Search

Your search keyword '"Portillo, Susana"' showing total 31 results
Start Over Author "Portillo, Susana"
31 results on '"Portillo, Susana"'

1. Safety and immunogenicity of a single dose of Tdap compared to Td in pregnant women in Mali and 3 its effect on infant immune responses: a single-centre, randomised, double-blind, active-controlled phase 2 study

Author

Haidara, Fadima Cheick, Tapia, Milagritos D., Diallo, Fatoumata, Portillo, Susana, Williams, Margaret, Traoré, Awa, Rotrosen, Elizabeth, Hensel, Elizabeth, Makowski, Mat, Selamawi, Semhal, Powell, Jonathan A., Kotloff, Karen L., Pasetti, Marcela F., Sow, Samba O., and Neuzil, Kathleen M.

Published
2024
Full Text
View/download PDF

2. Quantitative analysis of pertussis, tetanus, and diphtheria antibodies in sera and breast milk from Tdap vaccinated women using a qualified multiplex assay

Author

Portillo, Susana, primary, Oshinsky, Jennifer, additional, Williams, Margaret, additional, Yoder, Sandra, additional, Liang, Yuanyuan, additional, Campbell, James D., additional, Laufer, Miriam K., additional, Neuzil, Kathleen M., additional, Edwards, Kathryn M., additional, and Pasetti, Marcela F., additional

Published
2024
Full Text
View/download PDF

5. Probing for Trypanosoma cruzi Cell Surface Glycobiomarkers for the Diagnosis and Follow-Up of Chemotherapy of Chagas Disease

Author

Schocker, Nathaniel S., Portillo, Susana, Ashmus, Roger A., Brito, Carlos R. N., Silva, Igor E., Mendoza, Yanira Cordero, Marques, Alexandre F., Monroy, Erika Y., Pardo, Andrew, Izquierdo, Luis, Gállego, Montserrat, Gascon, Joaquim, Almeida, Igor C., Michael, Katja, Witczak, Zbigniew J., editor, and Bielski, Roman, editor

Published
2018
Full Text
View/download PDF

7. Synthetic glycovaccines elicit cross-protection against Chagas disease and tegumentary leishmaniasis in a murine co-infection model

Author

Knight, Colin D, primary, Estevao, Igor L, additional, Portillo, Susana, additional, Farani, Priscilla Silva Grijo, additional, Montoya, Alba, additional, Heydemann, Emily, additional, Carvajal, Elisa G, additional, Vinales, Irodiel, additional, Gil, Eileni R, additional, Rodriguez, Felipe, additional, Maldonado, Rosa A, additional, Michael, Katja, additional, and Almeida, Igor C, additional

Published
2023
Full Text
View/download PDF

8. Purification of Glycosylphosphatidylinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of α-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease

Author

Ortega-Rodriguez, Uriel, primary, Portillo, Susana, additional, Ashmus, Roger A., additional, Duran, Jerry A., additional, Schocker, Nathaniel S., additional, Iniguez, Eva, additional, Montoya, Alba L., additional, Zepeda, Brenda G., additional, Olivas, Janet J., additional, Karimi, Nasim H., additional, Alonso-Padilla, Julio, additional, Izquierdo, Luis, additional, Pinazo, Maria-Jesús, additional, de Noya, Belkisyolé Alarcón, additional, Noya, Oscar, additional, Maldonado, Rosa A., additional, Torrico, Faustino, additional, Gascon, Joaquim, additional, Michael, Katja, additional, and Almeida, Igor C., additional

Published
2019
Full Text
View/download PDF

9. The Trypanosoma brucei MISP family of invariant proteins is co-expressed with BARP as triple helical bundle structures on the surface of salivary gland forms, but is dispensable for parasite development within the tsetse vector

Author

Casas-Sanchez, Aitor, primary, Ramaswamy, Raghavendran, additional, Perally, Samïrah, additional, Haines, Lee R., additional, Rose, Clair, additional, Aguilera-Flores, Marcela, additional, Portillo, Susana, additional, Verbeelen, Margot, additional, Hussain, Shahid, additional, Smithson, Laura, additional, Yunta, Cristina, additional, Lehane, Michael J., additional, Vaughan, Sue, additional, van den Abbeele, Jan, additional, Almeida, Igor C., additional, Boulanger, Martin J., additional, and Acosta-Serrano, Álvaro, additional

Published
2023
Full Text
View/download PDF

12. Probing for Trypanosoma cruzi Cell Surface Glycobiomarkers for the Diagnosis and Follow-Up of Chemotherapy of Chagas Disease

Author

Schocker, Nathaniel S., primary, Portillo, Susana, additional, Ashmus, Roger A., additional, Brito, Carlos R. N., additional, Silva, Igor E., additional, Mendoza, Yanira Cordero, additional, Marques, Alexandre F., additional, Monroy, Erika Y., additional, Pardo, Andrew, additional, Izquierdo, Luis, additional, Gállego, Montserrat, additional, Gascon, Joaquim, additional, Almeida, Igor C., additional, and Michael, Katja, additional

Published
2017
Full Text
View/download PDF

14. Serodiagnosis and therapeutic monitoring of New-World tegumentary leishmaniasis using synthetic type-2 glycoinositolphospholipid-based neoglycoproteins

Author

Viana, Sayonara M., primary, Montoya, Alba L., additional, Carvalho, Augusto M., additional, de Mendonça, Brunele S., additional, Portillo, Susana, additional, Olivas, Janet J., additional, Karimi, Nasim H., additional, Estevao, Igor L., additional, Ortega-Rodriguez, Uriel, additional, Carvalho, Edgar M., additional, Dutra, Walderez O., additional, Maldonaldo, Rosa A., additional, Michael, Katja, additional, de Oliveira, Camila I., additional, and Almeida, Igor C., additional

Published
2022
Full Text
View/download PDF

15. A Branched and Double Alpha-Gal-Bearing Synthetic Neoglycoprotein as a Biomarker for Chagas Disease

Author

Montoya, Alba L., primary, Carvajal, Elisa G., additional, Ortega-Rodriguez, Uriel, additional, Estevao, Igor L., additional, Ashmus, Roger A., additional, Jankuru, Sohan R., additional, Portillo, Susana, additional, Ellis, Cameron C., additional, Knight, Colin D., additional, Alonso-Padilla, Julio, additional, Izquierdo, Luis, additional, Pinazo, Maria-Jesus, additional, Gascon, Joaquim, additional, Suarez, Veronica, additional, Watts, Douglas M., additional, Malo, Iliana R., additional, Ramsey, Janine M., additional, Alarcón De Noya, Belkisyolé, additional, Noya, Oscar, additional, Almeida, Igor C., additional, and Michael, Katja, additional

Published
2022
Full Text
View/download PDF

16. La noche de locas: proceso ritual y orden de género en un espacio masculinizado

Author

Gutiérrez-Portillo, Susana and Gutiérrez-Portillo, Susana

Abstract

In this article I examine a practice and ritual process of university student life called “la noche de locas”. In this ritual process, engineering students at the Autonomous University of Baja California (UABC) prepare a choreography or catwalk “dressed as women.” From a cultural and gender history perspective I seek to understand why the representation of the “locas” emerged in a historically masculinized space, as well as its implications in the production and reproduction of the transgression of gender order within this engineering school. I frame my analysis in an engineering culture in which traditionally masculine meanings predominate as a staple of the discipline, and from which feminine attributes are traditionally excluded. I worked with newspapers and thematic oral history of graduates who participated in (and testified to) this ritual process in different moments. This allowed me to identify several representations of the “ locas” that show small breaks in traditional masculinity models, and that the production of the locas is a space of possibility for creativity, subjectivity and agency of invisible actors. However, since it is a ritual process that occurs during a liminal moment, it fulfills the characteristics of role reversals that end up reproducing and confirming the established gender order., En este artículo analizo una práctica y proceso ritual de la vida estudiantil universitaria denominado “la noche de locas”. En ésta, los estudiantes de ingeniería de la Universidad Autónoma de Baja California (UABC) montan una coreografía o una pasarela “vestidos de mujeres”. Desde la historia cultural y de género busco entender por qué emergió la representación de las locas en un espacio históricamente masculinizado; y qué implicaciones tuvo–y todavía tiene– en producir, reproducir o transgredir el orden de género en la escuela. Enmarco mi análisis en una cultura ingenieril en la que predominan los significados tradicionalmente masculinos como los propios de la disciplina y en la que se excluye lo tradicionalmente femenino. Las fuentes hemerográficas y la historia oral temática de egresados que participaron en (y fueron testigos de) este ritual en diferentes momentos me permitieron identificar representaciones de las locas que evidencian: pequeños quiebres en los esquemas de la masculinidad tradicional, y que la producción de “las locas” es un espacio de posibilidad para la creatividad, subjetividad y agencia de actores invisibilizados. Sin embargo, por tratarse de un ritual que se da en un momento liminal, cumple con las características de inversión de roles que termina reproduciendo y confirmando el orden de género establecido.

Published
2022

18. Reversed Immunoglycomics Identifies α-Galactosyl-Bearing Glycotopes Specific forLeishmania majorInfection

Author

Montoya, Alba L., primary, Austin, Victoria M., additional, Portillo, Susana, additional, Vinales, Irodiel, additional, Ashmus, Roger A., additional, Estevao, Igor, additional, Jankuru, Sohan R., additional, Alraey, Yasser, additional, Al-Salem, Waleed S., additional, Acosta-Serrano, Álvaro, additional, Almeida, Igor C., additional, and Michael, Katja, additional

Published
2021
Full Text
View/download PDF

21. Reversed Immunoglycomics Identifies α-Galactosyl-Bearing Glycotopes Specific for Leishmania majorInfection

Author

Montoya, Alba L., Austin, Victoria M., Portillo, Susana, Vinales, Irodiel, Ashmus, Roger A., Estevao, Igor, Jankuru, Sohan R., Alraey, Yasser, Al-Salem, Waleed S., Acosta-Serrano, Álvaro, Almeida, Igor C., and Michael, Katja

Abstract

All healthy humans have high levels of natural anti-α-galactosyl (α-Gal) antibodies (elicited by yet uncharacterized glycotopes), which may play important roles in immunoglycomics: (a) potential protection against certain parasitic and viral zoonotic infections; (b) targeting of α-Gal-engineered cancer cells; (c) aiding in tissue repair; and (d) serving as adjuvants in α-Gal-based vaccines. Patients with certain protozoan infections have specific anti-α-Gal antibodies, elicited against parasite-derived α-Gal-bearing glycotopes. These glycotopes, however, remain elusive except for the well-characterized glycotope Galα1,3Galβ1,4GlcNAcα, expressed by Trypanosoma cruzi. The discovery of new parasitic glycotopes is greatly hindered by the enormous structural diversity of cell-surface glycans and the technical challenges of classical immunoglycomics, a top-down approach from cultivated parasites to isolated glycans. Here, we demonstrate that reversed immunoglycomics, a bottom-up approach, can identify parasite species-specific α-Gal-bearing glycotopes by probing synthetic oligosaccharides on neoglycoproteins. This method was tested here seeking to identify as-yet unknown glycotopes specific for Leishmania major, the causative agent of Old-World cutaneous leishmaniasis (OWCL). Neoglycoproteins decorated with synthetic α-Gal-containing oligosaccharides derived from L. majorglycoinositolphospholipids served as antigens in a chemiluminescent enzyme-linked immunosorbent assay using sera from OWCL patients and noninfected individuals. Receiver-operating characteristic analysis identified Galpα1,3Galfβ and Galpα1,3Galfβ1,3Manpα glycotopes as diagnostic biomarkers for L. major-caused OWCL, which can distinguish with 100% specificity from heterologous diseases and L. tropica-caused OWCL. These glycotopes could prove useful in the development of rapid α-Gal-based diagnostics and vaccines for OWCL. Furthermore, this method could help unravel cryptic α-Gal-glycotopes of other protozoan parasites and enterobacteria that elicit the natural human anti-α-Gal antibodies.

Published
2021
Full Text
View/download PDF

22. An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major

Author

Iniguez, Eva, primary, Schocker, Nathaniel S., additional, Subramaniam, Krishanthi, additional, Portillo, Susana, additional, Montoya, Alba L., additional, Al-Salem, Waleed S., additional, Torres, Caresse L., additional, Rodriguez, Felipe, additional, Moreira, Otacilio C., additional, Acosta-Serrano, Alvaro, additional, Michael, Katja, additional, Almeida, Igor C., additional, and Maldonado, Rosa A., additional

Published
2017
Full Text
View/download PDF

27. Synthesis of Galα(1,3)Galβ(1,4)GlcNAcα-, Galβ (1,4)GlcNAcα- and GlcNAc-containing neoglycoproteins and their immunological evaluation in the context of Chagas disease.

Author

Schocker, Nathaniel S., Portillo, Susana, Brito, Carlos R. N., Marques, Alexandre F., Almeida, Igor C., and Michael, Katja

Subjects
*CHEMICAL synthesis, *GLYCOSYLPHOSPHATIDYLINOSITOL, *MUCINS, *DIAGNOSIS of Chagas' disease, *CHAGAS' disease, *GLYCOCONJUGATES, *TRYPANOSOMA cruzi, *IMMUNOLOGY, *VACCINATION
Abstract

The protozoan parasite, Trypanosoma cruzi, the etiologic agent of Chagas disease (ChD), has a cell surface covered by immunogenic glycoconjugates. One of the immunodominant glycotopes, the trisaccharide Galα(1,3)Galβ(1,4)GlcNAcα, is expressed on glycosylphosphatidylinositol-anchored mucins of the infective trypomastigote stage of T. cruzi and triggers high levels of protective anti-α-Gal antibodies (Abs) in infected individuals. Here, we have efficiently synthesized the mercaptopropyl glycoside of that glycotope and conjugated it to maleimide-derivatized bovine serum albumin (BSA). Chemiluminescent-enzyme-linked immunosorbent assay revealed that Galα(1,3)Galβ(1,4) GlcNAcα-BSA is recognized by purified anti-α-Gal Abs from chronic ChD patients ~230-fold more strongly than by anti-α-Gal Abs from sera of healthy individuals (NHS anti-α-Gal). Similarly, the pooled sera of chronic Chagas disease patients (ChHSP) recognized Galα(1,3)Galβ(1,4)GlcNAcα ~20-fold more strongly than pooled NHS. In contrast, the underlying disaccharide Galβ(1,4)GlcNAcα and the monosaccharide GlcNAcα or GlcNAcβ conjugated to BSA are poorly or not recognized by purified anti-α-Gal Abs or sera from Chagasic patients or healthy individuals. Our results highlight the importance of the terminal Galα moiety for recognition by Ch anti-α-Gal Abs and the lack of Abs against nonself Galβ(1,4)GlcNAcα and GlcNAcα glycotopes. The substantial difference in binding of Ch vs. NHS anti-α-Gal Abs to Galα(1,3)Galβ(1,4)GlcNAcα-BSA suggests that this neoglycoprotein (NGP) might be suitable for experimental vaccination. To this end, the Galα(1,3)Galβ(1,4) GlcNAcα-BSA NGP was then used to immunize α1,3-galactosyltransferase-knockout mice, which produced antibody titers 40-fold higher as compared with pre-immunization titers. Taken together, our results indicate that the synthetic Galα(1,3)Galβ(1,4)GlcNAcα glycotope coupled to a carrier protein could be a potential diagnostic and vaccine candidate for ChD. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

28. Sinthetic alpha-Gal glycotopes as vacccines and therapeutic biomarkers for Chagas disease and leishmaniasis.

Author

Portillo, Susana, Silva, Igor E., Rodriguez-Ortega, Uriel, Montoya, Alba, Vlana, Sayonara M., Zepeda, Brenda G., Inlguez, Eva A., Ashmus, Roger A., Duran, Jerry A., Schocker, Nathaniel S., Olivas, Janet J., Kariml, Nasim H., Marques, Alexandre F., Alonso-Padllla, Julio, Izquierdo, Luis, Pinazo, Marla-Jesús, Tays Mendes, María, Ellis, Cameron C., Manrlquez Roman, Claudia, and VandeBerg, Jane F.

Published
2020

29. Characterization of antigenic α-Gal-containing extracellular vesicles (EVs) from Trypanosoma cruzi.

Author

Karimi, Nasim Hosseini, Mendes, Maria Tays, Portillo, Susana, Grajeda, Brian, Ellis, Cameron, and Almeida, Igor C.

Subjects
TRYPANOSOMA cruzi, CHAGAS' disease treatment, TRYPANOSOMIASIS, CANCER chemotherapy
Abstract

The article discusses a study characterization of antigenic alfa-Gal-containing extracellular vesicles (EVs) from Trypanosoma cruzi. It reports tha Highly immunogenic alfa-Gal-containing epitopes found on trypomastigote GPI-anchored mucins (tGPI-mucins or TcMUC II) are strongly recognized by patients with Chagas disease (CD). It mentions alfa-Gal glycotopes have been used as BMKs for follow-up of chemotherapy in children and lately in adults with CD, and as prophylactic vaccine targets.

Published
2020

31. Purification of Glycosylphosphatidylinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of α-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease.

Author

Ortega-Rodriguez U, Portillo S, Ashmus RA, Duran JA, Schocker NS, Iniguez E, Montoya AL, Zepeda BG, Olivas JJ, Karimi NH, Alonso-Padilla J, Izquierdo L, Pinazo MJ, de Noya BA, Noya O, Maldonado RA, Torrico F, Gascon J, Michael K, and Almeida IC

Subjects
Animals, Cell Line, Enzyme-Linked Immunosorbent Assay methods, GPI-Linked Proteins chemistry, Glycoproteins chemical synthesis, Humans, Macaca mulatta, Models, Molecular, Mucins chemistry, Protozoan Proteins chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Chagas Disease diagnosis, GPI-Linked Proteins isolation & purification, Glycoproteins chemistry, Mucins isolation & purification, Protozoan Proteins isolation & purification, Trypanosoma cruzi chemistry
Abstract

Chagas disease (ChD), caused by the protozoan parasite Trypanosoma cruzi, affects millions of people worldwide. Chemotherapy is restricted to two drugs, which are partially effective and may cause severe side effects, leading to cessation of treatment in a significant number of patients. Currently, there are no biomarkers to assess therapeutic efficacy of these drugs in the chronic stage. Moreover, no preventive or therapeutic vaccines are available. In this chapter, we describe the purification of Trypanosoma cruzi trypomastigote-derived glycosylphosphatidylinositol (GPI)-anchored mucins (tGPI-mucins) for their use as antigens for the reliable primary or confirmatory diagnosis and as prognostic biomarkers for early assessment of cure following ChD chemotherapy. We also describe, as an example, the synthesis of a potential tGPI-mucin-derived α-Gal-terminating glycan and its coupling to a carrier protein for use as diagnostic and prognostic biomarker in ChD.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results