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Supplementary Material 1: Table S1. Distribution of genotypes of tSNPs in cases and controls

Supplementary Material 3: Table S3. Association between the 40 tSNPs in 4 genes and AIDS clinical stages

Supplementary Material 2: Table S2. Distribution of haplotypes of Chk1 and Cdc25C gene in cases and controls

Supplementary Material 4: Table S4. Association between the tSNPs and HIV-1 infection susceptibility in different subgroups divided by CD4+ T cell counts

Supplementary Material 5: Table S5. Gene-gene interaction models, as identified by GMDR

For non-directional drilling cores, selection of samples and the test methods for in situ stress measurements to evaluate the Kaiser effect (KE) were proposed, and the magnitude and direction of the principal stresses were derived from first principles. Based on this approach, the KE for 423 samples in the Burtai and Baode coal mines in the northern Ordos Basin (NOB), China, have been investigated. The results show that the maximum horizontal principal stress (σH), the minimum horizontal principal stress (σh) and the vertical stress (σv) varied with depth and location, and the values increase with increasing depth. Generally, the horizontal stresses play a leading role. For the main stress regimes in the NOB, σH > σh > σv (Burtai Mine, σH > σv > σh (Burtai Mine, 170–800 m; Baode Mine, 170–400 m), and the σv > σH > σh stress regimes are mainly distributed in moderately deep to deep coal mines. For rock masses with a depth of 350 m, k ((σH + σh)/2σv) tends to 1, indicating that a deep critical state will gradually emerge. The test results are compared with those for the overcoring (OC) method, the anelastic strain recovery (ASR) method and micro-hydraulic fracturing (HF). The relative errors for σH, σh and σv were 14.90%, 19.67%, 15.47% (Burtai Mine) and 10.74%, 22.76%, 19.97% (Baode Mine), respectively, and the errors are all within an acceptable range, thus verifying the reliability of the KE method. The dominant orientation for the σH (Burtai mine, NE-NNE; Baode Mine, NEE) is obtained via paleomagnetic technology, and the data are consistent with those (NE-NEE) of the earthquake focal mechanism solutions for the area. Based on the Byerlee–Anderson theory, a discussion is given on the levels of stress accumulation in the rock mass of the mines. For dry rocks or hydrostatic pressure rocks, the friction coefficients of the faults are low for both locations, and the values are less than the lower limit (0.6) of the strike-slip faults slip, indicating that the stress fractures at a low level around the study areas are lower than the friction limit stress. The stress accumulation levels in the Baode Mine are slightly larger than those in the Burtai Mine.

After coal is treated by thermal solution of solvent, a certain amount of thermal solution oil and residue can be obtained, and the macromolecular network structure in coal can also be relaxed. These will inevitably affect the emission of harmful gases and distribution of the pore structure when the residue is made into activated carbon (AC). In this paper, the effects of thermal solution pretreatment on the microcrystalline structure, surface properties, pore structure of resultant ACs at different temperatures, and their catalytic performances in methane decomposition to hydrogen were investigated. The results show that the surface oxygen-containing functional groups of the residue-based ACs are changed, and the specific area of ACs increases from 1730 to 2652 m2/g with the increase in activated temperature. The pore diameter distribution of ACs also is changed. In the process of methane decomposition to hydrogen, the residue-based ACs show higher catalytic activity than coal-based ACs. AC-1123-1 and AC-1123 show the best stability, while AC-823-1 has the highest initial activity. With the increase in activated temperature, residue-based ACs show higher activity and stability, and partial fibrous carbon is deposited on the surface of ACs after the reaction. It is thought that increased mesoporosity is beneficial to the catalytic activity and stability of AC in methane decomposition to hydrogen, and the reduction of surface oxygen-containing functional groups contribute to the formation of fibrous carbon on the surface of AC after the reaction.

Additional file 2: Supplementary Table 1. Independent sample test of age.

Additional file 1: Supplementary Figure 1. Genotyping feasibility assessment of candidate SNPs. Priority "Fail" was not genotyped, and one of the linkage SNPs (red) was selected for genotyping. Supplementary Figure 2. P-P plot shows that the age is normally distributed. The data points all fall on the obliqueline of 45°, showing strong normality. Supplementary Figure 3. Association analysis of 14 SNPs with clinical test indexes in HCC patients. (A) Association analysis of 14 SNPs with AFP in HCC patients. (B) Association analysis of 14 SNPs with AST in HCC patients. (C) Association analysis of 14 SNPs with ALT in HCC patients. (D) Association analysis of 14 SNPs with AST/ALT ratios in HCC patients. Model 1 is the TCTTGC/TCTTGC+TCTTGC/T genetic model of rs10589312. Model 2 is the CC+C/CA genetic model of rs11438260. The values in red italics are statistically significant. The size of the indicator is represented by the median value (inter quartile range).

Background Most the HCC susceptible loci identified by GWAS are located in non-coding regions, and the mechanism of action remains unclear. The objective of this study was to explore the relationship among SNPs on lncRNA that affects ceRNA mechanism and the risk and prognosis of HCC. Methods In this study, combined with multiple databases, eight lncRNA genes that affect HCC were systematically screened through the mechanism of lncRNA-mediated ceRNA, and 15 SNPs that affect miRNA binding in the eight lncRNA genes were annotated. Genotyping was conducted in 800 HCC cases and 801 healthy controls from a Han population in Northeast China to examine the genetic risk associated with HCC. Results The GG, GC and GG + GC genotypes of HOTAIR rs7958904 were found to be associated with a 0.65, 0.59 and 0.63-fold decreased HCC risk, respectively. Moreover, in the stratified analysis of clinical characteristics, HCC patients with PVT1 rs3931282 AA + GA genotypes were less prone to develop late-stage tumors. When stratified by clinical biochemical indexes, rs1134492 and rs10589312 in PVT1 and rs84557 in EGFR-AS1 showed significant associations with AST, ALT or AST/ALT ratio. In addition, the potential ceRNA regulatory axes might be affected by the five positive SNPs were constructed to explain the causes of these genetic associations. Conclusions This first study demonstrated the association of SNPs on lncRNA -mediated ceRNAs with HCC. The results provide new insights of how non-coding loci confer the interindividual differences to susceptibility and progression of HCC.

For non-directional drilling cores, the sample selection and test method for Kaiser effect (KE) in-situ stress measurement were proposed, and the magnitude and direction of its principal stresses were theoretically derived. Based on this method, the KE of 423 samples in Burtai and Baode coal mines in northern Ordos Basin (NOB) were tested. The results show that σH, σh and σv vary with depth and location, and their values increase with increasing of depths. Generally, horizontal stresses play a leading role. There are main stress regimes in NOB: σH > σh > σv (Burtai, σv > σh (Burtai, 170-800 m; Baode, 170-400 m), and the σv > σH > σh stress regime is mainly distributed in moderately deep to deep coal mines. For rock masses with a depth of 350m, k ((σH + σh) / 2σv) tends to 1, indicating that deep critical state will gradually emerge. The test results were compared with those of overcoring method (OC), elastic strain recovery (ASR) and micro-hydraulic fracturing (HF). The relative errors of σH, σh and σv are 14.90%, 19.67%, 15.47% (Burtai) and 10.74%, 22.76%, 19.97% (Baode), and they are all within a reasonable range required by the project, which verifies the reliability of KE method. The dominant orientation of σH (Burtai, NE-NNE; Baode, NEE) was obtained by using paleomagnetic technology, which is consistent with that (NE-NEE) of earthquake focal mechanisms in this area. Based on Byerlee-Anderson theory, the stress accumulation level of mine rock mass was discussed. Under dry rocks or hydrostatic pressure rocks, the friction coefficient of faults is both low, which is less than the lower limit (0.6) of strike-slip faults slip, indicating that the fracture stress with a low level around the study area is lower than the friction limit stress. The stress accumulation level in Baode mine is slightly larger than that in Burtai mine.