1. A novel fast-response and highly selective AIEgen fluorescent probe for visualizing peroxynitrite in living cells, C. elegans and inflammatory mice.
- Author
-
Ye YX, Chen XY, Yu YW, Zhang Q, Wei XW, Wang ZC, Wang BZ, Jiao QC, and Zhu HL
- Subjects
- Animals, Caenorhabditis elegans, Fluorescence, HeLa Cells, Humans, Mice, Fluorescent Dyes toxicity, Peroxynitrous Acid toxicity
- Abstract
Most of the ONOO
- fluorescent probes have restricted applications because of their aggregation-caused quenching (ACQ) effect, long response time and low fluorescence enhancement. Herein, we developed a novel AIEgen fluorescent probe (PE-XY) based on a benzothiazole and quinolin scaffold with high sensitivity and selectivity for imaging of ONOO- . The results indicated that probe PE-XY exhibited fast response towards ONOO- with 2000-fold enhancement of fluorescence intensity ratio in vitro . Moreover, PE-XY exhibited a relatively high sensitivity (limit of detection: 8.58 nM), rapid response (<50 s), high fluorescence quantum yield ( δ = 0.81) and excellent selectivity over other analytes towards ONOO- in vitro . Furthermore, PE-XY was successfully applied to detect endogenous ONOO- levels in living HeLa cells, C. elegans and inflammatory mice with low cytotoxicity. Overall, this work provided a novel fast-response and highly selective AIEgen fluorescent probe for real-time monitoring ONOO- fluctuations in living systems.- Published
- 2021
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