10 results on '"Phullara B. Shelat"'
Search Results
2. Phospholipases A2 and Inflammatory Responses in the Central Nervous System
- Author
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Michael D. Jensen, Grace Y. Sun, Phullara B. Shelat, Albert Y. Sun, Agnes Simonyi, and Yan He
- Subjects
Central Nervous System ,Central nervous system ,Apoptosis ,Inflammation ,Phospholipase ,Biology ,Receptors, N-Methyl-D-Aspartate ,p38 Mitogen-Activated Protein Kinases ,Article ,Brain Ischemia ,Cellular and Molecular Neuroscience ,Cytosol ,Alzheimer Disease ,Memory ,Catalytic Domain ,medicine ,Phospholipid homeostasis ,Humans ,RNA, Messenger ,Phosphorylation ,Receptor ,Phospholipids ,Neurons ,NADPH Oxidases ,Neurodegenerative Diseases ,Lipid signaling ,Cell biology ,Phospholipases A2 ,medicine.anatomical_structure ,Neurology ,Biochemistry ,Molecular Medicine ,Neuroglia ,lipids (amino acids, peptides, and proteins) ,medicine.symptom - Abstract
Phospholipases A2 (PLA2s) belong to a superfamily of enzymes responsible for hydrolyzing the sn-2 fatty acids of membrane phospholipids. These enzymes are known to play multiple roles for maintenance of membrane phospholipid homeostasis and for production of a variety of lipid mediators. Over 20 different types of PLA2s are present in the mammalian cells, and in snake and bee venom. Despite their common function in hydrolyzing fatty acids of phospholipids, they are diversely encoded by a number of genes and express proteins that are regulated by different mechanisms. Recent studies have focused on the group IV calcium-dependent cytosolic cPLA2, the group VI calcium-independent iPLA2, and the group II small molecule secretory sPLA2. In the central nervous system (CNS), these PLA2s are distributed among neurons and glial cells. Although the physiological role of these PLA2s in regulating neural cell function has not yet been clearly elucidated, there is increasing evidence for their involvement in receptor signaling and transcriptional pathways that link oxidative events to inflammatory responses that underline many neurodegenerative diseases. Recent studies also reveal an important role of cPLA2 in modulating neuronal excitatory functions, sPLA2 in the inflammatory responses, and iPLA2 with childhood neurologic disorders associated with brain iron accumulation. The goal for this review is to better understand the structure and function of these PLA2s and to highlight specific types of PLA2s and their cross-talk mechanisms in these inflammatory responses under physiological and pathological conditions in the CNS.
- Published
- 2009
3. Amyloid beta peptide and NMDA induce ROS from NADPH oxidase and AA release from cytosolic phospholipase A2in cortical neurons
- Author
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Albert Y. Sun, Joanna B. Strosznajder, Grace Y. Sun, Jing-Hung Wang, Phullara B. Shelat, Malgorzata Chalimoniuk, Agnes Simonyi, and James C. Lee
- Subjects
N-Methylaspartate ,Amyloid beta ,Phospholipases A2, Cytosolic ,Receptors, N-Methyl-D-Aspartate ,Biochemistry ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Phospholipase A2 ,Alzheimer Disease ,Excitatory Amino Acid Agonists ,medicine ,Animals ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Cells, Cultured ,Cerebral Cortex ,Neurons ,Phospholipase A ,Amyloid beta-Peptides ,Arachidonic Acid ,NADPH oxidase ,biology ,Glutamate receptor ,Memantine ,NADPH Oxidases ,Peptide Fragments ,Rats ,Oxidative Stress ,Synaptic plasticity ,biology.protein ,NMDA receptor ,Reactive Oxygen Species ,Excitatory Amino Acid Antagonists ,Signal Transduction ,medicine.drug - Abstract
Increase in oxidative stress has been postulated to play an important role in the pathogenesis of a number of neurodegenerative diseases including Alzheimer's disease. There is evidence for involvement of amyloid-beta peptide (Abeta) in mediating the oxidative damage to neurons. Despite yet unknown mechanism, Abeta appears to exert action on the ionotropic glutamate receptors, especially the N-methyl-D-aspartic acid (NMDA) receptor subtypes. In this study, we showed that NMDA and oligomeric Abeta(1-42) could induce reactive oxygen species (ROS) production from cortical neurons through activation of NADPH oxidase. ROS derived from NADPH oxidase led to activation of extracellular signal-regulated kinase 1/2, phosphorylation of cytosolic phospholipase A(2)alpha (cPLA(2)alpha), and arachidonic acid (AA) release. In addition, Abeta(1-42)-induced AA release was inhibited by d(-)-2-amino-5-phosphonopentanoic acid and memantine, two different NMDA receptor antagonists, suggesting action of Abeta through the NMDA receptor. Besides serving as a precursor for eicosanoids, AA is also regarded as a retrograde messenger and plays a role in modulating synaptic plasticity. Other phospholipase A(2) products such as lysophospholipids can perturb membrane phospholipids. These results suggest an oxidative-degradative mechanism for oligomeric Abeta(1-42) to induce ROS production and stimulate AA release through the NMDA receptors. This novel mechanism may contribute to the oxidative stress hypothesis and synaptic failure that underline the pathogenesis of Alzheimer's disease.
- Published
- 2008
4. Phospholipases A2Mediate Amyloid-β Peptide-Induced Mitochondrial Dysfunction
- Author
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Yinzhi Lai, Phullara B. Shelat, Chunhua Hu, James C. Lee, Grace Y. Sun, and Donghui Zhu
- Subjects
Amyloid beta ,Mitochondrion ,medicine.disease_cause ,Phospholipases A ,Cytosol ,medicine ,Animals ,Protein kinase A ,Cells, Cultured ,Amyloid beta-Peptides ,NADPH oxidase ,biology ,General Neuroscience ,Articles ,Peptide Fragments ,Mitochondria ,Rats ,Cell biology ,Phospholipases A2 ,Biochemistry ,Astrocytes ,biology.protein ,Phosphorylation ,lipids (amino acids, peptides, and proteins) ,Calcium ,Signal transduction ,Reactive Oxygen Species ,Oxidative stress - Abstract
Mitochondrial dysfunction has been implicated in the pathophysiology of Alzheimer's disease (AD) brains. To unravel the mechanism(s) underlying this dysfunction, we demonstrate that phospholipases A2(PLA2s), namely the cytosolic and the calcium-independent PLA2s (cPLA2and iPLA2), are key enzymes mediating oligomeric amyloid-β peptide (Aβ1–42)-induced loss of mitochondrial membrane potential and increase in production of reactive oxygen species from mitochondria in astrocytes. Whereas the action of iPLA2is immediate, the action of cPLA2requires a lag time of ∼12–15 min, probably the time needed for initiating signaling pathways for the phosphorylation and translocation of cPLA2to mitochondria. Western blot analysis indicated the ability of oligomeric Aβ1–42to increase phosphorylation of cPLA2in astrocytes through the NADPH oxidase and mitogen-activated protein kinase pathways. The involvement of PLA2in Aβ1–42-mediated perturbations of mitochondrial function provides new insights to the decline in mitochondrial function, leading to impairment in ATP production and increase in oxidative stress in AD brains.
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- 2006
5. Polyphenols in Cerebral Ischemia: Novel Targets for Neuroprotection
- Author
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Rebecca L. Miller, Phullara B. Shelat, Albert Y. Sun, Grace Y. Sun, Mozow Yusof, Agnes Simonyi, and Qun Wang
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Necrosis ,Antioxidant ,medicine.medical_treatment ,Neuroscience (miscellaneous) ,Ischemia ,Oxidative phosphorylation ,Biology ,medicine.disease_cause ,Neuroprotection ,Brain Ischemia ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Drug Delivery Systems ,Phenols ,medicine ,Animals ,Humans ,Flavonoids ,chemistry.chemical_classification ,Reactive oxygen species ,Plant Extracts ,Polyphenols ,food and beverages ,medicine.disease ,Oxidative Stress ,Neuroprotective Agents ,Neurology ,Biochemistry ,chemistry ,Curcumin ,medicine.symptom ,Oxidative stress - Abstract
Plant polyphenols are dietary components that exert a variety of biochemical and pharmacological effects. Recently, considerable interest has been focused on polyphenols because of their antioxidant, anti-inflammatory, and antiproliferative activities. Oxidative stress is thought to be a key event in the pathogenesis of cerebral ischemia. Overproduction of reactive oxygen species during ischemia/reperfusion could cause an imbalance between oxidative and antioxidative processes. Reactive oxygen species can damage lipids, proteins, and nucleic acids, thereby inducing apoptosis or necrosis. There is increasing evidence supporting the hypothesis that plant polyphenols can provide protection against neurodegenerative changes associated with cerebral ischemia. This article reviews the neuroprotective effects of plant extracts and their constituents that have been used in animal models of cerebral ischemia. The use of polyphenols as therapeutic agents in stroke has been suggested.
- Published
- 2005
6. P4‐172: Abeta activates cytosolic phospholipase A2 through an NMDA signaling pathway involving NADPH oxidase in cortical neurons
- Author
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Phullara B. Shelat, Grace Y. Sun, Agnes Simonyi, James C. Lee, and Albert Y. Sun
- Subjects
NADPH oxidase ,biology ,Epidemiology ,Chemistry ,Health Policy ,Cortical neurons ,Cell biology ,Psychiatry and Mental health ,Cellular and Molecular Neuroscience ,Cytosol ,Phospholipase A2 ,Developmental Neuroscience ,biology.protein ,NMDA receptor ,Neurology (clinical) ,Geriatrics and Gerontology ,Signal transduction - Published
- 2008
7. Differential roles of hippocampal metabotropic glutamate receptors 1 and 5 in inhibitory avoidance learning
- Author
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Marsha M. Dopheide, Aminata P. Coulibaly, Peter Serfozo, Phullara B. Shelat, Todd R. Schachtman, and Agnes Simonyi
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Male ,medicine.drug_class ,Pyridines ,Cognitive Neuroscience ,Receptor, Metabotropic Glutamate 5 ,Glycine ,Hippocampus ,Experimental and Cognitive Psychology ,Hippocampal formation ,Receptors, Metabotropic Glutamate ,Benzoates ,Article ,Extinction, Psychological ,Rats, Sprague-Dawley ,Behavioral Neuroscience ,medicine ,Avoidance Learning ,Animals ,Glutamate receptor ,Antagonist ,Retention, Psychology ,Neural Inhibition ,Receptor antagonist ,Rats ,Metabotropic receptor ,Metabotropic glutamate receptor ,Synaptic plasticity ,Psychology ,Neuroscience ,Excitatory Amino Acid Antagonists - Abstract
Group I metabotropic glutamate receptors (mGlu1 and 5) have been implicated in synaptic plasticity and learning and memory. However, much of our understanding of how these receptors in different brain regions contribute to distinct memory stages in different learning tasks remains incomplete. The present study investigated the effects of the mGlu5 receptor antagonist, 2-methyl-6-(phenylethynyl)-pyridine (MPEP), and mGlu1 receptor antagonist, ( S )-(+)-α-amino-4-carboxy-2-methylbenzene-acetic acid (LY 367385) in the dorsal hippocampus on the consolidation and extinction of memory for inhibitory avoidance learning. Male, Sprague–Dawley rats were trained in a single-trial step-down inhibitory avoidance task. MPEP, LY 367385 or saline were infused bilaterally into the CA1 region immediately after training or immediately after the first retention test which was given 24 h after training. Rats receiving MPEP (1.5 or 5.0 μg/side) or LY 367385 (0.7 or 2.0 μg/side) infusion exhibited a dose-dependent decrease in retention when tested 24 h later. MPEP was ineffective while LY 367385 significantly attenuated extinction when injected after the first retention test using an extinction procedure. These findings indicate a selective participation of hippocampal group I mGlu receptors in memory processing in this task.
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- 2007
8. Ischemia-induced increase in RGS7 mRNA expression in gerbil hippocampus
- Author
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Grace Y. Sun, Qun Wang, Phullara B. Shelat, Agnes Simonyi, Aminata P. Coulibaly, and Albert Y. Sun
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Male ,medicine.medical_specialty ,General Neuroscience ,Dentate gyrus ,Ischemia ,Hippocampus ,In situ hybridization ,Biology ,medicine.disease ,Gerbil ,Brain Ischemia ,Endocrinology ,nervous system ,Internal medicine ,Gene expression ,medicine ,Animals ,sense organs ,RNA, Messenger ,Receptor ,Gerbillinae ,RGS2 ,RGS Proteins - Abstract
The present study investigated the changes in the expression of regulators of G-protein-coupled signaling proteins RGS2, 7 and 8 in gerbil hippocampus to better understand alterations of G-protein-coupled receptors signaling after cerebral ischemia. In situ hybridization revealed a transient, robust early increase in RGS7 mRNA levels in the dentate gyrus after ischemia. RGS8 mRNA expression started to increase at a later time point in the CA3 region but no changes were found for RGS2. Our results show a subtype-, time-, and subregion-specific regulation in mRNA expression of RGS proteins after cerebral ischemia in gerbil hippocampus.
- Published
- 2006
9. Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits
- Author
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Albert Y. Sun, Phullara B. Shelat, Dennis K. Miller, Michael D. Jensen, Qun Wang, Ruth S. MacDonald, Gary A. Weisman, Grace Y. Sun, Dennis E. Lubahn, Agnes Simonyi, and George E. Rottinghaus
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Male ,Curcumin ,Indoles ,Time Factors ,Ischemia ,Apoptosis ,Cell Count ,Behavioral Symptoms ,Pharmacology ,Mitochondrion ,Biology ,CD13 Antigens ,Motor Activity ,medicine.disease_cause ,Neuroprotection ,Brain Ischemia ,Lipid peroxidation ,Brain ischemia ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Lectins ,Glial Fibrillary Acidic Protein ,medicine ,In Situ Nick-End Labeling ,Animals ,Neurons ,Analysis of Variance ,Behavior, Animal ,Caspase 3 ,Histocytochemistry ,Brain ,Cytochromes c ,medicine.disease ,Immunohistochemistry ,Mitochondria ,Disease Models, Animal ,Neuroprotective Agents ,chemistry ,Liver ,Astrocytes ,Caspases ,Immunology ,Lipid Peroxidation ,Microglia ,Gerbillinae ,Oxidative stress - Abstract
Increased oxidative stress has been regarded as an important underlying cause for neuronal damage induced by cerebral ischemia/reperfusion (I/R) injury. In recent years, there has been increasing interest in investigating polyphenols from botanical source for possible neuroprotective effects against neurodegenerative diseases. In this study, we investigated the mechanisms underlying the neuroprotective effects of curcumin, a potent polyphenol antioxidant enriched in tumeric. Global cerebral ischemia was induced in Mongolian gerbils by transient occlusion of the common carotid arteries. Histochemical analysis indicated extensive neuronal death together with increased reactive astrocytes and microglial cells in the hippocampal CA1 area at 4 days after I/R. These ischemic changes were preceded by a rapid increase in lipid peroxidation and followed by decrease in mitochondrial membrane potential, increased cytochrome c release, and subsequently caspase-3 activation and apoptosis. Administration of curcumin by i.p. injections (30 mg/kg body wt) or by supplementation to the AIN76 diet (2.0 g/kg diet) for 2 months significantly attenuated ischemia-induced neuronal death as well as glial activation. Curcumin administration also decreased lipid peroxidation, mitochondrial dysfunction, and the apoptotic indices. The biochemical changes resulting from curcumin also correlated well with its ability to ameliorate the changes in locomotor activity induced by I/R. Bioavailability study indicated a rapid increase in curcumin in plasma and brain within 1 hr after treatment. Together, these findings attribute the neuroprotective effect of curcumin against I/R-induced neuronal damage to its antioxidant capacity in reducing oxidative stress and the signaling cascade leading to apoptotic cell death.
- Published
- 2005
10. Neuroprotective mechanisms of curcumin against cerebral ischemia‐induced neuronal apoptosis and behavioral deficits.
- Author
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Qun Wang, Albert Y. Sun, Agnes Simonyi, Michael D. Jensen, Phullara B. Shelat, George E. Rottinghaus, Ruth S. MacDonald, Dennis K. Miller, Dennis E. Lubahn, Gary A. Weisman, and Grace Y. Sun
- Published
- 2005
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