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A sample representing a population of the Florence district of middle 19th century was studied to determine the age of occurrence of enamel hypoplasias. The age interval most affected was that between 1.5 and 3.5 years. Historical sources on weaning habits of 19th-century Italian populations indicate a weaning period between 12 and 18 months. This is in agreement with the data on enamel defects, showing that children of post-weaning age are more subject to stress. Wide 'grooves', with prolonged duration, are concentrated between 2 and 2.5 years, whereas 'lines' occur primarily between 2.5 and 3 years. We suggest that this distribution could reflect the gradual introduction of dietary supplements until weaning is complete.

The Monti 'e Prama site, made famous by the discovery of numerous large stone statues "The Monti 'e Prama giants", is a necropolis located in Sardinia, dated to the X - IX BC. The statues, all depicting men: archers, boxers and fencers, were found collapsed and fragmented, in the immediate vicinity of the burials. The sample of inhumated skeletons consists of 41 individuals, almost all males, who died when adolescents, young adults or, more rarely, mature. Neither children nor elders were present. An interesting question is whether the statues depicted the deceased or could have come from a temple located elsewhere. In this regard, the diagnosis of the age at death is an element of considerable importance, but the skeletal age is difficult to determine in this sample because of the bad state of preservation of the bones, while the teeth are well preserved and have allowed us to reconstruct a seriation in order to seniority on the basis of wear. Since tooth wear is a weak indicator of age, we also used a method based on the deposition of secondary dentine, by the pulp area/tooth area ratio calculation, which gets a greater precision. It was found that the correlation between the degree of wear and pulp area/tooth area ratio is somewhat low, indicating that the two processes, although both age-dependent, do not have a very similar trend. It was also noted that the method of pulp area / tooth area ratio tends to "make the individuals grow old" in cases of discrepancy, but in general confirms the young age of many adults and the absence of the elderly. In this sense, it confirms even the possibility of an association between the deceased and the statues of young athletes or warriors. The results of pulp area/tooth area ratio method, by moving the sample as a whole to ages slightly more advanced than those suggested by wear, offers the hypothesis that the community was characterized by alimentary habits involving a little wearing mastication activity. [ABSTRACT FROM AUTHOR]

We recently presented the case of a first century AD young woman, found in the archaeological site of Cosa, showing clinical signs of malnutrition, such as short height, osteoporosis, dental enamel hypoplasia and cribra orbitalia, indirect sign of anemia, all strongly suggestive for celiac disease (CD). However, whether these findings were actually associated to CD was not shown based on genetic parameters. To investigate her human leukocyte antigen (HLA) class II polymorphism, we extracted DNA from a bone sample and a tooth and genotyped HLA using three HLA-tagging single nucleotide polymorphisms for DQ8, DQ2.2 and DQ2.5, specifically associated to CD. She displayed HLA DQ 2.5, the haplotype associated to the highest risk of CD. This is the first report showing the presence of a HLA haplotype compatible for CD in archaeological specimens.