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Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection.
- Source :
-
Electrophoresis [Electrophoresis] 1998 Sep; Vol. 19 (12), pp. 2175-8. - Publication Year :
- 1998
-
Abstract
- We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.
- Subjects :
- Conalbumin analysis
Fluorescein
Fluoresceins
Fluorescence
Fluorescent Dyes
Furans
Lasers
Molecular Weight
Ovalbumin analysis
Polymers
Proteins isolation & purification
Quinolines
Spectrometry, Fluorescence
Trypsinogen analysis
Electrophoresis, Capillary methods
Proteins analysis
Sodium Dodecyl Sulfate
Subjects
Details
- Language :
- English
- ISSN :
- 0173-0835
- Volume :
- 19
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Electrophoresis
- Publication Type :
- Academic Journal
- Accession number :
- 9761200
- Full Text :
- https://doi.org/10.1002/elps.1150191222