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To analyze a case of acute retinal necrosis (ARN) associated with pseudorabies virus (PRV) infection and discusses the clinical characteristics of PRV-induced ARN (PRV-ARN). Case report and literature review of ocular features in PRV-ARN. A 52-year-old female diagnosed with encephalitis presented with bilateral vision loss, mild anterior uveitis, vitreous opacity, occlusive retinal vasculitis, and retinal detachment in her left eye. The result of metagenomic next-generation sequencing (mNGS) indicated that both cerebrospinal fluids and vitreous fluid tested positive for PRV. PRV, a zoonosis, can infect both humans and mammals. Patients affected with PRV may experience severe encephalitis and oculopathy, and the infection has been associated with high mortality and disability. ARN is the most common ocular disease, which develops rapidly following encephalitis and is characterized by five figures: bilateral onset, rapid progression, severe visual impairment, poor response to systemic antiviral drugs, and an unfavorable prognosis. [ABSTRACT FROM AUTHOR]

This paper develops a novel processing method of plasma electrolytic oxidation-assisted micromilling (PEOAM). Electrolyte solutions with KH2PO4, NaAlO2, or Na2SiO3 as the main component are designed, and three types of oxide films are grown on the surface of a Ti6Al4V alloy in situ by means of plasma electrolytic oxidation. The morphology and composition of the oxide films are characterized by scanning electron microscope and energy dispersive spectrum. Additionally, the cutting force and surface roughness of PEOAM are measured by dynamometer and white light interferometer, respectively. A comparison between PEOAM and conventional micromilling in terms of cutting force, tool wear, chips, and surface roughness is conducted, with results showing that oxide films with about 20 μm thickness are loose and porous, their hardness decreasing to a minimum of 1.12 GPa, which corresponds to 23.3% of the original hardness value. At the axial cutting depth of 18 μm, compared to the Fx, Fy, and Fz values of the Ti6Al4V alloy substrate, the average milling forces of the NaAlO2 oxide film are the most significantly reduced (35.1%, 15.7%, and 94.8% of the original values, respectively). At the axial cutting depth of 25 μm, the surface roughness (Ra) value of PEOAM is reduced by 0.08–0.12 μm. Consequently, under the same cutting parameters, PEOAM can effectively reduce the cutting force, prolong the service life of the tool, and improve surface quality. [ABSTRACT FROM AUTHOR]

Purpose: To investigate the ocular toxicity and pharmacokinetics of intrastromal injection of amphotericin B (AmB) in a rabbit model. Methods: Forty albino rabbits were randomly divided into five groups (eight per group). The rabbits were anesthetized before they received the medication. Intrastromal injection of 0.1 ml balanced salt solution containing 0, 5, 10, 20 or 30 μg of AmB was performed on eyes of each group five times (once per four days), respectively. The presence of possible corneal clouding, epithelial erosion and corneal neovascularization was monitored with a slit-lamp biomicroscope. Corneal ultrasonic pachymetry was used to detect the corneal thickness of intrastromal-injected eyes. Thirty days after the last injection, the corneal transparency as well as the number and ultrastucture of corneal endothelial cells were examined. The concentrations of the AmB in the cornea and aqueous humor were evaluated at 30 min, 6 h and at 1, 3 and 7 days after the intrastromal injection of 10 µg AmB. Results: Intrastromal injection of AmB at concentrations of 5 and 10 μg per 0.1 ml did not induce obvious toxicity to the cornea when compared with the controls. However, when the concentration of AmB increased to 20 μg per 0.1 ml or more, corneal edema, corneal epithelial erosion and severe neovascularization appeared. A single intrastromal injection of 10 μg AmB achieved an effective drug level in corneas which was maintained for up to 7 days. Conclusions: Intrastromal injection of AmB at a concentration of less than10 μg per 0.1 ml is safe to the rabbit corneas. Intrastromal injection of AmB may be an adjunctive treatment for deep recalcitrant fungal keratitis. [ABSTRACT FROM AUTHOR]

Retinal progenitor cells (RPCs) have a potential role in the treatment of retinal degenerative diseases. This study is to investigate in vitro and in vivo characteristics and retinal transplantation of RPCs cultured in media with or without serum. Progenitor cells obtained from the neural retina of human eyes at 6-16 weeks gestation were cultured in serum-free media (SF-hRPCs) or in media containing 10% fetal bovine serum (FBS) (S-hRPCs). The differences were characterized between the cells cultured in vitro and transplanted (retinal transplantation) into Royal College of Surgeons (RCS) rats. The functional status of the rats was examined by flash-electroretinogram recordings. The result was that S-hRPCs exhibited higher proliferative dynamics in vitro. On the basis of outer nuclear layer thickness and flash-electroretinograms, S-hRPCs were more efficacious in slowing the progression of retinal degeneration following transplantation compared with SF-hRPCs. Moreover, retinal mesenchymal-like stem cells were isolated and identified from the S-hRPCs cultures. Our study demonstrated the potential of retinal MSCs for the treatment of retinal degeneration., Competing Interests: None., (IJCEP Copyright © 2018.)

Background: Little is known about the ocular penetration following intrastromal or intracameral injection of amphotericin B (AMB), the current drug of choice in fungal keratitis. Concentrations of AMB were investigated in the cornea and aqueous humor of rabbits after using one of three different routes of administration: topical 0.25% AMB eye drops and intrastromal and intracameral injection of AMB (10 microg)., Methods: Forty-five healthy rabbits were randomly divided into 3 groups. The eyes of group A and group B received a 0.1-ml intrastromal and intracameral injection, respectively, containing 10 microg AMB. Group C received topical 0.25% AMB (corneal epithelium debrided, every 5 min for 30 min). Cornea and aqueous humor concentrations of AMB after 30 min, 6 h, 1, 3 and 7 days were analyzed by high-performance liquid chromatography., Results: After a single injection, effective drug levels were achieved in corneas in group A, maintained for 7 days, exceeding the minimum inhibitory concentration at which 90% of isolates are inhibited (MIC(90)) for a wide spectrum of fungi and molds. There were significant differences (p < 0.001) compared with group B and group C. Effective drug levels were achieved in the aqueous humor in group B at 30 min after a single injection, exceeding MIC(90), but drug levels decreased abruptly within 1 day. There were significant differences (p < 0.004) compared with group A and group C, and a considerable amount of AMB was detected in corneas and aqueous in group C within 1 day., Conclusion: High drug levels can be reached that cover the MICs of most fungi in the rabbit cornea and aqueous humor after intrastromal and intracameral injection, respectively. Penetration of topical AMB greatly increased after epithelial abrasion., (Copyright 2009 S. Karger AG, Basel.)