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Preexisting and de novo humoral immunity to SARS-CoV-2 in humans.
- Source :
-
Science (New York, N.Y.) [Science] 2020 Dec 11; Vol. 370 (6522), pp. 1339-1343. Date of Electronic Publication: 2020 Nov 06. - Publication Year :
- 2020
-
Abstract
- Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.<br /> (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Amino Acid Sequence
Animals
COVID-19 blood
Epitope Mapping
Female
HEK293 Cells
Humans
Immunoglobulin A blood
Immunoglobulin G blood
Immunoglobulin M blood
Male
Middle Aged
SARS-CoV-2 chemistry
Spike Glycoprotein, Coronavirus chemistry
Viral Zoonoses blood
Viral Zoonoses immunology
Young Adult
Antibodies, Viral blood
COVID-19 immunology
Immunity, Humoral
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 370
- Issue :
- 6522
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 33159009
- Full Text :
- https://doi.org/10.1126/science.abe1107