1. In vivo study of dermal collagen of striae distensae by confocal Raman spectroscopy
- Author
-
Airton Abrahão Martin, Carla Abdo Brohem, Pam Wen Lung, Desiree Cigaran Schuck, Márcio Lorencini, Claudio A. Téllez-Soto, Thiago de Oliveira Mendes, and Vamshi Krishna Tippavajhala
- Subjects
Adult ,Confocal ,Human skin ,Dermatology ,Spectrum Analysis, Raman ,01 natural sciences ,030207 dermatology & venereal diseases ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Dermis ,In vivo ,medicine ,Humans ,Striae distensae ,Bound water ,Least-Squares Analysis ,integumentary system ,Chemistry ,010401 analytical chemistry ,Discriminant Analysis ,Water ,medicine.disease ,0104 chemical sciences ,medicine.anatomical_structure ,symbols ,Biophysics ,Female ,Surgery ,Collagen ,Striae Distensae ,Raman spectroscopy ,Confocal raman spectroscopy - Abstract
This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.
- Published
- 2018