Back to Search
Start Over
Blue-light filtering alters angiogenic signaling in human retinal pigmented epithelial cells culture model
- Source :
- Dipòsit Digital de la UB, Universidad de Barcelona, BMC Ophthalmology, Recercat. Dipósit de la Recerca de Catalunya, instname, BMC Ophthalmology, Vol 17, Iss 1, Pp 1-7 (2017)
- Publication Year :
- 2017
- Publisher :
- BioMed Central, 2017.
-
Abstract
- Background Light exposure and more specifically the spectrum of blue light contribute to the oxidative stress in Age-related macular degeneration (AMD). The purpose of the study was to establish whether blue light filtering could modify proangiogenic signaling produced by retinal pigmented epithelial (RPE) cells under different conditions simulating risk factors for AMD. Methods Three experiments were carried out in order to expose ARPE-19 cells to white light for 48 h with and without blue light-blocking filters (BLF) in different conditions. In each experiment one group was exposed to light with no BLF protection, a second group was exposed to light with BLF protection, and a control group was not exposed to light. The ARPE-19 cells used in each experiment prior to light exposure were cultured for 24 h as follows: Experiment 1) Normoxia, Experiment 2) Hypoxia, and Experiment 3) Lutein supplemented media in normoxia. The media of all groups was harvested after light exposure for sandwich ELISA-based assays to quantify 10 pro-angiogenic cytokines. Results A significant decrease in angiogenin secretion levels and a significant increase in bFGF were observed following light exposure, compared to dark conditions, in both normoxia and hypoxia conditions. With the addition of a blue light-blocking filter in normoxia, a significant increase in angiogenin levels was observed. Although statistical significance was not achieved, blue light filters reduce light-induced secretion of bFGF and VEGF to near normal levels. This trend is also observed when ARPE-19 cells are grown under hypoxic conditions and when pre-treated with lutein prior to exposure to experimental conditions. Conclusions Following light exposure, there is a decrease in angiogenin secretion by ARPE-19 cells, which was abrogated with a blue light - blocking filter. Our findings support the position that blue light filtering affects the secretion of angiogenic factors by retinal pigmented epithelial cells under normoxic, hypoxic, and lutein-pretreated conditions in a similar manner.
- Subjects :
- 0301 basic medicine
Lutein
Light
Angiogenesis
Retinal Pigment Epithelium
Epithelial cells
medicine.disease_cause
chemistry.chemical_compound
Macular Degeneration
0302 clinical medicine
lcsh:Ophthalmology
Hypoxia
Cells, Cultured
Cèl·lules epitelials
Neovascularization, Pathologic
General Medicine
Anatomy
Cell biology
Llum
Cytokines
Physiological effects of radiation | Physiological effects of radiation
medicine.symptom
Research Article
Blue light filters
Signal Transduction
Cellular signal transduction
Angiogenin
Estrès oxidatiu
Citologia
RPE cell line
03 medical and health sciences
medicine
Humans
Secretion
Efectes fisiològics de les radiacions
Neovascularization
Blue light
business.industry
Age-related macular degeneration
Transducció de senyal cel·lular
Retinal
Epithelial Cells
Hypoxia (medical)
Angiogènesi
Physiological effects of radiation Physiological effects of radiation
Ophthalmology
Oxidative Stress
030104 developmental biology
chemistry
lcsh:RE1-994
Oxidative stress
030221 ophthalmology & optometry
business
Cytology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Dipòsit Digital de la UB, Universidad de Barcelona, BMC Ophthalmology, Recercat. Dipósit de la Recerca de Catalunya, instname, BMC Ophthalmology, Vol 17, Iss 1, Pp 1-7 (2017)
- Accession number :
- edsair.doi.dedup.....27b303fed59eaf408502cb9e392fe99d