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One pot synthesis of new cross-linked chitosan-Schiff' base: Characterization, and anti-proliferative activities
- Source :
- International Journal of Biological Macromolecules. 184:558-565
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Four novel chitosan hydrogels were successfully synthesized through the cross-linking reaction of chitosan with different concentrations of ethyl 5-(3,5-dihydroxy-1,4-dioxan-2-yl)-2-methylfuran-3-carboxylate. Their structures were confirmed by Fourier transform infrared spectroscopy (FT-IR), 13C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy (CP/MAS 13C NMR), ultraviolet-visible spectroscopy, thermogravimetric analysis (TGA, DTA), and X-ray diffraction (XRD). Cytotoxicity on hepatocellular carcinoma (HepG-2) cell line and a normal African green monkey kidney (Vero) cell line were studied using the MTT assay. The resultant hydrogels showed a good inhibitory effect comparing to the un-modified parent; the hydrogels with the lowest degree cross-linking (0.125 and 0.25 mol cross-linker per one chitosan residue) showed potent anticancer activity in the HepG2 cells with IC50 of 57.9 and 80.9 μg/ml, respectively. These results show that the newly synthesized cross-linked chitosan derivatives demonstrated more selectivity to the HepG2 than the Vero cells, indicating its potential for Investigation in the cure of hepatocellular carcinoma.
- Subjects :
- Cell Survival
Carboxylic Acids
Antineoplastic Agents
02 engineering and technology
Biochemistry
Chitosan
03 medical and health sciences
chemistry.chemical_compound
X-Ray Diffraction
Structural Biology
Chlorocebus aethiops
Spectroscopy, Fourier Transform Infrared
Magic angle spinning
Animals
Humans
MTT assay
Fourier transform infrared spectroscopy
Vero Cells
Molecular Biology
Schiff Bases
Cell Proliferation
030304 developmental biology
0303 health sciences
technology, industry, and agriculture
Hydrogels
Hep G2 Cells
General Medicine
Nuclear magnetic resonance spectroscopy
Carbon-13 NMR
021001 nanoscience & nanotechnology
chemistry
Self-healing hydrogels
Vero cell
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 184
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....e782cde3da81521da1ccda41a0c1458c