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Objective. To study indicators of treatment of schizophrenia in different stages of psychiatric care, to reveal prognostic factors of the dynamics of psychopathological symptoms in treatment of schizophrenia and to estimate the risk of schizophrenia relapse. Material and methods. Three hundreds of patients, aged 18-65 years, diagnosed with schizophrenia (ICD-10) were included in data analysis. Patients were stratified into 4 groups by the type of psychiatric care: inpatients (n=68), outpatients of day hospital (n=71), outpatients of psychoneurological dispensary rehabilitation department (n=75) and outpatients supervised by psychoneurological dispensary local doctor. Treatment characteristics (use of 1st and 2nd generation antipsychotics) of these groups were compared. Results and conclusion. The positive effect of modern antipsychotics on schizophrenia course, frequency of relapses and hospitalizations was identified. The results can help in improvement of psychiatric care due to wide implementation of 2nd generation antipsychotics.

The potato tubers contain proteins that inhibit serine proteinases and belong to subfamily of potato Kunitz-type proteinase inhibitors (PKPI). New highly purified protein had been isolated from mature potato tubers (Solanum tuberosum L., cv. Zukov's Jubilee). The protein is а single polypeptide chain of molecular, weighing 23 kDa. The 20 N-terminal amino acid residues of the protein were determined by automatic Edman procedure. On the basis of N-terminal sequence structure and the molecular mass it is indicated that the inhibitor is a potato Kunitz-type serine proteinase inhibitor that belongs to group B. It was denoted as PKCI (potato Kunitz-type chymotrypsin inhibitor). The PKCI was able to inhibit chymotrypsin and trypsin with the same degree of effectiveness. It formed equimolar complexes with both enzymes. The protein PKCI is a double-headed proteinase inhibitor and is able to bind simultaneously two molecules of different enzymes. The probable cDNA of the inhibitor, denoted as PKPIJ-B consists of a 579-bp open reading frame, encodes protein out of 193-amino acids with a signal peptide (10 residues), indicating that this protein is synthesized as a preprotein. Deduced amino acid sequence of the cDNA is aligned with the respective sequences of already known PKPI, belonging to group B., (Copyright © 2011 Elsevier Masson SAS. All rights reserved.)

Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor) of potato tubers.

Potato Kunitz-type chymotrypsin inhibitor (PKCI-23) was isolated from potato tubers (Solanum tuberosum L., Zhukov's Jubilee breed) and purified to a homogenous state. The protein was purified by gel-filtration chromatography and ion-exchange chromatography using Sephadex G-75 and CM-Sepharose CL-6B, respectively. PKCI-23 protein has been shown to inhibit both chymotrypsin and trypsin with equal efficacy, forming equimolar complexes with these enzymes. However, much weaker inhibitory effect of PKCI-23 has been observed for Carlsberg subtilisin. The N-terminal 20 amino acid sequence of PKCI-23 has been sequenced. PKCI-23 has been shown to suppress, with different efficacy, the growth and development of pathogenic microorganisms Fusarium culmorum (Wm. G. Sm.) Sacc. and Phytophtora infestans (Mont.) de Bary that infect potato.