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207 results on '"Petit, Valérie"'

6. UVB radiation suppresses Dicer expression through β-catenin.

Author

Aktary, Zackie, Petit, Valérie, Berlin, Irina, Raymond, Jeremy, Berger, Frederique, Charoenchon, Nisamanee, Sage, Evelyne, Bertrand, Juliette, and Larue, Lionel

Subjects
*GENE expression, *PHOSPHATIDYLINOSITOL 3-kinases, *BINDING sites, *MELANOCYTES, *CELLULAR signal transduction
Abstract

Ultraviolet (UV) rays prompt a natural response in epidermal cells, particularly within melanocytes. The changes in gene expression and related signaling pathways in melanocytes following exposure to UV radiation are still not entirely understood. Our findings reveal that UVB irradiation suppresses the expression of Dicer (also known as Dicer1). This repression is intricately linked to the activation of the phosphoinositide 3-kinase (PI3K), ribosomal S6 kinase (RSK) and Wnt-β-catenin signaling pathways, and is directly associated with transcriptional repression by β-catenin (also known as CTNNB1). Notably, we have identified specific binding sites for the TCF/LEF-β-catenin complex in the Dicer promoter. Collectively, these results emphasize the significance of the UV-induced pathway involving the TCF/LEF-β-catenin complex, which impacts Dicer expression. UV radiation also reduced the levels of specific microRNAs known to be important in the biology of melanocytes. This pathway holds potential importance in governing melanocyte physiology. [ABSTRACT FROM AUTHOR]

Published
2024
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7. A caveolin-dependent and PI3K/AKT-independent role of PTEN in β-catenin transcriptional activity.

Author

Conde-Perez, Alejandro, Gros, Gwendoline, Longvert, Christine, Pedersen, Malin, Petit, Valérie, Aktary, Zackie, Viros, Amaya, Gesbert, Franck, Delmas, Véronique, Rambow, Florian, Bastian, Boris C, Campbell, Andrew D, Colombo, Sophie, Puig, Isabel, Bellacosa, Alfonso, Sansom, Owen, Marais, Richard, Van Kempen, Leon CLT, and Larue, Lionel

Subjects
Cell Line, Tumor, Melanocytes, Animals, Mice, Transgenic, Humans, Mice, Melanoma, Skin Neoplasms, GTP Phosphohydrolases, Glycogen Synthase Kinase 3, Cadherins, Membrane Proteins, MicroRNAs, Microscopy, Fluorescence, Blotting, Western, Prognosis, Immunohistochemistry, Proto-Oncogene Proteins c-akt, PTEN Phosphohydrolase, beta Catenin, Caveolin 1, Transcriptional Activation, Feedback, Physiological, Phosphatidylinositol 3-Kinases, Glycogen Synthase Kinase 3 beta, Cell Line, Tumor, Transgenic, Microscopy, Fluorescence, Blotting, Western, Feedback, Physiological
Abstract

Loss of the tumour suppressor PTEN is frequent in human melanoma, results in MAPK activation, suppresses senescence and mediates metastatic behaviour. How PTEN loss mediates these effects is unknown. Here we show that loss of PTEN in epithelial and melanocytic cell lines induces the nuclear localization and transcriptional activation of β-catenin independent of the PI3K-AKT-GSK3β axis. The absence of PTEN leads to caveolin-1 (CAV1)-dependent β-catenin transcriptional modulation in vitro, cooperates with NRAS(Q61K) to initiate melanomagenesis in vivo and induces efficient metastasis formation associated with E-cadherin internalization. The CAV1-β-catenin axis is mediated by a feedback loop in which β-catenin represses transcription of miR-199a-5p and miR-203, which suppress the levels of CAV1 mRNA in melanoma cells. These data reveal a mechanism by which loss of PTEN increases CAV1-mediated dissociation of β-catenin from membranous E-cadherin, which may promote senescence bypass and metastasis.

Published
2015

8. BRN2 is a non-canonical melanoma tumor-suppressor

Author

Hamm, Michael, Sohier, Pierre, Petit, Valérie, Raymond, Jérémy H., Delmas, Véronique, Le Coz, Madeleine, Gesbert, Franck, Kenny, Colin, Aktary, Zackie, Pouteaux, Marie, Rambow, Florian, Sarasin, Alain, Charoenchon, Nisamanee, Bellacosa, Alfonso, Sanchez-del-Campo, Luis, Mosteo, Laura, Lauss, Martin, Meijer, Dies, Steingrimsson, Eirikur, Jönsson, Göran B., Cornell, Robert A., Davidson, Irwin, Goding, Colin R., and Larue, Lionel

Published
2021
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13. A shared perspective on in vitro and in vivo models to assay intestinal transepithelial transport of food compounds

Author

Hevia, Arancha, Ruas-Madiedo, Patricia, Faria, Miguel Angelo, Petit, Valérie, Alves, Bruna, Alvito, Paula, Arranz, Elena, Bastiaan-Net, Shanna, Corredig, Milena, Dijk, Wieneke, Dupont, Didier, Giblin, Linda, Graf, Brigitte Anna, Kondrashina, Alina, Ramos, Helena, Ruiz, Lorena, Santos-Hernández, Marta, Soriano-Romaní, Laura, Tomás-Cobos, Lidia, Vivanco-Maroto, Santiaga María, Recio, Isidra, Miralles, Beatriz, Hevia, Arancha, Ruas-Madiedo, Patricia, Faria, Miguel Angelo, Petit, Valérie, Alves, Bruna, Alvito, Paula, Arranz, Elena, Bastiaan-Net, Shanna, Corredig, Milena, Dijk, Wieneke, Dupont, Didier, Giblin, Linda, Graf, Brigitte Anna, Kondrashina, Alina, Ramos, Helena, Ruiz, Lorena, Santos-Hernández, Marta, Soriano-Romaní, Laura, Tomás-Cobos, Lidia, Vivanco-Maroto, Santiaga María, Recio, Isidra, and Miralles, Beatriz

Abstract

Assessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biological functionality of the food structures designed. This collaborative work aims to put into perspective the wide-range of models to assay the permeability of food compounds considering the particular nature of the different molecules, and, where possible, in vivo data are provided for comparison.

Published
2023

14. A shared perspective on in vitro and in vivo models to assay intestinal transepithelial transport of food compounds

Author

Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), Ministry of Agriculture, Nature and Food Quality (The Netherlands), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Hevia, Arancha, Ruas-Madiedo, Patricia, Faria, Miguel Angelo, Petit, Valérie, Alves, Bruna, Alvito, Paula, Arranz, Elena, Bastiaan-Net, Shanna, Corredig, Milena, Dijk, Wieneke, Dupont, Didier, Giblin, Linda, Graf, Brigitte Anna, Kondrashina, Alina, Ramos, Helena, Ruíz García, Lorena, Santos-Hernández, Marta, Soriano-Romaní, Laura, Tomás-Cobos, Lidia, Vivanco-Maroto, Santiaga María, Recio, Isidra, Miralles, Beatriz, Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal), Ministry of Agriculture, Nature and Food Quality (The Netherlands), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Hevia, Arancha, Ruas-Madiedo, Patricia, Faria, Miguel Angelo, Petit, Valérie, Alves, Bruna, Alvito, Paula, Arranz, Elena, Bastiaan-Net, Shanna, Corredig, Milena, Dijk, Wieneke, Dupont, Didier, Giblin, Linda, Graf, Brigitte Anna, Kondrashina, Alina, Ramos, Helena, Ruíz García, Lorena, Santos-Hernández, Marta, Soriano-Romaní, Laura, Tomás-Cobos, Lidia, Vivanco-Maroto, Santiaga María, Recio, Isidra, and Miralles, Beatriz

Abstract

Assessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biological functionality of the food structures designed. This collaborative work aims to put into perspective the wide-range of models to assay the permeability of food compounds considering the particular nature of the different molecules, and, where possible, in vivo data are provided for comparison.

Published
2023

15. Suppl Table S2 from TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, primary, Bizet, Martin, primary, Luciani, Flavie, primary, Calonne, Emilie, primary, Putmans, Pascale, primary, Nittner, David, primary, Singh, Nitesh Kumar, primary, Santagostino, Sara Francesca, primary, Petit, Valérie, primary, Larue, Lionel, primary, Marine, Jean Christophe, primary, and Fuks, François, primary

Published
2023
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16. Legend of supplementary data from TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, primary, Bizet, Martin, primary, Luciani, Flavie, primary, Calonne, Emilie, primary, Putmans, Pascale, primary, Nittner, David, primary, Singh, Nitesh Kumar, primary, Santagostino, Sara Francesca, primary, Petit, Valérie, primary, Larue, Lionel, primary, Marine, Jean Christophe, primary, and Fuks, François, primary

Published
2023
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17. Table S1 from TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, primary, Bizet, Martin, primary, Luciani, Flavie, primary, Calonne, Emilie, primary, Putmans, Pascale, primary, Nittner, David, primary, Singh, Nitesh Kumar, primary, Santagostino, Sara Francesca, primary, Petit, Valérie, primary, Larue, Lionel, primary, Marine, Jean Christophe, primary, and Fuks, François, primary

Published
2023
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18. Supplementary Figures from TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, primary, Bizet, Martin, primary, Luciani, Flavie, primary, Calonne, Emilie, primary, Putmans, Pascale, primary, Nittner, David, primary, Singh, Nitesh Kumar, primary, Santagostino, Sara Francesca, primary, Petit, Valérie, primary, Larue, Lionel, primary, Marine, Jean Christophe, primary, and Fuks, François, primary

Published
2023
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19. Data from TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, primary, Bizet, Martin, primary, Luciani, Flavie, primary, Calonne, Emilie, primary, Putmans, Pascale, primary, Nittner, David, primary, Singh, Nitesh Kumar, primary, Santagostino, Sara Francesca, primary, Petit, Valérie, primary, Larue, Lionel, primary, Marine, Jean Christophe, primary, and Fuks, François, primary

Published
2023
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22. The E-cadherin-ESR1-GRPR axis defines a sex-specific metastatic pathway in melanoma

Author

Raymond, Jérémy H., primary, Pouteaux, Marie, additional, Petit, Valérie, additional, Aktary, Zackie, additional, Luciani, Flavie, additional, Wehbe, Maria, additional, Gizzi, Patrick, additional, Bourban, Claire, additional, Martianov, Igor, additional, Davidson, Irwin, additional, Tomasetto, Catherine-Laure, additional, Betzer, Florence-Mahuteau, additional, Vergier, Béatrice, additional, Larue, Lionel, additional, and Delmas, Véronique, additional

Published
2022
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27. A Polymorphism in IRF4 Affects Human Pigmentation through a Tyrosinase-Dependent MITF/TFAP2A Pathway

Author

Praetorius, Christian, Grill, Christine, Stacey, Simon N., Metcalf, Alexander M., Gorkin, David U., Robinson, Kathleen C., Van Otterloo, Eric, Kim, Reuben S.Q., Bergsteinsdottir, Kristin, Ogmundsdottir, Margret H., Magnusdottir, Erna, Mishra, Pravin J., Davis, Sean R., Guo, Theresa, Zaidi, M. Raza, Helgason, Agnar S., Sigurdsson, Martin I., Meltzer, Paul S., Merlino, Glenn, Petit, Valerie, Larue, Lionel, Loftus, Stacie K., Adams, David R., Sobhiafshar, Ulduz, Emre, N.C. Tolga, Pavan, William J., Cornell, Robert, Smith, Aaron G., McCallion, Andrew S., Fisher, David E., Stefansson, Kari, Sturm, Richard A., and Steingrimsson, Eirikur

Published
2013
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32. Standardization of in vitro digestibility and DIAAS method based on the static INFOGEST protocol

Author

Egger, Lotti, Sousa, Raquel, Recio, Isidra, Abbühl, Lychou, Brügger, Cédric, Santos-Hernández, Marta, Macierzanka, Adam, Szumała, Patrycja, Rama, Addepalli, Mulet-Cabero, Ana-Isabel, Perez-Moral, Natalia, Brodkorb, Andre, Fitzpatrick, Conor, Rieder, Anne, Levi, Carmit Shani, Gonçales, Catarina, Rodriguez-Amado, Isabel, Nobre, Clarisse, Pinheiro Canton, Ana, Arranz, Elena, Bot, Francesca, A. O'Mahony, James, Grundy, Myriam M.-L., Pacheco-Cruz, Igor, Sepúlveda-González, Ailynne, Comi, Irene, Abrankó, László, Tormási, Judit, Gonzales, Felipe, Ruas-Madiedo, Patricia, Ruiz, Lorena, Faria, Miguel-Ângelo, Sobral, M.Madalena.C, Jiménez-Munoz, Luis Miguel, Corredig, Milena, Jimenez, Luis, Molly, Muleya, Rosa-Sibakov, Natalia, Ménard, Olivia, Orlien, Vibeke, Gousetti, Ourania, Lykkepetersen, Iben, Ferranti, Pasquale, Olias, Raquel, Delgado Andrade, Cristina, Assunção, Ricardo, López-Nicolás, Rubén, Simsek, Sebnem, Karakaya, Sibel, El, Sedef Nehir, Rezzi, Serge, Canarelli, Stéphane, Petit, Valérie, Emre Tuncil, Yunus, Hotrum, Nathalie, Broersen, Kerensa, Heimo, Dominique, Dubois, Sebastien, Portmann, Reto, and Giboulot, Anne

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, In vitro, food martrice, Digestion, Ethic, Amino acid assimilation
Abstract

The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, based on the validated static INFOGEST protocol, was developed and compared towards in vivo data. In parallel to the validation with in vivo data, the repeatability and reproducibility of the in vitro protocol were tested in an international ring trial (RT) with the aim to establish an international ISO standard method within the International Dairy Federation (IDF). Five different dairy products (skim milk powder, whole milk powder, whey protein isolate, yoghurt, and cheese) were analyzed in 32 different laboratories from 18 different countries, across 4 continents. Results: in vitro protein digestibilities based on Nitrogen, free R-NH2, and total amino acids as well as DIAAS values were calculated and compared to in vivo data, where available. Conclusion: The in vitro method is suited for quantification of digestibility and will be further implemented to other food matrices.

Published
2022

35. Efficacy of Targeted Radionuclide Therapy Using [131I]ICF01012 in 3D Pigmented BRAF- and NRAS-Mutant Melanoma Models and In Vivo NRAS-Mutated Melanoma

Author

Akil, Hussein, Quintana, Mercedes, Raymond, Jérémy H., Billoux, Tommy, Benboubker, Valentin, Besse, Sophie, Auzeloux, Philippe, Delmas, Véronique, Petit, Valérie, Larue, Lionel, D’Incan, Michel, Degoul, Françoise, and Rouanet, Jacques

Subjects
melanoma spheroid model, BRAF mutation, NRAS mutation, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, targeted radionuclide therapy, MEK inhibitors, lcsh:RC254-282
Abstract

Purpose: To assess the efficiency of targeted radionuclide therapy (TRT), alone or in combination with MEK inhibitors (MEKi), in melanomas harboring constitutive MAPK/ERK activation responsible for tumor radioresistance. Methods: For TRT, we used a melanin radiotracer ([131I]ICF01012) currently in phase 1 clinical trial (NCT03784625). TRT alone or combined with MEKi was evaluated in three-dimensional melanoma spheroid models of human BRAFV600E SK-MEL-3, murine NRASQ61K 1007, and WT B16F10 melanomas. TRT in vivo biodistribution, dosimetry, efficiency, and molecular mechanisms were studied using the C57BL/6J-NRASQ61K 1007 syngeneic model. Results: TRT cooperated with MEKi to increase apoptosis in both BRAF- and NRAS-mutant spheroids. NRASQ61K spheroids were highly radiosensitive towards [131I]ICF01012-TRT. In mice bearing NRASQ61K 1007 melanoma, [131I]ICF01012 induced a significant extended survival (92 vs. 44 days, p &lt, 0.0001), associated with a 93-Gy tumor deposit, and reduced lymph-node metastases. Comparative transcriptomic analyses confirmed a decrease in mitosis, proliferation, and metastasis signatures in TRT-treated vs. control tumors and suggest that TRT acts through an increase in oxidation and inflammation and P53 activation. Conclusion: Our data suggest that [131I]ICF01012-TRT and MEKi combination could be of benefit for advanced pigmented BRAF-mutant melanoma care and that [131I]ICF01012 alone could constitute a new potential NRAS-mutant melanoma treatment.

Published
2021

38. BRN2 is a non-canonical melanoma tumor-suppressor

Author

Hamm, Michael, primary, Sohier, Pierre, additional, Petit, Valérie, additional, Raymond, Jérémy H, additional, Delmas, Véronique, additional, Le Coz, Madeleine, additional, Gesbert, Franck, additional, Kenny, Colin, additional, Aktary, Zackie, additional, Pouteaux, Marie, additional, Rambow, Florian, additional, Sarasin, Alain, additional, Bellacosa, Alfonso, additional, Sanchez-del-Campo, Luis, additional, Mosteo, Laura, additional, Lauss, Martin, additional, Meijer, Dies, additional, Steingrimsson, Eirikur, additional, Jönsson, Göran B, additional, Cornell, Robert A, additional, Davidson, Irwin, additional, Goding, Colin R, additional, and Larue, Lionel, additional

Published
2021
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45. CLEC12B a new gene implicated in melanoma

Author

Montaudié, Henri, primary, Sormani, Sormani, primary, Dadone-Montaudié, Dadone-Montaudié, primary, E. BERANGER, Guillaume, primary, cheli, cheli, primary, Petit, Valérie, primary, Rocchi, Rocchi, primary, Franck, Gesbert, primary, LARUE, Lionel, primary, Passeron, Passeron, primary, and Tulic, Meri, primary

Published
2019
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47. TET2-dependent hydroxymethylome plasticity reduces melanoma initiation and progression

Author

Bonvin, Elise, Radaelli, Enrico, Bizet, Martin, Luciani, Flavie, Calonne, Emilie, Putmans, Pascale, Nittner, David, Singh, Nitesh Kumar, Santagostino, Sara Francesca, Petit, Valérie, Larue, Lionel, Marine, Jean-Christophe, Fuks, François, Bonvin, Elise, Radaelli, Enrico, Bizet, Martin, Luciani, Flavie, Calonne, Emilie, Putmans, Pascale, Nittner, David, Singh, Nitesh Kumar, Santagostino, Sara Francesca, Petit, Valérie, Larue, Lionel, Marine, Jean-Christophe, and Fuks, François

Abstract

Although numerous epigenetic aberrancies accumulate in melanoma, their contribution to initiation and progression remain unclear. The epigenetic mark 5-hydroxymethylcyto-sine (5hmC), generated through TET-mediated DNA modification, is now referred to as the sixth base of DNA and has recently been reported as a potential biomarker for multiple types of cancer. Loss of 5hmC is an epigenetic hallmark of melanoma, but whether a decrease in 5hmc levels contributes directly to pathogenesis or whether it merely results from disease progression-associated epigenetic remodeling remains to be established. Here, we show that NRAS-driven melanomagenesis in mice is accompanied by an overall decrease in 5hmC and specific 5hmC gains in selected gene bodies. Strikingly, genetic ablation of Tet2 in mice cooperated with oncogenic NRASQ61K to promote melanoma initiation while suppressing specific gains in 5hmC. We conclude that TET2 acts as a barrier to melanoma initiation and progression, partly by promoting 5hmC gains in specific gene bodies. Significance: This work emphasizes the importance of epigenome plasticity in cancer development and highlights the involvement of druggable epigenetic factors in cancer., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2019

48. CLEC12BDecreases Melanoma Proliferation by Repressing Signal Transducer and Activator of Transcription 3

Author

Montaudié, Henri, Sormani, Laura, Dadone-Montaudié, Bérengère, Heim, Marjorie, Cardot-Leccia, Nathalie, Tulic, Meri K., Beranger, Guillaume, Gay, Anne-Sophie, Debayle, Delphine, Cheli, Yann, Raymond, Jérémy H., Sohier, Pierre, Petit, Valérie, Rocchi, Stéphane, Gesbert, Franck, Larue, Lionel, and Passeron, Thierry

Abstract

The potential role of CLEC12B, a gene predominantly expressed by skin melanocytes discovered through transcriptomic analysis, in melanoma is unknown. In this study, we show that CLEC12B expression is lower in melanoma and melanoma metastases than in melanocytes and benign melanocytic lesions and that its decrease correlates with poor prognosis. We further show that CLEC12B recruits SHP2 phosphatase through its immunoreceptor tyrosine-based inhibition motif domain, inactivates signal transducer and activator of transcription 1/3/5, increases p53/p21/p27 expression/activity, and modulates melanoma cell proliferation. The growth of human melanoma cells overexpressing CLEC12B in nude mice after subcutaneous injection is significantly decreased compared with that in the vehicle control group and is associated with decreased signal transducer and activator of transcription 3 phosphorylation and increased p53 levels in the tumors. Reducing the level of CLEC12B had the opposite effect. We show that CLEC12Brepresses the activation of the signal transducer and activator of transcription pathway and negatively regulates the cell cycle, providing a proliferative asset to melanoma cells.

Published
2022
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50. TET2-Dependent Hydroxymethylome Plasticity Reduces Melanoma Initiation and Progression

Author

Bonvin, Elise, primary, Radaelli, Enrico, additional, Bizet, Martin, additional, Luciani, Flavie, additional, Calonne, Emilie, additional, Putmans, Pascale, additional, Nittner, David, additional, Singh, Nitesh Kumar, additional, Santagostino, Sara Francesca, additional, Petit, Valérie, additional, Larue, Lionel, additional, Marine, Jean Christophe, additional, and Fuks, François, additional

Published
2019
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