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Context: Cellular abnormalities in the ducts and breast tissue cause breast cancer to invade nearby tissues like the liver. The fibroblast growth factor (FGF) signals are sent to its receptor and regulate various cellular processes to maintain liver homeostasis. However, this condition has few and inefficient treatments. Breast cancer is characterized by the development of cellular abnormalities within the ducts and breast tissue, leading to the invasion of adjacent tissues, such as the liver. Aims: To examine the impact of Rhodomyrtus tomentosa administration on the expression of the FGF family in cancer animal models, with a specific focus on the liver. Methods: Cancer model rats were administered at dosages of 100, 200, and 300 mg/kg BW for 30 days. Liver tissue and blood serum samples were extracted from the rat. Liver tissue was stained for immunohistochemistry using FGF1, FGF15, FGF19, and FGF21, and blood samples were collected for ELISA analysis. Results: The study found that DMBA in hepatocyte cells degrades parenchyma, making it hydrophilic and necrosating. Hepatocyte cell function improved with the greatest dose of R. tomentosa. The elevation was associated with an area around the portal vein where recently dividing hepatocytes form clusters. Conclusions: The promise of R. tomentosa as a candidate for the development of hepatoprotective medicines in cancer treatment arises from its ability to influence the expression of crucial liver health markers, including FGF1, FGF15, FGF19, and FGF21.

Cancer refers to the proliferation and multiplication of aberrant cells inside the human body, characterized by their capacity to proliferate and infiltrate various anatomical regions. Numerous biochemical pathways and signaling molecules have an impact on the cancer auto biogenesis process. The regulation of crucial cellular processes necessary for cell survival and proliferation, which are triggered by phytochemicals, is significantly influenced by signaling pathways. These pathways or components are regulated by phytochemicals. Medicinal plants are a significant reservoir of diverse anticancer medications employed in chemotherapy. The anticancer effects of phytochemicals are mediated by several methods, including induction of apoptosis, cessation of the cell cycle, inhibition of kinases, and prevention of carcinogenic substances. This paper analyzes the phytochemistry of seven prominent plant constituents, namely, alkaloids, tannins, flavonoids, phenols, steroids, terpenoids, and saponins, focusing on the involvement of the MAPK/ERK pathway, TNF signaling, death receptors, p53, p38, and actin dynamics. Hence, this review has examined a range of phytochemicals, encompassing their structural characteristics and potential anticancer mechanisms. It has underscored the significance of plant-derived bioactive compounds in the prevention of cancer, utilizing diverse molecular pathways. In addition, this endeavor also seeks to incentivize scientists to carry out clinical trials on anticancer medications derived from plants.

In this research has been conducted on manufacturing ceramic materials based on Belanda Teak wood powder and bentonite using Solid State Reaction method. The composition variation of Belanda Teak wood powder with bentonite is 4:5, 5:5 and 6:5, then activated at 1000°C. Then, mixed using ball milling at 500 rpm for 30 min. Density analysis was conducted to determine the density. The density analysis obtained was 2.20 gr/cm3, 2.32 gr/cm3, and 2.33 gr/cm3 for samples 4:5, 5:5, and 6:5. The characterization analysis was SEM-EDX, XRD, and DTA. The results of XRD analysis obtained a hexagonal crystal structure. The size of the crystal diameter will increase as the teak wood powder mass ratio decreases in each sample. SEM-EDX results obtained morphology in the 4:5 composition is better than the 5:5 and 6:5 samples, and the optimum composition spectrum is 6:5, which has a maximum weight and atomic percentage and there are no other elements mixed beside the main elements. The DTA results stated that the sample phase start from 95.05°C, and there is an endothermic peak at a temperature of 427.54 °C with a mass loss of 9.14 mg and there is an increase in temperature to 534.29 °C with a mass loss of 4.34 mg due to recrystallization. Based on the results of this research, these materials can be recommended to become making ceramics materials.

Polyscias scutellaria (PS), an increasingly recognized botanical marvel, has emerged as a remarkable reservoir of natural antioxidants and anti-inflammatory compounds, holding immense potential for enhancing health and promoting overall well-being. In this comprehensive investigation, we meticulously examined the multifaceted properties of PS through various parameters, including DPPH scavenging activity, total phenol, and total flavonoid content in its ethanol extract (EEPS), ethyl acetate extract (EAPS), and n-hexane extract (nhPS). Additionally, we conducted in-depth assessments of cellular responses to EEPS and EAPS, encompassing cell viability, nitric oxide (NO) production, and the modulation of pivotal pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and interleukin-12 (IL-12), utilizing the RAW 264.7 cell line as a model system.Our findings illuminate the exceptional antioxidant prowess of PS extracts, with EEPS, EAPS, and nhPS displaying noteworthy DPPH scavenging activities. These results underscore their potential in quenching harmful free radicals and mitigating oxidative stress. Furthermore, our investigation unveils the promising anti-inflammatory attributes of EEPS and EAPS, as evidenced by their capacity to preserve cellular viability, dampen NO production, and suppress the secretion of critical pro-inflammatory mediators (TNF-α, IL-6, IL-1β, and IL-12) in RAW 264.7 cells. These remarkable anti-inflammatory effects hint at the therapeutic potential of PS in ameliorating chronic inflammatory conditions and bolstering the body’s immune response.In conclusion, Polyscias scutellaria stands as an emerging botanical champion, offering a wealth of natural antioxidants and anti-inflammatory compounds that hold great promise for optimizing health and well-being. This study opens exciting avenues for future research to elucidate the precise bioactive constituents within PS and unravel their intricate mechanisms of action, paving the way for the development of innovative therapeutic interventions and wellness-enhancing products. The remarkable properties of PS underscore its potential as a cornerstone of holistic health and a valuable asset in the pursuit of a vibrant and balanced life. Graphical abstract

This study explores the chronic toxicity of nano-formulated Bischofia javanica leaves, a plant with pharmacological significance in Indonesia, following OECD guidelines 452. Graded doses (2, 4, 6, and 8 g/kg BW) of nano-formulated Bischofia javanica were administered to the treatment groups for 60 days, while the control group (K-) received only water. On the 61st day, the mice were euthanized, and samples for biochemical, hematological, and histopathological analysis were collected. Statistical analysis involved one-way ANOVA and Tukey’s post hoc tests. Graded doses of nano-formulated Bischofia javanica leaves caused significant alterations in liver function. Doses between 2 and 4 g/kg BW improved liver histology and physiological markers. Higher doses (6 to 8 g/kg BW) led to liver dysfunction and histological degeneration, characterized by substantial fatty alterations, intracellular fat accumulation in hepatocytes, and compromised central blood vessels and sinusoids. The lungs showed signs of alveolar inflammation, epithelial exfoliation, debris, and the accumulation of inflammatory cells in alveolar spaces. Lower doses showed uniformly distributed cardiac blood vessels, while higher doses resulted in heart hemorrhages and amorphous exudates. Except for high doses, which significantly elevated specific liver damage indicators, no other levels of Bischofia javanica caused treatment-related mortality or significant alterations in hematological and biochemical parameters. Blood sugar levels remained stable across the dose range. In conclusion, moderate doses of nano-formulated Bischofia javanica leaf extracts can enhance physiological functions positively. However, caution is necessary when contemplating high doses, as they can cause dysfunction or damage vital organ systems.

The use of the chemotherapeutic agent doxorubicin is limited due to its potential to cause signifi-cant hepatorenal damage. The present study aimed to investigate the potential hepatoprotective and nephroprotective effects of Vernonia amygdalina, a medicinal plant with known antioxidant and anti-inflammatory properties, against doxorubicin-induced toxicity in rats. Male Wistar rats were randomly divided into four groups: Control, Doxorubicin (DOX), DOX + Vernonia Amyg-dalina (DOX+VA), and Vernonia amygdalina (VA) alone. DOX and DOX+VA groups were treated with a single intraperitoneal injection of doxorubicin (15 mg/kg body weight). The DOX+VA group received Vernonia amygdalina extract (100, 300, 500 mg/kg body weight) by oral gavage for 14 days following doxorubicin injection. The results demonstrated that Vernonia amygdalina significantly reduced the elevated levels of liver and kidney function biomarkers, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatinine, induced by doxorubicin. The histological examination of the liver and kidney tis-sues also confirmed the protective effects of Vernonia amygdalina against doxorubicin-induced damage. Furthermore, Vernonia amygdalina treatment was found to mitigate oxidative stress by restoring the levels of glutathione (GPx), Catalase, NO and SOD and decreasing the level of malondialdehyde (MDA) in liver and kidney tissues. Additionally, Vernonia amygdalina sig-nificantly suppressed the renal injury markers, NGAL, cystatin-c, KIM-1, and NAG. In conclu-sion, the results of this study suggest that Vernonia amygdalina has potent hepatoprotective and nephroprotective effects against doxorubicin-induced toxicity in rats. These protective effects are mediated by its antioxidant, and free radical scavenging properties. Further investigation is needed to determine the potential clinical relevance of Vernonia amygdalina in protecting against the hepatorenal damage induced by doxorubicin in human subjects. Graphical abstract:

Cardiovascular disorder is the leading cause death in the world, one of them are acute myocardial infarction (AMI) which associated with hypertension and cardiac remodeling. ISO may cause inflammation, enhance the production of oxidative stress while decrease the antioxidant defensive system, myocardium impairment, calcium overload, enhanced cyclic adenosine monophosphate level, intracellular acidosis, and altered membrane permeability. Vernonia amygdalina (VA) is a medicinal plant with antioxidant and anti-inflammatory properties. This study investigated the potential cardioprotective effect of VA on ISO-induced cardiac toxicity in rats. Male Wistar rats were randomly divided into six groups: ISO (ISO), quercetin 100 mg/kg plus ISO (ISO+QR), VA ethanol extract 100, 300, 500 mg/kg plus ISO (ISO+VA100, ISO+VA300 and ISO+VA500). ISO was administered subcutaneously (85 mg/kg) on days 15 while quercetin and VA extract and was given orally for 14 days. At the end of the experiment, the blood was taken from the heart were analyzed for markers of cardiac, oxidative stress and inflammation. The ISO group exhibited significant (p

Context: Swimming is a common form of exercise; however, excessive exercise might reduce sperm count by lowering testosterone levels and increasing the production of free radicals, commonly known as reactive oxygen species (ROS). In Indonesia, Vitis gracilis Wall. is a traditional remedy for increasing stamina. Aims: To assess the concentration of spermatozoa after vigorous physical activity and V. gracilis administration, as well as the histological and apoptotic changes in testicular histology that occur via caspase-3 expression. Methods: This study was conducted on six groups of rats: the control group (G+), a group of rats subjected to vigorous swimming then administered 0.2 mg/kg BW vit C (GVitC), and three groups of rats subjected to vigorous swimming then administered 100, 125, or 150 mg/kg BW V. gracilis (G100, G125, and G150). Testicular tissue and blood serum samples were extracted from the rats subjected to vigorous swimming. Testicular tissue was immunohistochemically stained using caspase-3 antibody and TUNEL assays, while blood samples were analysed using ELISA. Results: V. gracilis administration significantly affected IL-6 and testosterone levels (p

Extracts of Phaleria macrocarpa have been used for years in traditional medicine and have been evaluated scientifically as well. This study aimed to evaluate the chronic toxicity level of nano herbal Phaleria macrocarpa and its effect on the changes in hematology, biochemistry, electrolytes, and organ histopathology. High Energy Milling (HEM) produced nano herbal Phaleria macrocarpa. By giving nano herbal Phaleria macrocarpa at doses of 300, 600, and 900 mg/kg BW for 56 days, the mice’s blood was collected for the hematological and electrolyte parameters. The kidney and liver were assessed for biochemical parameters. As a result, the nano herbal Phaleria macrocarpa affected the histopathological organs, hematological, biochemical, and electrolyte parameters at an appropriate dose of 300–600 mg/kg BW. The maximum period of the administration this herbal medicine is one month. Therefore, with the correct dose and period of administration, this plant can be used as herbal medicine in the future.

The prevalence of breast cancer among patients in Indonesia is significant. Indonesian individuals maintain the belief that cancer cannot be cured alone by pharmaceuticals and treatment; herbal remedies must be used in conjunction. Rhodomyrtus tomentosa, also known as Haramonting, is an indigenous Indonesian medicinal plant renowned for its copious antioxidant properties. The objective of study was to assess the impact of haramonting on breast cancer by examining the expression of various biomarker proteins associated with breast cancer. Haramonting was administered to breast cancer model mice at different doses over a period of 30 days. Subsequently, blood and breast samples were obtained for immunohistochemistry and enzyme-linked immunosorbent assays (ELISA). Authors have discovered that there has been a notable rise in the proliferation of epithelial cells in the duct lobes, resulting in the formation of ducts and lobules. Additionally, the researchers discovered that the breasts exhibited distinct clinical and histological alterations. Haramonting possesses the capacity to restore the concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) to normal levels in the blood serum of rats afflicted with cancer. The histopathological analysis of the breast tissue revealed elevated levels of Her2, IL33, EGFR, and MUC1. The authors also discovered a notable increase in the growth of epithelial cells, with two or more layers of cells reaching towards the centre of the duct. The size of the epithelial cells exhibits variability; however, this state ameliorates with the administration of a dosage of 300 mg/kgBW of this botanical specimen. This study proposes that Haramonting may be effective in treating breast cancer.

Inhaling Allethrin (C19H26O3) may induce oxidative stress in lung cells by causing the formation of free radi-cals. Interleukins (IL) are a group of secreted cytokines or proteins and signaling molecules initially produced as an immune response by leukocytes. Rhodomyrtus tomentosa (Aiton) Hassk. (haramonting) contains antioxidants that may prevent lung damage induced by allethrin-containing electric mosquito repellents. In this study, six groups of rats were exposed to allethrin via an electric mosquito repellent, including positive, negative, and comparison control groups and three groups were administered Rhodomyrtus tomentosa (Aiton) Hassk at 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. After 30 days, the pulmonary tissue and the blood were taken for immunohisto-chemical and ELISA analysis. The accumulation of inflammatory cells causes the thickening of the alveolar wall structures. Injuries were more prevalent in the A+ group than in the other groups. The connection between the alveoli and blood capillaries, which can interfere with alveolar gas exchange, is not regulated, and the lu-minal morphology is aberrant, causing damage to the alveolar epithelial cells. Exposure to electric mosquito coils containing allethrin can increase the expression of interleukin-1, interleukin-8, interleukin-9, and interleu-kin-18 in blood serum and tissues while decreasing the expression of interleukin-6 and interleukin-10. Like the Vitamin C group, Rhodomyrtus tomentosa can increase alveolar histological alterations by decreasing the ex-pression of IL-1β, IL-8, IL-9, and IL-18 while increasing IL-6 and IL-10. So that this plant can be developed in the future as a drug to prevent lung harm from exposure.

Context: The high mortality rate in people with lung disease indicates that this disorder requires immediate attention. It is established that excessive swimming can reduce the ability of lung cells to function and cause respiratory problems. Vitis gracilis Wall. is a traditional medicinal plant used by the Karo people of North Sumatra to improve stamina. Aims: To analyze apoptosis via cytochrome c in serum and lung tissue after V. gracilis administration. Methods: Rats that experienced excessive physical activity received six different treatments: two groups as negative and positive control groups, one vitamin C control group, and three groups orally administered with ethanolic extract of V. gracilis leaves at different doses. Immunohistochemistry, ELISAs, and TUNEL assays were used to assess study parameters. Results: There were significant differences (p

Context: Rhodomyrtus tomentosa has the potential to contain compounds that enhance health. One type of disease that requires antioxidants in its healing is cancer. R. tomentosa extract was resized to nano size to optimize cell permeability. Aims: To analyze the effects of micro-colloidal leaves extract of R. tomentosa (MR) on MMP9, GLUT-1, and IL-1β expression in cervical cancer. Methods: Wistar rats were divided into five treatments, namely untreated (C-), cervical cancer (C+), and a cervical cancer rat model that was given R. tomentosa extract (MR) 100 mg/kg (MR100), 200 mg/kg (MR200), and 400 mg/kg (MR400). After receiving the extract for 30 days, all groups were analyzed using an immunohistochemical technique to detect MMP9, GLUT-1, and IL-1β antibodies. Results: From the statistical analysis, there was a substantial difference in the expression of MMP9, GLUT-1, and IL-1 in the C+ group compared to the C-, MR100, MR200, and MR400 groups (p

Context: Excessive swimming can reduce the ability of hippocampus cells and memory function. When hippocampus volume is lost in late adulthood, aerobic exercise can help recover it. This improvement in memory performance is beneficial, but excessive aerobic exercise can harm the hippocampal structure. Vitis gracilis Wall. is a traditional medicinal plant of North Sumatra’s Karo people that works well as a stamina booster. Aims: To analyze apoptosis via cytochrome c in serum and brain tissue (hippocampal cells) after oral administration of V. garcilis. Methods: Six treatments were given to rats that were given excessive physical exercise consisting of G-: negative control; G+: Swim rats; G.Vit.C: Swim rats +Vitamin C 0.02 mg/kg BW; G100: Swim rats + V. gracilis 100/kg, G125; Swim rats + V. gracilis 125 mg/kg; G150: Swim rats + V. gracilis 150 mg/kg. Immunohistochemistry, ELISA, and Tunel Assay were used to assess study parameters. Results: There were significant differences (p

Cervical carcinoma is one of the most common malignant carcinomas around the world, including Indonesia. Rhodomyrtus tomentosa is an herbal medicine that is often used in Asia as a therapeutic agent to stop cancer metastases. The process of neoangiogenesis in cervical cancer depends on VEGFR activity. Increased TGFβ1 production is also linked to cervical cancer, suggesting that gene inactivation contributes to the emergence of cervical carcinoma. Group C- was the control group, Group C+ was the cancer model group, CER100 was the group of rats with cancer + 100 mg/kg body weight (BW) of R. tomentosa, CER200 was the group of rats with cancer + 200 mg/kg BW of R. tomentosa, and CER400 was the group of rats with cancer + 400 mg/kg BW R. tomentosa. Rats were dissected after administration of R. tomentosa for 30 days. Immunohistochemical staining of the cervical tissue was performed with TGFβ1 and VEGFR antibodies. VEGFR expression was significantly different from TGFβ1 expression (p < 0.01). The highest expression was observed at the lowest dose of R. tomentosa (100 mg/kg BW), and the lowest expression was observed at 200 and 400 mg/kg BW. The administration of R. tomentosa can repair tissue damage and decrease the expression of TGFβ1 and VEGFR via histopathological parameters, indicating the importance of the activity of these proteins in the development of neoangiogenesis in cervical cancer.

Phyllanthus emblica Linn, a prominent member of the euphorbiaceae family, exhibits extensive distribution across a multitude of tropical and subtropical nations. Referred to as “Balakka” in Indonesia, this plant assumes various names across regions, such as “kimalaka,” “balakka,” “metengo,” “malaka,” and “kemloko” in North Sumatra, Ternate, Sundanese, and Java respectively. Phyllanthus emblica thrives in tropical locales like Indonesia, Malaysia, and Thailand, while also making its presence felt in subtropical regions like India, China, Uzbekistan, and Sri Lanka. The fruits of Balakka are enriched with bioactive constituents recognized for their wide-ranging benefits, including antioxidant, anti-aging, anti-cholesterol, anti-diabetic, immunomodulatory, antipyretic, analgesic, anti-inflammatory, chemoprotective, hepatoprotective, cardioprotective, antimutagenic, and antimicrobial properties. Comprising a spectrum of phenolic compounds (such as tannins, phenolic acids, and flavonoids), alkaloids, phytosterols, terpenoids, organic acids, amino acids, and vitamins, the bioactive components of Malacca fruit offer a diverse array of health-promoting attributes. In light of these insights, this review aims to comprehensively examine the pharmacological activities associated with P. emblica and delve into the intricate composition of its phytochemical constituents.

Context: HIF1α and VEGF are proteins marker oxidative stress and a decrease in placental growth factor (PlGF). Decreasing of HIF1α and VEGF in rats displayed poor trophoblast differentiation, placental abnormalities, and fetal mortality. Rhodomyrtus tomentosa is a flowering plant in the Myrtaceae family that has the potential to be a source of health-promoting chemicals. Aims: To analyze HIF1α and VEGF in serum and hypertension placental tissue after giving Rhodomyrtus tomentosa (RHO) leaves extract. Methods: Six treatments were given to the rats that were identified as being pregnant and pregnant rats with hypertension were given RHO with three doses: (a) normal pregnant rats (control); (b) hypertensive rats; (c) hypertensive rats + 100 mg/kg BW of RHO; (d) hypertensive rats +200 mg/kg BW of RHO; and (e) hypertensive rats + 400 mg/kg BW of RHO and (f) hypertensive rats + nifedipine. Under ketamine anesthesia, pregnant rats were removed on their 20th day of gestation. Immunohistochemistry and ELISA were used to assess HIF1α and VEGF protein expression. Results: There was a significant difference (p0.05) in VEGF expression in the basal zone of the rat placenta across all groups. Conclusions: Rhodomyrtus tomentosa leaves extract decreases HIF1α and VEGF expressions in serum and repairs the tissue of the placenta’s labyrinth, basal, and yolk sacs.

Cervical cancer is one of the most common cancers in Indonesia. It can be treated with molecular therapies targeting Matrix metallopeptidase 9 (MMP-9) and Glucose transporter (GLUT-1), which are enzymes that are involved in tumour cell invasion, metastasis and angiogenesis. Zanthoxylum acanthopodium (andaliman) is an Indonesian herb with anti-cancer properties. This study aimed to investigate the histological changes andaliman treatment caused in MMP-9 and GLUT-1 expression. This study used five groups of rats: control (C-), cancer model (C+), cancer-bearing rats with a 100-mg dose of Zanthoxylum acanthopodium methanol extract (ZAM)/BW (ZAM100), cancer-bearing rats with a 200-mg dose of ZAM /BW (ZAM200) and cancer-bearing rats with a 400-mg dose of ZAM/BW (ZAM400). Immunohistochemical methods were used to stain cervical tissue with MMP-9 and GLUT-1 antibodies, and a TUNEL assay was performed to investigate cell apoptosis. Zanthoxylum acanthopodium methanol extract administration did not affect rat body weight but had a significant effect on cervical cancer growth. There was an increase in MDA levels associated with SOD deficiency in tumour tissue. SOD activity increased due to ZAM administration, allowing cells to be protected from oxidant disruption and oxidative stress. ZAM ameliorated cervical carcinoma tissue damage and reduced the expression of MMP-9, GLUT-1 and apoptosis in serum and tissue (p < 0.01) In short, the higher the ZAM dose, the lower the expression of MMP-9, GLUT-1 and apoptosis, indicating that ZAM is effective to treat cervical cancer.

Phyllanthus emblica (PE) is a plant widely found in Indonesia, especially in Sumatra island, and in India. This study followed the OECD No. 420. The groups were divided based on gender, male and female rats. Male rats were divided into 6 groups, which were male/female control, male/female 2000 mg/kgBW, and male/female 5000 mg/kgBW. This study revealed that the ethanol extract of Phyllanthus emblica (EEPE) doses of 2000 mg/kgBW and 5000 mg/kgBW did not cause histological changes in the heart, liver, testes, ovaries, and kidneys, and did not cause changes to the hematological parameters, kidney biochemical parameters, liver biochemical parameters, and electrolyte parameters both in male and female rats. The results show that the LD50 of EEPE is higher than 5000 mg/kgBW. In short, this study provides information regarding the antioxidant activity and the safe use of EEPE. The LD50 of extract ethanol of Phyllanthus emblica is higher than 5000 mg/kgBW.

Introduction: Rhodomyrtus tomentosa (haramonting), a typical plant of North Sumatera, Indonesia, contains important medicinal ingredients. Nano sized drugs have high loading capacities and can be given at high concentrations. This study aimed to determine the components and toxicity of nanoherbal haramonting. It also aimed to determine the effect of nanoherbal haramonting on the histology of the liver, kidneys, lungs, heart and brain. Methods: High-energy milling was performed to produce nanoherbal haramonting. Thin-layer chromatography was utilised to determine the chemical components of the nanoherb. Antioxidant tests were performed by using the 1,1-diphenyl-2-picryhydrazil method. The three-stage of lethal dose 50 (LD50) which comprised the dose orientation test, preliminary test and actual phase test/LD50 determination, and the Thomson–Weil formula was applied to measure the lethal concentration 50 (LC50) of nanoherbal haramonting. Organs were collected for histological investigation after 14 days of the lethality test. Results: Nanoherbal haramonting had an average diameter distribution of 600.1 nm ± 135.8. It contained flavonoids, steroids, glycosides, saponins and tannins. Its LC50 and LD50 values were 2961.535 ppm and 10.4 ± 0.135 mg/kg BW, respectively. The histology of the heart, kidney, lungs, heart and brain were changed and affected by nanoherbal haramonting treatment at each dose level. Conclusion: Nanoherbal haramonting has strong antioxidative activity and small size, can be effectively used as medicine in the future because it contains secondary metabolite compounds that can be developed as drugs. However, it has mild toxicity.

Background: Pre-eclampsia (PE) contributes to the second cause of maternal death in Indonesia. Andaliman is a typical spice of the Batak ethnic in Northern Sumatera Province, Indonesia. This study aimed to explore the potential of novel herbal medicine compound of nanoherbal andaliman and extra virgin olive oil (EVOO) as PE treatment. Methods: Nanoherbal andaliman was generated using High-energy Milling (HEM). The treatments were divided into the following five groups: K- (control): pregnant rats; K+: PE model rats; P1: PE model rats + 0.45 g of EVOO/200 g BW on the 13th–19th day of pregnancy; P2: PE model rats + nanoherbal andaliman 100 mg/200 g BW on the 13th– 19th day of pregnancy; and P3: PE model rats + combination of 0.45 EVOO/200 g BW and nanoherbal andaliman 100 mg/200 g BW on the 13th–19th day of pregnancy. Rats were dissected on the 20th day of pregnancy. The observed parameters were blood pressure, proteinuria, malondialdehyde (MDA), Heat Shock Protein-70 HSP-70 and histology of placenta. Results: A significant difference was noticed (p0.05). Conclusion: The combination of nanoherbal andaliman and EVOO decreased systolic blood pressure and induced the expression of MDA and HSP-70, as well as placental histology of pre-eclamptic rats.