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Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish.
- Source :
-
Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2018 Feb; Vol. 826, pp. 25-32. Date of Electronic Publication: 2017 Dec 27. - Publication Year :
- 2018
-
Abstract
- Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, a marker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker, was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxide-induced DNA damage, showing a dose-dependent response. We observed a positive comet assay response to CM that was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and cat mRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induce apoptosis in retinal cells.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Caspase 3 metabolism
Cell Survival
Dose-Response Relationship, Drug
Histones metabolism
Hydrogen Peroxide adverse effects
Mutagenicity Tests
Photoreceptor Cells, Vertebrate drug effects
Photoreceptor Cells, Vertebrate metabolism
Zebrafish
DNA Damage
Oxidative Stress
Photoreceptor Cells, Vertebrate cytology
Pyrethrins toxicity
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3592
- Volume :
- 826
- Database :
- MEDLINE
- Journal :
- Mutation research. Genetic toxicology and environmental mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 29412866
- Full Text :
- https://doi.org/10.1016/j.mrgentox.2017.12.010