26 results on '"Pengju Cai"'
Search Results
2. Volatile components, total phenolic compounds, and antioxidant capacities of worm-infected Gomphidius rutilus
- Author
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Libin Sun, Wei He, Guang Xin, Pengju Cai, Yin Zhang, Zhiyong Zhang, Yunyun Wei, Bingxin Sun, and Xiaowen Wen
- Subjects
Nutrition. Foods and food supply ,TX341-641 - Abstract
This study evaluated the effects of worm infection on the volatile components, total phenolic compounds, and antioxidant capacities of Gomphidius rutilus. G. rutilus without worms (GW), G. rutilus infected by a small amount of worms (GS; infected area 50%) were investigated. The volatile components of G. rutilus were analyzed by simultaneous distillation–extraction (SDE) and headspace solid-phase microextraction (HS-SPME) using gas chromatography–mass spectrometry (GC–MS). A total of 17 and 19 types of volatile compounds were detected, including ketones, alcohols, benzene, alkenes, aldehydes, esters, acids, and alkanes. Alcohols comprised the most abundant compound in GL, GS, and GW. The relative content of 1-octen-3-ol was the highest in all mushrooms. The concentration of eight-carbon (C8) compounds relative to the total volatile compounds varied widely, ranging from 40% (GW) to 64.34% (GS) and 84.42% (GS) and to 91.59% (GL), respectively, among the three samples. The antioxidant capability and the total phenolic contents of G. rutilus were evaluated in this study. The highest total phenolic content (TPC) of 192.23 mg GAE/g was found in GL, which differed significantly (P < 0.05) from the latter two samples, whereas the lowest value of 156.11 mg GAE/g was found in GW. ABTS radical cation scavenging activity, FRAP ferric reducing antioxidant capacity (FRAP) radical scavenging activity, and oxygen radical absorbance capacity (ORAC) were investigated to screen the antioxidant properties of extracts. The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations (P < 0.01). Among the three types of samples, the phenolic compounds of GL exhibited the highest antioxidant capacity, showing the values of 0.089 mM TE/g for ABTS, 0.949 mM Fe2+ E/g for FRAP, and 1.952 M TE/g for ORAC. However, regarding the total antioxidant capacity, GS exhibited the highest antioxidant capacity, showing the values of 0.002648 mM TE/g for ABTS, 0.004437 mM Fe2+ E/g for FRAP, and 0.256 μM TE/g for ORAC. In conclusion, HS-SPME was more suitable for the extraction of volatile aroma components from G. rutilus. GL had the most abundant aroma components. GL had the highest TPC and antioxidant capacity compared with those of GS and GW, whereas GS showed the opposite results. Interestingly, GS was found to have the highest total antioxidant capacity in vitro. Based on these measured indicators, worm infection had no negative effect on the quality of G. rutilus. Therefore, worm-infected G. rutilus can also be consumed by humans. Keywords: Gomphidius rutilus, Worm infection, Volatile components, Total phenolic, Antioxidant capacity
- Published
- 2018
- Full Text
- View/download PDF
3. Au Clusters Treat Rheumatoid Arthritis with Uniquely Reversing Cartilage/Bone Destruction
- Author
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Fuping Gao, Qing Yuan, Pengju Cai, Liang Gao, Lina Zhao, Meiqing Liu, Yawen Yao, Zhifang Chai, and Xueyun Gao
- Subjects
Au clusters ,bone destruction ,inflammation ,rheumatoid arthritis ,Science - Abstract
Abstract Super‐small nanoclusters may intrinsically trigger specific molecular pathway for disease treatment in vitro/vivo. To prove the hypothesis the super‐small nanoclusters, e.g., Au clusters, are directly used to treat rheumatoid arthritis (RA) in vitro/vivo. RA is a chronic autoimmune disease that is characterized by the inflammation of joints and the unreversible destruction of the cartilage/bone. Au clusters significantly suppress lipopolysaccharide (LPS)‐induced proinflammatory mediator production in the murine macrophage cell line by inhibiting the signaling pathways that regulate the major proinflammatory mediator genes. In preclinical rat RA studies, Au clusters strongly prevent type II collagen‐induced rat RA without systemic side effects. Compared with the clinical first‐line anchored anti‐RA drug, methotrexate, Au clusters equally inhibit inflammation in vivo. Type II collagen‐induced rat RA is characterized with the destruction of cartilage/bone; treatment with Au clusters reverses the destruction of cartilage/bone to its normal state. This is because Au clusters directly inhibit receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclast differentiation and function through the downregulation of osteoclast‐specific genetic marker expression. However the methotrexate almost has no positive effect for this key issue in rat RA therapy. These data prove that the super‐small nanoclusters, e.g., Au clusters, could be a novel candidate nanodrug for RA treatment.
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- 2019
- Full Text
- View/download PDF
4. Peptide-Templated Gold Clusters as Enzyme-Like Catalyst Boost Intracellular Oxidative Pressure and Induce Tumor-Specific Cell Apoptosis
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Ya Zhang, Xiangchun Zhang, Qing Yuan, Wenchao Niu, Chunyu Zhang, Jiaojiao Li, Zhesheng He, Yuhua Tang, Xiaojun Ren, Zhichao Zhang, Pengju Cai, Liang Gao, and Xueyun Gao
- Subjects
gold clusters ,biocatalysis ,reactive oxygen species ,oxidative pressure-type tumor ,Chemistry ,QD1-999 - Abstract
Anticancer metallodrugs that aim to physiological characters unique to tumor microenvironment are expected to combat drug tolerance and side-effects. Recently, owing to the fact that reactive oxygen species’ is closely related to the development of tumors, people are committed to developing metallodrugs with the capacity of improving the level of reactive oxygen species level toinduce oxidative stress in cancer cells. Herein, we demonstrated that peptide templated gold clusters with atomic precision preferably catalyze the transformation of hydrogen peroxide into superoxide anion in oxidative pressure-type tumor cells. Firstly, we successfully constructed gold clusters by rationally designing peptide sequences which targets integrin ανβ3 overexpressed on glioblastoma cells. The superoxide anion, radical derived from hydrogen peroxide and catalyzed by gold clusters, was confirmed in vitro under pseudo-physiological conditions. Then, kinetic parameters were evaluated to verify the catalytic properties of gold clusters. Furthermore, these peptide decorated clusters can serve as special enzyme-like catalyst to convert endogenous hydrogen peroxide into superoxide anion, elevated intracellular reactive oxygen species levels, lower mitochondrial membrane potential, damage biomacromolecules, and trigger tumor cell apoptosis consequently.
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- 2018
- Full Text
- View/download PDF
5. Inhibitory effects of polyphenols from black chokeberry on advanced glycation end-products (AGEs) formation
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Wanbin Zhao, Pengju Cai, Nan Zhang, Tongtong Wu, Aidong Sun, and Guoliang Jia
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Glycation End Products, Advanced ,Glycosylation ,Phenols ,Polyphenols ,General Medicine ,Antioxidants ,Food Science ,Analytical Chemistry - Abstract
Plant-based polyphenols are known to exert mitigating effects on the harmful consequences of advanced glycation. In this study, the antioxidant and antiglycation properties of purified black chokeberry polyphenol and its dominant monomers were studied. The phenolics of black chokeberry had a significant inhibitory effect on glycation products at all stages. The highest inhibition of fructosamine (72.27%) was achieved by chlorogenic acid (CA). Epigallocatechin gallate (EGCG) showed an 84.47% inhibition of α-dicarbonyl and 54.44% inhibition of AGEs (advanced glycation end-products). However, the inhibition of α-dicarbonyl was impacted by the presence of Cu
- Published
- 2022
6. Inherently PET/CT Dual Modality Imaging Lipid Nanocapsules for Early Detection of Orthotopic Lung Tumors
- Author
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Xiangchun Zhang, Pengju Cai, Zhibo Liu, Chunyu Zhang, Liang Gao, Liu Yu, Dongdong Su, Xueyun Gao, Wenjiang Yang, Fuping Gao, Hui Liu, Huaidong Jiang, and Zhesheng He
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medicine.medical_specialty ,PET-CT ,Lung ,business.industry ,Lipid nanocapsules ,Biochemistry (medical) ,Biomedical Engineering ,Cancer ,Early detection ,General Chemistry ,Pet imaging ,medicine.disease ,Biomaterials ,medicine.anatomical_structure ,medicine ,Dual modality ,Radiology ,Stage (cooking) ,business - Abstract
Accurate diagnosis of cancer at an early stage is the key to reduce cancer mortality and improve survival. PET imaging has high sensitivity but low spatial resolution, while CT imaging has good spatial location information. Therefore, the combination of PET and CT imaging can provide complementary advantages to achieve accurate early diagnosis of tumors. However, currently developed PET or CT imaging agents have only a single function. Here, we designed and constructed a self-assembled lipid nanocapsule encapsulated with iodixanol and labeled with self-chelated
- Published
- 2022
7. Iodine-124 Labeled Gold Nanoclusters for Positron Emission Tomography Imaging in Lung Cancer Model
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Yu Liu, Fuping Gao, Sijia Wang, Han Weiwei, Xue Jingquan, Xueyun Gao, Wenjiang Yang, Pengju Cai, and Jianjun Wang
- Subjects
Fluorescence-lifetime imaging microscopy ,Lung Neoplasms ,Materials science ,Biomedical Engineering ,chemistry.chemical_element ,Bioengineering ,Peptide ,02 engineering and technology ,Iodine ,Nanoclusters ,Iodine Radioisotopes ,Cell Line, Tumor ,medicine ,Animals ,General Materials Science ,Lung cancer ,Lung ,chemistry.chemical_classification ,medicine.diagnostic_test ,Radiochemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease ,Human serum albumin ,Rats ,medicine.anatomical_structure ,chemistry ,Positron emission tomography ,Positron-Emission Tomography ,Gold ,0210 nano-technology ,medicine.drug - Abstract
This work reports the synthesis, radiolabeling and imaging studies of iodine-124 labeled peptide modified gold nanoclusters (AuNCs) as positron emission tomography (PET) tracer for lung cancer. The novel modified Au nanoclusters were successfully synthesized by conjugation of tumortargeting peptide luteinizing hormone releasing hormone (LHRH) to human serum albumin (HAS) as a scaffold, resulting in 73% labeling yield of 124I-LHRH-HSA AuNCs. After rapid purification, the radiochemical purity was above 98%. Dynamic PET study in normal rats showed high liver accumulation and rapid lung clearance. Both the PET and fluorescence imaging in A549 xenografted tumor model demonstrated certain amount of tumor uptake. In orthotopic lung cancer model, the tumor sites could be clearly visualized between 2 to 5 hours in PET images. The higher radioactivity concentration in the left lung which inoculated orthotopic tumor than right lung also exhibited the targeting properties. The biological properties of this iodine-124 labeled nanoclusters afford potential applications for early diagnosis of lung cancer with PET.
- Published
- 2020
8. A chlorin-lipid nanovesicle nucleus drug for amplified therapeutic effects of lung cancer by internal radiotherapy combined with the Cerenkov radiation-induced photodynamic therapy
- Author
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Wenjiang Yang, Liang Gao, Zhiyong Zhang, Pengju Cai, Fuping Gao, Xueyun Gao, Zhesheng He, Wencong Zhao, Huiju Jia, and Huangwei Wang
- Subjects
medicine.medical_specialty ,Lung Neoplasms ,Porphyrins ,medicine.medical_treatment ,Biomedical Engineering ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Mice ,chemistry.chemical_compound ,In vivo ,Cell Line, Tumor ,Internal medicine ,White blood cell ,medicine ,Animals ,General Materials Science ,Lung cancer ,Photosensitizing Agents ,Hematology ,Chemistry ,Therapeutic effect ,021001 nanoscience & nanotechnology ,medicine.disease ,Lipids ,0104 chemical sciences ,Radiation therapy ,medicine.anatomical_structure ,Pharmaceutical Preparations ,Photochemotherapy ,Chlorin ,Cancer research ,0210 nano-technology - Abstract
Traditional photodynamic therapy (PDT) requires external light excitation to produce reactive oxygen species (ROSs) for the treatment of tumors. Due to problems of light penetration, traditional PDT is limited by the location and depth of the tumor. In this study, we rationally designed and constructed a novel strategy to amplify the therapeutic effect of PDT. We prepared a chlorin-lipid nanovesicle based on the conjugates of chlorin e6 (Ce 6) and phospholipids, with the surface conjugating the aptamer for lung cancer targeting, GLT21.T. 131I-labeled bovine serum albumin (131I-BSA) was loaded into the chlorin-lipid nanovesicle cavity (131I-BSA@LCN-Apt). 131I not only plays a role in radiotherapy, but its Cerenkov radiation (CR), as an internal light source, can also stimulate Ce6 to produce ROSs without external light excitation. The in vitro and in vivo therapeutic effects in subcutaneous lung tumor models and orthotopic lung tumor models indicated that 131I-BSA@LCN-Apt produced a powerful anti-tumor effect through synergistic radiotherapy and CR-PDT, which almost caused complete tumor growth regression. After treatment, the survival time of the mice was significantly prolonged. During the treatment, no obvious side effects were found by histopathology of important organs, hematology and biochemistry analysis except the decrease of the white blood cell count (WBC). The study provides a major tool for deep-seated tumors to obtain amplified therapeutic effects by synergistic radiotherapy and CR-PDT without the use of any external light source.
- Published
- 2020
9. Comparison of the Therapeutic Effects of Gold Nanoclusters and Gold Nanoparticles on Rheumatoid Arthritis
- Author
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Ruoping Wang, Xiangchun Zhang, Pengju Cai, Fuping Gao, Jinsong Zhang, Xueyun Gao, Zhesheng He, Qing Yuan, and Yao Zhao
- Subjects
0206 medical engineering ,Biomedical Engineering ,Serum albumin ,Metal Nanoparticles ,Pharmaceutical Science ,Medicine (miscellaneous) ,Arthritis ,Bioengineering ,02 engineering and technology ,Proinflammatory cytokine ,Arthritis, Rheumatoid ,Mice ,chemistry.chemical_compound ,In vivo ,medicine ,Animals ,Nanotechnology ,General Materials Science ,Particle Size ,Bovine serum albumin ,biology ,Chemistry ,Serum Albumin, Bovine ,Glutathione ,021001 nanoscience & nanotechnology ,medicine.disease ,020601 biomedical engineering ,Molecular biology ,In vitro ,Rats ,Colloidal gold ,biology.protein ,Gold ,0210 nano-technology - Abstract
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and progressive cartilage and bone damage. In our previous studies, we found that Au clusters using glutathione as a template (GACs) produced profound anti-inflammatory effects in vitro on lipopolysaccharide (LPS)-induced inflammation in mouse macrophage RAW 264.7 cells and type II collagen-induced rat RA in vivo. In this study, we examined whether the template for Au clusters synthesis has an effect on its anti-inflammatory effect and whether Au nanoparticles with larger particle diameter produce the same anti-inflammatory effect. We synthesized Au clusters with bovine serum albumin (BSA) as a template (BACs), Au clusters with glutathione (GSH) as a template (GACs), and Au nanoparticles with glutathione as a template (GANs) and compared their anti-inflammatory effects in vitro and in vivo. These three Au nanomaterials can inhibit the production of lipopolysaccharide (LPS)-induced proinflammatory mediators and ameliorate type II collagen-induced rat RA. However, although the three Au nanomaterials produced similar anti-inflammatory effects, the GANs with larger particle sizes were less stable in vivo and accumulated in the peritoneum after intraperitoneal injection, resulting in poor absorption in vivo. The BACs showed relatively high liver accumulation due to the larger molecular weight of the outer shell. Therefore, we believe that the GACs are potential reliable nanodrugs for the treatment of RA.
- Published
- 2019
10. Gold Clusters Prevent Inflammation-Induced Bone Erosion through Inhibiting the Activation of NF-κB Pathway
- Author
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Kaixiao Hou, Xiangchun Zhang, Pengju Cai, Qing Yuan, Yawen Yao, Fuping Gao, Xueyun Gao, Jinling Yuan, Liang Gao, and Xiaojun Ren
- Subjects
Male ,Medicine (miscellaneous) ,Osteoclasts ,Inflammation ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,gold cluster ,Proinflammatory cytokine ,Cell Line ,chemistry.chemical_compound ,Mice ,Osteoclast ,In vivo ,Osteogenesis ,medicine ,Animals ,inflammatory bone destruction ,Bone Resorption ,Receptor ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,osteoclastogenesis ,Periodontitis ,NF-κB pathway ,biology ,Chemistry ,Macrophages ,RANK Ligand ,NF-kappa B ,NF-κB ,Cell Differentiation ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,medicine.anatomical_structure ,RAW 264.7 Cells ,RANKL ,biology.protein ,Cancer research ,Gold ,medicine.symptom ,0210 nano-technology ,Research Paper ,Signal Transduction - Abstract
Inflammation-induced bone erosion is a major pathological factor in several chronic inflammatory diseases that often cause severe outcomes, such as rheumatoid arthritis and periodontitis. Plenty of evidences indicated that the inflammatory bone destruction was attributed to an increase in the number of bone-resorbing osteoclasts. However, anti-resorptive therapy alone failed to prevent bone loss in an inflammatory condition. Conventional anti-inflammation treatments are usually intended to suppress inflammation only, but ignore debilitating the subsequent bone destruction. Therefore, inhibition of proinflammatory activation of osteoclastogenesis could be an important strategy for the development of drugs aimed at preventing inflammatory bone destruction. Methods: In this study, we synthesized a peptide coated gold cluster to evaluate its effects on inflammatory osteoclastogenesis in vitro and inflammation-induced bone destruction in vivo. The in vitro anti-inflammation and anti-osteoclastogenesis effects of the cluster were evaluated in LPS-stimulated and receptor activator of nuclear factor κB ligand (RANKL) stimulated macrophages, respectively. The LPS-induced expression of crucial pro-inflammation cytokines and RANKL-induced osteoclastogenesis as well as the activation of NF-κB pathway in both situations were detected. The inflammation-induced RANKL expression and subsequent inflammatory bone destruction in vivo were determined in collagen-immunized mice. Results: The gold cluster strongly suppresses RANKL-induced osteoclast formation via inhibiting the activation of NF-κB pathway in vitro. Moreover, treatment with the clusters at a dose of 5 mg Au/kg.bw significantly reduces the severity of inflammation-induced bone and cartilage destruction in vivo without any significant toxicity effects. Conclusion: Therefore, the gold clusters may offer a novel potent therapeutic stratagem for inhibiting chronic inflammation associated bone destruction.
- Published
- 2019
11. Characterization of blueberry (Vaccinium corymbosum L.) catechol oxidases III binding mechanism in response to selected substrates and inhibitors
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Yulong Wei, Ning Yu, Yue Zhu, Chengli Jia, Yuhang Xiao, Yue Zhao, Pengju Cai, Wanbin Zhao, Mengmeng Ju, Tongtong Wu, Zhilin Gan, and Aidong Sun
- Subjects
Food Science - Published
- 2022
12. An artificial metalloenzyme for catalytic cancer-specific DNA cleavage and operando imaging
- Author
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Qing Yuan, Lina Zhao, Wenchao Niu, Xiayan Wang, Y. Q. Zhang, Pengju Cai, Huaidong Jiang, Yuhua Tang, Xueyun Gao, Fuping Gao, and Liang Gao
- Subjects
Multidisciplinary ,biology ,Biocompatibility ,Chemistry ,DNA damage ,Substrate (chemistry) ,SciAdv r-articles ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,biology.protein ,Hydroxyl radical ,Health and Medicine ,Bovine serum albumin ,0210 nano-technology ,Hydrogen peroxide ,Research Articles ,Research Article - Abstract
A metal cluster stuck in a protein acts as a synthetic metalloenzyme for cancer-specific catalytic therapy., Metalloenzymes are promising anticancer candidates to overcome chemoresistance by involving unique mechanisms. To date, it is still a great challenge to obtain synthetic metalloenzymes with persistent catalytic performance for cancer-specific DNA cleavage and operando imaging. Here, an artificial metalloenzyme, copper cluster firmly anchored in bovine serum albumin conjugated with tumor-targeting peptide, is exquisitely constructed. It is capable of persistently transforming hydrogen peroxide in tumor microenvironment to hydroxyl radical and oxygen in a catalytic manner. The stable catalysis recycling stems from the electron transfer between copper cluster and substrate with well-matched energy levels. Notably, their high biocompatibility, tumor-specific recognition, and persistent catalytic performance ensure the substantial anticancer efficacy by triggering DNA damage. Meanwhile, by coupling with enzyme-like reactions, the operando therapy effect is expediently traced by chemiluminescence signal with high sensitivity and sustainability. It provides new insights into synthesizing biocompatible metalloenzymes on demand to visually monitor and efficiently combat specific cancers.
- Published
- 2020
13. Is GSH Chelated Pt Molecule Inactive in Anti-Cancer Treatment? A Case Study of Pt
- Author
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Chunyu, Zhang, Liang, Gao, Qing, Yuan, Lina, Zhao, Wenchao, Niu, Pengju, Cai, Jiaojiao, Li, Xu, Han, Zhesheng, He, Fuping, Gao, Yaling, Wang, Huaidong, Jiang, Zhifang, Chai, and Xueyun, Gao
- Subjects
Mice ,Animals ,Humans ,Antineoplastic Agents ,Platinum Compounds ,Triple Negative Breast Neoplasms ,Glutathione ,Survival Analysis ,Platinum - Abstract
Platinum (Pt) drugs are widely used in anti-cancer treatment although many reports advocated that tumor cells could inactivate Pt drugs via glutathione-Pt (GSH-Pt) adducts formation. To date, GSH chelated Pt molecules have not been assessed in cancer treatment because GSH-Pt adducts are not capable of killing cancer cells, which is widely accepted and well followed. In this report, endogenous biothiol is utilized to precisely synthesize a GSH chelated Pt molecule (Pt
- Published
- 2020
14. Volatile components, total phenolic compounds, and antioxidant capacities of worm-infected Gomphidius rutilus
- Author
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Yin Zhang, Bingxin Sun, Zhiyong Zhang, Yunyun Wei, Wei He, Xiaowen Wen, Pengju Cai, Guang Xin, and Libin Sun
- Subjects
Antioxidant ,biology ,medicine.medical_treatment ,fungi ,010401 analytical chemistry ,Gomphidius ,lcsh:TX341-641 ,04 agricultural and veterinary sciences ,biology.organism_classification ,Mass spectrometry ,040401 food science ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Total volatile ,medicine ,Food science ,Rutilus ,Benzene ,lcsh:Nutrition. Foods and food supply ,Food Science ,Worm infection - Abstract
This study evaluated the effects of worm infection on the volatile components, total phenolic compounds, and antioxidant capacities of Gomphidius rutilus. G. rutilus without worms (GW), G. rutilus infected by a small amount of worms (GS; infected area 50%) were investigated. The volatile components of G. rutilus were analyzed by simultaneous distillation–extraction (SDE) and headspace solid-phase microextraction (HS-SPME) using gas chromatography–mass spectrometry (GC–MS). A total of 17 and 19 types of volatile compounds were detected, including ketones, alcohols, benzene, alkenes, aldehydes, esters, acids, and alkanes. Alcohols comprised the most abundant compound in GL, GS, and GW. The relative content of 1-octen-3-ol was the highest in all mushrooms. The concentration of eight-carbon (C8) compounds relative to the total volatile compounds varied widely, ranging from 40% (GW) to 64.34% (GS) and 84.42% (GS) and to 91.59% (GL), respectively, among the three samples. The antioxidant capability and the total phenolic contents of G. rutilus were evaluated in this study. The highest total phenolic content (TPC) of 192.23 mg GAE/g was found in GL, which differed significantly (P < 0.05) from the latter two samples, whereas the lowest value of 156.11 mg GAE/g was found in GW. ABTS radical cation scavenging activity, FRAP ferric reducing antioxidant capacity (FRAP) radical scavenging activity, and oxygen radical absorbance capacity (ORAC) were investigated to screen the antioxidant properties of extracts. The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations (P < 0.01). Among the three types of samples, the phenolic compounds of GL exhibited the highest antioxidant capacity, showing the values of 0.089 mM TE/g for ABTS, 0.949 mM Fe2+ E/g for FRAP, and 1.952 M TE/g for ORAC. However, regarding the total antioxidant capacity, GS exhibited the highest antioxidant capacity, showing the values of 0.002648 mM TE/g for ABTS, 0.004437 mM Fe2+ E/g for FRAP, and 0.256 μM TE/g for ORAC. In conclusion, HS-SPME was more suitable for the extraction of volatile aroma components from G. rutilus. GL had the most abundant aroma components. GL had the highest TPC and antioxidant capacity compared with those of GS and GW, whereas GS showed the opposite results. Interestingly, GS was found to have the highest total antioxidant capacity in vitro. Based on these measured indicators, worm infection had no negative effect on the quality of G. rutilus. Therefore, worm-infected G. rutilus can also be consumed by humans. Keywords: Gomphidius rutilus, Worm infection, Volatile components, Total phenolic, Antioxidant capacity
- Published
- 2018
15. Turning On/Off the Anti-Tumor Effect of the Au Cluster via Atomically Controlling Its Molecular Size
- Author
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Xiangchun Zhang, Shuhong Yi, Yuliang Zhao, Juanjuan Guo, Pengju Cai, Jiao Zhai, Zhifang Chai, Xueyun Gao, Lina Zhao, Fuping Gao, Liang Gao, Qing Yuan, and Yanwei Jia
- Subjects
Surface Properties ,Cell ,Mice, Nude ,General Physics and Astronomy ,Antineoplastic Agents ,Apoptosis ,02 engineering and technology ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Mice ,Structure-Activity Relationship ,Molecular size ,Cell Line, Tumor ,Puma ,Mole ,medicine ,Animals ,Humans ,General Materials Science ,Particle Size ,Cell Proliferation ,Antitumor activity ,Dose-Response Relationship, Drug ,biology ,Chemistry ,General Engineering ,Neoplasms, Experimental ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Molecular biology ,In vitro ,0104 chemical sciences ,medicine.anatomical_structure ,Gold ,Drug Screening Assays, Antitumor ,Peptides ,0210 nano-technology ,Injections, Intraperitoneal - Abstract
We reported two Au clusters with precisely controlled molecular size (Au5Peptide3 and Au22Peptide10) showing different antitumor effects. In vitro, both Au5Peptide3 and Au22Peptide10 were well taken up by human nasopharyngeal cancer cells (CNE1 cells). However, only Au5Peptide3 significantly induced CNE1 cell apoptosis. Further studies showed that CNE1 cells took up Au5Peptide3 (1.98 × 10–15 mol/cell), and 9% of them entered mitochondria (0.186 × 10–15 mol/cell). As a comparison, the uptake of Au22Peptide10 was only half the amount of Au5Peptide3 (1.11 × 10–15 mol/cell), and only 1% of them entered mitochondria (0.016 × 10–15 mol/cell). That gave 11.6-fold more Au5Peptide3 in mitochondria of CNE1 cells than Au22Peptide10. Further cell studies revealed that the antitumor effect may be due to the enrichment of Au5Peptide3 in mitochondria. Au5Peptide3 slightly decreased the Mcl-1 (antiapoptotic protein of mitochondria) and significantly increased the Puma (pro-apoptotic protein of mitochondria) expression le...
- Published
- 2018
16. Surface-Functionalized Modified Copper Sulfide Nanoparticles Enhance Checkpoint Blockade Tumor Immunotherapy by Photothermal Therapy and Antigen Capturing
- Author
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Xueyun Gao, Zhesheng He, Liang Gao, Yao Zhao, Yuliang Zhao, Fuping Gao, Pengju Cai, Ruoping Wang, and Wenzhi Yang
- Subjects
Hyperthermia ,Materials science ,Cell Survival ,medicine.medical_treatment ,Breast Neoplasms ,02 engineering and technology ,CD8-Positive T-Lymphocytes ,010402 general chemistry ,Antibodies, Monoclonal, Humanized ,01 natural sciences ,B7-H1 Antigen ,Proinflammatory cytokine ,Metastasis ,Polyethylene Glycols ,Maleimides ,Mice ,Immune system ,Antigen ,Cell Line, Tumor ,medicine ,Animals ,Humans ,General Materials Science ,Mice, Inbred BALB C ,Lasers ,technology, industry, and agriculture ,Antibodies, Monoclonal ,Immunotherapy ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,medicine.disease ,Immune checkpoint ,0104 chemical sciences ,Cancer research ,Cytokines ,Nanoparticles ,Female ,0210 nano-technology ,Copper - Abstract
Nanomaterial-based tumor photothermal therapy (PTT) has attracted increasing attention and been a promising method for cancer treatment because of its low level of adverse effects and noninvasiveness. However, thermotherapy alone still cannot control tumor metastasis and recurrence. Here, we developed surface-functionalized modified copper sulfide nanoparticles (CuS NPs). CuS NPs can not only be used as photothermal mediators for tumor hyperthermia but can adsorb tumor antigens released during hyperthermia as an antigen-capturing agent to induce antitumor immune response. We selected maleimide polyethylene glycol-modified CuS NPs (CuS NPs-PEG-Mal) with stronger antigen adsorption capacity, in combination with an immune checkpoint blocker (anti-PD-L1) to evaluate the effect of hyperthermia, improving immunotherapy in a 4T1 breast cancer tumor model. The results showed that hyperthermia based on CuS NPs-PEG-Mal distinctly increased the levels of inflammatory cytokines in the serum, leading to a tumor immunogenic microenvironment. In cooperation with anti-PD-L1, PTT mediated by CuS NPs-PEG-Mal enhanced the number of tumor-infiltrating CD8+ T cells and inhibited the growth in primary and distant tumor sites of the 4T1 tumor model. The therapeutic strategies provide a simple and effective treatment option for metastatic and recurrent tumors.
- Published
- 2019
17. Peptide-Templated Gold Clusters as Enzyme-Like Catalyst Boost Intracellular Oxidative Pressure and Induce Tumor-Specific Cell Apoptosis
- Author
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Chunyu Zhang, Liang Gao, Xiaojun Ren, Zhichao Zhang, Yuhua Tang, Wenchao Niu, Jiaojiao Li, Xueyun Gao, Qing Yuan, Xiangchun Zhang, Zhesheng He, Y. Q. Zhang, and Pengju Cai
- Subjects
biocatalysis ,General Chemical Engineering ,Peptide ,02 engineering and technology ,Oxidative phosphorylation ,oxidative pressure-type tumor ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,gold clusters ,Article ,lcsh:Chemistry ,chemistry.chemical_compound ,medicine ,General Materials Science ,Hydrogen peroxide ,chemistry.chemical_classification ,reactive oxygen species ,Reactive oxygen species ,Chemistry ,Superoxide ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,lcsh:QD1-999 ,Cancer cell ,Biophysics ,0210 nano-technology ,Intracellular ,Oxidative stress - Abstract
Anticancer metallodrugs that aim to physiological characters unique to tumor microenvironment are expected to combat drug tolerance and side-effects. Recently, owing to the fact that reactive oxygen species&rsquo, is closely related to the development of tumors, people are committed to developing metallodrugs with the capacity of improving the level of reactive oxygen species level toinduce oxidative stress in cancer cells. Herein, we demonstrated that peptide templated gold clusters with atomic precision preferably catalyze the transformation of hydrogen peroxide into superoxide anion in oxidative pressure-type tumor cells. Firstly, we successfully constructed gold clusters by rationally designing peptide sequences which targets integrin &alpha, &nu, &beta, 3 overexpressed on glioblastoma cells. The superoxide anion, radical derived from hydrogen peroxide and catalyzed by gold clusters, was confirmed in vitro under pseudo-physiological conditions. Then, kinetic parameters were evaluated to verify the catalytic properties of gold clusters. Furthermore, these peptide decorated clusters can serve as special enzyme-like catalyst to convert endogenous hydrogen peroxide into superoxide anion, elevated intracellular reactive oxygen species levels, lower mitochondrial membrane potential, damage biomacromolecules, and trigger tumor cell apoptosis consequently.
- Published
- 2018
18. Is GSH Chelated Pt Molecule Inactive in Anti‐Cancer Treatment? A Case Study of Pt6GS4
- Author
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Xu Han, Wenchao Niu, Fuping Gao, Jiaojiao Li, Liang Gao, Yaling Wang, Qing Yuan, Chunyu Zhang, Xueyun Gao, Huaidong Jiang, Zhesheng He, Zhifang Chai, Lina Zhao, and Pengju Cai
- Subjects
02 engineering and technology ,General Chemistry ,Glutathione ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Carboplatin ,In vitro ,0104 chemical sciences ,Biomaterials ,chemistry.chemical_compound ,chemistry ,In vivo ,Apoptosis ,Cancer cell ,Cancer research ,General Materials Science ,Chelation ,0210 nano-technology ,Triple-negative breast cancer ,Biotechnology - Abstract
Platinum (Pt) drugs are widely used in anti-cancer treatment although many reports advocated that tumor cells could inactivate Pt drugs via glutathione-Pt (GSH-Pt) adducts formation. To date, GSH chelated Pt molecules have not been assessed in cancer treatment because GSH-Pt adducts are not capable of killing cancer cells, which is widely accepted and well followed. In this report, endogenous biothiol is utilized to precisely synthesize a GSH chelated Pt molecule (Pt(6)GS(4)). This Pt(6)GS(4) molecule can be well taken up by aggressive triple negative breast cancer (TNBC) cells. Subsequently, its metabolites could enter nuclei to interact with DNA, finally the DNA-Pt complex triggers TNBC cell apoptosis via the p53 pathway. Impressively, high efficacy for anti-cancer treatment is achieved by Pt(6)GS(4) both in vitro and in vivo when compared with traditional first-line carboplatin in the same dosage. Compared with carboplatin, Pt(6)GS(4) keeps tumor bearing mice alive for a longer time and is non-toxic for the liver and kidneys. This work opens a route to explore polynuclear Pt compound with accurate architecture for enhancing therapeutic effects and reducing systemic toxicity.
- Published
- 2020
19. Photodynamic Therapy: Au Nanoclusters and Photosensitizer Dual Loaded Spatiotemporal Controllable Liposomal Nanocomposites Enhance Tumor Photodynamic Therapy Effect by Inhibiting Thioredoxin Reductase (Adv. Healthcare Mater. 7/2017)
- Author
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Yuliang Zhao, Pengju Cai, Qing Yuan, Liang Gao, Xueyun Gao, Ru Liu, Weiping Zheng, Fuping Gao, and Yaling Wang
- Subjects
chemistry.chemical_classification ,Reactive oxygen species ,Liposome ,Nanocomposite ,Chemistry ,medicine.medical_treatment ,Thioredoxin reductase ,Biomedical Engineering ,Pharmaceutical Science ,Photodynamic therapy ,Photochemistry ,Nanoclusters ,Biomaterials ,medicine ,Biophysics ,Photosensitizer - Published
- 2017
20. Au Clusters Treat Rheumatoid Arthritis with Uniquely Reversing Cartilage/Bone Destruction
- Author
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Pengju Cai, Meiqing Liu, Fuping Gao, Liang Gao, Yawen Yao, Zhifang Chai, Qing Yuan, Xueyun Gao, and Lina Zhao
- Subjects
rheumatoid arthritis ,General Chemical Engineering ,General Physics and Astronomy ,Medicine (miscellaneous) ,Inflammation ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Proinflammatory cytokine ,Au clusters ,Downregulation and upregulation ,Osteoclast ,In vivo ,medicine ,General Materials Science ,lcsh:Science ,Full Paper ,biology ,Chemistry ,Cartilage ,General Engineering ,Full Papers ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,bone destruction ,medicine.anatomical_structure ,inflammation ,RANKL ,Rheumatoid arthritis ,biology.protein ,Cancer research ,lcsh:Q ,medicine.symptom ,0210 nano-technology - Abstract
Super‐small nanoclusters may intrinsically trigger specific molecular pathway for disease treatment in vitro/vivo. To prove the hypothesis the super‐small nanoclusters, e.g., Au clusters, are directly used to treat rheumatoid arthritis (RA) in vitro/vivo. RA is a chronic autoimmune disease that is characterized by the inflammation of joints and the unreversible destruction of the cartilage/bone. Au clusters significantly suppress lipopolysaccharide (LPS)‐induced proinflammatory mediator production in the murine macrophage cell line by inhibiting the signaling pathways that regulate the major proinflammatory mediator genes. In preclinical rat RA studies, Au clusters strongly prevent type II collagen‐induced rat RA without systemic side effects. Compared with the clinical first‐line anchored anti‐RA drug, methotrexate, Au clusters equally inhibit inflammation in vivo. Type II collagen‐induced rat RA is characterized with the destruction of cartilage/bone; treatment with Au clusters reverses the destruction of cartilage/bone to its normal state. This is because Au clusters directly inhibit receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclast differentiation and function through the downregulation of osteoclast‐specific genetic marker expression. However the methotrexate almost has no positive effect for this key issue in rat RA therapy. These data prove that the super‐small nanoclusters, e.g., Au clusters, could be a novel candidate nanodrug for RA treatment.
- Published
- 2019
21. Inherently PET/CT Dual Modality Imaging Lipid Nanocapsules for Early Detection of Orthotopic Lung Tumors.
- Author
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Pengju Cai, Dongdong Su, Wenjiang Yang, Zhesheng He, Chunyu Zhang, Hui Liu, Zhibo Liu, Xiangchun Zhang, Liang Gao, Yu Liu, Huaidong Jiang, Fuping Gao, and Xueyun Gao
- Published
- 2020
- Full Text
- View/download PDF
22. Ultrasmall [(64)Cu]Cu nanoclusters for targeting orthotopic lung tumors using accurate positron emission tomography imaging
- Author
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Zhifang Chai, Guodong Huang, Jingquan Xue, Fuping Gao, Liang Gao, Yawei Zhao, Wenjiang Yang, Xiao He, Xueyun Gao, Lina Zhao, Fasheng Wu, Yuliang Zhao, Pengju Cai, Yaling Wang, and Ru Liu
- Subjects
Models, Molecular ,Fluorescence-lifetime imaging microscopy ,Materials science ,Lung Neoplasms ,Protein Conformation ,General Physics and Astronomy ,Metal Nanoparticles ,Nanotechnology ,Nanoclusters ,Mice ,Nuclear magnetic resonance ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,General Materials Science ,Chelation ,Tissue Distribution ,Bovine serum albumin ,Particle Size ,Chelating Agents ,Mice, Inbred BALB C ,biology ,medicine.diagnostic_test ,General Engineering ,Serum Albumin, Bovine ,Nanomedicine ,Copper Radioisotopes ,Microscopy, Fluorescence ,Positron emission tomography ,Positron-Emission Tomography ,biology.protein ,Cattle ,Female ,Molecular imaging - Abstract
Positron emission tomography (PET) imaging has received special attention owing to its higher sensitivity, temporal resolution, and unlimited tissue penetration. The development of tracers that target specific molecules is therefore essential for the development and utility of clinically relevant PET procedures. However, (64)Cu as a PET imaging agent generally has been introduced into biomaterials through macrocyclic chelators, which may lead to the misinterpretation of PET imaging results due to the detachment and transchelation of (64)Cu. In this study, we have developed ultrasmall chelator-free radioactive [(64)Cu]Cu nanoclusters using bovine serum albumin (BSA) as a scaffold for PET imaging in an orthotopic lung cancer model. We preconjugated the tumor target peptide luteinizing hormone releasing hormone (LHRH) to BSA molecules to prepare [(64)Cu]CuNC@BSA-LHRH. The prepared [(64)Cu]Cu nanoclusters showed high radiolabeling stability, ultrasmall size, and rapid deposition and diffusion into tumor, as well as predominantly renal clearance. [(64)Cu]CuNC@BSA-LHRH showed 4 times higher tumor uptake compared with that of [(64)Cu]CuNC@BSA by analyzing the (64)Cu radioactivity of tissues via gamma counting. The PET imaging using [(64)Cu]Cu nanoclusters as tracers showed more sensitive, accurate, and deep penetration imaging of orthotopic lung cancer in vivo compared with near-infrared fluorescence imaging. The nanoclusters provide biomedical research tools for PET molecular imaging.
- Published
- 2015
23. Au Nanoclusters and Photosensitizer Dual Loaded Spatiotemporal Controllable Liposomal Nanocomposites Enhance Tumor Photodynamic Therapy Effect by Inhibiting Thioredoxin Reductase
- Author
-
Yuliang Zhao, Liang Gao, Ru Liu, Pengju Cai, Weiping Zheng, Xueyun Gao, Qing Yuan, Yaling Wang, and Fuping Gao
- Subjects
Thioredoxin-Disulfide Reductase ,Thioredoxin reductase ,medicine.medical_treatment ,Biomedical Engineering ,Metal Nanoparticles ,Mice, Nude ,Pharmaceutical Science ,Breast Neoplasms ,Photodynamic therapy ,02 engineering and technology ,Photochemistry ,Nanocomposites ,Nanoclusters ,Biomaterials ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,medicine ,Animals ,Humans ,Photosensitizer ,Lipid bilayer ,Mice, Inbred BALB C ,Liposome ,Photosensitizing Agents ,Chemistry ,021001 nanoscience & nanotechnology ,Xenograft Model Antitumor Assays ,Neoplasm Proteins ,Photochemotherapy ,030220 oncology & carcinogenesis ,Liposomes ,MCF-7 Cells ,Biophysics ,Female ,Gold ,Thioredoxin ,0210 nano-technology - Abstract
Photodynamic therapy (PDT) is a minimally invasive therapeutic procedure of tumors with high selectivity and low side effect. However, it is usually not efficient in long-lasting tumor control. One of the main reasons is tumor cells develop some protective mechanisms that help them to deal with oxidative stress in the environment. The thioredoxin system in cancer is an important antioxidant defense system. Au nanoclusters could effectively inhibit thioredoxin reductase (TrxR) in tumor cell cytoplasm. Herein, Au nanoclusters and photosensitizer Chlorine 6 (Ce6) are co-loaded in spatiotemporal controllable liposomal nanocomposites. pH responsive molecule inserted in lipid bilayer greatly contributes to the instability of the lipid membrane in lysosomal at low pH environment. Then the payloads can rapidly release into cytoplasm. Au nanoclusters effectively inhibit TrxR in cytoplasm and enhance the photodynamic-induced intracellular reactive oxygen-free radical concentration, improving the effect of PDT. Breast cancer is chosen as a tumor model and the Au nanoclusters and photosensitizer co-loaded liposomal nanocomposites are studied to improve the effect of PDT both in vitro and in vivo, and its corresponding mechanism is investigated. This study develops a new application of gold nanoclusters and provides a new train of thoughts for enhancing the effect of PDT.
- Published
- 2017
24. Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles
- Author
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Guodong Huang, Liang Gao, Jian Liang, Huarui Zhu, Yaling Wang, Qing Yuan, Yueteng Wei, Xueyun Gao, Fuping Gao, Pengju Cai, and Ru Liu
- Subjects
Antioxidant ,Materials science ,medicine.medical_treatment ,Biophysics ,chemistry.chemical_element ,Mice, Nude ,Bioengineering ,Antineoplastic Agents ,Apoptosis ,Pharmacology ,Irinotecan ,Biomaterials ,Selenium ,Cell Line, Tumor ,Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Animals ,Humans ,Cytotoxicity ,Chemotherapy ,Mice, Inbred BALB C ,technology, industry, and agriculture ,Bioavailability ,Rats ,chemistry ,Mechanics of Materials ,Toxicity ,Ceramics and Composites ,Nanoparticles ,Camptothecin ,Female ,medicine.drug - Abstract
Although chemotherapeutic drugs are widely applied for clinic tumor treatment, severe toxicity restricts their therapeutic efficacy. In this study, we reported a new form of selenium, selenium nanoparticles (Nano Se) which have significant lower toxicity and acceptable bioavailability. We investigated Nano Se as chemotherapy preventive agent to protect against toxicities of anticancer drug irinotecan and synergistically enhance the anti-tumor treatment effect in vitro and in vivo. The underlying mechanisms were also investigated. The combination of Nano Se and irinotecan showed increased cytotoxic effect with HCT-8 tumor cells likely by p53 mediated apoptosis. Nano Se inhibited growth of HCT-8 tumor cells partially through caspases mediated apoptosis. In vivo experiment showed Nano Se at a dose of 4 mg/kg/day significantly alleviated adverse effects induced by irinotecan (60 mg/kg) treatment. Nano Se alone treatment did not induce any toxic manifestations. The combination of Nano Se and irinotecan dramatically inhibited tumor growth and significantly induced apoptosis of tumor cells in HCT-8 cells xenografted tumor. Tumor inhibition rate was about 17.2%, 48.6% and 62.1% for Nano Se, irinotecan and the combination of Nano Se and irinotecan, respectively. The beneficial effects of Nano Se for tumor therapy were mainly ascribed to selectively regulating Nrf2-ARE (antioxidant responsive elements) pathway in tumor tissues and normal tissues. Our results suggest Nano Se is a promising selenium species with potential application in cancer treatment.
- Published
- 2014
25. An artificial metalloenzyme for catalytic cancer-specific DNA cleavage and operando imaging.
- Author
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Liang Gao, Ya Zhang, Lina Zhao, Wenchao Niu, Yuhua Tang, Fuping Gao, Pengju Cai, Qing Yuan, Xiayan Wang, Huaidong Jiang, and Xueyun Gao
- Subjects
- *
DEOXYRIBOZYMES , *PARTICLE physics , *PHYSICAL sciences , *HABER-Weiss reaction , *GOLD clusters , *MEDICAL sciences , *SERUM albumin - Abstract
The article presents health and medicine research on artificial metalloenzyme for catalytic cancer-specific DNA cleavage and operando imaging. Topics including metalloenzymes are promising anticancer candidates to overcome chemoresistance by involving unique mechanisms; and challenge to obtain synthetic metalloenzymes with persistent catalytic performance for cancer-specific DNA cleavage and operando imaging.
- Published
- 2020
- Full Text
- View/download PDF
26. Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles.
- Author
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Fuping Gao, Qing Yuan, Liang Gao, Pengju Cai, Huarui Zhu, Ru Liu, Yaling Wang, Yueteng Wei, Guodong Huang, Jian Liang, and Xueyun Gao
- Subjects
- *
IRINOTECAN , *NANOMEDICINE , *DRUG toxicity , *PHYSIOLOGICAL effects of selenium , *DRUG efficacy , *TUMOR treatment , *CANCER chemotherapy , *THERAPEUTICS - Abstract
Although chemotherapeutic drugs are widely applied for clinic tumor treatment, severe toxicity restricts their therapeutic efficacy. In this study, we reported a new form of selenium, selenium nanoparticles (Nano Se) which have significant lower toxicity and acceptable bioavailability. We investigated Nano Se as chemotherapy preventive agent to protect against toxicities of anticancer drug irinotecan and synergistically enhance the anti-tumor treatment effect in vitro and in vivo . The underlying mechanisms were also investigated. The combination of Nano Se and irinotecan showed increased cytotoxic effect with HCT-8 tumor cells likely by p53 mediated apoptosis. Nano Se inhibited growth of HCT-8 tumor cells partially through caspases mediated apoptosis. In vivo experiment showed Nano Se at a dose of 4 mg/kg/day significantly alleviated adverse effects induced by irinotecan (60 mg/kg) treatment. Nano Se alone treatment did not induce any toxic manifestations. The combination of Nano Se and irinotecan dramatically inhibited tumor growth and significantly induced apoptosis of tumor cells in HCT-8 cells xenografted tumor. Tumor inhibition rate was about 17.2%, 48.6% and 62.1% for Nano Se, irinotecan and the combination of Nano Se and irinotecan, respectively. The beneficial effects of Nano Se for tumor therapy were mainly ascribed to selectively regulating Nrf2-ARE (antioxidant responsive elements) pathway in tumor tissues and normal tissues. Our results suggest Nano Se is a promising selenium species with potential application in cancer treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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