1. Blue-light filtering alters angiogenic signaling in human retinal pigmented epithelial cells culture model
- Author
-
Natalia Vila, Sultan Aldrees, Evangelina Esposito, Pablo Zoroquiain, Patrick Logan, Aya Siblini, Miguel N. Burnier, Lluis Arias, Vasco Bravo-Filho, and Universitat de Barcelona
- Subjects
0301 basic medicine ,Lutein ,Light ,Angiogenesis ,Retinal Pigment Epithelium ,Epithelial cells ,medicine.disease_cause ,chemistry.chemical_compound ,Macular Degeneration ,0302 clinical medicine ,lcsh:Ophthalmology ,Hypoxia ,Cells, Cultured ,Cèl·lules epitelials ,Neovascularization, Pathologic ,General Medicine ,Anatomy ,Cell biology ,Llum ,Cytokines ,Physiological effects of radiation | Physiological effects of radiation ,medicine.symptom ,Research Article ,Blue light filters ,Signal Transduction ,Cellular signal transduction ,Angiogenin ,Estrès oxidatiu ,Citologia ,RPE cell line ,03 medical and health sciences ,medicine ,Humans ,Secretion ,Efectes fisiològics de les radiacions ,Neovascularization ,Blue light ,business.industry ,Age-related macular degeneration ,Transducció de senyal cel·lular ,Retinal ,Epithelial Cells ,Hypoxia (medical) ,Angiogènesi ,Physiological effects of radiation Physiological effects of radiation ,Ophthalmology ,Oxidative Stress ,030104 developmental biology ,chemistry ,lcsh:RE1-994 ,Oxidative stress ,030221 ophthalmology & optometry ,business ,Cytology - Abstract
Background Light exposure and more specifically the spectrum of blue light contribute to the oxidative stress in Age-related macular degeneration (AMD). The purpose of the study was to establish whether blue light filtering could modify proangiogenic signaling produced by retinal pigmented epithelial (RPE) cells under different conditions simulating risk factors for AMD. Methods Three experiments were carried out in order to expose ARPE-19 cells to white light for 48 h with and without blue light-blocking filters (BLF) in different conditions. In each experiment one group was exposed to light with no BLF protection, a second group was exposed to light with BLF protection, and a control group was not exposed to light. The ARPE-19 cells used in each experiment prior to light exposure were cultured for 24 h as follows: Experiment 1) Normoxia, Experiment 2) Hypoxia, and Experiment 3) Lutein supplemented media in normoxia. The media of all groups was harvested after light exposure for sandwich ELISA-based assays to quantify 10 pro-angiogenic cytokines. Results A significant decrease in angiogenin secretion levels and a significant increase in bFGF were observed following light exposure, compared to dark conditions, in both normoxia and hypoxia conditions. With the addition of a blue light-blocking filter in normoxia, a significant increase in angiogenin levels was observed. Although statistical significance was not achieved, blue light filters reduce light-induced secretion of bFGF and VEGF to near normal levels. This trend is also observed when ARPE-19 cells are grown under hypoxic conditions and when pre-treated with lutein prior to exposure to experimental conditions. Conclusions Following light exposure, there is a decrease in angiogenin secretion by ARPE-19 cells, which was abrogated with a blue light - blocking filter. Our findings support the position that blue light filtering affects the secretion of angiogenic factors by retinal pigmented epithelial cells under normoxic, hypoxic, and lutein-pretreated conditions in a similar manner.
- Published
- 2017