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[Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers].
- Source :
-
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2011 Jul-Aug; Vol. 47 (4), pp. 402-7. - Publication Year :
- 2011
-
Abstract
- Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor) of potato tubers.
- Subjects :
- Amino Acid Sequence
Amino Acid Substitution
Chymotrypsin metabolism
DNA Primers
Molecular Sequence Data
Phylogeny
Plant Proteins genetics
Plant Proteins metabolism
Plant Tubers chemistry
Plant Tubers enzymology
Polymorphism, Genetic
Protein Isoforms genetics
Protein Isoforms metabolism
Sequence Homology, Amino Acid
Solanum tuberosum chemistry
Solanum tuberosum enzymology
Trypsin metabolism
Trypsin Inhibitors genetics
Trypsin Inhibitors metabolism
DNA chemistry
Plant Proteins chemistry
Plant Tubers genetics
Protein Isoforms chemistry
Solanum tuberosum genetics
Trypsin Inhibitors chemistry
Subjects
Details
- Language :
- Russian
- ISSN :
- 0555-1099
- Volume :
- 47
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Prikladnaia biokhimiia i mikrobiologiia
- Publication Type :
- Academic Journal
- Accession number :
- 21950113