47 results on '"Paraskevopoulou S"'
Search Results
2. Readmission rates following Double Balloon Enteroscopy in patients with small intestinal bleeding and factors associated with re-hospitalization
- Author
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Stasinos, I., additional, Gongaki, S., additional, Voulgaris, T., additional, Paraskevopoulou, S., additional, Vlachou, E., additional, Kalantzis, C., additional, Tsibouris, P., additional, and Apostolopoulos, P., additional
- Published
- 2024
- Full Text
- View/download PDF
3. Spatio-temporal variability of benthic macrofauna in a coastal lagoon assessed by ecological interaction networks
- Author
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Paraskevopoulou, S., Monokrousos, N., Kappas, I., and Abatzopoulos, T. J.
- Published
- 2015
4. Mitochondrial genomes of the freshwater monogonont rotifer Brachionus fernandoi and of two additional B. calyciflorus sensu stricto lineages from Germany and the USA (Rotifera, Brachionidae)
- Author
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Kiemel, K., De Cahsan, B., Paraskevopoulou, S., Weithoff, G., Tiedemann, R., Kiemel, K., De Cahsan, B., Paraskevopoulou, S., Weithoff, G., and Tiedemann, R.
- Abstract
The Brachionus calyciflorus species complex was recently subdivided into four species, but genetic resources to resolve phylogenetic relationships within this complex are still lacking. We provide two complete mitochondrial (mt) genomes from B. calyciflorus sensu stricto (Germany, USA) and the mt coding sequences (cds) from a German B. fernandoi. Phylogenetic analysis placed our B. calyciflorus sensu stricto strains close to the published genomes of B. calyciflorus, forming the putative sister species to B. fernandoi. Global representatives of B. calyciflorus sensu stricto (i.e. Europe, USA, and China) are genetically closer related to each other than to B. fernandoi (average pairwise nucleotide diversity 0.079 intraspecific vs. 0.254 interspecific).
- Published
- 2022
5. Mitochondrial genomes of the freshwater monogonont rotifer Brachionus fernandoi and of two additional B. calyciflorus sensu stricto lineages from Germany and the USA (Rotifera, Brachionidae)
- Author
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Kiemel, K., primary, De Cahsan, B., additional, Paraskevopoulou, S., additional, Weithoff, G., additional, and Tiedemann, R., additional
- Published
- 2022
- Full Text
- View/download PDF
6. Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales
- Author
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Kuhn J.H., Adkins S., Agwanda B.R., Al Kubrusli R., Alkhovsky S.V., Amarasinghe G.K., Avsic-Zupanc T., Ayllon M.A., Bahl J., Balkema-Buschmann A., Ballinger M.J., Basler C.F., Bavari S., Beer M., Bejerman N., Bennett A.J., Bente D.A., Bergeron E., Bird B.H., Blair C.D., Blasdell K.R., Blystad D.-R., Bojko J., Borth W.B., Bradfute S., Breyta R., Briese T., Brown P.A., Brown J.K., Buchholz U.J., Buchmeier M.J., Bukreyev A., Burt F., Buttner C., Calisher C.H., Cao M., Casas I., Chandran K., Charrel R.N., Cheng Q., Chiaki Y., Chiapello M., Choi I.-R., Ciuffo M., Clegg J.C.S., Crozier I., Dal Bo E., de la Torre J.C., de Lamballerie X., de Swart R.L., Debat H., Dheilly N.M., Di Cicco E., Di Paola N., Di Serio F., Dietzgen R.G., Digiaro M., Dolnik O., Drebot M.A., Drexler J.F., Dundon W.G., Duprex W.P., Durrwald R., Dye J.M., Easton A.J., Ebihara H., Elbeaino T., Ergunay K., Ferguson H.W., Fooks A.R., Forgia M., Formenty P.B.H., Franova J., Freitas-Astua J., Fu J., Furl S., Gago-Zachert S., Gao G.F., Garcia M.L., Garcia-Sastre A., Garrison A.R., Gaskin T., Gonzalez J.-P.J., Griffiths A., Goldberg T.L., Groschup M.H., Gunther S., Hall R.A., Hammond J., Han T., Hepojoki J., Hewson R., Hong J., Hong N., Hongo S., Horie M., Hu J.S., Hu T., Hughes H.R., Huttner F., Hyndman T.H., Ilyas M., Jalkanen R., Jiang D., Jonson G.B., Junglen S., Kadono F., Kaukinen K.H., Kawate M., Klempa B., Klingstrom J., Kobinger G., Koloniuk I., Kondo H., Koonin E.V., Krupovic M., Kubota K., Kurath G., Laenen L., Lambert A.J., Langevin S.L., Lee B., Lefkowitz E.J., Leroy E.M., Li S., Li L., Li J., Liu H., Lukashevich I.S., Maes P., de Souza W.M., Marklewitz M., Marshall S.H., Marzano S.-Y.L., Massart S., McCauley J.W., Melzer M., Mielke-Ehret N., Miller K.M., Ming T.J., Mirazimi A., Mordecai G.J., Muhlbach H.-P., Muhlberger E., Naidu R., Natsuaki T., Navarro J.A., Netesov S.V., Neumann G., Nowotny N., Nunes M.R.T., Olmedo-Velarde A., Palacios G., Pallas V., Palyi B., Papa A., Paraskevopoulou S., Park A.C., Parrish C.R., Patterson D.A., Pauvolid-Correa A., Paweska J.T., Payne S., Peracchio C., Perez D.R., Postler T.S., Qi L., Radoshitzky S.R., Resende R.O., Reyes C.A., Rima B.K., Luna G.R., Romanowski V., Rota P., Rubbenstroth D., Rubino L., Runstadler J.A., Sabanadzovic S., Sall A.A., Salvato M.S., Sang R., Sasaya T., Schulze A.D., Schwemmle M., Shi M., Shi X., Shi Z., Shimomoto Y., Shirako Y., Siddell S.G., Simmonds P., Sironi M., Smagghe G., Smither S., Song J.-W., Spann K., Spengler J.R., Stenglein M.D., Stone D.M., Sugano J., Suttle C.A., Tabata A., Takada A., Takeuchi S., Tchouassi D.P., Teffer A., Tesh R.B., Thornburg N.J., Tomitaka Y., Tomonaga K., Tordo N., Torto B., Towner J.S., Tsuda S., Tu C., Turina M., Tzanetakis I.E., Uchida J., Usugi T., Vaira A.M., Vallino M., van den Hoogen B., Varsani A., Vasilakis N., Verbeek M., von Bargen S., Wada J., Wahl V., Walker P.J., Wang L.-F., Wang G., Wang Y., Waqas M., Wei T., Wen S., Whitfield A.E., Williams J.V., Wolf Y.I., Wu J., Xu L., Yanagisawa H., Yang C., Yang Z., Zerbini F.M., Zhai L., Zhang Y.-Z., Zhang S., Zhang J., Zhang Z., and Zhou X.
- Subjects
Virus classification ,Negative-stranded RNA viruses - Abstract
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratifcation vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/ or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV
- Published
- 2021
7. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales
- Author
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Kuhn, J.H., Adkins, S., Agwanda, B.R., Al Kubrusli, R., Alkhovsky, S.V., Amarasinghe, G.K., Avšič-Županc, T., Ayllón, M.A., Bahl, J., Balkema-Buschmann, A., Ballinger, M.J., Basler, C.F., Bavari, S., Beer, M., Bejerman, N., Bennett, A.J., Bente, D.A., Bergeron, É., Bird, B.H., Blair, C.D., Blasdell, K.R., Blystad, D-R, Bojko, J., Borth, W.B., Bradfute, S., Breyta, R., Briese, T., Brown, P.A., Brown, J.K., Buchholz, U.J., Buchmeier, M.J., Bukreyev, A., Burt, F., Büttner, C., Calisher, C.H., Cao, M., Casas, I., Chandran, K., Charrel, R.N., Cheng, Q., Chiaki, Y., Chiapello, M., Choi, I-R, Ciuffo, M., Clegg, J.C.S., Crozier, I., Dal Bó, E., de la Torre, J.C., de Lamballerie, X., de Swart, R.L., Debat, H., Dheilly, N.M., Di Cicco, E., Di Paola, N., Di Serio, F., Dietzgen, R.G., Digiaro, M., Dolnik, O., Drebot, M.A., Drexler, J.F., Dundon, W.G., Duprex, W.P., Dürrwald, R., Dye, J.M., Easton, A.J., Ebihara, H., Elbeaino, T., Ergünay, K., Ferguson, H.W., Fooks, A.R., Forgia, M., Formenty, P.B.H., Fránová, J., Freitas-Astúa, J., Fu, J., Fürl, S., Gago-Zachert, S., Gāo, G.F., García, M.L., García-Sastre, A., Garrison, A.R., Gaskin, T., Gonzalez, J-P.J., Griffiths, A., Goldberg, T.L., Groschup, M.H., Günther, S., Hall, R.A., Hammond, J., Han, T., Hepojoki, J., Hewson, R., Hong, J., Hong, N., Hongo, S., Horie, M., Hu, J.S., Hu, T., Hughes, H.R., Hüttner, F., Hyndman, T.H., Ilyas, M., Jalkanen, R., Jiāng, D., Jonson, G.B., Junglen, S., Kadono, F., Kaukinen, K.H., Kawate, M., Klempa, B., Klingström, J., Kobinger, G., Koloniuk, I., Kondo, H., Koonin, E.V., Krupovic, M., Kubota, K., Kurath, G., Laenen, L., Lambert, A.J., Langevin, S.L., Lee, B., Lefkowitz, E.J., Leroy, E.M., Li, S., Li, L., Lǐ, J., Liu, H., Lukashevich, I.S., Maes, P., de Souza, W.M., Marklewitz, M., Marshall, S.H., Marzano, S-Y.L., Massart, S., McCauley, J.W., Melzer, M., Mielke-Ehret, N., Miller, K.M., Ming, T.J., Mirazimi, A., Mordecai, G.J., Mühlbach, H-P, Mühlberger, E., Naidu, R., Natsuaki, T., Navarro, J.A., Netesov, S.V., Neumann, G., Nowotny, N., Nunes, M.R.T., Olmedo-Velarde, A., Palacios, G., Pallas, V., Pályi, B., Papa, A., Paraskevopoulou, S., Park, A.C., Parrish, C.R., Patterson, D.A., Pauvolid-Corrêa, A., Pawęska, J.T., Payne, S., Peracchio, C., Pérez, D.R., Postler, T.S., Qi, L., Radoshitzky, S.R., Resende, R.O., Reyes, C.A., Rima, B.K., Luna, G.R., Romanowski, V., Rota, P., Rubbenstroth, D., Rubino, L., Runstadler, J.A., Sabanadzovic, S., Sall, A.A., Salvato, M.S., Sang, R., Sasaya, T., Schulze, A.D., Schwemmle, M., Shi, M., Shi, X., Shí, Z., Shimomoto, Y., Shirako, Y., Siddell, S.G., Simmonds, P., Sironi, M., Smagghe, G., Smither, S., Song, J-W, Spann, K., Spengler, J.R., Stenglein, M.D., Stone, D.M., Sugano, J., Suttle, C.A., Tabata, A., Takada, A., Takeuchi, S., Tchouassi, D.P., Teffer, A., Tesh, R.B., Thornburg, N.J., Tomitaka, Y., Tomonaga, K., Tordo, N., Torto, B., Towner, J.S., Tsuda, S., Tu, C., Turina, M., Tzanetakis, I.E., Uchida, J., Usugi, T., Vaira, A.M., Vallino, M., van den Hoogen, B., Varsani, A., Vasilakis, N., Verbeek, M., von Bargen, S., Wada, J., Wahl, V., Walker, P.J., Wang, L-F, Wang, G., Wang, Y., Waqas, M., Wèi, T., Wen, S., Whitfield, A.E., Williams, J.V., Wolf, Y.I., Wu, J., Xu, L., Yanagisawa, H., Yang, C., Yang, Z., Zerbini, F.M., Zhai, L., Zhang, Y-Z, Zhang, S., Zhang, J., Zhang, Z., Zhou, X., Kuhn, J.H., Adkins, S., Agwanda, B.R., Al Kubrusli, R., Alkhovsky, S.V., Amarasinghe, G.K., Avšič-Županc, T., Ayllón, M.A., Bahl, J., Balkema-Buschmann, A., Ballinger, M.J., Basler, C.F., Bavari, S., Beer, M., Bejerman, N., Bennett, A.J., Bente, D.A., Bergeron, É., Bird, B.H., Blair, C.D., Blasdell, K.R., Blystad, D-R, Bojko, J., Borth, W.B., Bradfute, S., Breyta, R., Briese, T., Brown, P.A., Brown, J.K., Buchholz, U.J., Buchmeier, M.J., Bukreyev, A., Burt, F., Büttner, C., Calisher, C.H., Cao, M., Casas, I., Chandran, K., Charrel, R.N., Cheng, Q., Chiaki, Y., Chiapello, M., Choi, I-R, Ciuffo, M., Clegg, J.C.S., Crozier, I., Dal Bó, E., de la Torre, J.C., de Lamballerie, X., de Swart, R.L., Debat, H., Dheilly, N.M., Di Cicco, E., Di Paola, N., Di Serio, F., Dietzgen, R.G., Digiaro, M., Dolnik, O., Drebot, M.A., Drexler, J.F., Dundon, W.G., Duprex, W.P., Dürrwald, R., Dye, J.M., Easton, A.J., Ebihara, H., Elbeaino, T., Ergünay, K., Ferguson, H.W., Fooks, A.R., Forgia, M., Formenty, P.B.H., Fránová, J., Freitas-Astúa, J., Fu, J., Fürl, S., Gago-Zachert, S., Gāo, G.F., García, M.L., García-Sastre, A., Garrison, A.R., Gaskin, T., Gonzalez, J-P.J., Griffiths, A., Goldberg, T.L., Groschup, M.H., Günther, S., Hall, R.A., Hammond, J., Han, T., Hepojoki, J., Hewson, R., Hong, J., Hong, N., Hongo, S., Horie, M., Hu, J.S., Hu, T., Hughes, H.R., Hüttner, F., Hyndman, T.H., Ilyas, M., Jalkanen, R., Jiāng, D., Jonson, G.B., Junglen, S., Kadono, F., Kaukinen, K.H., Kawate, M., Klempa, B., Klingström, J., Kobinger, G., Koloniuk, I., Kondo, H., Koonin, E.V., Krupovic, M., Kubota, K., Kurath, G., Laenen, L., Lambert, A.J., Langevin, S.L., Lee, B., Lefkowitz, E.J., Leroy, E.M., Li, S., Li, L., Lǐ, J., Liu, H., Lukashevich, I.S., Maes, P., de Souza, W.M., Marklewitz, M., Marshall, S.H., Marzano, S-Y.L., Massart, S., McCauley, J.W., Melzer, M., Mielke-Ehret, N., Miller, K.M., Ming, T.J., Mirazimi, A., Mordecai, G.J., Mühlbach, H-P, Mühlberger, E., Naidu, R., Natsuaki, T., Navarro, J.A., Netesov, S.V., Neumann, G., Nowotny, N., Nunes, M.R.T., Olmedo-Velarde, A., Palacios, G., Pallas, V., Pályi, B., Papa, A., Paraskevopoulou, S., Park, A.C., Parrish, C.R., Patterson, D.A., Pauvolid-Corrêa, A., Pawęska, J.T., Payne, S., Peracchio, C., Pérez, D.R., Postler, T.S., Qi, L., Radoshitzky, S.R., Resende, R.O., Reyes, C.A., Rima, B.K., Luna, G.R., Romanowski, V., Rota, P., Rubbenstroth, D., Rubino, L., Runstadler, J.A., Sabanadzovic, S., Sall, A.A., Salvato, M.S., Sang, R., Sasaya, T., Schulze, A.D., Schwemmle, M., Shi, M., Shi, X., Shí, Z., Shimomoto, Y., Shirako, Y., Siddell, S.G., Simmonds, P., Sironi, M., Smagghe, G., Smither, S., Song, J-W, Spann, K., Spengler, J.R., Stenglein, M.D., Stone, D.M., Sugano, J., Suttle, C.A., Tabata, A., Takada, A., Takeuchi, S., Tchouassi, D.P., Teffer, A., Tesh, R.B., Thornburg, N.J., Tomitaka, Y., Tomonaga, K., Tordo, N., Torto, B., Towner, J.S., Tsuda, S., Tu, C., Turina, M., Tzanetakis, I.E., Uchida, J., Usugi, T., Vaira, A.M., Vallino, M., van den Hoogen, B., Varsani, A., Vasilakis, N., Verbeek, M., von Bargen, S., Wada, J., Wahl, V., Walker, P.J., Wang, L-F, Wang, G., Wang, Y., Waqas, M., Wèi, T., Wen, S., Whitfield, A.E., Williams, J.V., Wolf, Y.I., Wu, J., Xu, L., Yanagisawa, H., Yang, C., Yang, Z., Zerbini, F.M., Zhai, L., Zhang, Y-Z, Zhang, S., Zhang, J., Zhang, Z., and Zhou, X.
- Abstract
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
- Published
- 2021
8. Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales
- Author
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Kuhn, J.H., Adkins, S., Agwanda, B.R., Al Kubrusli, R., Alkhovsky, S.V., Amarasinghe, G.K., Avšič-Županc, T., Ayllón, M.A., Bahl, J., Balkema-Buschmann, A., Ballinger, M.J., Basler, C.F., Bavari, S., Beer, M., Bejerman, N., Bennett, A.J., Bente, D.A., Bergeron, É., Bird, B.H., Blair, C.D., Blasdell, K.R., Blystad, D-R, Bojko, J., Borth, W.B., Bradfute, S., Breyta, R., Briese, T., Brown, P.A., Brown, J.K., Buchholz, U.J., Buchmeier, M.J., Bukreyev, A., Burt, F., Büttner, C., Calisher, C.H., Cao, M., Casas, I., Chandran, K., Charrel, R.N., Cheng, Q., Chiaki, Y., Chiapello, M., Choi, I-R, Ciuffo, M., Clegg, J.C.S., Crozier, I., Dal Bó, E., de la Torre, J.C., de Lamballerie, X., de Swart, R.L., Debat, H., Dheilly, N.M., Di Cicco, E., Di Paola, N., Di Serio, F., Dietzgen, R.G., Digiaro, M., Dolnik, O., Drebot, M.A., Drexler, J.F., Dundon, W.G., Duprex, W.P., Dürrwald, R., Dye, J.M., Easton, A.J., Ebihara, H., Elbeaino, T., Ergünay, K., Ferguson, H.W., Fooks, A.R., Forgia, M., Formenty, P.B.H., Fránová, J., Freitas-Astúa, J., Fu, J., Fürl, S., Gago-Zachert, S., Gāo, G.F., García, M.L., García-Sastre, A., Garrison, A.R., Gaskin, T., Gonzalez, J-P.J., Griffiths, A., Goldberg, T.L., Groschup, M.H., Günther, S., Hall, R.A., Hammond, J., Han, T., Hepojoki, J., Hewson, R., Hong, J., Hong, N., Hongo, S., Horie, M., Hu, J.S., Hu, T., Hughes, H.R., Hüttner, F., Hyndman, T.H., Ilyas, M., Jalkanen, R., Jiāng, D., Jonson, G.B., Junglen, S., Kadono, F., Kaukinen, K.H., Kawate, M., Klempa, B., Klingström, J., Kobinger, G., Koloniuk, I., Kondo, H., Koonin, E.V., Krupovic, M., Kubota, K., Kurath, G., Laenen, L., Lambert, A.J., Langevin, S.L., Lee, B., Lefkowitz, E.J., Leroy, E.M., Li, S., Li, L., Lǐ, J., Liu, H., Lukashevich, I.S., Maes, P., de Souza, W.M., Marklewitz, M., Marshall, S.H., Marzano, S-Y.L., Massart, S., McCauley, J.W., Melzer, M., Mielke-Ehret, N., Miller, K.M., Ming, T.J., Mirazimi, A., Mordecai, G.J., Mühlbach, H-P, Mühlberger, E., Naidu, R., Natsuaki, T., Navarro, J.A., Netesov, S.V., Neumann, G., Nowotny, N., Nunes, M.R.T., Olmedo-Velarde, A., Palacios, G., Pallás, V., Pályi, B., Papa, A., Paraskevopoulou, S., Park, A.C., Parrish, C.R., Patterson, D.A., Pauvolid-Corrêa, A., Pawęska, J.T., Payne, S., Peracchio, C., Pérez, D.R., Postler, T.S., Qi, L., Radoshitzky, S.R., Resende, R.O., Reyes, C.A., Rima, B.K., Luna, G.R., Romanowski, V., Rota, P., Rubbenstroth, D., Rubino, L., Runstadler, J.A., Sabanadzovic, S., Sall, A.A., Salvato, M.S., Sang, R., Sasaya, T., Schulze, A.D., Schwemmle, M., Shi, M., Shí, X., Shí, Z., Shimomoto, Y., Shirako, Y., Siddell, S.G., Simmonds, P., Sironi, M., Smagghe, G., Smither, S., Song, J-W, Spann, K., Spengler, J.R., Stenglein, M.D., Stone, D.M., Sugano, J., Suttle, C.A., Tabata, A., Takada, A., Takeuchi, S., Tchouassi, D.P., Teffer, A., Tesh, R.B., Thornburg, N. J., Tomitaka, Y., Tomonaga, K., Tordo, N., Torto, B., Towner, J.S., Tsuda, S., Tu, C., Turina, M., Tzanetakis, I.E., Uchida, J., Usugi, T., Vaira, A.M., Vallino, M., van den Hoogen, B., Varsani, A., Vasilakis, N., Verbeek, M., von Bargen, S., Wada, J., Wahl, V., Walker, P.J., Wang, L-F, Wang, G., Wang, Y., Waqas, M., Wèi, T., Wen, S., Whitfield, A.E., Williams, J.V., Wolf, Y.I., Wu, J., Xu, L., Yanagisawa, H., Yang, C., Yang, Z., Zerbini, F.M., Zhai, L., Zhang, Y-Z, Zhang, S., Zhang, J., Zhang, Z., Zhou, X., Kuhn, J.H., Adkins, S., Agwanda, B.R., Al Kubrusli, R., Alkhovsky, S.V., Amarasinghe, G.K., Avšič-Županc, T., Ayllón, M.A., Bahl, J., Balkema-Buschmann, A., Ballinger, M.J., Basler, C.F., Bavari, S., Beer, M., Bejerman, N., Bennett, A.J., Bente, D.A., Bergeron, É., Bird, B.H., Blair, C.D., Blasdell, K.R., Blystad, D-R, Bojko, J., Borth, W.B., Bradfute, S., Breyta, R., Briese, T., Brown, P.A., Brown, J.K., Buchholz, U.J., Buchmeier, M.J., Bukreyev, A., Burt, F., Büttner, C., Calisher, C.H., Cao, M., Casas, I., Chandran, K., Charrel, R.N., Cheng, Q., Chiaki, Y., Chiapello, M., Choi, I-R, Ciuffo, M., Clegg, J.C.S., Crozier, I., Dal Bó, E., de la Torre, J.C., de Lamballerie, X., de Swart, R.L., Debat, H., Dheilly, N.M., Di Cicco, E., Di Paola, N., Di Serio, F., Dietzgen, R.G., Digiaro, M., Dolnik, O., Drebot, M.A., Drexler, J.F., Dundon, W.G., Duprex, W.P., Dürrwald, R., Dye, J.M., Easton, A.J., Ebihara, H., Elbeaino, T., Ergünay, K., Ferguson, H.W., Fooks, A.R., Forgia, M., Formenty, P.B.H., Fránová, J., Freitas-Astúa, J., Fu, J., Fürl, S., Gago-Zachert, S., Gāo, G.F., García, M.L., García-Sastre, A., Garrison, A.R., Gaskin, T., Gonzalez, J-P.J., Griffiths, A., Goldberg, T.L., Groschup, M.H., Günther, S., Hall, R.A., Hammond, J., Han, T., Hepojoki, J., Hewson, R., Hong, J., Hong, N., Hongo, S., Horie, M., Hu, J.S., Hu, T., Hughes, H.R., Hüttner, F., Hyndman, T.H., Ilyas, M., Jalkanen, R., Jiāng, D., Jonson, G.B., Junglen, S., Kadono, F., Kaukinen, K.H., Kawate, M., Klempa, B., Klingström, J., Kobinger, G., Koloniuk, I., Kondo, H., Koonin, E.V., Krupovic, M., Kubota, K., Kurath, G., Laenen, L., Lambert, A.J., Langevin, S.L., Lee, B., Lefkowitz, E.J., Leroy, E.M., Li, S., Li, L., Lǐ, J., Liu, H., Lukashevich, I.S., Maes, P., de Souza, W.M., Marklewitz, M., Marshall, S.H., Marzano, S-Y.L., Massart, S., McCauley, J.W., Melzer, M., Mielke-Ehret, N., Miller, K.M., Ming, T.J., Mirazimi, A., Mordecai, G.J., Mühlbach, H-P, Mühlberger, E., Naidu, R., Natsuaki, T., Navarro, J.A., Netesov, S.V., Neumann, G., Nowotny, N., Nunes, M.R.T., Olmedo-Velarde, A., Palacios, G., Pallás, V., Pályi, B., Papa, A., Paraskevopoulou, S., Park, A.C., Parrish, C.R., Patterson, D.A., Pauvolid-Corrêa, A., Pawęska, J.T., Payne, S., Peracchio, C., Pérez, D.R., Postler, T.S., Qi, L., Radoshitzky, S.R., Resende, R.O., Reyes, C.A., Rima, B.K., Luna, G.R., Romanowski, V., Rota, P., Rubbenstroth, D., Rubino, L., Runstadler, J.A., Sabanadzovic, S., Sall, A.A., Salvato, M.S., Sang, R., Sasaya, T., Schulze, A.D., Schwemmle, M., Shi, M., Shí, X., Shí, Z., Shimomoto, Y., Shirako, Y., Siddell, S.G., Simmonds, P., Sironi, M., Smagghe, G., Smither, S., Song, J-W, Spann, K., Spengler, J.R., Stenglein, M.D., Stone, D.M., Sugano, J., Suttle, C.A., Tabata, A., Takada, A., Takeuchi, S., Tchouassi, D.P., Teffer, A., Tesh, R.B., Thornburg, N. J., Tomitaka, Y., Tomonaga, K., Tordo, N., Torto, B., Towner, J.S., Tsuda, S., Tu, C., Turina, M., Tzanetakis, I.E., Uchida, J., Usugi, T., Vaira, A.M., Vallino, M., van den Hoogen, B., Varsani, A., Vasilakis, N., Verbeek, M., von Bargen, S., Wada, J., Wahl, V., Walker, P.J., Wang, L-F, Wang, G., Wang, Y., Waqas, M., Wèi, T., Wen, S., Whitfield, A.E., Williams, J.V., Wolf, Y.I., Wu, J., Xu, L., Yanagisawa, H., Yang, C., Yang, Z., Zerbini, F.M., Zhai, L., Zhang, Y-Z, Zhang, S., Zhang, J., Zhang, Z., and Zhou, X.
- Abstract
Unfortunately, the inclusion of original names (in non-Latin script) of the following authors caused problems with author name indexing in PubMed. Therefore, these original names were removed from XML data to correct the PubMed record...
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- 2021
9. The International Virus Bioinformatics Meeting 2020
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Hufsky, F. (Franziska), Beerenwinkel, N. (Niko), Meyer, I.M. (Irmtraud M.), Roux, S. (Simon), Cook, G.M. (Georgia May), Kinsella, C.M. (Cormac M.), Lamkiewicz, K. (Kevin), Marquet, M. (Mike), Nieuwenhuijse, D.F. (David F.), Olendraite, I. (Ingrida), Paraskevopoulou, S. (Sofia), Young, F. (Francesca), Dijkman, R. (Ronald), Ibrahim, B. (Bashar), Kelly, J. (Jenna), Le Mercier, P. (Philippe), Marz, M. (Manja), Ramette, A. (Alban), Thiel, V. (Volker), Hufsky, F. (Franziska), Beerenwinkel, N. (Niko), Meyer, I.M. (Irmtraud M.), Roux, S. (Simon), Cook, G.M. (Georgia May), Kinsella, C.M. (Cormac M.), Lamkiewicz, K. (Kevin), Marquet, M. (Mike), Nieuwenhuijse, D.F. (David F.), Olendraite, I. (Ingrida), Paraskevopoulou, S. (Sofia), Young, F. (Francesca), Dijkman, R. (Ronald), Ibrahim, B. (Bashar), Kelly, J. (Jenna), Le Mercier, P. (Philippe), Marz, M. (Manja), Ramette, A. (Alban), and Thiel, V. (Volker)
- Abstract
The International Virus Bioinformatics Meeting 2020 was originally planned to take place in Bern, Switzerland, in March 2020. However, the COVID-19 pandemic put a spoke in the wheel of almost all conferences to be held in 2020. After moving the conference to 8-9 October 2020, we got hit by the second wave and finally decided at short notice to go fully online. On the other hand, the pandemic has made us even more aware of the importance of accelerating research in viral bioinformatics. Advances in bioinformatics have led to improved approaches to investigate viral infections and outbreaks. The International Virus Bioinformatics Meeting 2020 has attracted approximately 120 experts in virology and bioinformatics from all over the world to join the two-day virtual meeting. Despite concerns being raised that virtual meetings lack possibilities for face-to-face discussion, the participants from this small community created a highly interactive scientific environment, engaging in lively and inspiring discussions and suggesting new research directions and questions. The meeting featured five invited and twelve contributed talks, on the four main topics: (1) proteome and RNAome of R
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- 2020
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10. The International Virus Bioinformatics Meeting 2020
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Hufsky, F, Beerenwinkel, N, Meyer, IM, Roux, S, Cook, GM, Kinsella, CM, Lamkiewicz, K, Marquet, M, Nieuwenhuijse, David, Olendraite, I, Paraskevopoulou, S, Young, F, Dijkman, R, Ibrahim, B, Kelly, J, Le Mercier, P, Marz, M, Ramette, A, Thiel, V, Hufsky, F, Beerenwinkel, N, Meyer, IM, Roux, S, Cook, GM, Kinsella, CM, Lamkiewicz, K, Marquet, M, Nieuwenhuijse, David, Olendraite, I, Paraskevopoulou, S, Young, F, Dijkman, R, Ibrahim, B, Kelly, J, Le Mercier, P, Marz, M, Ramette, A, and Thiel, V
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- 2020
11. EDUCATIONAL NEEDS AND SYNDROME OF OCCUPATIONAL BURNOUT IN ADMINISTRATIVE STAFF OF HEALTHCARE UNITS.
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PARASKEVOPOULOU, S., SIAMETI, M., and TZANAKIS, E.
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PSYCHOLOGICAL burnout ,MEDICAL personnel ,PERSONNEL management ,ORGANIZATIONAL effectiveness ,MEDICAL care - Abstract
Occupational burnout is particularly common in healthcare staff and is closely related to the quality of services provided. Most relevant researches mainly focus on health professionals, bypassing the administrative staff of healthcare units. However, administrative staff plays a key role for the efficiency of healthcare services, and therefore the occupational burnout of administrative staff negatively affects the organization and quality of the provided healthcare services, as patients may not receive proper care. The occupational burnout of administrative staff is a crucial and important issue and is worthy of study, because the new knowledge that will be acquired can be used in the design of training interventions regarding prevention and treatment. The purpose of this study is to investigate the phenomenon of occupational burnout of administrative staff in healthcare units with emphasis on the experience and needs, especially educational needs, of participants concerning the prevention and treatment of the problem. A qualitative methodology was used with semi-structured interviews with the administrative staff of Ippokrateio General Hospital of Thessaloniki. The main issues that emerged from the analysis of the interviews are: "fatigue" (physical and mental) and "working conditions" (organizational issues, communication) which are related to the experience, but also to the causes of occupational burnout, "work efficiency" related to the consequences of occupational burnout. The topic of "educational needs" is present in most of the participants' responses, but is most prominent in those that mention the organizational and management needs of employees, both individually and collectively, as the dominant factor in addressing the problem of occupational burnout. [ABSTRACT FROM AUTHOR]
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- 2020
12. OCCUPATIONAL BURNOUT, MENTAL HEALTH AND LIFE QUALITY OF NURSING STAFF IN HEALTH STRUCTURES IN GREECE: THE CASE OF IPPOKRATEIO GENERAL HOSPITAL OF THESSALONIKI.
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Paraskevopoulou, S., Siameti, M., and Tzanakis, E.
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MEDICAL personnel ,PSYCHOLOGICAL burnout ,QUALITY of life ,MENTAL health ,NIGHT work ,BEHAVIORAL sciences - Published
- 2020
13. A sub-1μW Neural Spike-Peak Detection and Spike-Count Rate Encoding Circuit
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Paraskevopoulou, S and Constandinou, TG
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Hardware_PERFORMANCEANDRELIABILITY ,Hardware_LOGICDESIGN - Abstract
In this paper we present a circuit for determining neural spike features such as peak occurrence, peak amplitude and spike count rate in continuous-time. The system achieves these functions concurrently and in real-time achieving an accuracy higher than a typical digital solution (constrained by a the sampling time and/or resolution). For an average spike rate of 50$spikes/s$ the system consumes 815nW designed in a commercially-available 0.18μm CMOS technology. The complete circuit core (excluding bondpads) occupies a total area of approximately 0.022mm² Accepted version
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- 2011
14. Towards Next Generation Neural Interfaces: Optimizing Power, Bandwidth and Data Quality
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Eftekhar, A, Paraskevopoulou, S, and Constandinou, TG
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In this paper, we review the state-of-the-art in neural interface recording architectures. Through this we identify schemes which show the trade-off between data information quality (lossiness), computation (i.e. power and area requirements) and the number of channels. These trade-offs are then extended by considering the front-end amplifier bandwidth to also be a variable. We therefore explore the possibility of band-limiting the spectral content of recorded neural signals (to save power) and investigate the effect this has on subsequent processing (spike detection accuracy). We identify the spike detection method most robust to such signals, optimize the threshold levels and modify this to exploit such a strategy. Accepted version
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- 2010
15. Measurements and modelling of a microcellular advanced mobile system.
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Papadakis, N., Paraskevopoulou, S., Theodosiadis, K., and Constantinou, P.
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- 1993
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16. CIEVaD: A Lightweight Workflow Collection for the Rapid and On-Demand Deployment of End-to-End Testing for Genomic Variant Detection.
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Krannich T, Ternovoj D, Paraskevopoulou S, and Fuchs S
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- High-Throughput Nucleotide Sequencing methods, Humans, Computational Biology methods, Workflow, Genomics methods, Genetic Variation, Software
- Abstract
The identification of genomic variants has become a routine task in the age of genome sequencing. In particular, small genomic variants of a single or few nucleotides are routinely investigated for their impact on an organism's phenotype. Hence, the precise and robust detection of the variants' exact genomic locations and changes in nucleotide composition is vital in many biological applications. Although a plethora of methods exist for the many key steps of variant detection, thoroughly testing the detection process and evaluating its results is still a cumbersome procedure. In this work, we present a collection of easy-to-apply and highly modifiable workflows to facilitate the generation of synthetic test data, as well as to evaluate the accordance of a user-provided set of variants with the test data. The workflows are implemented in Nextflow and are open-source and freely available on Github under the GPL-3.0 license.
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- 2024
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17. Author Correction: Immunogenetic-pathogen networks shrink in Tome's spiny rat, a generalist rodent inhabiting disturbed landscapes.
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Fleischer R, Eibner GJ, Schwensow NI, Pirzer F, Paraskevopoulou S, Mayer G, Corman VM, Drosten C, Wilhelm K, Heni AC, Sommer S, and Schmid DW
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- 2024
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18. Bus Riding as Amplification Mechanism for SARS-CoV-2 Transmission, Germany, 2021 1 .
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Schöll M, Höhn C, Boucsein J, Moek F, Plath J, An der Heiden M, Huska M, Kröger S, Paraskevopoulou S, Siffczyk C, Buchholz U, and Lachmann R
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- Child, Humans, Cohort Studies, Disease Outbreaks, Germany epidemiology, SARS-CoV-2, COVID-19 epidemiology
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To examine the risk associated with bus riding and identify transmission chains, we investigated a COVID-19 outbreak in Germany in 2021 that involved index case-patients among bus-riding students. We used routine surveillance data, performed laboratory analyses, interviewed case-patients, and conducted a cohort study. We identified 191 case-patients, 65 (34%) of whom were elementary schoolchildren. A phylogenetically unique strain and epidemiologic analyses provided a link between air travelers and cases among bus company staff, schoolchildren, other bus passengers, and their respective household members. The attack rate among bus-riding children at 1 school was ≈4 times higher than among children not taking a bus to that school. The outbreak exemplifies how an airborne agent may be transmitted effectively through (multiple) short (<20 minutes) public transport journeys and may rapidly affect many persons.
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- 2024
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19. Immunogenetic-pathogen networks shrink in Tome's spiny rat, a generalist rodent inhabiting disturbed landscapes.
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Fleischer R, Eibner GJ, Schwensow NI, Pirzer F, Paraskevopoulou S, Mayer G, Corman VM, Drosten C, Wilhelm K, Heni AC, Sommer S, and Schmid DW
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- Animals, Rats, Immunogenetics, Forests, Zoonoses, Rodentia genetics, Nematoda
- Abstract
Anthropogenic disturbance may increase the emergence of zoonoses. Especially generalists that cope with disturbance and live in close contact with humans and livestock may become reservoirs of zoonotic pathogens. Yet, whether anthropogenic disturbance modifies host-pathogen co-evolutionary relationships in generalists is unknown. We assessed pathogen diversity, neutral genome-wide diversity (SNPs) and adaptive MHC class II diversity in a rodent generalist inhabiting three lowland rainforest landscapes with varying anthropogenic disturbance, and determined which MHC alleles co-occurred more frequently with 13 gastrointestinal nematodes, blood trypanosomes, and four viruses. Pathogen-specific selection pressures varied between landscapes. Genome-wide diversity declined with the degree of disturbance, while MHC diversity was only reduced in the most disturbed landscape. Furthermore, pristine forest landscapes had more functional important MHC-pathogen associations when compared to disturbed forests. We show co-evolutionary links between host and pathogens impoverished in human-disturbed landscapes. This underscores that parasite-mediated selection might change even in generalist species following human disturbance which in turn may facilitate host switching and the emergence of zoonoses., (© 2024. The Author(s).)
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- 2024
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20. ICTV Virus Taxonomy Profile: Kolmioviridae 2024.
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Kuhn JH, Babaian A, Bergner LM, Dény P, Glebe D, Horie M, Koonin EV, Krupovic M, Paraskevopoulou S, de la Peña M, Smura T, and Hepojoki J
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- Animals, Humans, Biological Evolution, Negative-Sense RNA Viruses, RNA Polymerase II, Mammals, Helper Viruses, Viroids
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Kolmioviridae is a family for negative-sense RNA viruses with circular, viroid-like genomes of about 1.5-1.7 kb that are maintained in mammals, amphibians, birds, fish, insects and reptiles. Deltaviruses, for instance, can cause severe hepatitis in humans. Kolmiovirids encode delta antigen (DAg) and replicate using host-cell DNA-directed RNA polymerase II and ribozymes encoded in their genome and antigenome. They require evolutionary unrelated helper viruses to provide envelopes and incorporate helper virus proteins for infectious particle formation. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Kolmioviridae , which is available at ictv.global/report/kolmioviridae.
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- 2024
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21. Neighbourhood watch: genomic epidemiology of SARS-CoV-2 variants circulating in a German federal state, Mecklenburg-Western Pomerania, in 2020-2022.
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Kohler C, King J, Stacker L, Goller KV, Moritz J, Pohlmann A, Nath N, Tzvetkova A, Rieck M, Paraskevopoulou S, Beslic D, Hölzer M, Fuchs S, Ziemann J, Kaderali L, Beer M, Hübner NO, and Becker K
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- Humans, Pandemics, Phylogeny, Communicable Disease Control, Genomics, SARS-CoV-2 genetics, COVID-19 epidemiology
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ABSTRACT Global and even national genome surveillance approaches do not provide the resolution necessary for rapid and accurate direct response by local public health authorities. Hence, a regional network of microbiological laboratories in collaboration with the health departments of all districts of the German federal state of Mecklenburg-Western Pomerania (M-V) was formed to investigate the regional molecular epidemiology of circulating SARS-CoV-2 lineages between 11/2020 and 03/2022. More than 4750 samples from all M-V counties were sequenced using Illumina and Nanopore technologies. Overall, 3493 (73.5%) sequences fulfilled quality criteria for time-resolved and/or spatially-resolved maximum likelihood phylogenic analyses and k-mean/ median clustering (KMC). We identified 116 different Pangolin virus lineages that can be assigned to 16 Nextstrain clades. The ten most frequently detected virus lineages belonged to B.1.1.7, AY.122, AY.43, BA.1, B.1.617.2, BA.1.1, AY.9.2, AY.4, P.1 and AY.126. Time-resolved phylogenetic analyses showed the occurrence of virus clades as determined worldwide, but with a substantial delay of one to two months. Further spatio-temporal phylogenetic analyses revealed a regional outbreak of a Gamma variant limited to western M-V counties. Finally, KMC elucidated a successive introduction of the various virus lineages into M-V, possibly triggered by vacation periods with increased (inter-) national travel activities. The COVID-19 pandemic in M-V was shaped by a combination of several SARS-CoV-2 introductions, lockdown measures, restrictive quarantine of patients and the lineage specific replication rate. Complementing global and national surveillance, regional surveillance adds value by providing a higher level of surveillance resolution tailored to local health authorities.
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- 2023
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22. ICTV Virus Taxonomy Profile: Jingchuvirales 2023.
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Kuhn JH, Dheilly NM, Junglen S, Paraskevopoulou S, Shi M, and Di Paola N
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- Humans, Animals, Phylogeny, Nucleoproteins genetics, Negative-Sense RNA Viruses, Virus Replication, Virion, Genome, Viral, RNA Viruses genetics
- Abstract
Jingchuvirales is an order of negative-sense RNA viruses with genomes of 9.1-15.3 kb that have been associated with arachnids, barnacles, crustaceans, insects, fish and reptiles in Africa, Asia, Australia, Europe, North America and South America. The jingchuviral genome has two to four open reading frames (ORFs) that encode a glycoprotein (GP), a nucleoprotein (NP), a large (L) protein containing an RNA-directed RNA polymerase (RdRP) domain, and/or proteins of unknown function. Viruses in the order are only known from their genome sequences. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the order Jingchuvirales and on the families Aliusviridae , Chuviridae , Crepuscuviridae , Myriaviridae and Natareviridae , which are available at ictv.global/report/jingchuvirales, ictv.global/report/aliusviridae, ictv.global/report/chuviridae, ictv.global/report/crepuscuviridae, ictv.global/report/myriaviridae and ictv.global/report/natareviridae, respectively.
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- 2023
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23. ICTV Virus Taxonomy Profile: Xinmoviridae 2023.
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Sharpe S and Paraskevopoulou S
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- Host Specificity, RNA
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Xinmoviridae is a family of viruses with negative-sense RNA genomes of 9-14 kilobases. Xinmovirids typically infect beneficial and pest insects but their host range has not yet been investigated systematically and hence may be broader. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family of Xinmoviridae , which is available at ictv.global/report/xinmoviridae.
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- 2023
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24. Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria : kingdom Orthornavirae : phylum Negarnaviricota ).
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Kuhn JH, Abe J, Adkins S, Alkhovsky SV, Avšič-Županc T, Ayllón MA, Bahl J, Balkema-Buschmann A, Ballinger MJ, Kumar Baranwal V, Beer M, Bejerman N, Bergeron É, Biedenkopf N, Blair CD, Blasdell KR, Blouin AG, Bradfute SB, Briese T, Brown PA, Buchholz UJ, Buchmeier MJ, Bukreyev A, Burt F, Büttner C, Calisher CH, Cao M, Casas I, Chandran K, Charrel RN, Kumar Chaturvedi K, Chooi KM, Crane A, Dal Bó E, Carlos de la Torre J, de Souza WM, de Swart RL, Debat H, Dheilly NM, Di Paola N, Di Serio F, Dietzgen RG, Digiaro M, Drexler JF, Duprex WP, Dürrwald R, Easton AJ, Elbeaino T, Ergünay K, Feng G, Firth AE, Fooks AR, Formenty PBH, Freitas-Astúa J, Gago-Zachert S, Laura García M, García-Sastre A, Garrison AR, Gaskin TR, Gong W, Gonzalez JJ, de Bellocq J, Griffiths A, Groschup MH, Günther I, Günther S, Hammond J, Hasegawa Y, Hayashi K, Hepojoki J, Higgins CM, Hongō S, Horie M, Hughes HR, Hume AJ, Hyndman TH, Ikeda K, Jiāng D, Jonson GB, Junglen S, Klempa B, Klingström J, Kondō H, Koonin EV, Krupovic M, Kubota K, Kurath G, Laenen L, Lambert AJ, Lǐ J, Li JM, Liu R, Lukashevich IS, MacDiarmid RM, Maes P, Marklewitz M, Marshall SH, Marzano SL, McCauley JW, Mirazimi A, Mühlberger E, Nabeshima T, Naidu R, Natsuaki T, Navarro B, Navarro JA, Neriya Y, Netesov SV, Neumann G, Nowotny N, Nunes MRT, Ochoa-Corona FM, Okada T, Palacios G, Pallás V, Papa A, Paraskevopoulou S, Parrish CR, Pauvolid-Corrêa A, Pawęska JT, Pérez DR, Pfaff F, Plemper RK, Postler TS, Rabbidge LO, Radoshitzky SR, Ramos-González PL, Rehanek M, Resende RO, Reyes CA, Rodrigues TCS, Romanowski V, Rubbenstroth D, Rubino L, Runstadler JA, Sabanadzovic S, Sadiq S, Salvato MS, Sasaya T, Schwemmle M, Sharpe SR, Shi M, Shimomoto Y, Kavi Sidharthan V, Sironi M, Smither S, Song JW, Spann KM, Spengler JR, Stenglein MD, Takada A, Takeyama S, Tatara A, Tesh RB, Thornburg NJ, Tian X, Tischler ND, Tomitaka Y, Tomonaga K, Tordo N, Tu C, Turina M, Tzanetakis IE, Maria Vaira A, van den Hoogen B, Vanmechelen B, Vasilakis N, Verbeek M, von Bargen S, Wada J, Wahl V, Walker PJ, Waltzek TB, Whitfield AE, Wolf YI, Xia H, Xylogianni E, Yanagisawa H, Yano K, Ye G, Yuan Z, Zerbini FM, Zhang G, Zhang S, Zhang YZ, Zhao L, and Økland AL
- Subjects
- RNA-Dependent RNA Polymerase genetics, Negative-Sense RNA Viruses, RNA Viruses genetics
- Abstract
In April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
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- 2023
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25. ICTV Virus Taxonomy Profile: Lispiviridae 2023.
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Li JM, Wang F, Ye G, and Paraskevopoulou S
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- Animals, Nucleoproteins, Open Reading Frames, RNA, RNA-Dependent RNA Polymerase, Arthropods
- Abstract
Members of the family Lispiviridae are viruses with negative-sense RNA genomes of 6.5-15.5 kb that have mainly been found in arthropods and nematodes. The genomes of lispivirids contain several open reading frames, typically encoding a nucleoprotein (N), a glycoprotein (G), and a large protein (L) including an RNA-directed RNA polymerase (RdRP) domain. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Lispiviridae , which is available at ictv.global/report/lispiviridae.
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- 2023
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26. 2022 taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.
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Kuhn JH, Adkins S, Alkhovsky SV, Avšič-Županc T, Ayllón MA, Bahl J, Balkema-Buschmann A, Ballinger MJ, Bandte M, Beer M, Bejerman N, Bergeron É, Biedenkopf N, Bigarré L, Blair CD, Blasdell KR, Bradfute SB, Briese T, Brown PA, Bruggmann R, Buchholz UJ, Buchmeier MJ, Bukreyev A, Burt F, Büttner C, Calisher CH, Candresse T, Carson J, Casas I, Chandran K, Charrel RN, Chiaki Y, Crane A, Crane M, Dacheux L, Bó ED, de la Torre JC, de Lamballerie X, de Souza WM, de Swart RL, Dheilly NM, Di Paola N, Di Serio F, Dietzgen RG, Digiaro M, Drexler JF, Duprex WP, Dürrwald R, Easton AJ, Elbeaino T, Ergünay K, Feng G, Feuvrier C, Firth AE, Fooks AR, Formenty PBH, Freitas-Astúa J, Gago-Zachert S, García ML, García-Sastre A, Garrison AR, Godwin SE, Gonzalez JJ, de Bellocq JG, Griffiths A, Groschup MH, Günther S, Hammond J, Hepojoki J, Hierweger MM, Hongō S, Horie M, Horikawa H, Hughes HR, Hume AJ, Hyndman TH, Jiāng D, Jonson GB, Junglen S, Kadono F, Karlin DG, Klempa B, Klingström J, Koch MC, Kondō H, Koonin EV, Krásová J, Krupovic M, Kubota K, Kuzmin IV, Laenen L, Lambert AJ, Lǐ J, Li JM, Lieffrig F, Lukashevich IS, Luo D, Maes P, Marklewitz M, Marshall SH, Marzano SL, McCauley JW, Mirazimi A, Mohr PG, Moody NJG, Morita Y, Morrison RN, Mühlberger E, Naidu R, Natsuaki T, Navarro JA, Neriya Y, Netesov SV, Neumann G, Nowotny N, Ochoa-Corona FM, Palacios G, Pallandre L, Pallás V, Papa A, Paraskevopoulou S, Parrish CR, Pauvolid-Corrêa A, Pawęska JT, Pérez DR, Pfaff F, Plemper RK, Postler TS, Pozet F, Radoshitzky SR, Ramos-González PL, Rehanek M, Resende RO, Reyes CA, Romanowski V, Rubbenstroth D, Rubino L, Rumbou A, Runstadler JA, Rupp M, Sabanadzovic S, Sasaya T, Schmidt-Posthaus H, Schwemmle M, Seuberlich T, Sharpe SR, Shi M, Sironi M, Smither S, Song JW, Spann KM, Spengler JR, Stenglein MD, Takada A, Tesh RB, Těšíková J, Thornburg NJ, Tischler ND, Tomitaka Y, Tomonaga K, Tordo N, Tsunekawa K, Turina M, Tzanetakis IE, Vaira AM, van den Hoogen B, Vanmechelen B, Vasilakis N, Verbeek M, von Bargen S, Wada J, Wahl V, Walker PJ, Whitfield AE, Williams JV, Wolf YI, Yamasaki J, Yanagisawa H, Ye G, Zhang YZ, and Økland AL
- Subjects
- Humans, Phylogeny, Mononegavirales genetics, Viruses
- Abstract
In March 2022, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by two new families (bunyaviral Discoviridae and Tulasviridae), 41 new genera, and 98 new species. Three hundred forty-nine species were renamed and/or moved. The accidentally misspelled names of seven species were corrected. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV., (© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
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- 2022
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27. Advancing Precision Vaccinology by Molecular and Genomic Surveillance of Severe Acute Respiratory Syndrome Coronavirus 2 in Germany, 2021.
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Oh DY, Hölzer M, Paraskevopoulou S, Trofimova M, Hartkopf F, Budt M, Wedde M, Richard H, Haldemann B, Domaszewska T, Reiche J, Keeren K, Radonić A, Ramos Calderón JP, Smith MR, Brinkmann A, Trappe K, Drechsel O, Klaper K, Hein S, Hildt E, Haas W, Calvignac-Spencer S, Semmler T, Dürrwald R, Thürmer A, Drosten C, Fuchs S, Kröger S, von Kleist M, and Wolff T
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- Genome, Viral, Genomics, Humans, Phylogeny, Vaccinology, COVID-19 epidemiology, COVID-19 prevention & control, SARS-CoV-2 genetics
- Abstract
Background: Comprehensive pathogen genomic surveillance represents a powerful tool to complement and advance precision vaccinology. The emergence of the Alpha variant in December 2020 and the resulting efforts to track the spread of this and other severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern led to an expansion of genomic sequencing activities in Germany., Methods: At Robert Koch Institute (RKI), the German National Institute of Public Health, we established the Integrated Molecular Surveillance for SARS-CoV-2 (IMS-SC2) network to perform SARS-CoV-2 genomic surveillance at the national scale, SARS-CoV-2-positive samples from laboratories distributed across Germany regularly undergo whole-genome sequencing at RKI., Results: We report analyses of 3623 SARS-CoV-2 genomes collected between December 2020 and December 2021, of which 3282 were randomly sampled. All variants of concern were identified in the sequenced sample set, at ratios equivalent to those in the 100-fold larger German GISAID sequence dataset from the same time period. Phylogenetic analysis confirmed variant assignments. Multiple mutations of concern emerged during the observation period. To model vaccine effectiveness in vitro, we employed authentic-virus neutralization assays, confirming that both the Beta and Zeta variants are capable of immune evasion. The IMS-SC2 sequence dataset facilitated an estimate of the SARS-CoV-2 incidence based on genetic evolution rates. Together with modeled vaccine efficacies, Delta-specific incidence estimation indicated that the German vaccination campaign contributed substantially to a deceleration of the nascent German Delta wave., Conclusions: SARS-CoV-2 molecular and genomic surveillance may inform public health policies including vaccination strategies and enable a proactive approach to controlling coronavirus disease 2019 spread as the virus evolves., Competing Interests: Potential conflicts of interest. The authors report no potential conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2022. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.)
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- 2022
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28. Women in the European Virus Bioinformatics Center.
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Hufsky F, Abecasis A, Agudelo-Romero P, Bletsa M, Brown K, Claus C, Deinhardt-Emmer S, Deng L, Friedel CC, Gismondi MI, Kostaki EG, Kühnert D, Kulkarni-Kale U, Metzner KJ, Meyer IM, Miozzi L, Nishimura L, Paraskevopoulou S, Pérez-Cataluña A, Rahlff J, Thomson E, Tumescheit C, van der Hoek L, Van Espen L, Vandamme AM, Zaheri M, Zuckerman N, and Marz M
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- Europe, Female, Humans, Computational Biology, Research Personnel statistics & numerical data, Viruses genetics
- Abstract
Viruses are the cause of a considerable burden to human, animal and plant health, while on the other hand playing an important role in regulating entire ecosystems. The power of new sequencing technologies combined with new tools for processing "Big Data" offers unprecedented opportunities to answer fundamental questions in virology. Virologists have an urgent need for virus-specific bioinformatics tools. These developments have led to the formation of the European Virus Bioinformatics Center, a network of experts in virology and bioinformatics who are joining forces to enable extensive exchange and collaboration between these research areas. The EVBC strives to provide talented researchers with a supportive environment free of gender bias, but the gender gap in science, especially in math-intensive fields such as computer science, persists. To bring more talented women into research and keep them there, we need to highlight role models to spark their interest, and we need to ensure that female scientists are not kept at lower levels but are given the opportunity to lead the field. Here we showcase the work of the EVBC and highlight the achievements of some outstanding women experts in virology and viral bioinformatics.
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- 2022
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29. History and classification of Aigai virus (formerly Crimean-Congo haemorrhagic fever virus genotype VI).
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Papa A, Marklewitz M, Paraskevopoulou S, Garrison AR, Alkhovsky SV, Avšič-Županc T, Bente DA, Bergeron É, Burt F, Di Paola N, Ergünay K, Hewson R, Mirazimi A, Sall AA, Spengler JR, Postler TS, Palacios G, and Kuhn JH
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- Genotype, Humans, Hemorrhagic Fever Virus, Crimean-Congo genetics, Hemorrhagic Fever, Crimean
- Abstract
Crimean-Congo haemorrhagic fever virus (CCHFV) is the medically most important member of the rapidly expanding bunyaviral family Nairoviridae . Traditionally, CCHFV isolates have been assigned to six distinct genotypes. Here, the International Committee on Taxonomy of Viruses (ICTV) Nairoviridae Study Group outlines the reasons for the recent decision to re-classify genogroup VI (aka Europe-2 or AP-92-like) as a distinct virus, Aigai virus (AIGV).
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- 2022
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30. Parasite resistance and parasite tolerance: insights into transgenerational immune priming in an invertebrate host.
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Paraskevopoulou S, Gattis S, and Ben-Ami F
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- Animals, Daphnia, Parasites
- Abstract
Parasites impose different selection regimes on their hosts, which respond by increasing their resistance and/or tolerance. Parental challenge with parasites can enhance the immune response of their offspring, a phenomenon documented in invertebrates and termed transgenerational immune priming. We exposed two parental generations of the model organism Daphnia magna to the horizontally transmitted parasitic yeast Metschnikowia bicuspidata and recorded resistance- and tolerance-related traits in the offspring generation. We hypothesized that parentally primed offspring will increase either their resistance or their tolerance to the parasite. Our susceptibility assays revealed no impact of parental exposure on offspring resistance. Nonetheless, different fitness-related traits, which are indicative of tolerance, were altered. Specifically, maternal priming increased offspring production and decreased survival. Grandmaternal priming positively affected age at first reproduction and negatively affected brood size at first reproduction. Interestingly, both maternal and grandmaternal priming significantly reduced within-host-parasite proliferation. Nevertheless, Daphnia primed for two consecutive generations had no competitive advantage in comparison to unprimed ones, implying additive maternal and grandmaternal effects. Our findings do not support evidence of transgenerational immune priming from bacterial infections in the same host species, thus, emphasizing that transgenerational immune responses may not be consistent even within the same host species.
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- 2022
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31. Jingchuvirales : a New Taxonomical Framework for a Rapidly Expanding Order of Unusual Monjiviricete Viruses Broadly Distributed among Arthropod Subphyla.
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Di Paola N, Dheilly NM, Junglen S, Paraskevopoulou S, Postler TS, Shi M, and Kuhn JH
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- Animals, Genome, Viral, Metagenomics, Phylogeny, Arthropods, RNA Viruses genetics, Viruses genetics
- Abstract
Technical advances in metagenomics and metatranscriptomics have dramatically accelerated virus discovery in recent years. "Chuviruses" were first described in 2015 as obscure negative-sense RNA viruses of diverse arthropods. Although "chuviruses" first appeared to be members of the negarnaviricot order Mononegavirales in phylogenetic analyses using RNA-directed RNA polymerase sequences, further characterization revealed unusual gene orders in genomes that are nonsegmented, segmented, and/or possibly circular. Consequently, a separate order, Jingchuvirales , was established to include a monospecific family, Chuviridae . Recently, it has become apparent that jingchuvirals are broadly distributed and are therefore likely of ecological and economic importance. Here, we describe recent and ongoing efforts to create the necessary taxonomic framework to accommodate the expected flood of novel viruses belonging to the order.
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- 2022
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32. Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.
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Kuhn JH, Adkins S, Agwanda BR, Al Kubrusli R, Alkhovsky SV, Amarasinghe GK, Avšič-Županc T, Ayllón MA, Bahl J, Balkema-Buschmann A, Ballinger MJ, Basler CF, Bavari S, Beer M, Bejerman N, Bennett AJ, Bente DA, Bergeron É, Bird BH, Blair CD, Blasdell KR, Blystad DR, Bojko J, Borth WB, Bradfute S, Breyta R, Briese T, Brown PA, Brown JK, Buchholz UJ, Buchmeier MJ, Bukreyev A, Burt F, Büttner C, Calisher CH, Cao M, Casas I, Chandran K, Charrel RN, Cheng Q, Chiaki Y, Chiapello M, Choi IR, Ciuffo M, Clegg JCS, Crozier I, Dal Bó E, de la Torre JC, de Lamballerie X, de Swart RL, Debat H, Dheilly NM, Di Cicco E, Di Paola N, Di Serio F, Dietzgen RG, Digiaro M, Dolnik O, Drebot MA, Drexler JF, Dundon WG, Duprex WP, Dürrwald R, Dye JM, Easton AJ, Ebihara H, Elbeaino T, Ergünay K, Ferguson HW, Fooks AR, Forgia M, Formenty PBH, Fránová J, Freitas-Astúa J, Fu J, Fürl S, Gago-Zachert S, Gāo GF, García ML, García-Sastre A, Garrison AR, Gaskin T, Gonzalez JJ, Griffiths A, Goldberg TL, Groschup MH, Günther S, Hall RA, Hammond J, Han T, Hepojoki J, Hewson R, Hong J, Hong N, Hongo S, Horie M, Hu JS, Hu T, Hughes HR, Hüttner F, Hyndman TH, Ilyas M, Jalkanen R, Jiāng D, Jonson GB, Junglen S, Kadono F, Kaukinen KH, Kawate M, Klempa B, Klingström J, Kobinger G, Koloniuk I, Kondō H, Koonin EV, Krupovic M, Kubota K, Kurath G, Laenen L, Lambert AJ, Langevin SL, Lee B, Lefkowitz EJ, Leroy EM, Li S, Li L, Lǐ J, Liu H, Lukashevich IS, Maes P, de Souza WM, Marklewitz M, Marshall SH, Marzano SL, Massart S, McCauley JW, Melzer M, Mielke-Ehret N, Miller KM, Ming TJ, Mirazimi A, Mordecai GJ, Mühlbach HP, Mühlberger E, Naidu R, Natsuaki T, Navarro JA, Netesov SV, Neumann G, Nowotny N, Nunes MRT, Olmedo-Velarde A, Palacios G, Pallás V, Pályi B, Papa A, Paraskevopoulou S, Park AC, Parrish CR, Patterson DA, Pauvolid-Corrêa A, Pawęska JT, Payne S, Peracchio C, Pérez DR, Postler TS, Qi L, Radoshitzky SR, Resende RO, Reyes CA, Rima BK, Luna GR, Romanowski V, Rota P, Rubbenstroth D, Rubino L, Runstadler JA, Sabanadzovic S, Sall AA, Salvato MS, Sang R, Sasaya T, Schulze AD, Schwemmle M, Shi M, Shí X, Shí Z, Shimomoto Y, Shirako Y, Siddell SG, Simmonds P, Sironi M, Smagghe G, Smither S, Song JW, Spann K, Spengler JR, Stenglein MD, Stone DM, Sugano J, Suttle CA, Tabata A, Takada A, Takeuchi S, Tchouassi DP, Teffer A, Tesh RB, Thornburg NJ, Tomitaka Y, Tomonaga K, Tordo N, Torto B, Towner JS, Tsuda S, Tu C, Turina M, Tzanetakis IE, Uchida J, Usugi T, Vaira AM, Vallino M, van den Hoogen B, Varsani A, Vasilakis N, Verbeek M, von Bargen S, Wada J, Wahl V, Walker PJ, Wang LF, Wang G, Wang Y, Wang Y, Waqas M, Wèi T, Wen S, Whitfield AE, Williams JV, Wolf YI, Wu J, Xu L, Yanagisawa H, Yang C, Yang Z, Zerbini FM, Zhai L, Zhang YZ, Zhang S, Zhang J, Zhang Z, and Zhou X
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- 2021
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33. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.
- Author
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Kuhn JH, Adkins S, Agwanda BR, Al Kubrusli R, Alkhovsky SV, Amarasinghe GK, Avšič-Županc T, Ayllón MA, Bahl J, Balkema-Buschmann A, Ballinger MJ, Basler CF, Bavari S, Beer M, Bejerman N, Bennett AJ, Bente DA, Bergeron É, Bird BH, Blair CD, Blasdell KR, Blystad DR, Bojko J, Borth WB, Bradfute S, Breyta R, Briese T, Brown PA, Brown JK, Buchholz UJ, Buchmeier MJ, Bukreyev A, Burt F, Büttner C, Calisher CH, Cao M, Casas I, Chandran K, Charrel RN, Cheng Q, Chiaki Y, Chiapello M, Choi IR, Ciuffo M, Clegg JCS, Crozier I, Dal Bó E, de la Torre JC, de Lamballerie X, de Swart RL, Debat H, Dheilly NM, Di Cicco E, Di Paola N, Di Serio F, Dietzgen RG, Digiaro M, Dolnik O, Drebot MA, Drexler JF, Dundon WG, Duprex WP, Dürrwald R, Dye JM, Easton AJ, Ebihara H, Elbeaino T, Ergünay K, Ferguson HW, Fooks AR, Forgia M, Formenty PBH, Fránová J, Freitas-Astúa J, Fu J, Fürl S, Gago-Zachert S, Gāo GF, García ML, García-Sastre A, Garrison AR, Gaskin T, Gonzalez JJ, Griffiths A, Goldberg TL, Groschup MH, Günther S, Hall RA, Hammond J, Han T, Hepojoki J, Hewson R, Hong J, Hong N, Hongo S, Horie M, Hu JS, Hu T, Hughes HR, Hüttner F, Hyndman TH, Ilyas M, Jalkanen R, Jiāng D, Jonson GB, Junglen S, Kadono F, Kaukinen KH, Kawate M, Klempa B, Klingström J, Kobinger G, Koloniuk I, Kondō H, Koonin EV, Krupovic M, Kubota K, Kurath G, Laenen L, Lambert AJ, Langevin SL, Lee B, Lefkowitz EJ, Leroy EM, Li S, Li L, Lǐ J, Liu H, Lukashevich IS, Maes P, de Souza WM, Marklewitz M, Marshall SH, Marzano SL, Massart S, McCauley JW, Melzer M, Mielke-Ehret N, Miller KM, Ming TJ, Mirazimi A, Mordecai GJ, Mühlbach HP, Mühlberger E, Naidu R, Natsuaki T, Navarro JA, Netesov SV, Neumann G, Nowotny N, Nunes MRT, Olmedo-Velarde A, Palacios G, Pallás V, Pályi B, Papa A, Paraskevopoulou S, Park AC, Parrish CR, Patterson DA, Pauvolid-Corrêa A, Pawęska JT, Payne S, Peracchio C, Pérez DR, Postler TS, Qi L, Radoshitzky SR, Resende RO, Reyes CA, Rima BK, Luna GR, Romanowski V, Rota P, Rubbenstroth D, Rubino L, Runstadler JA, Sabanadzovic S, Sall AA, Salvato MS, Sang R, Sasaya T, Schulze AD, Schwemmle M, Shi M, Shí X, Shí Z, Shimomoto Y, Shirako Y, Siddell SG, Simmonds P, Sironi M, Smagghe G, Smither S, Song JW, Spann K, Spengler JR, Stenglein MD, Stone DM, Sugano J, Suttle CA, Tabata A, Takada A, Takeuchi S, Tchouassi DP, Teffer A, Tesh RB, Thornburg NJ, Tomitaka Y, Tomonaga K, Tordo N, Torto B, Towner JS, Tsuda S, Tu C, Turina M, Tzanetakis IE, Uchida J, Usugi T, Vaira AM, Vallino M, van den Hoogen B, Varsani A, Vasilakis N, Verbeek M, von Bargen S, Wada J, Wahl V, Walker PJ, Wang LF, Wang G, Wang Y, Wang Y, Waqas M, Wèi T, Wen S, Whitfield AE, Williams JV, Wolf YI, Wu J, Xu L, Yanagisawa H, Yang C, Yang Z, Zerbini FM, Zhai L, Zhang YZ, Zhang S, Zhang J, Zhang Z, and Zhou X
- Subjects
- Humans, Mononegavirales, Viruses
- Abstract
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV., (© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.)
- Published
- 2021
- Full Text
- View/download PDF
34. ICTV Virus Taxonomy Profile: Nyamiviridae 2021.
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Dietzgen RG, Firth AE, Jiāng D, Junglen S, Kondo H, Kuhn JH, Paraskevopoulou S, Vasilakis N, and Ictv Report Consortium
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- Animals, Genome, Viral, Invertebrates virology, Mononegavirales genetics, Phylogeny, RNA, Viral genetics, Viral Proteins genetics, Virion classification, Virion genetics, Virion isolation & purification, Mononegavirales classification, Mononegavirales isolation & purification
- Abstract
Nyamiviridae is a family of viruses in the order Mononegavirales , with unsegmented (except for members of the genus Tapwovirus ), negative-sense RNA genomes of 10-13 kb. Nyamviruses have a genome organisation and content similar to that of other mononegaviruses. Nyamiviridae includes several genera that form monophyletic clades on phylogenetic analysis of the RNA polymerase. Nyamiviruses have been found associated with diverse invertebrates as well as land- and seabirds. Members of the genera Nyavirus and Socyvirus produce enveloped, spherical virions. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Nyamiviridae, which is available at ictv.global/report/nyamiviridae.
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- 2021
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35. Favorable antibody responses to human coronaviruses in children and adolescents with autoimmune rheumatic diseases.
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Deakin CT, Cornish GH, Ng KW, Faulkner N, Bolland W, Hope J, Rosa A, Harvey R, Hussain S, Earl C, Jebson BR, Wilkinson MGLL, Marshall LR, O'Brien K, Rosser EC, Radziszewska A, Peckham H, Patel H, Heaney J, Rickman H, Paraskevopoulou S, Houlihan CF, Spyer MJ, Gamblin SJ, McCauley J, Nastouli E, Levin M, Cherepanov P, Ciurtin C, Wedderburn LR, and Kassiotis G
- Subjects
- Adolescent, Adult, Antibodies, Viral, Antibody Formation, Child, Humans, Immunoglobulin G, Nucleoproteins, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Systemic Inflammatory Response Syndrome, Autoimmune Diseases, COVID-19 complications, Coronavirus OC43, Human, Rheumatic Diseases
- Abstract
Background: Differences in humoral immunity to coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), between children and adults remain unexplained, and the effect of underlying immune dysfunction or suppression is unknown. Here, we sought to examine the antibody immune competence of children and adolescents with prevalent inflammatory rheumatic diseases, juvenile idiopathic arthritis (JIA), juvenile dermatomyositis (JDM), and juvenile systemic lupus erythematosus (JSLE) against the seasonal human coronavirus (HCoV)-OC43 that frequently infects this age group., Methods: Sera were collected from JIA (n = 118), JDM (n = 49), and JSLE (n = 30) patients and from healthy control (n = 54) children and adolescents prior to the coronavirus disease 19 (COVID-19) pandemic. We used sensitive flow-cytometry-based assays to determine titers of antibodies that reacted with the spike and nucleoprotein of HCoV-OC43 and cross-reacted with the spike and nucleoprotein of SARS-CoV-2, and we compared them with respective titers in sera from patients with multisystem inflammatory syndrome in children and adolescents (MIS-C)., Findings: Despite immune dysfunction and immunosuppressive treatment, JIA, JDM, and JSLE patients maintained comparable or stronger humoral responses than healthier peers, which was dominated by immunoglobulin G (IgG) antibodies to HCoV-OC43 spike, and harbored IgG antibodies that cross-reacted with SARS-CoV-2 spike. In contrast, responses to HCoV-OC43 and SARS-CoV-2 nucleoproteins exhibited delayed age-dependent class-switching and were not elevated in JIA, JDM, and JSLE patients, which argues against increased exposure., Conclusions: Consequently, autoimmune rheumatic diseases and their treatment were associated with a favorable ratio of spike to nucleoprotein antibodies., Funding: This work was supported by a Centre of Excellence Centre for Adolescent Rheumatology Versus Arthritis grant, 21593, UKRI funding reference MR/R013926/1, the Great Ormond Street Children's Charity, Cure JM Foundation, Myositis UK, Lupus UK, and the NIHR Biomedical Research Centres at GOSH and UCLH. This work was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK, the UK Medical Research Council, and the Wellcome Trust ., Competing Interests: The authors declare no competing interests., (© 2021 Elsevier Inc.)
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- 2021
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36. Reduced antibody cross-reactivity following infection with B.1.1.7 than with parental SARS-CoV-2 strains.
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Faulkner N, Ng KW, Wu MY, Harvey R, Margaritis M, Paraskevopoulou S, Houlihan C, Hussain S, Greco M, Bolland W, Warchal S, Heaney J, Rickman H, Spyer M, Frampton D, Byott M, de Oliveira T, Sigal A, Kjaer S, Swanton C, Gandhi S, Beale R, Gamblin SJ, McCauley JW, Daniels RS, Howell M, Bauer D, Nastouli E, and Kassiotis G
- Subjects
- Antibodies, Neutralizing immunology, COVID-19 epidemiology, Cross Reactions, Humans, Parents, South Africa epidemiology, Spike Glycoprotein, Coronavirus, United Kingdom epidemiology, Antibodies, Viral immunology, COVID-19 immunology, COVID-19 virology, SARS-CoV-2 immunology
- Abstract
Background: The degree of heterotypic immunity induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains is a major determinant of the spread of emerging variants and the success of vaccination campaigns, but remains incompletely understood., Methods: We examined the immunogenicity of SARS-CoV-2 variant B.1.1.7 (Alpha) that arose in the United Kingdom and spread globally. We determined titres of spike glycoprotein-binding antibodies and authentic virus neutralising antibodies induced by B.1.1.7 infection to infer homotypic and heterotypic immunity., Results: Antibodies elicited by B.1.1.7 infection exhibited significantly reduced recognition and neutralisation of parental strains or of the South Africa variant B.1.351 (Beta) than of the infecting variant. The drop in cross-reactivity was significantly more pronounced following B.1.1.7 than parental strain infection., Conclusions: The results indicate that heterotypic immunity induced by SARS-CoV-2 variants is asymmetric., Funding: This work was supported by the Francis Crick Institute and the Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg., Competing Interests: NF, KN, MW, RH, MM, SP, CH, SH, MG, WB, SW, JH, HR, MS, DF, MB, Td, AS, SK, CS, SG, RB, SG, JM, RD, MH, DB, EN, GK No competing interests declared, (© 2021, Faulkner et al.)
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- 2021
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37. Adaptive and nonadaptive plasticity in changing environments: Implications for sexual species with different life history strategies.
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Romero-Mujalli D, Rochow M, Kahl S, Paraskevopoulou S, Folkertsma R, Jeltsch F, and Tiedemann R
- Abstract
Populations adapt to novel environmental conditions by genetic changes or phenotypic plasticity. Plastic responses are generally faster and can buffer fitness losses under variable conditions. Plasticity is typically modeled as random noise and linear reaction norms that assume simple one-to-one genotype-phenotype maps and no limits to the phenotypic response. Most studies on plasticity have focused on its effect on population viability. However, it is not clear, whether the advantage of plasticity depends solely on environmental fluctuations or also on the genetic and demographic properties (life histories) of populations. Here we present an individual-based model and study the relative importance of adaptive and nonadaptive plasticity for populations of sexual species with different life histories experiencing directional stochastic climate change. Environmental fluctuations were simulated using differentially autocorrelated climatic stochasticity or noise color, and scenarios of directional climate change. Nonadaptive plasticity was simulated as a random environmental effect on trait development, while adaptive plasticity as a linear, saturating, or sinusoidal reaction norm. The last two imposed limits to the plastic response and emphasized flexible interactions of the genotype with the environment. Interestingly, this assumption led to (a) smaller phenotypic than genotypic variance in the population (many-to-one genotype-phenotype map) and the coexistence of polymorphisms, and (b) the maintenance of higher genetic variation-compared to linear reaction norms and genetic determinism-even when the population was exposed to a constant environment for several generations. Limits to plasticity led to genetic accommodation, when costs were negligible, and to the appearance of cryptic variation when limits were exceeded. We found that adaptive plasticity promoted population persistence under red environmental noise and was particularly important for life histories with low fecundity. Populations producing more offspring could cope with environmental fluctuations solely by genetic changes or random plasticity, unless environmental change was too fast., Competing Interests: None., (© 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)
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- 2021
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38. Viromics of extant insect orders unveil the evolution of the flavi-like superfamily.
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Paraskevopoulou S, Käfer S, Zirkel F, Donath A, Petersen M, Liu S, Zhou X, Drosten C, Misof B, and Junglen S
- Abstract
Insects are the most diversified and species-rich group of animals and harbor an immense diversity of viruses. Several taxa in the flavi-like superfamily, such as the genus Flavivirus , are associated with insects; however, systematic studies on insect virus genetic diversity are lacking, limiting our understanding of the evolution of the flavi-like superfamily. Here, we examined the diversity of flavi-like viruses within the most complete and up-to-date insect transcriptome collection comprising 1,243 insect species by employing a Flaviviridae RdRp profile hidden Markov model search. We identified seventy-six viral sequences in sixty-one species belonging to seventeen insect, one entognathan, and one arachnidan orders. Phylogenetic analyses revealed that twenty-seven sequences fell within the Flaviviridae phylogeny but did not group with established genera. Despite the large diversity of insect hosts studied, we only detected one virus in a blood-feeding insect, which branched within the genus Flavivirus , indicating that this genus likely diversified only in hematophagous arthropods. Nine new jingmenviruses with novel host associations were identified. One of the jingmenviruses established a deep rooting lineage additional to the insect- and tick-associated clades. Segment co-segregation phylogenies support the separation of tick- and insect-associated groups within jingmenviruses, with evidence for segment reassortment. In addition, fourteen viruses grouped with unclassified flaviviruses encompassing genome length of up to 20 kb. Species-specific clades for Hymenopteran- and Orthopteran-associated viruses were identified. Forty-nine viruses populated three highly diversified clades in distant relationship to Tombusviridae , a plant-infecting virus family, suggesting the detection of three previously unknown insect-associated families that contributed to tombusvirus evolution., (© The Author(s) 2021. Published by Oxford University Press.)
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- 2021
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39. Genomic consequences of human-mediated translocations in margin populations of an endangered amphibian.
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De Cahsan B, Westbury MV, Paraskevopoulou S, Drews H, Ott M, Gollmann G, and Tiedemann R
- Abstract
Due to their isolated and often fragmented nature, range margin populations are especially vulnerable to rapid environmental change. To maintain genetic diversity and adaptive potential, gene flow from disjunct populations might therefore be crucial to their survival. Translocations are often proposed as a mitigation strategy to increase genetic diversity in threatened populations. However, this also includes the risk of losing locally adapted alleles through genetic swamping. Human-mediated translocations of southern lineage specimens into northern German populations of the endangered European fire-bellied toad ( Bombina bombina ) provide an unexpected experimental set-up to test the genetic consequences of an intraspecific introgression from central population individuals into populations at the species range margin. Here, we utilize complete mitochondrial genomes and transcriptome nuclear data to reveal the full genetic extent of this translocation and the consequences it may have for these populations. We uncover signs of introgression in four out of the five northern populations investigated, including a number of introgressed alleles ubiquitous in all recipient populations, suggesting a possible adaptive advantage. Introgressed alleles dominate at the MTCH2 locus, associated with obesity/fat tissue in humans, and the DSP locus, essential for the proper development of epidermal skin in amphibians. Furthermore, we found loci where local alleles were retained in the introgressed populations, suggesting their relevance for local adaptation. Finally, comparisons of genetic diversity between introgressed and nonintrogressed northern German populations revealed an increase in genetic diversity in all German individuals belonging to introgressed populations, supporting the idea of a beneficial transfer of genetic variation from Austria into North Germany., Competing Interests: The authors declare that they have no conflict of interest., (© 2021 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd.)
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- 2021
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40. Preexisting and de novo humoral immunity to SARS-CoV-2 in humans.
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Ng KW, Faulkner N, Cornish GH, Rosa A, Harvey R, Hussain S, Ulferts R, Earl C, Wrobel AG, Benton DJ, Roustan C, Bolland W, Thompson R, Agua-Doce A, Hobson P, Heaney J, Rickman H, Paraskevopoulou S, Houlihan CF, Thomson K, Sanchez E, Shin GY, Spyer MJ, Joshi D, O'Reilly N, Walker PA, Kjaer S, Riddell A, Moore C, Jebson BR, Wilkinson M, Marshall LR, Rosser EC, Radziszewska A, Peckham H, Ciurtin C, Wedderburn LR, Beale R, Swanton C, Gandhi S, Stockinger B, McCauley J, Gamblin SJ, McCoy LE, Cherepanov P, Nastouli E, and Kassiotis G
- Subjects
- Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Animals, COVID-19 blood, Epitope Mapping, Female, HEK293 Cells, Humans, Immunoglobulin A blood, Immunoglobulin G blood, Immunoglobulin M blood, Male, Middle Aged, SARS-CoV-2 chemistry, Spike Glycoprotein, Coronavirus chemistry, Viral Zoonoses blood, Viral Zoonoses immunology, Young Adult, Antibodies, Viral blood, COVID-19 immunology, Immunity, Humoral, SARS-CoV-2 immunology, Spike Glycoprotein, Coronavirus immunology
- Abstract
Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
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- 2020
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41. The International Virus Bioinformatics Meeting 2020.
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Hufsky F, Beerenwinkel N, Meyer IM, Roux S, Cook GM, Kinsella CM, Lamkiewicz K, Marquet M, Nieuwenhuijse DF, Olendraite I, Paraskevopoulou S, Young F, Dijkman R, Ibrahim B, Kelly J, Le Mercier P, Marz M, Ramette A, and Thiel V
- Subjects
- COVID-19, Congresses as Topic, Evolution, Molecular, Genome, Viral, Humans, Metagenomics, RNA Viruses pathogenicity, Computational Biology, RNA Viruses genetics, Virology
- Abstract
The International Virus Bioinformatics Meeting 2020 was originally planned to take place in Bern, Switzerland, in March 2020. However, the COVID-19 pandemic put a spoke in the wheel of almost all conferences to be held in 2020. After moving the conference to 8-9 October 2020, we got hit by the second wave and finally decided at short notice to go fully online. On the other hand, the pandemic has made us even more aware of the importance of accelerating research in viral bioinformatics. Advances in bioinformatics have led to improved approaches to investigate viral infections and outbreaks. The International Virus Bioinformatics Meeting 2020 has attracted approximately 120 experts in virology and bioinformatics from all over the world to join the two-day virtual meeting. Despite concerns being raised that virtual meetings lack possibilities for face-to-face discussion, the participants from this small community created a highly interactive scientific environment, engaging in lively and inspiring discussions and suggesting new research directions and questions. The meeting featured five invited and twelve contributed talks, on the four main topics: (1) proteome and RNAome of RNA viruses, (2) viral metagenomics and ecology, (3) virus evolution and classification and (4) viral infections and immunology. Further, the meeting featured 20 oral poster presentations, all of which focused on specific areas of virus bioinformatics. This report summarizes the main research findings and highlights presented at the meeting.
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- 2020
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42. Temperature-dependent life history and transcriptomic responses in heat-tolerant versus heat-sensitive Brachionus rotifers.
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Paraskevopoulou S, Dennis AB, Weithoff G, and Tiedemann R
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- Adaptation, Physiological, Animals, Climate Change, Female, Male, Phenotype, Rotifera classification, Rotifera genetics, Stress, Physiological, Temperature, Gene Expression Profiling methods, Heat-Shock Proteins genetics, Rotifera physiology
- Abstract
Thermal stress response is an essential physiological trait that determines occurrence and temporal succession in nature, including response to climate change. We compared temperature-related demography in closely related heat-tolerant and heat-sensitive Brachionus rotifer species. We found significant differences in heat response, with the heat-sensitive species adopting a strategy of long survival and low population growth, while the heat-tolerant followed the opposite strategy. In both species, we examined the genetic basis of physiological variation by comparing gene expression across increasing temperatures. Comparative transcriptomic analyses identified shared and opposing responses to heat. Interestingly, expression of heat shock proteins (hsps) was strikingly different in the two species and mirrored differences in population growth rates, showing that hsp genes are likely a key component of a species' adaptation to different temperatures. Temperature induction caused opposing patterns of expression in further functional categories including energy, carbohydrate and lipid metabolism, and in genes related to ribosomal proteins. In the heat-sensitive species, elevated temperatures caused up-regulation of genes related to meiosis induction and post-translational histone modifications. This work demonstrates the sweeping reorganizations of biological functions that accompany temperature adaptation in these two species and reveals potential molecular mechanisms that might be activated for adaptation to global warming.
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- 2020
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43. Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus .
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Paraskevopoulou S, Pirzer F, Goldmann N, Schmid J, Corman VM, Gottula LT, Schroeder S, Rasche A, Muth D, Drexler JF, Heni AC, Eibner GJ, Page RA, Jones TC, Müller MA, Sommer S, Glebe D, and Drosten C
- Subjects
- Animals, Cell Line, Tumor, Genome, Viral, Genomics methods, Hepadnaviridae classification, Hepatitis Delta Virus classification, Humans, Phylogeny, Coinfection, Hepadnaviridae physiology, Hepadnaviridae Infections veterinary, Hepatitis D veterinary, Hepatitis Delta Virus physiology, Rodent Diseases virology, Rodentia virology
- Abstract
Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a mammalian deltavirus that does not occur in humans, identified in the neotropical rodent species Proechimys semispinosus The rodent deltavirus is highly distinct, showing a common ancestor with a recently described deltavirus in snakes. Reverse genetics based on a tandem minus-strand complementary DNA genome copy under the control of a cytomegalovirus (CMV) promoter confirms autonomous genome replication in transfected cells, with initiation of replication from the upstream genome copy. In contrast to HDV, a large delta antigen is not expressed and the farnesylation motif critical for HBV interaction is absent from a genome region that might correspond to a hypothetical rodent large delta antigen. Correspondingly, there is no evidence for coinfection with an HBV-related hepadnavirus based on virus detection and serology in any deltavirus-positive animal. No other coinfecting viruses were detected by RNA sequencing studies of 120 wild-caught animals that could serve as a potential helper virus. The presence of virus in blood and pronounced detection in reproductively active males suggest horizontal transmission linked to competitive behavior. Our study establishes a nonhuman, mammalian deltavirus that occurs as a horizontally transmitted infection, is potentially cleared by immune response, is not focused in the liver, and possibly does not require helper virus coinfection., Competing Interests: The authors declare no competing interest., (Copyright © 2020 the Author(s). Published by PNAS.)
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- 2020
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44. Re-assessing the diversity of negative strand RNA viruses in insects.
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Käfer S, Paraskevopoulou S, Zirkel F, Wieseke N, Donath A, Petersen M, Jones TC, Liu S, Zhou X, Middendorf M, Junglen S, Misof B, and Drosten C
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- Animals, Insecta virology, RNA Virus Infections virology, RNA Viruses
- Abstract
The spectrum of viruses in insects is important for subjects as diverse as public health, veterinary medicine, food production, and biodiversity conservation. The traditional interest in vector-borne diseases of humans and livestock has drawn the attention of virus studies to hematophagous insect species. However, these represent only a tiny fraction of the broad diversity of Hexapoda, the most speciose group of animals. Here, we systematically probed the diversity of negative strand RNA viruses in the largest and most representative collection of insect transcriptomes from samples representing all 34 extant orders of Hexapoda and 3 orders of Entognatha, as well as outgroups, altogether representing 1243 species. Based on profile hidden Markov models we detected 488 viral RNA-directed RNA polymerase (RdRp) sequences with similarity to negative strand RNA viruses. These were identified in members of 324 arthropod species. Selection for length, quality, and uniqueness left 234 sequences for analyses, showing similarity to genomes of viruses classified in Bunyavirales (n = 86), Articulavirales (n = 54), and several orders within Haploviricotina (n = 94). Coding-complete genomes or nearly-complete subgenomic assemblies were obtained in 61 cases. Based on phylogenetic topology and the availability of coding-complete genomes we estimate that at least 20 novel viral genera in seven families need to be defined, only two of them monospecific. Seven additional viral clades emerge when adding sequences from the present study to formerly monospecific lineages, potentially requiring up to seven additional genera. One long sequence may indicate a novel family. For segmented viruses, cophylogenies between genome segments were generally improved by the inclusion of viruses from the present study, suggesting that in silico misassembly of segmented genomes is rare or absent. Contrary to previous assessments, significant virus-host codivergence was identified in major phylogenetic lineages based on two different approaches of codivergence analysis in a hypotheses testing framework. In spite of these additions to the known spectrum of viruses in insects, we caution that basing taxonomic decisions on genome information alone is challenging due to technical uncertainties, such as the inability to prove integrity of complete genome assemblies of segmented viruses., Competing Interests: The authors have declared that no competing interests exist.
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- 2019
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45. Within species expressed genetic variability and gene expression response to different temperatures in the rotifer Brachionus calyciflorus sensu stricto.
- Author
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Paraskevopoulou S, Dennis AB, Weithoff G, Hartmann S, and Tiedemann R
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- Animals, DNA, Mitochondrial genetics, Ecosystem, Gene Flow, Genetic Drift, Molecular Sequence Annotation, Rotifera classification, Selection, Genetic, Species Specificity, Temperature, Genes, Helminth, Genetic Speciation, Genetic Variation, Metabolic Networks and Pathways genetics, Phylogeny, Rotifera genetics
- Abstract
Genetic divergence is impacted by many factors, including phylogenetic history, gene flow, genetic drift, and divergent selection. Rotifers are an important component of aquatic ecosystems, and genetic variation is essential to their ongoing adaptive diversification and local adaptation. In addition to coding sequence divergence, variation in gene expression may relate to variable heat tolerance, and can impose ecological barriers within species. Temperature plays a significant role in aquatic ecosystems by affecting species abundance, spatio-temporal distribution, and habitat colonization. Recently described (formerly cryptic) species of the Brachionus calyciflorus complex exhibit different temperature tolerance both in natural and in laboratory studies, and show that B. calyciflorus sensu stricto (s.s.) is a thermotolerant species. Even within B. calyciflorus s.s., there is a tendency for further temperature specializations. Comparison of expressed genes allows us to assess the impact of stressors on both expression and sequence divergence among disparate populations within a single species. Here, we have used RNA-seq to explore expressed genetic diversity in B. calyciflorus s.s. in two mitochondrial DNA lineages with different phylogenetic histories and differences in thermotolerance. We identify a suite of candidate genes that may underlie local adaptation, with a particular focus on the response to sustained high or low temperatures. We do not find adaptive divergence in established candidate genes for thermal adaptation. Rather, we detect divergent selection among our two lineages in genes related to metabolism (lipid metabolism, metabolism of xenobiotics)., Competing Interests: The authors have declared that no competing interests exist.
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- 2019
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46. Diverse novel phleboviruses in sandflies from the Panama Canal area, Central Panama.
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Marklewitz M, Dutari LC, Paraskevopoulou S, Page RA, Loaiza JR, and Junglen S
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- Africa, Animals, Europe, Genome, Viral genetics, Humans, Insect Vectors virology, Panama, Phlebotomus Fever virology, Phylogeny, Phlebovirus genetics, Phlebovirus isolation & purification, Psychodidae virology
- Abstract
The genus Phlebovirus (order Bunyavirales, family Phenuiviridae) comprises 57 viruses that are grouped into nine species-complexes. Sandfly-transmitted phleboviruses are found in Europe, Africa and the Americas and are responsible for febrile illness and infections of the nervous system in humans. The aim of this study was to assess the genetic diversity of sandfly-transmitted phleboviruses in connected and isolated forest habitats throughout the Panama Canal area in Central Panama. In total, we collected 13 807 sandflies comprising eight phlebotomine species. We detected several strains pertaining to five previously unknown viruses showing maximum pairwise identities of 45-78 % to the RNA-dependent RNA polymerase genes of phleboviruses. Entire coding regions were directly sequenced from infected sandflies as virus isolation in cell culture was not successful. The viruses were tentatively named La Gloria virus (LAGV), Mona Grita virus (MOGV), Peña Blanca virus (PEBV), Tico virus (TICV) and Tres Almendras virus (TRAV). Inferred phylogenies and p-distance-based analyses revealed that PEBV groups with the Bujaru phlebovirus species-complex, TRAV with the Candiru phlebovirus species-complex and MOGV belongs to the proposed Icoarci phlebovirus species-complex, whereas LAGV and TICV seem to be distant members of the Bujaru phlebovirus species-complex. No specific vector or habitat association was found for any of the five viruses. Relative abundance of sandflies was similar over habitat types. Our study shows that blood-feeding insects originating from remote and biodiverse habitats harbour multiple previously unknown phleboviruses. These viruses should be included in future surveillance studies to assess their geographic distribution and to elucidate if these viruses cause symptoms of disease in animals or humans.
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- 2019
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47. Differential response to heat stress among evolutionary lineages of an aquatic invertebrate species complex.
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Paraskevopoulou S, Tiedemann R, and Weithoff G
- Subjects
- Animals, Phylogeny, Species Specificity, Stress, Physiological, Biological Evolution, Hot Temperature adverse effects, Rotifera physiology
- Abstract
Under global warming scenarios, rising temperatures can constitute heat stress to which species may respond differentially. Within a described species, knowledge on cryptic diversity is of further relevance, as different lineages/cryptic species may respond differentially to environmental change. The Brachionus calyciflorus species complex (Rotifera), which was recently described using integrative taxonomy, is an essential component of aquatic ecosystems. Here, we tested the hypothesis that these (formerly cryptic) species differ in their heat tolerance. We assigned 47 clones with nuclear ITS1 (nuITS1) and mitochondrial COI (mtCOI) markers to evolutionary lineages, now named B. calyciflorus sensu stricto (s.s.) and B. fernandoi We selected 15 representative clones and assessed their heat tolerance as a bi-dimensional phenotypic trait affected by both the intensity and duration of heat stress. We found two distinct groups, with B. calyciflorus s.s. clones having higher heat tolerance than the novel species B. fernandoi This apparent temperature specialization among former cryptic species underscores the necessity of a sound species delimitation and assignment, when organismal responses to environmental changes are investigated., (© 2018 The Author(s).)
- Published
- 2018
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