34 results on '"Paolo, Dambruoso"'
Search Results
2. Percutaneous Coronary Sinus Catheterization With the ProPlege Catheter Under Transesophageal Echocardiography and Pressure Guidance
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Labriola, Cataldo, Greco, Francesco, Braccio, Maurizio, Paolo Dambruoso, Pier, Labriola, Giuseppe, and Paparella, Domenico
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- 2015
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3. Efficiency in CO2-utilization strategies: The case of styrene carbonate synthesis in microdroplets conditions
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Daniele Urbani, Caterina Rovegno, Alessandro Massi, Mumin Enis Leblebici, Emine Kayahan, Eleonora Polo, Paolo Dambruoso, dambruoso, paolo/0000-0003-3937-9671, Urbani, Daniele, Rovegno, Caterina, Massi, Alessandro, LEBLEBICI, Mumin enis, KAYAHAN, Emine, Polo, Eleonora, and Dambruoso, Paolo
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Technology ,Engineering, Chemical ,Science & Technology ,TRANSFORMATIONS ,Process Chemistry and Technology ,Chemistry, Multidisciplinary ,Cyclic carbonates ,Aerosol reactions ,Nebulization ,CAPTURE ,Chemistry ,Engineering ,Physical Sciences ,Chemical Engineering (miscellaneous) ,Microdroplets ,EPOXIDES ,Waste Management and Disposal ,CO2 utilization ,DIOXIDE - Abstract
The styrene oxide to styrene carbonate conversion performed in CO2 atmosphere, herein selected as a case study, was implemented in microdroplets (aerosol) reactions at the preparative scale (3.5 mmol of the starting material) and mild conditions (1 atm CO2 pressure), within a custom-made ultrasonic nebulization reactor. Upon opti-mization of the promoter stoichiometry (1 eq of 4.3 TEG/KI ratio) and methanol (MeOH) dilution (7.5 mL of 2.5 v/v MeOH/TEG), performances under mass transfer-limited conditions of this novel methodological paradigm have been compared at 25 degrees C and 50 degrees C with those implemented as: a) no-stirred, b) stirred, and c) sonicated bulk reactions. Complete selectivity and an apparent acceleration factor (AAF) of 1.9 was registered at both temperature for microdroplets reactions in respect with the sonicated counterparts, these latter performing better than the other bulk reactions. These significative efficiency improvements, candidate aerosol reactions as a preferred process intensification approach in the realm of effective CO2-utilization strategies and, in general, in the development and exploitation of gas-liquid two-phase reactions. The present work was supported by the NATO Science for Peace and Security Programme (NATO SPS, Grant No. MYP G5885, project acronym: TANGO). The authors are grateful to the funding agency.
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- 2023
4. One pot synthesis of new cross-linked chitosan-Schiff' base: Characterization, and anti-proliferative activities
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Mohammed Elhag, Huda E. Abdelwahab, Paolo Dambruoso, Galila A. Yacout, Adel Z. Nasr, Mohamed M. El Sadek, and Mohamed A. Mostafa
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Cell Survival ,Carboxylic Acids ,Antineoplastic Agents ,02 engineering and technology ,Biochemistry ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,X-Ray Diffraction ,Structural Biology ,Chlorocebus aethiops ,Spectroscopy, Fourier Transform Infrared ,Magic angle spinning ,Animals ,Humans ,MTT assay ,Fourier transform infrared spectroscopy ,Vero Cells ,Molecular Biology ,Schiff Bases ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,technology, industry, and agriculture ,Hydrogels ,Hep G2 Cells ,General Medicine ,Nuclear magnetic resonance spectroscopy ,Carbon-13 NMR ,021001 nanoscience & nanotechnology ,chemistry ,Self-healing hydrogels ,Vero cell ,0210 nano-technology ,Nuclear chemistry - Abstract
Four novel chitosan hydrogels were successfully synthesized through the cross-linking reaction of chitosan with different concentrations of ethyl 5-(3,5-dihydroxy-1,4-dioxan-2-yl)-2-methylfuran-3-carboxylate. Their structures were confirmed by Fourier transform infrared spectroscopy (FT-IR), 13C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy (CP/MAS 13C NMR), ultraviolet-visible spectroscopy, thermogravimetric analysis (TGA, DTA), and X-ray diffraction (XRD). Cytotoxicity on hepatocellular carcinoma (HepG-2) cell line and a normal African green monkey kidney (Vero) cell line were studied using the MTT assay. The resultant hydrogels showed a good inhibitory effect comparing to the un-modified parent; the hydrogels with the lowest degree cross-linking (0.125 and 0.25 mol cross-linker per one chitosan residue) showed potent anticancer activity in the HepG2 cells with IC50 of 57.9 and 80.9 μg/ml, respectively. These results show that the newly synthesized cross-linked chitosan derivatives demonstrated more selectivity to the HepG2 than the Vero cells, indicating its potential for Investigation in the cure of hepatocellular carcinoma.
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- 2021
5. Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate
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Jean-Maurice Mallet, Roberta Lanzillo, Francesca Nuti, Feliciana Real Fernández, Anna Maria Papini, Vincenzo Brescia Morra, Paolo Dambruoso, Antonio Mazzoleni, Paolo Rovero, Monica Bertoldo, Mazzoleni, Antonio, Real-Fernandez, Feliciana, Nuti, Francesca, Lanzillo, Roberta, Brescia Morra, Vincenzo, Dambruoso, Paolo, Bertoldo, Monica, Rovero, Paolo, Mallet, Jean-Maurice, Papini, Anna Maria, Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Università degli studi di Napoli Federico II, Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Viale dell'Università, Istituto per la Sintesi Organica e la Fotoreattività - ISOF (Bologne, Italie), Consiglio Nazionale delle Ricerche [Bologna] (CNR), Università degli Studi di Ferrara (UniFE), Laboratoire des biomolécules (LBM UMR 7203), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Paris sciences et lettres (PSL)
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Glycosylation ,dextran conjugate ,Peptide ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Epitope ,Sepharose ,PE5_7 ,chemistry.chemical_classification ,biology ,Molecular Structure ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,Microbial Sensitivity Test ,Bacterial ,Dextrans ,021001 nanoscience & nanotechnology ,Adhesins ,Autoantibodie ,peptide ,Anti-Bacterial Agents ,Molecular Medicine ,ELISA ,Antibody ,0210 nano-technology ,Human ,multivalence ,Microbial Sensitivity Tests ,010402 general chemistry ,Adhesins, Bacterial ,Autoantibodies ,Haemophilus influenzae ,Humans ,Peptides ,NO ,Antigen ,Anti-Bacterial Agent ,Avidity ,antibody capture ,Molecular Biology ,Dextran ,Organic Chemistry ,Molecular biology ,0104 chemical sciences ,Bacterial adhesin ,chemistry ,biology.protein ,antibody caption ,Conjugate - Abstract
International audience; Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools to increase the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity. We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1 of non-typeable Haemophilus influenzae (NTHi) . We selected the C-terminal HMW1(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N 3 )-NH 2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalysed alkyne-azide coupling reaction (CuAAC). Quantitative NMR measurements allowed to characterize the peptide loading (19.5%) on the multivalent dextran conjugate. This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera. Specific antibodies from a representative MS serum, were successfully depleted using a sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA. These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of patients affected by autoimmune patients, in general, and of specific high affinity antibodies against a minimal glycosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.
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- 2022
6. Gold nanoparticles supported on functionalized silica as catalysts for alkyne hydroamination: A chemico-physical insight
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Ilaria Ragazzini, Chiara Parise, Barbara Ballarin, Paolo Dambruoso, Daniele Nanni, Elisa Boanini, Davide Barreca, Maria Cristina Cassani, Parise C., Ballarin B., Barreca D., Cassani M.C., Dambruoso P., Nanni D., Ragazzini I., and Boanini E.
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Gold nanoparticle ,Materials science ,General Physics and Astronomy ,02 engineering and technology ,010402 general chemistry ,Heterogeneous catalysis ,01 natural sciences ,Hydroamination ,Catalysis ,Heterogeneous catalysi ,chemistry.chemical_compound ,Gold nanoparticles ,Surfaces and Interfaces ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Silanol ,Solid state NMR spectroscopy ,Phenylacetylene ,chemistry ,Chemical engineering ,Colloidal gold ,Propynylcarbamate-functionalized silica ,Surface modification ,0210 nano-technology ,Selectivity - Abstract
Highly stable gold nanoparticles anchored on propynylcarbamate-functionalized silica (Au/SiO2@Yne) have been efficiently utilized for the heterogeneous hydroamination of phenylacetylene with aniline under different reaction conditions. In order to ascertain the eventual influence of surface silanol groups on the system activity and selectivity tailored modifications of Au/SiO2@Yne catalysts were pursued according to two different strategies, involving respectively functionalization with trimethylethoxysilane (Au/SiO2@Yne-TMS) or post-treatment with triethylamine (Au/SiO2@Yne-NEt3). The prepared materials were analysed by several complementary techniques such as Solid State NMR (SS NMR), Transmission Electron Microscopy (TEM), X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD). A comparison of the resulting catalytic activities with that of the pristine Au/SiO2@Yne revealed a significant improvement for Au/SiO2@Yne-NEt3 in terms of both conversion and selectivity. Recycling and stability studies showed a catalytic activity decrease after the first run, due to the formation of polyphenylacetylene (PPhA) oligomers shielding the active sites. PPhA removal by sonication in acetone fully restored the catalytic activity and empowered the system with a good operational stability, a very crucial issue in view of eventual practical applications.
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- 2019
7. The Italian National Research Council Operations Within the EIT Raw Materials Framework
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Salvatore Siano, Vladimiro Dal Santo, Paolo Dambruoso, Armida Torreggiani, Stefania Marzocchi, and Ornella Russo
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Engineering management ,Lifelong learning ,Research council ,Political science ,Digital competences ,South east ,Raw materials ,Scientific literature ,Education - Abstract
The adopted strategy and the results achieved by the Italian National Research Council within the Knowledge and Innovation Community “Raw Materials” of the European Institute of Innovation and Technology (KIC EIT-RM) are presented in detail. We focus on activities dedicated to education as well as validation and acceleration actions of the EIT-RM. Regarding the former, activities tackling the awareness of citizens on the impact of RMs in our life, guiding pupils towards an informed engagement into RMs university carriers, and lifelong learning of professionals dedicated to methodologies to access, organize, and share scientific literature and data are presented. Regarding the validation and acceleration actions, two main activities are discussed: (1) development of Platinum–Group Metals free catalysts and the corresponding know-how transfer initiative towards East and South East Europe (ESEE) countries; (2) development of novel analytical logging tools and portable devices for real-time compositional analyses based on laser technologies.
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- 2021
8. Overcoming mass and photon transfer limitations in a scalable reactor: Oxidation in an aerosol photoreactor
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Daniele Urbani, M. Enis Leblebici, Leen Braeken, Emine Kayahan, Paolo Dambruoso, Alessandro Massi, and Tom Van Gerven
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Work (thermodynamics) ,Materials science ,General Chemical Engineering ,Nuclear engineering ,Flow (psychology) ,02 engineering and technology ,010402 general chemistry ,Aerosol reactor ,01 natural sciences ,Industrial and Manufacturing Engineering ,Reaction rate constant ,Mass transfer ,Environmental Chemistry ,Flow photochemistry ,Photoreactor ,Scale up ,Singlet oxygen ,Mass transfer coefficient ,Ambientale ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Aerosol ,Microreactor ,0210 nano-technology ,Order of magnitude - Abstract
The use of microflow reactors enabled dramatic improvements in the apparent rates of photochemical reactions. However, scaling-up of photomicroreactors remains a challenge due to the difficulties of distributing light and flow to all units of photomicroreactors. In this work, the mass and photon transfer limitations of an easily scalable micro-structured reactor, an aerosol photoreactor, was studied. In this reactor concept, each droplet works as a microreactor. The nature of aerosol light interaction enables good light distribution to all the droplets. A singlet oxygen mediated photosulfoxidation reaction was utilized as a model reaction to assess the reactor performance. The light transfer limitations were proven to be overcome in the aerosol photoreactor. The reaction rate constant was calculated as 0.12 s−1. A simple solution of the convection–diffusion equation for a droplet was presented to check for the mass transfer limitations in aerosol reactors. The Sauter mean diameters of the droplets at different pressures varied between 7 and 8 µm. The aerosol photoreactor was proven to overcome the mass transfer limitations at these droplet diameters. In addition, the highest volumetric mass transfer coefficient (kLa) was calculated as 1.2 s−1, which was at the same order of magnitude compared to other intensified photoreactors such as Corning Advanced Flow ReactorsTM or a gas–liquid microreactor. A discussion on increasing the throughput of aerosol photoreactors further is presented. This work paves the way for efficient and scalable photoreactors for industrial use.
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- 2021
9. Expanding the Toolbox of Heterogeneous Asymmetric Organocatalysts: Bifunctional Cyclopropenimine Superbases for Enantioselective Catalysis in Batch and Continuous Flow
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Olga Bortolini, Costanza Leonardi, Paolo Dambruoso, Arianna Brandolese, Eleonora Polo, Graziano Di Carmine, Alessandro Massi, Daniele Ragno, and Lorenzo Preti
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Chemistry ,flow chemistry ,Enantioselective synthesis ,Ambientale ,asymmetric catalysis ,packed-bed reactor ,General Chemistry ,Flow chemistry ,Heterogeneous catalysis ,Brønsted base ,Combinatorial chemistry ,asymmetric catalysis, Brønsted base, C-C coupling, flow chemistry, heterogeneous catalysis, immobilization, Michael addition, organocatalysis, packed-bed reactor ,Catalysis ,chemistry.chemical_compound ,Bronsted base ,heterogeneous catalysis ,Organocatalysis ,Michael addition ,immobilization ,Michael reaction ,organocatalysis ,C-C coupling ,Bifunctional ,Brønsted–Lowry acid–base theory - Abstract
A strategy for the immobilization of chiral 2,3-bisaminocyclopropenium salt (pre-catalyst) onto polystyrene and silica supports is presented together with a suitable procedure for the conversion into the corresponding cyclopropenimine superbase catalysts. The activity and recyclability of polystyrene- and silica-supported cyclopropenimines were initially tested under batch conditions in a model Michael addition detecting comparable efficiencies but a superior stability of the latter heterogeneous catalyst (5 cycles, accumulated TON of 27.1). The preferred silica-supported cyclopropenimine behaved very similarly to the soluble counterpart in the reaction of glycine imine with different Michael acceptors (48-92% yield; 60-98% ee) and it could be utilized as packing material for the fabrication of fixed-bed mesoreactors (pressure-resistant stainless-steel columns). Continuous-flow experiments were performed with satisfactory long-term stability (24 h on stream) with unaltered conversion efficiency and enantioselectivity.
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- 2021
10. Elucidating heteroatom influence on homonuclear 4J(H,H) coupling constants by DFT/NMR approach
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Paolo Dambruoso, Raffaele Riccio, Giuseppe Bifulco, Daniele Urbani, and Maria Giovanna Chini
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Heteroatom ,Dihedral angle ,010402 general chemistry ,01 natural sciences ,Homonuclear molecule ,Atom ,Molecule ,General Materials Science ,density function theory ,Alkyl ,H ,conformational analysis ,heteroatoms ,long-range coupling constant ,nuclear magnetic resonance ,organic synthesis ,chemistry.chemical_classification ,Coupling constant ,010405 organic chemistry ,General Chemistry ,0104 chemical sciences ,chemistry ,Physical chemistry ,Density functional theory - Abstract
We report the structural dependency of long range scalar J-coupling constant across four bonds as function of the dihedral angles Φ1 and Φ3. The calculated homonuclear coupling constants 4 J(H,H ), obtained at a density functional theory level, were measured between C(1)─X(2) and X(2)─C(3) bonds in three-term models, where C, N, O, and S were systematically used as the second atom of the alkyl structures (1-4). The 4 J(H,H) calculated values, tabulated for variation of 30° for both Φ1 and Φ3, have disclosed an unexpected detectable coupling constant (4 J(H,H ) ≥ 1 Hz) across heteroatoms, useful to provide valuable structural information. A 2-methyl-1,3-dithiane sulfide (5) was used as a case study to prove the applicability and reliability of the calculated values to real issues. The 4 J(H,H ) values obtained at density functional theory for the system 4 have reproduced with good accuracy an unexpected experimental 4 J(H2ax-H4ax ) = 1.01 Hz of sulfide molecule (5), suggesting these calculated coupling constant values as a new powerful tool for the organic synthesis and stereochemical analysis.
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- 2020
11. A community-built calibration system: The case study of quantification of metabolites in grape juice by qNMR spectroscopy
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Ales Čamra, John Warren, Taylor David, Dinesh Chalasani, Daniele Ragno, Jan Teipel, Tommaso Di Noia, Aurimas Bieliauskas, Elina Zailer-Hafer, Stefano Todisco, Domenico Acquotti, Lorraine M. Bateman, James Donarski, Piero Mastrorilli, Rosa Ragone, Cristina Airoldi, John S. Harwood, Michael Assfalg, Dolores Molero Vilchez, Francesco Longobardi, Magali Martin-Biran, Elisabetta Schievano, Domenico Mallamace, Elisabetta Torregiani, Biagia Musio, Stefania Pontrelli, Paolo Dambruoso, Marina Veronesi, Livio Stevanato, Augusta Caligiani, Erwann Hamon, Maurizio Triggiani, Davide Bertelli, Flaminia Cesare Marincola, Stefano Mammi, Bernd Diehl, Vito Gallo, Daniela Valensin, Bhavaraju Sitaram, Alessandro Barge, Claudia Di Napoli, Elena Sáez Barajas, Pasquale Scapicchio, Emanuela Callone, Antonino Rizzuti, Panteleimon G. Takis, Fabio Bertocchi, Anna Borioni, Cristiano Zuccaccia, Maria Cecilia Rossi, Mario Latronico, Andrea Kobrlová, Luca Goldoni, Nicola Intini, Freddy Thomas, Roberto Gobetto, Renzo Luisi, Ana M. Gil, Pierluigi Mazzei, Julien Wist, Roberto Consonni, Francesca Benevelli, Algirdas Šačkus, Antonio Randazzo, Salvatore Milone, Archimede Rotondo, Roger J. Mulder, Silvia Davalli, Andrea Mele, Musio, B, Ragone, R, Todisco, S, Rizzuti, A, Latronico, M, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Di Noia, T, Acquotti, D, Airoldi, C, Assfalg, M, Barge, A, Bateman, L, Benevelli, F, Bertelli, D, Bertocchi, F, Bieliauskas, A, Borioni, A, Caligiani, A, Callone, E, Čamra, A, Cesare Marincola, F, Chalasani, D, Consonni, R, Dambruoso, P, Davalli, S, David, T, Diehl, B, Donarski, J, Gil, A, Gobetto, R, Goldoni, L, Hamon, E, Harwood, J, Kobrlová, A, Longobardi, F, Luisi, R, Mallamace, D, Mammi, S, Martin-Biran, M, Mazzei, P, Mele, A, Milone, S, Molero Vilchez, D, Mulder, R, Napoli, C, Ragno, D, Randazzo, A, Rossi, M, Rotondo, A, Šačkus, A, Sáez Barajas, E, Schievano, E, Sitaram, B, Stevanato, L, Takis, P, Teipel, J, Thomas, F, Torregiani, E, Valensin, D, Veronesi, M, Warren, J, Wist, J, Zailer-Hafer, E, Zuccaccia, C, Gallo, V, Musio, B., Ragone, R., Todisco, S., Rizzuti, A., Latronico, M., Mastrorilli, P., Pontrelli, S., Intini, N., Scapicchio, P., Triggiani, M., Di Noia, T., Acquotti, D., Airoldi, C., Assfalg, M., Barge, A., Bateman, L., Benevelli, F., Bertelli, D., Bertocchi, F., Bieliauskas, A., Borioni, A., Caligiani, A., Callone, E., Camra, A., Cesare Marincola, F., Chalasani, D., Consonni, R., Dambruoso, P., Davalli, S., David, T., Diehl, B., Donarski, J., Gil, A. M., Gobetto, R., Goldoni, L., Hamon, E., Harwood, J. S., Kobrlova, A., Longobardi, F., Luisi, R., Mallamace, D., Mammi, S., Martin-Biran, M., Mazzei, P., Mele, A., Milone, S., Molero Vilchez, D., Mulder, R. J., Napoli, C., Ragno, D., Randazzo, A., Rossi, M. C., Rotondo, A., Sackus, A., Saez Barajas, E., Schievano, E., Sitaram, B., Stevanato, L., Takis, P. G., Teipel, J., Thomas, F., Torregiani, E., Valensin, D., Veronesi, M., Warren, J., Wist, J., Zailer-Hafer, E., Zuccaccia, C., and Gallo, V.
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Analyte ,Magnetic Resonance Spectroscopy ,Traceability ,qNMR Interlaboratory comparison Calibration Multiple regression Validation Food quality control ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,NO ,Food quality control ,Matrix (chemical analysis) ,Interlaboratory comparison ,Validation ,CHIM/06 - CHIMICA ORGANICA ,Calibration ,Calibration, Food quality control, Interlaboratory comparison, Multiple regression, qNMR, Validation ,Vitis ,qNMR ,Multiple regression ,qNMR, Interlaboratory comparison, Calibration, Multiple regression, Validation, Food quality control ,Spectroscopy ,Fruit and Vegetable Juices ,Reproducibility ,Spectrometer ,Chemistry ,010401 analytical chemistry ,Analytical technique ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,qNMR, interlaboratory comparison ,Fruit and Vegetable Juice ,0210 nano-technology ,Biological system - Abstract
Nuclear Magnetic Resonance (NMR) is an analytical technique extensively used in almost every chemical laboratory for structural identification. This technique provides statistically equivalent signals in spite of using spectrometer with different hardware features and is successfully used for the traceability and quantification of analytes in food samples. Nevertheless, to date only a few internationally agreed guidelines have been reported on the use of NMR for quantitative analysis. The main goal of the present study is to provide a methodological pipeline to assess the reproducibility of NMR data produced for a given matrix by spectrometers from different manufacturers, with different magnetic field strengths, age and hardware configurations. The results have been analyzed through a sequence of chemometric tests to generate a community-built calibration system which was used to verify the performance of the spectrometers and the reproducibility of the predicted sample concentrations.
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- 2020
12. Trans Lipid Library: Synthesis of Docosahexaenoic Acid (DHA) Monotrans Isomers and Regioisomer Identification in DHA-Containing Supplements
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Anna Sansone, Paolo Dambruoso, Chryssostomos Chatgilialoglu, Giorgia Giacometti, Carla Ferreri, Itziar Tueros, Georgia Menounou, Javier Amézaga, Roberta Scanferlato, Menounou, Georgia, Giacometti, Giorgia, Scanferlato, Roberta, Dambruoso, Paolo, Sansone, Anna, Tueros, Itziar, Amezaga, Javier, Chatgilialoglu, Chryssostomo, and Ferreri, Carla
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Quality Control ,0301 basic medicine ,Magnetic Resonance Spectroscopy ,Docosahexaenoic Acids ,Double bond ,Chemistry Techniques, Synthetic ,010402 general chemistry ,Toxicology ,01 natural sciences ,03 medical and health sciences ,Ingredient ,Omega-3 fatty acids, trans fatty acid isomers, isomer identification, deodorization, nutraceuticals ,Fish Oils ,Nutraceutical ,Isomerism ,trans fatty acid DHA ,Structural isomer ,Organic chemistry ,chemistry.chemical_classification ,Photolysis ,Chemistry ,food and beverages ,General Medicine ,0104 chemical sciences ,030104 developmental biology ,Docosahexaenoic acid ,Dietary Supplements ,Epoxy Compounds ,lipids (amino acids, peptides, and proteins) ,Isomerization ,Cis–trans isomerism ,Polyunsaturated fatty acid - Abstract
Docosahexaenoic acid (DHA) is a semiessential polyunsaturated fatty acid (PUFA) for eukaryotic cells that is found in natural sources such as fish and algal oils and widely used as an ingredient for omega-3 containing foods or supplements. DHA effects are connected to its natural structure with six cis double bonds, but geometrical monotrans isomers can be formed during distillation or deodorization processes, as an unwanted event that alters molecular characteristics and annihilates health benefits. The characterization of the six monotrans DHA regioisomers is an open issue to address for analytical, biological, and nutraceutical applications. Here we report the preparation, separation, and first identification of each isomer by a dual approach consisting of the following: (i) the direct thiyl radical-catalyzed isomerization of cis-DHA methyl ester and (ii) the two-step synthesis from cis-DHA methyl ester via monoepoxides as intermediates, which are separated and identified by nuclear magnetic resonance spectroscopy, followed by elimination for the unequivocal assignment of the double bond position. This monotrans DHA isomer library with NMR and GC analytical characterization was also used to examine the products of thiyl-radical-catalyzed isomerization of a fish oil sample and to evaluate the trans isomer content in omega-3 containing supplements commercially available in Italy and Spain.
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- 2018
13. Elucidating heteroatom influence on homonuclear
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Maria Giovanna, Chini, Daniele, Urbani, Paolo, Dambruoso, Raffaele, Riccio, and Giuseppe, Bifulco
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We report the structural dependency of long range scalar J-coupling constant across four bonds as function of the dihedral angles Φ1 and Φ3. The calculated homonuclear coupling constants
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- 2019
14. Professionals' behaviour and perceptions about searching and managing information at work: reviewed final version of survey
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Paolo Dambruoso, Ornella Russo, Silvana Mangiaracina, Irene Aguayo, Stefania Marzocchi, Danilo Brucchi, Chiara Pancaldi, Montse Montenero, Giovanni Crisonà, and Hessi Huttu
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Informazione scientifica ,Competenza Digitale - Abstract
L'indagine ha lo scopo di comprendere quale valore ha per i professionisti, ricercatori e R&D manager di aziende l'informazione tecnico-scientifica, frutto del lavoro di ricerca, e che uso fanno della documentazione scientifica (esigenze, comportamenti di ricerche, difficoltà di recupero o gestione, ecc.) durante il loro lavoro. La raccolta dati è stata fatta attraverso un questionario online e una serie di interviste semi strutturate e ha coinvolto tre paesi: Italia, Spagna e Finlandia.
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- 2019
15. Intercalation of Bioactive Molecules into Nanosized ZnAl Hydrotalcites for Combined Chemo and Photo Cancer Treatment
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Cecilia, Martini, Claudia, Ferroni, Gariboldi, MARZIA BRUNA, Anna, Donnadio, Annalisa, Aluigi, Giovanna, Sotgiu, Fabiola, Liscio, Paolo, Dambruoso, Maria Luisa Navacchia, and Tamara Postai and Greta Varchi
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- 2019
16. RM@Schools 3.0: A Wider Society Learning Project
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Raffaella Soave, Armida Torreggiani, Annalisa Aluigi, Alessandra Degli Esposti, Alberto Zanelli, Mirko Seri, Eleonora Polo, Paolo Dambruoso, Vladimiro Dal Santo, Anna Maria Ferretti, Laura Polito, Claudio Evangelisti, Alberto Bossi, and Maria Concetta Canino
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EIT Raw Materials ,RM@Schools 3.0 - Abstract
Raw Matters Ambassadors at schools 3.0 (RM@Schools) is an innovative program to make science education and careers in raw materials (RM) attractive for youngsters, involving RM experts and teachers (RM Ambassadors). ...
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- 2018
17. Validation of a 1D 1H-NOESY experiment for fingerprinting of grape juices
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Gallo, Vito, Nicola, Intini, Mastrorilli, Pietro, Latronico, Mario, Todisco, Stefano, Rizzuti, Antonino, Ragone, Rosa, Pasquale, Scapicchio, Paolo, Dambruoso, Mario Andrea Cremonini, Francesco, Benevelli, and Stefano, Ghelli
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- 2017
18. Supported Gold Nanoparticles for Alcohols Oxidation in Continuous Flow Heterogeneous Systems
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Maria Cristina Cassani, Davide Barreca, Adriana Mignani, Barbara Ballarin, Alessandro Massi, Elisa Boanini, Chiara Parise, Paolo Dambruoso, Anna Zaghi, Daniele Nanni, Ballarin, Barbara, Barreca, Davide, Boanini, Elisa, Cassani, Maria Cristina, Dambruoso, Paolo, Massi, Alessandro, Mignani, Adriana, Nanni, Daniele, Parise, Chiara, and Zaghi, Anna
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Materials science ,Gold nanoparticle ,Alkynyl carbamate-functionalized oxide supports ,General Chemical Engineering ,Inorganic chemistry ,Oxide ,Continuous-flow packed bed reactors ,010402 general chemistry ,Heterogeneous catalysis ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Heterogeneous catalysi ,Environmental Chemistry ,Gold nanoparticles ,Chemical Engineering (all) ,Alkynyl carbamate-functionalized oxide support ,Alcohol oxidation ,010405 organic chemistry ,Renewable Energy, Sustainability and the Environment ,Chemistry (all) ,Ambientale ,General Chemistry ,Flow chemistry ,0104 chemical sciences ,chemistry ,Chemical engineering ,Continuous-flow packed bed reactor ,Colloidal gold ,Triethoxysilane ,Surface modification - Abstract
Gold nanoparticles (AuNPs) were anchored on alkynyl carbamate-functionalized support materials having the suitable features for application as catalysts in continuous-flow packed bed reactors. The functionalization step was carried out by grafting with the difunctional organosilane [3-(2-propynylcarbamate)propyl]triethoxysilane (PPTEOS) three commercial micrometer-sized oxide supports, i.e. silica, alumina, and titania. The alkynyl-carbamate moieties were capable of straightforwardly reducing the gold precursor HAuCl4 yielding the supported AuNPs systems Au/SiO2@Yne, Au/Al2O3@Yne, and Au/ TiO2@Yne. A comparison among the three materials revealed that silica allowed the highest organic functionalization (12 wt %) as well as the highest gold loading (3.7 wt %). Moreover, a TEM investigation showed only for Au/SiO2@Yne the presence of homogeneously distributed spherically shaped AuNPs (average diameter 15 nm). Au/ SiO2@Yne is an efficient catalyst, both in batch and flow conditions, in the oxidation of a large variety of alcohols using H2O2 as the oxidizing agent at a temperature of 90 °C. Furthermore, under flow conditions, the catalyst worked for over 50 h without any significant decrease in the catalytic activity. The catalytic activity of the three catalysts was evaluated and compared in the oxidation of 1-phenylethanol as a model substrate. We found that the flow approach plays a strategic role in preserving the physical and chemical integrity of the solid catalysts during its use, with remarkable consequences for the reaction conversion (from 2% in batch to 80% in flow) in the case of Au/TiO2@Yne.
- Published
- 2017
19. Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma
- Author
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Spartaco Santi, Barbara Dozza, Enrico Lucarelli, A. Guerrini, Paolo Dambruoso, Davide Maria Donati, Giovanna Sotgiu, Claudia Ferroni, Marco Ballestri, Greta Varchi, Sandro Giannini, Serena Duchi, Duchi S, Sotgiu G, Lucarelli E, Ballestri M, Dozza B, Santi S, Guerrini A, Dambruoso P, Giannini S, Donati D, Ferroni C, and Varchi G
- Subjects
bone marrow ,Porphyrins ,Stromal cell ,Cell Survival ,Mesenchymal stem cells (MSC) ,Photodynamic therapy (PDT) ,medicine.medical_treatment ,Pharmaceutical Science ,Apoptosis ,Bone Neoplasms ,Photodynamic therapy ,Nanotechnology ,Osteosarcoma (OS) ,law.invention ,PHOTODYNAMIC THERAPY ,Drug Delivery Systems ,Confocal microscopy ,law ,Cell Line, Tumor ,medicine ,cancer ,Humans ,Photosensitizer ,Osteosarcoma ,Photosensitizing Agents ,Chemistry ,Nanoparticles (NPs) ,Mesenchymal stem cell ,Porphyrins (TPPS) ,Mesenchymal Stem Cells ,CHEMOTHERAPY ,Doxorubicin ,In vitro ,DRUG RESISTANCE ,Photochemotherapy ,Cell culture ,mesenchymal stromal cell ,Cancer research ,Nanoparticles ,Reactive Oxygen Species - Abstract
Mesenchymal stem cells (MSC) have the unique ability to home and engraft in tumor stroma. These features render them potentially a very useful tool as targeted delivery vehicles which can deliver therapeutic drugs to the tumor stroma. In the present study, we investigate whether fluorescent core-shell PMMA nanoparticles (FNPs) post-loaded with a photosensitizer, namely meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) and uploaded by MSC could trigger osteosarcoma (OS) cell death in vitro upon specific photoactivation. In co-culture studies we demonstrate using laser confocal microscopy and time lapse imaging, that only after laser irradiation MSC loaded with photosensitizer- coated fluorescent NPs (TPPS@FNPs) undergo cell death and release reactive oxygen species (ROS) which are sufficient to trigger cell death of all OS cells in the culture. These results encourage further studies aimed at proving the efficacy of this novel tri-component system for PDT applications. © 2013 Elsevier B.V.
- Published
- 2013
20. Enantioselective Organocatalytic Aryloxylation of beta-Keto Esters
- Author
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Filippo Sladojevich, Alessandro Ferrali, Kevin Greenaway, Paolo Dambruoso, Andrew J. Hazelwood, and Darren J. Dixon
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Cyclic compound ,biology ,010405 organic chemistry ,Stereochemistry ,Organic Chemistry ,Enantioselective synthesis ,Cinchona ,Cinchonine ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,Catalysis ,0104 chemical sciences ,3. Good health ,Quinone ,chemistry.chemical_compound ,chemistry ,Organocatalysis ,heterocyclic compounds ,Bifunctional ,Cinchonidine - Abstract
An efficient enantioselective aryloxylation of cyclic β-keto esters with a selection of substituted o-quinones catalyzed by a bifunctional cinchona alkaloid derivative, giving entry to tricyclic dioxin adducts with high enantio- and diastereoselectivity, is described. © Georg Thieme Verlag Stuttgart - New York.
- Published
- 2016
21. Elucidating new structural features of the triazole scaffold for the development of mPGES-1 inhibitors
- Author
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Carlo Pergola, Raffaele Riccio, Katrin Fischer, Oliver Werz, Vincenza Cantone, Paolo Dambruoso, Ines Bruno, Maria Giovanna Chini, Greta Varchi, Claudia Ferroni, Giuseppe Bifulco, and Antonella Pepe
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Pharmacology ,chemistry.chemical_classification ,inorganic chemicals ,musculoskeletal diseases ,Scaffold ,Chemistry ,Stereochemistry ,molecular modeling ,Organic Chemistry ,Triazole ,Pharmaceutical Science ,Biological activity ,mPGES-1 ,Biochemistry ,humanities ,chemistry.chemical_compound ,Enzyme ,Drug Discovery ,inhibitors ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,mpges-1 triazoli ,triazoles - Abstract
We report a new potent revisited version of a triazole-based inhibitor obtained by structure-based drug design on the human mPGES-1 crystal structure. Moreover, we disclosed the substitution with a halogen atom at position 5 as a new key factor influencing the biological activity on the mPGES-1 enzyme. This journal is
- Published
- 2015
22. Polymer nanoparticles with electrostatically loaded multicargo for combined cancer phototherapy
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Aurore Fraix, Andrea Guerrini, Paolo Dambruoso, Salvatore Sortino, Olimpia Coppellotti, Ilse Manet, Marco Ballestri, Greta Varchi, Giovanna Sotgiu, and Monica Camerin
- Subjects
chemistry.chemical_classification ,Materials science ,Singlet oxygen ,Biomedical Engineering ,Cationic polymerization ,Nanoparticle ,Nanotechnology ,General Chemistry ,General Medicine ,Polymer ,Photochemistry ,Porphyrin ,nanomedicine ,chemistry.chemical_compound ,chemistry ,photodynamic therapy ,Fluorescence microscope ,General Materials Science ,Derivative (chemistry) ,Visible spectrum - Abstract
We have developed a multifunctional polymer-based nanoplatform for bimodal cancer phototherapy. It was achieved by electrostatic entangling of two anionic photoactivable components, a commercial porphyrin and a tailored nitro-aniline derivative, within the cationic shell of polymeric nanoparticles (NPs) based on polymethyl methacrylate. The combination of steady-state and time-resolved spectroscopic and photochemical techniques shows that the two photoresponsive agents do not interfere with each other while being in close proximity in the same polymeric scaffold and can thus operate in parallel under the exclusive control of light stimuli. Specifically, visible light triggers satisfactory red fluorescence emission and generation of singlet oxygen (O-1(2)) from one component and release of nitric oxide (NO) from the other. Fluorescence microscopy analysis provides unambiguous evidence for the internalization of the NPs within B78H1 melanoma cells, where they induce amplified mortality due to a combinatory effect of the two photogenerated cytotoxic species.
- Published
- 2015
23. TPPS supported on core–shell PMMA nanoparticles: the development of continuous- flow membrane-mediated electrocoagulation as a photocatalyst processing method in aqueous media
- Author
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Giovanna Sotgiu, Andrea Guerrini, Marco Ballestri, Greta Varchi, Paolo Dambruoso, Claudia Ferroni, and Alessandro Massi
- Subjects
magnetic nanoparticles ,room-temperature ,visible-light ,recyclable catalysts ,flow chemistry ,Inorganic chemistry ,Electrochemistry ,Catalysis ,law.invention ,batch ,versatile ,chemistry.chemical_compound ,Colloid ,law ,Tetraphenylporphyrin ,Environmental Chemistry ,Photocatalysis ,Electrolysis ,Electrolysis of water ,PMMA-NPs ,Chemistry ,technology, industry, and agriculture ,Ambientale ,Diels-alder reactions ,Pollution ,highly efficient ,organocatalysts ,immobilization ,Membrane - Abstract
Herein, the successful utilization of core shell poly-methyl methacrylate nanoparticles (CS-PMMA NPs) as a valuable support for organocatalyst immobilization in aqueous medium is presented along with an unprecedented direct current-based electrochemical method for processing a water colloid of the resulting nano-supported catalyst. The NP coagulation procedure relied on various electrocoagulation techniques and it was achieved through water electrolysis occurring in a dialysis membrane immersed in the colloid. Mass recoveries of up to 95% w/w were obtained for the NPs within 1 h of electrolysis. This was optimized at a constant current of 90 mA or a constant potential of 24 V. The disclosed NP separation procedure was effectively exploited in a continuous-flow reaction/separation/recycle sequence involving the tetraphenylporphyrin tetrasulfonate (TPPS) organo-photocatalyst immobilized on CS-PMMA NPs (NP2s). After an optimization study aimed at maximizing the chemical efficiency of the entire synthetic and purification process, the nano-supported catalyst NP2 (0.05 mol%) efficiently promoted the photo-excitation of triplet oxygen to singlet oxygen for the selective and environmentally benign sulfoxidation of a model sulfide in an aqueous medium.
- Published
- 2015
24. 2′-Methyl taxanes: synthesis and NMR configurational assignment
- Author
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Paolo Dambruoso, Giovanni Appendino, Andrea Guerrini, Luigi Gomez-Paloma, Gabriele Fontana, Giuseppe Bifulco, Arturo Battaglia, and Carla Bassarello
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NMR spectra database ,Stereochemistry ,Chemistry ,Organic Chemistry ,Drug Discovery ,Context (language use) ,Biochemistry ,Stereocenter - Abstract
Capitalizing on an oxidation–alkylation approach, a non-diastereoselective entry into 2′-methyl taxanes was developed. The issue of configurational assignment at the newly formed side-chain quaternary stereocenter was solved and put into a more general context by integrating information from an alternative diastereoselective synthesis of model compounds and from spectroscopic measurements, critically comparing the J -Based and the Universal NMR Database approaches.
- Published
- 2005
25. The Reductive Fragmentation of 7-Hydroxy-9,10-dioxotaxoids
- Author
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Giovanni Appendino, Alain Noncovich, Piergiorgio Bettoni, Ezio Bombardelli, Gabriele Fontana, Paolo Dambruoso, and Olov Sterner
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Cerium ,Fragmentation (mass spectrometry) ,chemistry ,Organic Chemistry ,chemistry.chemical_element ,Organic chemistry ,Biological activity ,Physical and Theoretical Chemistry - Abstract
The retro-aldol reductive fragmentation of different structural types of 7-hydroxy-9,10-dioxotaxoids was investigated, showing that the reaction is typical of taxanes and requires cerium(III) promotion with NaBH4 in protic medium and alkylboron (aluminium) hydrides in aprotic solvents. The resulting 7,8-seco-taxanes are key intermediates for the synthesis of a novel class of anticancer taxanes endowed with a unique pattern of in vivo biological activity. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)
- Published
- 2003
26. Percutaneous Coronary Sinus Catheterization With the ProPlege Catheter Under Transesophageal Echocardiography and Pressure Guidance
- Author
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Giuseppe Labriola, Cataldo Labriola, Domenico Paparella, Maurizio Braccio, Pier Paolo Dambruoso, and Francesco Greco
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Male ,medicine.medical_specialty ,Cardiac Catheterization ,Percutaneous ,Perforation (oil well) ,Cardiac Catheters ,Minimally invasive cardiac surgery ,Pressure ,Medicine ,Fluoroscopy ,Humans ,Minimally Invasive Surgical Procedures ,Coronary sinus ,Aged ,Retrospective Studies ,medicine.diagnostic_test ,business.industry ,Coronary Sinus ,Equipment Design ,Middle Aged ,Surgery ,Catheter ,Anesthesiology and Pain Medicine ,Right heart ,Female ,Radiology ,Cardiology and Cardiovascular Medicine ,business ,Complication ,Echocardiography, Transesophageal - Abstract
Objectives Percutaneous catheterization of the coronary sinus (CS) to enable the administration of retrograde cardioplegia may play an important role in minimally invasive cardiac surgery. A new specially designed device (ProPlege; Edwards Lifesciences, Irvine, CA) is described that can be placed under transesophageal echocardiography (TEE) and pressure guidance with a high rate of success and low rate of complications. Design Case series. Setting A university-affiliated private hospital. Participants Patients undergoing minimally invasive cardiac surgery. Interventions The ProPlege device was placed under TEE and pressure guidance only. Measurements and Main Results Records of 70 patients managed with ProPlege were reviewed and analyzed. Successful placement was attained in 69 patients (98.6%) as confirmed by the ventricularization of the CS pressure curve and TEE images. Direct imaging of the ProPlege tip was possible in 34 patients (49.2%). The capacity to generate a CS pressure>30 mmHg during retrograde cardioplegia administration at a flow>150 mL/min was obtained in 64 patients; ProPlege displacement occurred in 5 cases (7.2%). Successful retrograde cardioplegia was delivered in 91.4% of cases. No CS perforation or other injuries to the right heart were noted at intraoperative TEE or direct surgical inspection. Conclusions Percutaneous CS catheterization with ProPlege was performed with a high rate of success for positioning and low complication rate using TEE and pressure guidance only. Further studies are needed to more accurately determine complication rates and to establish the possible complementary role of fluoroscopy.
- Published
- 2014
27. Thiophene-Based Compounds as Fluorescent Tags to Study Mesenchymal Stem Cell Uptake and Release of Taxanes
- Author
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Giovanna Sotgiu, Greta Varchi, Paolo Dambruoso, Enrico Lucarelli, Valentina Coccè, Elisa Martella, Serena Duchi, Augusto Pessina, Arianna Bonomi, A. Guerrini, S. Duchi, P. Dambruoso, E. Martella, G. Sotgiu, A. Guerrini, E. Lucarelli, A. Pessina, V. Coccè, A. Bonomi, and G. Varchi
- Subjects
Stromal cell ,Stereochemistry ,Biomedical Engineering ,Molecular Conformation ,Pharmaceutical Science ,Bioengineering ,Thiophenes ,PACLITAXEL ,Exosomes ,Exosome ,chemistry.chemical_compound ,conjugation chemistry ,Drug Delivery Systems ,Cell Line, Tumor ,Fluorescence microscope ,Humans ,Fluorescent Dyes ,Pharmacology ,taxol ,mesenchymal stem cells ,Chemistry ,Organic Chemistry ,Mesenchymal stem cell ,Biological Transport ,mesechymal stem cell ,CANCER ,In vitro ,Microvesicles ,Cell biology ,Spectrometry, Fluorescence ,Paclitaxel ,Cell culture ,Biotechnology - Abstract
Human mesenchymal stem cells (hMSC) are multipotent cells that display the unique ability to home and engraft in tumor stroma. This remarkable tumor tropic property has generated a great deal of interest in many clinical settings. Recently, we showed that hMSC represent an excellent base for cell-mediated anticancer therapy since they are able to internalize paclitaxel (PTX) and to release it in an amount sufficient to inhibit tumor cell proliferation. In order to shed light on the dynamics of drug uptake and release, in the present paper we describe the synthesis of two novel thiophene-based fluorophore-paclitaxel conjugates, namely PTX-F32 and PTX-F35, as tools for in vitro drug tracking. We aimed to study the ability of these novel derivatives to be efficiently internalized by hMSC and, in a properly engineered coculture assay, to be released in the medium and taken up by tumor cells. In order to ensure better stability of the conjugates toward enzymatic hydrolysis, the selected oligothiophenes were connected to the taxol core at the C7 position through a carbamate linkage between PTX and the diamino linker. Antiproliferative experiments on both tumor cells and stromal cells clearly indicate that, in good correlation with the parent compound, cells are sensitive to nanomolar concentrations of the fluorescent conjugates. Moreover, in the coculture assay we were able to monitor, by fluorescence microscopy, PTX-F32 trafficking from hMSC toward glioblastoma U87 tumor cells. Our work paves the way for novel possibilities to perform extensive and high quality fluorescence-based analysis in order to better understand the cellular mechanisms involved in drug trafficking, such as microvescicle/exosome mediated release, in hMSC vehicle cells. © 2014 American Chemical Society.
- Published
- 2014
28. ChemInform Abstract: Enantioselective Organocatalytic Aryloxylation of β-Keto Esters
- Author
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Alessandro Ferrali, Kevin Greenaway, Filippo Sladojevich, Darren J. Dixon, Paolo Dambruoso, and Andrew J. Hazelwood
- Subjects
Chemistry ,Enantioselective synthesis ,Organic chemistry ,General Medicine - Published
- 2011
29. Determination of relative configuration in organic compounds by NMR spectroscopy and computational methods
- Author
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Paolo Dambruoso, Giuseppe Bifulco, Raffaele Riccio, and Luigi Gomez-Paloma
- Subjects
Magnetic Resonance Spectroscopy ,Chemistry ,Analytical chemistry ,Molecular Conformation ,General Medicine ,General Chemistry ,Fluorine-19 NMR ,Nuclear magnetic resonance spectroscopy ,Molecular conformation ,Databases as Topic ,Models, Chemical ,Computational chemistry ,Solvents ,Physical chemistry ,Quantum Theory ,Computer Simulation ,Organic Chemicals - Published
- 2007
30. Total Synthesis of Natural Products
- Author
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Ines Bruno, Paolo Dambruoso, Stefania Terracciano, and Giuseppe Bifulco
- Subjects
General Medicine - Published
- 2007
31. Advances in the universal NMR database approach. 2'-substituted taxanes as probes for an improved protocol of diastereomeric differentiation
- Author
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Giuseppe Bifulco, Luigi Gomez-Paloma, Paolo Dambruoso, Arturo Battaglia, Carla Bassarello, Giovanni Appendino, and Gabriele Fontana
- Subjects
Basis (linear algebra) ,Series (mathematics) ,Databases, Factual ,Molecular Structure ,Chemistry ,Organic Chemistry ,Diastereomer ,Stereoisomerism ,Biochemistry ,Combinatorial chemistry ,Set (abstract data type) ,NMR spectra database ,Moiety ,Taxoids ,Physical and Theoretical Chemistry ,Protocol (object-oriented programming) ,Nuclear Magnetic Resonance, Biomolecular - Abstract
[structure: see text] The configuration of the alpha-substituted alpha-hydroxy-beta-aminoester moiety in a series of 2'-substituted taxanes was analyzed according to the recently proposed Universal NMR Database (UDB) approach. A critical analysis of the results showed that modifications regarding chemical shift adjustment (so as to render the shifts virtually connectivity independent) were necessary to get consistent stereoassignments in this set of compounds. On this basis, a modified UDB-based strategy, especially tailored to the configurational assignment of densely substituted diastereomeric fragments, is proposed.
- Published
- 2005
32. Efficiency in isotetronic acid synthesis via a diamine-acid couple catalyzed ethyl pyruvate homoaldol reaction
- Author
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Paolo Dambruoso, Alessandro Dondoni, and and Alessandro Massi
- Subjects
Organic Chemistry ,Azomethine ylide ,Biochemistry ,Cycloaddition ,Pyrrolidine ,Catalysis ,chemistry.chemical_compound ,chemistry ,Aldol reaction ,Diamine ,Reagent ,Trifluoroacetic acid ,Organic chemistry ,Physical and Theoretical Chemistry - Abstract
[reaction: see text] L-proline failed to act as an organocatalyst in the homoaldol reaction of ethyl pyruvate; however, it reacted with the ester to give an azomethine ylide that in turn underwent 1,3-dipolar cycloaddition with a second molecule of pyruvate. Direct catalytic homoaldol reaction of ethyl pyruvate was performed using an (S)-(+)-1-(2-pyrrodinylmethyl)pyrrolidine/trifluoroacetic acid combination as organocatalyst. The use of polymer-supported reagents allowed for the lactonization of the aldol and isolation of the isotetronic acid derivative in hydroxy-free form.
- Published
- 2005
33. Biostructural analysis of alkylated c-2 ' Taxol analogues
- Author
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Johnson, Sa, Thomas, S., paolo dambruoso, Appendino, G., Giannakakou, P., and Snyder, Jp
34. Thiazolium-Functionalized Polystyrene Monolithic Microreactors for Continuous-Flow Umpolung Catalysis
- Author
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Pier Paolo Giovannini, Salvatore Pacifico, Alberto Cavazzini, Lorenzo Caciolli, Alessandro Massi, Paolo Dambruoso, Olga Bortolini, and Daniele Ragno
- Subjects
organic sybthesis ,polymer ,Stetter reaction ,bound thiamine models ,Pollution ,Combinatorial chemistry ,Umpolung ,Catalysis ,NO ,Benzaldehyde ,bound thiamine models, solid supported organocatalyst, benzoin condensation, carbene catalysis, organic sybthesis, polymer ,chemistry.chemical_compound ,chemistry ,carbene catalysis ,Environmental Chemistry ,Organic chemistry ,solid supported organocatalyst ,Polystyrene ,Benzoin condensation ,Microreactor ,Carbene ,benzoin condensation - Abstract
Thiazolium salt pre-catalysts have been immobilized on silica and monolithic polystyrene and their activity was tested under batch conditions in three model umpolung reactions, namely the benzoin condensation of benzaldehyde, the acyloin-type condensation of biacetyl, and the Stetter reaction of biacetyl with trans-chalcone. A prerequisite of the study has been the utilization of environmentally benign water and ethanol solvents. After having established the higher performance of polystyrene monolithic thiazolium carbene catalysts, their effectiveness has been tested under the flow regime by fabricating the corresponding monolithic microreactors (pressure-resistant stainless-steel columns). Importantly, it has been demonstrated by a brief substrate scope study that the polymeric matrix and the continuous flow regime synergistically contribute to preserve the activity of the carbene catalysts over time, thus permitting the long-term operation (up to 7 days) of the prepared monolithic reactors for the production of valuable compounds via the umpolung strategy.
Catalog
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