10 results on '"Paravani, E. V."'
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2. DNA damage, alterations in the expression of antioxidant enzyme genes and in the histoarchitecture of gill cells of zebrafish exposed to 17-α-ethinylestradiol.
- Author
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Paravani, E. V., Bianchi, M., Querubín Pereyra, P. L., Acosta, M. G., Odetti, L., Simoniello, M. F., and Poletta, G.
- Subjects
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GENE expression , *SUPEROXIDE dismutase , *POLLUTANTS , *NON-target organisms , *DNA damage , *GILLS , *BRACHYDANIO - Abstract
Endocrine disruptors, such as estrogen, are chemical substances with the potential to alter the hormonal balance of organisms. Their origin can be natural or artificial, and they can act at very low doses. The estrogen 17α-ethinylestradiol (EE2) is used worldwide as an oral contraceptive and is a potential contaminant in aquatic ecosystems. It is well documented that these environmental pollutants can act directly or indirectly on the reproductive system, impairing development and fertility. However, little is known about the alteration of the cell oxidative status induced by EE2. The main objective of this study was to evaluate the effect on the gill cells of adult zebrafish exposed in vivo to EE2, analyzing cell histology, DNA damage and the expression levels of genes encoding the main enzymes involved in oxidative stress pathways. The histological study showed that EE2 produces moderate to high damage to the gill tissue, an increase in gill cell DNA damage and the mRNA levels of the genes corresponding to the manganese superoxide dismutase (Mn-sod) and catalase (cat) after exposure to 5 ng/L EE2. The results indicate that EE2 causes tissue alterations, DNA damage and oxidative stress. EE2 produced important alterations in the gills, a fundamental organ for the survival of fish. There is a clear need for further research on the ecological consequences of EDCs on non-target organisms. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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3. DNA damage, alterations in the expression of antioxidant enzyme genes and in the histoarchitecture of gill cells of zebrafish exposed to 17-α-ethinylestradiol
- Author
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Paravani, E. V., primary, Bianchi, M., additional, Querubín Pereyra, P. L., additional, Acosta, M. G., additional, Odetti, L., additional, Simoniello, M. F., additional, and Poletta, G., additional
- Published
- 2023
- Full Text
- View/download PDF
4. PID 6088 Estudio de la influencia de las hormonas tiroideas en el control de los sistemas de adhesión cadherinas-cateninas durante el desarrollo de vertebrados
- Author
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Galetto, C. D., Diaz Zamboni, J. E., Adur, Javier Fernando, Vicente, N. B., Larrea, D., Bessone, M. V., Hasenahuer, Marcia Anahí, Paravani, E. V., Bianchi, Mariana, Casco, Victor Hugo, and Izaguirre, María Fernanda
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Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,Otras Ciencias Biológicas ,Hormonas tiroideas ,Cateninas ,Desarrollo ,purl.org/becyt/ford/1.6 [https] ,CIENCIAS NATURALES Y EXACTAS ,Cadherinas - Abstract
El desarrollo de organismos pluricelulares depende en gran medida del establecimiento y mantenimiento de contactos adhesivos fuertes, pero a la vez dinámicos para posibilitar el remodelamiento tisular frente a señales específicas, tanto durante el desarrollo como en el estado adulto. Interesados en evaluar mecanismos de control hormonal de estos contactos, estudiamos si las hormonas tiroideas eran capaces de controlar el desarrollo animal a través de la regulación de la expresión de moléculas de adhesión celular (CAMs), las cuales influencian la adhesión celular y la morfología celular y tisular. Para ello, en una primera etapa, se implementaron bioensayos de bloqueo e inducción de la metamorfosis de Rhinella arenarum y se analizó por inmunohistoquímica cuantitativa el patrón de expresión de las CAMs cadherina E, b- y a-catenina en el intestino anterior o estómago larval de esta especie. En una segunda etapa se utilizaron técnicas de inmunofluorescencia y microscopia de desconvolución digital tridimensional cuantitativa para analizar la influencia de los niveles de fosforilación de las proteínas de los complejos de unión cadherina-catenina in vivo. En una tercera etapa, se analizaron los niveles de expresión de los ARNm de cadherina E y β-catenina y de sus proteínas para correlacionar los estudios morfométricos realizados con estudios moleculares, empleando retrotranscripción y amplificación de ADNcopia (ADNc) por reacción en cadena de la polimerasa (RT-PCR) y western blotting, para, respectivamente. Para ello, se realizaron análisis bioinformáticos de las secuencias y estructuras de las moléculas bajo estudio. Los resultados obtenidos permiten postular por primera vez en forma cuantitativa, un control positivo espacial y temporal de cadherina E, b- y a-catenina por la hormona T3 durante el desarrollo metamórfico del estómago larval de Rhinella arenarum. La alteración de los niveles de fosforilación de las proteínas de los complejos de unión cadherina E-β-catenina, produce una drástica pérdida de estas moléculas en los contactos célula-célula y el incremento citoplasmático y nuclear de β-catenina en las células epidérmicas, sugiriendo la activación de la ruta de señalización nuclear mediada por β-catenina. Sorprendentemente, no se detectan cambios en la forma celular o en la arquitectura de la piel, sugiriendo que la cadherina E epidérmica estaría involucrada en la señalización celular más que en el mantenimiento de los contactos intercelulares durante el mantenimiento de la arquitectura epitelial in vivo. Finalmente, se aislaron, secuenciaron y caracterizaron filogenéticamente secuencias de nucleótidos de cadherina E y de b-catenina de Rhinella arenarum, que resultaron estar altamente conservadas rentre 8 especies de vertebrados. Fil: Galetto, C. D.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Diaz Zamboni, J. E.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Vicente, N. B.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Larrea, D.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Bessone, M. V.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Hasenahuer, Marcia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Paravani, E. V.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Bianchi, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina Fil: Izaguirre, María Fernanda. Universidad Nacional de Entre Ríos. Facultad de Ingeniería. Departamento de Biología. Laboratorio de Microscopia; Argentina
- Published
- 2014
5. Phytotoxicity of one commercial formulated glyphosate on lemna gibba L,Fitotoxicidad de un formulado comercial de glifosato sobre Lemna gibba L
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Sione, S. M. J., Ramírez, A. C., Maria Carolina Sasal, Paravani, E. V., Wilson, M. G., Gabioud, E. A., Polla, W., Repetti, M. R., and Oszust, J. D.
6. Monitoring of glyphosate in surface water in the province of Entre Ríos. Participatory action research as a collaborative methodology,Monitoreo de glifosato en agua superficial en Entre Ríos. La investigación acción participativa como metodología de abordaje
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Maria Carolina Sasal, Wilson, M. G., Sione, S. M., Beghetto, S. M., Gabioud, E. A., Oszust, J. D., Paravani, E. V., Demonte, L., Repetti, M. R., Bedendo, D. J., Medero, S. L., Goette, J. J., Pautasso, N., and Schulz, G. A.
7. Micronucleus test in reptiles: Current and future perspectives.
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Odetti LM, Paravani EV, Simoniello MF, and Poletta GL
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- Animals, Mutagens toxicity, Ecotoxicology methods, DNA Damage drug effects, Sentinel Species genetics, Reptiles genetics, Micronucleus Tests methods
- Abstract
Micronucleus (MN) cell counting emerged in 1973-1975 as a valid alternative for characterizing chromosomal damage caused by different agents. It was first described in mammals, but its application was rapidly extended to other vertebrates, mainly fish. However, it was not until 28 years later that this test was implemented in studies on reptiles. Nowadays, reptiles are found to be excellent non-target species from environmental contamination exposure and MN test has become a fundamental tool for analyzing genotoxic effects induced by various xenobiotics. In this article we provide an updated review of the application of the MN test in reptile species, from an ecotoxicological perspective. Therefore, we present (I) a bibliometric analysis of the available research on genotoxic-induced MN formation in reptile species; (II) the use of reptiles as sentinel organisms in ecotoxicological studies; and (III) the strength and weakness of the application of the MN test in this group. With this review, we aim to provide a comprehensive view on the use of the MN test in ecotoxicology and to encourage further studies involving reptile species., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
8. Pesticide-induced alterations in zebrafish (Danio rerio) behavior, histology, DNA damage and mRNA expression: An integrated approach.
- Author
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Bianchi M, Paravani EV, Acosta MG, Odetti LM, Simoniello MF, and Poletta GL
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- Animals, Zebrafish genetics, Zebrafish metabolism, Glyphosate, DNA Damage, 2,4-Dichlorophenoxyacetic Acid, RNA, Messenger metabolism, Gills metabolism, Pesticides toxicity, Water Pollutants, Chemical metabolism, Neonicotinoids, Nitro Compounds
- Abstract
To assess the impact of glyphosate and 2,4-D herbicides, as well as the insecticide imidacloprid, both individually and in combination, the gills of adult zebrafish were used due to their intimate interaction with chemicals diluted in water. Bioassays were performed exposing the animals to the different pesticides and their mixture for 96 h. The behavior of the fish was analyzed, a histological examination of the gills was carried out, and the genotoxic effects were also analyzed by means of the comet assay (CA) and the change in the expression profiles of genes involved in the pathways of the oxidative stress and cellular apoptosis. The length traveled and the average speed of the control fish, compared to those exposed to the pesticides and mainly those exposed to the mixture, were significantly greater. All the groups exposed individually exhibited a decrease in thigmotaxis time, indicating a reduction in the behavior of protecting themselves from predators. Histological analysis revealed significant differences in the structures of the gill tissues. The quantification of the histological lesions showed mild lesions in the fish exposed to imidacloprid, moderate to severe lesions for glyphosate, and severe lesions in the case of 2,4-D and the mixture of pesticides. The CA revealed the sensitivity of gill cells to DNA damage following exposure to glyphosate, 2,4-D, imidacloprid and the mixture. Finally, both genes involved in the oxidative stress pathway and those related to the cell apoptosis pathway were overexpressed, while the ogg1 gene, involved in DNA repair, was downregulated., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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9. Cypermethrin induction of DNA damage and oxidative stress in zebrafish gill cells.
- Author
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Paravani EV, Simoniello MF, Poletta GL, and Casco VH
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- Animals, Catalase genetics, Catalase metabolism, Gene Expression drug effects, Gills metabolism, Superoxide Dismutase genetics, Superoxide Dismutase metabolism, Zebrafish, DNA Damage drug effects, Gills drug effects, Insecticides toxicity, Oxidative Stress drug effects, Pyrethrins toxicity, Water Pollutants, Chemical toxicity
- Abstract
Cypermethrin (CYP) is a synthetic pyrethroid insecticide, used to control pests in domestic, industrial and agricultural environments. According to recent reports, it is one of the most common contaminants in freshwater aquatic systems. The aim of this study was to evaluate its potential genotoxic effect and the activation of the superoxide dismutase (SOD) and catalase (CAT) systems of adult zebrafish gill cells after in vivo exposure. The comet assay (CA) demonstrated that gill cells are sensitive to DNA damage after in vitro exposure to hydrogen peroxide (H
2 O2 ), showing a dose-dependent response. We also found an increase in DNA damage of gill cells following a dose- and time-dependent treatment with CYP. Moreover, it was verified that SOD and CAT activities significantly increased after exposure to 0.6 µg/L CYP, both during six and nine days. The same treatment caused a significant up-regulation of the mRNA levels of Mn-sod and cat genes. These data indicate that CYP causes gill cell's DNA damage and oxidative stress, modifying the activities of the enzymes responsible for maintaining ROS balance, as well as in their corresponding gene expression levels., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
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10. Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish.
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Paravani EV, Simoniello MF, Poletta GL, Zolessi FR, and Casco VH
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- Animals, Caspase 3 metabolism, Cell Survival, Dose-Response Relationship, Drug, Histones metabolism, Hydrogen Peroxide adverse effects, Mutagenicity Tests, Photoreceptor Cells, Vertebrate drug effects, Photoreceptor Cells, Vertebrate metabolism, Zebrafish, DNA Damage, Oxidative Stress, Photoreceptor Cells, Vertebrate cytology, Pyrethrins toxicity, Water Pollutants, Chemical toxicity
- Abstract
Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, a marker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker, was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxide-induced DNA damage, showing a dose-dependent response. We observed a positive comet assay response to CM that was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and cat mRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induce apoptosis in retinal cells., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
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