8 results on '"Pimentel BMS"'
Search Results
2. Merkel cell polyomavirus (MCPyV) DNA prevalence in Brazilian blood donors.
- Author
-
Zucherato VS, da Costa PNM, Giovanetti M, Krause LMF, Alves DCC, Moreira RMA, Pimentel BMS, Haddad R, Bitencourt HT, Ciccozzi M, Alcantara LCJ, Kashima S, Covas DT, and Slavov SN
- Subjects
- Humans, Brazil epidemiology, Prevalence, Blood Donors, DNA, Viral genetics, Merkel cell polyomavirus genetics, Polyomavirus Infections epidemiology, Skin Neoplasms
- Abstract
Merkel cell polyomavirus (MCPyV) is an oncogenic virus that has been etiologically linked to Merkel cell carcinoma. Low levels of MCPyV DNA have been detected in blood donors with unclear impact on transfusion. The prevalence of MCPyV DNA in Brazilian blood donors is unclear. Therefore, the objective of this study was to evaluate the MCPyV DNA prevalence among Brazilian blood donors. We examined the presence of MCPyV DNA by real-time PCR (qPCR) in a total of 450 serum samples obtained from blood donors from three Brazilian regions (North, Central-West and South). The overall estimated MCPyV DNA prevalence was 1.1% (CI = 95%, 0.16-2.06%). Divided by region, in North Brazil (city of Macapa, state of Amapá) and South Brazil (city of Santa Maria, state of Rio Grande do Sul), the MCPyV prevalence was the same: 1.33% (CI = 95%, range 0.0-3.14%). In Central-West Brazil (city of Brasilia), the MCPyV prevalence was 0.6% (CI = 95%, 0.0-1.96%). All MCPyV positive samples showed a high cycle threshold (median Ct = 35.5), most probably related to the low viral load. More studies are necessary to unveil the impact of this oncogenic virus on transfusion medicine and if such exists, especially in regards of its infectivity and transmission potential., (Copyright © 2022 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
3. Molecular identification of the emerging Human Gemykibivirus-2 (HuGkV-2) among Brazilian blood donors.
- Author
-
Zucherato VS, Giovanetti M, Costa LOA, Krause LMF, Alves DCC, Moreira RMA, Pimentel BMS, Haddad R, Bitencourt HT, Ciccozzi M, Alcantara LCJ, Kashima S, Covas DT, and Slavov SN
- Subjects
- Humans, Brazil epidemiology, Prevalence, Blood Donors
- Abstract
Human gemykibivirus-2 (HuGkV-2) belonging to the Gemykibivirus genus (Genomoviridae family) is an emerging DNA virus which has been described as a component of the virome of a wide variety of samples including clinical ones. So far, the HuGkV-2 DNA prevalence in the human population as well as its clinical impact are completely unknown. The objective of this study was to investigate the HuGkV-2 DNA prevalence among Brazilian healthy blood donors from three different geographic regions. A total of 450 blood samples were screened for HuGkV-2 DNA (150 samples were from the Brazilian Amazon, 150 from Midwest Brazil and 150 from South Brazil). The overall HuGkV-2 DNA prevalence was 7.8 %. Considering the examined regions, the highest prevalence was observed in the Brazilian Amazon (city of Macapa, state of Amapa), 15.3 %, followed by the Midwest Brazil (city of Brasilia, Federal District) (6.0 %) and South Brazil (city of Santa Maria, Rio Grande do Sul State) (2.0 %). This study gives preliminary insights on the molecular prevalence of HuGkV-2 DNA among Brazilian blood donors, highlighting that the highest HuGkV-2 prevalence was recorded in the Brazilian Amazon. However, more studies regarding the prevalence, transmission routes and any possible clinical effects appear to be crucial in order to understand the impact of this emerging viral agent., Competing Interests: Declaration of competing interest The authors declare no conflict of interests., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
4. Chikungunya virus seroprevalence in asymptomatic blood donors during an outbreak in the Federal District of Brazil.
- Author
-
da Silva Mendes AM, Cilião-Alves DC, Pimentel BMS, Slavov SN, de Araújo WN, and Haddad R
- Subjects
- Acute Disease, Animals, Antibodies, Viral, Blood Donors, Brazil epidemiology, Disease Outbreaks, Humans, Immunoglobulin G, Immunoglobulin M, Seroepidemiologic Studies, Chikungunya Fever diagnosis, Chikungunya Fever epidemiology, Chikungunya virus genetics
- Abstract
Introduction: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus belonging to the Togaviridae family. The symptomatic infection is characterised by acute febrile disease which generally results in severe arthralgia and myalgia, however, most of the CHIKV infections remain asymptomatic. CHIKV RNA detection in asymptomatic volunteers may be responsible for the transfusion transmission of this infection, especially during outbreaks. There is no information for CHIKV seroprevalence among blood donors from the Federal District of Brazil., Aim: In early 2019, the Federal District of Brazil experienced a CHIKV outbreak, and this study evaluates the anti-CHIKV IgM and IgG presence in a well characterised cohort of blood donors from this region., Methodology: Blood samples were collected from 450 volunteer blood donors during a CHIKV outbreak and tested for the presence of anti-CHIKV IgG and IgM antibodies using ELISA., Results: The CHIKV seroprevalence was 0.89% (n = 4/450) and anti-CHIKV IgM prevalence was 1.11% (n = 5/450)., Conclusion: The obtained results demonstrated that at least some of the blood donors have experienced CHIKV infection which can be related to a hypothetical risk of CHIKV transfusion transmission. More studies are necessary in order to examine the impact of CHIKV on blood transfusion., (© 2022 British Blood Transfusion Society.)
- Published
- 2022
- Full Text
- View/download PDF
5. High seroprevalence of anti-DENV IgM in blood donors in an early outbreak in the Federal District of Brazil, 2019.
- Author
-
Cilião-Alves DC, Barcelos SM, Bezerra ALO, Almeida IS, Silveira JVM, Alencar JP, Pimentel BMS, Araújo WN, and Haddad R
- Subjects
- Adult, Brazil epidemiology, Female, Humans, Male, Middle Aged, Prevalence, Retrospective Studies, Seroepidemiologic Studies, Young Adult, Antibodies, Viral blood, Blood Donors, Dengue epidemiology, Dengue immunology, Disease Outbreaks, Immunoglobulin M blood
- Abstract
Dengue is endemic in Brazil, and several Brazilian cities are affected by frequent seasonal outbreaks of the disease. During the outbreaks the possibility of transfusion-transmitted dengue (TTD) is increased, mainly by the presence of asymptomatic or oligosymptomatic infections in eligible blood donors. The retrospective assessment of anti-DENV IgM and NS1 seroprevalence during a given time interval may indicate the need for measures for the previous screening of DENV infection in blood donors. In this context, we performed retrospective screening for anti-DENV IgM and NS1 in blood donors from the Federal District of Brazil during the early outbreak that occurred in 2019, the largest outbreak in recent years. In total, 450 blood donations were screened for anti-DENV IgM and DENV NS1 using commercial enzyme-linked immunosorbent assay kits (Panbio Dengue IgM Capture ELISA and Platelia Dengue NS1 Ag, respectively). Among the tested plasma samples, 16 % (72/450) presented anti-DENV IgM; no samples presented DENV NS1. Despite the apparent absence of antigenaemia in tested blood donations, the high prevalence of anti-DENV IgM highlights the importance of DENV screening in blood donors, principally during outbreak periods.
- Published
- 2021
- Full Text
- View/download PDF
6. Zika virus RNA surveillance in blood donors in the Federal District of Brazil during the 2016 outbreak.
- Author
-
Slavov SN, Gonzaga FAC, Pimentel BMS, Ramos DDAR, de Araújo WN, Covas DT, Kashima S, and Haddad R
- Published
- 2020
- Full Text
- View/download PDF
7. Low human parvovirus B19 (B19V) DNA prevalence in blood donors from Central-West Brazil.
- Author
-
Slavov SN, Gonçalves de Noronha LA, Gonzaga FAC, Pimentel BMS, Kashima S, and Haddad R
- Subjects
- Adult, Antibodies, Viral blood, Asymptomatic Infections epidemiology, Blood Transfusion, Brazil epidemiology, Female, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Male, Mass Screening, Middle Aged, Parvoviridae Infections transmission, Parvovirus B19, Human, Polymerase Chain Reaction, Prevalence, Serologic Tests, Young Adult, Blood Donors, DNA, Viral blood, Parvoviridae Infections blood, Parvoviridae Infections epidemiology, Viral Load
- Abstract
Parvovirus B19 (B19V) transmission may occur through blood transfusion as a result of asymptomatic viral persistence in blood donors. Our study evaluated the prevalence and viral load of B19V in blood donors from Brasilia, Federal District, Central-West Brazil. B19V DNA detection and quantification were performed in 477 blood donors. The positive samples were also tested for anti-B19V IgG and haemoderivative recipients were investigated for adverse effects following transfusion. B19V DNA prevalence was 0.21 % (n=1/477). The positive B19V DNA sample was also anti-B19 IgG-positive (probably persistent infection). The viral load was low and no adverse effects following blood transfusion were registered in the recipients. This study demonstrated that the B19V DNA prevalence in blood donors from Central-West Brazil is low. Nevertheless, the mere presence of B19V DNA in blood donors strengthens the need for viral molecular screening, especially in haemoderivatives that that will go to susceptible recipients.
- Published
- 2019
- Full Text
- View/download PDF
8. Dengue seroprevalence among asymptomatic blood donors during an epidemic outbreak in Central-West Brazil.
- Author
-
Slavov SN, Cilião-Alves DC, Gonzaga FAC, Moura DR, de Moura ACAM, de Noronha LAG, Cassemiro ÉM, Pimentel BMS, Costa FJQ, da Silva GA, Ramos DDAR, de Araújo WN, Kashima S, and Haddad R
- Subjects
- Adult, Antibodies, Viral immunology, Blood Transfusion, Brazil epidemiology, Cohort Studies, Dengue blood, Dengue epidemiology, Dengue virology, Dengue Virus genetics, Female, Humans, Immunoglobulin G immunology, Immunoglobulin M immunology, Male, Pilot Projects, RNA, Viral genetics, Seroepidemiologic Studies, Viremia blood, Viremia epidemiology, Viremia immunology, Viremia virology, Antibodies, Viral blood, Asymptomatic Infections epidemiology, Blood Donors statistics & numerical data, Dengue immunology, Dengue Virus immunology, Disease Outbreaks, Viral Nonstructural Proteins immunology
- Abstract
Dengue virus (DENV) transmission by blood transfusion is an important route of viral acquisition during outbreaks. The prevalence of DENV markers (viral RNA, NS1, anti-DENV IgM, and IgG) among blood donors in Central-West Brazil has never been evaluated. Our aim was to evaluate the full set of serological and molecular markers for DENV among blood donors of the Federal District of Brazil during an extensive outbreak in 2016. We found an anti-DENV IgM prevalence of 6.74% (n = 32/475). Of 475, 20 samples (4.21%) were also anti-DENV IgG positive. All samples were non-reactive for NS1 and DENV RNA. Our results imply that a significant proportion of the tested donors had experienced asymptomatic infection. More studies are necessary to evaluate the real prevalence of DENV viremia in blood donors from the Federal District of Brazil and if specific measures are needed to routinely test the blood donors for DENV RNA during outbreaks., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.