204 results on '"Petkevičius, Saulius"'
Search Results
2. Reducing anthelmintic inputs in organic farming: Are small ruminant farmers integrating alternative strategies to control gastrointestinal nematodes?
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Chylinski, Caroline, Athanasiadou, Spiridoula, Thüer, Susann, Grovermann, Christian, Moakes, Simon, Hoste, Hervé, Petkevicius, Saulius, Verwer, Cynthia, Verkaik, Jan, and Werne, Steffen
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- 2023
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3. First Molecular Characterization of Small Ruminant Lentiviruses in Hungarian Goat Population.
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Ózsvári, László, Bárdos, Krisztina, Moroz-Fik, Agata, Biernacka, Kinga, Mickiewicz, Marcin, Nowek, Zofia, Abril, Carlos Eduardo, Bertoni, Giuseppe, Stuen, Snorre, Petkevičius, Saulius, Kaba, Jarosław, and Czopowicz, Michał
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ANIMAL herds ,DNA sequencing ,GOATS ,GENOTYPES ,LENTIVIRUSES - Abstract
In 2023, a molecular study was conducted on the Hungarian goat population to determine genotypes and subtypes of small ruminant lentiviruses (SRLV) infecting these herds. Ten goat herds seropositive for SRLV infection according to a serosurvey conducted earlier in Hungary were selected, and 135 adult goats (>1 year old) were blood sampled. The two-stage nested real-time PCR (nRT-PCR) was used to detect proviral DNA of SRLV and distinguish between two main viral genotypes (A and B). PCR products were submitted for Sanger dideoxy sequencing, and phylogenetic and molecular evolutionary analyses were conducted on the 200–250 bp-long proviral DNA sequences from the end of long terminal repeat (LTR) region and beginning of gag gene using the MEGA11 software. Reference strains included strains most identical to Hungarian sequences according to the Standard Nucleotide BLAST and prototypic strains for the relevant genotypes and subtypes. Proviral DNA of SRLV was detected in goats from all ten tested herds. A single SRLV genotype was detected in 6 herds—genotype A in three herds and B also in three herds. In four herds, mixed infection with genotypes A and B was confirmed. In total, 110/135 seropositive goats tested positive in the nRT-PCR (81.5%): 49/110 goats (44.5%) for genotype A, 54/110 goats (49.1%) for genotype B, and 7/110 goats (6.4%) for both genotypes. Hungarian sequences belonged to subtypes A1/A18, A2, and subtype B1. This is the first study which shows that Hungarian goats are infected by SRLV belonging to both genotypes A and B. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Anthelmintic resistance of horse strongyle nematodes to fenbendazole in Lithuania
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Dauparaitė, Evelina, Kupčinskas, Tomas, Varady, Marian, and Petkevičius, Saulius
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- 2022
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5. Anthelmintic resistance in small ruminants in the Nordic-Baltic region
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Beleckė, Agnė, Kupčinskas, Tomas, Stadalienė, Inga, Höglund, Johan, Thamsborg, Stig Milan, Stuen, Snorre, and Petkevičius, Saulius
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- 2021
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6. Anthelmintic resistance of horse strongyle nematodes to ivermectin and pyrantel in Lithuania
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Dauparaitė, Evelina, Kupčinskas, Tomas, von Samson-Himmelstjerna, Georg, and Petkevičius, Saulius
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- 2021
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7. Parasite control in organic cattle farming: Management and farmers' perspectives from six European countries
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Takeuchi-Storm, Nao, Moakes, Simon, Thüer, Susann, Grovermann, Christian, Verwer, Cynthia, Verkaik, Jan, Knubben-Schweizer, Gabriela, Höglund, Johan, Petkevičius, Saulius, Thamsborg, Stig, and Werne, Steffen
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- 2019
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8. Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method
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Prakas, Petras, Strazdaitė-Žielienė, Živilė, Januškevičius, Vytautas, Chiesa, Francesco, Baranauskaitė, Agnė, Rudaitytė-Lukošienė, Eglė, Servienė, Elena, Petkevičius, Saulius, and Butkauskas, Dalius
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- 2020
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9. Dirofilaria repens in dogs and humans in Lithuania
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Sabūnas, Vytautas, Radzijevskaja, Jana, Sakalauskas, Povilas, Petkevičius, Saulius, Karvelienė, Birutė, Žiliukienė, Jolanta, Lipatova, Indrė, and Paulauskas, Algimantas
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- 2019
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10. Does the in vitro egg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus?
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Babják Michal, Königová Alžbeta, Urda Dolinská Michaela, Kupčinskas Tomas, Vadlejch Jaroslav, von Samson-Himmelstjerna Georg, Petkevičius Saulius, and Várady Marián
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anthelmintic resistance ,detection methods ,egg hatch test ,haemonchus contortus ,goats ,Infectious and parasitic diseases ,RC109-216 - Abstract
Considerable research has been directed towards optimising in vitro tests that can diagnose resistance in pre-parasitic stages of parasites. The objective of this study was to compare the in vivo faecal egg count reduction test (FECRT), the in vitro egg hatch test (EHT), and the molecular determination of the frequency of a codon 200 allele of β-tubulin isotype 1 associated with benzimidazole resistance in larval stages of Haemonchus contortus obtained from infected goats. Animals were infected with composite infective doses representing 10, 20, 30, 40, 60, and 80% resistant alleles. Faecal samples for the EHT were collected on 28, 33, and 35 days post-infection. The results of the in vivo FECRT indicated that albendazole treatment reduced infections consisting of composite doses of 10, 20, 30, 40, 60, and 80% larvae of the resistant isolate by 91.3, 78.0, 63.3, 48.4, 36.5, and 41.4%, respectively. The drug concentration at which 50% of the eggs were prevented from developing hatching larvae (ED50) in the in vitro EHT varied from 0.09 ± 0.01 to 15.63 ± 12.10 μg/mL thiabendazole. The results of the in vitro EHT indicated that the test could estimate in vivo resistance well. The EHT could thus accurately estimate the in vivo efficacy of the drug and percentage of the resistance allele in the population using hatching parameters in delineation doses. This finding was also supported by comparing the FECRT data to the hatching percentages in the EHT on 30 goat farms in Slovakia with natural mixed infections of gastrointestinal parasites.
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- 2021
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11. Efficacy against nematode and cestode infections and safety of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination product in domestic cats under field conditions in Europe
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Rehbein, Steffen, Capári, Balazs, Duscher, Georg, Keidane, Dace, Kirkova, Zvezdelina, Petkevičius, Saulius, Rapti, Dhimiter, Wagner, Annegret, Wagner, Thomas, Chester, S. Theodore, Rosentel, Joseph, Tielemans, Eric, Visser, Martin, Winter, Renate, Kley, Katrin, and Knaus, Martin
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- 2014
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12. Efficacy against nematode infections and safety of afoxolaner plus milbemycin oxime chewable tablets in domestic dogs under field conditions in Europe
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Rehbein, Steffen, Knaus, Martin, Mallouk, Yasmina, Breiltgens, Tatjana, Brianti, Emanuele, Capári, Balázs, Dantas-Torres, Filipe, Gau, Michel, Joachim, Anja, Kaulfuß, Karl-Heinz, Kirkova, Zvezdelina, Lechner, Joerg, Mihalca, Andrei D., Mirabito, Rosamaria, Petkevičius, Saulius, Rapti, Dhimitër, Shukullari, Enstela, Sedeilhan, Michel, Dollhofer, Doris, Kley, Katrin, Lebon, Wilfried, Visser, Martin, and Jeannin, Philippe
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- 2017
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13. The propensity of voles and mice to transmit Borrelia burgdorferi sensu lato infection to feeding ticks
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Radzijevskaja, Jana, Paulauskas, Algimantas, Rosef, Olav, Petkevičius, Saulius, Mažeika, Vytautas, and Rekašius, Tomas
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- 2013
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14. Fish helminths in Lithuanian inner waters
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Mažeika, Vytautas, primary, Petkevičius, Saulius, additional, Pumputis, Egidijus, additional, and Krikštolaitis, Ričardas, additional
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- 2022
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15. Reducing antimicrobial use in poultry farming
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Molteni, Roberto, Lévêque Gérard, Jansson Désirée, Hamina Hanna, Sparks Nicholas, Cerdà-Cuéllar Marta, Gefeller Eva-Maria, Margaret, Hardy, Koopman Rik, Scerri Karl, Delezie Evelyne, Ribó Arboledas Oriol, Suojala Leena, Kreyenbühl Karin, Monica, Guarino Amato, Bruno, Roque, Petkevičius Saulius, Harmandjiev Philip, Molnar Daniel, Christensen Laurids Siig, and Manzanilla, Edgar Garcia
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- 2021
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16. Additional file 1 of Anthelmintic resistance of horse strongyle nematodes to ivermectin and pyrantel in Lithuania
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Dauparaitė, Evelina, Kupčinskas, Tomas, Samson-Himmelstjerna, Georg Von, and Petkevičius, Saulius
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fluids and secretions ,embryonic structures ,digestive, oral, and skin physiology - Abstract
Additional file 1. The mean pre-treatment and post-treatment faecal egg count, faecal egg count reduction percentage, and lower and upper 95% confidence limits for each group of anthelmintic drugs tested.
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- 2021
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17. A Qualitative Market Analysis Applied to Mini-FLOTAC and Fill-FLOTAC for Diagnosis of Helminth Infections in Ruminants
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Maurelli, Maria Paola, primary, Dourado Martins, Oliva Maria, additional, Morgan, Eric R., additional, Charlier, Johannes, additional, Cringoli, Giuseppe, additional, Mateus, Teresa Letra, additional, Bacescu, Bogdan, additional, Chartier, Christophe, additional, Claerebout, Edwin, additional, de Waal, Theo, additional, Helm, Christina, additional, Hertzberg, Hubertus, additional, Hinney, Barbara, additional, Höglund, Johan, additional, Kyriánová, Iveta Angela, additional, Mickiewicz, Marcin, additional, Petkevičius, Saulius, additional, Simin, Stanislav, additional, Sotiraki, Smaragda, additional, Tosheska, Marina, additional, Toth, Mariann, additional, Martínez-Valladares, María, additional, Varady, Marian, additional, Sekovska, Blagica, additional, von Samson-Himmelstjerna, Georg, additional, and Rinaldi, Laura, additional
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- 2020
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18. A qualitative market analysis applied to Mini-FLOTAC and Fill-FLOTAC for diagnosis of helminth infections in ruminants
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Maurelli, Maria Paola, Dourado Martins, Oliva Maria, Morgan, Eric R, Charlier, Johannes, Cringoli, Giuseppe, Mateus, Teresa Letra, Bacescu, Bogdan, Chartier, Christophe, Claerebout, Edwin, de Waal, Theo, Helm, Christina, Hertzberg, Hubertus, Hinney, Barbara, Höglund, Johan, Kyriánová, Iveta Angela, Mickiewicz, Marcin, Petkevičius, Saulius, Simin, Stanislav, Sotiraki, Smaragda, Tosheska, Marina, Toth, Mariann, Martínez-Valladares, María, Varady, Marian, Sekovska, Blagica, von Samson-Himmelstjerna, Georg, Rinaldi, Laura, Maurelli, Maria Paola, Dourado Martins, Oliva Maria, Morgan, Eric R, Charlier, Johannes, Cringoli, Giuseppe, Mateus, Teresa Letra, Bacescu, Bogdan, Chartier, Christophe, Claerebout, Edwin, de Waal, Theo, Helm, Christina, Hertzberg, Hubertus, Hinney, Barbara, Höglund, Johan, Kyriánová, Iveta Angela, Mickiewicz, Marcin, Petkevičius, Saulius, Simin, Stanislav, Sotiraki, Smaragda, Tosheska, Marina, Toth, Mariann, Martínez-Valladares, María, Varady, Marian, Sekovska, Blagica, von Samson-Himmelstjerna, Georg, and Rinaldi, Laura
- Abstract
Helminth infections, mainly by gastrointestinal nematodes (GIN), are one of the main concerns for animal health, welfare and productivity in grazing ruminant livestock worldwide. The use of a sensitive, precise, accurate, low-cost, and easy-to-perform copromicroscopic technique is of pivotal importance to perform reliable fecal egg count (FEC) and fecal egg count reduction test (FECRT), in order to determine the need of anthelmintic treatment, but also anthelmintic efficacy or resistance. This approach is fundamental to a correct and efficient control of GIN. Unfortunately, in worldwide ruminant farm practice, repeated anthelmintic treatments are carried out, without prior diagnosis of infection, contributing to the spread of Anthelmintic Resistance (AR). Tackling this phenomenon, improving mainly the GIN diagnosis and AR status in farm animals, is a priority of the European COST Action "COMBAR-COMBatting Anthelmintic Resistance in Ruminants" and of the STAR-IDAZ International Research Consortium on Animal Health. One of the specific objectives of the COMBAR Working Group 1 (WG1) is to conduct an European market analysis of new diagnostics and develop a business plan for commercial test introduction, leveraging technical know-how of participants. Since the Mini-FLOTAC in combination with the Fill-FLOTAC may be considered a good candidate for a standardized FEC and FECRT in the laboratory, as well as directly in the field, the aim of this study was to conduct SWOT (Strength-Weaknesses-Opportunities-Threats) and PESTEL (Political, Economic, Social, Technological, Environmental, and Legal) analyses of these tools in 20 European countries involved in the COMBAR WG1, in order to identify the opportunities, barriers, and challenges that might affect the Mini-FLOTAC and Fill-FLOTAC commercialization in Europe.
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- 2020
19. Prevalence and intensity of Sarcocystis spp. infection in animals slaughtered for food in Lithuania
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Januskevicius, Vytautas, Januskeviciene, Grazina, Prakas, Petras, Butkauskas, Dalius, and Petkevičius, Saulius
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sheep ,infection intensity ,cattle ,infection prevalence ,pigs ,horses - Abstract
The exact prevalence of Sarcocystis spp. infection in animals slaughtered for food is unknown in Lithuania. Therefore, the present study was initiated to evaluate Sarcocystis spp. infection in the carcasses of cattle (n = 206), sheep (n = 61), pigs (n = 73) and horses (n = 72) raised in Lithuania for food. The prevalence and intensity of Sarcocystis spp. infection were assessed under light microscopy by analysing 1 g of stained and squashed muscle samples. All the investigated muscle types (oesophagus, diaphragm, heart, neck, jaw, back, leg and tongue) were found to have been infected with microcysts rather than with macrocysts. A high prevalence of infection was established in cattle (44.9–98.1%) and sheep (100%), whereas the prevalence of this infection in pigs (30.1–50.0%) and horses (34.7–63.9%) was considered to be moderate. Significant differences in the infection prevalence were detected in the majority of muscle groups of cattle and in some muscle groups of pigs and horses. Similarly, significant differences in the median (Md) intensity of infection were observed in the majority of the muscle groups of cattle (Md = 4–29) and sheep (Md = 21–73) and only in some muscle groups of pigs (Md = 4.5–16) and horses (Md = 1–3). Cases of intense infection (> 40 cysts in a sample) were relatively often detected in sheep (44.9%) and cattle (19.1%), and rarely in pigs (3.7%). Hence, based on the varying rates of infection in the examined samples, the infection was identified as being intense in sheep and cattle, moderate in pigs and low in horses.
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- 2019
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20. Worm control on organic farms in the EU: Management and the farmers’ point of view
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Werne, Steffen, Moakes, Simon, Hoste, Herve, Verkaik, Jan, Verwer, Cynthia, Petkevičius, Saulius, and Athanasiadou, Spiridoula
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Sheep and goats ,Health and welfare - Abstract
A structured survey was developed to determine farmers’ disposition towards recently developed refugia-based concepts. The focus was on individual drenching, possible increased workload or costs and reduced production that might come with innovations. Furthermore, the survey aimed at assessing the status-quo of gastrointestinal nematode (GIN) control on organic small ruminant farms in 5 European countries. In total 441 surveys of organic certified small ruminant farms were evaluated, of which were 129 dairy goat and 320 meat sheep surveys. Amongst other things, the survey covered applied alternatives such as feeding bioactive forage, increased protein supply, phytotherapy, homeopathy and culling of nematode-susceptible animals. The number of drenches per animal per year in dairy goats varied from 1.3 (CH) to 1.6 (FR), in sheep from 0.8 (LT) to 1.5 (CH) and in lambs from 0.9 (LT) to 1.6 (CH). Applied individual drenching of lambs was relatively low from 2% (LT) to 33% (UK). Sixteen percent (NL) to 36% (CH) of the farmers drenched their dairy goats individually. Eight percent (UK) to 49% (LT) did not drench their lambs at all. Seven percent (CH) to 21% (FR) of the organic farmers did not apply any anthelmintic to their dairy goats. Between 10% (LT) and 40% (NL) of the farmers used faecal sampling to monitor GIN, whereas performance parameters (weight gain or milk yield) were moderately used from 19% (CH) to 41% (LT). The use of phytotherapy varied considerably between livestock species and country from 13% (LT) – 69% (CH). The majority of farmers rather or fully agreed that anthelmintic resistance will worsen in future (51% – 92%). When asked about performance losses that may be caused by the introduction of alternative methods, the agreement was more variable with 7% in NL to 93% in LT. Our data suggest that innovations might be accepted despite higher labor input or costs. As some organic farmers make already use of phenotypic traits like production loss, it should not be a big step towards a “Targeted Selective Treatment” approach for farmers that are open minded towards innovations.
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- 2019
21. Parasite control in organic cattle farming:Management and farmers' perspectives from six European countries
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Takeuchi-Storm, Nao, Moakes, Simon, Thüer, Susann, Grovermann, Christian, Verwer, Cynthia, Verkaik, Jan, Knubben-Schweizer, Gabriela, Höglund, Johan, Petkevičius, Saulius, Thamsborg, Stig, Werne, Steffen, Takeuchi-Storm, Nao, Moakes, Simon, Thüer, Susann, Grovermann, Christian, Verwer, Cynthia, Verkaik, Jan, Knubben-Schweizer, Gabriela, Höglund, Johan, Petkevičius, Saulius, Thamsborg, Stig, and Werne, Steffen
- Abstract
Organic ruminant production is expanding in the EU, but parasite management remains a constant challenge. Mandatory outdoor access for all age groups can increase exposure to pasture borne parasites, whilst restrictions in the prophylactic use of anthelmintics can limit parasite control. The scientific community has been working to deliver effective parasite control strategies and alternative approaches in order to slow down the development of anthelmintic resistance (AR). However, the current parasite control practices and overall awareness with regards to AR and alternative approaches on farms are largely unknown and may be causing a knowledge gap between the scientific and farming communities. Therefore, a structured survey was conducted in six European countries (Switzerland, Germany, Denmark, Netherlands, Lithuania, Sweden) to provide basic data on practices, management and farmers' perspectives for grazing and parasite control (gastrointestinal worms and liver flukes) on organic cattle farms. Overall, 375 surveys were collected (282 dairy and 93 beef farms) in 2015–2016, and analysed descriptively. Additionally, surveys from the 228 dairy farms were assessed using a double-hurdle adoption model to identify the factors involved in the decision to drench against gastrointestinal parasites. Generally, there are prominent differences between countries, with monitoring methods differing especially, which has important implications in terms of knowledge transfer. For example, media warning was the most common method in DE, while antibody testing in bulk tank milk was the common method in NL. In other countries, clinical signs (diarrhoea, hair coat quality, and reduced weight or yield) and liver condemnation data were used frequently. In general, organic farmers from the six participating countries indicated that they would accept alternative approaches despite greater cost and labour. The likelihood of drenching were higher on farms with smaller farm areas, highe
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- 2019
22. Detection and molecular characterization of canine babesiosis causative agent Babesia canis in the naturally infected dog in Lithuania
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Paulauskas, Algimantas, Radzijevskaja, Jana, Karvelienė, Birutė, Grigonis, Aidas, Aleksandravičienė, Asta, Zamokas, Gintaras, Babickaitė, Lina, Sabūnas, Vytautas, and Petkevičius, Saulius
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- 2014
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23. Efficacy against nematode infections and safety of afoxolaner plus milbemycin oxime chewable tablets in domestic dogs under field conditions in Europe
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Rehbein, Steffen, primary, Knaus, Martin, additional, Mallouk, Yasmina, additional, Breiltgens, Tatjana, additional, Brianti, Emanuele, additional, Capári, Balázs, additional, Dantas-Torres, Filipe, additional, Gau, Michel, additional, Joachim, Anja, additional, Kaulfuß, Karl-Heinz, additional, Kirkova, Zvezdelina, additional, Lechner, Joerg, additional, Mihalca, Andrei D., additional, Mirabito, Rosamaria, additional, Petkevičius, Saulius, additional, Rapti, Dhimitër, additional, Shukullari, Enstela, additional, Sedeilhan, Michel, additional, Dollhofer, Doris, additional, Kley, Katrin, additional, Lebon, Wilfried, additional, Visser, Martin, additional, and Jeannin, Philippe, additional
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- 2016
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24. Stable isotope ratio method for the characterisation of the poultry house environment
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Skipitytė, Raminta, primary, Mašalaitė, Agnė, additional, Garbaras, Andrius, additional, Mickienė, Rūta, additional, Ragažinskienė, Ona, additional, Baliukonienė, Violeta, additional, Bakutis, Bronius, additional, Šiugždaitė, Jūratė, additional, Petkevičius, Saulius, additional, Maruška, Audrius Sigitas, additional, and Remeikis, Vidmantas, additional
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- 2016
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25. Histological and immunohistochemical practical studies of canine cutaneous tumors
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Šimkus, Donatas, primary, Petkevičius, Saulius, additional, Pridotkas, Gediminas, additional, Zorgevica-Pockeviča, Ligita, additional, Maskaliovas, Viktoras, additional, Šimkienė, Virginija, additional, and Pockevičius, Alius, additional
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- 2016
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26. Prevalence of anthelmintic resistance on Lithuanian sheep farms assessed by in vitro methods
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Kupčinskas, Tomas, primary, Stadalienė, Inga, additional, Šarkūnas, Mindaugas, additional, Riškevičienė, Vita, additional, Várady, Marian, additional, Höglund, Johan, additional, and Petkevičius, Saulius, additional
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- 2015
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27. Seasonal patterns of gastrointestinal nematode infection in goats on two Lithuanian farms
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Stadalienė, Inga, primary, Höglund, Johan, additional, and Petkevičius, Saulius, additional
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- 2015
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28. Galvijų infekcinio rinotracheito ir virusinės diarėjos diagnostikos ir prevencijos problemos
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Šalomskas, Algirdas, Žaliūnaitė, Violeta, Jacevičius, Eugenijus, Lelešius, Raimundas, Mockeliūnas, Raimundas, Kliučinskas, Rolanas, and Petkevičius, Saulius
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Virus diseases--Diagnosis ,Cows--Viral diarrhea ,Virusinės ligos--Diagnostika ,viruses ,Iinfectious bovine rhinotracheitis ,Bovine viral diarrhea ,Dagnosis ,Polymerase chain reaction ,animal diseases ,Karvės--Virusinės ligos ,Karvės--Virusinė diarėja ,Cows--Virus diseases ,636.2 [udc] - Abstract
Darbo tikslas buvo ištirti galvijų infekcinio rinotracheito (GIR) epizootologinę situaciją kai kuriose veislinėse bandose, įvertinti polimerazės grandininės reakcijos galimybes diagnozuojant ūmią galvijų. The study was designed to estimate the distribution of infectious bovine rhinotracheitis (IBR) and bovine viral diarhhoea (BVD) in randomly selected high productive cattle herds in Lithuania. For this purpose the polymerase chain reaction (PCR) for detection of bovine herpesvirus type 1 (BHV-1) in the case of calves acute respiratory disorders was implemented, the one tube nested PCR for detection of BVD virus persistently infected (PI) animals was evaluated, and the role of PI animals on the rate of BVD virus spread within the herd was estimated. This study demonstrated that in investigated nine cattle herds 45.9% of animals were seropositive to BHV-1. It was statistically higher amount of seropositive animals among cows compared to the heifers (P
- Published
- 2006
29. Stable isotope ratio method for the characterisation of the poultry house environment.
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Skipitytė, Raminta, Mašalaitė, Agnė, Garbaras, Andrius, Mickienė, Rūta, Ragažinskienė, Ona, Baliukonienė, Violeta, Bakutis, Bronius, Šiugždaitė, Jūratė, Petkevičius, Saulius, Maruška, Audrius Sigitas, and Remeikis, Vidmantas
- Subjects
OXYGEN isotopes ,POULTRY housing ,ANIMAL health - Abstract
Stable isotope analysis was applied to describe the poultry house environment. The poultry house indoor environment was selected for this study due to the relevant health problems in animals and their caretakers. Air quality parameters including temperature, relative humidity, airflow rate, NH3, CO2and total suspended particles, as well as mean levels of total airborne bacteria and fungi count, were measured. Carbon isotope ratios (13C/12C) were obtained in size-segregated aerosol particles. The carbon (13C/12C) and nitrogen (15N/14N) isotope ratios were measured in feed, litter, scrapings from the ventilation system, feathers and eggs. Additionally, the distribution ofδ13C andδ15N values in different tissues of the chicken was examined. The airborne bacteria and fungi extracted from the air filters collected from poultry farms were grown in the laboratory in media with known isotope values and measured for stable isotope ratios. Analysis of isotope fractionation between microorganisms and their media indicated the applicability of stable isotope analysis in bulk samples for the identification of source material. The analysed examples imply that stable isotope analysis can be used to examine the indoor environment along with its biology and ecology, and serve as an informative bioanalytical tool. [ABSTRACT FROM AUTHOR]
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- 2017
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30. Arklių OCD (osteochondritis dissecans) kulno sąnario rentgeninių pakitimų analizė
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Jokimas, Juozas, Laurinavičiūtė, Vilija, Kučinskas, Audrius, Petkevičius, Saulius, Bižokas, Vidmantas, Vaitkus, Valdas, Gintautas, Jonas, Černius, Romas, Maslauskas, Kęstutis, Lithuanian University of Health Sciences, Džiaugytė, Emilija, Jokimas, Juozas, Laurinavičiūtė, Vilija, Kučinskas, Audrius, Petkevičius, Saulius, Bižokas, Vidmantas, Vaitkus, Valdas, Gintautas, Jonas, Černius, Romas, Maslauskas, Kęstutis, Lithuanian University of Health Sciences, and Džiaugytė, Emilija
- Abstract
Tyrimas buvo atliekamas Lietuvos sveikatos mokslų universiteto, Veterinarijos akademijos, Stambiųjų gyvulių klinikoje. Pasirinktas tyrimo objektas, šlubuojančių arklių kulno sąnarys; metodika pirminis klinikinis tyrimas bei rentgenologinis tyrimas. Tiriamoji arklių grupė buvo sudaryta iš arklių, kurie buvo pasirinkti atsitiktiniu būdu. Įvairių veislių, lyčių ir amžiaus. Arklių kulno sąnarys yra vienas iš judamojo aparato – sudėtingas mechanizmas. Sudarytas iš kelių kaulų junginio ir sudaro vienaašį sudėtinį sąnarį (sraigtinis/sudėtinis). Blauzdikaulio, kulnies ir pėdos kaulų junginys. Šis sudėtinis judamasis mechanizmas dažnai paveikiamas Osteochondritis dissecans patologijos, kuris kelia arkliams skausmingą diskomforto jausmą ir gyvulys į šį dirgiklį atsako šlubavimu. Arklių kulno sąnario Osteochondritis dissecans dažniausiai diagnozuojamas sportiniams žirgams (56,53 proc.) dėl neadekvataus gyvulio jėgų išnaudojimo arba spartaus kumeliuko augimo ir nesubalansuoto jam tinkamo raciono. Taip pat nustatyta, jog kulno sąnario OCD diagnozuojama eržilams/kastratams (75 proc.) daugiau, nei kumelėms (25 proc.). Tyrimo metu paaiškėjo, jog kulno sąnario OCD pažeidimai lokalizavosi tiek pas eržilus/kastratus, tiek kumeles, daugiausiai kairiajame kulno sąnaryje (58,33 proc.), o dešinės kulno sąnario kojos pažeidimų aptikta procentine išraiška mažiau (33,33 proc.)., The study was conducted in Lithuanian University of Health Sciences, Veterinary Academy, Large Animal Clinic. The selected object of research was lame horse hock joint; method - initial clinical examination and radiographic analysis. The exploratory group of equines consisted of horses which were selected incidentally: various breeds, genders and age. Horse hock joint is one of the apparatus of locomotion which is a complex mechanism. It is composed of a compound of several bones to form a one-axis composite joint (screw/composite). It consists of combination of tibia, hock and foot bones. This complex locomotor mechanism is often affected by the pathology of Osteochondritis dissecans which causes painful feeling of discomfort for horses and consequently, they respond to this irritant by lameness. Horse Osteochondritis dissecans most often is diagnosed for racing and sports horses (56.53 percent) due to inadequate exploitation of animal strength, rapid growth of foal and improper diet. It was also found that OCD was diagnosed in stallions/geldings (75 percent) more than in mares (25 percent). The study revealed that OCD lesions localized to both stallions/geldings and mares, mostly on the left hock joint (58.33 percent), while the lesions of hock joint of the right leg were detected by a percentage less (33.33 percent).
- Published
- 2014
31. Kiaulių virškinamojo trakto parazitų paplitimas Kėdainių rajone
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Mockeliūnas, Raimundas, Juodsnukytė, Zita, Žilaitis, Vytuolis, Bižokas, Vidmantas, Stankevičienė , Marija, Jokimas, Juozas, Antanaitis, Ramūnas, Malakauskas , Alvydas, Petkevičius, Saulius, Lithuanian University of Health Sciences, Parapijonavičius, Vytautas, Mockeliūnas, Raimundas, Juodsnukytė, Zita, Žilaitis, Vytuolis, Bižokas, Vidmantas, Stankevičienė , Marija, Jokimas, Juozas, Antanaitis, Ramūnas, Malakauskas , Alvydas, Petkevičius, Saulius, Lithuanian University of Health Sciences, and Parapijonavičius, Vytautas
- Abstract
Darbo tikslas: Nustatyti virškinimo trakto helmintų populiacijos paplitimą Kėdainių rajone. Uždaviniai: Atlikti analizę tarp smulkių kiaulių augintojų ir vidutinės kiaulių fermos. Tyrimo metodika. Mėginiai buvo imami iš smulkių Kėdainių rajono, Kalnaberžės, Sirutiškio, Berželės kaimų, kiaulių augintojų, kurie laiko iki 10 gyvulių ir iš vidutinės „X“ kiaulių fermos (apie 400 kiaulių). Buvo palygintas invazijos intensyvumas skirtingo dydžio fermose. Tyrimai atliekami 2012 m., balandžio - birželio ir spalio - lapkričio mėnesiais. Gyvuliai buvo suskirstyti pagal amžių į grupes: 0 – 3 mėn., 3 – 4 mėn., 4 – 7 mėn., 9 – 11 mėn. Iš viso buvo paimta 47 kiaulių mėginiai iš smulkių ūkininkų ir 47 mėginiai iš ,, X ‘‘ kiaulių fermos. Iš viso surinkti 94 mėginiai. Tyrimai buvo atlikti Lietuvos sveikatos ir mokslų universiteto, Veterinarijos akademijos, Užkrečiamųjų ligų katedroje, parazitologijos laboratorijoje modifikuotu MacMasterio metodu. Rezultatai. Nustatyta, kad, 0-3 mėn. paršų grupėje 75,0 proc. ir 25,0 proc., gyvulių, smulkių augintojų ir vidutinio dydžio fermoje, buvo užsikrėtę Eimeria spp. oocistomis. Tyrimai parodė, kad 5-8 mėn. amžiaus grupėje 55,55 proc. ir 10,0 proc., gyvulių, smulkių augintojų ir vidutinio dydžio fermoje, buvo užsikrėtę A. suum helmintais. Tyrimais daugeliu atvejų nustatyta silpna askaridžių invazija, nes mėginiuose rasta tik po 20-40 askaridžių kiaušinėlių 1 g išmatų. Nustatyta, kad 8-11 mėn. amžiaus grupėje 83,33 proc. ir 33,33 proc., gyvulių, o... [toliau žr. visą tekstą], The objectives of study: To determine the prevalence of pigs gastrointestinal parasites in district of Kėdainiai. Purpose of study. To compare the prevalence of parasitic infection in small and medium-sized pigs farms. Materials and methods. The samples were collected from small farms (1 – 10 pigs) in district of Kėdainiai, villages of Kalnaberžės, Sirutiškio, Berželės and from medium-sized „X“ farm (appr.y 400 pigs). The assessment of the infection intensity in different size farms was performed. The analysis was done from April to June and from October to November of year 2012. The animals were divided into groups according to the age: 0 – 3 months, 3 – 4 months, 4 – 7 months and 9 – 11 months. In total 47 samples were collected from small farms and 47 samples were collected from middle size „X“ farm. As a result, totally were selected 94 samples. For estimation of parasitic infexction level a modified McMaster technique was used. Results. 75 % of pigs in small farms and 25 % of animals in medium-sized farms, in group of 0 – 3 months age, were infected with Eimeria spp. oocysts. Furthermore, 55,55 % of pigs in small farms and 10,0 % of animals in medium-sized farms, in group of 5 – 8 months age, were infected with A. suum helminths. The low invasion of ascarids was found in severalcases, because EPG was only 20 – 40 ascarids eggs.. In addition, 83,33 % of pigs in small farms and 33,33 % of animals in medium-sized farms, in group of 8 – 11 months age, were... [to full text]
- Published
- 2013
32. Diversity and ecology of Sarcocystis in Lithuanian game fauna
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Valkiūnas, Gediminas, Žalakevičius, Mečislovas, Lazutka, Juozas Rimantas, Stonis, Jonas Rimantas, Paulauskas, Algimantas, Petkevičius, Saulius, Palinauskas, Vaidas, Vilnius University, Prakas, Petras, Valkiūnas, Gediminas, Žalakevičius, Mečislovas, Lazutka, Juozas Rimantas, Stonis, Jonas Rimantas, Paulauskas, Algimantas, Petkevičius, Saulius, Palinauskas, Vaidas, Vilnius University, and Prakas, Petras
- Abstract
Up till now ecology and biodiversity of Sarcocystis species in game fauna in Lithuania has been investigated using traditional morphological methods. In the period of 2005-2011, muscle samples of 384 birds and 177 mammals were examined for Sarcocystis sarcocysts. Cysts of Sarcocystis spp. were investigated using light microscopy, transmission electron microscopy and DNA analysis (18S rDNA, 28S rDNA, ITS–1 region). Statistically significant higher Sarcocystis infection prevalence and intensity rates (p< 0.05) were determined in mammals as compared to the birds. Macrocysts were detected only in the mallard, they were identified as S. rileyi and this is the first evidence of S. rileyi infection in Europe. Based on results of cyst wall ultrastructure and DNA analysis four new bird Sarcocystis species were described: S. albifronsi, S. wobeseri, S. anasi, S. cornixi. Eight Sarcocystis species were identified in the examined mammals using morphological and DNA analysis: S. miescheriana from wild boar; S. gracilis, S. capreolicanis, S. oviformis, S. silva and S. hofmanni-like from roe deer; S. hjorti, S. hofmanni-like and Sarcocystis sp. ex Cervus elaphus from red deer; S. hjorti from moose. S. columbae, S. oviformis, S. hjorti and S. silva were found in Lithuania for the first time. Using molecular investigation it was proved that some analyzed Sarcocystis species (S. wobeseri, S. hjorti, S. silva and S. hofmanni-like) are not rigidly specific to the intermediate host. Sarcocystis... [to full text], Lietuvoje medžiojamosios faunos Sarcocystis rūšių ekologija ir bioįvairovė iki šiol tirta naudojant tradicinius morfologinius metodus. 2005-2011 metais ieškant Sarcocystis sarkocistų Lietuvos medžiojamoje faunoje analizuoti 384 paukščių ir 177 žinduolių raumenų pavyzdžiai. Sarcocystis cistos tirtos naudojant šviesinės ir elektroninės mikroskopijos metodus bei DNR žymenis (18S rDNR, 28S rDNR, ITS–1 regionas). Lyginant paukščių ir žinduolių sistematines grupes, žinduoliuose nustatyti patikimai (p< 0,05) didesni Sarcocystis infekcijos ekstensyvumo bei intensyvumo rodikliai. Makrocistos aptiktos tik didžiojoje antyje ir buvo priskirtos S. rileyi rūšiai – tai pirmas svarus S. rileyi infekcijos įrodymas Europoje. Remiantis cistų sienelės ultrastruktūros ir DNR tyrimo duomenimis aprašytos keturios naujos mokslui paukščių sarkosporidijų rūšys: S. albifronsi, S. wobeseri, S. anasi, S. cornixi. Naudojant morfologinius ir DNR tyrimo metodus tirtuose žinduoliuose identifikuotos aštuonios Sarcocystis rūšys: šernuose S. miescheriana; stirnose S. gracilis, S. capreolicanis, S. oviformis, S. silva, S. hofmanni-like; tauriuosiuose elniuose S. hjorti, S. hofmanni-like, Sarcocystis sp. ex Cervus elaphus ir briedžiuose S. hjorti. Šio darbo metu pirmą kartą Lietuvoje aptiktos S. columbae, S. oviformis, S. hjorti, S. silva rūšys. Molekuliniais tyrimais įrodyta, kad kai kurios tirtos Sarcocystis rūšys (S. wobeseri, S. hjorti, S. silva, S. hofmanni-like) nėra griežtai specifinės tarpiniam... [toliau žr. visą tekstą]
- Published
- 2011
33. Lietuvos medžiojamosios faunos sarkosporidijų (Sarcocystis) įvairovė ir ekologija
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Valkiūnas, Gediminas, Žalakevičius, Mečislovas, Lazutka, Juozas Rimantas, Stonis, Jonas Rimantas, Paulauskas, Algimantas, Petkevičius, Saulius, Palinauskas, Vaidas, Vilnius University, Prakas, Petras, Valkiūnas, Gediminas, Žalakevičius, Mečislovas, Lazutka, Juozas Rimantas, Stonis, Jonas Rimantas, Paulauskas, Algimantas, Petkevičius, Saulius, Palinauskas, Vaidas, Vilnius University, and Prakas, Petras
- Abstract
Lietuvoje medžiojamosios faunos Sarcocystis rūšių ekologija ir bioįvairovė iki šiol tirta naudojant tradicinius morfologinius metodus. 2005-2011 metais ieškant Sarcocystis sarkocistų Lietuvos medžiojamoje faunoje analizuoti 384 paukščių ir 177 žinduolių raumenų pavyzdžiai. Sarcocystis cistos tirtos naudojant šviesinės ir elektroninės mikroskopijos metodus bei DNR žymenis (18S rDNR, 28S rDNR, ITS–1 regionas). Lyginant paukščių ir žinduolių sistematines grupes, žinduoliuose nustatyti patikimai (p< 0,05) didesni Sarcocystis infekcijos ekstensyvumo bei intensyvumo rodikliai. Makrocistos aptiktos tik didžiojoje antyje ir buvo priskirtos S. rileyi rūšiai – tai pirmas svarus S. rileyi infekcijos įrodymas Europoje. Remiantis cistų sienelės ultrastruktūros ir DNR tyrimo duomenimis aprašytos keturios naujos mokslui paukščių sarkosporidijų rūšys: S. albifronsi, S. wobeseri, S. anasi, S. cornixi. Naudojant morfologinius ir DNR tyrimo metodus tirtuose žinduoliuose identifikuotos aštuonios Sarcocystis rūšys: šernuose S. miescheriana; stirnose S. gracilis, S. capreolicanis, S. oviformis, S. silva, S. hofmanni-like; tauriuosiuose elniuose S. hjorti, S. hofmanni-like, Sarcocystis sp. ex Cervus elaphus ir briedžiuose S. hjorti. Šio darbo metu pirmą kartą Lietuvoje aptiktos S. columbae, S. oviformis, S. hjorti, S. silva rūšys. Molekuliniais tyrimais įrodyta, kad kai kurios tirtos Sarcocystis rūšys (S. wobeseri, S. hjorti, S. silva, S. hofmanni-like) nėra griežtai specifinės tarpiniam... [toliau žr. visą tekstą], Up till now ecology and biodiversity of Sarcocystis species in game fauna in Lithuania has been investigated using traditional morphological methods. In the period of 2005-2011, muscle samples of 384 birds and 177 mammals were examined for Sarcocystis sarcocysts. Cysts of Sarcocystis spp. were investigated using light microscopy, transmission electron microscopy and DNA analysis (18S rDNA, 28S rDNA, ITS–1 region). Statistically significant higher Sarcocystis infection prevalence and intensity rates (p< 0.05) were determined in mammals as compared to the birds. Macrocysts were detected only in the mallard, they were identified as S. rileyi and this is the first evidence of S. rileyi infection in Europe. Based on results of cyst wall ultrastructure and DNA analysis four new bird Sarcocystis species were described: S. albifronsi, S. wobeseri, S. anasi, S. cornixi. Eight Sarcocystis species were identified in the examined mammals using morphological and DNA analysis: S. miescheriana from wild boar; S. gracilis, S. capreolicanis, S. oviformis, S. silva and S. hofmanni-like from roe deer; S. hjorti, S. hofmanni-like and Sarcocystis sp. ex Cervus elaphus from red deer; S. hjorti from moose. S. columbae, S. oviformis, S. hjorti and S. silva were found in Lithuania for the first time. Using molecular investigation it was proved that some analyzed Sarcocystis species (S. wobeseri, S. hjorti, S. silva and S. hofmanni-like) are not rigidly specific to the intermediate host. Sarcocystis... [to full text]
- Published
- 2011
34. Funkcine dispepsija sergančių pacientų skrandžio gleivinės histomorfologiniai pakitimai bei jų dinamika vartojant augalinės kilmės antioksidantą astaksantiną
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Kupčinskas , Limas, Lesauskaitė , Vaiva, Tamelis , Algimantas, Adamonis , Kęstutis, Didžiapetrienė , Janina, Irnius , Algimantas, Valančiūtė , Angelija, Ivanauskas , Audrius, Petkevičius , Saulius, Lithuanian University of Health Sciences, Jančiauskas, Dainius, Kupčinskas , Limas, Lesauskaitė , Vaiva, Tamelis , Algimantas, Adamonis , Kęstutis, Didžiapetrienė , Janina, Irnius , Algimantas, Valančiūtė , Angelija, Ivanauskas , Audrius, Petkevičius , Saulius, Lithuanian University of Health Sciences, and Jančiauskas, Dainius
- Abstract
Darbo tikslas - įvertinti sergančiųjų funkcine dispepsija skrandžio gleivinės morfologinius pakitimus bei jų pokyčius gydant skirtingomis augalinės kilmės antioksidanto astaksantino dozėmis. Tikslui pasiekti buvo nustatyti ir išspręsti šie uždaviniai: įvertinti infekuotumą Helicobacter pylori mikroorganizmais bei skrandžio gleivinės histomorfologinius pakitimus pagal Sidnėjaus ir OLGA klasifikacijas sergantiesiems funkcine dispepsija; ištirti funkcine dispepsija sergančių ligonių uždegimo žymenų IL-4, IL-6, IL-8, IL-10, IFN-γ bei lastelių žymenų CD4, CD8, CD14, CD19, CD25, CD30 raišką skrandžio gleivinėje; palyginti funkcine dispepsija sergančių ligonių, gydytų augalinės kilmės antioksidanto astaksantino skirtingomis dozėmis ir placebu, skrandžio gleivinės morfologinius pokyčius; palyginti funkcine dispepsija sergančių ligonių, gydytų augalinės kilmės antioksidantu astaksantinu ir placebu, uždegimo žymenų IL-4, IL-6, IL-8, IL-10, IFN-γ bei lastelių žymenų CD4, CD8, CD14, CD19, CD25, CD30 raišką skrandžio gleivinėje., The aim of the study was to evaluate histomorphological changes of the gastric mucosa in functional dyspepsia patients and their dynamics after treatment with the natural antioxidant astaxanthin. The following objectives were established and reached: to determine the presence of Helicobacter pylori infection and evaluate the histomorphological changes of gastric mucosa in functional dyspepsia patients; to evaluate interleukins IL-4, IL-6, IL-8, IL-10 and interferon-γ as well as the cell markers CD4, CD8, CD14, CD19, CD25 and CD30 in functional dyspepsia patients; to evaluate histomorphological changes of the gastric mucosa in functional dyspepsia patients after treatment with the natural antioxidant astaxanthin; to evaluate interleukins IL-4, IL-6, IL-8, IL-10 and interferon-γ as well as the cell markers CD4, CD8, CD14, CD19, CD25 and CD30 in functional dyspepsia patients and their dynamics after treatment with the natural antioxidant astaxanthin.
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- 2010
35. Sporinės bakterijos žaliaviniame piene
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Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Šiugždaitė, Jūratė, Lithuanian Veterinary Academy, Dargytė, Renata, Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Šiugždaitė, Jūratė, Lithuanian Veterinary Academy, and Dargytė, Renata
- Abstract
Darbo tikslas: Išskirti sporinius mikroorganizmus iš žaliavinio pieno. Uždaviniai: 1. Nustatyti sporinius mikroorganizmus iš žaliavinio karvių pieno ir pakratų. 2. Identifikuoti B. cereus, ištirti jų biologines savybes. 3. Palyginti B. cereus paplitimą žaliaviniame piene ir pakratuose. Nors pieno nauda žmogaus organizmui yra visuotinai pripažinta, nepaisant to pienas gali būti ir pavojaus šaltiniu gyvybei ar sveikatai, nes pienas yra puiki terpė mikroorganizmams daugintis. B. cereus gali plačiai paplisti aplinkoje ir užteršti pieno produktus per šiuos šaltinius: žemę, grūdų kultūras ir pieno produktų gamybos priemones. Jų sporos į pieną taip pat patenka nuo nešvaraus tešmens ir spenių. Šiame tyrime buvo įvertintas B. cereus paplitimas žaliaviniame piene ir pakratuose. Atlikti biocheminiai tyrimai, kurių metu, su išskirtomis sporinėmis bakterijomis, buvo atliekamas katalazės nustatymas, gliukozės ir maltozės fermentavimas, judrumo nustatymas, augimas terpėje su 7 % NaCl, atliktos MR ir VP reakcijos, taip pat nitratų redukcijos reakcija, leido patvirtinti išskirtas padermes kaip Bacillus cereus (97 %). Apibendrinus galime teigti, kad svarbiausia mažinti mikrobiologinę taršą žaliaviniame piene, nes B. cereus sporos yra atsparios pasterizacijai, o tai savo ruožtu lemia produktų gedimą ir apsinuodijimo maistu pavojaus padidėjimą., Aim of the study: to extract sporeforming organisms from raw milk. Tasks: to estimate sporeforming organisms from raw milk and bedding; to identify and examine B. cereus and it’s biological features; to compare occurrence of B. cereus in raw milk and bedding; to identify the sensibility of B. cereus to antimicrobial substances. Subject of the study: 369 raw milk samples which where taken in 2008-2009 and 16 samples of bedding which were taken in 2009. There was used MYP & Bacillus cereus selective agar for extraction of sporeforming organisms. To identify B. cereus by examining their biological properties there were used these chemical reactions: catalysis, motility, glucose, maltose, reduction of nitrates, growth in NaCl, methylen red, VP. To examine the sensibility of of B. cereus to antimicrobial substances there were used antibiotic disks: synulox, penicillin, trimethoprim sulphate, cephalexin. There were established that: 4,9% of total raw milk samples were infected by Bacillus cereus. 25% of total bedding samples were infected by Bacillus cereus. Also it was reavealed that there is much bigger chance to find B. cereus in bedding, than in raw milk (p<0,001). 97% of total samples infected by of sporeforming organisms were infected by B. cereus. After testing it was established that 87% of B. cereus strains were resistant to cephalexin, and 87% were sensible to trimethoprim sulphate.
- Published
- 2010
36. Broilerių sparnelių ir blauzdelių užkrėstumas kampilobakterijomis
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Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Malakauskas, Alvydas, Karvelienė, Birutė, Lithuanian Veterinary Academy, Ramonaitė, Sigita, Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Malakauskas, Alvydas, Karvelienė, Birutė, Lithuanian Veterinary Academy, and Ramonaitė, Sigita
- Abstract
Mūsų tyrimo tikslas - įvertinti atšaldytų broilerių produktų (sparnelių ir blauzdelių) užkrėstumą kampilobakterijomis. Tyrimo metu buvo pasirinkti trys paukštienos gamintojai Lietuvoje, kurių gaminiai t.y. broilerių sparneliai bei blauzdelės, jų neįspėjant, buvo perkami parduotuvėse vieną kartą per savaitę, 2009 m. kovo - rugsėjo mėnesių laikotarpyje. Iš viso buvo ištirti 174 paukštienos produktai, iš jų 87 broilerių sparneliai bei 87 blauzdelės. Kiekviename mėginyje termofilinių kampilobakterijų aptikimas buvo atliekamas išskiriant jas tiesiogiai sėjant ant mCCDA agaro bei atlikus pagausinimą Bolton sultinyje. Kampilobakterijų padermių identifikavimas iki rūšies atliktas dauginės polimerazės grandininės reakcijos metodu. Atlikus tyrimą nustatyta, kad termofilinėmis kampilobakterijomis buvo užkrėsti 46,6 % visų tirtų broilerių produktų. Identifikuojant išskirtas kampilobakterijų rūšis, C. jejuni buvo nustatyta 69,12 %, C. coli - 13,23 %, o abi padermės kartu 17,65 % užkrėstų mėginių. Kiekybinio broilerių produktų tyrimo rezultatai parodė, kad vidutinis Campylobacter spp. skaičius tiriant broilerių sparnelius bei blauzdeles buvo 1,56 log10 ksv/g ir 1,53 log10 ksv/g atitinkamai. Šio tyrimo metu nustatytas sąlyginai didelis broilerių produktų užkrėstumas termofilinėmis kampilobakterijomis rodo, kad Lietuvoje parduodami žali broilerių produktai gali sudaryti riziką žmonėms užsikrėsti kampilobakterioze. Todėl siekiant sumažinti vartotojų riziką užsikrėsti kampilobakterioze per... [toliau žr. visą tekstą], The present study was designed to investigate the occurrence and numbers of Campylobacter spp. on broiler chicken wings and drumsticks at the retails level of the three main poultry meat producers in Lithuania. Samples of chicken wings and drumsticks were collected of each poultry meat producer by visiting randomly selected retail shops once a week from March till October, in 2009. A total of 87 chicken wings and 87 drumsticks samples were collected and tested for Campylobacter spp. contamination. Thermophilic Campylobacter spp. were isolated by both direct inoculation on mCCDA selective medium and by selective enrichment in Bolton enrichment broth. Multiplex-PCR method was used for detection and identification of thermophilic Campylobacter species. Our findings showed that overall 46.6 % (81 out of 174) of the collected samples were contaminated with campylobacters. C. jejuni has been found in 69.12 % of the tested samples C. coli in 13.23 % and both species together in 17.65 % respectively. The mean number of Campylobacter bacteria detected on wings at the retail was 1.53 log10 CFU/g and on drumsticks 1.56 log10 CFU/g. This study showed high occurrence of broiler meat contamination with Campylobacter spp. at a retail level in Lithuania. Therefore the risk for consumers should be evaluated and an improvement of control measures at poultry production and retail level should be considered to reduce the risk for consumer’s infection with Campylobacter spp.
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- 2010
37. Mikrobiologinės taršos rizikos vertinimas paukštienoje. Campylobacter spp
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Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Milius, Jonas, Lithuanian Veterinary Academy, Maciukevičienė, Justė, Stanevičius, Zenonas, Petkevičius, Saulius, Lukoševičius, Leonardas, Zaborskienė, Gintarė, Sekmokienė, Dalia, Malakauskas, Mindaugas, Matusevičius, Algimantas, Vaitkevičienė, Milda, Milius, Jonas, Lithuanian Veterinary Academy, and Maciukevičienė, Justė
- Abstract
Darbo tikslas: 1. Ištirti ir įvertinti viščiukų broilerių pulkuose kampilobakterijų rūšių paplitimą. 2. Įvertinti riziką užsikrėsti kampilobakterioze žmonėms nuo viščiukų broilerių mėsos. Darbo uždaviniai: 1. Įvertinti Campylobacter spp. paplitimą viščiukų broilerių pulkuose. 2. Įvertinti Campylobacter spp. paplitimą viščiukų broilerių skerdenose. 3. Ištirti dažniausiai pasitaikančias Campylobacter rūšis viščiukų broilerių skerdimo partijose. 4. Išanalizuoti atliktų mikrobiologinių tyrimų svarbą. 5. Palyginti kampilobakterijų paplitimą tarp viščiukų broilerių Lietuvoje ir Europos Sąjungoje. 6. Įvertinti mikrobiologinės taršos riziką paukštienoje ir galimybę užsikrėsti kampilobakterioze. Išvados: 1. 45,4 % skerdžiamų viščiukų broilerių partijų buvo užsikrėtusios kampilobakterijomis; 2. 39,6 % skerdenų užterštos kampilobakterijomis; 3. Dažniausiai aptikta rūšis buvo Campylobacter jejuni, šiek tiek rečiau Campylobacter coli , ir mažai Campylobacter lari; 4. Padidėjus kampilobakterijų paplitimui viščiukų broilerių pulkuose ir skerdenose atitinkamai padažnėja žmonių kampilobakteriozės susirgimų atvejų skaičius vasaros sezonu; 5. Kampilobakterijomis užteršta viščiukų broilerių mėsa yra rizikos veiksnys, sukeliantys žmonių susirgimus kampilobakterioze., Master’s task purposis - to investigate Lithuania reared flocks of broiler and Campylobacter contamination of carcases and to assess the risk of microbial contamination as a major cause of human Campylobacteriosis. Literary analysis, an overview of disease, potential microbiological pollution sources, and the way of dissemination in broiler flocks and carcases. The incidence of Campylobacteriosis in Lithuania and EU. The study was mode in the slaughterhouse broiler chickens of the blind gut and carcass samples from the same batch slaughtering 6 of country. There are was found that in our country slaughtered chicken flocks are infected with 45.4% of Campylobacter, in am country and contaminated carcases – 39,6 %. Examination of contamination of carcases Identified species distribution rate - Campylobacter jejuni accounted for 80 % of all detected species, and Campylobacter coli accounted for 19 % of Campylobacter lari 1 % of all detected species. The broiler flocks for Campylobacter of infection Campylobacter jejuni is 81.3 % and Campylobacter coli was detected in 18.7 % of all species. Peak detection of campylobacter in broiler chickens and carcasses from flocks during the study month of September. Microbial contamination for broiler of campylobacter without hygiene and proper heat treatment can cause a risk of to get nutritional zoonoses - campylobacteriosis.
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- 2010
38. Echinokokozių epizootiniai duomenys. Lietuvos rudųjų lapių (vulpes vulpes) ir usūrinių šunų (nyctereutes procyonoides) helmintai
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Šarkūnas, Mindaugas, Petkevičius, Saulius, Špakauskas, Vytautas, Šalomskas, Algirdas, Barauskas, Giedrius, Kupčinskas, Limas, Malakauskas, Alvydas, Torgerson, Paul, Deplazes, Peter, Lithuanian Veterinary Academy, Bružinskaitė, Rasa, Šarkūnas, Mindaugas, Petkevičius, Saulius, Špakauskas, Vytautas, Šalomskas, Algirdas, Barauskas, Giedrius, Kupčinskas, Limas, Malakauskas, Alvydas, Torgerson, Paul, Deplazes, Peter, Lithuanian Veterinary Academy, and Bružinskaitė, Rasa
- Abstract
Disertacijoje aprašyti tyrimai Lietuvoje atlikti pirmą kartą. Tyrimais nustatytas E. multilocularis ir E. granulosus paplitimo Lietuvoje mastas ir pagrindiniai šių zoonotinių cestodų platintojai. Taip pat įvertintas žmonių alveolinės ir cistinės echinokokozių paplitimo mastas. Tyrimų rezultatai parodė, kad diagnozuojamų žmonių, sergančių echinokokoze daugėja, ir Lietuva yra naujai patvirtinta E. multilocularis endeminė zona Europoje. Pagrindinės E. multilocularis platintojos yra rudosios lapės, o E. granulosus – kaimo šunys. Atlikę molekulinius tyrimus nustatėme E. granulosus padermę ir E. granulosus endeminę teritoriją. Gauti rezultatai padės vykdant E. multilocularis ir E. granulosus kontrolę., Red foxes and raccoon dogs as well as domestic dogs are transmitters of few important zoonotic helminths such as E. multilocularis, E. granulosus, Trichinella spp. and Toxocara canis that can cause AE, CE, trichinellosis and toxocarosis respectively. Although a rare disease in humans, AE is of considerable public health importance because it can be lethal. Human CE is less pathogenic than AE however, is more widespread and in endemic areas annual incidemce rates for CE reach up to 8 cases per 100,000 inhabitants. Published data on both Echinococcus species is missing in Lithuania. No information is available on the prevalence and the risks for transmission of E. multilocularis in Lithuania – the most dangerous zoonotic helminth species. E. multilocularis is of great concern in many European countries including the neighboring countries Poland, Belarus and Estonia where these species have already been detected in red foxes. The last study on E. granulosus was performed in 1964 by Danilevičius E. Since then, the agriculture has changed significantly in Lithuania. Large industrial pig and cattle farms collapsed after the independence and new small farms started to build up. Since Lithuania became a member of EU pig farms started to enlarge. However, recently 36% of pigs are still reared in small family farms (Department of Statistics) were home slaughtering for local consumption of pork is traditional. Additionally, no information is available on E. granulosus strains in... [to full text]
- Published
- 2007
39. Efficacy against nematode infections and safety of afoxolaner plus milbemycin oxime chewable tablets in domestic dogs under field conditions in Europe.
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Dantas-Torres, Filipe, Rehbein, Steffen, Knaus, Martin, Dollhofer, Doris, Kley, Katrin, Visser, Martin, Lechner, Joerg, Mihalca, Andrei, Mirabito, Rosamaria, Petkevičius, Saulius, Rapti, Dhimitër, Shukullari, Enstela, Sedeilhan, Michel, Mallouk, Yasmina, Lebon, Wilfried, Jeannin, Philippe, Breiltgens, Tatjana, Brianti, Emanuele, Capári, Balázs, and Gau, Michel
- Subjects
INSECTICIDES ,VETERINARY drugs ,DOGS ,NEMATODES ,DRUG efficacy - Abstract
Afoxolaner (AFX) plus milbemycin oxime (MO) combination chewable tablets (NexGard Spectra®, Merial) were evaluated for safety and efficacy against naturally acquired nematode infections in domestic dogs in a multi-centre, positive control, blinded field study using a randomized block design based on the order of presentation for allocation. In total, 408 dogs confirmed positive for naturally acquired infections of intestinal nematodes by pre-treatment faecal examination were studied in ten countries in Europe (Albania, Austria, Bulgaria, France, Germany, Hungary, Italy, Lithuania, Romania and Slovakia). Pre-treatment faecal examination revealed Toxocara, Toxascaris, hookworm, Trichuris and/or Capillaria nematode infections in 134, 30, 223, 155 and 14 dogs, respectively. Dogs were allocated to one of two treatment groups in a ratio of 1, AFX + MO chewables (≥2.5 mg AFX + ≥0.5 mg MO per kg body weight, according to dose bands; 207 dogs), and 1, MO plus praziquantel (PRZ) chewables (Milbemax®, Novartis; ≥0.5 mg MO + ≥5 mg PRZ per kg body weight, according to the manufacturer's instructions; 201 dogs) and treated once. For evaluation of efficacy based on reduction of faecal nematode egg counts, two faecal samples, one collected prior to treatment and one collected 9 to 21 days after treatment, were examined using modified McMaster techniques. For evaluation of systemic safety, dogs were examined by a veterinarian before treatment administration and at study end, and dog owners observed the health status of their dogs until the end of the study and reported any abnormal observation. For dogs treated with AFX + MO chewables, the efficacy was 99.7, 99.7, 97.2, 99.7 and 99.7 % for Toxocara, Toxascaris, hookworm, Trichuris and Capillaria, respectively; and the efficacy was 99.5, 99.4, 94.3, 99.9 and 98.0 %, respectively, for the MO + PRZ-treated dogs ( p ≤ 0.002 for all nematodes and both treatments). For Toxocara, hookworm and Trichuris, non-inferiority analysis demonstrated that the efficacy of AFX + MO chewable tablets was equal to or better than that of MO + PRZ. In spite that both treatments were ≥98 % efficacious against Toxascaris and Capillaria, a hypothesis of non-inferiority for both genera could not be established due to the low number of dogs infected with these parasites. No treatment-related adverse experiences were observed throughout the study. For both treatments, all dogs were given a systemic safety score of 'excellent' apart from one dog in each treatment group which received a score of 'acceptable'. AFX + MO combination chewables were shown to be safe and demonstrated a high level of efficacy when administered once to dogs infected with a broad range of parasitic nematodes under field conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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40. Prevalence and epidemiological features of bovine viral diarrhoea virus infection in Lithuania
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Mockeliūnien≐, Violeta, primary, Šalomskas, Algirdas, additional, Mockeliūnas, Raimundas, additional, and Petkevičius, Saulius, additional
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- 2004
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41. The Influence of Host Diet and Fasting on Helminth Infection: review
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Petkevičius, Saulius, primary
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- 1998
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42. KIAULIŲ VIRŠKINAMOJO TRAKTO HELMINTOZIŲ PAPLITIMAS LIETUVOJE PRIKLAUSOMAI NUO GYVULIO AMŽIAUS IR FERMŲ DYDŽIO.
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Petkevičius, Saulius and Pereckienė, Asta
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HELMINTHS , *SWINE diseases , *FARM produce , *PIGLETS - Abstract
The prevalence of gastrointestinal helminths in Lithuania was studied in January 2007 - July 2008. Twenty large industrial farms (LIF) (4000-33000 pigs), 45 conventional farms (CF) (400-4000 pigs) and 26 back yard farms (BY) (1-10 pigs) were randomly selected for sampling. All farms had exclusively indoor facilities, as do almost all Lithuanian swine herds. In total 1422 faecal samples from pigs of 5 age groups (piglets, weaners, fatteners, gilts, sows/boars) were collected individualy and examined for helminth eggs. Faecal egg counts were carried out by a concentration McMaster technique (Henriksen & Aagaard, 1976) with a lower detection limit of 20 eggs per gram (EPG). In ILF, CF and BY infections with Ascaris suum were found in 6%, 7% and 14% of fatteners and in 10%, 14% and 28% of gilts, respectively. Infections with Oesophagostomum spp. were observed in ILF, CF and BY: in 6%, 8% and 14% of fatteners, in 18% and 22% of gilts (no data on BY) and in 12% and 19% of sows (no data on BY), respectively. Eggs of Trichuris suis were found in 1% and 5% of gilts in LIF and CF, and in 2% and 6% of sows in LIF and BY, respectively. The results of this study demonstrated that the main gastrointestinal helminths of pigs in Lithuania are A. suum and Oesophagostomum spp. and the most heavily infected age groups are the fatteners and the gilts. Pigs on back yard farms were more intensively infected compared to large industrial and conventional farms (P<0.05). Variables concerning anthelmintic strategy, feed and floor type, bedding, cleaning and disinfection deserve further study. [ABSTRACT FROM AUTHOR]
- Published
- 2009
43. MCMASTER METODO MODIFIKACIJŲ PALYGINIMAS DIAGNOZUOJANT AVIŲ TRICHOSTRONGILIOZĘ.
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Pereckienė, Asta, Petkevičius, Saulius, Šarkunas, Mindaugas, and Šalomskas, Algirdas
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SHEEP , *FECES , *MICROBIOLOGY , *FECES examination , *TRICHOSTRONGYLIDAE , *TRICHOSTRONGYLUS - Abstract
The comparative efficacies of seven published McMaster method modifications for faecal egg counting were evaluated on sheep faecal samples containing Trichostrongylus spp. eggs. In the present study compared methods were reported by: I - Henriksen and Aagaard, 1976; II - Kassai, 1999; III and IV - Urquhart et al., 1996 (centrifugation and non-centrifugation methods); V and VI - Grønvold, 1991 (salt solution, and salt and glucose solution); VII - Thienpont, 1986. Each method was evaluated after the examination of 30 samples of faeces. Comparisons were made as to the number of samples found to be positive by each of the methods, the total egg counts per gram (EPG) of faeces, the variations in EPG obtained in the samples examined, and the ease of use of each of the methods. The positive samples were identified by counting Trichostrongylus spp. eggs in one, two and three sections of newly designed McMaster chamber. Our study showed that the most complex was Method I, and the simplest and quickest analysis was performed by Method VII. Examination of all three chambers resulted in five methods (I-IV, and VI) having 100% sensitivity, while methods V and VII had 97.8% and 95,6% sensitivity, respectively. Mean egg counts in two chambers varied from 1082 EPG (Method II) to 422 EPG (Method IV). Based on the mean egg counts for two chambers, an efficiency coefficient was calculated and equated to 1 for the highest egg count (Method II) and lowest (0.39) for Method IV. Our results have shown that from seven evaluated methods Method I (Henriksen and Aagaard) was most stable and sensitive. Examing two or three sections of the McMaster chamber resulted in increased sensitivity for all methods. Efficiency coefficients make it possible not only to recalculate and unify results of faeces examination obtained by any method but also to interpret coproscopical examinations by other authors. [ABSTRACT FROM AUTHOR]
- Published
- 2007
44. THE INTERACTION BETWEEN INTESTINAL HELMINTH INFECTION AND HOST NUTRITION. REVIEW.
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Petkevičius, Saulius
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HELMINTHS , *INTESTINAL infections , *PARASITES , *GASTROINTESTINAL system , *NUTRITION disorders , *NITROGEN excretion , *TISSUES , *NUTRITION education - Abstract
The regulation of helminth populations in the host's gastrointestinal tract is a complex process, influenced by host immunological and nutritional status, age and breed of the animal. The interaction between helminth infection and nutrition can be considered from two interrelated points of view: the influence of the helminth infection on the host's physiology and nutrition and the effect of host nutrition on the helminth populations, i.e. their establishment, persistence and reproductive capacity. The first point of view has been the subject of numerous investigations over the past decade. It was estimated that common features of infection with intestinal helminths are: a reduction in voluntary feed intake, reduction of the digestibility's of dry and organic matter, a decrease in efficiency of feed utilization, significantly higher nitrogen output, and a rise in plasma urea concentration. It was described that only a limited amount of studies have examined the effects of nutrition on the parasite response in the parasite infected host, and even fewer have considered the events occurring at the intestinal level, where absorption of nutrients occurs, intestinal parasites reside, and the gastrointestinal associated tissues play role in directing both the local and the more systemic responses. In this review the reference is made to the important literature related to the effect of different nutrients, e. g. carbohydrate, protein, fat, non-organic constituens and malnutrition on the helminth populations in host. It appeared that gastrointestinal helminths have very specific physico-chemical requirements of their host gut environment, and nutritionally mediated changes have a direct influence on the parasite population. Furthermore, the mechanisms by which different nutrients influence helminth infection are addressed. [ABSTRACT FROM AUTHOR]
- Published
- 2007
45. McMASTER METODO MODIFIKACIJŲ EFEKTYVUMAS DIAGNOZUOJANT KIAULIŲ VIRŠKINAMOJO TRAKTO PARAZITUS.
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Pereckienė, Asta, Petkevičius, Saulius, and Šarkflnas, Mindaugas
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- *
COMPARATIVE studies , *FECES examination , *FECAL impaction , *SWINE , *GASTROINTESTINAL system , *TECHNICAL specifications - Abstract
The study was designed to compare the seven modifications of McMaster faecal egg counting (FEC) method to evaluate Ascaris suum eggs in pigs faeces. Thirty fresh faecal samples positive to A. suum for each modification were evaluated. The analysis was based on the number of positive samples, total number of A. suum eggs, variation of the total egg counts per gram (EPG) of faeces and the ease of use of each of the modifications. The following modifications were evaluated as reported by: (I) - Henriksen ir Aagaard (1976), (II) - Kassai (1999), (III) ir (IV) - Urquhart (1996), (V) ir (VI) - Granvold (1991) bei (VII) - Thienpont (1986). The highest EPG was obtained by method (I) modification. To this result was given the grade of the highest efficiency equaled to 1. The efficiency coefficient of each modification was calculated, which varied from 0,34 to 1. The lowest variation of egg counts were found by method (V). The results from this study demonstrated that from seven modifications method (VII) was the easest and quickest but least sensitive, and method (I) was most complex, but most sensitive. Our study demonstrated, that examining two or three chambers of the McMaster chamber resulted in increased sensivity for all methods. [ABSTRACT FROM AUTHOR]
- Published
- 2006
46. THE INFLUENCE OF NODULAR WORMS AND CARBOHYDRATES ON MORPHOLOGY AND PROLIFERATION OF EPITHELIAL CELLS IN THE LARGE INTESTINE OF PIGS.
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Petkevičius, Saulius and Knudsen, Knud Erik Bach
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SWINE infections , *HELMINTHS , *ESOPHAGUS diseases , *MUCINS , *CARBOHYDRATES , *LABORATORY swine , *LARGE intestine - Abstract
The influence of nodular worms Oesophagostomum dentatum infection and dietary carbohydrates on the morphology characteristics and epithelial cell proliferation in the large intestine of pigs was investigated. Thirty-two worm free weaners were randomly divided into four groups (A-D), of eight animals in each. Pigs in groups A (Control) and B (Infected) were fed Diet 1, and pigs in groups C (Control) and D (Infected) were fed Diet 2. The two diets were formulated: Diet 1 contained barley flour, oat husk meal plus soya bean meal (55% : 21%: 24%) and Diet 2 - barley flour, inulin and sugar beet fibre (SBF) (80.1% : 7%: 12.9%) plus soya bean meal (3:1). The two infected pig groups (B and D) were infected with 6000 infective larvae of O. dentatum and all 32 pigs were slaughtered 12 weeks p. i. The combination of O. dentatum infection and highly fermentable dietary carbohydrates affected the mucosal architecture, the epithelial cell proliferation and mucin secretion of the large intestine. These dietary carbohydrates in control pigs significantly influenced the tissue weight of caecum and colon. Infection with O. dentatum had significant effect on the gut wall architecture, because the changes in the affected gut sections corresponded directly to the number of worms present. [ABSTRACT FROM AUTHOR]
- Published
- 2005
47. Does the in vitroegg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus?
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Babják, Michal, Königová, Alžbeta, Urda Dolinská, Michaela, Kupčinskas, Tomas, Vadlejch, Jaroslav, von Samson-Himmelstjerna, Georg, Petkevičius, Saulius, Várady, Marián, Babják, Michal, Königová, Alžbeta, Urda Dolinská, Michaela, Kupčinskas, Tomas, Vadlejch, Jaroslav, von Samson-Himmelstjerna, Georg, Petkevičius, Saulius, and Várady, Marián
- Abstract
Considerable research has been directed towards optimising in vitrotests that can diagnose resistance in pre-parasitic stages of parasites. The objective of this study was to compare the in vivofaecal egg count reduction test (FECRT), the in vitroegg hatch test (EHT), and the molecular determination of the frequency of a codon 200 allele of β-tubulin isotype 1 associated with benzimidazole resistance in larval stages of Haemonchus contortusobtained from infected goats. Animals were infected with composite infective doses representing 10, 20, 30, 40, 60, and 80% resistant alleles. Faecal samples for the EHT were collected on 28, 33, and 35 days post-infection. The results of the in vivoFECRT indicated that albendazole treatment reduced infections consisting of composite doses of 10, 20, 30, 40, 60, and 80% larvae of the resistant isolate by 91.3, 78.0, 63.3, 48.4, 36.5, and 41.4%, respectively. The drug concentration at which 50% of the eggs were prevented from developing hatching larvae (ED50) in the in vitroEHT varied from 0.09 ± 0.01 to 15.63 ± 12.10 μg/mL thiabendazole. The results of the in vitroEHT indicated that the test could estimate in vivoresistance well. The EHT could thus accurately estimate the in vivoefficacy of the drug and percentage of the resistance allele in the population using hatching parameters in delineation doses. This finding was also supported by comparing the FECRT data to the hatching percentages in the EHT on 30 goat farms in Slovakia with natural mixed infections of gastrointestinal parasites.
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- 2021
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48. SKIRTINGŲ LAKTACIJŲ KARVIŲ, SERGANČIŲ ENDOMETRITU, GIMDOS BAKTERINIS UŽTERŠTUMAS.
- Author
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Šiugždaitė, Jūratė, Juodžentis, Vilmantas, and Petkevičius, Saulius
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BACTERIAL diseases in animals , *COW diseases , *UTERINE diseases , *ENDOMETRITIS , *LACTATION in cattle , *MICROBIOLOGY , *CLAVULANIC acid - Abstract
Bacteria play an important role in the pathogenesis of endometritis in cows. The aim of the present investigation was to determine the bacteria from the uteri of different lactation cows on endometritis. In total, forty seven secretion samples from the uteri of the 1st to 5th lactation cows were investigated. Samples for microbiological analysis were collected with sterile catheters from uterine cervix of dairy cows of different lactation. Bacteria were identified according to biochemical and antigenic properties. The Kirby Bauer method was used to determine susceptibility of the pathogenic isolated bacteria to antimicrobial substances. Bacteria were isolated in 39 (83.0 percent) cow's uterine secretion samples (from 47 investigated samples). The pure culture was determined in 18 (38.30 percent) samples, two types of bacteria (mixed culture) were identified in 16 (34.04 percent) samples, and three types in 5 (10.64 percent) samples. The results revealed that environmental bacteria were the most common isolates from cases of endometritis in the examined cows: Enterococcus faecalis (36.2 %), Streptococccus uberis (19.1 %) and Escherichia coli (44.7 %). Fourteen cows had retained placenta (29.79 %) in the first postpartum week. The main agent Escherichia coli was isolated in 92.8 % (p<0.01) of cows. Statistical analysis showed that isolation of Escherichia coli influenced the development of endometritis and the difference in lactation influenced the retention of placenta (p <0.03). Gram-positive and Gram-negative bacteria strains isolated from the uteri of different lactation cows on endometritis were most sensitive to amoxicillin with clavulanic acid. [ABSTRACT FROM AUTHOR]
- Published
- 2013
49. SEROPREVALENCE OF CANINE HERPES VIRUS IN LITHUANIAN DOG POPULATION.
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Musayeva, Kristina, Šengaut, Jakov, Petkevičius, Saulius, Malakauskas, Alvydas, Gerulis, Gediminas, and Šalomskas, Algirdas
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HERPESVIRUS diseases in animals , *SEROPREVALENCE , *DOG breeding , *VIRUS diseases in dogs , *ANIMAL health , *DOGS , *ENZYME-linked immunosorbent assay - Abstract
Canine herpesvirus type 1 (CHV-1) is presumed to be enzootic in dogs all over the world, but no information was available regarding the seroprevalence to CHV-1 from European north-east countries. The aim of the present study is to determine seroprevalence to CHV-1 in the Lithuanian canine population. Twenty dogs from one breeding kennel and seventy three dogs from veterinary hospital were tested for antibodies to CHV-1 by ELISA. Seropositive animals were identified both in kennel dogs and household dogs (85% and 11%, respectively). However, the number of seropositive individuals among the kennel dogs was seven times higher compared to the pet group (RR=7.3, CI 2.2-23.2, P<.001). The infection rate was significantly higher in oldest dogs group compared to young (P<.001) and medium age (P<0.05) dogs. The health status and sex had no significant influence on serological status of the dogs(P>0.05). [ABSTRACT FROM AUTHOR]
- Published
- 2013
50. INSEKTOAKARICIDŲ POVEIKIS ŠUNŲ IR KAČIŲ OTODEKTOZEI.
- Author
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Karpovaitė, Agneta, Špakauskas, Vytautas, Petkevičius, Saulius, and Daunoras, Gintaras
- Subjects
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TREATMENT of cat diseases , *VETERINARY therapeutics , *DOG diseases , *MITE infestations , *DISEASE prevalence , *DISSECTING microscopes , *FIPRONIL - Abstract
A total of 133 dogs and 133 cats were examined through direct smear of ear samples using a stereoscopic microscope, for the parasitological diagnostic. Mite infestation was observed in 18 dog and 40 cats with a prevalence of 12% and 30%. Eight cats with otacariasis were treated with a single spot-on application of selamectin (Stronghold®), and 32 cats were treated with ear drops Canaural®. Eight dogs with otacariasis were treated with a single spot-on application of fipronil (Frontline®) and eight dogs with Neostomosan®. The presence of parasites was assessed before treatment and at 7, 14 and 21 days after first treatment. The animals were also evaluated clinically at each assessment period. Most effective insectoacaricidic medication for cats was selamectin, as it showed the maximum result in destruction of O. cynotis ticks after 7 days of treatment and its efficacy did not change the entire study period (21 days). Against O. cynotis, Canaural® eliminated mites in 96.88% of cats by day 21. Frontline® and Neostomosan® solution resulted in a treatment success rate (cure rate) of 75% 21 days after treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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