Search

Your search keyword '"Polakowski RM"' showing total 1 results
Start Over Author "Polakowski RM"

Refine your results

1 results on '"Polakowski RM"'

1. Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection.

Author

Craig DB, Polakowski RM, Arriaga E, Wong JC, Ahmadzadeh H, Stathakis C, and Dovichi NJ

Subjects
Conalbumin analysis, Fluorescein, Fluoresceins, Fluorescence, Fluorescent Dyes, Furans, Lasers, Molecular Weight, Ovalbumin analysis, Polymers, Proteins isolation & purification, Quinolines, Spectrometry, Fluorescence, Trypsinogen analysis, Electrophoresis, Capillary methods, Proteins analysis, Sodium Dodecyl Sulfate
Abstract

We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

Published
1998
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results