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A two-photon excited near-infrared fluorescent probe for imaging peroxynitrite during drug-induced hepatotoxicity and its remediation.
- Source :
-
Talanta [Talanta] 2021 Jan 01; Vol. 221, pp. 121607. Date of Electronic Publication: 2020 Sep 03. - Publication Year :
- 2021
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Abstract
- Drug-induced liver injury (DILI) has been a hot issue of public health, owing to its unpredictability and serious harm to public health. Peroxynitrite (ONOO <superscript>-</superscript> ) is an important biomarker for the assessment and diagnosis of DILI. In this article, based on a kind of rhodamine analogue with a near-infrared (NIR) emission (610 nm-800 nm) and a two-photon absorption cross section (54 GM), a two-photon excited NIR fluorescence probe (NIR-ONOO) for ONOO <superscript>-</superscript> was developed. With a high selectivity and a high sensitivity to ONOO <superscript>-</superscript> , NIR-ONOO has a linear range for detection of ONOO <superscript>-</superscript> from 5.0 × 10 <superscript>-8</superscript> to 1.0 × 10 <superscript>-5</superscript>  M, a good detection limit (15 nM) and a large fluorescence enhancement (340-fold). In addition, NIR-ONOO has been used to monitor ONOO <superscript>-</superscript> in cells with satisfactory results. Because of its two-photon excied NIR emission, NIR-ONOO also showed excellent performances for imaging ONOO <superscript>-</superscript> including low autofluorescence, stable and persistent fluorescence, and a deep penetration (204 μm). Finally, NIR-ONOO was successfully employed to image ONOO <superscript>-</superscript> in inflammatory mouse, drug-induced hepatotoxicity in cells and its remediation. All the results indicated that NIR-ONOO is a powerful chemical tool to image ONOO <superscript>-</superscript> and assay drug-induced hepatotoxicity.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 221
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 33076137
- Full Text :
- https://doi.org/10.1016/j.talanta.2020.121607