1. Development of an indirect competitive ELISA based on the common epitope of fish parvalbumin for its detection.
- Author
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Huang Y, Zhu W, Wu Y, Sun L, Li Q, Pramod SN, Wang H, Zhang Z, Lin H, and Li Z
- Subjects
- Animals, Allergens immunology, Allergens analysis, Parvalbumins immunology, Parvalbumins analysis, Enzyme-Linked Immunosorbent Assay methods, Fishes immunology, Epitopes immunology, Fish Proteins immunology, Fish Proteins chemistry
- Abstract
A common epitope (AGSFDHKKFFKACGLSGKST) of parvalbumin from 16 fish species was excavated using bioinformatics tools combined with the characterization of fish parvalbumin binding profile of anti-single epitope antibody in this study. A competitive enzyme-linked immunosorbent assay (ELISA) based on the common epitope was established with a limit of detection of 10.15 ng/mL and a limit of quantification of 49.29 ng/mL. The developed ELISA exhibited a narrow range (71% to 107%) of related cross-reactivity of 15 fish parvalbumin. Besides, the recovery, the coefficient of variations for the intra-assay and the inter-assay were 84.3% to 108.2%, 7.4% to 13.9% and 8.5% to 15.6%. Our findings provide a novel idea for the development of a broad detection method for fish allergens and a practical tool for the detection of parvalbumin of economic fish species in food samples., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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