficiency measurements indicated that the distance between the C-terminal tails of P2X(4) receptors was 5.6 nm. Single particle analysis of purified P2X(4) receptors was used to determine the three-dimensional structure at a resolution of 21A; the orientation of the particle with respect to the membrane was assigned by labeling the intracellular C termini with 1.8-nm gold particles and the carbohydrate-rich ectodomain with lectin. We found that human P2X(4) is a globular torpedo-like molecule with an approximate volume of 270 nm(3) and a compact propeller-shaped ectodomain. In this structure, the distance between the centers of the gold particles was 6.1 nm, which closely matches FRET data. Thus, our data provide the first views of the architecture, shape, and size of single P2X receptors, furthering our understanding of this important family of ligand-gated ion channels.
- Published
- 2008
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