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[Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers].

Authors :
Parfenov IA
Revina TA
PashkovskiÄ­ PP
Radiukina NL
Valueva TA
Source :
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2011 Jul-Aug; Vol. 47 (4), pp. 402-7.
Publication Year :
2011

Abstract

Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor) of potato tubers.

Details

Language :
Russian
ISSN :
0555-1099
Volume :
47
Issue :
4
Database :
MEDLINE
Journal :
Prikladnaia biokhimiia i mikrobiologiia
Publication Type :
Academic Journal
Accession number :
21950113